CN106433982A - Method for preparing fresh and sweet flavor essence from tobacco leaf stem threads - Google Patents
Method for preparing fresh and sweet flavor essence from tobacco leaf stem threads Download PDFInfo
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- CN106433982A CN106433982A CN201610838241.2A CN201610838241A CN106433982A CN 106433982 A CN106433982 A CN 106433982A CN 201610838241 A CN201610838241 A CN 201610838241A CN 106433982 A CN106433982 A CN 106433982A
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- Prior art keywords
- essence
- cabo
- enzymolysis
- fresh
- powder
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Classifications
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B9/00—Essential oils; Perfumes
- C11B9/02—Recovery or refining of essential oils from raw materials
-
- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
- A24B15/18—Treatment of tobacco products or tobacco substitutes
- A24B15/24—Treatment of tobacco products or tobacco substitutes by extraction; Tobacco extracts
- A24B15/241—Extraction of specific substances
-
- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B3/00—Preparing tobacco in the factory
- A24B3/12—Steaming, curing, or flavouring tobacco
Abstract
The invention provides a method for preparing a fresh and sweet flavor essence from tobacco leaf stem threads. The method comprises the following steps: (1) taking tobacco stem powder, adding water, stirring them uniformly, and then adding fungal amylase and pectase to perform first-stage enzymolysis; (2) after the first-stage enzymolysis reaction, adding protease to perform second-stage enzymolysis; (3) after the second-stage enzymolysis reaction, performing enzyme deactivation on an obtained product, and then cooling the product to obtain tobacco stem enzymatic hydrolysate; and (4) adding glycine and a waxberry extract into the tobacco stem enzymatic hydrolysate, and stirring the materials uniformly to perform Maillard reaction, thereby obtaining the needed essence. The invention further provides application of the essence in tobacco leaves. According to the method for preparing the fresh and sweet flavor essence from the tobacco leaf stem threads, provided by the invention, the preparation is performed by virtue of enzymolysis and Maillard reaction, and the prepared essence has an obvious fresh and sweet flavor, can obviously improve the fragrance quality of cigarettes, enrich cigarette fragrance, thicken fume, and improve the smoking quality of the cigarettes.
Description
Technical field
The invention belongs to cigarette flavoring essence technical field, is related to a kind of method that stems of tobacco leaves prepares fresh and sweet note essence.
Background technology
Nicotiana tabacum L. drafts a document eggplant wood, the annual or limited herbaceos perennial of Solanaceae, base portion slightly lignifying, inflorescence basidixed, circle
Taper, spends more；Capsule ovate or square round shape, length is approximately equal to persistent calyx, and summer and autumn yields positive results, and is distributed mainly on South America, south
Sub-, Chinese.Used as a kind of hobby property consumer products, Nicotiana tabacum L. has substantial amounts of consumer in each place global.But it is near
Nian Lai, with the quickening of globalization of world economy process, " smoking and health " problem is increasingly paid close attention to by society, is therefore dropped
Burnt harm reduction becomes the most important thing of cigarette industry.Generally, the graceful a large amount of damages for inhaling position composition of Medicated cigarette can be caused in terms of reducing tar and reducing harm
Lose, cigarette perfume deficiency, it is difficult to meet consumer demand.In order to solve problem above, many cigar mills are in allotment cigarette composition
While adding substantial amounts of essence, although the deficiency that position inhaled by Medicated cigarette can be so made up, lacking natural sense, having miscellaneous QI to produce.
Cabo refers to stems of tobacco leaves, is the thick and stiff vein of Nicotiana tabacum L., accounts for leaf and weigh 25%～30%.China is tobacco industry
Big country, cabo has few hundred thousand tonnes of to go out of use every year, so both causes environmental pollution as the by-product of tobacco industry maximum,
It is the waste to natural resourcess again.Research shows, the composition species for containing in cabo is basically identical with tobacco leaf ingredient, and cabo has
Great application value realistic.At present, cigarette industry is limited only to fill the products such as expanded cut stem, stalk piece to the utilization of cabo,
But these products can bring much unfavorable aspects to Medicated cigarette, such as cigarette perfume is not enough, flue gas is weak, have stimulation, have miscellaneous QI etc..Cause
This, it is necessary to the application to cabo carries out further research and discussion.
Content of the invention
The shortcoming of prior art in view of the above, it is an object of the invention to provide a kind of stems of tobacco leaves prepares fresh and sweet perfume (or spice)
The method of rhythm essence, adopts with Nicotiana tabacum L. cabo as raw material for solving to lack in prior art, prepares through digesting-Mei Lade method
The problem of the flavouring essence for tobacco with fresh and sweet note.
For achieving the above object and other related purposes, first aspect present invention provides a kind of stems of tobacco leaves and prepares fresh and sweet perfume (or spice)
The method of rhythm essence, comprises the following steps：
1) cabo powder is taken, is added water after stirring, adding fungal amylase and pectase carries out first stage enzymolysis；
Preferably, the cabo is stems of tobacco leaves.The Nicotiana tabacum L. is cured tobacco leaf.
Preferably, the preparation method of the cabo powder, is by stems of tobacco leaves grinding and sieving, obtains final product required cabo powder
End.
It is highly preferred that cabo is ground into powder using pulverizer by described crushing.
It is highly preferred that described sieve used in sieve mesh number >=120 mesh.It is further preferred that described sieve used in sieve
Mesh number be 120 mesh.That is granularity >=120 mesh of the cabo powder, preferably 120 mesh.
Preferably, the water is distilled water.
Preferably, the mass ratio that the cabo powder is added with water is 1：8-12.It is highly preferred that the cabo powder with
The mass ratio that water is added is 1：10.
Preferably, the mass ratio that the cabo powder is added with fungal amylase is 100：0.05-0.15.More preferably
Ground, the mass ratio that the cabo powder is added with fungal amylase is 100：0.1.
Preferably, the fungal amylase is conventional use of fungal amylase.The fungal amylase is Isosorbide-5-Nitrae-α-D-
Glucan hydrolase, also known as fungal alpha-amylase, No. CAS is 9001-19-8.Specifically, the amylomycin used in the present invention
The traditional fungal amylase (Mycolase) that enzyme is produced by DSM (China) company limited (DSM).
Preferably, the mass ratio that the cabo powder is added with pectase is 100：0.05-0.15.It is highly preferred that institute
It is 100 to state the mass ratio that cabo powder added with pectase：0.1.
Preferably, the pectase is conventional use of pectase.No. CAS of the pectase is 9032-75-1.Specifically
Ground, the pectase used in the present invention is the C80 pectase (Rapidase for being produced by DSM (China) company limited (DSM)
C80 Max).
Preferably, the reaction condition of the first stage enzymolysis is：Hydrolysis temperature：50-60℃；Enzymolysis time：1-3h.More
Preferably, the reaction condition of the first stage enzymolysis is：Hydrolysis temperature：55℃；Enzymolysis time：2h.
2) in step 1) described in first stage enzyme digestion reaction terminate after, adding protease carries out second stage enzymolysis；
Preferably, the protease is conventional use of protease.No. CAS of the protease is 9014-01-1.Specifically
Ground, the traditional protease (Maxipro that the protease used in the present invention is produced by DSM (China) company limited (DSM)
NPU).
Preferably, the mass ratio that the cabo powder is added with protease is 100：0.1-0.3.It is highly preferred that described
The mass ratio that cabo powder is added with protease is 100：0.2.
Preferably, the reaction condition of the second stage enzymolysis is：Hydrolysis temperature：50-60℃；Enzymolysis time：1-3h.More
Preferably, the reaction condition of the second stage enzymolysis is：Hydrolysis temperature：55℃；Enzymolysis time：2h.
3) in step 2) described in second stage enzyme digestion reaction terminate after, product is carried out enzyme denaturing, then is cooled down, obtain cabo
Enzyme
Solution liquid；
Preferably, the reaction condition of the enzyme denaturing is：Enzyme-removal temperature：80-90℃；The enzyme denaturing time：10-20min.More preferably
, the reaction condition of the enzyme denaturing is：Enzyme-removal temperature：85℃；The enzyme denaturing time：15min.
Preferably, the cabo enzymolysis solution need not be filtered.
4) by step 3) middle acquisition cabo enzymolysis solution, add Glycine and Fructus Myricae rubrae extract to stir, carry out Mei Lade
Reaction,
Obtain final product required essence.
Preferably, the mass ratio that the cabo powder is added with Glycine is 100：2-4.It is highly preferred that the cabo
The mass ratio that powder is added with Glycine is 100：3.
Preferably, the mass ratio that the cabo powder is added with Fructus Myricae rubrae extract is 10：1-2.It is highly preferred that the cigarette
The mass ratio that stalk powder is added with Fructus Myricae rubrae extract is 10：1.4-1.7.
Preferably, the Fructus Myricae rubrae extract is to extract the poplar for obtaining conventional use of fruit from Fructus Myricae rubrae, rhizome, leaf etc.
Prunus mume extract.Specifically, the Fructus Myricae rubrae extract used in the present invention is produced by Haikou Dao spice company limited.The Fructus Myricae rubrae carries
The mass percent concentration for taking Fructus Myricae rubrae composition in thing accounts for the 80-100% of Fructus Myricae rubrae extract gross mass.
Preferably, the condition of the Maillard reaction is：Reaction temperature：90-110℃；Response time：2-4h.More preferably
, the condition of the Maillard reaction is：Reaction temperature：100℃；Response time：3h.
Preferably, the essence is flavouring essence for tobacco, with fresh and sweet note.
Second aspect present invention provides the fresh and sweet note essence for being prepared by said method.
Third aspect present invention provides a kind of purposes of fresh and sweet note essence in Nicotiana tabacum L..
Preferably, the Nicotiana tabacum L. is cured tobacco leaf.
Fourth aspect present invention provides a kind of using method of fresh and sweet note essence, is to dilute above-mentioned essence with ethanol
Afterwards, essence diluent and cured tobacco leaf are weighed in proportion, essence diluent is uniformly sprayed in cured tobacco leaf.
Preferably, the ethanol is that edible ethanol, the ethanol is the aqueous solution of the dehydrated alcohol containing conventional ratio.More excellent
Selection of land, the ethanol is the edible ethanol containing 70% dehydrated alcohol of percent by volume.
Preferably, the essence diluent is the ethanol solution containing conventional ratio essence.It is highly preferred that the essence dilution
Mass percent in liquid shared by essence is 10%.
Preferably, the essence diluent is conventional mass ratio with flue-cured tobacco group additional proportion.It is highly preferred that described
The mass ratio that essence diluent is added with flue-cured tobacco group is 5：1000.
Preferably, the cured tobacco leaf is the cured tobacco leaf of Yunnan Yuxi's board Medicated cigarette.
Preferably, the sprinkling is carried out using medical spray gun.
As described above, the method that a kind of stems of tobacco leaves that the present invention is provided prepares fresh and sweet note essence, by tobacco stick
Silk enzyme digestion reaction prepares the front extract of reactive essence, carries out Maillard reaction through interpolation Fructus Myricae rubrae extract and obtains with fresh and sweet
The flavouring essence for tobacco of note.Flavouring essence for tobacco prepared by the present invention, with Nicotiana tabacum L. cabo as raw material, abundant raw material source, process is simple, easily
Operation.Flavouring essence for tobacco prepared by the present invention, compared with traditional perfumery essence, with significantly fresh and sweet note, can be obviously improved
The aroma quality of Medicated cigarette, enrich cigarette perfume, enrichment flue gas, promote the production of body fluid sweetened, lifting and be in harmonious proportion the suction quality of Medicated cigarette, effectively can change
Enter the cigarette perfume deficiency of existing general Medicated cigarette, flue gas is weak, have the shortcomings that stimulation, bitter in the mouth, can be widely used in cigarette flavoring essence neck
In domain.
Description of the drawings
Fig. 1 is shown as the GC collection of illustrative plates of the cabo enzymolysis solution that cabo is only obtained in the present invention through enzyme digestion reaction.
Fig. 2 is shown as cabo in the present invention and (joins through the essence for being not added with Fructus Myricae rubrae extract that enzymolysis-Maillard reaction is obtained
Square 11) GC collection of illustrative plates.
Fig. 3 is shown as the essence (formula of the interpolation Fructus Myricae rubrae extract that cabo is obtained in the present invention through enzymolysis-Maillard reaction
15) GC collection of illustrative plates.
Fig. 4 is shown as the GC collection of illustrative plates of stem extracting solution in the present invention.
Fig. 5 is shown as the GC collection of illustrative plates of red big prepared opium paste.
Specific embodiment
The present invention is expanded on further with reference to specific embodiment, it should be appreciated that these embodiments are merely to illustrate the present invention
Rather than limit the scope of the invention.
Below by way of specific instantiation, embodiments of the present invention are described, those skilled in the art can be by this specification
Disclosed content understands other advantages of the present invention and effect easily.The present invention can also pass through in addition different concrete realities
The mode of applying is carried out or applies, and the every details in this specification can also be based on different viewpoints and application, without departing from
Various modifications and changes are carried out under the spirit of the present invention.
It should be clear that in the following example the not concrete process equipment for indicating or device all using conventional equipment in the art or
Device；All pressure value and scope all referring to relative pressure, the present invention using instrument or reagent be routine in the art
Instrument or reagent, all commercially available acquisition.
In addition, it is to be understood that the one or more method and steps that mentions in the present invention do not repel before and after the combination step
Can also there is additive method step or additive method step can also be inserted between the step of these specifically mention, unless separately
It is described；It should also be understood that the combination annexation between the one or more equipment/devices that mentions in the present invention is not repelled
Can also exist before and after the unit equipment/device other equipment/device or two equipment/devices specifically mentioning at these it
Between can also insert other equipment/device, unless otherwise stated.And, unless otherwise stated, the numbering of various method steps is only
Differentiate the convenient tool of various method steps, rather than for limiting the ordering of various method steps or limiting the enforceable model of the present invention
Enclose, being altered or modified for its relativeness, in the case of no essence change technology contents, enforceable when the present invention is also considered as
Category.
Embodiment 1
The sieve of mistake >=120 mesh after stems of tobacco leaves is crushed, cabo powder needed for obtaining.A certain amount of cabo powder is taken, is added
After distilled water stirs, adding fungal amylase and C80 pectase carries out first stage enzymolysis 1-3h at 50-60 DEG C.Its
In, the mass ratio that cabo powder is added with distilled water is 1：8-12；The mass ratio that cabo powder is added with fungal amylase
For 100：0.05-0.15；The mass ratio that cabo powder is added with C80 pectase is 100：0.05-0.15.
Then, adding protease second stage to be carried out at 50-60 DEG C and digesting 1-3h, wherein, cabo powder is added with protease
The mass ratio for entering is 100：0.1-0.3.Enzymatic hydrolysate is carried out enzyme denaturing 10-20min at 80-90 DEG C, then is cooled down, obtain cabo
Enzymolysis solution.Wherein, total enzyme digestion reaction time of first stage enzymolysis and second stage enzymolysis is 2-6h.
Finally by cabo enzymolysis solution, add Glycine and Fructus Myricae rubrae extract to stir, Mei Lade is carried out at 90-110 DEG C
Reaction 2-4h, obtains final product required essence.Wherein, the mass ratio that cabo powder is added with Glycine is 100：2-4；Cabo powder with
The mass ratio that Fructus Myricae rubrae extract is added is 10：1-2.
When above-mentioned essence is used in Nicotiana tabacum L., by above-mentioned essence with the edible ethanol containing 70% dehydrated alcohol of percent by volume
After dilution, the essence diluent that the mass percent shared by essence is 10% is made into, then is added by essence diluent and cured tobacco leaf
The mass ratio for entering is 5：1000 weigh essence diluent and cured tobacco leaf, uniformly spray essence diluent using medical spray gun
It is sprinkled upon in cured tobacco leaf.
Embodiment 2
Carry out digesting in stems of tobacco leaves-during Maillard reaction prepares essence, without Fructus Myricae rubrae extract situation
Under, enzyme digestion reaction temperature is inquired into, impact of the different enzyme digestion reaction temperature to product fragrance is obtained, so as to judge
Optimal enzyme digestion reaction temperature, concrete preparation process is as follows.
The sieve of 120 mesh, cabo powder needed for obtaining is crossed after stems of tobacco leaves is crushed.10.00g cabo powder is taken, is added
After 100.00g distilled water stirs, 0.01g fungal amylase and 0.01g C80 pectase is added in different enzyme digestion reactions
At a temperature of carry out the first stage enzymolysis 2h.
Then, adding 0.02g protease second stage to be carried out at a temperature of 55 DEG C of different enzyme digestion reactions digests 2h, will enzymolysis
Product carries out enzyme denaturing 15min at 85 DEG C, then cools down, and obtains cabo enzymolysis solution.Wherein, first stage enzymolysis and second stage enzymolysis
Total enzyme digestion reaction time be 4h.Enzyme digestion reaction temperature is respectively 50 DEG C, 55 DEG C, 60 DEG C.
Finally by cabo enzymolysis solution, add 0.30g Glycine to stir, Maillard reaction 3h is carried out at 100 DEG C, that is, divide
Not Huo get essence 1#, 2#, 3#, the formula 1,2,3 of essence 1#, 2#, 3#, be shown in Table 1.
As shown in Table 1, in formula 1 enzyme digestion reaction temperature control at 50 DEG C or so, by product is sprayed at Yunnan Yuxi
On the cured tobacco leaf of board Medicated cigarette, find after perfumer's suction, fragrance is not full, has miscellaneous QI, less coordinates with cigarette perfume；Formula 2
Middle enzyme digestion reaction temperature control by suction, is felt fragrant and sweet rhythm still somewhat weakness, but flue gas is dense very thick at 55 DEG C or so,
Fuller than flue gas before；In formula 3, enzyme digestion reaction temperature control is at 60 DEG C or so, and by suction, fragrance is poor, has burnt hardship
Gas, uncoordinated with cigarette perfume.By above 3 recipe ratios relatively, in formula 2, enzyme digestion reaction temperature control, at 55 DEG C, is optimal
Enzyme digestion reaction temperature, hydrolysis temperature at this moment is exactly the maximum activity of enzyme, its overall full aroma, and flue gas is more dense, but burnt
Fragrant and sweet slightly weaker, this may be relevant with the allotment in response time and later stage.
Different impacts of the enzyme digestion reaction temperature to product fragrance of table 1
Embodiment 3
Carry out digesting in stems of tobacco leaves-during Maillard reaction prepares essence, without Fructus Myricae rubrae extract situation
Under, the response time in enzyme digestion reaction is inquired into, impact of the different enzyme digestion reaction times to product fragrance is obtained, from
And judging the optimal enzyme digestion reaction time, concrete preparation process is as follows.
The sieve of 120 mesh, cabo powder needed for obtaining is crossed after stems of tobacco leaves is crushed.10.00g cabo powder is taken, is added
After 100.00g distilled water stirs, adding 0.01g fungal amylase and 0.01g C80 pectase is carried out respectively at 55 DEG C
First stage digests 1h, 2h, 3h.
Then, add 0.02g protease second stage to be carried out respectively at 55 DEG C and digest 1h, 2h, 3h, by enzymatic hydrolysate 85
DEG C enzyme denaturing 15min is carried out, then cool down, obtain cabo enzymolysis solution.Wherein, total enzymolysis of first stage enzymolysis and second stage enzymolysis
Response time is respectively 2h, 4h, 6h.
Finally by cabo enzymolysis solution, add 0.30g Glycine to stir, Maillard reaction 3h is carried out at 100 DEG C, that is, divide
Not Huo get essence 4#, 5#, 6#, the formula 4,5,6 of essence 4#, 5#, 6#, be shown in Table 2.
As shown in Table 2, in formula 4 total enzyme digestion reaction time control in 2h or so, by product is sprayed at Yunnan Yuxi
On the cured tobacco leaf of board Medicated cigarette, find after perfumer's suction, fragrance is not full and uncoordinated with cigarette perfume；Always digest in formula 5
Response time control is in 4h or so, and by suction, fragrant and sweet rhythm still somewhat weakness, but flue gas is dense very thick, than flue gas before
Fuller；In formula 6, total enzyme digestion reaction time control is in 6h or so, and by suction, fragrance is poor, has miscellaneous QI, and rear taste is dry and astringent.Logical
Cross above 3 recipe ratios and relatively understand, in formula 5, total enzyme digestion reaction time control, in 4h, is the optimal enzyme digestion reaction time, now
In the product for going out, fragrant and sweet rhythm is still slightly weak, and this needs adds the raw materials such as a little fruit extracts during Maillard reaction, increases
Fragrant and sweet gas, makes fragrance more coordinate full a bit.
Different impacts of the enzyme digestion reaction time to product fragrance of table 2
Embodiment 4
Carry out digesting in stems of tobacco leaves-during Maillard reaction prepares essence, without Fructus Myricae rubrae extract situation
Under, reaction temperature in Maillard reaction is inquired into, is obtained shadow of the different Maillard reaction temperature to product fragrance
Ring, so as to judge optimal Maillard reaction temperature, concrete preparation process is as follows.
The sieve of 120 mesh, cabo powder needed for obtaining is crossed after stems of tobacco leaves is crushed.10.00g cabo powder is taken, is added
After 100.00g distilled water stirs, adding 0.01g fungal amylase and 0.01g C80 pectase is carried out respectively at 55 DEG C
First stage digests 2h.
Then, adding 0.02g protease second stage to be carried out respectively at 55 DEG C and digesting 2h, enzymatic hydrolysate is carried out at 85 DEG C
Enzyme denaturing 15min, then cool down, obtain cabo enzymolysis solution.Wherein, during total enzyme digestion reaction of first stage enzymolysis and second stage enzymolysis
Between be respectively 4h.
Finally by cabo enzymolysis solution, add 0.30g Glycine to stir, Mei Lade is carried out at a temperature of differential responses anti-
Answer 3h, i.e., respectively obtain essence 7#, 8#, 9#, the formula 7,8,9 of essence 7#, 8#, 9#, be shown in Table 3.Wherein, Maillard reaction temperature
Respectively 90 DEG C, 100 DEG C, 110 DEG C.
As shown in Table 3, in formula 7, Maillard reaction temperature control is at 90 DEG C or so, beautiful by product is sprayed at Yunnan
On the cured tobacco leaf of small stream board Medicated cigarette, find after perfumer's suction, fragrance is not full, has miscellaneous QI, and uncoordinated with cigarette perfume；Formula
In 8, Maillard reaction temperature control is at 100 DEG C or so, by suction, fragrant and sweet rhythm still somewhat weakness, but flue gas is dense very thick,
Fuller than flue gas before；In formula 9, Maillard reaction temperature control is at 110 DEG C or so, and by suction, fragrance is poor, has
Burnt hardship gas.By above 3 recipe ratios relatively, in formula 8 Maillard reaction temperature control at 100 DEG C, Shi Jiamei rad
Reaction temperature.
Different impacts of the Maillard reaction temperature to product fragrance of table 3
Embodiment 5
Carry out digesting in stems of tobacco leaves-during Maillard reaction prepares essence, without Fructus Myricae rubrae extract situation
Under, the response time in Maillard reaction is inquired into, is obtained shadow of the different Maillard reaction times to product fragrance
Ring, so as to judge the optimal Maillard reaction time, concrete preparation process is as follows.
The sieve of 120 mesh, cabo powder needed for obtaining is crossed after stems of tobacco leaves is crushed.10.00g cabo powder is taken, is added
After 100.00g distilled water stirs, adding 0.01g fungal amylase and 0.01g C80 pectase is carried out respectively at 55 DEG C
First stage digests 2h.
Then, adding 0.02g protease second stage to be carried out respectively at 55 DEG C and digesting 2h, enzymatic hydrolysate is carried out at 85 DEG C
Enzyme denaturing 15min, then cool down, obtain cabo enzymolysis solution.Wherein, during total enzyme digestion reaction of first stage enzymolysis and second stage enzymolysis
Between be respectively 4h.
Finally by cabo enzymolysis solution, add 0.30g Glycine to stir, U.S. of different time sections is carried out at 100 DEG C
Maillard reaction, i.e., respectively obtain essence 10#, 11#, 12#, the formula 10,11,12 of essence 10#, 11#, 12#, be shown in Table 4.Wherein,
The Maillard reaction time is respectively 2h, 3h, 4h.
Different impacts of the Maillard reaction time to product fragrance of table 4
As shown in Table 4, in formula 10 Maillard reaction time control in 2h or so, by product is sprayed at Yunnan Yuxi
On the cured tobacco leaf of board Medicated cigarette, find after perfumer's suction, fragrance is not full, uncoordinated with cigarette perfume；11 Sino-U.S.'s rad of formula
Response time control is in 3h or so, and by suction, fragrant and sweet rhythm still somewhat weakness, but flue gas is dense very thick, than flue gas before
Fuller；In formula 12, Maillard reaction time control is in 4h or so, and by suction, fragrance is poor, has obvious burnt hardship gas.Logical
Cross above 3 recipe ratios and relatively understand, in formula 11, Maillard reaction time control, in 3h, is the optimal Maillard reaction time.
Embodiment 6
Carry out digesting in stems of tobacco leaves-during Maillard reaction prepares essence, in order to increase the fresh and sweet note of essence,
Fructus Myricae rubrae extract can be added, therefore, it is necessary to be inquired into the consumption of Fructus Myricae rubrae extract, obtain Fructus Myricae rubrae extract not
With impact of the consumption to product fragrance, so as to judge the consumption of optimal Fructus Myricae rubrae extract, concrete preparation process is as follows.
The sieve of 120 mesh, cabo powder needed for obtaining is crossed after stems of tobacco leaves is crushed.10.00g cabo powder is taken, is added
After 100.00g distilled water stirs, adding 0.01g fungal amylase and 0.01g C80 pectase is carried out respectively at 55 DEG C
First stage digests 2h.
Then, adding 0.02g protease second stage to be carried out respectively at 55 DEG C and digesting 2h, enzymatic hydrolysate is carried out at 85 DEG C
Enzyme denaturing 15min, then cool down, obtain cabo enzymolysis solution.Wherein, during total enzyme digestion reaction of first stage enzymolysis and second stage enzymolysis
Between be respectively 4h.
Finally by cabo enzymolysis solution, 0.30g Glycine and the Fructus Myricae rubrae extract of different content is added to stir, 100
Maillard reaction 3h is carried out at DEG C, i.e., obtain essence 13#, 14#, 15#, 16#, the formula of essence 13#, 14#, 15#, 16# respectively
13rd, 14,15,16, it is shown in Table 5.Wherein, the consumption of Fructus Myricae rubrae extract is respectively 1g, 1.4g, 1.7g, 2g.
Impact of the addition of the different Fructus Myricae rubrae extracts of table 5 to product fragrance
As shown in Table 5, in formula 13, stems of tobacco leaves powder is controlled 10 with the mass ratio of Fructus Myricae rubrae extract consumption:1 is left
The right side, by being sprayed at product on the cured tobacco leaf of Yunnan Yuxi's board Medicated cigarette, finds after perfumer's suction, fragrance is not enough justified
With fragrant and sweet very weak；In formula 14, stems of tobacco leaves powder is controlled 10 with the mass ratio of Fructus Myricae rubrae extract consumption:1.4 or so, lead to
Suction is crossed, flue gas is dense, fragrant and sweet note is higher than formula 13；The quality of stems of tobacco leaves powder and Fructus Myricae rubrae extract consumption in formula 15
Ratio control 10:1.7 or so, by suction, flue gas is dense, and fragrant and sweet note is more preferable, promotes the production of body fluid back sweet；Stems of tobacco leaves in formula 16
Powder is controlled 10 with the mass ratio of Fructus Myricae rubrae extract consumption:2 or so, by suction, fragrance is slightly weak, has obvious caramel
Gas, has miscellaneous QI.By above 4 recipe ratios relatively, stems of tobacco leaves powder is controlled with the mass ratio of Fructus Myricae rubrae extract consumption
10:1.4～10:Between 1.7, optimal Fructus Myricae rubrae extract consumption is obtained in that.
Embodiment 7
In order to examine or check impact of the preparation method to essence quality, respectively by preparing stem extracting solution, only through enzyme digestion reaction
The cabo enzymolysis solution of acquisition, through enzymolysis-Maillard reaction obtain the essence for being not added with Fructus Myricae rubrae extract, through enzymolysis-Mei Lade
The essence of the interpolation Fructus Myricae rubrae extract that reaction is obtained, top-quality red big prepared opium paste, are compared.
First, stem extracting solution is prepared：Will stems of tobacco leaves crush after cross 120 mesh sieve, obtain needed for cabo powder.Take
10.00g cabo powder, after adding 100.00g distilled water to stir, extracts 4h at 70 DEG C, obtains stem extracting solution.
Secondly, the cabo enzymolysis solution for only obtaining through enzyme digestion reaction is prepared：Will stems of tobacco leaves crush after cross 120 mesh sieve,
Cabo powder needed for obtaining.10.00g cabo powder is taken, after adding 100.00g distilled water to stir, adds 0.01g funguses
Amylase and 0.01g C80 pectase carry out first stage enzymolysis 2h respectively at 55 DEG C.0.02g protease is added at 55 DEG C points
Second stage enzymolysis 2h is not carried out, enzymatic hydrolysate is carried out enzyme denaturing 15min at 85 DEG C, then is cooled down and filter, supernatant is taken, obtain
Cabo enzymolysis solution.Wherein, total enzyme digestion reaction time of first stage enzymolysis and second stage enzymolysis is respectively 4h.
Then, the essence for being not added with Fructus Myricae rubrae extract for obtaining through enzymolysis-Maillard reaction is prepared：Its preparation method is with real
Apply the formula 11 in example 5.
The essence of the interpolation Fructus Myricae rubrae extract that through enzymolysis-Maillard reaction obtain is prepared again：Its preparation method is with embodiment 6
In formula 15.
Finally, the red big prepared opium paste for being produced by Haikou Dao spice company limited is directly taken as reactant liquor.
Above-mentioned by acquisition prepares reactant liquor, carries out GC-MS detection respectively, and the testing result of stem extracting solution is shown in Fig. 4, table
6, the testing result of the cabo enzymolysis solution for only obtaining through enzyme digestion reaction is shown in Fig. 1, table 6, through not adding that enzymolysis-Maillard reaction is obtained
Fig. 2, table 6 is seen with the testing result of the essence of Semen arachidis hypogaeae powder, the essence of the interpolation Semen arachidis hypogaeae powder for obtaining through enzymolysis-Maillard reaction
Testing result see Fig. 3, table 6, the testing result of red big prepared opium paste is shown in Fig. 5, table 6.
Wherein, GC-MS testing conditions are：
GC condition：Chromatographic column：INNOWAX, 60m × 0.25mm × 0.25 μm；Carrier gas：He；Flow rate of carrier gas：1mL/min；Enter
Original mold formula：Do not shunt；Injector temperature：250℃；Heating schedule：40 DEG C of holding 6min, rise to 100 DEG C with 3 DEG C/min, then with 5
DEG C/min rise to 230 DEG C holding 10min；
MS condition：Ion source：EI source；Ion source temperature：230℃；Electron energy：70eV；Mass scan range：30～
350amu；Scan pattern：Full scan.
Fragrance component title and its content in 6 differential responses liquid of table
As shown in Table 6, (1) and cabo enzymolysis solution, cabo enzymolysis-Maillard reaction essence, cabo enzymolysis-Maillard reaction
And add Fructus Myricae rubrae essence, red big prepared opium paste and compare, in stem extracting solution, aroma-producing substance species is significantly reduced, and content is relatively low, explanation
The aroma quality of raw material stem is relatively low, is not directly used as Medicated cigarette.(2) raw material stem is after enzymolysis and Maillard reaction, cigarette perfume kind
Class showed increased, and the rising of fragrant, cigarette perfume component content is toasted, green gas, abnormal flavour and low-grade cigarette perfume component content reduce, and illustrate through enzyme
After solution and Maillard reaction, the flavor matter showed increased of raw material stem, in flavouring essence for tobacco, cigarette perfume is not enough, there have to be different to solve which
The shortcomings of taste, miscellaneous QI.(3) Fructus Myricae rubrae extract is added in the Maillard reaction of stem, the content of fresh and sweet perfume composition substantially increases
Plus, baking is fragrant and aroma component content does not only reduce, and slightly raises on the contrary, illustrate the enzymolysis of the stems of tobacco leaves of the present invention-
Maillard reaction liquid has significantly fresh and sweet fragrant, baking note, effectively can be lifted cigarette flavor matter, enrich cigarette perfume, promote the production of body fluid sweetened,
Having improves the effect of cigarette smoking quality.
So, the present invention effectively overcomes various shortcoming of the prior art and has high industrial utilization.
Above-described embodiment only principle of the illustrative present invention and its effect, not for the restriction present invention.Any ripe
The personage for knowing this technology all can carry out modifications and changes without prejudice under the spirit and the scope of the present invention to above-described embodiment.Cause
This, those of ordinary skill in the art is complete with institute under technological thought without departing from disclosed spirit such as
All equivalent modifications for becoming or change, must be covered by the claim of the present invention.
Claims (10)
1. a kind of method that stems of tobacco leaves prepares fresh and sweet note essence, comprises the following steps：
1) cabo powder is taken, is added water after stirring, adding fungal amylase and pectase carries out first stage enzymolysis；
2) in step 1) described in first stage enzyme digestion reaction terminate after, adding protease carries out second stage enzymolysis；
3) in step 2) described in second stage enzyme digestion reaction terminate after, product is carried out enzyme denaturing, then is cooled down, obtain cabo enzymolysis
Liquid；
4) by step 3) middle acquisition cabo enzymolysis solution, add Glycine and Fructus Myricae rubrae extract to stir, Maillard reaction is carried out,
Obtain final product required essence.
2. the method that a kind of stems of tobacco leaves according to claim 1 prepares fresh and sweet note essence, it is characterised in that step 1)
In, the preparation method of the cabo powder, it is by stems of tobacco leaves grinding and sieving, obtains final product required cabo powder.
3. the method that a kind of stems of tobacco leaves according to claim 2 prepares fresh and sweet note essence, it is characterised in that the cigarette
Granularity >=120 mesh of stalk powder.
4. the method that a kind of stems of tobacco leaves according to claim 1 prepares fresh and sweet note essence, it is characterised in that step 1)
In, including any one of following condition or multinomial：
A1) mass ratio that the cabo powder is added with water is 1：8-12；
A2) mass ratio that the cabo powder is added with fungal amylase is 100：0.05-0.15；
A3) mass ratio that the cabo powder is added with pectase is 100：0.05-0.15；
A4) reaction condition of the first stage enzymolysis is：Hydrolysis temperature：50-60℃；Enzymolysis time：1-3h.
5. the method that a kind of stems of tobacco leaves according to claim 1 prepares fresh and sweet note essence, it is characterised in that step 2)
In, including any one of following condition or multinomial：
B1) mass ratio that the cabo powder is added with protease is 100：0.1-0.3；
B2) reaction condition of the second stage enzymolysis is：Hydrolysis temperature：50-60℃；Enzymolysis time：1-3h.
6. the method that a kind of stems of tobacco leaves according to claim 1 prepares fresh and sweet note essence, it is characterised in that step 3)
In, the reaction condition of the enzyme denaturing is：Enzyme-removal temperature：80-90℃；The enzyme denaturing time：10-20min.
7. the method that a kind of stems of tobacco leaves according to claim 1 prepares fresh and sweet note essence, it is characterised in that step 4)
In,
C1) mass ratio that the cabo powder is added with Glycine is 100：2-4；
C2) mass ratio that the cabo powder is added with Fructus Myricae rubrae extract is 10：1-2；
C3) condition of the Maillard reaction is：Reaction temperature：90-110℃；Response time：2-4h.
8. a kind of fresh and sweet note essence, is obtained according to the arbitrary described preparation method of claim 1-7.
9. purposes of a kind of fresh and sweet note essence according to claim 8 in Nicotiana tabacum L..
10. a kind of using method of fresh and sweet note essence according to claim 8, is to dilute above-mentioned essence with ethanol
Afterwards, essence diluent and cured tobacco leaf are weighed in proportion, essence diluent is uniformly sprayed in cured tobacco leaf.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111280475A (en) * | 2020-02-10 | 2020-06-16 | 天津科技大学 | Method for extracting flavor substances in broken tobacco and improving performance of coating by assistance of biological enzyme |
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| JPS6167469A (en) * | 1984-09-11 | 1986-04-07 | 日本たばこ産業株式会社 | Production of fragrance for tobacco |
| CN102247009A (en) * | 2011-07-12 | 2011-11-23 | 广东中烟工业有限责任公司 | Maillard reaction process for tobacco stalk extract |
| CN104152268A (en) * | 2014-08-06 | 2014-11-19 | 浙江中烟工业有限责任公司 | Tobacco perfume material prepared by compound enzyme enzymolysis coupling Maillard reaction, and preparation method and application of tobacco perfume material |
| CN104351944A (en) * | 2014-10-22 | 2015-02-18 | 浙江中烟工业有限责任公司 | Tobacco perfuming agent prepared through molecular distillation treatment of extractum obtained through Maillard reaction from inferior tobacco leaves, preparation method and application of tobacco perfuming agent |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6167469A (en) * | 1984-09-11 | 1986-04-07 | 日本たばこ産業株式会社 | Production of fragrance for tobacco |
| CN102247009A (en) * | 2011-07-12 | 2011-11-23 | 广东中烟工业有限责任公司 | Maillard reaction process for tobacco stalk extract |
| CN104152268A (en) * | 2014-08-06 | 2014-11-19 | 浙江中烟工业有限责任公司 | Tobacco perfume material prepared by compound enzyme enzymolysis coupling Maillard reaction, and preparation method and application of tobacco perfume material |
| CN104351944A (en) * | 2014-10-22 | 2015-02-18 | 浙江中烟工业有限责任公司 | Tobacco perfuming agent prepared through molecular distillation treatment of extractum obtained through Maillard reaction from inferior tobacco leaves, preparation method and application of tobacco perfuming agent |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111280475A (en) * | 2020-02-10 | 2020-06-16 | 天津科技大学 | Method for extracting flavor substances in broken tobacco and improving performance of coating by assistance of biological enzyme |
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