CN109770062A - The method and feed addictive of resource utilization wheat vinasse production feed addictive - Google Patents
The method and feed addictive of resource utilization wheat vinasse production feed addictive Download PDFInfo
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- CN109770062A CN109770062A CN201910225975.7A CN201910225975A CN109770062A CN 109770062 A CN109770062 A CN 109770062A CN 201910225975 A CN201910225975 A CN 201910225975A CN 109770062 A CN109770062 A CN 109770062A
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- feed addictive
- enzyme
- wheat vinasse
- resource utilization
- vinasse
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- 238000003825 pressing Methods 0.000 claims description 45
- 238000001784 detoxification Methods 0.000 claims description 25
- 238000002360 preparation method Methods 0.000 claims description 25
- LINOMUASTDIRTM-QGRHZQQGSA-N deoxynivalenol Chemical compound C([C@@]12[C@@]3(C[C@@H](O)[C@H]1O[C@@H]1C=C(C([C@@H](O)[C@@]13CO)=O)C)C)O2 LINOMUASTDIRTM-QGRHZQQGSA-N 0.000 claims description 24
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- HEBKCHPVOIAQTA-NGQZWQHPSA-N d-xylitol Chemical compound OC[C@H](O)C(O)[C@H](O)CO HEBKCHPVOIAQTA-NGQZWQHPSA-N 0.000 claims description 22
- 229930195730 Aflatoxin Natural products 0.000 claims description 20
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- 230000009467 reduction Effects 0.000 claims description 4
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- 238000009834 vaporization Methods 0.000 claims description 4
- 230000008016 vaporization Effects 0.000 claims description 4
- 230000036541 health Effects 0.000 abstract description 13
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- XWIYFDMXXLINPU-UHFFFAOYSA-N Aflatoxin G Chemical compound O=C1OCCC2=C1C(=O)OC1=C2C(OC)=CC2=C1C1C=COC1O2 XWIYFDMXXLINPU-UHFFFAOYSA-N 0.000 description 12
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- 230000001925 catabolic effect Effects 0.000 description 7
- 235000019441 ethanol Nutrition 0.000 description 7
- 150000004676 glycans Chemical class 0.000 description 7
- 239000004615 ingredient Substances 0.000 description 7
- 229920001282 polysaccharide Polymers 0.000 description 7
- 239000005017 polysaccharide Substances 0.000 description 7
- 235000013339 cereals Nutrition 0.000 description 6
- 238000004458 analytical method Methods 0.000 description 5
- 239000006227 byproduct Substances 0.000 description 5
- 238000000855 fermentation Methods 0.000 description 5
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- 231100000614 poison Toxicity 0.000 description 5
- 239000002574 poison Substances 0.000 description 5
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- 238000005259 measurement Methods 0.000 description 3
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- 239000000843 powder Substances 0.000 description 3
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- 239000003053 toxin Substances 0.000 description 3
- 108700012359 toxins Proteins 0.000 description 3
- 241000228197 Aspergillus flavus Species 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- SRBFZHDQGSBBOR-IOVATXLUSA-N D-xylopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 description 2
- 240000000359 Triticum dicoccon Species 0.000 description 2
- 239000000654 additive Substances 0.000 description 2
- 230000000996 additive effect Effects 0.000 description 2
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- 230000014759 maintenance of location Effects 0.000 description 2
- 230000000717 retained effect Effects 0.000 description 2
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- 235000019750 Crude protein Nutrition 0.000 description 1
- 102000035195 Peptidases Human genes 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 206010047700 Vomiting Diseases 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 description 1
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- 238000012262 fermentative production Methods 0.000 description 1
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Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
The present invention discloses a kind of feed addictive that resource utilization wheat vinasse produce the method for feed addictive and are prepared using the production method, wherein, the resource utilization wheat vinasse produce the method for feed addictive the following steps are included: digesting to wheat vinasse, obtain enzymolysis product；The enzymolysis product is used into Ultra filtration membrane, obtains ultrafiltration clear liquid；The ultrafiltration clear liquid is used into nanofiltration UF membrane, obtains nanofiltration retentate fluid；The nanofiltration retentate fluid is concentrated, concentrate is obtained, and the concentrate is dried, obtains feed addictive.Technical solution of the present invention enables to feed addictive to reach national forage health standard.
Description
Technical field
The present invention relates to feed production technology field, in particular to a kind of resource utilization wheat vinasse produce feed addition
The method of agent and the feed addictive being prepared using the production method.
Background technique
Wheat vinasse are usually the byproduct that fermentative production of ethanol obtains, and main component is polysaccharide, albumen and organic acid
Deng.It, can be by carrying out going deep into being added processing to wheat vinasse to obtain wheat since wheat vinasse contain albumen and polysaccharide component
Protein peptides and Xylo-oligosaccharide compounded object, the compound can be used as the additive of feed.But wheat vinasse contain a large amount of biology poison
Element can prevent the feed addictive of production from reaching national health if biotoxin therein cannot be effectively removed
Standard cannot be used directly.
Above content is only used to facilitate the understanding of the technical scheme, and is not represented and is recognized that above content is existing skill
Art.
Summary of the invention
The main object of the present invention is to provide a kind of method of resource utilization wheat vinasse production feed addictive, it is intended to
So that feed addictive reaches national forage health standard.
To achieve the above object, the method for resource utilization wheat vinasse production feed addictive proposed by the present invention, packet
Include following steps:
Wheat vinasse are digested, enzymolysis product is obtained；
The enzymolysis product is used into Ultra filtration membrane, obtains ultrafiltration clear liquid；
The ultrafiltration clear liquid is used into nanofiltration UF membrane, obtains nanofiltration retentate fluid；
The nanofiltration retentate fluid is concentrated, concentrate is obtained, and the concentrate is dried, obtains feed and add
Add agent.
Optionally, the molecular cut off of the nanofiltration membrane is 200Da to 1000Da.
Optionally, described when the nanofiltration retentate fluid is concentrated, control cocnentration factor is 0.65:1 to 0.75:1.
Optionally, described " by the enzymolysis product use Ultra filtration membrane, obtain ultrafiltration clear liquid " the step of include:
Filters pressing operation is carried out to the enzymolysis product, obtains filters pressing clear liquid；
The filters pressing clear liquid is used into Ultra filtration membrane, obtains ultrafiltration clear liquid.
Optionally, the step of described " nanofiltration retentate fluid is concentrated " includes: using reduction vaporization concentration method pair
Nanofiltration retentate fluid is concentrated；And/or
The step of described " concentrate is dried " includes: to be retained using spray drying process to the nanofiltration after concentration
Liquid is dried.
Optionally, the molecular cut off of the ultrafiltration membrane is 5000Da to 30000Da.
Optionally, the step of described " digesting to wheat vinasse, obtain enzymolysis product " includes:
Wheat vinasse are successively carried out with first time enzymatic hydrolysis, is digested for second, enzymolysis product is obtained.
Optionally, when the first time digests, the enzyme preparation of addition is the bacterium enzyme of bacillus and vomitoxin detoxification enzyme
Mix preparation, aflatoxins catabolic enzyme and facultative aerobic character Bifidobacterium, and the enzyme total amount being added is the wheat vinasse quality
0.5%-3%.
Optionally, when second of enzymatic hydrolysis, the enzyme preparation of addition is cellulase, xylo-oligosaccharide enzyme, protease and breaks
Wall enzyme, and the enzyme total amount being added is the 0.1%-0.5% of the wheat vinasse quality.
The invention also provides a kind of feed addictive, the feed addictive is by resource utilization wheat wine as described above
What the method for grain production feed addictive produced.
Technical solution of the present invention, by carrying out enzymatic hydrolysis operation to wheat vinasse stoste, to remove wherein a large amount of biology
Toxin, and enzymolysis product is obtained into ultrafiltration clear liquid using Ultra filtration membrane, ultrafiltration clear liquid is received using nanofiltration membrane is isolated
Trapped fluid is filtered, concentration and drying are successively carried out to nanofiltration retentate fluid can obtain dry feed addictive.Due to wheat wine
Poor stoste has carried out enzymolysis processing, then the feed addictive produced contains less amount of biotoxin, can reach country
Sanitary standard can be added directly into animal feed and carry out nutrition purposes, especially for feeding animals.
Specific embodiment
The technical scheme in the embodiments of the invention will be clearly and completely described below, it is clear that described implementation
Example is only a part of the embodiments of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, this field is general
Logical technical staff every other embodiment obtained without creative efforts belongs to what the present invention protected
Range.
It in addition, the technical solution between each embodiment can be combined with each other, but must be with ordinary skill
Based on personnel can be realized, this technical side will be understood that when the combination of technical solution appearance is conflicting or cannot achieve
The combination of case is not present, also not the present invention claims protection scope within.
The method that the present invention proposes a kind of resource utilization wheat vinasse production feed addictive, the production method include with
Lower step:
Wheat vinasse are digested, enzymolysis product is obtained；
Enzymolysis product is used into Ultra filtration membrane, obtains ultrafiltration clear liquid；
Ultrafiltration clear liquid is used into nanofiltration UF membrane, obtains nanofiltration retentate fluid；
Nanofiltration retentate fluid is concentrated, concentrate is obtained, and concentrate is dried, obtains feed addictive.
Specifically, enzymatic hydrolysis operation is carried out to wheat vinasse stoste first, to remove wherein a large amount of biotoxin；Then will
Enzymolysis product after enzymatic hydrolysis carries out ultra-filtration and separation using ultrafiltration membrane, obtains ultrafiltration clear liquid；Then obtained ultrafiltration clear liquid is used
Nanofiltration membrane carries out nanofiltration separation, obtains nanofiltration retentate fluid；Finally obtained nanofiltration retentate fluid is successively carried out at concentration and drying
Reason, can obtain dry feed addictive, and the main component of the feed addictive is wheat protein peptide and Xylo-oligosaccharide compounded
Object.
Accordingly, it is to be understood that, technical solution of the present invention, by carrying out enzymatic hydrolysis operation to wheat vinasse stoste, to go
Ultrafiltration clear liquid is obtained using Ultra filtration membrane except wherein a large amount of biotoxin, and by enzymolysis product, ultrafiltration clear liquid is used
The isolated nanofiltration retentate fluid of nanofiltration membrane, concentration and drying are successively carried out to nanofiltration retentate fluid can obtain dry feed addition
Agent.Due to having carried out enzymolysis processing to wheat vinasse stoste, then the feed addictive produced contains less amount of biology poison
Element can reach state health standards, can be added directly into animal feed and carry out nutrition purposes, especially for feeding animals.
It should be noted that wheat vinasse stoste is obtained by following steps: by the way that wheat grain is used dry process
Remaining useless fecula after wheat protein powder (gluten) and wheaten starch is obtained, and saccharification enzymatic hydrolysis is carried out to useless fecula and is added
Saccharomyces cerevisiae carries out fermentation process, after being fermented into ethyl alcohol, carries out distillation extraction to ethyl alcohol, remaining fermentation dope is then wheat wine
Poor stoste, solid content is 8%-20% in the wheat vinasse stoste.The step can be realized the reasonable resource of wheat grain
Change and utilize, efficiently avoids the waste of resource.
Optionally, the molecular cut off of nanofiltration membrane is 200Da-1000Da.Contain protein peptides and oligomeric wood in ultrafiltration clear liquid
Sugared ingredient can be operated by nanofiltration and is separated, when carrying out nanofiltration operation, the nanofiltration membrane of Yao Shiyi, preferably will
Protein peptides therein and xylo-oligosaccharide ingredient are separated, to improve finally obtained wheat protein peptide and Xylo-oligosaccharide compounded
The yield of object, namely improve the yield of feed addictive.For example, by using nanofiltration membrane molecular cut off be 200 (dalton)
Da or 500Da or 1000Da.When carrying out nanofiltration operation using the nanofiltration membrane of the range molecular cut off, obtained nanofiltration is retained
Liquid concentration is 18%-25%.
Further, when nanofiltration retentate fluid being concentrated, control cocnentration factor is 0.65:1 to 0.75:1.It is being concentrated
When operation, strict control cocnentration factor is 0.65:1 or 0.7:1 or 0.75:1, namely the solid content after concentration is made to be nanofiltration
The 65%-75% of trapped fluid.The time of subsequent drying can so be reduced, to improve the production efficiency of feed addictive.
Further, by enzymolysis product by Ultra filtration membrane, the step of obtaining ultrafiltration clear liquid, includes:
Filters pressing operation is carried out to enzymolysis product, obtains filters pressing clear liquid；
Filters pressing clear liquid is used into Ultra filtration membrane, obtains ultrafiltration clear liquid.
Since solid content is more in the liquid after wheat vinasse stoste enzymatic hydrolysis, such as directlys adopt ultrafiltration membrane and separated, then
It is possible that the phenomenon that blocking ultrafiltration fenestra, influences the smooth progress of ultrafiltration, therefore filters pressing operation can be first passed through by big portion
It point is separated by solid-liquid separation, moreover, operating to obtain filter cake by filters pressing further in-depth to handle to obtain animal feed, so may make wheat
Vinasse obtain reasonable resource utilization, avoid the wasting of resources.The filters pressing clear liquid operated by filters pressing contains albumen and polysaccharide
Ultra filtration membrane can be used in ingredient, will wherein albumen and polysaccharide component separate.It should be understood that via filters pressing and ultrafiltration
It operates, can not only be effectively separated heterogeneity therein twice, and then obtain different byproducts, and can also make
Ultrafiltration is more smooth and be rapidly performed by.When carrying out filters pressing operation, diaphragm filter press is can be selected in the filter press of use,
Solid content is 9%-12% in the filters pressing clear liquid that filters pressing obtains.
Optionally, the molecular cut off of ultrafiltration membrane is 5000Da to 30000Da.Contain protein peptides and oligomeric in filters pressing clear liquid
Xylose ingredient can be separated by ultrafiltration, when carrying out ultrafiltration, the ultrafiltration membrane of Yao Shiyi, with preferably
Protein peptides therein and xylo-oligosaccharide ingredient are separated, to improve the yield of finally obtained feed addictive.Such as
The molecular cut off of the ultrafiltration membrane used is 5000Da or 10000Da or 20000Da or 30000Da.
Optionally, the step of nanofiltration retentate fluid being concentrated includes: using reduction vaporization concentration method to nanofiltration retentate fluid
It is concentrated.Concentration operation is carried out using concentration method is evaporated under reduced pressure, concentration more effectively can be carried out to nanofiltration retentate fluid,
And make concentration operation more energy saving.Generally, the concentrator that reduction vaporization concentration method uses is mechanical steam recompression
(MVR) device, steam heating power recompression (TVR)+multi-effect evaporating device, Multi-effect concentration equipment, single-action concentrator etc., these are dense
Contracting equipment is energy-saving equipment, and when use enables to concentration operation more energy efficient.
Optionally, the step of concentrate being dried includes: using spray drying process to the nanofiltration retentate fluid after concentration
It is dried.Concentrate is dried using spray drying process, air-flow is enabled to come into full contact with concentrate, that is, increases concentration
The drying area of object, so that drying process is more efficient and quick.
Further, the step of wheat vinasse are digested, obtain enzymolysis product include:
Wheat vinasse are successively carried out with first time enzymatic hydrolysis, is digested for second, enzymolysis product is obtained.
First enzymatic hydrolysis is primarily to remove the biotoxin in wheat vinasse stoste, so that the feed finally prepared
The content of biotoxin reaches state health standards in additive.Second of enzymatic hydrolysis operation is primarily to make wheat vinasse former
The increment of product made from liquid increases the content of the prebiotics such as protein small peptide and xylo-oligosaccharide in final feed addictive obtained,
When the feed addictive is added into animal feed, the palatability and digestibility of feed product can be improved.Wheat is reduced simultaneously
The viscosity of vinasse stoste greatly improves the solid slag separative efficiency of subsequent filters pressing operation.
Optionally, when digesting for the first time, the enzyme preparation of addition is that bacillus mixes with the bacterium enzyme of vomitoxin detoxification enzyme
Preparation, aflatoxins catabolic enzyme and facultative aerobic character Bifidobacterium, and the enzyme total amount being added is the 0.5%- of the wheat vinasse quality
3%.
The bacterium enzyme mix preparation of bacillus and vomitoxin detoxification enzyme, can be by vomitoxin as a kind of feed addictive
In DON be converted into 3-epi-DON, it is the minimum DON of current toxicity that the toxicity of 3-epi-DON, which reduces by 350 times or more compared with DON,
Metabolite can also efficiently remove vomitoxin therein.Aflatoxins catabolic enzyme and facultative aerobic character Bifidobacterium are total to
Same-action can preferably remove aflatoxin therein.Also, the enzyme content being added in the first enzymatic hydrolysis will be suitable for, so that
The first time enzymatic hydrolysis detoxification for obtaining wheat vinasse is more thorough, to more efficiently remove vomitoxin therein and aspergillus flavus poison
Element, for example, for the first time enzymatic hydrolysis be added enzyme total amount be wheat vinasse 0.5% or 0.7% or 1% perhaps 2% or
2.5% or 3%.
It should be noted that one of the various enzyme preparation additional amounts will also fit when preparing the enzyme that enzymatic hydrolysis is added for the first time
Preferably, to give full play to the enzymatic hydrolysis detoxifications of various enzymes.Optionally, bacillus mixes system with the bacterium enzyme of vomitoxin detoxification enzyme
The mass ratio of agent, aflatoxins catabolic enzyme and facultative aerobic character Bifidobacterium is (3-6): (2-5): (0.5-2).
Also, strict control is needed to digest operating condition well when enzymatic hydrolysis detoxification for the first time, to guarantee enzymatic hydrolysis operation more
It is effectively performed.For example, 3 or 5 or 6 or 6.5 or 7 or 7.5, hydrolysis temperature is controlled at 20 DEG C or 30 for pH value control when enzymatic hydrolysis
DEG C or 40 DEG C or 50 DEG C；The enzyme digestion reaction time is 2h or 3h or 4h or 5h or 6h or 7h or 8h.
If lower than 3, perhaps hydrolysis temperature is lower than 20 DEG C or the enzyme digestion reaction time is less than 8h for pH value when enzymatic hydrolysis, then can to digest
Process is not thorough enough, causes finally obtained feed product content of toxins that cannot reach sanitary index.If if pH value when enzymatic hydrolysis
Higher than 7.5, perhaps hydrolysis temperature is higher than 50 DEG C or the enzyme digestion reaction time is higher than 8h, then can make the entire enzymolysis process time
It is longer, so that production efficiency is lower.
Optionally, second when digesting, and the enzyme preparation of addition is cellulase, xylo-oligosaccharide enzyme, protease and wall breaking enzyme,
And the enzyme total amount being added is the 0.1%-0.5% of the wheat vinasse quality.
After cellulase and xylo-oligosaccharide enzyme are added, under its synergistic effect, the crude fibre in vinasse stoste can reduce,
The content for increasing the soluble polysaccharides such as polyxylan and xylo-oligosaccharide in product, moreover, polyxylan therein is in digestive system
In it is degradable be xylo-oligosaccharide, for xylo-oligosaccharide as a kind of prebiotics, content is higher, activity it is higher, be more advantageous to beneficial bacterium
Growth so that final products realize increment.Polyoses content is higher in product simultaneously, can be improved feed product palatability and
Digestibility.Also, the viscosity that can also reduce vinasse stoste easily causes subsequent filters pressing since polysaccharide is relatively easy to binding protein
It operates and the filter opening of filter press is blocked, therefore reduce the viscosity of vinasse, filter opening blocks when filters pressing operation can be effectively avoided
The phenomenon that, so that filters pressing operation is gone on smoothly, improve its production efficiency.
The addition of protease can be improved the content of soluble protein in vinasse stoste, while can also improve final be made
Feed product in wheat protein peptide content, to improve the palatability and digestibility of feed product.
The addition of wall breaking enzyme, can the yeast that stays of broken wall fermentation alcohol, sporoderm-broken rate can be improved to 95% or more, increases
Add the content of original yeast extract.Also, it is acted on proteinase synergy, more protein peptides can be extracted.Simultaneously as complete
Whole yeast cells is also easy to the filter opening of blocking filter press, and also results in the increase of vinasse stoste viscosity, also can be effective after broken wall
The phenomenon that filter opening blocks when ground avoids filters pressing from operating improves its production efficiency so that filters pressing operation is gone on smoothly.
Also, the enzyme content being added in the second enzymatic hydrolysis will be suitable for, so that second of enzymatic hydrolysis of wheat vinasse is more thorough
Bottom, to reduce the viscosity of vinasse, more effectively to guarantee that the production efficiency of subsequent filters pressing operation, such as second of enzymatic hydrolysis are added
Enzyme total amount be wheat vinasse 0.1% or 0.2% or 0.3% or 0.4% or 0.5%.
When preparing the enzyme that second of enzymatic hydrolysis is added, one of the various enzyme preparation additional amounts also will be suitable for, to give full play to
Various enzymatic hydrolysis detoxifications.Optionally, the mass ratio of cellulase, xylo-oligosaccharide enzyme, protease and wall breaking enzyme is (1-5):
(10-30):(20-50):(1-10)。
And second is also required to strict control when digesting and digests operating condition well, to guarantee that enzymatic hydrolysis operation is more efficient
Ground carries out.For example, 3 or 5 or 6.5 or 7 or 7.5, hydrolysis temperature is controlled at 50 DEG C or 60 DEG C or 75 for pH value control when enzymatic hydrolysis
℃；The enzyme digestion reaction time is 8h perhaps 10h or 12h.If pH value is lower than 3 when enzymatic hydrolysis or hydrolysis temperature is lower than 50 DEG C,
Or the enzyme digestion reaction time is less than 8h, then enzymolysis process can be made not thorough enough, finally obtained feed product toxin is caused to contain
Amount cannot reach sanitary index.If if higher than 7.5, perhaps hydrolysis temperature is higher than 75 DEG C or enzyme digestion reaction to pH value when enzymatic hydrolysis
Time is higher than 12h, then the entire enzymolysis process time can be made longer, so that production efficiency is lower.
It should be noted that the sequence of enzymatic hydrolysis and second of enzymatic hydrolysis cannot exchange for the first time, because most harmful thin
The survival temperature of bacterium is 60 DEG C hereinafter, the operation that needs to cool down if first carrying out second digesting, will cause germ contamination, and pass through
Second of enzymatic hydrolysis is carried out again after enzymatic hydrolysis detoxification for the first time, and temperature is increased to 70 DEG C, and most of harmful bacteria is dead, then
Final feed product pollution is not will cause.
Of course, other also can be selected with identical function or similar function in the enzyme that enzymatic hydrolysis and second of enzymatic hydrolysis are added for the first time
Can enzyme preparation substitute.
The invention also provides a kind of feed addictive, which is by resource utilization wheat as described above
What the method for vinasse production feed addictive produced.
Feed addictive is produced using wheat vinasse to the present invention below by way of specific embodiment and feed addictive carries out
It is described in detail.
Embodiment 1
Step 1, by remaining after using dry process to obtain wheat protein powder (gluten) and wheaten starch wheat grain
Useless fecula, and to wheat flour give up fecula carry out saccharification enzymatic hydrolysis and be added saccharomyces cerevisiae carry out fermentation process, be fermented into second
After alcohol, distillation extraction is carried out to ethyl alcohol, remaining fermentation dope is then wheat vinasse stoste.
Step 2, is added the first enzyme preparation that mass fraction is 0.5% in Xiang little Mai vinasse stoste, and in pH value be 7.5,
Hydrolysis temperature is 20 DEG C, enzymatic hydrolysis detoxification for the first time is carried out under the operating condition that the enzyme digestion reaction time is 8h, to remove wheat vinasse
Biotoxin in stoste.Wherein, the first enzyme preparation is bacterium enzyme mix preparation, the Huang Qu of bacillus and vomitoxin detoxification enzyme
Mycin catabolic enzyme and facultative aerobic character Bifidobacterium, and bacterium enzyme mix preparation, the aflatoxins of bacillus and vomitoxin detoxification enzyme
Catabolic enzyme and the mass ratio of facultative aerobic character Bifidobacterium are 6:5:2.
Step 3, be subsequently added into mass fraction be 0.1% the second enzyme preparation, and in pH value be 7.5, hydrolysis temperature 50
DEG C, second is carried out under the operating condition that the enzyme digestion reaction time is 8h and is digested, to obtain enzymolysis product, wherein the second enzyme preparation is
Cellulase, xylo-oligosaccharide enzyme, protease and wall breaking enzyme, and the quality of cellulase, xylo-oligosaccharide enzyme, protease and wall breaking enzyme
Than for 1:10:20:1.
Enzymolysis product is put into progress filters pressing operation in filter press, obtains filters pressing clear liquid by step 4.
The filters pressing clear liquid of acquisition is used molecular cut off for the Ultra filtration membrane of 5000Da, it is clear to obtain ultrafiltration by step 5
Liquid.
The ultrafiltration clear liquid of acquisition is used molecular cut off for the nanofiltration UF membrane of 200Da by step 6, obtains nanofiltration retention
Liquid.
Step 7 successively carries out nanofiltration retentate fluid concentration is evaporated under reduced pressure and spray-on process is dry, obtains dry protein peptides
With Xylo-oligosaccharide compounded object namely feed addictive.
In the operating process using wheat vinasse production feed addictive, it is former that the vinasse before and after detoxification are digested to first time
The content of vomitoxin and aflatoxin is detected in liquid, and testing result is shown in Table 1.
Table 1 digests the content detection result of vomitoxin and aflatoxin in the vinasse stoste before and after detoxification for the first time
Illustrate: detection method is the detection method in national examination criteria, similarly hereinafter.
As can be seen from Table 1, after first time digests detoxification, vomitoxin and aflatoxin can effectively be gone
It removes, and content meets vomitoxin content≤5mg/ in feedstuff specified in national forage health standard GB13078-2017
Kg (5000 μ g/kg), aflatoxin minimum require to be≤10 μ g/kg.
Similarly, also the viscosity of the vinasse stoste before and after second of enzymatic hydrolysis detoxification is detected, testing result is shown in
Table 2.
The viscosity measurements result of the vinasse stoste of second of table 2 enzymatic hydrolysis front and back
As can be seen from Table 2, after the second enzymolysis processing, the viscosity of wheat vinasse stoste is substantially reduced, and is had after being convenient for
It is continuous to be separated by solid-liquid separation operation, its separative efficiency is improved, to improve the yield of final byproduct.
Further, the time for carrying out filters pressing operation to the material after not digesting material and digesting respectively is detected,
Testing result is shown in Table 3.
Table 3 do not digest and digest after time for being operated through filters pressing of material
| Analysis project | Detected value (h) | Detection method |
| Material is not digested | 12 | Internal timer method |
| Digested material | 3 | Internal timer method |
As can be seen from Table 3, since the viscosity of vinasse stoste after enzymatic hydrolysis greatly reduces, then material is pressed after digesting
The filter operation time used greatly shortens, so as to improve its filters pressing operating efficiency.
Meanwhile water content is examined in the clear liquid to the material after not digesting material and digesting after filters pressing operates respectively
It surveys, testing result is shown in Table 4.
Table 4 do not digest and digest after material water content in clear liquid after filters pressing operates
As shown in Table 4, water-soluble substances increase in the filters pressing clear liquid after being digested operation and filters pressing operation twice, are conducive to
Subsequent ultrafiltration.
Finally, the feed addictive ingredient obtained to production tests and analyzes, detection and analysis the results are shown in Table 5.
5 feed addictive composition detection of table analyzes result
| Analysis project | Testing result | Measurement unit | Detection method |
| Moisture | 5 | % | GB/T6435-2014 |
| Coarse ash | 4.7 | % | GB/T6438-2007 |
| Amino-acid nitrogen | 0.66 | % | GB/T23530-2009/6.5 |
| Crude protein | 36.68 | % | GB/T6432-1994 |
| Small peptide | 32.17 | % | GB/T22492-2008 |
| Total reducing sugar | 31.42 | % | / |
| Xylo-oligosaccharide | 11.69 | % | GB/T23747-2009 |
| Vomitoxin | 750 | μg/kg | The first method of GB5009.111-2016/ |
| Aflatoxin | It is not detected | μg/kg | GB/T30955-2014 |
As can be seen from Table 5, the main component for the feed addictive which produces is albumen and polysaccharide component,
And wherein in the vomiting that contains, content is lower, meets national forage health standard (total vomitoxin content≤5000 of feedstuff
μg/kg)；And the content of aflatoxin is not detected, then the feed addictive that present invention production obtains meets national health mark
Standard can directly carry out nutrition purposes, especially for feeding animals.
Embodiment 2
Step 1, by remaining after using dry process to obtain wheat protein powder (gluten) and wheaten starch wheat grain
Useless fecula, and to wheat flour give up fecula carry out saccharification enzymatic hydrolysis and be added saccharomyces cerevisiae carry out fermentation process, be fermented into second
After alcohol, distillation extraction is carried out to ethyl alcohol, remaining concentrate is then wheat vinasse stoste.
Step 2, is added the first enzyme preparation that mass fraction is 3% in Xiang little Mai vinasse stoste, and in pH value be 3, enzymatic hydrolysis
Temperature is 50 DEG C, enzymatic hydrolysis detoxification for the first time is carried out under the operating condition that the enzyme digestion reaction time is 2h, to remove wheat vinasse stoste
In biotoxin.Wherein, the first enzyme preparation is bacterium enzyme mix preparation, the aflatoxins of bacillus and vomitoxin detoxification enzyme
Catabolic enzyme and facultative aerobic character Bifidobacterium, and the bacterium enzyme mix preparation of bacillus and vomitoxin detoxification enzyme, aflatoxins decompose
Enzyme and the mass ratio of facultative aerobic character Bifidobacterium are 3:2:0.5.
Step 3, be subsequently added into mass fraction be 0.5% the second enzyme preparation, and in pH value be 3, hydrolysis temperature 75
DEG C, second is carried out under the operating condition that the enzyme digestion reaction time is 12h and is digested, to obtain enzymolysis product, wherein the second enzyme preparation
For cellulase, xylo-oligosaccharide enzyme, protease and wall breaking enzyme, and the matter of cellulase, xylo-oligosaccharide enzyme, protease and wall breaking enzyme
Amount is than being 5:30:30:10.
Enzymolysis product is put into progress filters pressing operation in filter press, obtains distiller's grains liquor by step 4.
The filters pressing clear liquid of acquisition is used molecular cut off for the Ultra filtration membrane of 10000Da, it is clear to obtain ultrafiltration by step 5
Liquid.
The ultrafiltration clear liquid of acquisition is used molecular cut off for the nanofiltration UF membrane of 700Da by step 6, obtains nanofiltration retention
Liquid.
Step 7 successively carries out nanofiltration retentate fluid concentration is evaporated under reduced pressure and spray-on process is dry, obtains dry protein peptides
With Xylo-oligosaccharide compounded object namely feed addictive.
Similarly, the present embodiment digests first time in the operating process using wheat vinasse production feed addictive
The content of vomitoxin and aflatoxin is detected in vinasse stoste before and after detoxification, and testing result is shown in Table 6.
Table 6 digests the content detection result of vomitoxin and aflatoxin in the vinasse stoste before and after detoxification for the first time
Illustrate: detection method is the detection method in national examination criteria, similarly hereinafter.
As can be seen from Table 6, after first time digests detoxification, vomitoxin and aflatoxin can effectively be gone
It removes, and content meets vomitoxin content≤5mg/ in feedstuff specified in national forage health standard GB13078-2017
Kg (5000 μ g/kg), aflatoxin minimum require to be≤10 μ g/kg.
Similarly, also the viscosity of the vinasse stoste before and after second of enzymatic hydrolysis detoxification is detected, testing result is shown in
Table 7.
The viscosity measurements result of the vinasse stoste of second of table 7 enzymatic hydrolysis front and back
As can be seen from Table 7, after the second enzymolysis processing, the viscosity of wheat vinasse stoste is substantially reduced, and is had after being convenient for
It is continuous to be separated by solid-liquid separation operation, its separative efficiency is improved, to improve the yield of final byproduct.
Further, the time for carrying out filters pressing operation to the material after not digesting material and digesting respectively is detected,
Testing result is shown in Table 8.
Table 8 do not digest and digest after time for being operated through filters pressing of material
| Analysis project | Detected value (h) | Detection method |
| Material is not digested | 12 | Internal timer method |
| Digested material | 3 | Internal timer method |
As can be seen from Table 8, since the viscosity of vinasse stoste after enzymatic hydrolysis greatly reduces, then material is pressed after digesting
The filter operation time used greatly shortens, so as to improve its filters pressing operating efficiency.
Meanwhile water content is examined in the clear liquid to the material after not digesting material and digesting after filters pressing operates respectively
It surveys, testing result is shown in Table 9.
Table 9 do not digest and digest after material water content in clear liquid after filters pressing operates
As shown in Table 9, water-soluble substances increase in the filters pressing clear liquid after being digested operation and filters pressing operation twice, are conducive to
Subsequent ultrafiltration.
Finally, testing and analyzing to the ingredient for obtaining feed addictive, detection and analysis the results are shown in Table 10.
10 feed addictive composition detection of table analyzes result
As can be seen from Table 10, the main component for the feed addictive which produces is albumen, protein peptides, more
Sugar and oligosaccharide composition, and the vomitoxin content contained is lower, meeting national forage health standard, (feedstuff always vomits poison
Cellulose content≤5000 μ g/kg)；And the content of aflatoxin is not detected, then the feed addictive that present invention production obtains is multiple
State health standards are closed, can be used directly as feed addictive.
Therefore, the method for resource utilization wheat vinasse production feed addictive of the present invention, by wheat vinasse stoste
Operation is successively digested twice, and the first enzymatic hydrolysis detoxification can remove vomitoxin and aspergillus flavus poison in wheat vinasse stoste
Element can reach so that containing less amount of biotoxin in the subsequent byproduct that wheat vinasse are goed deep into working process
State health standards can be used directly.Second of enzymatic hydrolysis can increase containing for the prebiotics such as its protein small peptide and xylo-oligosaccharide
Amount, meanwhile, the viscosity of wheat vinasse stoste can be also reduced, and operate convenient for later separation, improve separative efficiency.And to enzyme twice
Enzymolysis product after solution successively carries out filters pressing and ultra-filtration and separation obtains ultrafiltration clear liquid, and ultrafiltration clear liquid progress nanofiltration separation is received
Filter trapped fluid, and to nanofiltration retentate fluid successively carry out concentration and drying process produce to obtain feed addictive, the feed addictive
Containing less amount of biotoxin, state health standards can be reached, can be added directly in animal feed and use.
The above description is only a preferred embodiment of the present invention, is not intended to limit the scope of the invention, all at this
Under the inventive concept of invention, using equivalent structure transformation made by present specification, or directly/it is used in other indirectly
Relevant technical field is included in scope of patent protection of the invention.
Claims (10)
1. a kind of method of resource utilization wheat vinasse production feed addictive, which is characterized in that the production method include with
Lower step:
Wheat vinasse are digested, enzymolysis product is obtained；
The enzymolysis product is used into Ultra filtration membrane, obtains ultrafiltration clear liquid；
The ultrafiltration clear liquid is used into nanofiltration UF membrane, obtains nanofiltration retentate fluid；
The nanofiltration retentate fluid is concentrated, concentrate is obtained, and the concentrate is dried, obtains feed addition
Agent.
2. the method for resource utilization wheat vinasse production feed addictive as described in claim 1, which is characterized in that described
The molecular cut off of nanofiltration membrane is 200Da to 1000Da.
3. the method for resource utilization wheat vinasse production feed addictive as described in claim 1, which is characterized in that institute
When stating nanofiltration retentate fluid and being concentrated, control cocnentration factor is 0.65:1 to 0.75:1.
4. the method for resource utilization wheat vinasse production feed addictive as described in claim 1, which is characterized in that described
" by the enzymolysis product use Ultra filtration membrane, obtain ultrafiltration clear liquid " the step of include:
Filters pressing operation is carried out to the enzymolysis product, obtains filters pressing clear liquid；
The filters pressing clear liquid is used into Ultra filtration membrane, obtains ultrafiltration clear liquid.
5. the method for resource utilization wheat vinasse production feed addictive as described in claim 1, which is characterized in that described
The step of " nanofiltration retentate fluid is concentrated " includes: that nanofiltration retentate fluid is concentrated using reduction vaporization concentration method；
And/or
The step of described " concentrate is dried " include: using spray drying process to the nanofiltration retentate fluid after concentration into
Row drying.
6. the method for resource utilization wheat vinasse production feed addictive as described in claim 1, which is characterized in that described
The molecular cut off of ultrafiltration membrane is 5000Da to 30000Da.
7. special such as the method for resource utilization wheat vinasse described in any one of claims 1 to 6 production feed addictive
The step of sign is, described " digesting to wheat vinasse, obtain enzymolysis product " include:
Wheat vinasse are successively carried out with first time enzymatic hydrolysis, is digested for second, enzymolysis product is obtained.
8. the method for resource utilization wheat vinasse production feed addictive as claimed in claim 7, which is characterized in that described
When digesting for the first time, the enzyme preparation of addition is the bacterium enzyme mix preparation of bacillus and vomitoxin detoxification enzyme, aflatoxins point
Enzyme and facultative aerobic character Bifidobacterium are solved, and the enzyme total amount being added is the 0.5%-3% of the wheat vinasse quality.
9. the method for resource utilization wheat vinasse production feed addictive as claimed in claim 7, which is characterized in that described
Second when digesting, and the enzyme preparation of addition is cellulase, xylo-oligosaccharide enzyme, protease and wall breaking enzyme, and the enzyme total amount being added
For the 0.1%-0.5% of the wheat vinasse quality.
10. a kind of feed addictive, which is characterized in that the feed addictive is as described in any one of claims 1 to 9
What the method for resource utilization wheat vinasse production feed addictive produced.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111282550A (en) * | 2020-03-03 | 2020-06-16 | 武汉轻工大学 | Preparation method and application of load type magnetic cellulose microspheres |
| CN114380925A (en) * | 2021-12-30 | 2022-04-22 | 武汉美味源生物工程有限公司 | Method for drying corn distillers' grains and extracting soluble polysaccharide |
| CN115005328A (en) * | 2022-07-01 | 2022-09-06 | 东莞益海嘉里生物科技有限公司 | Vinasse stock solution treatment and reuse method |
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| CN107988271A (en) * | 2017-12-14 | 2018-05-04 | 安徽瑞福祥食品有限公司 | A kind of processing method of wheat containing vomitoxin and application |
| CN109452539A (en) * | 2018-11-14 | 2019-03-12 | 陈雨 | The biodegrading process of mycotoxin and less toxic fermentative feedstuff of microbe in corn and wheat and its converted products |
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| CN104187174A (en) * | 2014-09-15 | 2014-12-10 | 中国科学院青岛生物能源与过程研究所 | Process for producing livestock feed additive from reside and dreg materials |
| CN107988271A (en) * | 2017-12-14 | 2018-05-04 | 安徽瑞福祥食品有限公司 | A kind of processing method of wheat containing vomitoxin and application |
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| CN111282550A (en) * | 2020-03-03 | 2020-06-16 | 武汉轻工大学 | Preparation method and application of load type magnetic cellulose microspheres |
| CN114380925A (en) * | 2021-12-30 | 2022-04-22 | 武汉美味源生物工程有限公司 | Method for drying corn distillers' grains and extracting soluble polysaccharide |
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