CN104152268A - Tobacco perfume material prepared by compound enzyme enzymolysis coupling Maillard reaction, and preparation method and application of tobacco perfume material - Google Patents
Tobacco perfume material prepared by compound enzyme enzymolysis coupling Maillard reaction, and preparation method and application of tobacco perfume material Download PDFInfo
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- CN104152268A CN104152268A CN201410382478.5A CN201410382478A CN104152268A CN 104152268 A CN104152268 A CN 104152268A CN 201410382478 A CN201410382478 A CN 201410382478A CN 104152268 A CN104152268 A CN 104152268A
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Abstract
The invention relates to a method for preparing tobacco perfume material prepared by a compound enzyme enzymolysis coupling Maillard reaction. The method comprises the following steps of: 1) grinding waste tobacco shreds, adding water for stirring and cooking, 2) after cooling, treating with compound enzyme, heating up for cooking and enzyme deactivation, 3) adding auxiliary materials such as red date powder, dark plums, spina date seed powder and liquorice for Maillard reaction, and 4) leaching with alcohol at volume percent concentration of 90-100%, filtering, and carrying out vacuum concentration to form an extract, wherein the compound enzyme is cellulase, neutral protease and beta-glucosidase. According to the method, no other chemical reagent is required to be added, and introduction of other adverse smells by additional components is avoided. The method comprehensively utilizes the waste tobacco shreds, changes wastes into valuables, increases an additional value of a product, and reduces wasting of resources and environmental pollution.
Description
Technical field
The present invention relates to Maillard reaction and Application Areas thereof, relate in particular to cigarette perfume material and its preparation method and application prepared by a kind of complex enzyme zymohydrolysis coupling Maillard reaction.
Background technology
China is tobacco big country, and the cultivated area of its tobacco plant and output are all at the forefront in the world, in tobacco redrying, transport, storage and the production of cigarettes course of processing, has a large amount of useless time pipe tobaccos to produce.In inferior tobacco, contain abundant tobacco flavor material and other biologically active substance, therefore, time pipe tobacco that how will give up is turned waste into wealth, and has become a problem that assistant officer is to be solved.To the comprehensive utilization of useless time pipe tobacco, not only can avoid the wasting of resources and the problem of environmental pollution of its generation, can also create more multivalence value simultaneously, become the focus of tobacco industry and the research of association area researcher.
Along with " smoking is with healthy " problem obtains people's extensive concern, tar content in reduction cigarette is imperative, how in reducing tar content, accomplish not weaken fragrance, do not destroy aromatic style, and make cigarette reach good stability to become one of difficult problem of tobacco industry.
Add the enzymolysis that zymin promotes main chemical compositions in tobacco leaf, can make the aroma substance of being combined with biomacromolecule in tobacco discharge more fully, obviously increase cigarette flavor, abirritate, improves smoking property of cigarette quality.Cigarette has the cigarette of enriching perfume (or spice) in cigarette with perfume material, embody the effect of this perfume (or spice) of tobacco.But because Mierocrystalline cellulose in perfume material for the cigarette prepared taking tobacco as raw material, protein equal size are high, make it in cigarette, have increase pungency and the shortcoming such as residual.Cellulase, neutral protease and beta-glucosidase are as lytic enzyme, and its Degradation to Mierocrystalline cellulose, protein, bonding state glycoside compounds etc. in tobacco is comparatively obvious.
Flavoring and casing technology is to promote and improve the flue gas concentration of cigarette and an effective approach of fragrance.The flavor matter reacting by research and utilization Maillard, can give cigarette nature note, makes the fragrant body material of cigarette abundanter, improves the overall quality of cigarette, is that new approach has been opened up in the utilization of the natural resource of China's abundant simultaneously.Still the research report that does not have at present the product of the generation Maillard reactions such as red date, Spina Date Seed, dark plum and Radix Glycyrrhizae to apply in perfuming cigarette.
Summary of the invention
In order to solve above-mentioned technical problem, first object of the present invention is to provide a kind of method that complex enzyme zymohydrolysis coupling Maillard reaction is prepared cigarette perfume material, makes and promotes and improve the flue gas concentration of cigarette and an effective approach of fragrance.Reduce the wasting of resources and environmental pollution simultaneously, improve the added value of tobacco leaf.Second object of the present invention is to provide the application that adopts the cigarette for preparing of above-mentioned method perfume material and this spices.
In order to realize first above-mentioned object, the present invention has adopted following technical scheme:
Complex enzyme zymohydrolysis coupling Maillard reaction is prepared a method for cigarette perfume material, and the method comprises the following steps:
1) will give up that time pipe tobacco is pulverized, the stirring that adds water, 90 ~ 100 DEG C of boiling 20 ~ 40min;
2) be cooled to 30 ~ 45 DEG C, with prozyme processing 1 ~ 3h; Be warming up to 90 ~ 100 DEG C of boiling 20 ~ 40min, enzyme goes out again; Described prozyme adopts cellulase, neutral protease and beta-glucosidase, and the enzymic activity of prozyme is cellulase 100 ~ 300U/g, neutral protease 300 ~ 500U/g, beta-glucosidase 80 ~ 120U/g;
3) add red date powder, dark plum, spiny jujuba seed powder, Radix Glycyrrhizae auxiliary material, carry out Maillard reaction at 100-120 DEG C, time 1 ~ 3h, material rate is useless time pipe tobacco: red date powder: dark plum: spiny jujuba seed powder: Radix Glycyrrhizae=(15 ~ 30): (5 ~ 10): (3 ~ 5): (3 ~ 5): 1;
4) by the alcohol steep of 90% ~ 100% concentration of volume percent, filter, vacuum concentration can obtain medicinal extract.
As preferably, the enzymic activity of described prozyme is cellulase 200U/g, neutral protease 400U/g, beta-glucosidase 100U/g.
As preferably, described useless time pipe tobacco: red date powder: dark plum: spiny jujuba seed powder: Radix Glycyrrhizae=20:(5 ~ 10): (3 ~ 5): (3 ~ 5): 1.
As preferably, doubly, 40 ~ 60 DEG C are extracted cold filtration after 3 ~ 5h to the 10-15 that when described lixiviate, the add-on of ethanol is reacted mixture quality.
As preferably, the water bath heating temperature of described vacuum concentration is 40 ~ 60 DEG C.
In order to realize second above-mentioned object, the present invention has adopted following technical scheme:
The cigarette perfume material that above-mentioned method prepares.This cigarette is with perfume material for tobacco flavoring, and consumption is the 0.005-0.01% of tobacco weight.
A kind of cigarette, the tobacco of this cigarette adopts above-mentioned cigarette perfume material perfuming, and consumption is the 0.005-0.01% of tobacco weight.
The method providing in the present invention does not need additional other chemical reagent, avoids introducing because of additional composition other bad breath.The method makes useless time pipe tobacco obtain comprehensive utilization, turns waste into wealth, and increases added value of product, has reduced the wasting of resources and environmental pollution.The medicinal extract extracting by Maillard reaction is pure natural spice, has great market potential, and its application is easy, easily accomplished in existing Cigarette processing technique, can promote the sucking quality of cigarette.Tobacco extract prepared by the present invention makes tobacco aroma abundanter, has fruit fragrant and sweet, the soft exquisiteness of flue gas, and baking fragrance obviously increases, and significantly improves the suction quality of cigarette etc.
Brief description of the drawings
Fig. 1 is embodiment 1 Maillard reaction perfume material and the affect figure of ethanol extract on cigarette smoke characteristic.
Fig. 2 is embodiment 2 Maillard reaction perfume materials and the affect figure of ethanol extract on cigarette smoke characteristic.
Fig. 3 is embodiment 3 Maillard reaction perfume materials and the affect figure of ethanol extract on cigarette smoke characteristic.
Embodiment
With specific embodiment, technical scheme of the present invention is described below, but protection scope of the present invention is not limited to this.
embodiment 1
To give up time pipe tobacco 1500g, the stirring of pulverizing, add water, 100 DEG C, boiling 30min; Process 2h with 40 DEG C of prozymes, prozyme adopts cellulase, neutral protease and beta-glucosidase, and the enzymic activity of prozyme is cellulase 200U/g, neutral protease 400U/g, beta-glucosidase 100U/g; 100 DEG C of boiling 30min, enzyme goes out; In tobacco, add respectively the auxiliary materials such as red date powder, dark plum, spiny jujuba seed powder, Radix Glycyrrhizae with 20:5:5:5:1, carry out Maillard reaction at 100-120 DEG C; Adding quality is the alcohol steep that its volume fraction of 10 times is 95%, filters, and 50 DEG C of vacuum concentration can obtain medicinal extract, with ten thousand/ ratio join in tobacco.Tobacco is smoked panel test, and note characteristic mainly comprises that this perfume (or spice) of tobacco, delicate fragrance, fragrant and sweet, Xin Xiang, fruital and baking are fragrant etc.; Aroma characteristic mainly comprises fragrance, rich, sophistication, elegant degree and assorted gas size etc., and each index mark 0-9 divides calculating.By professional sensory evaluating smoking, Fig. 1 can find out that the medicinal extract after Maillard reaction is retaining on the basis of this perfume (or spice) of tobacco cigarette product, and fragrance is abundanter, and baking is fragrant to be increased, and Harmony, the consistence of fragrance all make moderate progress.Compare with ethanol extract, the assorted gas of tobacco has obvious reduction simultaneously.
embodiment 2
To give up time pipe tobacco 1500g, the stirring of pulverizing, add water, 100 DEG C, boiling 30min; Process 4h with 40 DEG C of prozymes, prozyme adopts cellulase, neutral protease and beta-glucosidase, and the enzymic activity of prozyme is cellulase 250U/g, neutral protease 350U/g, beta-glucosidase 90U/g; 100 DEG C of boiling 30min, enzyme goes out; In tobacco, add respectively the auxiliary materials such as red date powder, dark plum, spiny jujuba seed powder, Radix Glycyrrhizae with 20:10:5:5:1, carry out Maillard reaction at 100-120 DEG C; Adding quality is the alcohol steep that its volume fraction of 15 times is 95%, filters, and 50 DEG C of vacuum concentration can obtain medicinal extract, join in tobacco with 5/100000ths ratio.Fig. 2 can find out that the medicinal extract after Maillard reaction makes tobacco retaining on the basis of this perfume (or spice) of tobacco, makes fragrance abundanter, and fragrant and sweet and baking is fragrant to be increased, and compares with ethanol extract, and Harmony, the consistence of fragrance all make moderate progress.
embodiment 3
To give up time pipe tobacco 1500g, the stirring of pulverizing, add water, 100 DEG C, boiling 30min; Process 2h with 50 DEG C of prozymes, prozyme adopts cellulase, neutral protease and beta-glucosidase, and the enzymic activity of prozyme is cellulase 150U/g, neutral protease 450U/g, beta-glucosidase 120U/g; 100 DEG C of boiling 30min, enzyme goes out; In tobacco, add respectively the auxiliary materials such as red date powder, dark plum, spiny jujuba seed powder, Radix Glycyrrhizae with 20:5:3:5:1, carry out Maillard reaction at 100-120 DEG C; Adding quality is the alcohol steep that its volume fraction of 10 times is 95%, filters, and 55 DEG C of vacuum concentration can obtain medicinal extract, with ten thousand/ ratio join in tobacco.Fig. 3 can find out that the medicinal extract after Maillard reaction is retaining on the basis of this perfume (or spice) of tobacco tobacco, makes fragrance stronger abundant, and baking is fragrant obviously to be increased, and Harmony, the consistence of fragrance all make moderate progress.Compare with ethanol extract, the assorted gas of tobacco has obvious reduction, and has comparatively strong baking perfume (or spice) simultaneously.
Above-described embodiment is the preferred embodiment of the present invention, but embodiments of the present invention are not restricted to the described embodiments, and the change that other any the present invention of not deviating from does all should be equivalent substitute mode, within being included in protection scope of the present invention.
Claims (8)
1. complex enzyme zymohydrolysis coupling Maillard reaction is prepared a method for cigarette perfume material, it is characterized in that the method comprises the following steps:
1) will give up that time pipe tobacco is pulverized, the stirring that adds water, 90 ~ 100 DEG C of boiling 20 ~ 40min;
2) be cooled to 30 ~ 45 DEG C, with prozyme processing 1 ~ 3h; Be warming up to 90 ~ 100 DEG C of boiling 20 ~ 40min, enzyme goes out again; Described prozyme adopts cellulase, neutral protease and beta-glucosidase, and the enzymic activity of prozyme is cellulase 100 ~ 300U/g, neutral protease 300 ~ 500U/g, beta-glucosidase 80 ~ 120U/g;
3) add red date powder, dark plum, spiny jujuba seed powder, Radix Glycyrrhizae auxiliary material, carry out Maillard reaction at 100-120 DEG C, time 1 ~ 3h, material rate is useless time pipe tobacco: red date powder: dark plum: spiny jujuba seed powder: Radix Glycyrrhizae=(15 ~ 30): (5 ~ 10): (3 ~ 5): (3 ~ 5): 1;
4) by the alcohol steep of 90% ~ 100% concentration of volume percent, filter, vacuum concentration can obtain medicinal extract.
2. a kind of complex enzyme zymohydrolysis coupling Maillard reaction according to claim 1 is prepared the method for cigarette perfume material, it is characterized in that: the enzymic activity of prozyme is cellulase 200U/g neutral protease 400U/g, beta-glucosidase 100U/g.
3. a kind of complex enzyme zymohydrolysis coupling Maillard reaction according to claim 1 is prepared the method for cigarette perfume material, it is characterized in that: useless time pipe tobacco: red date powder: dark plum: spiny jujuba seed powder: Radix Glycyrrhizae=20:(5 ~ 10): (3 ~ 5): (3 ~ 5): 1.
4. the method for preparing cigarette perfume material according to a kind of complex enzyme zymohydrolysis coupling Maillard reaction claimed in claim 1, is characterized in that: the 10-15 that when lixiviate, the add-on of ethanol is reacted mixture quality times, and cold filtration after 40 ~ 60 DEG C of extraction 3 ~ 5h.
5. a kind of complex enzyme zymohydrolysis coupling Maillard reaction according to claim 1 is prepared the method for cigarette perfume material, it is characterized in that: the water bath heating temperature of vacuum concentration is 40 ~ 60 DEG C.
6. the cigarette perfume material preparing according to the method described in claim 1 ~ 5 any one claim.
7. cigarette claimed in claim 6 is with perfume material for tobacco flavoring, and consumption is the 0.005-0.01% of tobacco weight.
8. a cigarette, is characterized in that: the tobacco of this cigarette adopts cigarette claimed in claim 6 perfume material perfuming, and consumption is the 0.005-0.01% of tobacco weight.
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105349266A (en) * | 2015-11-17 | 2016-02-24 | 华宝食用香精香料（上海）有限公司 | Preparation method for grape spice |
| CN106433982A (en) * | 2016-09-21 | 2017-02-22 | 宝可科技（上海）有限公司 | Method for preparing fresh and sweet flavor essence from tobacco leaf stem threads |
| CN107136555A (en) * | 2017-07-05 | 2017-09-08 | 湖北中烟工业有限责任公司 | The method that proline solid phase Maillard reaction improves stem organoleptic quality |
| CN107224003A (en) * | 2017-05-26 | 2017-10-03 | 河南中烟工业有限责任公司 | A kind of enzymolysis alcoholization method for improving reconstituted tobacoo quality |
| CN108703402A (en) * | 2018-06-07 | 2018-10-26 | 湖北中烟工业有限责任公司 | The method that alanine solid phase Maillard reaction improves stem quality |
| CN108707510A (en) * | 2018-06-07 | 2018-10-26 | 湖北中烟工业有限责任公司 | A kind of preparation method and applications of the compound maillard reaction thing of jujube tobacco |
| CN108913357A (en) * | 2018-08-30 | 2018-11-30 | 王婧婧 | A kind of preparation method of red-jujube flavor essence |
| CN112961728A (en) * | 2021-02-04 | 2021-06-15 | 中国烟草总公司海南省公司 | Method for extracting essential oil from cigar tobacco leaves |
| CN114788576A (en) * | 2022-04-07 | 2022-07-26 | 河南中烟工业有限责任公司 | Yuyan extract Maillard reaction spice with outstanding roasted sweet aroma and preparation method and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105349266A (en) * | 2015-11-17 | 2016-02-24 | 华宝食用香精香料（上海）有限公司 | Preparation method for grape spice |
| CN105349266B (en) * | 2015-11-17 | 2019-09-13 | 盐城市春竹香料有限公司 | A kind of preparation method of grape flavor |
| CN106433982A (en) * | 2016-09-21 | 2017-02-22 | 宝可科技（上海）有限公司 | Method for preparing fresh and sweet flavor essence from tobacco leaf stem threads |
| CN107224003A (en) * | 2017-05-26 | 2017-10-03 | 河南中烟工业有限责任公司 | A kind of enzymolysis alcoholization method for improving reconstituted tobacoo quality |
| CN107224003B (en) * | 2017-05-26 | 2018-08-03 | 河南中烟工业有限责任公司 | A kind of enzymolysis -ol method improving reconstituted tobacoo quality |
| CN107136555B (en) * | 2017-07-05 | 2018-06-01 | 湖北中烟工业有限责任公司 | The method that proline solid phase Maillard reaction improves stem organoleptic quality |
| CN107136555A (en) * | 2017-07-05 | 2017-09-08 | 湖北中烟工业有限责任公司 | The method that proline solid phase Maillard reaction improves stem organoleptic quality |
| CN108703402A (en) * | 2018-06-07 | 2018-10-26 | 湖北中烟工业有限责任公司 | The method that alanine solid phase Maillard reaction improves stem quality |
| CN108707510A (en) * | 2018-06-07 | 2018-10-26 | 湖北中烟工业有限责任公司 | A kind of preparation method and applications of the compound maillard reaction thing of jujube tobacco |
| CN108707510B (en) * | 2018-06-07 | 2021-07-06 | 湖北中烟工业有限责任公司 | Preparation method and application of red date and tobacco composite Maillard reactant |
| CN108913357A (en) * | 2018-08-30 | 2018-11-30 | 王婧婧 | A kind of preparation method of red-jujube flavor essence |
| CN112961728A (en) * | 2021-02-04 | 2021-06-15 | 中国烟草总公司海南省公司 | Method for extracting essential oil from cigar tobacco leaves |
| CN114788576A (en) * | 2022-04-07 | 2022-07-26 | 河南中烟工业有限责任公司 | Yuyan extract Maillard reaction spice with outstanding roasted sweet aroma and preparation method and application thereof |
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