CN106957721A - A kind of cigarette preparation method of peach extract - Google Patents
A kind of cigarette preparation method of peach extract Download PDFInfo
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- CN106957721A CN106957721A CN201610895917.1A CN201610895917A CN106957721A CN 106957721 A CN106957721 A CN 106957721A CN 201610895917 A CN201610895917 A CN 201610895917A CN 106957721 A CN106957721 A CN 106957721A
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- Prior art keywords
- peach
- cigarette
- extract
- peach extract
- juice
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B9/00—Essential oils; Perfumes
- C11B9/02—Recovery or refining of essential oils from raw materials
-
- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
- A24B15/18—Treatment of tobacco products or tobacco substitutes
- A24B15/24—Treatment of tobacco products or tobacco substitutes by extraction; Tobacco extracts
- A24B15/241—Extraction of specific substances
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/10—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a carbohydrate
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/14—Enzymes or microbial cells immobilised on or in an inorganic carrier
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
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- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
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- Engineering & Computer Science (AREA)
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- Bioinformatics & Cheminformatics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Microbiology (AREA)
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- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- General Chemical & Material Sciences (AREA)
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- Inorganic Chemistry (AREA)
- Molecular Biology (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Fats And Perfumes (AREA)
Abstract
The present invention provides a kind of cigarette preparation method of peach extract, comprises the following steps：(1) peach is put into cold pressing expeller and carries out cold press, taken out juice and obtain peach juice, (2), using sodium alginate as carrier, immobilization beta glucuroide is made in embedding, and (3) use immobilization beta glucoside ferment treatment peach juice；(4) extract solution after ferment treatment is filtered, the peach extract solution after filtering is concentrated under reduced pressure, collected concentrate and obtain cigarette peach extract.Peach extract prepared by the present invention has unique peach fragrance, and with pleasant fragrant and sweet and fruity note.Peach extract is added in cigarette, with abundant cigarette perfume, increases fragrant and sweet and fruity note effect.
Description
Technical field
The present invention relates to the preparation field of essence spice for cigarette, a kind of preparation side of cigarette peach extract is specifically referred to
Method.
Background technology
In tobacco product, the fragrance and suction taste of tobacco are vital, and addition spices is the key in cigarette process
Link, is also to improve one of effective means of product style and raising cigarette flavor quality, some can be provided to consumer fragrant
Gas and the cigarette products for inhaling taste satisfaction.At present, the tobacco aromaticss added in China tobacco are to use conventional methods preparation mostly
, i.e., formed with natural extract monomer and synthon mixing preparation, although so obtained tobacco aromaticss market operation
Simply, but to there is fragrance dull, the shortcomings of improving property to tobacco not enough, and improve cigarette smoking quality DeGrain.
Peach (scientific name：Amygdalus persica L.) it is that rose family peach belongs to perennial dungarunga, its flower section views and admires, fruit
Real succulence, can eat raw or preserved peach processed, can etc., peach kernel also edible.In peach containing γ-hexalactone, cis -2- hexenols,
The aroma substances such as γ-decalactone, leaf-alcohol, hexanol, these aroma substances have the peach note of feature.And these aromatic substances are big
It is many to exist in glycoside link state form, it is difficult to discharge.If obtaining aroma substance in spices, spices with customary preparation methods
Yield it is relatively low so that cigarette perfume of the peach extract in cigarette be not obvious, so as to limit peach extract in cigarette
Using.
Beta-glucosidase (β-D-Glucosidase), also known as β-D-Glucose glycosides glucose hydrolysis enzyme, alias rough gentian two
Carbohydrase, cellobiase (cellobias) and amygdalase.It belongs to cellulose enzyme, in being cellulose decomposition enzyme system
Important composition composition, can hydrolyze glycoside, while discharging β-D-Glucose and fragrance component.
Immobilised enzymes (immobilized enzyme) is a kind of new technology grown up 1960s, is to use thing
The enzyme of reason or chemical method treatment of water-soluble be allowed to become water insoluble or be fixed on solid phase carrier but still with enzymatic activity
Enzyme derivative.Compared with resolvase, immobilised enzymes can with the long period repeatedly batch reactions and dress post successive reaction, so as to carry
The high service efficiency of enzyme, increases the yield of product, reduces cost；Immobilised enzymes easily divides with product after completion of the reaction
Open, simplify purifying technique, improve the quality of product.Therefore, it is widely used in waste water control, pharmaceutical engineering, chemical industry synthesis etc.
Field, but have no report in cigarette is prepared with peach extract.
The content of the invention
It is an object of the invention to provide a kind of preparation method of cigarette with peach extract, the cigarette that it is prepared is carried with peach
Take that thing fragrance is more rich, quality is more preferable.
A kind of cigarette preparation method of peach extract, comprises the following steps：
(1) peach is cleaned, removes epidermis, be put into cold pressing expeller and carry out cold press, taken out juice and obtain peach juice；
(2) 0.1~0.3g solid beta-glucosidases are dissolved in 1mL citrate buffer solutions (0.1mol/L, pH4.5),
The sodium alginate soln that 15~20mL mass concentrations are 2% is added, is stirred, it is 2% to add 20~30mL mass concentrations
Calcium chloride solution, 4 DEG C embedding 1~3h after, washed with cushioning liquid, obtain immobilized β-glucosidase, its bearing capacity be 10
~20U/g；
(3) take immobilized β-glucosidase handle peach juice, immobilized β-glucosidase solid 5~8g/L of consumption,
The pH value 5~8 of ferment treatment, 35~45 DEG C of ferment treatment temperature, 5~10h of enzyme processing time, after the completion of processing at 3~6 DEG C of sedimentations
Manage 24~36h；
(4) extract solution after ferment treatment is filtered, obtains the peach extract solution of clarification, the peach extract solution after filtering is depressurized
Concentration, it is 1.10~1.35 to be concentrated into relative density, collects concentrate and obtains cigarette peach extract.
Further, when the extract solution in the step (4) after ferment treatment is filtered, crossed with filter paper and filter off the miscellaneous of bulky grain
Matter, obtains the peach extract solution of clarification.
Further, when the step (4) is concentrated under reduced pressure, thickening temperature is 50~75 DEG C, and concentration pressure is 0.01~0.1
Individual atmospheric pressure.
Beneficial effects of the present invention：The present invention is handled in peach extract, hydrolysis peach with immobilized β-glucosidase
Aroma precursor material, discharge potential fragrance ingredient, greatly increase the extraction yield of the aromatic substance in peach, reduction life
Cost is produced, obtains that fragrance is more rich, the more preferable characteristic cigarette flavor of quality.Peach extract prepared by the present invention has only
Special peach fragrance, and with pleasant fragrant and sweet and fruity note.Peach extract is added in cigarette, with abundant cigarette
Fragrant, fragrant and sweet and fruity note the effect of increase.Present invention improves the quality of peach extract, peach extract is realized in volume
Application in cigarette.And this method can be easy to operate and automated by instrument precision control, and cost is more cheap, easy
Production conversion.
Peach juice is handled using immobilized β-glucosidase, γ-hexalactone, cis -2- hexenols is discharged, in γ-last of the ten Heavenly stems
The extraction yield of aromatic substance in ester, leaf-alcohol, hexanol etc., increase peach, so as to reduce production cost.The cigarette for preparing characteristic is used
Peach extract, this method technique is simple, low cost, extracts yield height, and fragrance-quality is good.With free beta-glucosidase ferment treatment
Peach juice is compared, and after immobilization processing, it is not necessary to remove resolvase by UF membrane, simplify production technology, is easy to industrialization
Production conversion；The loss extracted in separation process is reduced, yield improves 5.5%~8.1%；It is main fragrant in peach extract
The total relative amount of gas material γ-hexalactone, cis -2- hexenols, γ-decalactone, leaf-alcohol, hexanol improves 23%~
48%, it is added in cigarette, cigarette flavor more enriches, and fragrant and sweet rhythm is more obvious.
Embodiment
The relevant technical problem of the present invention is described in further detail with reference to embodiment.Embodiment 1
(1) peach is cleaned, removes epidermis, be put into cold pressing expeller and carry out cold press, taken out juice and obtain peach juice；
(2) 0.2g solid beta-glucosidases are dissolved in 1mL citrate buffer solutions (0.1mol/L, pH4.5), added
18mL mass concentrations are 2% sodium alginate soln, are stirred, and add the calcium chloride solution that 26mL mass concentrations are 2%,
After 4 DEG C of embedding 1.5h, washed with cushioning liquid, obtain Immobilization in Sodium Alginate enzyme, its bearing capacity is 15U/g；
(3) immobilized β-glucosidase is taken to handle at peach juice, immobilized β-glucosidase solid consumption 6g/L, enzyme
The pH value 5 of reason, 37 DEG C of ferment treatment temperature, enzyme processing time 8h, in 3 DEG C of settlement treatment 26h after the completion of processing；
(4) extract solution after ferment treatment is crossed to the impurity for filtering off bulky grain with filter paper, the peach extract solution of clarification is obtained, incited somebody to action
Peach extract solution after filter is concentrated under reduced pressure, and thickening temperature is 55 DEG C, and concentration pressure is concentrated into relative density for 0.03 atmospheric pressure
For 1.16, collect concentrate and obtain cigarette peach extract.
Embodiment 2
(1) peach is cleaned, removes epidermis, be put into cold pressing expeller and carry out cold press, taken out juice and obtain peach juice；
(2) 0.15g solid beta-glucosidases are dissolved in 1mL citrate buffer solutions (0.1mol/L, pH4.5), added
15mL mass concentrations are 2% sodium alginate soln, are stirred, and add the calcium chloride solution that 20mL mass concentrations are 2%,
After 4 DEG C of embedding 2h, washed with cushioning liquid, obtain immobilized β-glucosidase, its bearing capacity is 10U/g.
(3) immobilized β-glucosidase is taken to handle at peach juice, immobilized β-glucosidase solid consumption 7g/L, enzyme
The pH value 7 of reason, 40 DEG C of ferment treatment temperature, enzyme processing time 6h, in 5 DEG C of settlement treatment 30h after the completion of processing；
(4) extract solution after ferment treatment is crossed to the impurity for filtering off bulky grain with filter paper, the peach extract solution of clarification is obtained, incited somebody to action
Peach extract solution after filter is concentrated under reduced pressure, and thickening temperature is 60 DEG C, and concentration pressure is concentrated into relative density for 0.10 atmospheric pressure
For 1.20, collect concentrate and obtain cigarette peach extract.
Embodiment 3
(1) peach is cleaned, removes epidermis, be put into cold pressing expeller and carry out cold press, taken out juice and obtain peach juice；
(2) 0.3g solid beta-glucosidases are dissolved in 1mL citrate buffer solutions (0.1mol/L, pH4.5), added
20mL mass concentrations are 2% sodium alginate soln, are stirred, and add the calcium chloride solution that 30mL mass concentrations are 2%,
After 4 DEG C of embedding 3h, washed with cushioning liquid, obtain immobilized β-glucosidase, its bearing capacity is 20U/g.
(3) immobilized β-glucosidase is taken to handle at peach juice, immobilized β-glucosidase solid consumption 8g/L, enzyme
The pH value 8 of reason, 44 DEG C of ferment treatment temperature, enzyme processing time 10h, in 6 DEG C of settlement treatment 35h after the completion of processing；
(4) extract solution after ferment treatment is crossed to the impurity for filtering off bulky grain with filter paper, the peach extract solution of clarification is obtained, incited somebody to action
Peach extract solution after filter is concentrated under reduced pressure, and thickening temperature is 75 DEG C, and concentration pressure is concentrated into relative density for 0.08 atmospheric pressure
For 1.32, collect concentrate and obtain cigarette peach extract.
The present invention handles peach juice using immobilized β-glucosidase, and it is identical with the present invention with other steps, and
Processing mode using free beta-glucosidase ferment treatment peach extract solution compares, as shown in table 1, is Different treatments peach
The total relative amount ratio of key odorant material γ-hexalactone, cis -2- hexenols, γ-decalactone, leaf-alcohol, hexanol in extract
Compared with as shown in table 2, by the peach extract under Different treatments, being added to the cigarette smoking result in cigarette.
The key odorant material relative amount of table 1 compares
Sensory evaluation effect of the peach extract of the Different treatments of table 2 in cigarette
The preparation method of the present invention makes the yield of peach extract improve 5.5%~8.1%；Above experimental result table
It is bright：The total phase of key odorant material γ-hexalactone, cis -2- hexenols, γ-decalactone, leaf-alcohol, hexanol in peach extract
30.53% is improved to content, is added in cigarette, cigarette flavor more enriches, and fragrant and sweet rhythm is more obvious.Absolutely prove
Prepare that yield is higher, quality more preferable peach extract using the extracting method of the present invention.
The detailed description of specific embodiments of the present invention is the foregoing is only, the present invention is not limited with this, it is all at this
Any modification, equivalent and improvement for being made in the mentality of designing of invention etc., should be included in protection scope of the present invention it
It is interior.
Claims (3)
1. a kind of cigarette preparation method of peach extract, it is characterised in that comprise the following steps：
(1) peach is cleaned, removes epidermis, be put into cold pressing expeller and carry out cold press, taken out juice and obtain peach juice；
(2) 0.1~0.3g solid beta-glucosidases are dissolved in 1mL citrate buffer solutions (0.1mol/L, pH4.5), added
15~20mL mass concentrations are 2% sodium alginate soln, are stirred, and add the chlorine that 20~30mL mass concentrations are 2%
Change calcium solution, 4 DEG C embedding 1~3h after, washed with cushioning liquid, obtain immobilized β-glucosidase, its bearing capacity be 10~
20U/g；
(3) immobilized β-glucosidase is taken to handle at peach juice, immobilized β-glucosidase solid 5~8g/L of consumption, enzyme
The pH value 5~8 of reason, 35~45 DEG C of ferment treatment temperature, 5~10h of enzyme processing time, in 3~6 DEG C of settlement treatments 24 after the completion of processing
~36h；
(4) extract solution after ferment treatment is filtered, obtains the peach extract solution of clarification, the peach extract solution decompression after filtering is dense
Contracting, it is 1.10~1.35 to be concentrated into relative density, collects concentrate and obtains cigarette peach extract.
2. the cigarette according to claim 1 preparation method of peach extract, it is characterised in that：Enzyme in the step (4)
When extract solution after processing is filtered, the impurity for filtering off bulky grain is crossed with filter paper, the peach extract solution of clarification is obtained.
3. the cigarette according to claim 1 preparation method of peach extract, it is characterised in that：Step (4) decompression
During concentration, thickening temperature is 50~75 DEG C, and concentration pressure is 0.01~0.1 atmospheric pressure.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610895917.1A CN106957721A (en) | 2016-10-13 | 2016-10-13 | A kind of cigarette preparation method of peach extract |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610895917.1A CN106957721A (en) | 2016-10-13 | 2016-10-13 | A kind of cigarette preparation method of peach extract |
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| Publication Number | Publication Date |
|---|---|
| CN106957721A true CN106957721A (en) | 2017-07-18 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610895917.1A Pending CN106957721A (en) | 2016-10-13 | 2016-10-13 | A kind of cigarette preparation method of peach extract |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101735999A (en) * | 2010-01-19 | 2010-06-16 | 曹庸 | Method for improving content of resveratrol by converting giant knotweed materials through immobilized enzyme method |
| CN102150948A (en) * | 2011-05-19 | 2011-08-17 | 湖北中烟工业有限责任公司 | Method for preparing jasmine extract for tobacco |
| CN104164453A (en) * | 2014-08-19 | 2014-11-26 | 云南中烟工业有限责任公司 | Peach fermentation broth extract as well as preparation method and application thereof |
-
2016
- 2016-10-13 CN CN201610895917.1A patent/CN106957721A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101735999A (en) * | 2010-01-19 | 2010-06-16 | 曹庸 | Method for improving content of resveratrol by converting giant knotweed materials through immobilized enzyme method |
| CN102150948A (en) * | 2011-05-19 | 2011-08-17 | 湖北中烟工业有限责任公司 | Method for preparing jasmine extract for tobacco |
| CN104164453A (en) * | 2014-08-19 | 2014-11-26 | 云南中烟工业有限责任公司 | Peach fermentation broth extract as well as preparation method and application thereof |
Non-Patent Citations (1)
| Title |
|---|
| 张峻松等: ""超高压处理对桃汁挥发性化学成分的影响"", 《精细化学》 * |
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Application publication date: 20170718 |