CN106387981A - Comprehensive method for all-around improvement of quality of reconstituted tobacco - Google Patents
Comprehensive method for all-around improvement of quality of reconstituted tobacco Download PDFInfo
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- CN106387981A CN106387981A CN201610781804.9A CN201610781804A CN106387981A CN 106387981 A CN106387981 A CN 106387981A CN 201610781804 A CN201610781804 A CN 201610781804A CN 106387981 A CN106387981 A CN 106387981A
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- Prior art keywords
- reconstituted tobacco
- quality
- tobacco
- mass ratio
- nicotine
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Links
- 235000002637 Nicotiana tabacum Nutrition 0.000 title claims abstract description 135
- 241000208125 Nicotiana Species 0.000 title claims abstract description 121
- 238000000034 method Methods 0.000 title claims abstract description 14
- SNICXCGAKADSCV-JTQLQIEISA-N (-)-Nicotine Chemical compound CN1CCC[C@H]1C1=CC=CN=C1 SNICXCGAKADSCV-JTQLQIEISA-N 0.000 claims abstract description 34
- 229960002715 nicotine Drugs 0.000 claims abstract description 34
- SNICXCGAKADSCV-UHFFFAOYSA-N nicotine Natural products CN1CCCC1C1=CC=CN=C1 SNICXCGAKADSCV-UHFFFAOYSA-N 0.000 claims abstract description 34
- 102000004190 Enzymes Human genes 0.000 claims abstract description 32
- 108090000790 Enzymes Proteins 0.000 claims abstract description 32
- 239000011347 resin Substances 0.000 claims abstract description 30
- 229920005989 resin Polymers 0.000 claims abstract description 30
- 241000894006 Bacteria Species 0.000 claims abstract description 28
- 239000002253 acid Substances 0.000 claims abstract description 27
- 239000000843 powder Substances 0.000 claims abstract description 22
- 239000000758 substrate Substances 0.000 claims abstract description 19
- 101001065065 Aspergillus awamori Feruloyl esterase A Proteins 0.000 claims abstract description 15
- 241000194108 Bacillus licheniformis Species 0.000 claims abstract description 14
- 108010054320 Lignin peroxidase Proteins 0.000 claims abstract description 14
- 239000002689 soil Substances 0.000 claims abstract description 14
- 238000000855 fermentation Methods 0.000 claims abstract description 12
- 230000004151 fermentation Effects 0.000 claims abstract description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 10
- 241000228245 Aspergillus niger Species 0.000 claims abstract description 9
- 238000001035 drying Methods 0.000 claims abstract description 8
- 230000008569 process Effects 0.000 claims abstract description 8
- 239000007787 solid Substances 0.000 claims abstract description 6
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 46
- 235000013305 food Nutrition 0.000 claims description 25
- 239000000284 extract Substances 0.000 claims description 24
- 239000004310 lactic acid Substances 0.000 claims description 23
- 235000014655 lactic acid Nutrition 0.000 claims description 23
- 238000000605 extraction Methods 0.000 claims description 17
- 238000013459 approach Methods 0.000 claims description 16
- 235000019504 cigarettes Nutrition 0.000 claims description 15
- 244000061176 Nicotiana tabacum Species 0.000 claims description 14
- 230000000694 effects Effects 0.000 claims description 13
- 239000007788 liquid Substances 0.000 claims description 13
- 238000010521 absorption reaction Methods 0.000 claims description 12
- 238000002360 preparation method Methods 0.000 claims description 12
- 239000000835 fiber Substances 0.000 claims description 11
- 238000001179 sorption measurement Methods 0.000 claims description 10
- 238000009288 screen filtration Methods 0.000 claims description 8
- 239000002131 composite material Substances 0.000 claims description 7
- 239000007864 aqueous solution Substances 0.000 claims description 5
- 239000012141 concentrate Substances 0.000 claims description 5
- 239000004744 fabric Substances 0.000 claims description 5
- 239000002245 particle Substances 0.000 claims description 5
- 230000000391 smoking effect Effects 0.000 claims description 3
- 201000002909 Aspergillosis Diseases 0.000 claims description 2
- 208000036641 Aspergillus infections Diseases 0.000 claims description 2
- 239000000779 smoke Substances 0.000 abstract description 6
- 150000001875 compounds Chemical class 0.000 abstract description 3
- 238000001914 filtration Methods 0.000 abstract description 2
- 241000186660 Lactobacillus Species 0.000 abstract 1
- 230000007794 irritation Effects 0.000 abstract 1
- 229940039696 lactobacillus Drugs 0.000 abstract 1
- 238000005096 rolling process Methods 0.000 abstract 1
- 229940088598 enzyme Drugs 0.000 description 27
- 229920005610 lignin Polymers 0.000 description 20
- 150000002500 ions Chemical class 0.000 description 18
- 210000002421 cell wall Anatomy 0.000 description 11
- 239000001913 cellulose Substances 0.000 description 8
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- 229920001282 polysaccharide Polymers 0.000 description 8
- 239000005017 polysaccharide Substances 0.000 description 8
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 7
- 150000004676 glycans Chemical class 0.000 description 7
- 238000004519 manufacturing process Methods 0.000 description 7
- 238000012360 testing method Methods 0.000 description 7
- KSEBMYQBYZTDHS-HWKANZROSA-M (E)-Ferulic acid Natural products COC1=CC(\C=C\C([O-])=O)=CC=C1O KSEBMYQBYZTDHS-HWKANZROSA-M 0.000 description 6
- 235000001785 ferulic acid Nutrition 0.000 description 6
- 229940114124 ferulic acid Drugs 0.000 description 6
- KSEBMYQBYZTDHS-UHFFFAOYSA-N ferulic acid Natural products COC1=CC(C=CC(O)=O)=CC=C1O KSEBMYQBYZTDHS-UHFFFAOYSA-N 0.000 description 6
- 239000008103 glucose Substances 0.000 description 6
- -1 saccharide compound Chemical class 0.000 description 6
- QURCVMIEKCOAJU-UHFFFAOYSA-N trans-isoferulic acid Natural products COC1=CC=C(C=CC(O)=O)C=C1O QURCVMIEKCOAJU-UHFFFAOYSA-N 0.000 description 6
- UGFAIRIUMAVXCW-UHFFFAOYSA-N Carbon monoxide Chemical compound [O+]#[C-] UGFAIRIUMAVXCW-UHFFFAOYSA-N 0.000 description 5
- 239000003546 flue gas Substances 0.000 description 5
- 239000003205 fragrance Substances 0.000 description 5
- 239000000047 product Substances 0.000 description 5
- 239000000126 substance Substances 0.000 description 5
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 4
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 4
- 239000012634 fragment Substances 0.000 description 4
- 229920001277 pectin Polymers 0.000 description 4
- 239000001814 pectin Substances 0.000 description 4
- 235000010987 pectin Nutrition 0.000 description 4
- 235000018102 proteins Nutrition 0.000 description 4
- 102000004169 proteins and genes Human genes 0.000 description 4
- 108090000623 proteins and genes Proteins 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 241000193830 Bacillus <bacterium> Species 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
- 229920002488 Hemicellulose Polymers 0.000 description 3
- 108091005804 Peptidases Proteins 0.000 description 3
- 239000004365 Protease Substances 0.000 description 3
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 3
- 229920002472 Starch Polymers 0.000 description 3
- 229910052799 carbon Inorganic materials 0.000 description 3
- 230000008859 change Effects 0.000 description 3
- KSEBMYQBYZTDHS-HWKANZROSA-N ferulic acid Chemical compound COC1=CC(\C=C\C(O)=O)=CC=C1O KSEBMYQBYZTDHS-HWKANZROSA-N 0.000 description 3
- 230000007062 hydrolysis Effects 0.000 description 3
- 238000006460 hydrolysis reaction Methods 0.000 description 3
- 230000002779 inactivation Effects 0.000 description 3
- 229920002521 macromolecule Polymers 0.000 description 3
- 230000000813 microbial effect Effects 0.000 description 3
- 230000009467 reduction Effects 0.000 description 3
- 239000008107 starch Substances 0.000 description 3
- 235000019698 starch Nutrition 0.000 description 3
- NGSWKAQJJWESNS-UHFFFAOYSA-N 4-coumaric acid Chemical compound OC(=O)C=CC1=CC=C(O)C=C1 NGSWKAQJJWESNS-UHFFFAOYSA-N 0.000 description 2
- IKHGUXGNUITLKF-UHFFFAOYSA-N Acetaldehyde Chemical compound CC=O IKHGUXGNUITLKF-UHFFFAOYSA-N 0.000 description 2
- 239000004382 Amylase Substances 0.000 description 2
- 102000013142 Amylases Human genes 0.000 description 2
- 108010065511 Amylases Proteins 0.000 description 2
- 108010059892 Cellulase Proteins 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- 235000001014 amino acid Nutrition 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- 235000019418 amylase Nutrition 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 229940106157 cellulase Drugs 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 239000000796 flavoring agent Substances 0.000 description 2
- 235000019634 flavors Nutrition 0.000 description 2
- 238000009432 framing Methods 0.000 description 2
- 239000007789 gas Substances 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 229920001542 oligosaccharide Polymers 0.000 description 2
- 230000000149 penetrating effect Effects 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 230000001953 sensory effect Effects 0.000 description 2
- AUJXJFHANFIVKH-GQCTYLIASA-N trans-methylferulate Chemical compound COC(=O)\C=C\C1=CC=C(O)C(OC)=C1 AUJXJFHANFIVKH-GQCTYLIASA-N 0.000 description 2
- HGINCPLSRVDWNT-UHFFFAOYSA-N Acrolein Chemical compound C=CC=O HGINCPLSRVDWNT-UHFFFAOYSA-N 0.000 description 1
- GUBGYTABKSRVRQ-XLOQQCSPSA-N Alpha-Lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 description 1
- 206010006784 Burning sensation Diseases 0.000 description 1
- 206010011224 Cough Diseases 0.000 description 1
- 206010013786 Dry skin Diseases 0.000 description 1
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 1
- 235000008331 Pinus X rigitaeda Nutrition 0.000 description 1
- 235000011613 Pinus brutia Nutrition 0.000 description 1
- 241000018646 Pinus brutia Species 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 1
- FRYDSOYOHWGSMD-UHFFFAOYSA-N [C].O Chemical compound [C].O FRYDSOYOHWGSMD-UHFFFAOYSA-N 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 239000000853 adhesive Substances 0.000 description 1
- 230000001070 adhesive effect Effects 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 244000144987 brood Species 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 239000011285 coke tar Substances 0.000 description 1
- 238000005336 cracking Methods 0.000 description 1
- 238000004132 cross linking Methods 0.000 description 1
- MLUCVPSAIODCQM-NSCUHMNNSA-N crotonaldehyde Chemical compound C\C=C\C=O MLUCVPSAIODCQM-NSCUHMNNSA-N 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 102000038379 digestive enzymes Human genes 0.000 description 1
- 108091007734 digestive enzymes Proteins 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 235000008216 herbs Nutrition 0.000 description 1
- 230000003301 hydrolyzing effect Effects 0.000 description 1
- 230000000937 inactivator Effects 0.000 description 1
- 238000002329 infrared spectrum Methods 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 239000008101 lactose Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 125000000956 methoxy group Chemical group [H]C([H])([H])O* 0.000 description 1
- AUJXJFHANFIVKH-UHFFFAOYSA-N methyl cis-ferulate Natural products COC(=O)C=CC1=CC=C(O)C(OC)=C1 AUJXJFHANFIVKH-UHFFFAOYSA-N 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 150000002482 oligosaccharides Chemical class 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 150000002989 phenols Chemical class 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 210000000697 sensory organ Anatomy 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000001117 sulphuric acid Substances 0.000 description 1
- 235000011149 sulphuric acid Nutrition 0.000 description 1
- 210000004243 sweat Anatomy 0.000 description 1
- 238000004227 thermal cracking Methods 0.000 description 1
- 235000019505 tobacco product Nutrition 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B3/00—Preparing tobacco in the factory
- A24B3/14—Forming reconstituted tobacco products, e.g. wrapper materials, sheets, imitation leaves, rods, cakes; Forms of such products
Landscapes
- Manufacture Of Tobacco Products (AREA)
Abstract
The invention provides a comprehensive method for all-around improvement of quality of reconstituted tobacco. The comprehensive method comprises the following steps: (1) compound fermentation: in a reconstituted tobacco extracting process, fermenting a reconstituted tobacco water solution with a compound of aspergillus niger, feruloyl esterase, soil lignin peroxidase, bacillus licheniformis powder and freeze-dried lactobacillus powder; (2) adsorbing nicotine in an extracting solution by virtue of strong acid in-exchange macroporous resin for 30-60min, and conducting filtering to remove the nicotine by virtue of a sieve so as to obtain an extracting solution; (3) concentrating the reconstituted tobacco in a vacuum environment at 75-85 DEG C until a solid content is 40%, so as to obtain a concentrated solution; (4) drying residues, which are obtained by extracting the reconstituted tobacco, by virtue of a dryer at 100-120 DEG C, and deactivating enzyme and bacteria so as to obtain tobacco substrates; and (5) rolling the tobacco substrates. With the application of the comprehensive method provided by the invention, the produced reconstituted tobacco is low in irritation, mellow in taste and smell, elegant in aroma, light in green miscellaneous gas and smooth in smoke, and the all-around improvement of the quality of the reconstituted tobacco is achieved.
Description
Technical field
The present invention relates to tobacco industry field, specifically a kind of integrated approach of General Promotion reconstituted tobacco quality.
Background technology
Cell wall substance mainly has cellulose, hemicellulose, pectin etc..Cell wall substance and content of starch are high, and Nicotiana tabacum L. is thick
Rough, flue gas stimulates greatly, and discord is suitable.Water-soluble sugar content height makes the jealous alcohol of Nicotiana tabacum L. and fragrance grace, but saccharide compound content
Height, releasing content of coke tar is big.In cigarette product, excessive cellulose can give flue gas spine and burning sensation and produce to choke and cough.Fine
Dimension element can be generated glucose, oligosaccharide etc. by enzyme directionally hydrolyzing, can effectively reduce the zest of Medicated cigarette, mitigate miscellaneous QI and change
Kind suction taste, but sugar excessively also can make tarry matters in smoke amount increase.Lignin is one of main macromolecular substances of tobacco cell wall, cigarette
The thermal cracking of vegetation quality has a negative impact to cigarette quality, and content of lignin is higher, and quality of tobacco is poorer, has strong
Penetrating odor, blue foreign smell weight, inhale peppery, the puckery mouth of acrid in the mouth, during its cracking, produce small molecule aldehyde and phenol type substances, such as propylene
Aldehyde, phenol, 1,2- Benzodiazepiness etc..Simple phenols is often relevant with puckery mouth, flavour of a drug, shows medicinal herbs gas, chemistry breath and coarse
Suction taste effect, acrylic aldehyde and acetaldehyde then can bring stimulation, pungent jealous to Medicated cigarette.Lignin is also paper industry discharge simultaneously
The main cause that black liquor COD and colourity are formed, its structure be by the p- hydroxycinnamic acid of methoxy substitution be polymerized heterogeneous
Polycrystalline three-dimensional polymer, intermolecular mostly stable ehter bond, C-C key, are the microorganism difficult degradation aromatics generally acknowledged at present
One of.
Nicotine is topmost biological active substanceies in Nicotiana tabacum L., take in appropriate nicotine can refresh oneself excitement, brace up,
Eliminate tense situation etc., the good Nicotiana tabacum L. of inherent quality and tobacco product contain appropriate nicotine, by smoker with suitable physiology
The fragrance that intensity is become reconciled with jealous.If nicotine content is too low, strength is little, inhales taste flat；If nicotine content height, strength is big, thorn
Sharp property strengthens, and produces acid.
Reconstituted tobacco mainly adds adhesive and other additives etc. to form by offal, fragment, cabo or discarded tobacco leaf.Cigarette
End, fragment, cabo are the by-products of tobacco industry, in the past make Disposal more.China is Nicotiana tabacum L. big country, has few hundred thousand tonnes of every year
Offal, fragment, cabo resource go out of use, and have both caused environmental pollution, are also the waste to natural resourcess simultaneously.Research shows, cigarette
End, fragment, the composition species containing in cabo and tobacco leaf ingredient are basically identical, but have that miscellaneous QI weight, zest be big and mouthfeel
Discomfort etc. inhales taste defect.
Feruloyl esterase can cut off the crosslinking in cell wall between polysaccharide one polysaccharide, polysaccharide one lignin, is conducive to cell wall
The release of the degraded of polysaccharide and lignin in material, using the raw material of feruloyl esterase process vegetal, can be by ferulic acid
Separate out from the structure of plant cell wall, thus destroy cell wall framing structure so that lignin, hemicellulose with
And the connection between cellulose partly broken, structure becomes more loose than before processing.Ferulic acid is present in cured tobacco leaf.Brood cell
Bacillus can produce multiple digestive enzyme, has stronger protease, amylase activity, also have pectase, glucanase, fibre simultaneously
The plain enzyme isoreactivity of dimension, can destroy the cell wall of plant cell, promote the release of active substance.Bacillus licheniformis enzyme system includes
Protease, pectase, cellulase etc..Lactic acid bacteria (lactic acid bacteria, LAB) is that a class can utilize fermentable carbon
Hydrate produces the common name of the antibacterial of a large amount of lactic acid, can produce lactic acid etc. organic from the sweat of glucose or Lactose
Acid.
Using aspergillus niger feruloyl esterase, soil lignin peroxidase (S-Lip), bacillus licheniformis, lactic acid bacteria
Composite fermentation reconstituted tobacco extract, then using the nicotine in strong-acid type macroporous resin adsorption extract, and removed with screen filtration
Go to adsorb the macroporous resin of nicotine, General Promotion reconstituted tobacco quality.Wherein, the enzyme system of enzyme and Bacillus licheniformis is come
Macromolecular substances such as lignin, polysaccharide, the protein etc. in reconstituted tobacco extract and substrate, lactic acid bacteria fermentation Portugal are decomposed in conversion
Grape sugar changes into lactic acid, and lactic acid combines nicotine, and macroporous resin adsorption nicotine is simultaneously filtered to remove, and reduces smoke pH, makes production
Reconstituted tobacco reduce zest, jealous alcohol and, fragrance is graceful, reduce blue foreign smell, flue gas and suitable, improves reconstituted tobacco sense organ product
Matter, have not been reported.
Content of the invention
The present invention is directed to the deficiencies in the prior art, provides a kind of integrated approach of General Promotion reconstituted tobacco quality, adopts
Aspergillus niger feruloyl esterase, soil lignin peroxidase (S-Lip), bacillus licheniformis, lactic acid bacteria composite fermentation reproduce
Nicotiana tabacum L. extract, then using the nicotine in strong-acid type macroporous resin adsorption extract, and remove absorption nicotine with screen filtration
Macroporous resin, the quality of General Promotion reconstituted tobacco.Wherein enzyme and microbial enzyme system decompose reconstituted tobacco extraction to convert
Macromolecular substances such as lignin, polysaccharide, protein, cellulose, pectin etc. in liquid and substrate is taken to be that glucose, aminoacid etc. are little
Molecular substance, then lactic acid bacteria fermentation glucose changes into the organic acid such as lactic acid, in conjunction with nicotine, then macroporous resin adsorption nicotine is simultaneously
Be filtered to remove, reduce smoke pH, make the reconstituted tobacco of production reduce zest, jealous alcohol and, fragrance is graceful, it is blue or green miscellaneous to reduce
Gas, flue gas and suitable, raising reconstituted tobacco organoleptic quality.
The present invention provides a kind of integrated approach of General Promotion reconstituted tobacco quality, comprises the following steps：
(1) composite fermentation：In the extraction process of reconstituted tobacco, by the amount with the 0.14～0.4% of reconstituted tobacco mass ratio,
Aspergillus niger feruloyl esterase, soil lignin peroxidase (S-Lip), bacillus licheniformis powder, freeze-dried lactic acid bacteria powder are pressed
Certain proportion is added to mix homogeneously in reconstituted tobacco aqueous solution, adjusts pH to 4.5～6.0, under the conditions of 35～45 DEG C, fermentation
1～12 hour, obtain reconstituted tobacco extract；
(2) strong acid ion macropore exchanger resin absorption：In the reconstituted tobacco extract of above-mentioned steps (1), add and account for again
Make the strong acid ion macropore exchanger resin that quality of tobacco is than 0.1～0.3%, under the conditions of pH4.5～6.0,55～65 DEG C, absorption
Nicotine 30～60min in extract, removes the strong acid ion macroporous resin of absorption nicotine, obtains reconstituted tobacco through screen filtration
Extract；
(3) concentrate：It is concentrated in vacuo to solid content 40% by 75～85 DEG C of above-mentioned steps (2) extract, obtain improving and inhale
The reproduced tobacco concentrated liquid of flavor quality, in the process, more than 70 DEG C, the enzyme of the present invention and bacterium inactivate temperature；
(4) substrate preparation：Residue after extraction for the reconstituted tobacco, by being implemented at 100～120 DEG C of dryings with drying machine
Reason, inactivator and bacterium, manufacture the baccy fiber of the water content being adjusted to certain, baccy fiber are configured to sheet and make cigarette
Careless substrate.
(5) roll：The concentrated solution of step (3) is sprayed to and on the Nicotiana tabacum L. substrate of step (4), rolls into reconstituted tobacco.
Further, the aspergillus niger feruloyl esterase described in step (1) is food stage, and enzyme activity 100-1000u/g accounts for again
The mass ratio making Nicotiana tabacum L. is 0.05～0.15%；Soil lignin peroxidase (S-Lip), food stage, enzyme activity 100～
3000u/g, accounts for the 0.05～0.15% of reconstituted tobacco mass ratio；Bacillus licheniformis powder, food stage, 100,000,000,000～500,000,000,000/g
Vigor, accounting for reconstituted tobacco mass ratio is 0.02～0.05%；Freeze-dried lactic acid bacteria powder, food stage, 100,000,000,000～200,000,000,000/g vigor,
Accounting for reconstituted tobacco mass ratio is 0.02～0.05%.
Further, the strong acid ion macropore exchanger resin described in step (2) is food stage, particle size range 0.3～
1.2mm, preferably HZ016 etc.；Screen cloth is 60 mesh～120 mesh, diameter 0.125mm～0.250mm.
Further, the certain water content described in step (4) is mass ratio 5～15%.
Further, the certain water content described in step (4) is mass ratio 11～13%.
Beneficial effects of the present invention：
The present invention adopt enzyme and microbial enzyme system hydrolysis, coupled ion macroporous resin adsorption complex art,
By in ferulic acid ester enzyme hydrolysiss offal, the Ferulic acid methylester of cabo cell wall, oligosaccharide ferulic acid ester and polysaccharide ferulic acid ester
Ester bond, ferulic acid separate out, the degraded beneficial to polysaccharide in cell wall substance and the release of lignin destroy cell wall
Framing structure, so that the connection between lignin, hemicellulose and cellulose is partly broken, structure become than before processing dredge
Pine, the protease in the compound enzyme system of bacillus licheniformis, pectase, cellulase, amylase etc., can degrade and reproduce simultaneously
Protein in Nicotiana tabacum L. and its extract, pectin, starch and cellulose, generate free amino acid, glucose etc., lactic acid bacteria then turns
Change glucose and generate lactic acid, bound fraction nicotine；Reconstituted tobacco and extraction and soil lignin peroxidase (S-Lip) can be degraded
Take lignin in liquid so as to content reduces, reduce penetrating odor, reduce blue foreign smell and coarse suction taste, pungent jealous；Strong acid
Ion macroporous resin energy bound fraction nicotine, removes through screen filtration, then can reduce nicotine content, reduces acid, retains
Appropriate nicotine, by the fragrance become reconciled with suitable physiological strength to smoker with jealous.
Present invention process mild condition easy to operate it is achieved that biological enzymolysis, microorganism conversion and chemisorbed complex art,
General Promotion reconstituted tobacco quality, has the prospect of industrial applications.
Specific embodiment
Below by specific embodiment, the technical scheme in the present invention is clearly and completely described.It should be appreciated that
This partly described specific embodiment can only be used to explain the present invention, is not intended to limit the present invention.
Embodiment 1
The present invention provides a kind of reconstituted tobacco of integrated approach General Promotion quality, and its preparation comprises the following steps：
(1) composite fermentation：In the extraction process of reconstituted tobacco, by the amount with the 0.14% of reconstituted tobacco mass ratio, will be black
Aspergillosis feruloyl esterase, soil lignin peroxidase (S-Lip), bacillus licheniformis powder, freeze-dried lactic acid bacteria powder are pressed certain
Ratio is added to mix homogeneously in reconstituted tobacco aqueous solution, adjusts pH to 4.5, under the conditions of 35 DEG C, ferments 3 hours；
Described aspergillus niger feruloyl esterase is food stage, enzyme activity 1000u/g, and the mass ratio accounting for reconstituted tobacco is
0.05%；Soil lignin peroxidase (S-Lip), food stage, enzyme activity 3000u/g, account for reconstituted tobacco mass ratio
0.05%；Bacillus licheniformis powder, food stage, 00,000,000,000/g vigor, accounting for reconstituted tobacco mass ratio is 0.02%；Freeze-dried lactic acid bacteria
Powder, food stage, 200,000,000,000/g vigor, accounting for reconstituted tobacco mass ratio is 0.02%.
(2) strong acid ion macropore exchanger resin absorption：In the reconstituted tobacco extract of above-mentioned steps (1), add and account for again
Make the strong acid ion macropore exchanger resin HZ016 that quality of tobacco is than 0.1%, under the conditions of pH4.5,55 DEG C, in sorption extraction liquid
Nicotine 50min, through screen filtration remove absorption nicotine strong acid ion macroporous resin, obtain reconstituted tobacco extract；
Described strong acid ion macropore exchanger resin is food stage, particle size range 0.5～0.8mm, preferably HZ016
Deng；Screen cloth is 60 mesh, diameter 0.250mm.
(3) concentrate：It is concentrated in vacuo to solid content 40% by 75 DEG C of above-mentioned steps (2) extract, obtain improving and inhale taste product
The reproduced tobacco concentrated liquid of matter.In the process, more than 70 DEG C, the enzyme of the present invention and bacterium inactivate temperature.
(4) substrate preparation：Residue after extraction for the reconstituted tobacco, by implementing 106 DEG C of dried, inactivation with drying machine
Enzyme and bacterium, manufacture the baccy fiber of the water content being adjusted to mass ratio 11%, baccy fiber are configured to sheet and make cigarette
Careless substrate.
(5) roll：The concentrated solution of step (3) is sprayed to and on the Nicotiana tabacum L. substrate of step (4), rolls into reconstituted tobacco.
Embodiment 2
The present invention provides a kind of reconstituted tobacco of integrated approach General Promotion quality, and its preparation comprises the following steps：
(1) composite fermentation：In the extraction process of reconstituted tobacco, by the amount with the 0.2% of reconstituted tobacco mass ratio, by black fermented preparation
Mould feruloyl esterase, soil lignin peroxidase (S-Lip), bacillus licheniformis powder, freeze-dried lactic acid bacteria powder press a definite proportion
Example is added to mix homogeneously in reconstituted tobacco aqueous solution, adjusts pH to 5.0, under the conditions of 40 DEG C, ferments 4 hours；
Described aspergillus niger feruloyl esterase is food stage, enzyme activity 500u/g, and the mass ratio accounting for reconstituted tobacco is 0.08%；
Soil lignin peroxidase (S-Lip), food stage, enzyme activity 1000u/g, account for the 0.05% of reconstituted tobacco mass ratio；Lichens
Bacilluss powder, food stage, 300,000,000,000/g vigor, accounting for reconstituted tobacco mass ratio is 0.02%；Freeze-dried lactic acid bacteria powder, food stage,
100000000000/g vigor, accounting for reconstituted tobacco mass ratio is 0.05%.
(2) strong acid ion macropore exchanger resin absorption：In the reconstituted tobacco extract of above-mentioned steps (1), add and account for again
Make the strong acid ion macropore exchanger resin that quality of tobacco is than 0.2%, adjust pH5.0, the cigarette under the conditions of 60 DEG C, in sorption extraction liquid
Alkali 40min, removes the strong acid ion macroporous resin of absorption nicotine, obtains reconstituted tobacco extract through screen filtration；
Described strong acid ion macropore exchanger resin is food stage, particle size range 0.3～1.2mm, preferably HZ016
Type；Screen cloth is 60 mesh, diameter 0.250mm.
(3) concentrate：It is concentrated in vacuo to solid content 40% by 80 DEG C of above-mentioned steps (2) extract, obtain improving and inhale taste product
The reproduced tobacco concentrated liquid of matter.In the process, more than 70 DEG C, the enzyme of the present invention and bacterium inactivate temperature.
(4) substrate preparation：Residue after extraction for the reconstituted tobacco, by implementing 102 DEG C of dried, inactivation with drying machine
Enzyme and bacterium, manufacture the baccy fiber being adjusted to the water content that mass ratio is 12%, baccy fiber are configured to sheet and make
Nicotiana tabacum L. substrate.
(5) roll：The concentrated solution of step (3) is sprayed to and on the Nicotiana tabacum L. substrate of step (4), rolls into reconstituted tobacco.
Embodiment 3
The present invention provides a kind of reconstituted tobacco of integrated approach General Promotion quality, and its preparation comprises the following steps：
(1) composite fermentation：In the extraction process of reconstituted tobacco, by the amount with the 0.3% of reconstituted tobacco mass ratio, by black fermented preparation
Mould feruloyl esterase, soil lignin peroxidase (S-Lip), bacillus licheniformis powder, freeze-dried lactic acid bacteria powder press a definite proportion
Example is added to mix homogeneously in reconstituted tobacco aqueous solution, adjusts pH to 5.5, under the conditions of 37 DEG C, ferments 5 hours；
Described aspergillus niger feruloyl esterase is food stage, enzyme activity 800u/g, and the mass ratio accounting for reconstituted tobacco is 0.1%；
Soil lignin peroxidase (S-Lip), food stage, enzyme activity 1000u/g, account for the 0.1% of reconstituted tobacco mass ratio；Lichens bud
Born of the same parents' bacillus powder, food stage, 100,000,000,000/g vigor, accounting for reconstituted tobacco mass ratio is 0.05%；Freeze-dried lactic acid bacteria powder, food stage,
100000000000/g vigor, accounting for reconstituted tobacco mass ratio is 0.05%.
(2) strong acid ion macropore exchanger resin absorption：In the reconstituted tobacco extract of above-mentioned steps (1), add and account for again
Make the strong acid ion macropore exchanger resin that quality of tobacco is than 0.3%, adjust pH5, the nicotine under the conditions of 65 DEG C, in sorption extraction liquid
30min, removes the strong acid ion macroporous resin of absorption nicotine, obtains reconstituted tobacco extract through screen filtration；
Described strong acid ion macropore exchanger resin is food stage, particle size range 0.8～1.0mm, preferably HZ016
Type；Screen cloth is 80 mesh.
(3) concentrate：It is concentrated in vacuo to solid content 40% by 85 DEG C of above-mentioned steps (2) extract, obtain improving and inhale taste product
The reproduced tobacco concentrated liquid of matter.In the process, more than 70 DEG C, the enzyme of the present invention and bacterium inactivate temperature.
(4) substrate preparation：Residue after extraction for the reconstituted tobacco, by implementing 108 DEG C of dried, inactivation with drying machine
Enzyme and bacterium, manufacture and are adjusted to the baccy fiber that mass ratio is 13% water content, baccy fiber is configured to sheet and makes cigarette
Careless substrate.
(5) roll：The concentrated solution of step (3) is sprayed to and on the Nicotiana tabacum L. substrate of step (4), rolls into reconstituted tobacco.
Test example 1：Sensory evaluating smoking's Piglet s colibacillosis
The reconstituted tobacco of embodiment 1,2,3 preparation is rolled into cigarette, put in climatic chamber (22 DEG C+1, relatively wet
Spend for 60%+2%) balance 48h, asks the expert group of smokeing panel test to be smoked panel test.
Repeatedly smoke panel test through the group of smokeing panel test, table 1 shows：The present invention preparation integrated approach General Promotion quality reproduce cigarette
Leaf, enzyme and microbial hydrolysis transformation technology combine macroporous adsorption resin technology, can reduce in reconstituted tobacco extract and substrate
The macromole content such as nicotine and protein, lignin, pectin, starch, cellulose, reduces smoke pH, make production reproduces cigarette
Leaf reduces to stimulate, reduce miscellaneous QI, flue gas and suitable, General Promotion reconstituted tobacco quality.
Sensory evaluating smoking's Contrast on effect of the reconstituted tobacco of table 1. complex art General Promotion quality
Test example 2：The detection of reconstituted tobacco content of lignin
To detect the content of lignin in reconstituted tobacco using the classical way Klason method measuring content of lignin, it is former
Reason is to remove cellulose with 72% sulphuric acid, and the lignin of indissoluble is through filtering, washing, drying is weighed, calculates etc., and step can get it
Content, the content of lignin in the reconstituted tobacco of integrated approach General Promotion quality of the present invention all has reduction, result such as table 2.
The content of lignin of the reconstituted tobacco of table 2. integrated approach General Promotion quality
| Experimental group | Content of lignin | Lignin reduction rate |
| Blank | 3.45% | / |
| 1 group of embodiment | 2.89% | 16.23% |
| 2 groups of embodiment | 2.92% | 15.36% |
| 3 groups of embodiment | 2.62% | 24.05% |
Test example 3：Reconstituted tobacco nicotine content detects
Using continuous flow method, near infrared spectrum (AOTF-NIR) detects the nicotine in the reconstituted tobacco of online acquisition, knot
Fruit such as table 3, all decreases through the reconstituted tobacco nicotine content that complex art is processed.
The nicotine content of the reconstituted tobacco of table 3. integrated approach General Promotion quality
| Experimental group | Nicotine content | Reduction rate |
| Blank | 0.81% | / |
| 1 group of embodiment | 0.73% | 9.87% |
| 2 groups of embodiment | 0.71% | 12.34% |
| 3 groups of embodiment | 0.67% | 17.28% |
The above, the only specific embodiment of the present invention, but protection scope of the present invention is not limited thereto, and any
Belong to those skilled in the art the invention discloses technical scope in, the change or replacement that can readily occur in, all answer
It is included within the scope of the present invention.Therefore, protection scope of the present invention should be defined by scope of the claims.
Claims (5)
1. a kind of integrated approach of General Promotion reconstituted tobacco quality is it is characterised in that comprise the steps：
(1) composite fermentation：In the extraction process of reconstituted tobacco, by the amount with the 0.14～0.4% of reconstituted tobacco mass ratio, will be black
Aspergillosis feruloyl esterase, soil lignin peroxidase (S-Lip), bacillus licheniformis powder, freeze-dried lactic acid bacteria powder are pressed certain
Ratio is added to mix homogeneously in reconstituted tobacco aqueous solution, adjusts pH to 4.5～6.0, under the conditions of 35～45 DEG C, fermentation 1～
12 hours, obtain reconstituted tobacco extract；
(2) strong acid ion macropore exchanger resin absorption：In the reconstituted tobacco extract of above-mentioned steps (1), add to account for and reproduce cigarette
The strong acid ion macropore exchanger resin than 0.1～0.3% for the leaf quality, under the conditions of pH4.5～6.0,55～65 DEG C, sorption extraction
Nicotine 30～60min in liquid, removes the strong acid ion macroporous resin of absorption nicotine through screen filtration, obtains reconstituted tobacco extraction
Liquid；
(3) concentrate：It is concentrated in vacuo to solid content 40% by 75～85 DEG C of above-mentioned steps (2) gained reconstituted tobacco extract, obtain
To the reproduced tobacco concentrated liquid improving smoking quality, in the process, more than 70 DEG C, the enzyme of the present invention and bacterium inactivate temperature；
(4) substrate preparation：Residue after extraction for the reconstituted tobacco, by implementing 100～120 DEG C of dried with drying machine, goes out
Live enzyme and bacterium, manufactures the baccy fiber of the water content being adjusted to certain, baccy fiber is configured to sheet and makes tobacco-based
Piece.
(5) roll：The reproduced tobacco concentrated liquid of step (3) is sprayed to and on the Nicotiana tabacum L. substrate of step (4), rolls into reconstituted tobacco.
2. General Promotion reconstituted tobacco quality as claimed in claim 1 integrated approach it is characterised in that：Institute in step (1)
The aspergillus niger feruloyl esterase stated be food stage, enzyme activity 100-1000u/g, account for reconstituted tobacco mass ratio be 0.05～
0.15%；Soil lignin peroxidase (S-Lip), food stage, enzyme activity 100～3000u/g, account for reconstituted tobacco mass ratio
0.05～0.15%；Bacillus licheniformis powder, food stage, 100,000,000,000～500,000,000,000/g vigor, accounting for reconstituted tobacco mass ratio is
0.02～0.05%；Freeze-dried lactic acid bacteria powder, food stage, 100,000,000,000～200,000,000,000/g vigor, accounting for reconstituted tobacco mass ratio is 0.02
～0.05%.
3. General Promotion reconstituted tobacco quality as claimed in claim 1 integrated approach it is characterised in that：Institute in step (2)
The strong acid ion macropore exchanger resin stated is food stage, particle size range 0.3～1.2mm, preferably HZ016 etc.；Screen cloth is 60
Mesh～120 mesh, diameter 0.125mm～0.250mm.
4. General Promotion reconstituted tobacco quality as claimed in claim 1 integrated approach it is characterised in that：Institute in step (4)
The certain water content stated is mass ratio 5～15%.
5. General Promotion reconstituted tobacco quality as claimed in claim 1 integrated approach it is characterised in that：Institute in step (4)
The certain water content stated is mass ratio 11～13%.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2069814A (en) * | 1980-02-27 | 1981-09-03 | Inst Przemyslu Fermentacyjnego | Microbiological treatment of tobacco and forming reconstituted tobacco or substitute |
| CN101613980A (en) * | 2009-07-18 | 2009-12-30 | 大庆天成生物纤维制品有限责任公司 | A kind of biological assistant of grass as raw material cleaning pulping |
| CN104957752A (en) * | 2015-07-09 | 2015-10-07 | 川渝中烟工业有限责任公司 | Biological treatment method of papermaking-method reconstituted tobacco raw materials and pulp |
| CN104957758A (en) * | 2015-07-09 | 2015-10-07 | 川渝中烟工业有限责任公司 | Method for improving sensory quality of tobacco reconstituted through papermaking method |
| CN105852189A (en) * | 2016-05-31 | 2016-08-17 | 湖北中烟工业有限责任公司 | Method for preparing tobacco extract by utilizing waste and inferior tobacco leaves |
-
2016
- 2016-08-31 CN CN201610781804.9A patent/CN106387981B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2069814A (en) * | 1980-02-27 | 1981-09-03 | Inst Przemyslu Fermentacyjnego | Microbiological treatment of tobacco and forming reconstituted tobacco or substitute |
| CN101613980A (en) * | 2009-07-18 | 2009-12-30 | 大庆天成生物纤维制品有限责任公司 | A kind of biological assistant of grass as raw material cleaning pulping |
| CN104957752A (en) * | 2015-07-09 | 2015-10-07 | 川渝中烟工业有限责任公司 | Biological treatment method of papermaking-method reconstituted tobacco raw materials and pulp |
| CN104957758A (en) * | 2015-07-09 | 2015-10-07 | 川渝中烟工业有限责任公司 | Method for improving sensory quality of tobacco reconstituted through papermaking method |
| CN105852189A (en) * | 2016-05-31 | 2016-08-17 | 湖北中烟工业有限责任公司 | Method for preparing tobacco extract by utilizing waste and inferior tobacco leaves |
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