CN105062677A - Method for preparing fermented beet extract through processing beet juice by Lactobacillus plantarum - Google Patents
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Abstract
The invention discloses a method for preparing fermented beet extract through processing a beet juice by Lactobacillus plantarum. The method comprises the following steps: preparing the beet juice, and preparing a Lactobacillus plantarum solution; inoculating the Lactobacillus plantarum solution to the beet juice, and fermenting; and filtering the obtained beet fermentation solution, separating, and carrying out reduced pressure concentration to obtain the fermented beet extract. The fermented beet extract prepared in the invention has the delicate fragrance of beet, and pleasant sweet and mellow fragrance; and the fermented beet extract is added to cigarette in order to increase the delicate fragrance of the cigarette, soften fine smoke, reduce irritation of the cigarette and reduce residues, so the quality of the cigarette is improved, and application of the beet extract in the cigarette is realized. The method is a new approach for economically, efficiently and directionally producing natural cigarette perfumes, develops novel characteristic cigarette perfumes with excellent properties, which cannot be produced through traditional perfume production methods, and increases the kind of the cigarette perfumes.
Description
Technical field
The present invention relates to the application of microbial fermentation technology, also relate to the technical fields such as the preparation of essence spice for cigarette, specifically a kind of plant lactobacillus process sugar beet juice prepares the method for fermented type beet medicinal extract.
Background technology
Beet, biennial herb plant, root is coniform to fusiform, succulence.The block root moisture of beet accounts for 75%, and solid substance accounts for 25%.In solid substance, sucrose accounts for 16% ~ 18%, and nonsugar accounts for 7% ~ 9%.Nonsugar is divided into again solubility and insoluble two kinds: insoluble non-saccharide is Mierocrystalline cellulose, hemicellulose, protopectin and protein mainly; Solubility non-saccharide is divided into again inorganic non-saccharide and organic non-saccharide.Beet tails is the main source of edible granulated sugar.Sugar is the indispensable nutritive substances of people's lives, is also the important source material of foodstuffs industry, drink industry and medicine industry.Beet has antitumor, that guarantor's stomach controls liver, fat-reducing liver-protecting, step-down health-care effect.
The glucide that the block root of beet is rich in because of it, is with a wide range of applications in essence and flavoring agent industry.Be that cigarette perfume material prepared by raw material with beet, there is soft fine and smooth flue gas in cigarette, reduce the effect stimulated.But, required cigarette garden beet medicinal extract can not be produced by traditional production of flavor method.And, be that the method for prepared using microbial fermentation technology production characteristic tobacco aromatics using has no report with beet.
Microbial fermentation technology for core, utilizes the Metabolic activity process of microorganism with modern fermentation technique, manufactures various industrial fermentation product through bio-transformation on a large scale.Fermentation is by carrying out large-scale grown cultures to microorganism (or animal and plant cells), makes it chemical transformation and physiological change occur, thus produces and accumulate a large amount of people and to ferment the process of required meta-bolites.Fermentation is the distinctive effect of microorganism.Microbial project, namely refers to fermentation engineering, refers to adopt modern project technique means, and utilizing some specific function of microorganism, is the product that human being's production is useful, or directly a kind of technology of microbe application in Industrial processes.Microbial project is the general name of large scale fermentation production technique, and the achievement that it absorbs genetically engineered, cell engineering and enzyme engineering and other technologies on zymotechnique basis is formed.Fermentation engineering is close with many Domain relations such as chemical industry, medicine, food, the energy, environment protection and husbandry, has very large economic benefit.
Plant lactobacillus is the one of milk-acid bacteria, and optimum growth temperature is 30 ~ 35, anaerobism or amphimicrobian, and optimal pH about 6.5, belongs to homofermentative lactic bacteria, belongs to chemoheterotrophic bacteria, and energy ferment pentoses or gluconate, in end product, more than 85% is lactic acid.Plant lactobacillus has a lot of health-care effects: 1, have certain immunoregulation effect; 2, restraining effect is had to pathogenic bacterium; 3, serum cholesterol content and preventing cardiovascular disease is reduced; 4, intestinal flora balance is maintained; 5, absorption of nutrient ingredients is promoted; 6, lactose intolerance is alleviated; 7, the formation etc. of inhibition tumor cell.
Summary of the invention
The object of the invention is to the blank for prior art, provide a kind of method that plant lactobacillus process sugar beet juice prepares fermented type beet medicinal extract.The present invention adopts the biotechnological formulation made by plant lactobacillus (Lactobacillusplantarum) PJH05 screened in the offal piled up from principal and subordinate, is inoculated in sugar beet juice, have developed cigarette fermented type beet medicinal extract.This beet medicinal extract has the delicate fragrance note of beet, and with pleasant sweet and pure fragrance.
The object of the invention is to realize by following technical solution: a kind of plant lactobacillus process sugar beet juice prepares the method for fermented type beet medicinal extract, it is characterized in that, comprises the following steps:
(1) preparation of sugar beet juice:
Fresh beet is added in clear water and cleans up, then carry out making beating process, collect slurries, obtain beet slurries; In gained beet slurries, add the Nacl that the glucose that weight is beet slurry weight 0.1% ~ 5% and weight are beet slurry weight 0.01% ~ 0.5%, mix rear sterilizing, obtain sugar beet juice;
(2) preparation of plant lactobacillus bacterium liquid:
Plant lactobacillus (Lactobacillusplantarum) PJH05 is added to the water preparation bacterium liquid, obtains plant lactobacillus bacterium liquid; Described plant lactobacillus (Lactobacillusplantarum) PJH05 is deposited in the China typical culture collection center (CCTCC) being positioned at Hubei China province wuchang, wuhan Luo Jia Shan Wuhan University on January 5th, 2012, preserving number is: CCTCCNO:M2012002;
(3) ferment:
Be 0.1 ~ 1% by the mass ratio of plant lactobacillus bacterium liquid and sugar beet juice, gained plant lactobacillus bacterium liquid be inoculated in the sugar beet juice after sterilizing, obtain postvaccinal sugar beet juice; Postvaccinal for gained sugar beet juice is fermented, obtains beet fermented liquid;
(4) filtering separation:
Gained beet fermented liquid is carried out filtering separation, obtains the beet filtrate of clarifying;
(5) concentrating under reduced pressure:
Gained beet filtrate is carried out concentrating under reduced pressure, and obtaining relative density is 1.150 ~ 1.350g/cm
3fermented type beet medicinal extract.
As preferably, the sterilizing in described step (1) is that sugar beet juice is carried out sterilising treatment 15 ~ 30min at 100 ~ 115 DEG C.
As preferably, the water in described step (2) is the sterilized water of 100 times of quality.
As preferably, the fermentation in described step (3) is first 2.5 ~ 5.5 in pH value by postvaccinal for gained sugar beet juice, and mixing speed is 200 ~ 500rpm, and air flow is 1 ~ 3VVM, and leavening temperature is the condition bottom fermentation 1d ~ 7d of 15 DEG C ~ 30 DEG C; Then be the condition bottom fermentation 7d ~ 28d of 5 DEG C ~ 15 DEG C at leavening temperature.
As preferably, the filtering separation in described step (4) is that gained beet fermented liquid is first used filtered through gauze, more again filters with the filter membrane of 0.8 micron.
As preferably, the concentrating under reduced pressure temperature in described step (5) is 50 ~ 80 DEG C, and concentrated pressure is 0.01 ~ 0.2 normal atmosphere.
Advantage of the present invention is:
1, the fermented type beet medicinal extract prepared by the present invention has the delicate fragrance note of beet, and with pleasant sweet and pure fragrance; Added in cigarette, the delicate fragrance note of cigarette can be increased, soft fine and smooth flue gas, reduced the pungency of cigarette, reduce residual, thus improve the quality of beet medicinal extract, achieve the application of beet medicinal extract in cigarette.
2, the present invention opens an economy, the efficient and new way of the natural tobacco aromatics using of orientable production, have developed the not fertile colory novel characteristic tobacco aromatics using of traditional production of flavor method, adds the category of tobacco aromatics using.
Embodiment
Below in conjunction with specific embodiment, Relational Questions of the present invention is described in further detail.
Embodiment 1:
1, the preparation of sugar beet juice:
(1) the fresh beet getting 5kg adds in clear water and cleans up, and then utilizes juice extractor beet to be carried out making beating process, collects slurries, obtain beet slurries.
(2) in gained beet slurries, add the Nacl that the glucose that weight is beet slurry weight 0.1% and weight are beet slurry weight 0.01%, mix, obtain sugar beet juice.
(3) by prepared sugar beet juice sterilizing 30min at 100 DEG C, the sugar beet juice after sterilizing is obtained.
2, the preparation of plant lactobacillus bacterium liquid:
Plant lactobacillus (Lactobacillusplantarum) PJH05 getting 10g adds in the sterilized water of 100 times of quality and prepares bacterium liquid for fermentative processing, obtains plant lactobacillus bacterium liquid.
3, ferment:
(1) inoculate: aseptically, be 0.1% by the mass ratio of plant lactobacillus bacterium liquid and sugar beet juice, plant lactobacillus bacterium liquid be inoculated in sugar beet juice, obtain postvaccinal sugar beet juice.
(2) be 2.5 by postvaccinal for gained sugar beet juice in pH value, mixing speed is 200rpm, and air flow is 1VVM, and leavening temperature is the condition bottom fermentation 7d of 15 DEG C; Then be the condition bottom fermentation 28d of 5 DEG C at leavening temperature, obtain beet fermented liquid.
4, filtering separation:
(1) coarse filtration: by the oarse-grained impurity of gained beet fermented liquid gauze elimination, obtain beet coarse filtration liquid.
(2) membrane filtration: again filtered by the filter membrane of gained beet coarse filtration liquid with 0.8 micron, obtains the beet filtrate of clarifying.
5, concentrating under reduced pressure:
Gained beet filtrate is carried out concentrating under reduced pressure, and wherein thickening temperature is 50 DEG C, and concentrated pressure is 0.012 normal atmosphere, and obtaining relative density after concentrated is 1.1839g/cm
3fermented type beet medicinal extract 1.
Embodiment 2:
1, the preparation of sugar beet juice:
(1) the fresh beet getting 5kg adds in clear water and cleans up, and then utilizes juice extractor beet to be carried out making beating process, collects slurries, obtain beet slurries.
(2) in gained beet slurries, add the Nacl that the glucose that weight is beet slurry weight 1% and weight are beet slurry weight 0.1%, mix, obtain sugar beet juice.
(3) by prepared sugar beet juice sterilizing 20min at 110 DEG C, the sugar beet juice after sterilizing is obtained.
2, the preparation of plant lactobacillus bacterium liquid:
Plant lactobacillus (Lactobacillusplantarum) PJH05 getting 10g adds in the sterilized water of 100 times of quality and prepares bacterium liquid for fermentative processing, obtains plant lactobacillus bacterium liquid.
3, ferment:
(1) inoculate: aseptically, be 0.5% by the mass ratio of plant lactobacillus bacterium liquid and sugar beet juice, plant lactobacillus bacterium liquid be inoculated in sugar beet juice, obtain postvaccinal sugar beet juice.
(2) be 4 by postvaccinal for gained sugar beet juice in pH value, mixing speed is 300rpm, and air flow is 2VVM, and leavening temperature is the condition bottom fermentation 5d of 20 DEG C; Then be the condition bottom fermentation 15d of 10 DEG C at leavening temperature, obtain beet fermented liquid.
4, filtering separation:
(1) coarse filtration: by the oarse-grained impurity of gained beet fermented liquid gauze elimination, obtain beet coarse filtration liquid.
(2) membrane filtration: again filtered by the filter membrane of gained beet coarse filtration liquid with 0.8 micron, obtains the beet filtrate of clarifying.
5, concentrating under reduced pressure:
Gained beet filtrate is carried out concentrating under reduced pressure, and wherein thickening temperature is 60 DEG C, and concentrated pressure is 0.1 normal atmosphere, and obtaining relative density after concentrated is 1.250g/cm
3fermented type beet medicinal extract 2.
Embodiment 3:
1, the preparation of sugar beet juice:
(1) the fresh beet getting 5kg adds in clear water and cleans up, and then utilizes juice extractor beet to be carried out making beating process, collects slurries, obtain beet slurries.
(2) in gained beet slurries, add the Nacl that the glucose that weight is beet slurry weight 5% and weight are beet slurry weight 0.5%, mix, obtain sugar beet juice.
(3) by prepared sugar beet juice sterilizing 15min at 115 DEG C, the sugar beet juice after sterilizing is obtained.
2, the preparation of plant lactobacillus bacterium liquid:
Plant lactobacillus (Lactobacillusplantarum) PJH05 getting 10g adds in the sterilized water of 100 times of quality and prepares bacterium liquid for fermentative processing, obtains plant lactobacillus bacterium liquid.
3, ferment:
(1) inoculate: aseptically, be 1% by the mass ratio of plant lactobacillus bacterium liquid and sugar beet juice, plant lactobacillus bacterium liquid be inoculated in sugar beet juice, obtain postvaccinal sugar beet juice.
(2) be 5.5 by postvaccinal for gained sugar beet juice in pH value, mixing speed is 500rpm, and air flow is 3VVM, and leavening temperature is the condition bottom fermentation 1d of 30 DEG C; Then be the condition bottom fermentation 7d of 15 DEG C at leavening temperature, obtain beet fermented liquid.
4, filtering separation:
(1) coarse filtration: by the oarse-grained impurity of gained beet fermented liquid gauze elimination.Obtain beet coarse filtration liquid.
(2) membrane filtration: again filtered by the filter membrane of gained beet coarse filtration liquid with 0.8 micron, obtains the beet filtrate of clarifying.
5, concentrating under reduced pressure:
Gained beet filtrate is carried out concentrating under reduced pressure, and wherein thickening temperature is 80 DEG C, and concentrated pressure is 0.2 normal atmosphere, and obtaining relative density after concentrated is 1.350g/cm
3fermented type beet medicinal extract 3.
The applied research of beet medicinal extract in cigarette
For the beet medicinal extract 1 prepared by embodiment 1, carry out the explanation of applied research in cigarette.
1, the physical index of beet medicinal extract detects:
As follows by the physical index of the beet medicinal extract of above-mentioned process exploitation:
| Title | Cigarette garden beet medicinal extract |
| Outward appearance | Brown half mobility paste |
| Fragrance | Fragrant and sweet |
| Relative density (20/20 DEG C) | 1.1839 |
| Refractive index (20 DEG C) | 1.4146 |
| Miscible degree (25 DEG C) in ethanol | This extract of 1 volume dissolves in 1-20 v aqueous solution |
| Lead content (mg/kg) | ≤5.0 |
| Arsenic content (mg/kg) | ≤1.0 |
2, the cigarette garden beet medicinal extract of preparation is evaluated by the following method its effect in cigarette: get certain density beet medicinal extract, join (solvent is water) in the high, medium and low pipe tobacco of 200 grams.After sealed plastic bag sealing, putting into baking oven and dry at 50 DEG C after 30 minutes, is 22 ± 1 DEG C in temperature, and humidity is balance 2 hours under 60 ± 1% environment, then rolls into cigarette, does contrast smoke panel test with the blank cigarette not adding beet medicinal extract.
7 people's compositions are smoked panel test group, and wherein national level is smoked panel test Shi Yiming, provincial teacher of the smokeing panel test two.Smoking result shows, beet medicinal extract has the delicate fragrance note of beet, and with pleasant sweet and pure fragrance; Add in cigarette, it can increase delicate fragrance note and soft flue gas, reduces the pungency of cigarette, can use as Novel tobacco flavor.
According to evaluation method of smokeing panel test equally, cigarette garden beet medicinal extract prepared by the beet medicinal extract obtained after being concentrated by the sugar beet juice of squeezing and the inventive method contrasts.Cigarette garden beet medicinal extract without plant lactobacillus process is compared with the inventive method, and only lacked plant lactobacillus treatment step, other are identical.The results are shown in following table.
| Sample ID | Cigarette evaluation |
| Without the cigarette garden beet medicinal extract of plant lactobacillus process | Reduce stimulation, soft flue gas, lingual surface has residual |
| Cigarette garden beet medicinal extract prepared by the inventive method | Increase delicate fragrance note, reduce stimulation, soft flue gas |
Claims (6)
1. plant lactobacillus process sugar beet juice prepares a method for fermented type beet medicinal extract, it is characterized in that, comprises the following steps:
(1) preparation of sugar beet juice:
Fresh beet is added in clear water and cleans up, then carry out making beating process, collect slurries, obtain beet slurries; In gained beet slurries, add the Nacl that the glucose that weight is beet slurry weight 0.1% ~ 5% and weight are beet slurry weight 0.01% ~ 0.5%, mix rear sterilizing, obtain sugar beet juice;
(2) preparation of plant lactobacillus bacterium liquid:
Plant lactobacillus LactobacillusplantarumPJH05 is added to the water preparation bacterium liquid, obtains plant lactobacillus bacterium liquid; Described plant lactobacillus LactobacillusplantarumPJH05 is deposited in the China typical culture collection center CCTCC being positioned at Wuhan University of Wuhan City of Hubei China province, and preserving number is: CCTCCNO:M2012002;
(3) ferment:
Be 0.1 ~ 1% by the mass ratio of plant lactobacillus bacterium liquid and sugar beet juice, gained plant lactobacillus bacterium liquid be inoculated in the sugar beet juice after sterilizing, obtain postvaccinal sugar beet juice; Postvaccinal for gained sugar beet juice is fermented, obtains beet fermented liquid;
(4) filtering separation:
Gained beet fermented liquid is carried out filtering separation, obtains the beet filtrate of clarifying;
(5) concentrating under reduced pressure:
Gained beet filtrate is carried out concentrating under reduced pressure, and obtaining relative density is 1.150 ~ 1.350g/cm
3fermented type beet medicinal extract.
2. a kind of plant lactobacillus process sugar beet juice according to claim 1 prepares the method for fermented type beet medicinal extract, it is characterized in that: the sterilizing in described step (1) is that sugar beet juice is carried out sterilising treatment 15 ~ 30min at 100 ~ 115 DEG C.
3. a kind of plant lactobacillus process sugar beet juice according to claim 1 prepares the method for fermented type beet medicinal extract, it is characterized in that: the water in described step (2) is the sterilized water of 100 times of quality.
4. a kind of plant lactobacillus process sugar beet juice according to claim 1 prepares the method for fermented type beet medicinal extract, it is characterized in that: the fermentation in described step (3) is first 2.5 ~ 5.5 in pH value by postvaccinal for gained sugar beet juice, mixing speed is 200 ~ 500rpm, air flow is 1 ~ 3VVM, and leavening temperature is the condition bottom fermentation 1d ~ 7d of 15 DEG C ~ 30 DEG C; Then be the condition bottom fermentation 7d ~ 28d of 5 DEG C ~ 15 DEG C at leavening temperature.
5. a kind of plant lactobacillus process sugar beet juice according to claim 1 prepares the method for fermented type beet medicinal extract, it is characterized in that: the filtering separation in described step (4) is that gained beet fermented liquid is first used filtered through gauze, more again filters with the filter membrane of 0.8 micron.
6. a kind of plant lactobacillus process sugar beet juice according to claim 1 prepares the method for fermented type beet medicinal extract, it is characterized in that: the concentrating under reduced pressure temperature in described step (5) is 50 ~ 80 DEG C, and concentrated pressure is 0.01 ~ 0.2 normal atmosphere.
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| CN106497906A (en) * | 2016-10-13 | 2017-03-15 | 湖北中烟工业有限责任公司 | A kind of preparation method of cigarette chamomile extract |
| CN106957837A (en) * | 2016-10-13 | 2017-07-18 | 武汉黄鹤楼香精香料有限公司 | A kind of method that fixing lactic acid bacteria prepares cigarette Semen Persicae extract |
| CN106957872A (en) * | 2016-10-13 | 2017-07-18 | 武汉黄鹤楼香精香料有限公司 | A kind of method that fixing lactic acid bacteria prepares cigarette Semen Armeniacae Amarum extract |
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| CN108244425A (en) * | 2016-12-29 | 2018-07-06 | 中粮集团有限公司 | A kind of juice functional beverage and preparation method thereof |
| CN107041567A (en) * | 2017-02-15 | 2017-08-15 | 广西中烟工业有限责任公司 | A kind of lemon fermented type tobacco aromaticss and its preparation method and application |
| CN110801023A (en) * | 2019-12-12 | 2020-02-18 | 山西达明一派食品有限公司 | Method for fermenting beet root pulp by using plant probiotics |
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