CN110742302B - Cigarette capable of reducing free radical content in smoke and preparation method thereof - Google Patents
Cigarette capable of reducing free radical content in smoke and preparation method thereof Download PDFInfo
- Publication number
- CN110742302B CN110742302B CN201911088207.8A CN201911088207A CN110742302B CN 110742302 B CN110742302 B CN 110742302B CN 201911088207 A CN201911088207 A CN 201911088207A CN 110742302 B CN110742302 B CN 110742302B
- Authority
- CN
- China
- Prior art keywords
- cigarette
- extract
- polygonatum
- filter
- drying
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
- A24B15/18—Treatment of tobacco products or tobacco substitutes
- A24B15/24—Treatment of tobacco products or tobacco substitutes by extraction; Tobacco extracts
- A24B15/26—Use of organic solvents for extraction
-
- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B3/00—Preparing tobacco in the factory
- A24B3/12—Steaming, curing, or flavouring tobacco
-
- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24D—CIGARS; CIGARETTES; TOBACCO SMOKE FILTERS; MOUTHPIECES FOR CIGARS OR CIGARETTES; MANUFACTURE OF TOBACCO SMOKE FILTERS OR MOUTHPIECES
- A24D1/00—Cigars; Cigarettes
- A24D1/002—Cigars; Cigarettes with additives, e.g. for flavouring
-
- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24D—CIGARS; CIGARETTES; TOBACCO SMOKE FILTERS; MOUTHPIECES FOR CIGARS OR CIGARETTES; MANUFACTURE OF TOBACCO SMOKE FILTERS OR MOUTHPIECES
- A24D3/00—Tobacco smoke filters, e.g. filter-tips, filtering inserts; Filters specially adapted for simulated smoking devices; Mouthpieces for cigars or cigarettes
- A24D3/02—Manufacture of tobacco smoke filters
- A24D3/0204—Preliminary operations before the filter rod forming process, e.g. crimping, blooming
- A24D3/0212—Applying additives to filter materials
- A24D3/022—Applying additives to filter materials with liquid additives, e.g. application of plasticisers
Abstract
The invention belongs to the technical field of cigarettes, and particularly relates to a cigarette capable of reducing the content of free radicals in smoke and a preparation method thereof. According to the cigarette, the extract obtained by alcohol extraction, enzymolysis and water extraction of the polygonatum odoratum is compounded with the carboxymethyl chitosan oligosaccharide and then added into tobacco shreds and/or filter tips, so that the stability of the polygonatum odoratum extract can be remarkably improved, and the effects of remarkably improving the clearance rate of free radicals and improving the smoking quality of the cigarette are achieved; meanwhile, the cigarette preparation method has simple steps and is suitable for industrial large-scale production.
Description
Technical Field
The invention belongs to the technical field of cigarettes. More particularly, relates to a cigarette for reducing the content of free radicals in smoke and a preparation method thereof.
Background
The cigarette smoke contains thousands of compounds, free radicals are one of the compounds, most of the free radicals are harmful to human bodies, can directly damage cell membranes and biological macromolecules, can consume antioxidant enzymes in human bodies, causes the disturbance of antioxidant enzyme systems and the reduction of enzyme activity in the human bodies, and is a main reason for the increase of the incidence of diseases such as respiratory diseases, cardiovascular diseases, oral diseases, tumors and the like.
At present, particulate free radicals with larger molecular weight and higher stability in smoke are removed mainly by methods of improving the interception and adsorption efficiency of a filter tip, increasing the air permeability of cigarette paper and the like; gas-phase free radicals with small weight and poor stability in smoke are removed mainly by adding a free radical inhibitor, a free radical scavenger, a natural antioxidant, a traditional Chinese medicine extract, trace elements, natural minerals and the like. For example, Chinese patent application CN102423125A discloses a tobacco product containing vitamin C compounds and its application, the tobacco product has more vitamin C in the main stream smoke by adding a certain amount of vitamin C compounds, thereby achieving the purpose of reducing free radicals in the smoke, and simultaneously reducing the pungent taste of the cigarette, but the vitamin C antioxidants have poor stability, are very easy to be naturally oxidized, and the ability of removing free radicals after long-time storage can be obviously reduced.
Therefore, the cigarette which has strong stability and can obviously reduce the free radical content of the smoke and the preparation method thereof are urgently needed.
Disclosure of Invention
The invention aims to solve the technical problem of overcoming the defect and the defect of poor stability of adding an antioxidant into cigarettes in the prior art and providing the cigarettes with strong stability and capability of obviously reducing the content of free radicals in smoke.
The invention also aims to provide a preparation method of the cigarette.
The above purpose of the invention is realized by the following technical scheme:
a cigarette for reducing free radical content in smoke is prepared by adding cigarette additive comprising rhizoma Polygonati Odorati extract and carboxymethyl chitosan oligosaccharide in an amount of 1 (0.2-2) into tobacco shred and/or filter tip of cigarette;
wherein the preparation method of the polygonatum odoratum extract comprises the following steps:
s1, drying and crushing polygonatum odoratum into fine powder, adding an ethanol solution with the concentration of 80-100% (v/v), carrying out ultrasonic extraction for 15-60 min, filtering to obtain filter residues, concentrating the filtrate, and drying to obtain an alcohol extract of the polygonatum odoratum;
s2, adding the filter residue obtained in the step S1 into water, adding a compound enzyme, and carrying out enzymolysis for 15-60 min at the temperature of 25-30 ℃ and under the condition that the pH value is 5-7 to obtain an enzymolysis liquid;
s3, carrying out reflux extraction on the enzymolysis liquid obtained in the step S2 for 30-90 min, filtering, adding absolute ethyl alcohol into filtrate, shaking and mixing, precipitating at 1-4 ℃ for 8-16 h, centrifuging and drying to obtain a polygonatum aqueous extract;
and S4, mixing the alcohol extract of the polygonatum obtained in the step S1 with the water extract of the polygonatum obtained in the step S3 to obtain the polygonatum.
According to the invention, the extract of polygonatum odoratum after alcohol extraction, enzymolysis and water extraction is added into tobacco shreds and/or filter tips, the polygonatum odoratum extract contains active ingredients such as flavonoids and polysaccharides, so that the content of free radicals in smoke can be remarkably reduced, and volatile fragrant substances can improve the smoking quality of cigarettes. However, the active ingredients in the polygonatum odoratum extract are unstable and are very easily affected by the external environment during the standing process, and after a period of time, the clearance rate of free radicals is remarkably reduced, and the stability is low. Aiming at the problems, the inventor surprisingly discovers that the polygonatum odoratum extract and the carboxymethyl chitosan oligosaccharide are compounded to be used as a cigarette additive to be added into cigarette tobacco shreds and/or filter tips, so that the stability of the polygonatum odoratum extract can be remarkably improved, and the free radical clearance rate can reach about 20% after the cigarette is placed for 12 months. The inventor believes that the mechanism of the method is that the carboxymethyl chitosan oligosaccharide forms a layer of protective film outside the active ingredients of the polygonatum extract and is combined with the active ingredients through hydrogen bonds to achieve the effect of stabilizing the active ingredients of the polygonatum extract.
Preferably, the cigarette additive consists of a polygonatum extract and carboxymethyl chitosan oligosaccharide in a ratio of 1 (0.2-1).
More preferably, the cigarette additive consists of the polygonatum extract and the carboxymethyl chitosan oligosaccharide in a ratio of 1: 0.5.
Further, in the step S2, the complex enzyme is composed of papain and cellulase in a weight ratio of 1 (1-5).
Preferably, in the step S2, the complex enzyme is composed of papain and cellulase in a weight ratio of 1 (1-3).
More preferably, in the step S2, the complex enzyme consists of papain and cellulase in a weight ratio of 1: 3.
Furthermore, in the step S2, the addition amount of the complex enzyme is 4-8 g/L.
Preferably, in the step S2, the addition amount of the complex enzyme is 4-6 g/L.
More preferably, in the step S2, the addition amount of the complex enzyme is 5 g/L.
When water extraction is carried out on polygonatum, due to the existence of certain viscous components in polygonatum, filter paper is easy to block during filtration, so that the problems of slow filtration and low polysaccharide extraction efficiency are caused. The inventor finds that the filter residue after alcohol extraction of the polygonatum odoratum is subjected to enzymolysis by using the complex enzyme and then subjected to water extraction, so that the problems of filter blockage and slowness can be avoided, the cell wall of the polygonatum odoratum is damaged, the release of active ingredients is promoted, and the extraction rate of the active ingredients of the polygonatum odoratum is remarkably improved.
Further, in the step S1, the power of ultrasonic extraction is 100-500W.
Preferably, in the step S1, the power of the ultrasonic extraction is 200-500W.
More preferably, in step s1, the power of the ultrasonic extraction is 300W.
Furthermore, in the step S3, the temperature of the reflux extraction is 70-85 ℃.
Preferably, in the step S3, the temperature of the reflux extraction is 70-80 ℃.
More preferably, in step s3, the temperature of the reflux extraction is 80 ℃.
Further, in the step s1, the addition amount of the ethanol solution is 5 to 20 times (weight to volume ratio) of the weight of the polygonatum fine powder.
Furthermore, in the step S2, the addition amount of the water is 5-20 times (weight to volume ratio) of the weight of the filter residue.
Further, in the step S3, the addition amount of the absolute ethyl alcohol is 4-6 times (volume ratio) of the volume of the filtrate.
Furthermore, the adding amount of the cigarette additive in the cut tobacco is 0.02-1% of the weight of the cut tobacco.
Preferably, the addition amount of the cigarette additive in the cut tobacco is 0.02-0.5% of the weight of the cut tobacco.
More preferably, the addition amount of the cigarette additive in the cut tobacco is 0.2% of the weight of the cut tobacco.
Further, the adding amount of the cigarette additive in the filter tip is 1-10% of the weight of the filter tip.
Preferably, the adding amount of the cigarette additive in the filter tip is 1-5% of the weight of the filter tip.
More preferably, the cigarette additive is added into the filter in an amount of 2% by weight of the filter.
In addition, the invention also provides a preparation method of the cigarette, which comprises the following steps:
preparing a polygonatum extract, adding the obtained polygonatum extract and carboxymethyl chitosan oligosaccharide into water, dissolving to prepare an aqueous solution containing 0.1-1% of a cigarette additive, adding the aqueous solution into cigarette tobacco shreds and/or filter tips, drying, balancing, and making cigarettes.
The invention has the following beneficial effects:
(1) according to the cigarette for reducing the content of free radicals in smoke, the extract obtained by alcohol extraction, enzymolysis and water extraction of polygonatum odoratum is compounded with carboxymethyl chitosan oligosaccharide and added into tobacco shreds and/or filter tips, so that the stability of the polygonatum odoratum extract can be remarkably improved, and the effects of remarkably improving the clearance rate of the free radicals and improving the smoking quality of the cigarette are achieved.
(2) The preparation method of the cigarette for reducing the free radical content in the smoke has simple steps and can be applied to industrial large-scale production.
Detailed Description
The present invention is further illustrated by the following specific examples, which are not intended to limit the invention in any way. Reagents, methods and apparatus used in the present invention are conventional in the art unless otherwise indicated.
Unless otherwise indicated, reagents and materials used in the following examples are commercially available.
Example 1A cigarette having reduced free radical content in Smoke
The cigarette with low harmful components in smoke is prepared by the following method:
s1, drying and crushing polygonatum odoratum into fine powder, adding 20kg of fine powder into 85% (v/v) ethanol solution with the weight-volume ratio of 1:10, carrying out ultrasonic extraction for 30min at 300W, filtering to obtain 25kg of filter residue, concentrating and drying the filtrate to obtain 697g of polygonatum odoratum alcohol extract;
s2, adding 25kg of filter residue obtained in the step S1 into water with the weight-volume ratio of 1:15, adding 5g/L of complex enzyme consisting of papain and cellulase according to the weight-volume ratio of 1:3, and carrying out enzymolysis for 30min at the temperature of 27 ℃ and under the condition that the pH value is 6 to obtain an enzymolysis liquid;
s3, carrying out reflux extraction on the enzymolysis liquid obtained in the step S2 at 80 ℃ for 60min, filtering, adding absolute ethyl alcohol with the volume ratio of 1:4 into the filtrate, shaking and mixing, precipitating at 4 ℃ for 12h, centrifuging and drying to obtain 2692g of polygonatum odoratum aqueous extract;
s4, mixing the alcohol extract of the polygonatum obtained in the step S1 with the water extract of the polygonatum obtained in the step S3 to obtain 3389g of the polygonatum extract;
s5, adding 3389g of the polygonatum odoratum extract obtained in the step S4 and 1694.5g of carboxymethyl chitosan oligosaccharide into water, dissolving to prepare an aqueous solution containing 0.5% of a cigarette additive, adding the aqueous solution into the tobacco shreds and the filter tip according to the weight of 0.2% of the tobacco shreds and 2% of the filter tip respectively, drying, balancing and preparing cigarettes.
Example 2A cigarette having reduced free radical content in Smoke
The cigarette with low harmful components in smoke is prepared by the following method:
s1, drying and crushing polygonatum odoratum into fine powder, adding 20kg of fine powder into 85% (v/v) ethanol solution with the weight-volume ratio of 1:10, carrying out ultrasonic extraction for 30min at 300W, filtering to obtain 26kg of filter residue, concentrating and drying the filtrate to obtain 654g of polygonatum odoratum alcohol extract;
s2, adding 26kg of filter residue obtained in the step S1 into water with the weight-volume ratio of 1:15, adding 5g/L of complex enzyme consisting of papain and cellulase according to the weight-volume ratio of 1:3, and carrying out enzymolysis for 30min at the temperature of 27 ℃ and under the condition that the pH value is 6 to obtain an enzymolysis liquid;
s3, carrying out reflux extraction on the enzymolysis liquid obtained in the step S2 at 80 ℃ for 60min, filtering, adding absolute ethyl alcohol with the volume ratio of 1:4 into the filtrate, shaking and mixing, precipitating at 4 ℃ for 12h, centrifuging and drying to obtain 2674g of polygonatum odoratum aqueous extract;
s4, mixing the alcohol extract of the polygonatum obtained in the step S1 with the water extract of the polygonatum obtained in the step S3 to obtain 3328g of the polygonatum extract;
s5, adding 3328g of the polygonatum odoratum extract obtained in the step S4 and 3328g of carboxymethyl chitosan oligosaccharide into water, dissolving to prepare an aqueous solution containing 0.5% of a cigarette additive, adding the aqueous solution into the tobacco shreds and the filter tip according to the weight of 0.2% of the tobacco shreds and 2% of the filter tip respectively, drying, balancing and preparing cigarettes.
Example 3A cigarette having reduced free radical content in Smoke
The cigarette with low harmful components in smoke is prepared by the following method:
s1, drying and crushing polygonatum odoratum into fine powder, adding 20kg of fine powder into 85% (v/v) ethanol solution with the weight-volume ratio of 1:10, carrying out ultrasonic extraction for 30min at 300W, filtering to obtain 25kg of filter residue, concentrating the filtrate, and drying to obtain 684g of polygonatum odoratum alcohol extract;
s2, adding 25kg of filter residue obtained in the step S1 into water with the weight-volume ratio of 1:15, adding 5g/L of complex enzyme consisting of papain and cellulase according to the weight-volume ratio of 1:3, and carrying out enzymolysis for 30min at the temperature of 27 ℃ and under the condition that the pH value is 6 to obtain an enzymolysis liquid;
s3, carrying out reflux extraction on the enzymolysis liquid obtained in the step S2 at 80 ℃ for 60min, filtering, adding absolute ethyl alcohol with the volume ratio of 1:4 into the filtrate, shaking and mixing, precipitating at 4 ℃ for 12h, centrifuging and drying to obtain 2653g of polygonatum odoratum aqueous extract;
s4, mixing the alcohol extract of the polygonatum obtained in the step S1 with the water extract of the polygonatum obtained in the step S3 to obtain 3337g of the polygonatum extract;
s5, adding 3337g of the polygonatum odoratum extract obtained in the step S4 and 667.4g of carboxymethyl chitosan oligosaccharide into water, dissolving to prepare an aqueous solution containing 0.5% of a cigarette additive, adding the aqueous solution into the tobacco shreds and the filter tip according to the weight of 0.2% of the tobacco shreds and 2% of the filter tip respectively, drying, balancing and preparing cigarettes.
Example 4A cigarette having reduced free radical content in Smoke
The difference from example 1 is that example 4 only cigarette additives were added to tobacco shreds, no filter was added, and the rest of the parameters and operation refer to example 1.
Example 5A cigarette having reduced free radical content in Smoke
The cigarette with low harmful components in smoke is prepared by the following method:
s1, drying and crushing polygonatum odoratum into fine powder, adding 100kg of fine powder into an ethanol solution with the weight volume ratio of 1:20 and the concentration of 80% (v/v), carrying out 100W ultrasonic extraction for 60min, filtering to obtain 138kg of filter residue, concentrating and drying the filtrate to obtain 3.5kg of polygonatum odoratum alcohol extract;
s2, adding 138kg of filter residues obtained in the step S1 into water with the weight-volume ratio of 1:5, adding 4g/L of complex enzyme consisting of papain and cellulase according to the weight-volume ratio of 1:5, and carrying out enzymolysis for 60min at the temperature of 30 ℃ under the condition that the pH value is 5 to obtain an enzymolysis liquid;
s3, carrying out reflux extraction on the enzymolysis liquid obtained in the step S2 at 70 ℃ for 90min, filtering, adding absolute ethyl alcohol with the volume ratio of 1:6 into the filtrate, shaking and mixing, precipitating at 1 ℃ for 8h, centrifuging and drying to obtain 13.4kg of polygonatum odoratum aqueous extract;
s4, mixing the alcohol extract of the polygonatum obtained in the step S1 with the water extract of the polygonatum obtained in the step S3 to obtain 16.9kg of the polygonatum extract;
s5, adding the 16.9kg of polygonatum odoratum extract obtained in the step S4 and 8.45kg of carboxymethyl chitosan oligosaccharide into water, dissolving to prepare an aqueous solution containing 0.1% of cigarette additives, adding the aqueous solution into the cut tobacco according to the weight of the cigarette additives being 0.02% of the weight of the cut tobacco, drying, balancing, and making cigarettes.
Example 6A cigarette having reduced free radical content in Smoke
The cigarette with low harmful components in smoke is prepared by the following method:
s1, drying and crushing polygonatum odoratum into fine powder, adding 100kg of fine powder into 100% (v/v) ethanol solution with the weight-volume ratio of 1:5, carrying out ultrasonic extraction for 15min at 500W, filtering to obtain 130kg of filter residue, concentrating and drying the filtrate to obtain 3.1kg of polygonatum odoratum alcohol extract;
s2, adding 130kg of filter residues obtained in the step S1 into water with the weight-volume ratio of 1:20, adding 8g/L of complex enzyme consisting of papain and cellulase according to the weight-volume ratio of 1:1, and carrying out enzymolysis for 15min at the temperature of 25 ℃ under the condition that the pH value is 7 to obtain an enzymolysis liquid;
s3, carrying out reflux extraction on the enzymolysis liquid obtained in the step S2 at 85 ℃ for 30min, filtering, adding absolute ethyl alcohol with the volume ratio of 1:5 into the filtrate, shaking and mixing, precipitating for 16h at 3 ℃, centrifuging and drying to obtain 13.2kg of polygonatum odoratum aqueous extract;
s4, mixing the alcohol extract of the polygonatum obtained in the step S1 with the water extract of the polygonatum obtained in the step S3 to obtain 16.3kg of polygonatum extract;
s5, adding the 16.3kg of polygonatum odoratum extract obtained in the step S4 and 8.15kg of carboxymethyl chitosan oligosaccharide into water, dissolving to prepare an aqueous solution containing 1% of the cigarette additive, adding the aqueous solution into the filter according to the weight of the cigarette additive accounting for 5% of the weight of the filter, drying, balancing, and preparing cigarettes.
Comparative example 1 cigarette
The difference from example 1 is that the cigarette of comparative example 1 is not added with cigarette additives, and the rest parameters and operation refer to example 1.
Comparative example 2 cigarette
The difference from example 1 is that the cigarette additive of comparative example 2 is polygonatum odoratum extract, which does not contain carboxymethyl chitosan oligosaccharide, and the rest parameters and operation refer to example 1.
Comparative example 3 cigarette
The difference from example 1 is that carboxymethyl chitosan oligosaccharide was replaced with chitosan oligosaccharide in the cigarette additive of comparative example 3, and the rest parameters and operation refer to example 1.
Comparative example 4 cigarette
The difference from example 1 is that the extract of polygonatum odoratum of comparative example 4 is not subjected to enzymatic hydrolysis, and the specific extraction method comprises the following steps:
s1, drying and crushing polygonatum odoratum into fine powder, adding 10kg of fine powder into 85% (v/v) ethanol solution with the weight-volume ratio of 1:10, carrying out ultrasonic extraction for 30min at 300W, filtering to obtain 12kg of filter residue, concentrating and drying the filtrate to obtain 346g of polygonatum odoratum alcohol extract;
s2, adding 12kg of filter residues obtained in the step S1 into water with the weight-volume ratio of 1:15, carrying out reflux extraction at 80 ℃ for 60min, filtering, adding absolute ethyl alcohol with the volume ratio of 1:4 into the filtrate, carrying out shaking mixing, carrying out precipitation at 4 ℃ for 12h, centrifuging and drying to obtain 648g of the polygonatum odoratum water extract;
s3, mixing the alcohol extract of the polygonatum obtained in the step S1 with the water extract of the polygonatum obtained in the step S2 to obtain 994g of the polygonatum extract;
the remaining parameters and operations refer to example 1.
Comparative example 5 cigarette
The difference from the example 1 is that the complex enzyme added in the preparation process of the polygonatum odoratum extract in the comparative example 5 is changed into papain, and the rest parameters and operation are referred to the example 1.
Comparative example 6 cigarette
The difference from the example 1 is that the polygonatum odoratum extract of the comparative example 6 is only subjected to alcohol extraction, and the specific extraction method comprises the following steps:
drying rhizoma Polygonati Odorati, pulverizing into fine powder, adding 10kg of fine powder into 85% (v/v) ethanol solution at a weight-volume ratio of 1:10, ultrasonic extracting for 30min at 300W, filtering to obtain 13kg of residue, concentrating the filtrate, and drying to obtain 351g of rhizoma Polygonati Odorati extract;
the remaining parameters and operations refer to example 1.
Comparative example 7 cigarette
The difference from example 1 is that the polygonatum odoratum extract of comparative example 7 is only subjected to water extraction, and the specific extraction method comprises the following steps:
drying rhizoma Polygonati Odorati, pulverizing into fine powder, adding 10kg of fine powder into water at a weight volume ratio of 1:15, extracting under reflux at 80 deg.C for 60min, filtering, adding anhydrous ethanol at a volume ratio of 1:4 into the filtrate, shaking, mixing, precipitating at 4 deg.C for 12h, centrifuging, and drying to obtain 701g rhizoma Polygonati Odorati water extract;
the remaining parameters and operations refer to example 1.
Experimental example 1 determination of cigarette radical clearance and stability
The free radical clearance of the cigarettes prepared in examples 1 to 6 and comparative examples 2 to 7 is measured by taking the comparative example 1 as a control, and the free radical clearance of each group of cigarettes is measured after the cigarettes are placed for 3, 6 and 12 months under the conditions of 25 +/-2 ℃ and 60 +/-5% of humidity, and the results are shown in table 1.
TABLE 1 determination of cigarette free radical scavenging rate and stability
As can be seen from table 1:
(1) the cigarettes prepared by the embodiments 1-3 of the invention have high free radical clearance rate which is over 28 percent, and have no obvious change after being placed for 12 months, and have good stability; examples 4-6 (cigarette additives are added only to tobacco shreds or filter tips) the free radical clearance of the cigarettes is reduced to a certain extent, and the change is not obvious after the cigarettes are placed for 12 months;
(2) the invention has higher free radical clearance rate in comparison with 2-3 (removing or replacing carboxymethyl chitosan oligosaccharide) cigarettes in 0 month, but the free radical clearance rate is obviously reduced after the cigarettes are placed for 12 months; the cigarettes of comparative examples 4-7 have low free radical clearance rate in 0 month, and the free radical clearance rate is below 10% after the cigarettes are placed for 12 months.
Experimental example 2 evaluation of smoking quality of cigarette
Reference is made to GB5606.4-2005 cigarette part 4: sensory technical requirements, the sensory quality of the cigarettes prepared in examples 1 to 6 and comparative examples 1 to 7 was evaluated, wherein the first-class score was not less than 85, the second-class and third-class scores were not less than 75, and the fourth-class and fifth-class scores were not less than 60, and the results are shown in table 2.
TABLE 2 sensory evaluation results of cigarettes
| Group of | Scoring |
| Example 1 | 93.4 |
| Example 2 | 92.1 |
| Example 3 | 92.9 |
| Example 4 | 90.3 |
| Example 5 | 88.5 |
| Example 6 | 91.3 |
| Comparative example 1 | 76.7 |
| Comparative example 2 | 79.6 |
| Comparative example 3 | 80.2 |
| Comparative example 4 | 81.2 |
| Comparative example 5 | 83.5 |
| Comparative example 6 | 77.8 |
| Comparative example 7 | 78.4 |
As can be seen from Table 2, the sensory quality evaluation scores of the cigarettes prepared in the embodiments 1 to 6 of the invention are all more than 85 points, and the cigarettes are one type of cigarettes and are deeply favored by consumers; the cigarettes prepared in the comparative examples 1 to 7 have different degrees of problems in terms of luster, aroma, harmony, offensive odor, irritation and aftertaste, so that the scores are remarkably reduced.
The above embodiments are preferred embodiments of the present invention, but the present invention is not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention should be construed as equivalents thereof, and all such changes, modifications, substitutions, combinations, and simplifications are intended to be included in the scope of the present invention.
Claims (9)
1. A cigarette for reducing the content of free radicals in smoke is characterized in that a cigarette additive consisting of a polygonatum extract and carboxymethyl chitosan oligosaccharide in a ratio of 1 (0.2-2) is added into cigarette tobacco shreds and/or a filter tip;
wherein the preparation method of the polygonatum odoratum extract comprises the following steps:
s1, drying and crushing polygonatum odoratum into fine powder, adding an ethanol solution with the concentration of 80-100% (v/v), carrying out ultrasonic extraction for 15-60 min, filtering to obtain filter residues, concentrating the filtrate, and drying to obtain an alcohol extract of the polygonatum odoratum;
s2, adding the filter residue obtained in the step S1 into water, adding a compound enzyme, and carrying out enzymolysis for 15-60 min at the temperature of 25-30 ℃ and under the condition that the pH value is 5-7 to obtain an enzymolysis liquid;
s3, carrying out reflux extraction on the enzymolysis liquid obtained in the step S2 for 30-90 min, filtering, adding absolute ethyl alcohol into filtrate, shaking and mixing, precipitating at 1-4 ℃ for 8-16 h, centrifuging and drying to obtain a polygonatum aqueous extract;
s4, mixing the alcohol extract of the polygonatum obtained in the step S1 with the water extract of the polygonatum obtained in the step S3 to obtain the polygonatum extract;
in the step S2, the complex enzyme consists of papain and cellulase in a weight ratio of 1 (1-5).
2. The cigarette as claimed in claim 1, wherein the cigarette additive comprises polygonatum odoratum extract and carboxymethyl chitosan oligosaccharide in a ratio of 1 (0.2-1).
3. The cigarette according to claim 2, wherein the cigarette additive consists of polygonatum odoratum extract and carboxymethyl chitosan oligosaccharide in a ratio of 1: 0.5.
4. The cigarette according to claim 1, wherein in step S2, the amount of the complex enzyme is 4-8 g/L.
5. The cigarette according to claim 1, wherein in the step S1, the power of the ultrasonic extraction is 100-500W.
6. The cigarette according to claim 1, wherein in step S3, the temperature of the reflux extraction is 70-85 ℃.
7. The cigarette according to claim 1, wherein the additive is added in the tobacco shreds in an amount of 0.02-1% by weight of the tobacco shreds.
8. The cigarette according to claim 1, wherein the additive amount of the cigarette additive in the filter is 1-10% of the weight of the filter.
9. A method for preparing a cigarette according to any one of claims 1 to 8, comprising the steps of:
preparing a polygonatum extract, adding the obtained polygonatum extract and carboxymethyl chitosan oligosaccharide into water, dissolving to prepare an aqueous solution containing 0.1-1% of a cigarette additive, adding the aqueous solution into cigarette tobacco shreds and/or filter tips, drying, balancing, and making cigarettes.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911088207.8A CN110742302B (en) | 2019-11-08 | 2019-11-08 | Cigarette capable of reducing free radical content in smoke and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911088207.8A CN110742302B (en) | 2019-11-08 | 2019-11-08 | Cigarette capable of reducing free radical content in smoke and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN110742302A CN110742302A (en) | 2020-02-04 |
| CN110742302B true CN110742302B (en) | 2020-09-22 |
Family
ID=69282686
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201911088207.8A Active CN110742302B (en) | 2019-11-08 | 2019-11-08 | Cigarette capable of reducing free radical content in smoke and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110742302B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111418885B (en) * | 2020-05-20 | 2021-12-21 | 云南如茨生物科技有限公司 | Heating cigarette product |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101627834A (en) * | 2007-09-28 | 2010-01-20 | 川渝中烟工业公司 | Method for reducing content of phenolic compounds in cigarette main stream smoke gas by using chrysanthemum, Artemisia leaf and polygonatum |
| CN102408944A (en) * | 2010-09-20 | 2012-04-11 | 湖北中烟工业有限责任公司 | Tobacco additive capable of reducing irritation and increasing tobacco fragrance and preparation method thereof |
| CN104188090A (en) * | 2014-09-12 | 2014-12-10 | 湖北中烟工业有限责任公司 | Method for reducing carbonic oxide emission of paper-making reconstituted tobacco |
| CN105919158A (en) * | 2016-05-27 | 2016-09-07 | 阜阳卷烟材料厂 | Cigarette filter stick capable of reducing harmful ingredients in cigarette |
| CN108359023A (en) * | 2018-03-02 | 2018-08-03 | 北京国康本草物种生物科学技术研究院有限公司 | A kind of extracting method and fragrant solomonseal rhizome polyoses extract of fragrant solomonseal rhizome polyoses |
-
2019
- 2019-11-08 CN CN201911088207.8A patent/CN110742302B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101627834A (en) * | 2007-09-28 | 2010-01-20 | 川渝中烟工业公司 | Method for reducing content of phenolic compounds in cigarette main stream smoke gas by using chrysanthemum, Artemisia leaf and polygonatum |
| CN102408944A (en) * | 2010-09-20 | 2012-04-11 | 湖北中烟工业有限责任公司 | Tobacco additive capable of reducing irritation and increasing tobacco fragrance and preparation method thereof |
| CN104188090A (en) * | 2014-09-12 | 2014-12-10 | 湖北中烟工业有限责任公司 | Method for reducing carbonic oxide emission of paper-making reconstituted tobacco |
| CN105919158A (en) * | 2016-05-27 | 2016-09-07 | 阜阳卷烟材料厂 | Cigarette filter stick capable of reducing harmful ingredients in cigarette |
| CN108359023A (en) * | 2018-03-02 | 2018-08-03 | 北京国康本草物种生物科学技术研究院有限公司 | A kind of extracting method and fragrant solomonseal rhizome polyoses extract of fragrant solomonseal rhizome polyoses |
Non-Patent Citations (3)
| Title |
|---|
| 壳聚糖及其改性材料在降低卷烟烟气有害成分上的应用;吴艾璟等;《广东化工》;20190815;第46卷(第15期);第125页 * |
| 浙江源应用于卷烟保润植物的选择研究;杨君等;《浙江林业科技》;20110915;第31卷(第5期);第63页 * |
| 湘玉竹高效栽培技术与主要功能成分优化提取工艺研究;熊礼平;《中国优秀硕士学位论文全文数据库•农业科技辑》;20111215(第S1期);第46-60页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN110742302A (en) | 2020-02-04 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102191130B (en) | Preparation method of fermented cigarette fructus momordicae extractum | |
| CN105852189B (en) | A kind of method that utilization waste/hypo-tobacco leaf prepares tobacco extract | |
| CN105686069B (en) | A kind of tobacco extract and preparation method thereof and its purposes | |
| CN106036982B (en) | A kind of preparation method and applications of cigarette bay leaf extract | |
| CN105212262A (en) | A kind of preparation method of tobacco flavor material | |
| CN102161935A (en) | Endogenous perfume from tobacco as well as preparation method and application thereof | |
| CN103892436B (en) | A kind of improve tobacco material method of service efficiency in reconstituted tobacco produces | |
| CN101693745A (en) | Lucid ganoderma polysaccharide and extraction method thereof as well as cigarette containing lucid ganoderma polysaccharide and preparation method thereof | |
| CN102885397A (en) | Application of astragalus extract in stem strings or reproduced tobaccos | |
| CN104152268A (en) | Tobacco perfume material prepared by compound enzyme enzymolysis coupling Maillard reaction, and preparation method and application of tobacco perfume material | |
| CN102796204A (en) | Extraction method of tremella polysaccharide and application of tremella polysaccharide in tobaccos | |
| CN110742302B (en) | Cigarette capable of reducing free radical content in smoke and preparation method thereof | |
| CN108158030B (en) | Extraction method of compound traditional Chinese medicine extract and application of compound traditional Chinese medicine extract in cigarettes | |
| CN105029687A (en) | Preparing method for cigarette addition agent rich in barley polysaccharide | |
| CN103881815A (en) | Black plum extract, preparation method for same and application of extract to cigarette | |
| CN102960853B (en) | Method of utilizing ammoniation technology to improve quality of tobacco stalk extracting solution | |
| CN107485048B (en) | A kind of extracting method of banana aldehyde and its application in cigarette | |
| CN101461561B (en) | Conversion agent of fire-cured tobacco odor type as well as preparation method and use method thereof | |
| CN107802025B (en) | Preparation method of ginkgo leaf extract and application of ginkgo leaf extract in cigarettes | |
| CN102726823B (en) | A kind of method reducing tobacco sheets by paper making method extract pectic substance content | |
| CN104497163A (en) | Korla pear polysaccharide extracting method and method for applying korla pear polysaccharide to cigarettes | |
| CN113057369A (en) | Paper filter stick and preparation method thereof | |
| CN107216947A (en) | A kind of preparation method of cigarette Catechu extract | |
| CN102793263A (en) | Extraction method of tremella water extract concentrate and application of concentrate in tobaccos | |
| CN109527641B (en) | Tobacco extract and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |