CN105852189A - Method for preparing tobacco extract by utilizing waste and inferior tobacco leaves - Google Patents
Method for preparing tobacco extract by utilizing waste and inferior tobacco leaves Download PDFInfo
- Publication number
- CN105852189A CN105852189A CN201610375545.XA CN201610375545A CN105852189A CN 105852189 A CN105852189 A CN 105852189A CN 201610375545 A CN201610375545 A CN 201610375545A CN 105852189 A CN105852189 A CN 105852189A
- Authority
- CN
- China
- Prior art keywords
- tobacco
- extract
- hypo
- waste
- tobacco leaf
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000000284 extract Substances 0.000 title claims abstract description 87
- 235000002637 Nicotiana tabacum Nutrition 0.000 title claims abstract description 86
- 241000208125 Nicotiana Species 0.000 title claims abstract description 73
- 239000002699 waste material Substances 0.000 title claims abstract description 64
- 238000000034 method Methods 0.000 title claims abstract description 30
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 66
- 238000000199 molecular distillation Methods 0.000 claims abstract description 33
- 230000000694 effects Effects 0.000 claims abstract description 16
- 238000003756 stirring Methods 0.000 claims abstract description 16
- 239000000919 ceramic Substances 0.000 claims abstract description 14
- 239000000203 mixture Substances 0.000 claims abstract description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 12
- 239000012634 fragment Substances 0.000 claims abstract description 11
- 108010059892 Cellulase Proteins 0.000 claims abstract description 10
- 108090000787 Subtilisin Proteins 0.000 claims abstract description 10
- 229940106157 cellulase Drugs 0.000 claims abstract description 10
- 238000000926 separation method Methods 0.000 claims abstract description 9
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract description 8
- 239000008103 glucose Substances 0.000 claims abstract description 8
- 239000007788 liquid Substances 0.000 claims description 29
- 239000000243 solution Substances 0.000 claims description 29
- 239000000287 crude extract Substances 0.000 claims description 20
- 239000002994 raw material Substances 0.000 claims description 18
- 239000012528 membrane Substances 0.000 claims description 13
- 238000002360 preparation method Methods 0.000 claims description 10
- 238000004821 distillation Methods 0.000 claims description 9
- 238000009833 condensation Methods 0.000 claims description 7
- 230000005494 condensation Effects 0.000 claims description 7
- 238000005374 membrane filtration Methods 0.000 claims description 7
- 108090000790 Enzymes Proteins 0.000 claims description 6
- 102000004190 Enzymes Human genes 0.000 claims description 6
- 239000007864 aqueous solution Substances 0.000 claims description 5
- 229940088598 enzyme Drugs 0.000 claims description 5
- 244000063299 Bacillus subtilis Species 0.000 claims 1
- 235000014469 Bacillus subtilis Nutrition 0.000 claims 1
- 235000019504 cigarettes Nutrition 0.000 abstract description 13
- 238000001914 filtration Methods 0.000 abstract description 11
- 238000002156 mixing Methods 0.000 abstract description 8
- 235000013599 spices Nutrition 0.000 abstract description 4
- 235000009508 confectionery Nutrition 0.000 abstract description 3
- 238000004519 manufacturing process Methods 0.000 abstract description 3
- 239000000779 smoke Substances 0.000 abstract description 3
- 210000000214 mouth Anatomy 0.000 abstract description 2
- 239000000843 powder Substances 0.000 abstract description 2
- 244000061176 Nicotiana tabacum Species 0.000 description 16
- DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical compound CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 description 15
- 239000003205 fragrance Substances 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- 239000000047 product Substances 0.000 description 4
- UGFAIRIUMAVXCW-UHFFFAOYSA-N Carbon monoxide Chemical compound [O+]#[C-] UGFAIRIUMAVXCW-UHFFFAOYSA-N 0.000 description 3
- 229920002678 cellulose Polymers 0.000 description 3
- 239000001913 cellulose Substances 0.000 description 3
- 238000000605 extraction Methods 0.000 description 3
- 239000000706 filtrate Substances 0.000 description 3
- 239000003546 flue gas Substances 0.000 description 3
- 238000001556 precipitation Methods 0.000 description 3
- 241000209134 Arundinaria Species 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 150000004676 glycans Chemical class 0.000 description 2
- 229920001282 polysaccharide Polymers 0.000 description 2
- 239000005017 polysaccharide Substances 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 238000010298 pulverizing process Methods 0.000 description 2
- 238000004064 recycling Methods 0.000 description 2
- 230000001953 sensory effect Effects 0.000 description 2
- SNICXCGAKADSCV-JTQLQIEISA-N (-)-Nicotine Chemical compound CN1CCC[C@H]1C1=CC=CN=C1 SNICXCGAKADSCV-JTQLQIEISA-N 0.000 description 1
- CWVRJTMFETXNAD-FWCWNIRPSA-N 3-O-Caffeoylquinic acid Natural products O[C@H]1[C@@H](O)C[C@@](O)(C(O)=O)C[C@H]1OC(=O)\C=C\C1=CC=C(O)C(O)=C1 CWVRJTMFETXNAD-FWCWNIRPSA-N 0.000 description 1
- 208000019901 Anxiety disease Diseases 0.000 description 1
- PZIRUHCJZBGLDY-UHFFFAOYSA-N Caffeoylquinic acid Natural products CC(CCC(=O)C(C)C1C(=O)CC2C3CC(O)C4CC(O)CCC4(C)C3CCC12C)C(=O)O PZIRUHCJZBGLDY-UHFFFAOYSA-N 0.000 description 1
- 244000025254 Cannabis sativa Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 244000283207 Indigofera tinctoria Species 0.000 description 1
- CWVRJTMFETXNAD-KLZCAUPSSA-N Neochlorogenin-saeure Natural products O[C@H]1C[C@@](O)(C[C@@H](OC(=O)C=Cc2ccc(O)c(O)c2)[C@@H]1O)C(=O)O CWVRJTMFETXNAD-KLZCAUPSSA-N 0.000 description 1
- 102000011759 adducin Human genes 0.000 description 1
- 108010076723 adducin Proteins 0.000 description 1
- 230000036506 anxiety Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- CWVRJTMFETXNAD-JUHZACGLSA-N chlorogenic acid Chemical compound O[C@@H]1[C@H](O)C[C@@](O)(C(O)=O)C[C@H]1OC(=O)\C=C\C1=CC=C(O)C(O)=C1 CWVRJTMFETXNAD-JUHZACGLSA-N 0.000 description 1
- 229940074393 chlorogenic acid Drugs 0.000 description 1
- FFQSDFBBSXGVKF-KHSQJDLVSA-N chlorogenic acid Natural products O[C@@H]1C[C@](O)(C[C@@H](CC(=O)C=Cc2ccc(O)c(O)c2)[C@@H]1O)C(=O)O FFQSDFBBSXGVKF-KHSQJDLVSA-N 0.000 description 1
- 235000001368 chlorogenic acid Nutrition 0.000 description 1
- BMRSEYFENKXDIS-KLZCAUPSSA-N cis-3-O-p-coumaroylquinic acid Natural products O[C@H]1C[C@@](O)(C[C@@H](OC(=O)C=Cc2ccc(O)cc2)[C@@H]1O)C(=O)O BMRSEYFENKXDIS-KLZCAUPSSA-N 0.000 description 1
- 238000005352 clarification Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 239000012043 crude product Substances 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 238000002481 ethanol extraction Methods 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- -1 iginates Species 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 229960002715 nicotine Drugs 0.000 description 1
- SNICXCGAKADSCV-UHFFFAOYSA-N nicotine Natural products CN1CCCC1C1=CC=CN=C1 SNICXCGAKADSCV-UHFFFAOYSA-N 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000010992 reflux Methods 0.000 description 1
- 238000010025 steaming Methods 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B3/00—Preparing tobacco in the factory
- A24B3/14—Forming reconstituted tobacco products, e.g. wrapper materials, sheets, imitation leaves, rods, cakes; Forms of such products
Landscapes
- Manufacture Of Tobacco Products (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Vaporization, Distillation, Condensation, Sublimation, And Cold Traps (AREA)
Abstract
The invention provides a method for preparing tobacco extract by utilizing waste and inferior tobacco leaves. The tobacco extract is prepared through the following steps: mixing tobacco powder, tobacco leaf fragments and tobacco stems in a cigarette manufacturing department and adding the mixture into an ethanol solution for extracting; decompressing and concentrating; then adding pure water and glucose in sequence; after uniformly stirring, adding cellulase and subtilisin in sequence to carry out enzymolysis; then adding the mixture subject to enzymolysis into the ethanol solution and standing; filtering with a ceramic film; and decompressing and concentrating and then carrying out molecular distillation and separation. According to the method, efficient, rapid, energy-saving and environment-friendly effects are achieved; prepared molecular distillate has a real aroma and the taste is remarkably improved; the product is used as essence and spice for cigarettes so that the aroma of the cigarettes can be obviously improved; and meanwhile, the product also has soft, fine and smooth smoke and has the effects of improving the sweet feeling of the oral cavity and improving the smoke volatility.
Description
Technical field
The present invention relates to the preparation method of a kind of tobacco extract, specifically one and utilize waste/hypo-tobacco leaf
The method preparing tobacco extract.
Technical background
Nicotiana tabacum L., for Solanaceae Nicotiana plant, originates in South America, introduces a fine variety relatively early in China, and yield is big.
At present, China's tobacco planting area and yield of tobacco all occupy first place in the world, and relate to the relevant of tobacco business
Income plays an important role in the national economic development of China.Nicotiana tabacum L. plantation, gather,
During transport, storage and Nicotiana tabacum L. processing etc., there is the generation of a large amount of waste/hypo-tobacco leaf, account for Nicotiana tabacum L. and always produce
The 25%-30% of amount, particularity of chemical composition contained by it, if dealing with improperly, not only waste resource,
The most easily pollute environment.Knowable to current document report, experts and scholars are with waste/hypo-tobacco leaf as raw material,
Use different technical methods, extract the compositions such as Salanesol therein, nicotine, chlorogenic acid, Yi Jili
Prepare fertilizer, feedstuff and biological pesticide etc. with waste/hypo-tobacco leaf, but above method technique is complex, former
Material utilization rate is the highest, easily causes environmental pollution.
Currently also have and many patents disclose some by waste/hypo-tobacco leaf for the method preparing tobacco extract,
Such as Patent No.: disclosed in CN201210412335.5, a kind of tobacco waste prepares the side of tobacco extract
Method, the method is directly by the steaming and decocting that adds water after the pulverizing of discarded offal, redistillation after then processing with compound enzyme
Concentrate；Patent No.: a kind of tobacco extract disclosed in CN201210059368.6 and extracting method thereof and
In application, the tobacco extract in this patent is to being added by Nicotiana tabacum L. cane bast powder after ethanol extraction again
Filter and add after neutral protein enzyme extraction, re-use ethanol and carry out distillation as extractant and obtain；Patent
Number it is: the preparation method of a kind of tobacco extract disclosed in CN200710032351.0 that the method is will be useless
Abandon offal add water and enzyme carry out enzymolysis, be subsequently adding alcohol reflux, use thin after filtering and concentrating
Film evaporimeter anxiety is evaporated, and obtains tobacco extract by Maillard reaction after reconcentration；The patent No.:
A kind of method utilizing enzyme to prepare tobacco extract disclosed in CN2005100295668, the method is to adopt
With offal, discarded tobacco leaf and Nicotiana tabacum L., to be raw material add water after pulverizing carries out enzymolysis, to enzymolysis
Filtrate carries out concentration and obtains.Although said method can also prepare tobacco extract, but prepares
Tobacco extract in there are a lot of macro-molecular protein, macromole fiber and macromolecular polysaccharide, these things
Matter should not be volatilized, and is deposited in extractum, affects the volatility of extractum spice, reduces the soft of spice fragrance
With property and fine and smooth degree, cause the tobacco extract prepared in fragrance fidelity, Medicated cigarette effect assessment
Aspect exists certain not enough.And above method is mostly individually use discarded offal or Nicotiana tabacum L. cane
As raw material, the production of cigarettes leftover bits and pieces such as offal, tobacco leaf fragment, cabo are not carried out ratio optimization,
Have impact on the recycling efficiency of production of cigarettes leftover bits and pieces.
Summary of the invention
The present invention overcomes deficiency present in above-mentioned technology of preparing exactly, it is provided that the system of a kind of tobacco extract
Preparation Method, not only the quickest, the energy-conserving and environment-protective of this method, and also the molecular distillation thing fragrance prepared is true to nature,
Improve mouthfeel obvious, have in Medicated cigarette and preferably apply effect.
The technical scheme that the present invention provides: a kind of described method utilizing waste/hypo-tobacco leaf to prepare tobacco extract,
It is characterized in that specifically comprising the following steps that
(1) by offal between volume hired car: tobacco leaf fragment: cabo is according to the ratio of mass ratio 4～8:4～8:2～4
Mix, prepare waste/hypo-tobacco leaf and extract raw material；
(2) extracting the concentration of 6-15 times of weight of addition in raw material to the waste/hypo-tobacco leaf prepared is
The ethanol solution of 30%-80%, is-0.04～-0.08MPa extracting pressure, temperature 30-60 DEG C condition
Under, extract 2-6h, obtain waste/hypo-tobacco leaf crude extract；
(3) it is evaporated to waste/hypo-tobacco leaf crude extract, substantially without ethanol taste, then be sequentially added into it heavy
Measure pure water, the glucose of its weight 2%-6% of 4-8 times, stir, obtain waste/hypo-tobacco leaf and slightly carry
Thing aqueous solution；
(4) in waste/hypo-tobacco leaf solution of extract, it is sequentially added into the cellulose of its weight 0.04%-0.4%
Enzyme and the subtilisin of 0.02%-0.1%, at 30-45 DEG C, speed of agitator 90-150r/min
Under the conditions of continue enzymolysis 6-36h；
(5), after enzymolysis terminates in step (4), in enzymolysis solution, the concentration of its weight 1-3 times is added
95% ethanol solution, stirs 15-30min, stands 24-72h at ambient temperature；
(6) ethanol step (5) obtained stands liquid, uses 100nm ceramic membrane filter, by membrane filtration
It is 1.26～1.30 that liquid is evaporated to relative density, prepares rough tobacco extract；
(7) the rough tobacco extract of preparation adds in the step (6) the third the two of its weight 10%～30%
Alcohol, after mix homogeneously, obtains molecular distillation pretreatment liquid；
(8) carry out molecular distillation separation by step (7) obtains molecular distillation pretreatment liquid, separate work
Skill parameter is: vacuum≤2.0tor, scraper plate rotating speed to 20-70r/min, vapo(u)rizing temperature 30-55 DEG C,
Condensation temperature-12～0 DEG C, distillation speed 0.8-4.0Kg/h, the light component of gained molecular distillation is this
Bright described waste/hypo-tobacco leaf tobacco extract.
The present invention preferably technical scheme: the unit of activity of cellulase described in step (4):
10000U/g, the unit of activity of subtilisin: 1.8 × 105U/g。
Beneficial effects of the present invention:
(1) raw material in the present invention is to use offal, tobacco leaf fragment and cabo to mix according to special ratio
Conjunction forms, owing to offal, tobacco leaf fragment and cabo are workshop leftover bits and pieces, according to a certain percentage
Mixing, both can be circulated three utilization simultaneously, at utmost improve the recycling of leftover bits and pieces
Efficiency；
(2) present invention passes through cellulase and subtilisin to the cellulose in Nicotiana tabacum L. and albumen
Matter is degraded, and improves the product yield of tobacco extract, increases to varying degrees and improve cigarette
The quality of grass extract product, improves cigarette quality；
(3) ethanol after enzymolysis of the present invention stands liquid and uses ceramic membrane filter, can remove in filtration also
Macro-molecular protein, macromole cellulose and macromolecular polysaccharide, only have little point in filtered fluid
Extremely, its volatility is more preferable for son；And using ceramic membrane filter, its filter efficiency is high, effective, letter
Folk prescription just, economical and energy saving；
(4) the extractum crude product that prepared by the present invention carries out, by molecular distillation, the isolated and purified hands that becomes more meticulous again
Section, carries out further separation and purification treatment, cause the tobacco extract for preparing fragrance fidelity,
Medicated cigarette effect assessment aspect exists certain not enough
The invention have the advantage that efficiently quickly, energy-conserving and environment-protective, and the molecular distillation thing fragrance prepared
True to nature, improve mouthfeel obvious, use as essence spice for cigarette, it is possible to be obviously improved cigarette flavor matter,
The most also there is soft fine and smooth flue gas, increase the sweet sense in oral cavity and improve the effect of the flue gas property sent out thoroughly.
Detailed description of the invention
Below in conjunction with embodiment, the invention will be further described.
Example 1, a kind of method utilizing waste/hypo-tobacco leaf to prepare tobacco extract, specifically comprise the following steps that
(1) by offal between volume hired car: tobacco leaf fragment: the ratio of cabo=2:3:1 (mass ratio) is carried out
Mixing, prepares waste/hypo-tobacco leaf and extracts raw material；
(2) extract, to the waste/hypo-tobacco leaf prepared, the ethanol solution that raw material adds the 50% of 7 times of weight,
It is-0.04MPa extracting pressure, under the conditions of temperature 60 C, extracts 4h, obtain waste/hypo-tobacco leaf crude extract；
(3) it is evaporated to waste/hypo-tobacco leaf crude extract, substantially without ethanol taste, then be sequentially added into its weight
The pure water of 7 times, the glucose of its weight 4%, stir, obtain waste/hypo-tobacco leaf crude extract water-soluble
Liquid；
(4) in waste/hypo-tobacco leaf solution of extract, it is sequentially added into cellulase (the vigor of its weight 0.1%
Unit: 10000U/g) and the subtilisin (unit of activity: 1.8 × 10 of 0.06%5U/g),
32 DEG C, continue enzymolysis 12h under the conditions of speed of agitator 120r/min；
(5) after enzymolysis terminates, in enzymolysis solution, add 95% ethanol solution of its weight 3 times, stir 15min,
Stand 24h at ambient temperature；
(6) ethanol step (5) obtained stands liquid, uses 100nm ceramic membrane filter, by membrane filtration
It is 1.29 that liquid is evaporated to relative density, prepares rough tobacco extract；
(7) the rough tobacco extract in step (6) adds the propylene glycol of its weight 25%, mixing
After Jun Yun, obtain molecular distillation pretreatment liquid；
(8) the molecular distillation pretreatment liquid of preparation in step (7) is carried out molecular distillation separation, separate
Technological parameter is: vacuum 1.6tor, scraper plate rotating speed to 50r/min, vapo(u)rizing temperature 50 DEG C, condensation
Temperature-5 DEG C, distillation speed 2.2Kg/h, the light component of gained molecular distillation is the waste/hypo-tobacco leaf of the present invention
Tobacco extract.
Example 2, a kind of method utilizing waste/hypo-tobacco leaf to prepare tobacco extract, specifically comprise the following steps that
(1) by offal between volume hired car: tobacco leaf fragment: the ratio of cabo=2:2:1 (mass ratio) is carried out
Mixing, prepares waste/hypo-tobacco leaf and extracts raw material；
(2) waste/hypo-tobacco leaf prepared in step (1) extracts raw material and adds the 30% of 15 times of weight
Ethanol solution, extract pressure be-0.06MPa, under the conditions of temperature 45 C, extraction 5h, given up
Hypo-tobacco leaf crude extract；
(3) the waste/hypo-tobacco leaf crude extract in step (2) is evaporated to substantially without ethanol taste, then
It is sequentially added into the pure water of its weight 4 times, the glucose of its weight 2.5%, stirs, given up
Hypo-tobacco leaf crude extract aqueous solution；
(4) the waste/hypo-tobacco leaf solution of extract in step (3) is sequentially added into its weight 0.04%
Cellulase (unit of activity: 10000U/g) and 0.08% subtilisin (unit of activity:
1.8×105U/g), 45 DEG C, continue enzymolysis 24h under the conditions of speed of agitator 100r/min；
(5) after enzymolysis terminates, in enzymolysis solution, 95% ethanol solution of its weight 1.5 times is added, stirring
15min, stands 72h at ambient temperature；
(6) ethanol step (5) obtained stands liquid, uses 100nm ceramic membrane filter, by membrane filtration
It is 1.265 that liquid is evaporated to relative density, prepares rough tobacco extract；
(7) the rough tobacco extract of preparation adds in step (6) propylene glycol of its weight 10%,
After mix homogeneously, obtain molecular distillation pretreatment liquid；
(8) the molecular distillation pretreatment liquid obtained in step (7) is carried out molecular distillation separation, separate
Technological parameter is: vacuum 0.8tor, scraper plate rotating speed to 30r/min, vapo(u)rizing temperature 55 DEG C, condensation
Temperature-12 DEG C, distillation speed 1.2Kg/h, it is useless secondary that the light component of gained molecular distillation is in the present invention
Nicotiana tabacum L. tobacco extract.
Example 3, a kind of method utilizing waste/hypo-tobacco leaf to prepare tobacco extract, specifically comprise the following steps that
(1) by offal between volume hired car: tobacco leaf fragment: the ratio of cabo=6:4:3 (mass ratio) is carried out
Mixing, prepares waste/hypo-tobacco leaf and extracts raw material；
(2) waste/hypo-tobacco leaf prepared in step (1) extracts raw material and adds the 45% of 10 times of weight
Ethanol solution, extract pressure be-0.04MPa, under the conditions of temperature 40 DEG C, extraction 2h, given up
Hypo-tobacco leaf crude extract；
(3) the waste/hypo-tobacco leaf crude extract obtained in step (2) is evaporated to substantially without ethanol taste,
Then it is sequentially added into the pure water of its weight 5 times, the glucose of its weight 5.5%, stirs,
To waste/hypo-tobacco leaf crude extract aqueous solution；
(4) the waste/hypo-tobacco leaf solution of extract obtained in step (3) is sequentially added into its weight
The cellulase (unit of activity: 10000U/g) of 0.15% and the subtilisin of 0.02% (are lived
Unit of force: 1.8 × 105U/g), 38 DEG C, continue enzymolysis 36h under the conditions of speed of agitator 90r/min；
(5) after enzymolysis terminates, in enzymolysis solution, 95% ethanol solution of its weight 3 times is added, stirring
15min, stands 72h at ambient temperature；
(6) ethanol step (5) obtained stands liquid, uses 100nm ceramic membrane filter, by membrane filtration
It is 1.30 that liquid is evaporated to relative density, prepares rough tobacco extract；
(7) the rough tobacco extract of preparation adds in step (6) propylene glycol of its weight 25%,
After mix homogeneously, obtain molecular distillation pretreatment liquid；
(8) molecular distillation pretreatment liquid carrying out molecular distillation separation, separating technology parameter is: vacuum
Degree 2.0tor, scraper plate rotating speed to 30r/min, vapo(u)rizing temperature 45 DEG C, condensation temperature-10 DEG C, distillation speed
Degree 1.2Kg/h, the light component of gained molecular distillation is the light component of gained molecular distillation and is in the present invention
Waste/hypo-tobacco leaf tobacco extract.
Example 4: a kind of method utilizing waste/hypo-tobacco leaf to prepare tobacco extract, specifically comprises the following steps that
(1) by offal between volume hired car: tobacco leaf fragment: the ratio of cabo=4:4:1 (mass ratio) is entered
Row mixing, prepares waste/hypo-tobacco leaf and extracts raw material；
(2) extract, to the waste/hypo-tobacco leaf prepared, the ethanol solution that raw material adds the 75% of 15 times of weight,
It is-0.08MPa extracting pressure, under the conditions of temperature 30 DEG C, extracts 4h, obtain waste/hypo-tobacco leaf crude extract；
(3) it is evaporated to waste/hypo-tobacco leaf crude extract, substantially without ethanol taste, then be sequentially added into it heavy
Measure pure water, the glucose of its weight 2% of 4 times, stir, obtain waste/hypo-tobacco leaf crude extract water
Solution；
(4) cellulase being sequentially added into its weight 0.2% in waste/hypo-tobacco leaf solution of extract (is lived
Unit of force: 10000U/g) and the subtilisin (unit of activity: 1.8 × 10 of 0.02%5U/g),
30 DEG C, continue enzymolysis 6h under the conditions of speed of agitator 90r/min；
(5) after enzymolysis terminates, in enzymolysis solution, 95% ethanol solution of its weight 1.5 times is added, stirring
15min, stands 24h at ambient temperature；
(6) alcohol deposit fluid step (5) obtained, uses 100nm ceramic membrane filter, is subtracted by membrane filtration liquid
It is 1.278 that pressure is concentrated into relative density, prepares rough tobacco extract；
(7) the rough tobacco extract of preparation adds in step (6) propylene glycol of its weight 15%,
After mix homogeneously, obtain molecular distillation pretreatment liquid；
(8) the molecular distillation pretreatment liquid obtained in step (7) is carried out molecular distillation separation, separate
Technological parameter is: vacuum 1.0tor, scraper plate rotating speed to 60r/min, vapo(u)rizing temperature 30 DEG C, condensation
Temperature DEG C, distillation speed 0.8Kg/h, the waste/hypo-tobacco leaf that the light component of gained molecular distillation is in the present invention
Tobacco extract.
Example 5: a kind of method utilizing waste/hypo-tobacco leaf to prepare tobacco extract, specifically comprises the following steps that
(1) by offal between volume hired car: tobacco leaf fragment: the ratio of cabo=1:2:1 (mass ratio) is entered
Row mixing, prepares waste/hypo-tobacco leaf and extracts raw material；
(2) extract, to the waste/hypo-tobacco leaf prepared, the ethanol solution that raw material adds the 30% of 8 times of weight,
It is-0.05MPa extracting pressure, under the conditions of temperature 45 C, extracts 3h, obtain waste/hypo-tobacco leaf crude extract；
(3) it is evaporated to waste/hypo-tobacco leaf crude extract, substantially without ethanol taste, then be sequentially added into it heavy
Measure pure water, the glucose of its weight 4.5% of 8 times, stir, obtain waste/hypo-tobacco leaf crude extract
Aqueous solution；
(4) cellulase being sequentially added into its weight 0.3% in waste/hypo-tobacco leaf solution of extract (is lived
Unit of force: 10000U/g) and the subtilisin (unit of activity: 1.8 × 10 of 0.06%5U/g),
32 DEG C, continue enzymolysis 12h under the conditions of speed of agitator 120r/min；
(5) after enzymolysis terminates, in enzymolysis solution, 95% ethanol solution of its weight 2.5 times is added, stirring
15min, stands 24h at ambient temperature；
(6) ethanol step (5) obtained stands liquid, uses 100nm ceramic membrane filter, by membrane filtration
It is 1.283 that liquid is evaporated to relative density, prepares rough tobacco extract；
(7) the rough tobacco extract of preparation adds in step (6) propylene glycol of its weight 25%,
After mix homogeneously, obtain molecular distillation pretreatment liquid；
(8) the molecular distillation pretreatment liquid obtained in step (7) is carried out molecular distillation separation, separate
Technological parameter is: vacuum 1.8tor, scraper plate rotating speed to 60r/min, vapo(u)rizing temperature 40 DEG C, condensation
Temperature-12 DEG C, distillation speed 3.0Kg/h, it is useless secondary that the light component of gained molecular distillation is in the present invention
Nicotiana tabacum L. tobacco extract.
Embodiment 6, a kind of method utilizing waste/hypo-tobacco leaf to prepare tobacco extract, concrete steps and embodiment
Step (1) in 1 is identical to step (6), divides without the distillation of step (7) and step (8)
From obtaining rough tobacco extract.
Make preparing by above-described embodiment 1 to embodiment 5 in waste/hypo-tobacco leaf tobacco extract, embodiment 6
Standby rough tobacco extract, the Yellow Crane Tower (soft indigo plant) that is respectively added to of existing Nicotiana tabacum extractum try
Test in tobacco shred, smoke panel test through 5 professional and technical personnel, and sample tobacco shred is carried out sensory evaluation, its
Summarized results of smokeing panel test is shown in Table 1:
Table 1. is smoked panel test summarized results
Be can be seen that by above-mentioned structure of smokeing panel test, rough extractum prepared by the present invention has been able to reach existing
Having the requirement of ordinary extract, the tobacco extract that the present invention finally gives compares existing Nicotiana tabacum extractum
With rough tobacco extract, its flue gas is finer and smoother, aroma quality promotes substantially, and mouthfeel is more fragrant and more sweet, easypro
Suitable.
Application is utilized respectively sheet frame, centrifuge and three kinds of filter types of ceramic membrane and processes product, logical
Cross filtering time-consuming, filtrate clarity, filtrate aesthetic quality detects and evaluates.Specific experiment is tied
Fruit is shown in Table 2.
Table 2. tobacco extract difference filtering technique comparing result
* note: above creation data is two batch average.
Tobacco extract by different filtering technique comparing results it can be seen that 1. from the point of view of filtration time,
Plate-and-frame filtration ＜ centrifugal filtration ＜ ceramic membrane filter, the plate-and-frame filtration time is the fastest, but clarity is worst,
Precipitation occurs after placing 12 hours and gradually increases；2. centrifugal filtration is compared with ceramic membrane filter, filters
Time is very fast, but there will be a little precipitation along with extending standing time；3., after ceramic membrane filter, produce
Product clarification, bright, without precipitation after placement, and Medicated cigarette sensory effects is best.
Claims (2)
1. one kind utilizes the method that waste/hypo-tobacco leaf prepares tobacco extract, it is characterised in that specifically comprise the following steps that
(1) by offal between volume hired car: tobacco leaf fragment: cabo is according to the ratio of mass ratio 4～8:4～8:2～4
Mix, prepare waste/hypo-tobacco leaf and extract raw material；
(2) waste/hypo-tobacco leaf prepared in step (1) extracts and adds 6-15 times of weight in raw material
The ethanol solution that concentration is 30%-80%, extract pressure be-0.04～-0.08MPa, temperature
Under the conditions of 30-60 DEG C, extract 2-6h, obtain waste/hypo-tobacco leaf crude extract；
(3) the waste/hypo-tobacco leaf crude extract obtained in step (2) is evaporated to substantially without ethanol taste,
Then it is sequentially added into the glucose of the pure water of its weight 4-8 times, its weight 2%-6%, stirs,
Obtain waste/hypo-tobacco leaf crude extract aqueous solution；
(4) the waste/hypo-tobacco leaf solution of extract obtained in step (3) is sequentially added into its weight
The cellulase of 0.04%-0.4% and the subtilisin of 0.02%-0.1%, 30-45 DEG C, stirring
Enzymolysis 6-36h is continued under the conditions of rotating speed 90-150r/min；
(5), after enzymolysis terminates in step (4), in enzymolysis solution, the concentration of its weight 1-3 times is added
95% ethanol solution, stirs 15-30min, stands 24-72h at ambient temperature；
(6) ethanol step (5) obtained stands liquid, uses 100nm ceramic membrane filter, by membrane filtration
It is 1.26～1.30 that liquid is evaporated to relative density, prepares rough tobacco extract；
(7) the rough tobacco extract of preparation adds in the step (6) the third the two of its weight 10%～30%
Alcohol, after mix homogeneously, obtains molecular distillation pretreatment liquid；
(8) carry out molecular distillation separation by step (7) obtains molecular distillation pretreatment liquid, separate work
Skill parameter is: vacuum≤2.0tor, scraper plate rotating speed to 20-70r/min, vapo(u)rizing temperature 30-55 DEG C,
Condensation temperature-12～0 DEG C, distillation speed 0.8-4.0Kg/h, the light component of gained molecular distillation is this
Bright described waste/hypo-tobacco leaf tobacco extract.
A kind of method utilizing waste/hypo-tobacco leaf to prepare tobacco extract the most according to claim 1, it is special
Levy and be: the unit of activity of cellulase: 10000U/g, bacillus subtilis egg described in step (4)
The unit of activity of white enzyme: 1.8 × 105U/g。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610375545.XA CN105852189B (en) | 2016-05-31 | 2016-05-31 | A kind of method that utilization waste/hypo-tobacco leaf prepares tobacco extract |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610375545.XA CN105852189B (en) | 2016-05-31 | 2016-05-31 | A kind of method that utilization waste/hypo-tobacco leaf prepares tobacco extract |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105852189A true CN105852189A (en) | 2016-08-17 |
| CN105852189B CN105852189B (en) | 2017-06-30 |
Family
ID=56643099
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610375545.XA Active CN105852189B (en) | 2016-05-31 | 2016-05-31 | A kind of method that utilization waste/hypo-tobacco leaf prepares tobacco extract |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105852189B (en) |
Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106307603A (en) * | 2016-09-20 | 2017-01-11 | 武汉黄鹤楼香精香料有限公司 | Method for preparing Maillard reactant for cigarettes by taking natural plant extract as raw material |
| CN106387981A (en) * | 2016-08-31 | 2017-02-15 | 湖北中烟工业有限责任公司 | Comprehensive method for all-around improvement of quality of reconstituted tobacco |
| CN106962969A (en) * | 2017-05-18 | 2017-07-21 | 山东瑞博斯烟草有限公司 | It is a kind of that the method that tobacco waste prepares reconstruction stem backfill feed liquid is handled by zymotechnic |
| CN108142984A (en) * | 2017-12-12 | 2018-06-12 | 湖北中烟工业有限责任公司 | A kind of method for preparing tobacco leaf clout fragrance using the golden flower bacterium fermentation of tea source |
| CN108354220A (en) * | 2018-03-07 | 2018-08-03 | 湖北中烟工业有限责任公司 | A kind of preparation method of Sun cured tobacco molecular distillation object |
| CN108384637A (en) * | 2018-03-13 | 2018-08-10 | 江西中烟工业有限责任公司 | A kind of preparation method of aroma type maillard flavour and its application in cigarette |
| CN109007957A (en) * | 2018-06-21 | 2018-12-18 | 重庆中烟工业有限责任公司 | A kind of tobacco extract, preparation method and tobacco product |
| CN110663993A (en) * | 2019-10-10 | 2020-01-10 | 福建中烟工业有限责任公司 | Tobacco extract and preparation method thereof |
| CN111876256A (en) * | 2020-07-16 | 2020-11-03 | 云南中烟工业有限责任公司 | Fresh and sweet flavor type tobacco essence and preparation method and application thereof |
| CN113040416A (en) * | 2021-02-02 | 2021-06-29 | 中国烟草总公司海南省公司 | Production method of cigar tobacco extract |
| CN114788576A (en) * | 2022-04-07 | 2022-07-26 | 河南中烟工业有限责任公司 | Yuyan extract Maillard reaction spice with outstanding roasted sweet aroma and preparation method and application thereof |
| CN115088860A (en) * | 2022-07-05 | 2022-09-23 | 贵州黄果树金叶科技有限公司 | Method for preparing tobacco absolute oil by treating tobacco waste through enzymolysis, tobacco absolute oil and application |
| CN115381132A (en) * | 2021-05-20 | 2022-11-25 | 湖南中烟工业有限责任公司 | Tobacco flavor prepared from tobacco and preparation method and application thereof |
| CN115444162A (en) * | 2022-09-21 | 2022-12-09 | 安徽焦甜香生物科技有限公司 | Method for producing tobacco extract by utilizing waste flue-cured tobacco leaves |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101204249A (en) * | 2007-12-11 | 2008-06-25 | 广东中烟工业公司 | Preparation method for tobacco extracting |
| CN101416777A (en) * | 2008-11-28 | 2009-04-29 | 华芳烟用香料有限公司 | Preparation method of tobacco extractum and use thereof |
| CN101416778A (en) * | 2008-11-30 | 2009-04-29 | 华南理工大学 | Ferment for tobacco fermentation and use thereof |
| CN103251125A (en) * | 2013-05-06 | 2013-08-21 | 杭州利群环保纸业有限公司 | Method of manufacturing reconstituted tobacco through high-quality functional paper-making process |
| CN104351944A (en) * | 2014-10-22 | 2015-02-18 | 浙江中烟工业有限责任公司 | Tobacco perfuming agent prepared through molecular distillation treatment of extractum obtained through Maillard reaction from inferior tobacco leaves, preparation method and application of tobacco perfuming agent |
| CN104757704A (en) * | 2014-12-31 | 2015-07-08 | 贵州中烟工业有限责任公司 | Preparation method for tobacco extract and composition with the tobacco extract |
| CN104839883A (en) * | 2015-05-31 | 2015-08-19 | 中国烟草总公司郑州烟草研究院 | Electronic cigarette liquid with characteristics of tobaccos for tobacco pipes |
| CN105146739A (en) * | 2015-07-28 | 2015-12-16 | 中国烟草总公司郑州烟草研究院 | Preparation method and application of Jin Tang tobacco essential oil |
-
2016
- 2016-05-31 CN CN201610375545.XA patent/CN105852189B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101204249A (en) * | 2007-12-11 | 2008-06-25 | 广东中烟工业公司 | Preparation method for tobacco extracting |
| CN101416777A (en) * | 2008-11-28 | 2009-04-29 | 华芳烟用香料有限公司 | Preparation method of tobacco extractum and use thereof |
| CN101416778A (en) * | 2008-11-30 | 2009-04-29 | 华南理工大学 | Ferment for tobacco fermentation and use thereof |
| CN103251125A (en) * | 2013-05-06 | 2013-08-21 | 杭州利群环保纸业有限公司 | Method of manufacturing reconstituted tobacco through high-quality functional paper-making process |
| CN104351944A (en) * | 2014-10-22 | 2015-02-18 | 浙江中烟工业有限责任公司 | Tobacco perfuming agent prepared through molecular distillation treatment of extractum obtained through Maillard reaction from inferior tobacco leaves, preparation method and application of tobacco perfuming agent |
| CN104757704A (en) * | 2014-12-31 | 2015-07-08 | 贵州中烟工业有限责任公司 | Preparation method for tobacco extract and composition with the tobacco extract |
| CN104839883A (en) * | 2015-05-31 | 2015-08-19 | 中国烟草总公司郑州烟草研究院 | Electronic cigarette liquid with characteristics of tobaccos for tobacco pipes |
| CN105146739A (en) * | 2015-07-28 | 2015-12-16 | 中国烟草总公司郑州烟草研究院 | Preparation method and application of Jin Tang tobacco essential oil |
Cited By (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106387981A (en) * | 2016-08-31 | 2017-02-15 | 湖北中烟工业有限责任公司 | Comprehensive method for all-around improvement of quality of reconstituted tobacco |
| CN106307603B (en) * | 2016-09-20 | 2018-01-12 | 武汉黄鹤楼香精香料有限公司 | A kind of method that tobacco maillard reaction thing is prepared using natural plant extracts as raw material |
| CN106307603A (en) * | 2016-09-20 | 2017-01-11 | 武汉黄鹤楼香精香料有限公司 | Method for preparing Maillard reactant for cigarettes by taking natural plant extract as raw material |
| CN106962969A (en) * | 2017-05-18 | 2017-07-21 | 山东瑞博斯烟草有限公司 | It is a kind of that the method that tobacco waste prepares reconstruction stem backfill feed liquid is handled by zymotechnic |
| CN108142984A (en) * | 2017-12-12 | 2018-06-12 | 湖北中烟工业有限责任公司 | A kind of method for preparing tobacco leaf clout fragrance using the golden flower bacterium fermentation of tea source |
| CN108354220A (en) * | 2018-03-07 | 2018-08-03 | 湖北中烟工业有限责任公司 | A kind of preparation method of Sun cured tobacco molecular distillation object |
| CN108384637B (en) * | 2018-03-13 | 2021-07-27 | 江西中烟工业有限责任公司 | Preparation method of bouquet type Maillard spice and application of bouquet type Maillard spice in cigarettes |
| CN108384637A (en) * | 2018-03-13 | 2018-08-10 | 江西中烟工业有限责任公司 | A kind of preparation method of aroma type maillard flavour and its application in cigarette |
| CN109007957B (en) * | 2018-06-21 | 2021-10-29 | 重庆中烟工业有限责任公司 | Tobacco extract, preparation method thereof and tobacco product |
| CN109007957A (en) * | 2018-06-21 | 2018-12-18 | 重庆中烟工业有限责任公司 | A kind of tobacco extract, preparation method and tobacco product |
| CN110663993A (en) * | 2019-10-10 | 2020-01-10 | 福建中烟工业有限责任公司 | Tobacco extract and preparation method thereof |
| CN110663993B (en) * | 2019-10-10 | 2022-04-22 | 福建中烟工业有限责任公司 | Tobacco extract and preparation method thereof |
| CN111876256A (en) * | 2020-07-16 | 2020-11-03 | 云南中烟工业有限责任公司 | Fresh and sweet flavor type tobacco essence and preparation method and application thereof |
| CN113040416A (en) * | 2021-02-02 | 2021-06-29 | 中国烟草总公司海南省公司 | Production method of cigar tobacco extract |
| CN115381132A (en) * | 2021-05-20 | 2022-11-25 | 湖南中烟工业有限责任公司 | Tobacco flavor prepared from tobacco and preparation method and application thereof |
| CN114788576A (en) * | 2022-04-07 | 2022-07-26 | 河南中烟工业有限责任公司 | Yuyan extract Maillard reaction spice with outstanding roasted sweet aroma and preparation method and application thereof |
| CN115088860A (en) * | 2022-07-05 | 2022-09-23 | 贵州黄果树金叶科技有限公司 | Method for preparing tobacco absolute oil by treating tobacco waste through enzymolysis, tobacco absolute oil and application |
| CN115444162A (en) * | 2022-09-21 | 2022-12-09 | 安徽焦甜香生物科技有限公司 | Method for producing tobacco extract by utilizing waste flue-cured tobacco leaves |
| CN115444162B (en) * | 2022-09-21 | 2024-04-19 | 安徽焦甜香生物科技有限公司 | Method for producing tobacco extract by using waste primary flue-cured tobacco leaves |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105852189B (en) | 2017-06-30 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105852189A (en) | Method for preparing tobacco extract by utilizing waste and inferior tobacco leaves | |
| CN103349351B (en) | A kind of method of the papermaking-method reconstituted tobaccos based on membrane separation technique | |
| CN105212262A (en) | A kind of preparation method of tobacco flavor material | |
| CN102613692B (en) | Method for recycling tobacco flavor from cigarette production waste | |
| CN100459889C (en) | Paper method leaching and thickening process for remaking tobacco leaf | |
| CN106036982B (en) | A kind of preparation method and applications of cigarette bay leaf extract | |
| CN105686069B (en) | A kind of tobacco extract and preparation method thereof and its purposes | |
| CN108794651A (en) | The method that pectin is extracted in passion fruit pericarp | |
| CN103315377B (en) | A kind of preparation method of papermaking-method reconstituted tobaccos extract | |
| CN112646661A (en) | Preparation method and application of smoked plum tobacco flavor | |
| CN113633019B (en) | Cigar-flavored tobacco extract and preparation method thereof | |
| CN111808681A (en) | Preparation method of lycium ruthenicum murr spice for cigarettes and application of lycium ruthenicum murr spice in cigarettes | |
| CN108142984B (en) | Method for preparing tobacco leaf residue spice by fermenting tea-source golden florets | |
| CN102321503B (en) | Preparation method of fructus momordicae condensate and application thereof | |
| CN105146741B (en) | A kind of natural herbal enzymolysis liquid and preparation method thereof and the application in cigarette | |
| CN101322582B (en) | Pu'er raw tea alcohol-extracted extract and preparation and use thereof | |
| CN110742302B (en) | Cigarette capable of reducing free radical content in smoke and preparation method thereof | |
| CN108497547A (en) | A kind of preparation method and applications of the cigarette liquor tailing fragrance of tool Dark sun-cured fragrance | |
| CN104017646A (en) | Preparation method and application of malliard reaction product of waste and inferior tobacco leaf concentrate | |
| CN108477662B (en) | Manufacturing process of reconstituted tobacco with burley tobacco characteristics | |
| CN110663993B (en) | Tobacco extract and preparation method thereof | |
| CN109619642B (en) | Tobacco extract for enhancing flavor of tobacco and preparation method thereof | |
| CN114176247A (en) | Method for preparing tobacco paste by using waste tobacco extract | |
| CN106954886A (en) | A kind of preparation method of cigarette apple extract | |
| CN102079773A (en) | Oleanolic acid and method for preparing oleanolic acid by utilizing red dates |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |