CN101214004B - Method for using rough material containing vegetable fibre to produce feed - Google Patents
Method for using rough material containing vegetable fibre to produce feed Download PDFInfo
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- CN101214004B CN101214004B CN2007103085393A CN200710308539A CN101214004B CN 101214004 B CN101214004 B CN 101214004B CN 2007103085393 A CN2007103085393 A CN 2007103085393A CN 200710308539 A CN200710308539 A CN 200710308539A CN 101214004 B CN101214004 B CN 101214004B
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Abstract
The invention discloses a feed production method with the raw material containing plant fiber. The invention is carried out as follows, Bacillus licheniformis BL57 CGMCC No. 2277 and/or Bacillus licheniformis BL54 CGMCC No. 2276 are inoculated into the raw material containing the plant fiber and fermented in the temperature of 30 to 50 DEG C, then is dried to obtain the feed. Straw and other agricultural wastes as well as industrial fermentation waste such as lees are utilized to be fermented and produce the feed, in the invention, wastes are fully utilized, the large quantity of the cellulose in the wastes are converted into reducing sugar( the converted reducing sugar can reach 90 percent of that of the corn flour). Therefore, the invention can not only increase the value of wastes, butalso enable the produced feed to have the function of being beneficial to digest and the comprehensive nutrition.
Description
Technical field
The present invention relates to a kind of production method of feed, particularly relate to the method that a kind of utilization contains the raw material production feed of string.
Background technology
String class materials such as cellulose, hemicellulose and lignin are prevalent in the middle of the plant, because their characteristic itself, if as feed, its ratio that is absorbed and used is very low, therefore often are taken as rubbish and meet with discarded.
Only taking annual gramineae Zea plant corn is example, and the vitality of this crop is very indomitable, except that extreme sandy soil and clay, all can cultivate.Investigation shows, the corn that every production is 100 kilograms can produce 17.5 kilograms corn ear (corn husk) and corn stigma, the corncob of 100 kilograms cornstalk and 41 kilograms.Calculate as can be known, the total amount of byproducts such as cornstalk and corncob is 158.5% of principal yield, is a quite surprising numeral.But generally speaking, because the composition of cornstalk and corncob is difficult to be utilized, so they often have been taken as discarded object, and its value only is 3.4% of principal product.
At present, because the price of cereal products such as corn and soya bean improves constantly, the someone proposes, and some is rich in cellulosic plant processed side product adds in the middle of the animal feed.But its effect is unsatisfactory.Because, in feed, directly add and be rich in cellulosic plant processed side product, though the cost of feed can be reduced, but because animal is lower to the digestive utilization ratio of these plant processed side products, causing feeding cost not obtain corresponding synchronous reduces, its consequence that directly causes is exactly that the brood incubation rate descends, and the stage of fattening, the getting fat effect of fowl poultry was relatively poor, so use amount has been subjected to considerable restraint.Current only small part is used for mixing with clover, makes piece, and it is edible to supply with livestocks such as ox, sheep, horse.
The world energy sources shortage makes using yeast bacterium or other peculiar microorganism that high starch cereal is changed into alcohol and waits and provide the technical scheme of the energy to arise at the historic moment, and is corresponding, and how its byproduct that brings is used also becomes the very urgent problem that solves of needing.For the country that abounds with corn, Chinese sorghum, paddy rice, wheat, barley, cassava, the byproduct that is rich in string is very various, wood chip the like waste as rice straw, cornstalk, broomcorn straw, straw and forestry processing factory, its cost of material is lower, and not influenced by staple food supply and price, have very large exploitation and be worth.
The value that how to improve high fiber cereal one maize straw the like waste will be following big business opportunity, and improve the digestibility of string, at first will convert it into oligosaccharides or monose.
Microorganism is the source that nature decomposes the enzyme maximum of plant cellulose, and industrial enzymes is produced used material generally all from microorganism at present.
Summary of the invention
The purpose of this invention is to provide the method that a kind of utilization contains the raw material production feed of string.
Utilization provided by the present invention contains the method for the raw material production feed of string, be that bacillus licheniformis (Bacillus licheniformis) BL57 CGMCC № .2277 and/or bacillus licheniformis (Bacilluslicheniformis) BL54 CGMCC № .2276 are inoculated in the raw material that contains string, 30-50 ℃ of fermentation, drying obtains feed.
In the described method, described fermentation pH value is 6-9, is preferably pH6.5.
In the described method, described fermentation temperature is preferably 45 ℃.
In the described method, the described raw material that contains string can be agriculture waste plant tissue or the trade waste that contains string, as raw materials such as straw, plant leaf blade, commercial fermentation waste thing such as vinasse.
In the described method, the inoculum concentration of described bacillus licheniformis (Bacillus licheniformis) BL57 CGMCC № .2277 or bacillus licheniformis (Bacillus licheniformis) BL54 CGMCC № .2276 can be 10
6More than the cfu/g raw material, be preferably 10
6-10
7The cfu/g raw material.
In the described method, described fermentation time can be 24-48 hour.
Described bacillus licheniformis (Bacillus licheniformis) BL54 CGMCC № .2276, separate certainly from the Taichung County,Taiwan Province soil sample, be preserved in Chinese microorganism strain preservation board of trustee reason person on November 30th, 2007 and understand common micro-organisms center (abbreviation CGMCC, the address is: Da Tun road, Chaoyang District, BeiJing, China city), preserving number is CGMCC № .2276.
Bacillus licheniformis (Bacillus licheniformis) BL57 CGMCC № .2277, separate certainly from the Taichung County,Taiwan Province soil sample, be preserved in Chinese microorganism strain preservation board of trustee reason person on November 30th, 2007 and understand common micro-organisms center (abbreviation CGMCC, the address is: Da Tun road, Chaoyang District, BeiJing, China city), preserving number is CGMCC № .2277.
Identify by enzymatic activity, show that bacillus licheniformis (Bacillus licheniformis) BL54 CGMCC № .2276 and bacillus licheniformis (Bacillus licheniformis) BL57 CGMCC № .2277 all have cellulose hydrolysis enzymatic activity, hydrolase of proteolysis and amylase activity.
Method of the present invention, utilize agricultural wastes such as straw and industrial fermentation process discarded object (as vinasse) to ferment with bacillus licheniformis (Bacillus licheniformis) BL57, bacillus licheniformis (Bacillus licheniformis) BL54, make feed, made full use of discarded object, convert the cellulose in the discarded object to reduced sugar (conversion ratio reaches 90%) in a large number, not only increased the value of discarded object greatly, and the feed of making have effects such as the digestion of being beneficial to, comprehensive nutrition.
Advantage with the bacillus licheniformis fermentation has: bacillus licheniformis has been the gas bacillus, and the multiple ferment of quick justacrine of growing can decompose fiber wastes, protein and starch etc., further is converted to and can utilizes carbohydrate.This bacterium is that environment comes derived bacterium in addition, and nonpathogenic bacteria and do not have cause of disease harm do not have anxiety for food security.
The specific embodiment
Method among the following embodiment if no special instructions, is conventional method.
Percentage composition among the following embodiment if no special instructions, is the quality percentage composition.
Method of the present invention has been utilized two bacterial strains, be respectively bacillus licheniformis (Bacillus licheniformis) BL54 CGMCC № .2276 and bacillus licheniformis (Bacillus licheniformis) BL57 CGMCC № .2277, preparation method is as described below:
Respectively get a gram soil from Taichung County,Taiwan Province soil sample three place's diverse locations, add 10mlTrypticase respectively
TM(every liter contains tryptone (Tryptose) 20.0g to Soy Broth (TSB nutrient solution), glucose (Dextrose) 1.0g, sodium hydrogen phosphate (Disodium Phosphate) 2.0g, potassium nitrate (Potassium Nitrate) 1.0g) after concussion mixes in, (every liter contains peptone (Peptone) 1.0g to use the Mandels-Reese culture medium respectively, carboxymethyl cellulose (CMC, Carboxymethyl cellulose) 10.0g, (NH
4)
2SO
41.4g, Urea0.3g, K
2HPO
42.0g, CaCl
2.H
2O0.4g, MgSO
4.7H
2O0.3g, FeSO
4.7H
2O5.0mg, MnSO
4.H
2O1.6mg, ZnSO
4.7H
2O1.4mg, CoCl
2.7H
2O2.0mg transfers to 7.0 with pH, and solid medium then adds 1.5%Agar) carry out 10 times of gradient dilutions to 10
-10Divide respectively in 4 dilution tubes of packing into, each gradient dilution liquid is incubated at 30 ℃, 40 ℃, 50 ℃ different temperatures respectively cultivated 24 hours, the bacterium that tentatively increases with the active bacterium of cellulase is screened.Get 100 μ l to TSA (Tryptic soy agar from each dilution tube again, Difco, Sparks, U.S.A.) on the flat board, and smear evenly with L type glass rod, place 30 ℃ equally respectively, 40 ℃, cultivate after 24 hours for 50 ℃, again with on the new TSA of the single bacterium colony to of picked at random mode picking, and scratch with three-step approach, after the repetition purifying subculture three times, on 40 ℃ of flat boards, obtain the bacterial strain that two strains have cellulase activity and proteinase activity, be bacillus licheniformis (Bacilluslicheniformis) BL54 CGMCC № .2276 and bacillus licheniformis (Bacillus licheniformis) BL57CGMCC № .2277.
The physiological and biochemical property of bacillus licheniformis (Bacillus licheniformis) BL54 CGMCC № .2276 is as shown in table 1:
The physiological and biochemical property of table 1, bacillus licheniformis (Bacillus licheniformis) BL54
| BL54 | BL54 | ||
| Bacterium colony size (mm) | 6-8 | Metabolite | |
| Colony shape | Irregular | Melitose | + |
| Color | In vain | D-sorbite | (+) |
| Gram | + | Trehalose | + |
| Spread during cultivation | - | Maltose | + |
| Nitrate reduction | + | The black bearberry foline | + |
| Catalase | + | Rough gentian second candy | + |
| Growth | Glucose | + | |
| 50C | + | Galactolipin | + |
| 5％NaCl | + | Lactose | - |
| Metabolite | Sucrose | + | |
| Dextrin | + | Salicin | + |
| Glycogen | + | Wood sugar | + |
| Acetamido glucose | + | Malic acid | + |
| Arabic glycocoll | - | Glycerine | + |
| Cellobiose | + | Alanine | + |
| Synanthrin | - | The silk amino acid | + |
In the table 1 ,-: feminine gender; +: the positive; (+): the weak positive; ND: do not identify;
Identify by enzymatic activity, show that bacillus licheniformis (Bacillus licheniformis) BL54 CGMCC № .2276 has cellulose hydrolysis enzymatic activity, hydrolase of proteolysis and amylase activity.
Described bacillus licheniformis (Bacillus licheniformis) BL54 CGMCC № .2276, be preserved in Chinese microorganism strain preservation board of trustee reason person on November 30th, 2007 and understand common micro-organisms center (abbreviation CGMCC, the address is: Da Tun road, Chaoyang District, BeiJing, China city), preserving number is CGMCC № .2276.
The physiological and biochemical property of bacillus licheniformis (Bacillus licheniformis) BL57 CGMCC № .2277 is as shown in table 2:
The physiological and biochemical property of table 2, bacillus licheniformis (Bacillus licheniformis) BL57
| BL57 | ?BL57 | ||
| Bacterium colony size (mm) | 6-8 | Metabolite | |
| Colony shape | Irregular | Melitose | + |
| Color | In vain | D-sorbite | (+) |
| Gram | + | Trehalose | + |
| Spread during cultivation | - | Maltose | + |
| Nitrate reduction | + | The black bearberry foline | + |
| Catalase | + | Rough gentian second candy | + |
| Growth | Glucose | + | |
| 50℃ | + | Galactolipin | + |
| 5％NaCl | + | Lactose | - |
| Metabolite | Sucrose | + | |
| Dextrin | + | Salicin | + |
| Glycogen | + | Wood sugar | + |
| Acetamido glucose | + | Malic acid | + |
| Arabic glycocoll | - | Glycerine | + |
| Cellobiose | + | Alanine | + |
| Synanthrin | - | The silk amino acid | + |
In the table 2 ,-: feminine gender; +: the positive; (+): the weak positive; ND: do not identify;
Identify by enzymatic activity, show that bacillus licheniformis (Bacillus licheniformis) BL57 CGMCC № .2277 has cellulose hydrolysis enzymatic activity, hydrolase of proteolysis and amylase activity.
Bacillus licheniformis (Bacillus licheniformis) BL57 CGMCC № .2277, be preserved in Chinese microorganism strain preservation board of trustee reason person on November 30th, 2007 and understand common micro-organisms center (abbreviation CGMCC, the address is: Da Tun road, Chaoyang District, BeiJing, China city), preserving number is CGMCC № .2277.
Embodiment 1, utilization contain the method and the effect detection thereof of the raw material production feed of string
Present embodiment bacillus licheniformis (Bacillus licheniformis) BL54 CGMCC № .2276, with sunflower dish powder, straw, green bean straw powder, millet bar powder, sunflower pole powder, maize cob meal, cornstalk or vinasse is raw material, carry out the fermenting and producing feed, concrete grammar is as described below:
With 500ml beaker dress raw material, scale 10g sunflower dish powder, straw, green bean straw powder, millet bar powder, sunflower pole powder, maize cob meal, cornstalk or vinasse are raw material respectively, add 10 respectively in each beaker
6The bacillus licheniformis of cfu/g raw material (Bacillus licheniformis) BL54 CGMCC № .2276, and (ddwater, DDW), after mixing, regulating the pH value is 6.5, is statically placed in 45 ℃ of insulating boxs, ferments 24 hours to add the 50ml deionized water.
Sunflower dish powder, straw, green bean straw powder, millet bar powder, sunflower pole powder, maize cob meal, cornstalk or vinasse after the fermentation are tiled on the stainless steel disc, and thickness is thinner better, puts 60 ℃ of baking ovens blowing oven dry, to moisture be below 8%.Pulverize the raw material that is feed with electric crusher, can be used for replacing in the pig chicken cattle and sheep complete diet pellet corn flour (starch) raw material.
Before and after the fermentation, measure fermenting raw materials front and back composition according to following method respectively:
(1) analysis of crude protein content
With smart 0.5 gram that claims of sample powder, put into and decompose pipe, and add the 15ml concentrated sulfuric acid (8mol/L) and 1 catalyst (Kjeltab catalyst), with crude protein analyzer (B-435, B ü chi, Switzerland) hot digestion decomposed 2 hours, till the solution clarification.After the cooling, add 50ml distilled water, with crude protein distilling apparatus (B-316, B ü chi, Switzerland) distillation digestive juice, and be that 4% BAS adds 2 mixed indicators (methyl red: methyl blue=3: 2) as receiving liquid, distillation time is 5 minutes with the mass percentage concentration of 50ml, receive liquid with the titration of 0.1mol/LHCl solution again, when color is titration end-point when rose pink by emerald green becoming.Record HCl drips of solution is quantitative, and calculates crude protein content (%) with following formula.Experimental repeatability: each sample is got two parallel samples and is measured, and is the result with its arithmetic mean of instantaneous value; When the thick protein mass content more than 25%, allowing relative deviation is 1%; When the thick protein mass content at 10-25%, allowing relative deviation is 2%; When the thick protein mass content below 10%, allowing relative deviation is 3%.
Among the formula I, a: with the used milliliter number of 0.1mol/L HCl drips of solution random sample product; B: with the blank used milliliter number of 0.1mol/LHCl titration; 6.25: nitrogenous coefficient; 0.0014:1ml0.1mol/LHCl solution is equivalent to the nitrogen amount of 0.0014g; HCl demarcates the power valency of concentration/0.1:0.1mol/LHCl solution.
(2) analysis of crude fat content
With smart 1 gram that claims of sample powder, parcel is gone into the filter paper of constant weight (Whatman No.1), put into the reflux condensing tube of Soxhlet device again, after 8 hours, take out filter paper with reflux extraction with absolute ether, place the cabinet of bleeding to make the ether volatilization, again with infrared moisture meter (IR-200, Derner Instrument Company USA) is dried to filter paper constant weight and write down weight fast, calculates crude fat content (%) with following formula at last.
(3) determination of moisture
Weigh sample and place in the weighing bottle for about 5 g, in 105 ℃ to 110 ℃ drying boxes, dry, weighing to the constant, its decrement is moisture.
Moisture (%)=(A/B) * 100
A=is dried decrement (g), B=sample weight (g)
Or with infrared moisture meter (IR-200, Denver Instrument Company USA) measure its moisture, and with 24 hours data average after, obtain the moisture (%) of ight soil.
(4) crude fiber content is measured
Weigh sample 2g, extracting the back with ether puts in the flask together with asbestos 0.5g, adding 200ml sulfuric acid liquid (flask and cooler are direct) heats immediately, must make solution boiling in the bottle in 1 minute, rotated flask once every 5 minutes, can fully mix in order to do solution in the bottle, and must note to be stained with sample on the interior liquid level of bottle.Be blown into air with foam-expelling, boil after 30 minutes and filter it with the Gooch crucible of putting the asbestos thin layer, with the boiling water flushing, to not containing till the acidity the inferior sodium hydroxide solution 200ml that uses, sediment and asbestos are washed in the bottle that flashes back, removed flask in 30 minutes with boiling after cooler is connected, filter with original Gooch crucible again, with the boiling water flushing till do not contain acidity, use 95% alcohol 15ml again, wash it with ether 10ml then.Put Gooch crucible and in be dissolved in the baking oven with 110 ± 2 ℃ of oven dry of temperature, after cooling scale it.Move again on the ashing stove burn approximately to about 20 minutes braised in soy sauce removing carbon elimination, after the cooling again scale it, calculate the weight of being lost, be the crude fibre amount.
Crude fibre (%)=(A/B) * l00
A=ash content decrement (g), B=sample weight (g)
(5) coarse ash is measured
Weighing sample 5 to 10g is in porcelain crucible, and scorching hot to constant weight in 600 ℃ of temperature, residual amount is coarse ash.
Coarse ash (%)=(A/B) * 100
A=residue weight (g), B=sample weight (g)
(6) fermentation back carbohydrate generates and analyzes
Assay method is with Miller, and the 1959 DNS reducing sugar test methods of being developed are measured, and compare with glucose preparation standard curve.The total reducing sugars amount is the contained reduced sugar amount of 1 gram raw material, try to achieve the reduced sugar amount (discharging reduced sugar amount=experimental group (raw material after the fermentation) reduced sugar amount one control group (not fermentation raw material) reduced sugar amount) that the enzyme effect discharges through the formula conversion, and with the original reduced sugar amount of each raw material in contrast, to detect its utilization rate and effect effectiveness.
Sunflower dish powder, straw, green bean straw powder, millet bar powder, sunflower pole powder, maize cob meal, cornstalk or vinasse raw material Main Ingredients and Appearance, the fiber content analysis result is as shown in table 3 below:
Composition before table 3. fermenting raw materials
| Kind | Thick protein % | Crude fat % | Coarse ash % | Crude fibre % |
| Cornstalk | 6.2 | 2.3 | 5.3 | 23.4 |
| Corncob | 4.2 | 2.8 | 1.8 | 23.8 |
| Vinasse | 25.3 | 5.3 | 3.4 | 56.8 |
| Sunflower dish powder | 7.45 | 3.32 | 8.66 | 15.43 |
| Straw | 1.67 | 1.04 | 5.94 | 38.80 |
| Green bean straw powder | 7.09 | 0.68 | 12.56 | 33.16 |
| Millet bar powder | 5.4 | 1.28 | 5.12 | 28.28 |
| The sunflower pole powder | 2.42 | 0.78 | 4.09 | 40.92 |
It is as shown in table 4 that different material is handled back reduced sugar generation.Reduced sugar Growth Rate Calculation formula in the table 4 is:
The reduced sugar generation of the feed that table 4. different material obtains after fermentation process
| Different material | 1g raw material total reducing sugars amount (mg) before the effect | Total reducing sugars amount (mg) after the effect of 1g raw material | Reduced sugar growth rate (%) | (%) renderd a service in effect * |
| Sunflower dish powder | 80 | 100.6 | 25.8 | 81.8 |
| Straw | 42.5 | 69.2 | 62.8 | 56.2 |
| Green bean straw powder | 51.5 | 79.3 | 53.9 | 64.5 |
| Millet bar powder | 58.3 | 83.4 | 43.1 | 67.8 |
| The sunflower pole powder | 38.0 | 68.2 | 79.5 | 55.5 |
| Maize cob meal | 69.2 | 115.0 | 66.2 | 93.5. |
| Cornstalk | 46.5 | 91.0 | 95.7 | 75.6％ |
| Vinasse | 14.9 | 45.3 | 204.0 | 38.5％ |
* effect effectiveness is 100% with the original reduced sugar amount of corn flour, with the total reducing sugars amount after the fermenting raw materials effect divided by corn flour reduced sugar amount (123mg) promptly.
Embodiment 2, utilization contain the method and the effect detection thereof of the raw material production feed of string
Present embodiment bacillus licheniformis (Bacillus licheniformis) BL57 CGMCC № .2277, with sunflower dish powder, straw, green bean straw powder, millet bar powder, sunflower pole powder, maize cob meal, cornstalk or vinasse is raw material, carry out the fermenting and producing feedstuff, concrete grammar is as described below:
With 500ml beaker dress raw material, scale 10g sunflower dish powder, straw, green bean straw powder, millet bar powder, sunflower pole powder, maize cob meal, cornstalk or vinasse are raw material respectively, add 10 respectively in each beaker
7The bacillus licheniformis of cfu/g raw material (Bacillus licheniformis) BL57 CGMCC № .2277, and add the 50ml deionized water, after mixing, regulating the pH value is 6.5, is statically placed in 45 ℃ of insulating boxs, ferments 36 hours.
Sunflower dish powder, straw, green bean straw powder, millet bar powder, sunflower pole powder, maize cob meal, cornstalk or vinasse after the fermentation are tiled on the stainless steel disc, and thickness is thinner better, puts 60 ℃ of baking ovens blowing oven dry, to moisture be below 8%.Pulverize the raw material that is feed with electric crusher, can be used for replacing in the pig chicken cattle and sheep complete diet pellet corn flour (starch) raw material.
Sunflower dish powder, straw, green bean straw powder, millet bar powder, sunflower pole powder, maize cob meal, cornstalk or vinasse raw material, its Main Ingredients and Appearance, fiber content analysis result are as shown in table 3, calculate different material reduced sugar generation after fermentation process according to embodiment 1 described method, shown in the table 5, the reduced sugar Growth Rate Calculation formula in the table 5 is as a result:
The reduced sugar generation of the feed that table 5. different material obtains after fermentation process
| Different material | 1g raw material total reducing sugars amount (mg) before the effect | Total reducing sugars amount (mg) b after the effect of 1g raw material | Reduced sugar growth rate (%) | (%) renderd a service in effect * |
| Sunflower dish powder | 80 | 98.3 | 22.9 | 79.9 |
| Straw | 42.5 | 67.3 | 58.3 | 54.7 |
| Green bean straw powder | 51.5 | 77.4 | 50.3 | 62.9 |
| Millet bar powder | 58.3 | 83.1 | 42.5 | 67.6 |
| The sunflower pole powder | 38 | 66.5 | 75.0 | 54.1 |
| Corncob | 69.2 | 100.7 | 45.5 | 81.9 |
| Cornstalk | 46.5 | 86.4 | 85.8 | 70.2 |
| Vinasse | 14.9 | 41.4 | 177.6 | 33.7 |
* effect effectiveness is 100% with the original reduced sugar amount of corn flour, with total reducing sugars amount after the fermenting raw materials effect divided by corn flour reduced sugar amount (123mg) promptly.
Embodiment 3, utilization contain the method and the effect detection thereof of the raw material production feed of string
Present embodiment is a raw material with bacillus licheniformis (Bacillus licheniformis) BL54 CGMCC № .2276 sunflower dish powder, straw, green bean straw powder, millet bar powder or sunflower pole powder, carries out the fermenting and producing feed, and concrete grammar is as described below:
With 500ml beaker dress raw material, scale 10g sunflower dish powder, straw, green bean straw powder, millet bar powder, sunflower pole powder, maize cob meal, cornstalk or vinasse are raw material respectively, add 10 respectively in each beaker
6The bacillus licheniformis of cfu/g raw material (Bacillus licheniformis) BL57 CGMCC № .2277 and 10
6The bacillus licheniformis of cfu/g raw material (Bacillus licheniformis) BL54 CGMCC № .2276, and add the 50ml deionized water, after mixing, regulating the pH value is 6.5, is statically placed in 45 ℃ of insulating boxs, ferments 48 hours.
Sunflower dish powder, straw, green bean straw powder, millet bar powder, sunflower pole powder, maize cob meal, cornstalk or vinasse after the fermentation are tiled on the stainless steel disc, and thickness is thinner better, puts 60 ℃ of baking ovens blowing oven dry, to moisture be below 8%.Pulverize the raw material that is feed with electric crusher, can be used for replacing in the pig chicken cattle and sheep complete diet pellet corn flour (starch) raw material.
Sunflower dish powder, straw, green bean straw powder, millet bar powder, sunflower pole powder, maize cob meal, cornstalk or vinasse raw material, its Main Ingredients and Appearance, the fiber content analysis result is as shown in table 3, calculate different material reduced sugar generation after fermentation process according to embodiment 1 described method, the result is as shown in table 6.Reduced sugar Growth Rate Calculation formula in the table 6 is:
The reduced sugar generation of the feed that table 6. different material obtains after fermentation process
| Kind | 1g raw material total reducing sugars amount (mg) before the effect | Total reducing sugars amount (mg) after the effect of 1g raw material | Reduced sugar growth rate (%) | (%) renderd a service in effect * |
| Corncob | 69.2 | 110.7 | 60.0 | 90％ |
| Cornstalk | 46.5 | 93.0 | 100 | 75％ |
| Vinasse | 14.9 | 47.3 | 217 | 38％ |
| Sunflower dish powder | 80.0 | 100.6 | 25.8 | 82％ |
| Straw | 42.5 | 59.0 | 38.9 | 48％ |
| Green bean straw powder | 51.5 | 69.3 | 34.6 | 56％ |
| Millet bar powder | 58.3 | 74.2 | 27.3 | 60％ |
| The sunflower pole powder | 38.0 | 60.2 | 58.4 | 49％ |
* effect effectiveness is 100% with the original reduced sugar amount of various raw materials, with total reducing sugars amount after the fermenting raw materials effect divided by the original reduced sugar amount of raw material (123mg) promptly.
The result of the foregoing description shows, utilize agricultural wastes such as straw and industrial fermentation process discarded object (as vinasse) to ferment, make feed, made full use of discarded object, convert the cellulose in the discarded object to reduced sugar (the reduced sugar conversion ratio reaches as high as more than 90%) in a large number, not only increased the value of discarded object greatly, and the feed of making have effects such as the digestion of being beneficial to, comprehensive nutrition.
Claims (6)
1. a utilization contains the method that the raw material of string is produced feed, be that bacillus licheniformis (Bacilluslicheniformis) BL57 CGMCC № .2277 and/or bacillus licheniformis (Bacillus licheniformis) BL54CGMCC № .2276 are inoculated in the raw material that contains string, 30-50 ℃ of fermentation, drying obtains feed;
The pH value condition of described fermentation is pH6-pH 8;
Described fermentation time is 24-48 hour.
2. method according to claim 1 is characterized in that: the pH value condition of described fermentation is pH6.5.
3. method according to claim 2 is characterized in that: the temperature of described fermentation is 45 ℃.
4. method according to claim 3 is characterized in that: the described raw material that contains string is straw, plant leaf blade, wood chip and/or vinasse.
5. method according to claim 4, it is characterized in that: in the described method, the inoculum concentration of described bacillus licheniformis (Bacillus licheniformis) BL57 CGMCC № .2277 or bacillus licheniformis (Bacilluslicheniformis) BL54 CGMCC № .2276 is 10
6More than the cfu/g raw material.
6. method according to claim 5 is characterized in that: the inoculum concentration of described bacillus licheniformis (Bacilluslicheniformis) BL57 CGMCC № .2277 or bacillus licheniformis (Bacillus licheniformis) BL54CGMCC № .2276 is 10
6-10
7The cfu/g raw material.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2007103085393A CN101214004B (en) | 2007-12-29 | 2007-12-29 | Method for using rough material containing vegetable fibre to produce feed |
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|---|---|---|---|
| CN2007103085393A CN101214004B (en) | 2007-12-29 | 2007-12-29 | Method for using rough material containing vegetable fibre to produce feed |
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| CN101214004A CN101214004A (en) | 2008-07-09 |
| CN101214004B true CN101214004B (en) | 2011-03-30 |
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| US10006073B2 (en) * | 2013-05-24 | 2018-06-26 | Chr, Hansen A/S | Use of Bacillus composition for increasing the amount of available sugars in animal feed |
| CN108013251A (en) * | 2017-12-29 | 2018-05-11 | 苏州帝凯维动物营养有限公司 | A kind of pannage containing crude fibre |
| CN110384176B (en) * | 2019-08-23 | 2021-04-20 | 华中农业大学 | Method for preparing animal feed by culturing bacillus licheniformis through distiller's grains and puffed corn flour |
| CN111837808B (en) * | 2020-07-24 | 2022-06-03 | 福建农林大学 | Method for efficiently recovering and treating pine wood nematode disease wood |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1232628A (en) * | 1999-02-08 | 1999-10-27 | 王德民 | Production process of microbial protein feed |
| CN1520739A (en) * | 2002-02-26 | 2004-08-18 | 东莞市豪发生物工程开发有限公司 | Bacilli fungi mixed fermentation for producing microbial formulation and compound enzyme feed additive |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1232628A (en) * | 1999-02-08 | 1999-10-27 | 王德民 | Production process of microbial protein feed |
| CN1520739A (en) * | 2002-02-26 | 2004-08-18 | 东莞市豪发生物工程开发有限公司 | Bacilli fungi mixed fermentation for producing microbial formulation and compound enzyme feed additive |
Non-Patent Citations (3)
| Title |
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| 张民.微生态制剂在饲料工业中的应用.中国饲料.2003,(16),17-19. * |
| 石剑华等.秸秆生物饲料对反刍家畜的营养作用.吉林畜牧兽医.2007,(1),25-26. * |
| 罗建成等.利用多菌体混合发酵转化玉米秸秆的研究.微生物学杂志.2007,27(6),107-110. * |
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