CN108142984A - A kind of method for preparing tobacco leaf clout fragrance using the golden flower bacterium fermentation of tea source - Google Patents
A kind of method for preparing tobacco leaf clout fragrance using the golden flower bacterium fermentation of tea source Download PDFInfo
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- CN108142984A CN108142984A CN201711317916.XA CN201711317916A CN108142984A CN 108142984 A CN108142984 A CN 108142984A CN 201711317916 A CN201711317916 A CN 201711317916A CN 108142984 A CN108142984 A CN 108142984A
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- 238000000855 fermentation Methods 0.000 title claims abstract description 18
- 230000004151 fermentation Effects 0.000 title claims abstract description 18
- 244000269722 Thea sinensis Species 0.000 title claims abstract 12
- 244000061176 Nicotiana tabacum Species 0.000 title 1
- 241000208125 Nicotiana Species 0.000 claims abstract description 87
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- 238000000605 extraction Methods 0.000 claims description 8
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- 238000005516 engineering process Methods 0.000 claims description 6
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 4
- 239000001888 Peptone Substances 0.000 claims description 3
- 108010080698 Peptones Proteins 0.000 claims description 3
- 229940041514 candida albicans extract Drugs 0.000 claims description 3
- 238000010790 dilution Methods 0.000 claims description 3
- 239000012895 dilution Substances 0.000 claims description 3
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- 238000012216 screening Methods 0.000 claims description 3
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- 238000003756 stirring Methods 0.000 claims description 2
- 235000019504 cigarettes Nutrition 0.000 abstract description 15
- UGFAIRIUMAVXCW-UHFFFAOYSA-N Carbon monoxide Chemical compound [O+]#[C-] UGFAIRIUMAVXCW-UHFFFAOYSA-N 0.000 abstract description 7
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- 238000002360 preparation method Methods 0.000 description 3
- GLZPCOQZEFWAFX-UHFFFAOYSA-N Geraniol Chemical compound CC(C)=CCCC(C)=CCO GLZPCOQZEFWAFX-UHFFFAOYSA-N 0.000 description 2
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- 239000005792 Geraniol Substances 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical group [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
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- 150000001298 alcohols Chemical class 0.000 description 1
- 150000001299 aldehydes Chemical class 0.000 description 1
- GZCGUPFRVQAUEE-SLPGGIOYSA-N aldehydo-D-glucose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C=O GZCGUPFRVQAUEE-SLPGGIOYSA-N 0.000 description 1
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- 229940113087 geraniol Drugs 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
- A24B15/18—Treatment of tobacco products or tobacco substitutes
- A24B15/24—Treatment of tobacco products or tobacco substitutes by extraction; Tobacco extracts
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- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Manufacture Of Tobacco Products (AREA)
Abstract
The present invention provides a kind of method for preparing tobacco leaf clout fragrance using the golden flower bacterium fermentation of tea source.The tobacco spice is to utilize the tea source aroma former golden flower bacterium screened from tealeaves for the first time, tobacco leaf clout is fermented after ultrasonic wave tentatively extracts by golden flower bacterium, tobacco leaf clout extract is prepared into, obtains the first fragrance by reduced pressure, alcohol precipitation, centrifugation, concentration successively again；Then tobacco leaf clout extract is subjected to molecular distillation processing, obtains molecular distillation light component, and in cigarette.The present invention for the first time ferments tea source aroma former golden flower bacterium applied to tobacco extract, once obtains two kinds of fragrance, while solve the problems, such as the waste of a large amount of tobacco leaf clouts, promotes leaf tobacco extract quality, and application is strong；Meanwhile obtained fragrance can be obviously improved cigarette flavor matter, while also have soft fine and smooth flue gas, increase the effect of the sweet sense in oral cavity and improvement the flue gas property sent out thoroughly.
Description
Technical field
It is specifically a kind of to be prepared using the golden flower bacterium fermentation of tea source the present invention relates to a kind of preparation method of tobacco aromaticss
The method of tobacco leaf clout fragrance.
Background technology
Tobacco leaf is Solanaceae Nicotiana plant, originates in South America, is introduced a fine variety in China relatively early, yield is big.At present, China tobacco
Cultivated area and yield of tobacco occupy first place in the world, and the related income for being related to tobacco business is sent out in the national economic development in China
Wave important role.During the plantation of tobacco, harvesting, transport, storage and tobacco leaf processing etc., there are a large amount of tobacco leaf clouts
It generates, accounts for about 20% or so of tobacco leaf total output, due to the particularity of its contained chemical composition, if dealing with improperly, not only waste
Resource, also environment easy to pollute.
Containing abundant aroma substance in tealeaves, so far, the tea aroma substance of separated identification there are about 700 kinds,
The composition of tea aroma substance includes hydrocarbon (14.22%), alcohols (12.76%), aldehydes (10.30%), ketone
(15.35%), esters and lactone (12.44%), compound containing N (13.41%), acids, phenols, miscellaneous oxygen compound, sulfur-bearing
Compounds etc..But its main component is only tens of kinds, such as geraniol, cis- 3- enol, linalool and its oxide, benzene first
Alcohol etc..Beneficial producing fragrant strains are screened from tealeaves, are a good choice directions.
The bacterial strain that fermented type tobacco aromaticss used in the prior art use be mostly industrialize bacterial strain, and its to ferment bottom
The specific aim of object is not strong, and utilizes the tea source golden flower bacterium fermenting and producing characteristic tobacco aromaticss independently screened by raw material of tobacco leaf clout
Method have not been reported.
Invention content
The present invention is exactly to overcome deficiency present in above-mentioned technology of preparing, provides a kind of preparation side of tobacco leaf clout fragrance
Method, the method is not only efficiently quick, energy conservation and environmental protection, but also for the first time sends out tea source aroma former golden flower bacterium applied to tobacco extract
Ferment once obtains two kinds of fragrance, while solves the problems, such as the waste of a large amount of tobacco leaf clouts, promotes leaf tobacco extract quality, application
By force；Meanwhile obtained fragrance can be obviously improved cigarette flavor matter, while also have the function of soft fine and smooth flue gas, roll up
There is ideal application effect in cigarette.
Technical solution provided by the invention：A kind of method for preparing tobacco leaf clout fragrance using the golden flower bacterium fermentation of tea source,
It is characterized in that being as follows：
(1) the coronoid process dissipate capsule bacterium strain screened from tealeaves is positioned at 20~40 DEG C of temperature 1~4 hour, then will
It is inoculated on fluid nutrient medium by the inoculum concentration of culture medium weight 1~5%, it is small that 12~48 is cultivated under conditions of 20~40 DEG C
When, golden flower bacterium bacteria suspension is prepared；
(2) tobacco leaf clout fragment is weighed, 8-10 times of water is added in and impregnates 3-5h, then in supersonic frequency 50-70KHz, temperature
Ultrasonic wave extraction 2-3h under conditions of 40 DEG C -60 DEG C of degree is filtered after extracting solution standing is cooled to room temperature, and is collected filtrate and is obtained cigarette
Leaf clout extracting solution；
(3) the golden flower bacterium bacteria suspension in step (1) is added to according to the 0.1%-0.2% of tobacco leaf clout extracting solution weight
The tobacco leaf clout extracting solution collected in step (2), and the glucose of the 0.5-1.5% of tobacco leaf clout extracting solution weight is added in,
It ferments 4-8 days under the conditions of 24-30 DEG C, obtains tobacco leaf clout zymotic fluid；
(4) by tobacco leaf clout zymotic fluid in step (3) in the condition that temperature is 60-80 DEG C, pressure is 0.01-0.02MPa
Lower reduced pressure obtains the concentrate that relative density is 1.1-1.3；
(5) 95% ethanol solution of concentration of 1-3 times of its weight is added in the concentrate into step (4), stirs 15-
30min is stood the ethyl alcohol that 24-48h is obtained and stands liquid, then filtered using centrifuge at ambient temperature, and filtrate decompression is dense
It is 1.15~1.25 to be reduced to relative density, and tobacco leaf clout extract i.e. fragrance A is prepared；
(6) propylene glycol that its weight 10%~30% is added in the tobacco leaf clout extract prepared in step (5), mixing are taken
After uniformly, molecular distillation pretreatment liquid is obtained；
(7) the molecular distillation pretreatment liquid obtained in step (6) is subjected to molecular distillation separation, separating technology parameter is：
Vacuum degree≤2.0tor, scraper plate rotating speed to 30-60r/min, 30-60 DEG C of vapo(u)rizing temperature, condensation temperature -12~0 DEG C, distillation speed
1.1-4.0Kg/h obtains molecular distillation light component, as fragrance B.
The present invention preferably technical solution：The screening step of golden flower bacterium is in the step (1)：With yiyang, hunan tea processing factory Fu
Brick tea is raw material, and with dilution, flat band method detaches the coronoid process dissipate capsule bacterium (golden flower bacterium) on Fu-brick tea, is inoculated in CZG cultures
Culture is inverted on base under 30 DEG C of constant temperature, after repeatedly purifying culture, obtains colony colour and the more consistent bacterium colony of form ratio,
Then the single bacterium colony of picking is purified on CZG tablets, obtains the Pure cultured spawn of coronoid process dissipate capsule bacterium (golden flower bacterium).
The present invention preferably technical solution：In the step (4) addition of glucose be 1%, fermentation temperature be
25 DEG C, fermentation time is 5 days.
The present invention preferably technical solution：Step (5) the tobacco leaf clout zymotic fluid is temperature is 70 DEG C, pressure is
It is concentrated under reduced pressure under the conditions of 0.01MPa, obtains the concentrate that relative density is 1.1.
The present invention preferably technical solution：Liquid Culture based formulas in the step (1) is：5 grams of peptone, glucose
40 grams, 2.5 grams of yeast extract.
Beneficial effects of the present invention：
(1) present invention solves the problems, such as the waste of a large amount of tobacco leaf clouts, is effectively utilized waste material；
(2) present invention utilizes the tea source aroma former golden flower bacterium screened from tealeaves for the first time, and tobacco leaf clout is passed through ultrasonic wave
It is fermented after preliminary extraction by golden flower bacterium, increases and improve the quality of tobacco leaf clout fragrance product to varying degrees, improved
Cigarette quality；
(3) for tobacco leaf clout extracting solution of the invention using ultrasonic extracting process, extraction process extraction is abundant, does not destroy
Active ingredient, and have the advantages that easily operated, post processing is simple, it is converted conducive to production；Overcome common solvent refluxing extraction
The residual that obtained extract comes to tobacco rolling belt, can obviously reduce stimulation, oral cavity pleasant impression is clean；
(4) present invention concentration after ethyl alcohol stand liquid using centrifuge filtering, can remove macro-molecular protein in filtrate,
Macromolecular cellulose and macromolecular polysaccharide, by there was only small-molecule substance in filtered fluid, volatility is more preferable；And it uses
Centrifuge filters, and filter efficiency is high, and effect is good, simple and convenient, economical and energy saving；
(5) present invention can once obtain two kinds of fragrance, energy-efficient, and utilization rate is high.
The method of the present invention is efficiently quick, energy conservation and environmental protection, and tea source aroma former golden flower bacterium is applied to tobacco for the first time
Class extract ferments, and once obtains two kinds of fragrance, while solve the problems, such as the waste of a large amount of tobacco leaf clouts, promotes leaf tobacco extract product
Matter, application are strong；Meanwhile obtained fragrance can be obviously improved cigarette flavor matter, while also have soft fine and smooth flue gas, increase
Add the sweet sense in oral cavity and the effect of the improvement flue gas property sent out thoroughly.
Specific embodiment
With reference to embodiment, the invention will be further described.
The preparation method of golden flower bacterium bacteria suspension is as follows in following embodiment：Using yiyang, hunan tea processing factory Fu-brick tea as raw material, use
Flat band method detaches the golden flower bacterium (coronoid process dissipate capsule bacterium) on Fu-brick tea for dilution, is inoculated on CZG culture mediums in 30 DEG C of perseverances
Temperature is lower to be inverted culture, after repeatedly purifying culture, obtains colony colour and the more consistent bacterium colony of form ratio, then picking is single
Bacterium colony is purified on CZG tablets, obtains the Pure cultured spawn of golden flower bacterium；Then by tea source golden flower bacterium strain be positioned over temperature 20~
1~4 hour at 40 DEG C, then it will be inoculated on liquid activation medium by the inoculum concentration of culture medium weight 1~5%, 20~
It is cultivated 12~48 hours under conditions of 40 DEG C, golden flower bacterium bacteria suspension is prepared.The Liquid Culture based formulas is：Peptone 5
Gram, 40 grams of glucose, 2.5 grams of yeast extract.
A kind of embodiment 1, method for preparing tobacco leaf clout fragrance using the golden flower bacterium fermentation of tea source, is as follows：
(1) 1000g tobacco leaf clout fragments are weighed, the water for adding in 8000mL impregnates, and carries out ultrasonic wave extraction 1.5h, will extract
Liquid stands cooled and filtered, collects filtrate and obtains tobacco leaf clout extracting solution；The supersonic frequency 60KHz, temperature 50 C；
(2) the tobacco leaf clout extracting solution being added to the above-mentioned golden flower bacterium bacteria suspension of 8ml in step (1), and add in tobacco leaf
The glucose of clout extracting solution weight 1% ferments 5 days under the conditions of 25 DEG C, obtains tobacco leaf clout zymotic fluid；
(3) tobacco leaf clout zymotic fluid in step (2) is concentrated under reduced pressure under the conditions of temperature is 70 DEG C, pressure is 0.01MPa,
Obtain the concentrate that relative density is 1.1；
(4) 95% ethanol solution of concentration of 2 times of its weight is added in concentrate into step (3), 20min is stirred, in room
48h is stood under the conditions of temperature；
(5) ethyl alcohol for obtaining step (4) stands liquid, is filtered using centrifuge, concentrates filtrate to relative density
It is 1.15, tobacco leaf clout extract is prepared, obtains fragrance A；
(6) propylene glycol of its weight 20% is added in the tobacco leaf clout extract prepared into step (5), is uniformly mixed
Afterwards, molecular distillation pretreatment liquid is obtained；
(7) molecular distillation pretreatment liquid will be obtained in step (6) and carries out molecular distillation separation, separating technology parameter is：Very
Reciprocal of duty cycle≤2.0tor, scraper plate rotating speed to 30r/min, 60 DEG C of vapo(u)rizing temperature, 0 DEG C, distillation speed 1.1-4.0Kg/h of condensation temperature,
Molecular distillation light component is obtained to get to fragrance B.
A kind of embodiment 2, method for preparing tobacco leaf clout fragrance using the golden flower bacterium fermentation of tea source, is as follows：
(1) 1000g tobacco leaf clout fragments are weighed, the water for adding in 8000mL impregnates, and ultrasonic wave extraction 2h is carried out, by extracting solution
Cooled and filtered is stood, filtrate is collected and obtains tobacco leaf clout extracting solution；The supersonic frequency 50KHz, temperature 50 C；
(2) the tobacco leaf clout extracting solution being added to 8ml above-mentioned golden flower bacterium bacteria suspensions in step (1) adds in 1% grape
Sugar ferments 5 days under the conditions of 25 DEG C, obtains tobacco leaf clout zymotic fluid；
(3) tobacco leaf clout zymotic fluid in step (2) is concentrated under reduced pressure under the conditions of temperature is 70 DEG C, pressure is 0.01MPa,
Obtain the concentrate that relative density is 1.1；
(4) 95% ethanol solution of concentration of 2 times of its weight is added in concentrate into step (3), 20min is stirred, in room
36h is stood under the conditions of temperature；
(5) ethyl alcohol for obtaining step (4) stands liquid, is filtered using centrifuge, concentrates filtrate to relative density
It is 1.20, tobacco leaf clout extract is prepared, obtains fragrance A；
(6) propylene glycol of its weight 30% is added in the tobacco leaf clout extract prepared into step (5), is uniformly mixed
Afterwards, molecular distillation pretreatment liquid is obtained；
(7) molecular distillation pretreatment liquid will be obtained in step (6) and carries out molecular distillation separation, separating technology parameter is：Very
Reciprocal of duty cycle≤2.0tor, scraper plate rotating speed to 30r/min, 50 DEG C of vapo(u)rizing temperature, -5 DEG C of condensation temperature, distillation speed 1.1-4.0Kg/h,
Molecular distillation light component is obtained to get to fragrance B.
Contrast test：It is directly sprayed after the fragrance A prepared in the present invention and fragrance B is diluted using a concentration of 70% ethyl alcohol
It is spread across on pipe tobacco and flavoring is carried out to pipe tobacco, the additive amount of fragrance is that the ratio of pipe tobacco weight is 5 ‰, then by the pipe tobacco after flavoring
Cigarette is made, the professional cigarette personnel that smoke panel test is allowed to smoke panel test；Made simultaneously using tobacco leaf clout fragrance prepared by normal fermentation technique
It is to be sprayed onto on pipe tobacco to carry out flavoring to pipe tobacco after blank sample is diluted using a concentration of 70% ethyl alcohol, the additive amount of blank sample is
The ratio of pipe tobacco weight is 5 ‰, and the pipe tobacco after flavoring is made cigarette, and the professional cigarette personnel that smoke panel test is allowed to smoke panel test；And by three
The smoking result of kind cigarette is compared, table specific as follows：
| Title | Aroma quality | Perfume quantity | It is rich | Exquisiteness | Strength | Concentration | Miscellaneous gas | Irritation | Dry sensation | Pleasant impression | Total score |
| Blank sample | 7 | 6 | 7 | 6 | 7 | 6 | 7 | 6 | 7 | 7 | 66 |
| Fragrance A | 8 | 8 | 8 | 8 | 7 | 8 | 8 | 7 | 8 | 7 | 77 |
| Fragrance B | 9 | 8 | 8 | 9 | 7 | 9 | 9 | 9 | 8 | 8 | 84 |
As seen from the above table, tobacco leaf clout after ultrasonic wave tentatively extracts by the golden flower bacterium of laboratory screening is fermented and made
The fragrance A obtained is increased substantially than the quality of fragrance is made in normal fermentation, and obtained fragrance A can be obviously improved cigarette perfume
Makings, while also have soft fine and smooth flue gas increases the effect of the sweet sense in oral cavity and improvement the flue gas property sent out thoroughly.Meanwhile by dividing
The fragrance B that son distillation is further processed, can further promote cigarette quality, can obviously reduce stimulation, and oral cavity pleasant impression is clean,
There is better application in cigarette.
Claims (5)
- A kind of 1. method for preparing tobacco leaf clout fragrance using the golden flower bacterium fermentation of tea source, it is characterised in that be as follows：(1) the coronoid process dissipate capsule bacterium strain screened from tealeaves is positioned at 20~40 DEG C of temperature 1~4 hour, it then will be by training The inoculum concentration for supporting base weight amount 1~5% is inoculated on fluid nutrient medium, is cultivated 12~48 hours under conditions of 20~40 DEG C, is made It is standby to obtain golden flower bacterium bacteria suspension；(2) tobacco leaf clout fragment is weighed, 8-10 times of water is added in and impregnates 3-5h, then in supersonic frequency 50-70KHz, temperature 40 Ultrasonic wave extraction 2-3h under conditions of DEG C -60 DEG C is filtered after extracting solution standing is cooled to room temperature, and is collected filtrate and is obtained more than tobacco leaf Expect extracting solution；(3) the golden flower bacterium bacteria suspension in step (1) is added to step according to the 0.1%-0.2% of tobacco leaf clout extracting solution weight (2) the tobacco leaf clout extracting solution collected in, and the glucose of the 0.5-1.5% of tobacco leaf clout extracting solution weight is added in, in 24-30 It ferments 4-8 days under the conditions of DEG C, obtains tobacco leaf clout zymotic fluid；(4) tobacco leaf clout zymotic fluid in step (3) is subtracted under conditions of temperature is 60-80 DEG C, pressure is 0.01-0.02MPa Pressure concentration obtains the concentrate that relative density is 1.1-1.3；(5) 95% ethanol solution of concentration of 1-3 times of its weight is added in the concentrate into step (4), stirs 15-30min, The ethyl alcohol that 24-48h is obtained is stood under room temperature and stands liquid, is then filtered using centrifuge, is concentrated filtrate to opposite Density is 1.15~1.25, and tobacco leaf clout extract i.e. fragrance A is prepared；(6) propylene glycol that its weight 10%~30% is added in the tobacco leaf clout extract prepared in step (5) is taken, is uniformly mixed Afterwards, molecular distillation pretreatment liquid is obtained；(7) the molecular distillation pretreatment liquid obtained in step (6) is subjected to molecular distillation separation, separating technology parameter is：Vacuum Degree≤2.0tor, scraper plate rotating speed to 30-60r/min, 30-60 DEG C of vapo(u)rizing temperature, condensation temperature -12~0 DEG C, distillation speed 1.1- 4.0Kg/h obtains molecular distillation light component, as fragrance B.
- 2. a kind of method for preparing tobacco leaf clout fragrance using the golden flower bacterium fermentation of tea source according to claim 1, feature The screening step for being golden flower bacterium in the step (1) is：Using yiyang, hunan tea processing factory Fu-brick tea as raw material, with dilution fall flat band method Coronoid process dissipate capsule bacterium on Fu-brick tea is detached, is inoculated on CZG culture mediums under 30 DEG C of constant temperature and is inverted culture, by multiple After purifying culture, colony colour and the more consistent bacterium colony of form ratio are obtained, then the single bacterium colony of picking is purified on CZG tablets, Obtain the Pure cultured spawn of coronoid process dissipate capsule bacterium.
- 3. a kind of method for preparing tobacco leaf clout fragrance using the golden flower bacterium fermentation of tea source according to claim 1, feature It is：The addition of glucose is 1% in the step (4), and fermentation temperature is at 25 DEG C, and fermentation time is 5 days.
- 4. a kind of method for preparing tobacco leaf clout fragrance using the golden flower bacterium fermentation of tea source according to claim 1, feature It is：Step (5) the tobacco leaf clout zymotic fluid is concentrated under reduced pressure under the conditions of temperature is 70 DEG C, pressure is 0.01MPa, obtains phase To the concentrate that density is 1.1.
- 5. a kind of method for preparing tobacco leaf clout fragrance using the golden flower bacterium fermentation of tea source according to claim 1, feature The formula for being the fluid nutrient medium in the step (1) is：5 grams of peptone, 40 grams of glucose, 2.5 grams of yeast extract.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711317916.XA CN108142984B (en) | 2017-12-12 | 2017-12-12 | Method for preparing tobacco leaf residue spice by fermenting tea-source golden florets |
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| CN112442421A (en) * | 2020-11-19 | 2021-03-05 | 河南中烟工业有限责任公司 | Preparation method of camphor-type tobacco flavor |
| CN113564059A (en) * | 2021-07-15 | 2021-10-29 | 四川中烟工业有限责任公司 | Abnormal Wickhamella strain and application thereof |
| CN114958486A (en) * | 2022-06-20 | 2022-08-30 | 甘肃烟草工业有限责任公司 | Rhodiola rosea tobacco flavor and preparation method and application thereof |
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