CN106957725A - A kind of preparation method of cigarette Fragaia ananassa Duchesne extract - Google Patents
A kind of preparation method of cigarette Fragaia ananassa Duchesne extract Download PDFInfo
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- CN106957725A CN106957725A CN201610899749.3A CN201610899749A CN106957725A CN 106957725 A CN106957725 A CN 106957725A CN 201610899749 A CN201610899749 A CN 201610899749A CN 106957725 A CN106957725 A CN 106957725A
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- Prior art keywords
- extract
- strawberry
- cigarette
- ananassa duchesne
- fragaia ananassa
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B9/00—Essential oils; Perfumes
- C11B9/02—Recovery or refining of essential oils from raw materials
- C11B9/027—Recovery of volatiles by distillation or stripping
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The present invention provides a kind of preparation method of cigarette Fragaia ananassa Duchesne extract, comprises the following steps：(1) strawberry is put into cold pressing expeller and carries out cold press, taken out juice and obtain strawberry juice, (2), using sodium alginate as carrier, immobilization beta glucuroide is made in embedding, and (3) use immobilization beta glucoside ferment treatment strawberry juice；(4) extract solution after ferment treatment is filtered, strawberry extract solution after filtering is concentrated under reduced pressure, collect concentrate and obtain cigarette Fragaia ananassa Duchesne extract A, Fragaia ananassa Duchesne extract A obtained by step (4) is carried out molecular distillation, takes light component i.e. Fragaia ananassa Duchesne extract by (5).Fragaia ananassa Duchesne extract prepared by the present invention has unique strawberry aroma, and with pleasant fragrant and sweet and fruity note.Fragaia ananassa Duchesne extract is added in cigarette, with abundant cigarette perfume, increases fragrant and sweet and fruity note effect.
Description
Technical field
The present invention relates to the preparation field of essence spice for cigarette, a kind of preparation side of cigarette Fragaia ananassa Duchesne extract is specifically referred to
Method.
Background technology
In tobacco product, the fragrance and suction taste of tobacco are vital, and addition spices is the key in cigarette process
Link, is also to improve one of effective means of product style and raising cigarette flavor quality, some can be provided to consumer fragrant
Gas and the cigarette products for inhaling taste satisfaction.At present, the tobacco aromaticss added in China tobacco are to use conventional methods preparation mostly
, i.e., formed with natural extract monomer and synthon mixing preparation, although so obtained tobacco aromaticss market operation
Simply, but to there is fragrance dull, the shortcomings of improving property to tobacco not enough, and improve cigarette smoking quality DeGrain.
Strawberry (scientific name：Fragaria ananassa Duch, English：Strawberry it is) that rose family Fragaria is perennial
Herbaceous plant, a kind of red flowers and fruits, also known as the red certain kind of berries, the foreign certain kind of berries, the certain kind of berries etc., outward appearance is in heart, and delicious red tender, pulp succulence contains
There is special strong fruit aroma.South America is originated in, all there is cultivation all over the world.Alcohol containing orange perfume, nerolidol, fourth in strawberry
The aroma substances such as sour methyl esters, ethyl butyrate, methyl caproate, ethyl hexanoate, n-octyl butyrate, octyl acetate, caproic acid monooctyl ester, these are fragrant
Gas material has the strawberry notes of feature.And these aromatic substances exist in glycoside link state form mostly, it is difficult to discharge.
If the yield for obtaining aroma substance in spices, spices with customary preparation methods is relatively low so that Fragaia ananassa Duchesne extract is in cigarette
Cigarette perfume is not obvious, so as to limit application of the Fragaia ananassa Duchesne extract in cigarette.
Beta-glucosidase (β-D-Glucosidase), also known as β-D-Glucose glycosides glucose hydrolysis enzyme, alias rough gentian two
Carbohydrase, cellobiase (cellobias) and amygdalase.It belongs to cellulose enzyme, in being cellulose decomposition enzyme system
Important composition composition, can hydrolyze glycoside, while discharging β-D-Glucose and fragrance component.
Immobilised enzymes (immobilized enzyme) is a kind of new technology grown up 1960s, is to use thing
The enzyme of reason or chemical method treatment of water-soluble be allowed to become water insoluble or be fixed on solid phase carrier but still with enzymatic activity
Enzyme derivative.Compared with resolvase, immobilised enzymes can with the long period repeatedly batch reactions and dress post successive reaction, so as to carry
The high service efficiency of enzyme, increases the yield of product, reduces cost；Immobilised enzymes easily divides with product after completion of the reaction
Open, simplify purifying technique, improve the quality of product.Therefore, it is widely used in waste water control, pharmaceutical engineering, chemical industry synthesis etc.
Field, but have no in cigarette is prepared with Fragaia ananassa Duchesne extract report.
The content of the invention
It is an object of the invention to provide a kind of preparation method of cigarette Fragaia ananassa Duchesne extract, the cigarette that it is prepared is carried with strawberry
Take that thing fragrance is more rich, quality is more preferable.
A kind of preparation method of cigarette Fragaia ananassa Duchesne extract, comprises the following steps：
(1) strawberry is cleaned, removes epidermis, be put into cold pressing expeller and carry out cold press, take out juice and obtain strawberry juice；
(2) 0.1~0.3g solid beta-glucosidases are dissolved in 1mL citrate buffer solutions (0.1mol/L, pH4.5),
The sodium alginate soln that 15~20mL mass concentrations are 2% is added, is stirred, it is 2% to add 20~30mL mass concentrations
Calcium chloride solution, 4 DEG C embedding 1~3h after, washed with cushioning liquid, obtain immobilized β-glucosidase, its bearing capacity be 10
~20U/g；
(3) take immobilized β-glucosidase handle strawberry juice, immobilized β-glucosidase solid consumption 10g/L~
40g/L, the pH value 6~8 of ferment treatment, 35 DEG C~45 DEG C of ferment treatment temperature, enzyme processing time 3h~5h, at 2 DEG C after the completion of processing
~5 DEG C of settlement treatment 24h~48h；
(4) extract solution after ferment treatment is filtered, obtains the strawberry extract solution of clarification, the strawberry extract solution after filtering is depressurized
Concentration, it is 1.30~1.35 to be concentrated into relative density, collects concentrate and obtains Fragaia ananassa Duchesne extract A；
(5) the Fragaia ananassa Duchesne extract A of step (4) is taken, propane diols is added, in 6~20Pa of pressure, feeding temperature is 60 DEG C~75
DEG C, charging rate is 5~30mL/min, and scraper plate rotating speed is to carry out molecular distillation under conditions of 60~130rpm, takes the light component to be
Fragaia ananassa Duchesne extract；The percentage that the addition of wherein propane diols accounts for molecular distillation thing gross mass is 10%~30%.
Further, when the extract solution in the step (4) after ferment treatment is filtered, crossed with filter paper and filter off the miscellaneous of bulky grain
Matter, obtains the strawberry extract solution of clarification.
Further, when the step (4) is concentrated under reduced pressure, thickening temperature is 50~75 DEG C, and concentration pressure is 0.01~0.1
Individual atmospheric pressure.
Beneficial effects of the present invention：The present invention is handled in Fragaia ananassa Duchesne extract, hydrolysis strawberry with immobilized β-glucosidase
Aroma precursor material, discharge potential fragrance ingredient, greatly increase the extraction yield of the aromatic substance in strawberry, reduction life
Cost is produced, obtains that fragrance is more rich, the more preferable characteristic cigarette flavor of quality.Fragaia ananassa Duchesne extract prepared by the present invention has only
Special strawberry aroma, and with pleasant fragrant and sweet and fruity note.Fragaia ananassa Duchesne extract is added in cigarette, with abundant cigarette
Fragrant, fragrant and sweet and fruity note the effect of increase.Present invention improves the quality of Fragaia ananassa Duchesne extract, Fragaia ananassa Duchesne extract is realized in volume
Application in cigarette.And this method can be easy to operate and automated by instrument precision control, and cost is more cheap, easy
Production conversion.
Strawberry juice, release orange perfume alcohol, nerolidol, methyl butyrate, butyric acid second are handled using immobilized β-glucosidase
Aromatic substance in the aromatic substances such as ester, methyl caproate, ethyl hexanoate, n-octyl butyrate, octyl acetate, caproic acid monooctyl ester, increase strawberry
The extraction yield of matter, so as to reduce production cost.The cigarette Fragaia ananassa Duchesne extract of characteristic is prepared, this method technique is simple, low cost,
Extract yield high, fragrance-quality is good.Compared with free beta-glucosidase ferment treatment strawberry juice, after immobilization processing, it is not necessary to logical
Membrane separation removes resolvase, simplifies production technology, is easy to industrialized production to convert；Key odorant material in Fragaia ananassa Duchesne extract
Key odorant material orange perfume alcohol, nerolidol, methyl butyrate, ethyl butyrate, methyl caproate, ethyl hexanoate, n-octyl butyrate, acetic acid
The total relative amount of monooctyl ester, caproic acid monooctyl ester improves 39.25%, is added in cigarette, and cigarette flavor more enriches, fragrant and sweet
Rhythm is more obvious.
Embodiment
The relevant technical problem of the present invention is described in further detail with reference to embodiment.Embodiment 1
(1) strawberry is cleaned, removes epidermis, be put into cold pressing expeller and carry out cold press, take out juice and obtain strawberry juice；
(2) 0.2g solid beta-glucosidases are dissolved in 1mL citrate buffer solutions (0.1mol/L, pH4.5), added
18mL mass concentrations are 2% sodium alginate soln, are stirred, and add the calcium chloride solution that 26mL mass concentrations are 2%,
After 4 DEG C of embedding 1.5h, washed with cushioning liquid, obtain Immobilization in Sodium Alginate enzyme, its bearing capacity is 15U/g；
(3) immobilized β-glucosidase is taken to handle strawberry juice, immobilized β-glucosidase solid consumption 15g/L, enzyme
The pH value 6 of processing, 35 DEG C of ferment treatment temperature, enzyme processing time 3.5h, in 2 DEG C of settlement treatment 36h after the completion of processing；
(4) extract solution after ferment treatment is crossed to the impurity for filtering off bulky grain with filter paper, the strawberry extract solution of clarification is obtained, incited somebody to action
Strawberry extract solution after filter is concentrated under reduced pressure, and thickening temperature is 55 DEG C, and concentration pressure is concentrated into relative density for 0.03 atmospheric pressure
For 1.31, collect concentrate and obtain cigarette Fragaia ananassa Duchesne extract A；
(5) the Fragaia ananassa Duchesne extract A of step (4) is taken, propane diols is added, in pressure 7Pa, feeding temperature is 62 DEG C, charging speed
Spend for 8mL/min, scraper plate rotating speed is to carry out molecular distillation under conditions of 70rpm, and it is Fragaia ananassa Duchesne extract to take light component；Wherein the third two
The percentage that the addition of alcohol accounts for molecular distillation thing gross mass is 12%.
Embodiment 2
(1) strawberry is cleaned, removes epidermis, be put into cold pressing expeller and carry out cold press, take out juice and obtain strawberry juice；
(2) 0.15g solid beta-glucosidases are dissolved in 1mL citrate buffer solutions (0.1mol/L, pH4.5), added
15mL mass concentrations are 2% sodium alginate soln, are stirred, and add the calcium chloride solution that 20mL mass concentrations are 2%,
After 4 DEG C of embedding 2h, washed with cushioning liquid, obtain immobilized β-glucosidase, its bearing capacity is 10U/g.
(3) immobilized β-glucosidase is taken to handle strawberry juice, immobilized β-glucosidase solid consumption 26g/L, enzyme
The pH value 7 of processing, 38 DEG C of ferment treatment temperature, enzyme processing time 4.2h, in 3 DEG C of settlement treatment 40h after the completion of processing；
(4) extract solution after ferment treatment is crossed to the impurity for filtering off bulky grain with filter paper, the strawberry extract solution of clarification is obtained, incited somebody to action
Strawberry extract solution after filter is concentrated under reduced pressure, and thickening temperature is 60 DEG C, and concentration pressure is concentrated into relative density for 0.10 atmospheric pressure
For 1.32, collect concentrate and obtain cigarette Fragaia ananassa Duchesne extract A；
(5) the Fragaia ananassa Duchesne extract A of step (4) is taken, propane diols is added, in pressure 12Pa, feeding temperature is 68 DEG C, charging speed
Spend for 18mL/min, scraper plate rotating speed is to carry out molecular distillation under conditions of 85rpm, and it is Fragaia ananassa Duchesne extract to take light component；Wherein third
The percentage that the addition of glycol accounts for molecular distillation thing gross mass is 21%.
Embodiment 3
(1) strawberry is cleaned, removes epidermis, be put into cold pressing expeller and carry out cold press, take out juice and obtain strawberry juice；
(2) 0.3g solid beta-glucosidases are dissolved in 1mL citrate buffer solutions (0.1mol/L, pH4.5), added
20mL mass concentrations are 2% sodium alginate soln, are stirred, and add the calcium chloride solution that 30mL mass concentrations are 2%,
After 4 DEG C of embedding 3h, washed with cushioning liquid, obtain immobilized β-glucosidase, its bearing capacity is 20U/g.
(3) immobilized β-glucosidase is taken to handle strawberry juice, immobilized β-glucosidase solid consumption 38g/L, enzyme
The pH value 8 of processing, 43 DEG C of ferment treatment temperature, enzyme processing time 4.8h, in 5 DEG C of settlement treatment 48h after the completion of processing；
(4) extract solution after ferment treatment is crossed to the impurity for filtering off bulky grain with filter paper, the strawberry extract solution of clarification is obtained, incited somebody to action
Strawberry extract solution after filter is concentrated under reduced pressure, and thickening temperature is 75 DEG C, and concentration pressure is concentrated into relative density for 0.08 atmospheric pressure
For 1.35, collect concentrate and obtain cigarette Fragaia ananassa Duchesne extract A；
(5) the Fragaia ananassa Duchesne extract A of step (4) is taken, propane diols is added, in pressure 19Pa, feeding temperature is 73 DEG C, charging speed
Spend for 27mL/min, scraper plate rotating speed is to carry out molecular distillation under conditions of 125rpm, and it is Fragaia ananassa Duchesne extract to take light component；Wherein third
The percentage that the addition of glycol accounts for molecular distillation thing gross mass is 27%.
The present invention handles strawberry juice using immobilized β-glucosidase, and it is identical with the present invention with other steps, and
Processing mode using free beta-glucosidase ferment treatment strawberry extract solution compares, as shown in table 1, is Different treatments strawberry
Orange perfume alcohol, nerolidol, methyl butyrate, ethyl butyrate, methyl caproate, ethyl hexanoate, n-octyl butyrate, acetic acid are pungent in extract
The total relative amount of ester, caproic acid monooctyl ester compares, and as shown in table 2, by the Fragaia ananassa Duchesne extract under Different treatments, is added to cigarette
In cigarette smoking result.
The aroma substance relative amount of table 1 compares
Sensory evaluation effect of the Fragaia ananassa Duchesne extract of the Different treatments of table 2 in cigarette
The preparation method of the present invention makes key odorant material orange perfume alcohol, nerolidol, methyl butyrate, fourth in Fragaia ananassa Duchesne extract
The total relative amount of acetoacetic ester, methyl caproate, ethyl hexanoate, n-octyl butyrate, octyl acetate, caproic acid monooctyl ester improves 39.25%, will
It is added in cigarette, and cigarette flavor more enriches, and fragrant and sweet rhythm is more obvious.The extracting method using the present invention is absolutely proved
Prepare that yield is higher, the more preferable Fragaia ananassa Duchesne extract of quality.
The detailed description of specific embodiments of the present invention is the foregoing is only, the present invention is not limited with this, it is all at this
Any modification, equivalent and improvement for being made in the mentality of designing of invention etc., should be included in protection scope of the present invention it
It is interior.
Claims (3)
1. a kind of preparation method of cigarette Fragaia ananassa Duchesne extract, it is characterised in that comprise the following steps：
(1) strawberry is cleaned, removes epidermis, be put into cold pressing expeller and carry out cold press, take out juice and obtain strawberry juice；
(2) 0.1~0.3g solid beta-glucosidases are dissolved in 1mL citrate buffer solutions (0.1mol/L, pH4.5), added
15~20mL mass concentrations are 2% sodium alginate soln, are stirred, and add the chlorine that 20~30mL mass concentrations are 2%
Change calcium solution, 4 DEG C embedding 1~3h after, washed with cushioning liquid, obtain immobilized β-glucosidase, its bearing capacity be 10~
20U/g；
(3) take immobilized β-glucosidase handle strawberry juice, immobilized β-glucosidase solid consumption 10g/L~40g/L,
The pH value 6~8 of ferment treatment, 35 DEG C~45 DEG C of ferment treatment temperature, enzyme processing time 3h~5h is heavy at 2 DEG C~5 DEG C after the completion of processing
Drop processing 24h~48h；
(4) extract solution after ferment treatment is filtered, obtains the strawberry extract solution of clarification, the strawberry extract solution decompression after filtering is dense
Contracting, it is 1.30~1.35 to be concentrated into relative density, collects concentrate and obtains Fragaia ananassa Duchesne extract A；
(5) the Fragaia ananassa Duchesne extract A of step (4) is taken, propane diols is added, in 6~20Pa of pressure, feeding temperature is 60 DEG C~75 DEG C,
Charging rate is 5~30mL/min, and scraper plate rotating speed is carries out molecular distillation under conditions of 60~130rpm, and it is strawberry to take light component
Extract；The percentage that the addition of wherein propane diols accounts for molecular distillation thing gross mass is 10%~30%.
2. the preparation method of the cigarette Fragaia ananassa Duchesne extract described in claim 1 according to claim 1, it is characterised in that：
When extract solution in the step (4) after ferment treatment is filtered, the impurity for filtering off bulky grain is crossed with filter paper, the strawberry for obtaining clarification is extracted
Liquid.
3. the preparation method of cigarette Fragaia ananassa Duchesne extract according to claim 1, it is characterised in that：Step (4) decompression
During concentration, thickening temperature is 50~75 DEG C, and concentration pressure is 0.01~0.1 atmospheric pressure.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610899749.3A CN106957725A (en) | 2016-10-13 | 2016-10-13 | A kind of preparation method of cigarette Fragaia ananassa Duchesne extract |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610899749.3A CN106957725A (en) | 2016-10-13 | 2016-10-13 | A kind of preparation method of cigarette Fragaia ananassa Duchesne extract |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111066887A (en) * | 2019-12-30 | 2020-04-28 | 光明乳业股份有限公司 | Essence-free fermented milk beverage and preparation method thereof |
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| CN102150948A (en) * | 2011-05-19 | 2011-08-17 | 湖北中烟工业有限责任公司 | Method for preparing jasmine extract for tobacco |
| CN105754722A (en) * | 2016-03-14 | 2016-07-13 | 湖北中烟工业有限责任公司 | Preparation method and application of tobacco-aroma matters for tobacco sheets |
-
2016
- 2016-10-13 CN CN201610899749.3A patent/CN106957725A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102150948A (en) * | 2011-05-19 | 2011-08-17 | 湖北中烟工业有限责任公司 | Method for preparing jasmine extract for tobacco |
| CN105754722A (en) * | 2016-03-14 | 2016-07-13 | 湖北中烟工业有限责任公司 | Preparation method and application of tobacco-aroma matters for tobacco sheets |
Non-Patent Citations (1)
| Title |
|---|
| 范刚: "《柑橘及其加工制品中游离态和键合态挥发性物质的研究》", 15 May 2010, 中国优秀博士学位论文全文数据库工程科技1辑 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111066887A (en) * | 2019-12-30 | 2020-04-28 | 光明乳业股份有限公司 | Essence-free fermented milk beverage and preparation method thereof |
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