CN105852189B - A kind of method that utilization waste/hypo-tobacco leaf prepares tobacco extract - Google Patents
A kind of method that utilization waste/hypo-tobacco leaf prepares tobacco extract Download PDFInfo
- Publication number
- CN105852189B CN105852189B CN201610375545.XA CN201610375545A CN105852189B CN 105852189 B CN105852189 B CN 105852189B CN 201610375545 A CN201610375545 A CN 201610375545A CN 105852189 B CN105852189 B CN 105852189B
- Authority
- CN
- China
- Prior art keywords
- tobacco
- hypo
- extract
- tobacco leaf
- waste
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 235000002637 Nicotiana tabacum Nutrition 0.000 title claims abstract description 90
- 241000208125 Nicotiana Species 0.000 title claims abstract description 89
- 239000000284 extract Substances 0.000 title claims abstract description 84
- 239000002699 waste material Substances 0.000 title claims abstract description 63
- 238000000034 method Methods 0.000 title claims abstract description 28
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 63
- 238000000199 molecular distillation Methods 0.000 claims abstract description 33
- 230000000694 effects Effects 0.000 claims abstract description 21
- 239000000919 ceramic Substances 0.000 claims abstract description 14
- 239000012528 membrane Substances 0.000 claims abstract description 14
- 235000019504 cigarettes Nutrition 0.000 claims abstract description 13
- 239000012634 fragment Substances 0.000 claims abstract description 11
- 238000003756 stirring Methods 0.000 claims abstract description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 11
- 102000005158 Subtilisins Human genes 0.000 claims abstract description 10
- 108010056079 Subtilisins Proteins 0.000 claims abstract description 10
- 239000000796 flavoring agent Substances 0.000 claims abstract description 9
- 235000019634 flavors Nutrition 0.000 claims abstract description 9
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract description 8
- 239000008103 glucose Substances 0.000 claims abstract description 8
- 102000004190 Enzymes Human genes 0.000 claims abstract description 5
- 108090000790 Enzymes Proteins 0.000 claims abstract description 5
- 239000007788 liquid Substances 0.000 claims description 35
- 239000000243 solution Substances 0.000 claims description 23
- 239000000287 crude extract Substances 0.000 claims description 21
- 239000002994 raw material Substances 0.000 claims description 19
- 108010059892 Cellulase Proteins 0.000 claims description 8
- 229940106157 cellulase Drugs 0.000 claims description 8
- 238000004821 distillation Methods 0.000 claims description 8
- 238000000926 separation method Methods 0.000 claims description 8
- 239000007864 aqueous solution Substances 0.000 claims description 7
- 238000009833 condensation Methods 0.000 claims description 7
- 230000005494 condensation Effects 0.000 claims description 7
- 238000005374 membrane filtration Methods 0.000 claims description 7
- ULWHHBHJGPPBCO-UHFFFAOYSA-N propane-1,1-diol Chemical class CCC(O)O ULWHHBHJGPPBCO-UHFFFAOYSA-N 0.000 claims description 7
- 229940088598 enzyme Drugs 0.000 claims description 4
- 239000000835 fiber Substances 0.000 claims description 3
- 239000003205 fragrance Substances 0.000 abstract description 6
- UGFAIRIUMAVXCW-UHFFFAOYSA-N Carbon monoxide Chemical compound [O+]#[C-] UGFAIRIUMAVXCW-UHFFFAOYSA-N 0.000 abstract description 5
- 239000003546 flue gas Substances 0.000 abstract description 5
- 239000000203 mixture Substances 0.000 abstract description 4
- 235000013599 spices Nutrition 0.000 abstract description 4
- 235000009508 confectionery Nutrition 0.000 abstract description 3
- 210000000214 mouth Anatomy 0.000 abstract description 3
- 229920003266 Leaf® Polymers 0.000 description 69
- 238000001914 filtration Methods 0.000 description 11
- 239000000047 product Substances 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 238000001556 precipitation Methods 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 244000025254 Cannabis sativa Species 0.000 description 2
- 244000061176 Nicotiana tabacum Species 0.000 description 2
- 229920002678 cellulose Polymers 0.000 description 2
- 239000001913 cellulose Substances 0.000 description 2
- 239000006071 cream Substances 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 239000000706 filtrate Substances 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 150000004676 glycans Chemical class 0.000 description 2
- 238000002386 leaching Methods 0.000 description 2
- 229920001282 polysaccharide Polymers 0.000 description 2
- 239000005017 polysaccharide Substances 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 238000004064 recycling Methods 0.000 description 2
- 230000001953 sensory effect Effects 0.000 description 2
- SNICXCGAKADSCV-JTQLQIEISA-N (-)-Nicotine Chemical compound CN1CCC[C@H]1C1=CC=CN=C1 SNICXCGAKADSCV-JTQLQIEISA-N 0.000 description 1
- CWVRJTMFETXNAD-FWCWNIRPSA-N 3-O-Caffeoylquinic acid Natural products O[C@H]1[C@@H](O)C[C@@](O)(C(O)=O)C[C@H]1OC(=O)\C=C\C1=CC=C(O)C(O)=C1 CWVRJTMFETXNAD-FWCWNIRPSA-N 0.000 description 1
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- PZIRUHCJZBGLDY-UHFFFAOYSA-N Caffeoylquinic acid Natural products CC(CCC(=O)C(C)C1C(=O)CC2C3CC(O)C4CC(O)CCC4(C)C3CCC12C)C(=O)O PZIRUHCJZBGLDY-UHFFFAOYSA-N 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 244000283207 Indigofera tinctoria Species 0.000 description 1
- CWVRJTMFETXNAD-KLZCAUPSSA-N Neochlorogenin-saeure Natural products O[C@H]1C[C@@](O)(C[C@@H](OC(=O)C=Cc2ccc(O)c(O)c2)[C@@H]1O)C(=O)O CWVRJTMFETXNAD-KLZCAUPSSA-N 0.000 description 1
- 102000035195 Peptidases Human genes 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 101000693530 Staphylococcus aureus Staphylokinase Proteins 0.000 description 1
- 230000001476 alcoholic effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- CWVRJTMFETXNAD-JUHZACGLSA-N chlorogenic acid Chemical compound O[C@@H]1[C@H](O)C[C@@](O)(C(O)=O)C[C@H]1OC(=O)\C=C\C1=CC=C(O)C(O)=C1 CWVRJTMFETXNAD-JUHZACGLSA-N 0.000 description 1
- 229940074393 chlorogenic acid Drugs 0.000 description 1
- FFQSDFBBSXGVKF-KHSQJDLVSA-N chlorogenic acid Natural products O[C@@H]1C[C@](O)(C[C@@H](CC(=O)C=Cc2ccc(O)c(O)c2)[C@@H]1O)C(=O)O FFQSDFBBSXGVKF-KHSQJDLVSA-N 0.000 description 1
- 235000001368 chlorogenic acid Nutrition 0.000 description 1
- BMRSEYFENKXDIS-KLZCAUPSSA-N cis-3-O-p-coumaroylquinic acid Natural products O[C@H]1C[C@@](O)(C[C@@H](OC(=O)C=Cc2ccc(O)cc2)[C@@H]1O)C(=O)O BMRSEYFENKXDIS-KLZCAUPSSA-N 0.000 description 1
- 238000005352 clarification Methods 0.000 description 1
- 239000011365 complex material Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000012043 crude product Substances 0.000 description 1
- 230000001186 cumulative effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- -1 iginates Species 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 229960002715 nicotine Drugs 0.000 description 1
- SNICXCGAKADSCV-UHFFFAOYSA-N nicotine Natural products CN1CCCC1C1=CC=CN=C1 SNICXCGAKADSCV-UHFFFAOYSA-N 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 235000019419 proteases Nutrition 0.000 description 1
- 238000010992 reflux Methods 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- 239000000779 smoke Substances 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B3/00—Preparing tobacco in the factory
- A24B3/14—Forming reconstituted tobacco products, e.g. wrapper materials, sheets, imitation leaves, rods, cakes; Forms of such products
Landscapes
- Manufacture Of Tobacco Products (AREA)
- Vaporization, Distillation, Condensation, Sublimation, And Cold Traps (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The present invention provides a kind of method that utilization waste/hypo-tobacco leaf prepares tobacco extract.The tobacco extract be will between volume hired car offal, tobacco leaf fragment, offal mix add ethanol solution to extract, it is concentrated under reduced pressure; sequentially add pure water, glucose; ethanol solution is added to stand after its weight fibres element enzyme and subtilopeptidase A enzymolysis are sequentially added after stirring; ceramic membrane filter is used again, and it is isolated to carry out molecular distillation after being concentrated under reduced pressure.Efficiently quick, energy-conserving and environment-protective of the invention, and obtained molecular distillation thing fragrance is true to nature, improves mouthfeel obvious, used as essence spice for cigarette, cigarette flavor matter can be obviously improved, while also having soft fine and smooth flue gas, be increased the sweet sense in oral cavity and is improved the effect of the flue gas property sent out thoroughly.
Description
Technical field
It is specifically a kind of to prepare tobacco leaching using waste/hypo-tobacco leaf the present invention relates to a kind of preparation method of tobacco extract
The method of cream.
Technical background
Tobacco, is Solanaceae Nicotiana plant, originates in South America, introduces a fine variety relatively early in China, and yield is big.At present, China tobacco
Cultivated area and yield of tobacco occupy first place in the world, and are related to the related income of tobacco business to be sent out in the national economic development of China
Wave important effect.During plantation, harvesting, transport, storage and tobacco leaf processing in tobacco etc., there are a large amount of waste/hypo-tobacco leafs
Produce, account for the 25%-30% of tobacco leaf total output, due to the particularity of its contained chemical composition, if dealing with improperly, not only waste
Resource, also easily pollutes environment.Knowable to current document report, experts and scholars are had with waste/hypo-tobacco leaf as raw material, using difference
Technical method, extract the compositions such as Salanesol therein, nicotine, chlorogenic acid, and using waste/hypo-tobacco leaf prepare fertilizer, feed and
Biological pesticide etc., but above method technique is complex, and raw material availability is not high, easily causes environmental pollution.
Also have at present and many patents disclose some by waste/hypo-tobacco leaf for the method for preparing tobacco extract, such as patent No.
For：The method that a kind of tobacco waste disclosed in CN201210412335.5 prepares tobacco extract, the method is directly by discarded cigarette
Add water boiling after the crushing of end, is then concentrated with redistillation after compound ferment treatment；Patent No.：Disclosed in CN201210059368.6
In a kind of tobacco extract and its extracting method and application, the tobacco extract in the patent is added to by tobacco cane bast powder
Ethanol refilters addition neutral proteinase extraction after extracting after, reuse ethanol and distilled as extractant；The patent No.
For：A kind of preparation method of tobacco extract disclosed in CN200710032351.0, the method is that discarded offal adds water to enter with enzyme
Row enzymolysis, is subsequently adding alcohol reflux extraction, using the nervous evaporation of film evaporator after filtering and concentrating, then by U.S. after concentrating
Maillard reaction obtains tobacco extract；The patent No.：A kind of utilization biology enzyme prepares tobacco extract disclosed in CN2005100295668
Method, the method is to use offal, discarded tobacco leaf and tobacco leaf to be digested by being added water after crushing for raw material, to enzymolysis
Filtered fluid carries out concentration and obtains.Although the above method can also prepare tobacco extract, deposited in the tobacco extract for preparing
In many macro-molecular proteins, macromolecular fiber and macromolecular polysaccharide, these materials should not volatilize, be deposited in medicinal extract, influence
The volatility of medicinal extract spices, reduces the mildness and fine and smooth degree of spices fragrance, causes the tobacco extract for preparing in fragrance
Fidelity, cigarette effect assessment aspect exist certain not enough.And be mostly individually in above method using discarded offal or cigarette
Grass blade bar does not carry out ratio optimization as raw material to the production of cigarettes such as offal, tobacco leaf fragment, offal leftover bits and pieces, have impact on cigarette
Produce the recycling efficiency of leftover bits and pieces.
The content of the invention
The present invention is exactly to overcome not enough present in above-mentioned technology of preparing, there is provided a kind of preparation method of tobacco extract, should
Method not only efficiently quick, energy-conserving and environment-protective, and obtained molecular distillation thing fragrance is true to nature, improves mouthfeel substantially, has in cigarette
There is preferable application effect.
The technical scheme that the present invention is provided：The method that a kind of utilization waste/hypo-tobacco leaf prepares tobacco extract, its feature exists
In comprising the following steps that：
(1) by offal between volume hired car：Tobacco leaf fragment：Offal is according to mass ratio 4~8:4~8:2~4 ratio is mixed
Close, prepare waste/hypo-tobacco leaf and extract raw material；
(2) second that the 6-15 times of concentration of weight is 30%-80% is added in extracting raw material to the waste/hypo-tobacco leaf for preparing
Alcoholic solution, is -0.04~-0.08MPa pressure is extracted, and under the conditions of 30-60 DEG C of temperature, extracts 2-6h, obtains waste/hypo-tobacco leaf thick
Extract；
(3) waste/hypo-tobacco leaf crude extract is concentrated under reduced pressure into substantially without ethanol flavor, then sequentially adds the pure of 4-8 times of its weight
The glucose of water purification, its weight 2%-6%, stirs, and obtains the waste/hypo-tobacco leaf crude extract aqueous solution；
(4) to sequentially added in waste/hypo-tobacco leaf solution of extract its weight 0.04%-0.4% cellulase and
The subtilopeptidase A of 0.02%-0.1%, 6- is persistently digested under the conditions of 30-45 DEG C, speed of agitator 90-150r/min
36h；
(5) digested in step (4) after terminating, to the ethanol solution of concentration 95% that 1-3 times of its weight is added in enzymolysis liquid,
Stirring 15-30min, stands 24-72h at ambient temperature；
(6) ethanol for obtaining step (5) stands liquid, using 100nm ceramic membrane filters, membrane filtration liquid is concentrated under reduced pressure into phase
It is 1.26~1.30 to density, prepares rough tobacco extract；
(7) to the propane diols that its weight 10%~30% is added in the rough tobacco extract prepared in step (6), mixing is equal
After even, molecular distillation pretreatment liquid is obtained；
(8) molecular distillation pretreatment liquid will be obtained in step (7) carries out molecular distillation separation, and separating technology parameter is：Very
Reciprocal of duty cycle≤2.0tor, scraper plate rotating speed to 20-70r/min, 30-55 DEG C of vapo(u)rizing temperature, condensation temperature -12~0 DEG C, distillation speed
0.8-4.0Kg/h, gained molecular distillation light component is waste/hypo-tobacco leaf tobacco extract of the present invention.
The present invention preferably technical scheme：The unit of activity of cellulase described in step (4)：10000U/g, withered grass
The unit of activity of Bacillus protease：1.8×105U/g。
Beneficial effects of the present invention：
(1) raw material in the present invention is mixed according to special ratio using offal, tobacco leaf fragment and offal, due to
Offal, tobacco leaf fragment and offal are workshop leftover bits and pieces, mix according to a certain percentage, both can simultaneously be followed three
Ring is utilized, and at utmost improves the recycling efficiency of leftover bits and pieces；
(2) present invention is dropped by cellulase and subtilopeptidase A to the cellulose and protein in tobacco
Solution, improves the product yield of tobacco extract, increases and improve the quality of tobacco extract product to varying degrees, improves volume
Tobacco quality；
(3) ethanol after present invention enzymolysis stands liquid and uses ceramic membrane filter, can remove filtering also middle high molecular weight protein
Matter, macromolecular cellulose and macromolecular polysaccharide, only have small molecule extremely by filtered fluid, and its volatility is more preferable；And adopt
With ceramic membrane filter, its filter efficiency is high, and effect is good, simple and convenient, economical and energy saving；
(4) medicinal extract crude product prepared by the present invention by molecular distillation become more meticulous isolating and purifying means again, enters traveling one
Step separation and purification treatment, causes the tobacco extract for preparing to exist necessarily not in terms of fragrance fidelity, cigarette effect assessment
Foot
It is an advantage of the invention that：Efficiently quick, energy-conserving and environment-protective, and obtained molecular distillation thing fragrance is true to nature, improves mouth
Sense is obvious, is used as essence spice for cigarette, can be obviously improved cigarette flavor matter, while also having soft fine and smooth flue gas, increases
Plus the sweet sense in oral cavity and improve the effect of the flue gas property sent out thoroughly.
Specific embodiment
With reference to embodiment, the invention will be further described.
Example 1, a kind of method that utilization waste/hypo-tobacco leaf prepares tobacco extract, comprises the following steps that：
(1) by offal between volume hired car：Tobacco leaf fragment：Offal=2：3：The ratio of 1 (mass ratio) is mixed, and is prepared useless time
Tobacco leaf extracts raw material；
(2) ethanol solution that raw material adds 7 times the 50% of weight is extracted to the waste/hypo-tobacco leaf for preparing, is extracting pressure
It is -0.04MPa, under the conditions of temperature 60 C, extracts 4h, obtains waste/hypo-tobacco leaf crude extract；
(3) waste/hypo-tobacco leaf crude extract is concentrated under reduced pressure into substantially without ethanol flavor, then sequentially adds the pure of 7 times of its weight
The glucose of water, its weight 4%, stirs, and obtains the waste/hypo-tobacco leaf crude extract aqueous solution；
(4) to the cellulase (unit of activity that its weight 0.1% is sequentially added in waste/hypo-tobacco leaf solution of extract：
10000U/g) subtilopeptidase A (the unit of activity with 0.06%：1.8×105U/g), in 32 DEG C, speed of agitator 120r/
12h is persistently digested under the conditions of min；
(5) after enzymolysis terminates, to 95% ethanol solution that 3 times of its weight is added in enzymolysis liquid, 15min is stirred, in room temperature
Under the conditions of stand 24h；
(6) ethanol for obtaining step (5) stands liquid, using 100nm ceramic membrane filters, membrane filtration liquid is concentrated under reduced pressure into phase
It is 1.29 to density, prepares rough tobacco extract；
(7) to the propane diols that its weight 25% is added in the rough tobacco extract in step (6), after being well mixed, obtain
Molecular distillation pretreatment liquid；
(8) the molecular distillation pretreatment liquid prepared in step (7) is carried out into molecular distillation separation, separating technology parameter is：
Vacuum 1.6tor, scraper plate rotating speed to 50r/min, 50 DEG C of vapo(u)rizing temperature, -5 DEG C of condensation temperature, distillation speed 2.2Kg/h, gained
Molecular distillation light component is waste/hypo-tobacco leaf tobacco extract of the invention.
Example 2, a kind of method that utilization waste/hypo-tobacco leaf prepares tobacco extract, comprises the following steps that：
(1) by offal between volume hired car：Tobacco leaf fragment：Offal=2：2：The ratio of 1 (mass ratio) is mixed, and is prepared useless time
Tobacco leaf extracts raw material；
(2) ethanol solution that raw material adds 15 times the 30% of weight is extracted to the waste/hypo-tobacco leaf prepared in step (1),
It is -0.06MPa pressure is extracted, under the conditions of temperature 45 C, extracts 5h, obtains waste/hypo-tobacco leaf crude extract；
(3) the waste/hypo-tobacco leaf crude extract in step (2) is concentrated under reduced pressure into substantially without ethanol flavor, then sequentially adds its heavy
Pure water, the glucose of its weight 2.5% of 4 times of amount, stir, and obtain the waste/hypo-tobacco leaf crude extract aqueous solution；
(4) to the cellulase that its weight 0.04% is sequentially added in the waste/hypo-tobacco leaf solution of extract in step (3)
(unit of activity：10000U/g) subtilopeptidase A (the unit of activity with 0.08%：1.8×105U/g), in 45 DEG C, stirring
24h is persistently digested under the conditions of rotating speed 100r/min；
(5) after enzymolysis terminates, to 95% ethanol solution that 1.5 times of its weight is added in enzymolysis liquid, 15min is stirred, in room
72h is stood under the conditions of temperature；
(6) ethanol for obtaining step (5) stands liquid, using 100nm ceramic membrane filters, membrane filtration liquid is concentrated under reduced pressure into phase
It is 1.265 to density, prepares rough tobacco extract；
(7) to the propane diols that its weight 10% is added in the rough tobacco extract prepared in step (6), after being well mixed,
Obtain molecular distillation pretreatment liquid；
(8) the molecular distillation pretreatment liquid obtained in step (7) is carried out into molecular distillation separation, separating technology parameter is：
Vacuum 0.8tor, scraper plate rotating speed to 30r/min, 55 DEG C of vapo(u)rizing temperature, -12 DEG C of condensation temperature, distillation speed 1.2Kg/h, institute
Obtain the waste/hypo-tobacco leaf tobacco extract that molecular distillation light component is in the present invention.
Example 3, a kind of method that utilization waste/hypo-tobacco leaf prepares tobacco extract, comprises the following steps that：
(1) by offal between volume hired car：Tobacco leaf fragment：Offal=6：4：The ratio of 3 (mass ratioes) is mixed, and is prepared useless time
Tobacco leaf extracts raw material；
(2) ethanol solution that raw material adds 10 times the 45% of weight is extracted to the waste/hypo-tobacco leaf prepared in step (1),
It is -0.04MPa pressure is extracted, under the conditions of 40 DEG C of temperature, extracts 2h, obtains waste/hypo-tobacco leaf crude extract；
(3) the waste/hypo-tobacco leaf crude extract obtained in step (2) is concentrated under reduced pressure into substantially without ethanol flavor, is then sequentially added
The pure water of 5 times of its weight, the glucose of its weight 5.5%, stir, and obtain the waste/hypo-tobacco leaf crude extract aqueous solution；
(4) to the fiber that its weight 0.15% is sequentially added in the waste/hypo-tobacco leaf solution of extract obtained in step (3)
Plain enzyme (unit of activity：10000U/g) subtilopeptidase A (the unit of activity with 0.02%：1.8×105U/g), 38 DEG C,
36h is persistently digested under the conditions of speed of agitator 90r/min；
(5) after enzymolysis terminates, to 95% ethanol solution that 3 times of its weight is added in enzymolysis liquid, 15min is stirred, in room temperature
Under the conditions of stand 72h；
(6) ethanol for obtaining step (5) stands liquid, using 100nm ceramic membrane filters, membrane filtration liquid is concentrated under reduced pressure into phase
It is 1.30 to density, prepares rough tobacco extract；
(7) to the propane diols that its weight 25% is added in the rough tobacco extract prepared in step (6), after being well mixed,
Obtain molecular distillation pretreatment liquid；
(8) molecular distillation pretreatment liquid is carried out into molecular distillation separation, separating technology parameter is：Vacuum 2.0tor, scrapes
Plate rotating speed is to 30r/min, 45 DEG C of vapo(u)rizing temperature, -10 DEG C of condensation temperature, distillation speed 1.2Kg/h, gained molecular distillation light component
As gained molecular distillation light component is the waste/hypo-tobacco leaf tobacco extract in the present invention.
Example 4：A kind of method that utilization waste/hypo-tobacco leaf prepares tobacco extract, comprises the following steps that：
(1) by offal between volume hired car：Tobacco leaf fragment：Offal=4：4：The ratio of 1 (mass ratio) is mixed, and is prepared useless time
Tobacco leaf extracts raw material；
(2) ethanol solution that raw material adds 15 times the 75% of weight is extracted to the waste/hypo-tobacco leaf for preparing, is pressed extracting
Power is -0.08MPa, under the conditions of 30 DEG C of temperature, extracts 4h, obtains waste/hypo-tobacco leaf crude extract；
(3) waste/hypo-tobacco leaf crude extract is concentrated under reduced pressure into substantially without ethanol flavor, then sequentially adds the pure of 4 times of its weight
The glucose of water, its weight 2%, stirs, and obtains the waste/hypo-tobacco leaf crude extract aqueous solution；
(4) to the cellulase (unit of activity that its weight 0.2% is sequentially added in waste/hypo-tobacco leaf solution of extract：
10000U/g) subtilopeptidase A (the unit of activity with 0.02%：1.8×105U/g), in 30 DEG C, speed of agitator 90r/
6h is persistently digested under the conditions of min；
(5) after enzymolysis terminates, to 95% ethanol solution that 1.5 times of its weight is added in enzymolysis liquid, 15min is stirred, in room
24h is stood under the conditions of temperature；
(6) precipitation solution for obtaining step (5), using 100nm ceramic membrane filters, membrane filtration liquid is concentrated under reduced pressure into relatively close
It is 1.278 to spend, and prepares rough tobacco extract；
(7) to the propane diols that its weight 15% is added in the rough tobacco extract prepared in step (6), after being well mixed,
Obtain molecular distillation pretreatment liquid；
(8) the molecular distillation pretreatment liquid obtained in step (7) is carried out into molecular distillation separation, separating technology parameter is：
Vacuum 1.0tor, scraper plate rotating speed to 60r/min, 30 DEG C of vapo(u)rizing temperature, condensation temperature DEG C, distillation speed 0.8Kg/h, institute's score
Son distillation light component is the waste/hypo-tobacco leaf tobacco extract in the present invention.
Example 5：A kind of method that utilization waste/hypo-tobacco leaf prepares tobacco extract, comprises the following steps that：
(1) by offal between volume hired car：Tobacco leaf fragment：Offal=1：2：The ratio of 1 (mass ratio) is mixed, and is prepared useless time
Tobacco leaf extracts raw material；
(2) ethanol solution that raw material adds 8 times the 30% of weight is extracted to the waste/hypo-tobacco leaf for preparing, is extracting pressure
It is -0.05MPa, under the conditions of temperature 45 C, extracts 3h, obtains waste/hypo-tobacco leaf crude extract；
(3) waste/hypo-tobacco leaf crude extract is concentrated under reduced pressure into substantially without ethanol flavor, then sequentially adds the pure of 8 times of its weight
The glucose of water, its weight 4.5%, stirs, and obtains the waste/hypo-tobacco leaf crude extract aqueous solution；
(4) to the cellulase (unit of activity that its weight 0.3% is sequentially added in waste/hypo-tobacco leaf solution of extract：
10000U/g) subtilopeptidase A (the unit of activity with 0.06%：1.8×105U/g), in 32 DEG C, speed of agitator 120r/
12h is persistently digested under the conditions of min；
(5) after enzymolysis terminates, to 95% ethanol solution that 2.5 times of its weight is added in enzymolysis liquid, 15min is stirred, in room
24h is stood under the conditions of temperature；
(6) ethanol for obtaining step (5) stands liquid, using 100nm ceramic membrane filters, membrane filtration liquid is concentrated under reduced pressure into phase
It is 1.283 to density, prepares rough tobacco extract；
(7) to the propane diols that its weight 25% is added in the rough tobacco extract prepared in step (6), after being well mixed,
Obtain molecular distillation pretreatment liquid；
(8) the molecular distillation pretreatment liquid obtained in step (7) is carried out into molecular distillation separation, separating technology parameter is：
Vacuum 1.8tor, scraper plate rotating speed to 60r/min, 40 DEG C of vapo(u)rizing temperature, -12 DEG C of condensation temperature, distillation speed 3.0Kg/h, institute
Obtain the waste/hypo-tobacco leaf tobacco extract that molecular distillation light component is in the present invention.
Step in embodiment 6, a kind of method that utilization waste/hypo-tobacco leaf prepares tobacco extract, specific steps and embodiment 1
(1) it is identical to step (6), obtain rough tobacco extract without the separated of step (7) and step (8).
The rough tobacco prepared in preparing waste/hypo-tobacco leaf tobacco extract, embodiment 6 in above-described embodiment 1 to embodiment 5
Medicinal extract, existing Nicotiana tabacum medicinal extract be respectively added to Yellow Crane Tower (soft indigo plant) experiment pipe tobacco in, by 5 professional and technical personnel
Smoke panel test, and sensory evaluation is carried out to sample pipe tobacco, its summarized results of smokeing panel test is shown in Table 1：
Table 1. is smoked panel test summarized results
Be can be seen that come crude extract prepared by the present invention has been able to reach existing common leaching by above-mentioned structure of smokeing panel test
The requirement of cream, the tobacco extract that the present invention finally gives is compared to existing Nicotiana tabacum medicinal extract and rough tobacco extract, its flue gas
Finer and smoother, aroma quality lifting is obvious, and mouthfeel is more fragrant and more sweet, comfortable.
Application is utilized respectively sheet frame, three kinds of filter types of centrifuge and ceramic membrane and product is processed, by filtering
Time-consuming, filtrate clarity, filtrate aesthetic quality are detected and are evaluated.Specific experiment the results are shown in Table 2.
The tobacco extract of table 2. difference filtering technique comparing result
* note:Above creation data is two batch averages.
Tobacco extract can be seen that by different filtering technique comparing results：1. from the point of view of filtration time, plate-frame filtering
＜ centrifugal filtration ＜ ceramic membrane filters, the plate-frame filtering time is most fast, but clarity is worst, place 12 hours after occur precipitation and by
It is cumulative many；2. centrifugal filtration is compared with ceramic membrane filter, and filtration time is very fast, but as standing time extension occurs a little sinking
Form sediment；3. after ceramic membrane filter, product clarification, it is bright, without precipitation after placement, and cigarette sensory effects are best.
Claims (2)
1. a kind of method that utilization waste/hypo-tobacco leaf prepares tobacco extract, it is characterised in that comprise the following steps that：
(1) by offal between volume hired car：Tobacco leaf fragment：Offal is according to mass ratio 4~8:4~8:2~4 ratio is mixed, system
Standby waste/hypo-tobacco leaf extracts raw material；
(2) the 6-15 times of concentration of weight is added for 30%-80% in extracting raw material to the waste/hypo-tobacco leaf prepared in step (1)
Ethanol solution, be -0.04~-0.08MPa pressure is extracted, under the conditions of 30-60 DEG C of temperature, extract 2-6h, obtain useless cigarette
Leaf crude extract；
(3) the waste/hypo-tobacco leaf crude extract obtained in step (2) is concentrated under reduced pressure into substantially without ethanol flavor, then sequentially adds its heavy
Pure water, the glucose of its weight 2%-6% of 4-8 times of amount, stir, and obtain the waste/hypo-tobacco leaf crude extract aqueous solution；
(4) to the fibre that its weight 0.04%-0.4% is sequentially added in the waste/hypo-tobacco leaf solution of extract obtained in step (3)
The subtilopeptidase A of the plain enzyme of dimension and 0.02%-0.1%, continues under the conditions of 30-45 DEG C, speed of agitator 90-150r/min
Enzymolysis 6-36h；
(5) digested in step (4) after terminating, to the ethanol solution of concentration 95% that 1-3 times of its weight is added in enzymolysis liquid, stirring
15-30min, stands 24-72h at ambient temperature；
(6) ethanol for obtaining step (5) stands liquid, using 100nm ceramic membrane filters, membrane filtration liquid is concentrated under reduced pressure into relatively close
It is 1.26~1.30 to spend, and prepares rough tobacco extract；
(7) to the propane diols that its weight 10%~30% is added in the rough tobacco extract prepared in step (6), it is well mixed
Afterwards, molecular distillation pretreatment liquid is obtained；
(8) molecular distillation pretreatment liquid will be obtained in step (7) carries out molecular distillation separation, and separating technology parameter is：Vacuum
≤ 2.0tor, scraper plate rotating speed to 20-70r/min, 30-55 DEG C of vapo(u)rizing temperature, condensation temperature -12~0 DEG C, distillation speed 0.8-
4.0Kg/h, gained molecular distillation light component is waste/hypo-tobacco leaf tobacco extract of the present invention.
2. the method that a kind of utilization waste/hypo-tobacco leaf according to claim 1 prepares tobacco extract, it is characterised in that：In step
(4) unit of activity of cellulase described in：10000U/g, the unit of activity of subtilopeptidase A：1.8×105U/g。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610375545.XA CN105852189B (en) | 2016-05-31 | 2016-05-31 | A kind of method that utilization waste/hypo-tobacco leaf prepares tobacco extract |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610375545.XA CN105852189B (en) | 2016-05-31 | 2016-05-31 | A kind of method that utilization waste/hypo-tobacco leaf prepares tobacco extract |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105852189A CN105852189A (en) | 2016-08-17 |
| CN105852189B true CN105852189B (en) | 2017-06-30 |
Family
ID=56643099
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610375545.XA Active CN105852189B (en) | 2016-05-31 | 2016-05-31 | A kind of method that utilization waste/hypo-tobacco leaf prepares tobacco extract |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105852189B (en) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106387981B (en) * | 2016-08-31 | 2017-07-21 | 湖北中烟工业有限责任公司 | A kind of integrated approach of General Promotion reconstituted tobacco quality |
| CN106307603B (en) * | 2016-09-20 | 2018-01-12 | 武汉黄鹤楼香精香料有限公司 | A kind of method that tobacco maillard reaction thing is prepared using natural plant extracts as raw material |
| CN106962969A (en) * | 2017-05-18 | 2017-07-21 | 山东瑞博斯烟草有限公司 | It is a kind of that the method that tobacco waste prepares reconstruction stem backfill feed liquid is handled by zymotechnic |
| CN108142984B (en) * | 2017-12-12 | 2021-02-19 | 湖北中烟工业有限责任公司 | Method for preparing tobacco leaf residue spice by fermenting tea-source golden florets |
| CN108354220A (en) * | 2018-03-07 | 2018-08-03 | 湖北中烟工业有限责任公司 | A kind of preparation method of Sun cured tobacco molecular distillation object |
| CN108384637B (en) * | 2018-03-13 | 2021-07-27 | 江西中烟工业有限责任公司 | Preparation method of bouquet type Maillard spice and application of bouquet type Maillard spice in cigarettes |
| CN109007957B (en) * | 2018-06-21 | 2021-10-29 | 重庆中烟工业有限责任公司 | Tobacco extract, preparation method thereof and tobacco product |
| CN110663993B (en) * | 2019-10-10 | 2022-04-22 | 福建中烟工业有限责任公司 | Tobacco extract and preparation method thereof |
| CN111876256A (en) * | 2020-07-16 | 2020-11-03 | 云南中烟工业有限责任公司 | Fresh and sweet flavor type tobacco essence and preparation method and application thereof |
| CN113040416A (en) * | 2021-02-02 | 2021-06-29 | 中国烟草总公司海南省公司 | Production method of cigar tobacco extract |
| CN115381132A (en) * | 2021-05-20 | 2022-11-25 | 湖南中烟工业有限责任公司 | Tobacco flavor prepared from tobacco and preparation method and application thereof |
| CN114788576B (en) * | 2022-04-07 | 2023-05-16 | 河南中烟工业有限责任公司 | Maillard reaction spice with outstanding roasted sweet fragrance and tobacco extract and preparation method and application thereof |
| CN115088860B (en) * | 2022-07-05 | 2023-06-20 | 贵州黄果树金叶科技有限公司 | Method for preparing tobacco absolute by enzymolysis of tobacco waste, tobacco absolute and application |
| CN115444162B (en) * | 2022-09-21 | 2024-04-19 | 安徽焦甜香生物科技有限公司 | Method for producing tobacco extract by using waste primary flue-cured tobacco leaves |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101204249B (en) * | 2007-12-11 | 2012-05-09 | 广东中烟工业有限责任公司 | Preparation method for tobacco extract |
| CN101416777B (en) * | 2008-11-28 | 2010-12-29 | 华芳烟用香料有限公司 | Preparation method of tobacco extractum and use thereof |
| CN101416778B (en) * | 2008-11-30 | 2011-05-18 | 华南理工大学 | Ferment for tobacco fermentation and use thereof |
| CN103251125B (en) * | 2013-05-06 | 2015-11-18 | 杭州利群环保纸业有限公司 | The manufacture method of the functional papermaking-method reconstituted tobaccos of a kind of high-quality |
| CN104351944B (en) * | 2014-10-22 | 2016-08-31 | 浙江中烟工业有限责任公司 | A kind of tobacco flavoring agent and methods and applications thereof |
| CN104757704B (en) * | 2014-12-31 | 2019-06-07 | 贵州中烟工业有限责任公司 | A kind of preparation method of tobacco extract and composition containing the tobacco extract |
| CN104839883B (en) * | 2015-05-31 | 2016-05-04 | 中国烟草总公司郑州烟草研究院 | A kind of tobacco juice for electronic smoke with pipe tobacco style and features |
| CN105146739B (en) * | 2015-07-28 | 2016-07-20 | 中国烟草总公司郑州烟草研究院 | The preparation method of a kind of mao of cigarette quintessence oil and application thereof |
-
2016
- 2016-05-31 CN CN201610375545.XA patent/CN105852189B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN105852189A (en) | 2016-08-17 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105852189B (en) | A kind of method that utilization waste/hypo-tobacco leaf prepares tobacco extract | |
| CN103349351B (en) | A kind of method of the papermaking-method reconstituted tobaccos based on membrane separation technique | |
| CN102613692B (en) | Method for recycling tobacco flavor from cigarette production waste | |
| CN102440432B (en) | Two-step microorganism fermentation method for preparing tobacco leachate | |
| CN100459889C (en) | Paper method leaching and thickening process for remaking tobacco leaf | |
| CN105212262A (en) | A kind of preparation method of tobacco flavor material | |
| CN103315377B (en) | A kind of preparation method of papermaking-method reconstituted tobaccos extract | |
| CN108142984A (en) | A kind of method for preparing tobacco leaf clout fragrance using the golden flower bacterium fermentation of tea source | |
| CN108669624A (en) | A kind of tobacco extract, preparation method and tobacco product | |
| CN103627523A (en) | Sun-cured red tobacco leaf extract, preparation method therefor and applications thereof in cigarettes | |
| CN106957880A (en) | A kind of immobilization beta glucuroide prepares method of the cigarette with safranine flower extract | |
| CN101607003B (en) | High-efficiency extraction method of effective ingredients in golden camellia tea and application thereof in cigarette preparation | |
| CN102276455B (en) | Method for extracting effective ingredients from dry cinnamomum burmannii fruits | |
| CN109619642B (en) | Tobacco extract for enhancing flavor of tobacco and preparation method thereof | |
| CN110839943A (en) | Roasted sweet and fragrant tobacco extract based on biological fermentation and preparation method thereof | |
| CN102090635A (en) | Method for preparing data flavone from red dates and data flavone | |
| CN106954886A (en) | A kind of preparation method of cigarette apple extract | |
| CN108148108B (en) | Method for extracting limonin from lemon peel residues by guanidine salt ionic liquid through two aqueous phases | |
| CN108402512B (en) | Electronic cigarette liquid and preparation method thereof | |
| CN102079773A (en) | Oleanolic acid and method for preparing oleanolic acid by utilizing red dates | |
| CN106883312A (en) | A kind of extracting method of silkworm excrement pectin | |
| CN106957725A (en) | A kind of preparation method of cigarette Fragaia ananassa Duchesne extract | |
| CN115444162B (en) | Method for producing tobacco extract by using waste primary flue-cured tobacco leaves | |
| CN113475746B (en) | Secondary extraction method of tobacco extract residues | |
| CN110839941B (en) | Pyrone-rich tobacco extract and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |