CN105961854A - Preparation method of corn straw feed - Google Patents
Preparation method of corn straw feed Download PDFInfo
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- CN105961854A CN105961854A CN201610304931.XA CN201610304931A CN105961854A CN 105961854 A CN105961854 A CN 105961854A CN 201610304931 A CN201610304931 A CN 201610304931A CN 105961854 A CN105961854 A CN 105961854A
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- 239000010902 straw Substances 0.000 title claims abstract description 84
- 235000002017 Zea mays subsp mays Nutrition 0.000 title claims abstract description 30
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 title claims abstract description 29
- 235000005822 corn Nutrition 0.000 title claims abstract description 29
- 238000002360 preparation method Methods 0.000 title claims abstract description 28
- 240000008042 Zea mays Species 0.000 title claims description 28
- 238000000855 fermentation Methods 0.000 claims abstract description 32
- 230000004151 fermentation Effects 0.000 claims abstract description 32
- 238000000034 method Methods 0.000 claims abstract description 22
- 238000010298 pulverizing process Methods 0.000 claims abstract description 14
- 241001465754 Metazoa Species 0.000 claims abstract description 12
- 238000010025 steaming Methods 0.000 claims abstract description 11
- 150000001875 compounds Chemical class 0.000 claims abstract description 10
- 108090000790 Enzymes Proteins 0.000 claims abstract description 7
- 102000004190 Enzymes Human genes 0.000 claims abstract description 7
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 18
- 244000286779 Hansenula anomala Species 0.000 claims description 16
- 241000894006 Bacteria Species 0.000 claims description 13
- 108010059892 Cellulase Proteins 0.000 claims description 12
- 101710121765 Endo-1,4-beta-xylanase Proteins 0.000 claims description 12
- 229940106157 cellulase Drugs 0.000 claims description 12
- 229940059442 hemicellulase Drugs 0.000 claims description 12
- 108010002430 hemicellulase Proteins 0.000 claims description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 11
- 239000000843 powder Substances 0.000 claims description 10
- 239000004310 lactic acid Substances 0.000 claims description 9
- 235000014655 lactic acid Nutrition 0.000 claims description 9
- 210000000582 semen Anatomy 0.000 claims description 8
- 240000006024 Lactobacillus plantarum Species 0.000 claims description 7
- 235000013965 Lactobacillus plantarum Nutrition 0.000 claims description 7
- 229940072205 lactobacillus plantarum Drugs 0.000 claims description 7
- 229930006000 Sucrose Natural products 0.000 claims description 6
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 6
- 239000005720 sucrose Substances 0.000 claims description 6
- 241000235646 Cyberlindnera jadinii Species 0.000 claims description 5
- 235000014683 Hansenula anomala Nutrition 0.000 claims description 5
- 238000001035 drying Methods 0.000 claims description 5
- 229940088598 enzyme Drugs 0.000 claims description 5
- 239000008187 granular material Substances 0.000 claims description 5
- 238000002156 mixing Methods 0.000 claims description 5
- 239000008363 phosphate buffer Substances 0.000 claims description 5
- 239000002054 inoculum Substances 0.000 claims description 4
- 239000000203 mixture Substances 0.000 claims description 3
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 2
- 238000005516 engineering process Methods 0.000 claims description 2
- 238000003860 storage Methods 0.000 claims description 2
- 239000000835 fiber Substances 0.000 abstract description 9
- 108090000623 proteins and genes Proteins 0.000 abstract description 6
- 102000004169 proteins and genes Human genes 0.000 abstract description 6
- 235000015097 nutrients Nutrition 0.000 abstract description 5
- 239000000126 substance Substances 0.000 abstract description 5
- 229920002488 Hemicellulose Polymers 0.000 abstract description 4
- 239000001913 cellulose Substances 0.000 abstract description 4
- 229920002678 cellulose Polymers 0.000 abstract description 4
- 235000016709 nutrition Nutrition 0.000 abstract description 4
- 241000283690 Bos taurus Species 0.000 abstract description 3
- 241001494479 Pecora Species 0.000 abstract description 3
- 230000001954 sterilising effect Effects 0.000 abstract description 2
- 238000004659 sterilization and disinfection Methods 0.000 abstract description 2
- 241000209149 Zea Species 0.000 abstract 2
- 230000015556 catabolic process Effects 0.000 abstract 1
- 238000006731 degradation reaction Methods 0.000 abstract 1
- 244000063299 Bacillus subtilis Species 0.000 description 9
- 235000014469 Bacillus subtilis Nutrition 0.000 description 9
- 235000019750 Crude protein Nutrition 0.000 description 5
- 230000000050 nutritive effect Effects 0.000 description 5
- 239000002253 acid Substances 0.000 description 4
- 235000013339 cereals Nutrition 0.000 description 4
- 239000001888 Peptone Substances 0.000 description 3
- 108010080698 Peptones Proteins 0.000 description 3
- 238000009360 aquaculture Methods 0.000 description 3
- 244000144974 aquaculture Species 0.000 description 3
- 239000003599 detergent Substances 0.000 description 3
- 239000012153 distilled water Substances 0.000 description 3
- 239000001963 growth medium Substances 0.000 description 3
- 229920005610 lignin Polymers 0.000 description 3
- 230000007935 neutral effect Effects 0.000 description 3
- 150000007524 organic acids Chemical class 0.000 description 3
- 235000019629 palatability Nutrition 0.000 description 3
- 235000019319 peptone Nutrition 0.000 description 3
- 241000589220 Acetobacter Species 0.000 description 2
- 208000000412 Avitaminosis Diseases 0.000 description 2
- 241000222120 Candida <Saccharomycetales> Species 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- 206010021135 Hypovitaminosis Diseases 0.000 description 2
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
- 240000007594 Oryza sativa Species 0.000 description 2
- 235000007164 Oryza sativa Nutrition 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 235000015278 beef Nutrition 0.000 description 2
- 230000000593 degrading effect Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000009629 microbiological culture Methods 0.000 description 2
- 208000030401 vitamin deficiency disease Diseases 0.000 description 2
- 241000193755 Bacillus cereus Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241000186660 Lactobacillus Species 0.000 description 1
- 241000235648 Pichia Species 0.000 description 1
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 1
- 241001052560 Thallis Species 0.000 description 1
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 description 1
- 239000002154 agricultural waste Substances 0.000 description 1
- 238000000498 ball milling Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 235000013351 cheese Nutrition 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 235000013325 dietary fiber Nutrition 0.000 description 1
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 239000010977 jade Substances 0.000 description 1
- 229940039696 lactobacillus Drugs 0.000 description 1
- 238000002386 leaching Methods 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 235000019341 magnesium sulphate Nutrition 0.000 description 1
- 235000009973 maize Nutrition 0.000 description 1
- 229940099596 manganese sulfate Drugs 0.000 description 1
- 239000011702 manganese sulphate Substances 0.000 description 1
- 235000007079 manganese sulphate Nutrition 0.000 description 1
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 1
- 229920000053 polysorbate 80 Polymers 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- 239000001632 sodium acetate Substances 0.000 description 1
- 235000017281 sodium acetate Nutrition 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- YWYZEGXAUVWDED-UHFFFAOYSA-N triammonium citrate Chemical compound [NH4+].[NH4+].[NH4+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O YWYZEGXAUVWDED-UHFFFAOYSA-N 0.000 description 1
- 239000001393 triammonium citrate Substances 0.000 description 1
- 235000011046 triammonium citrate Nutrition 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/169—Plantarum
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Fodder In General (AREA)
Abstract
The invention relates to a preparation method of a corn straw feed. The preparation method comprises the following steps of treating corn straw through a mechanical pulverization technique, performing high-temperature steaming on the pulverized straw so as to realize the purposes of performing sterilization and softening fibers, performing treatment through a compound enzyme preparation, and performing fermentation treatment through various microbe strains. According to the preparation method disclosed by the invention, degradation of cellulose and hemicellulose in the straw is realized through various manners, the quantity of nutrient substances, such as protein, in the feed, is increased, the nutrient value of the straw is increased, and nutritional requirements of animals such as cattle and sheep are met.
Description
Technical field
The invention belongs to technique for producing feed field, be specifically related to a kind of corn stalk with better nutritivity value
The preparation method of stalk feed.
Background technology
China's agricultural straw resource is the abundantest, and annual production reaches more than 800,000,000 ton, straw kind mainly with Semen Maydis,
Oryza sativa L. and Semen Tritici aestivi are main, and wherein corn straw occupancy volume is maximum.Corn straw is mainly by cellulose
(31%～40%), hemicellulose (35%～48%) and three kinds of material compositions of lignin (15%～25%).Crude protein contains
Measuring low, vitamin deficiency, nutritive value is the highest.Therefore, corn straw has become main agricultural wastes,
The discarded phenomenon of burning of China's vast rural area straw is extremely serious, and this not only causes the huge waste of resource,
And add atmospheric pollution, make China's haze weather make the matter worse, seriously run counter to China's sustainable development
Theory.
Meanwhile, China, due to the fast development of aquaculture, causes feedstuff shortage.The grain resource of China is very
Limited, the high speed development of aquaculture, cause people and animals and strive the rise of grain and grain price, cause aquaculture simultaneously
Cost remain high.Corn straw is owing to mainly containing cellulose, hemicellulose and lignin, crude protein
Content is low, vitamin deficiency, thus is the roughage that a kind of nutritive value is the highest.Therefore, by modern skill
Art means, improve the nutritive value of straw, have great importance.It is possible not only to realize the height of corn straw
Effect utilizes, and can alleviate the feedstuff grain demand of China, and asks for solving the environment of crop straw burning
Topic, has certain meaning.
At present, having employing fermentable, the method corn straw such as mechanical treatment carries out the method processed, and
Achieve certain effect.But the feedstuff produced still cannot meet the nutritional need of animal, still needs to other and raises
Expect with the use of.
Summary of the invention
It is an object of the invention to provide the preparation method of a kind of corn straw feedstuff, the method passes through enzymolysis and two
Step fermentation can effectively improve the nutritive value of corn straw, and the corn straw feedstuff produced can directly be raised
Feed animal, it is not necessary to add other nutrients again.
To achieve these goals, the technical solution used in the present invention is as follows:
The present invention provides the preparation method of a kind of corn straw feedstuff, comprises the steps:
1) Mechanical comminution techniques is used to process corn straw, a diameter of 0.2-1mm of granule after pulverizing；
2) straw after pulverizing is put in container, and through high temperature steaming 30-60min, the straw after steaming and decocting is spread out
Cooling, this step is for sterilization and softening fibre；
3) compound enzymic preparation is dissolved in water, mixes with straw, carry out room temperature enzymolysis 5-6h；
4), after enzymolysis terminates, two-step fermenting is used to ferment；
5) by the straw feed fermented directly for pulverizing storage after animal edible or drying.
Preferably, described Mechanical comminution techniques includes using blade type pulverizer, beater grinder or ball milling powder
Broken machine is pulverized.
Preferably, described compound enzyme enzyme preparation is made up of xylanase, cellulase and hemicellulase,
Addition is: xylanase 200-400U/g, cellulase 50-100U/g and hemicellulase 50-100
U/g。
Preferably, described two-step fermenting is: 1) one time fermentation: the 1% of addition straw dry matter weight
Semen Maydis powder, adds bacillus subtilis and yeast, and inoculum concentration by volume mass ratio calculates each addition 2.5%,
28 DEG C of-32 DEG C of fermentation process 48-192 hour；2) ferment in second time: after one time fermentation terminates, adds straw and does
The sucrose of the 0.1%-1% of substance weight and the phosphate buffer of the pH 4.0-5.0 of 1%, mixing, then connect
Plant and measure the lactic acid bacteria fermentation that by volume mass ratio adds 5%, 35-37 DEG C of fermentation process 48-96 hour.
Preferably, described yeast is Candida utilis or Hansenula anomala.
Preferably, described lactic acid bacteria is Lactobacillus plantarum.
Run into cold snap during enzymolysis, can use and heat or prolong long mode, if ambient temperature fall
Low to 15-20 DEG C, 24h can be extended to, if temperature is less than 15 DEG C, then be difficult to enzymolysis, need to take
Measure improves ambient temperature.
Present invention is characterized in that
Using xylanase in the present invention, cellulase, hemicellulase can be with the fibre in degrading maize straws
Dimension element, hemicellulose and lignin, obtain the small-molecular-weight sugar that animal can effectively utilize.
Add Semen Maydis powder when one time fermentation and primarily serve the purpose of withered bacillus cereus and yeast extra-nutrition material,
Hay spore can produce multiple enzyme, can polymer substance in degrading straw further；Adding yeast can
To utilize the small-molecule substance of generation of being degraded by enzymolysis and bacillus subtilis, further synthesising thalli protein,
Improve the protein content of straw.
Adding sucrose when ferment in second time and be chiefly to facilitate the growth of lactic acid bacteria, lactic acid bacteria is in growth and breeding process
In available sugar produce organic acid (lactic acid), make organic acid content raise, the mouthfeel of feedstuff, straw can be improved
Palatability be obviously improved, straw feed conversion ratio improves, and organic acid can substitute for feeding antibiotic and press down
Harmful microbe processed grows, and improves the body immunity of domestic animal, prevents and treat intestinal tract disease.
Complex enzyme zymohydrolysis is separated with fermentable by the present invention, is owing to enzymolysis is different from fermentation condition,
If carried out, it is impossible to obtain optimum efficiency simultaneously.Particularly fermentation stage, also especially by bacillus subtilis,
Yeast separately ferments with lactic acid bacteria, is owing to lactic acid bacteria fermentation produces acid, can cause suppressing hay spore
Bacillus and saccharomycetic growth, cause fermenting unsuccessfully.After Fen Kai, be conducive to the pH etc. by regulating straw
Method, makes rapid microbial growth.
Strain used by the present invention, all buys from Chinese industrial Microbiological Culture Collection administrative center (CICC).
Wherein bacillus subtilis, numbered CICC 10088；Candida utilis, numbered CICC 1314；
Hansenula anomala, numbered CICC 32257；Lactobacillus plantarum, numbered CICC 21794.
The beneficial effects of the present invention is:
The present invention provides the preparation method of a kind of corn straw feedstuff, and the method uses enzymolysis and ferment in second time
In conjunction with processing, it is effectively improved the content of the nutrient substance such as protein in corn straw, thus improves jade
The nutritive value of rice straw, it is not necessary to add other nutrients again, and palatability is strong, meet cattle and sheep etc. and move
The nutritional need of thing.
Detailed description of the invention
Technical scheme is further illustrated below by detailed description of the invention.Described embodiment is only
It is to aid in understanding the present invention, is not construed as the concrete restriction to the present invention.
Xylanase used by the present invention, cellulase and hemicellulase are all bought to be had from DSM (Chinese)
Limit company.
Strain used by the present invention, all buys from Chinese industrial Microbiological Culture Collection administrative center (CICC).
Wherein bacillus subtilis, numbered CICC 10088；Candida utilis, numbered CICC 1314；
Hansenula anomala, numbered CICC 32257；Lactobacillus plantarum, numbered CICC 21794.
The condition of culture of microorganism used therefor is:
Bacillus subtilis culture medium prescription: peptone 5.0g, beef leaching thing 3.0g, NaCl 5.0g, steam
Distilled water 1.0L, pH7.0.Condition of culture, 200 revs/min, cultivates bacterium to OD by 37 DEG C600To 1.0.
Yeast (Candida utilis or Hansenula anomala) culture medium prescription: peptone 20g, yeast
Powder 10g, glucose 10g, distilled water 1.0L, pH7.0, condition of culture, 30 DEG C, 200 revs/min, will
Bacterium is cultivated to OD600To 1.0.
Lactobacillus plantarum culture medium: cheese peptone 10.0g, beef powder 8.0g, yeast powder 4.0g, glucose 20.0g,
Magnesium sulfate 0.2g, sodium acetate 5.0g, Triammonium citrate 2.0g, dipotassium hydrogen phosphate 2.0g, manganese sulfate 0.05g,
Tween 80 1.0g, distilled water 1L, pH6.2 ± 0.2.Condition of culture, 200 revs/min, trains bacterium by 37 DEG C
Support to OD600 to 1.0.
" volume mass ratio " of the present invention is L/kg, and such as, adding volume mass than the bacterium solution being 5% is
0.05L joins in 1kg, or equal proportion change.
Embodiment 1:
(1) using the mode of ball mill pulverizing to process corn straw, after pulverizing, the diameter of granule is at 0.2mm.
(2) straw after pulverizing is put in container, and through high temperature steaming 45min, the straw after steaming and decocting is spread out
Cool.
(3) compound enzymic preparation is dissolved in water, mixes with straw, carry out room temperature enzymolysis 5h.
The consumption of water with straw hold agglomerating, unclamp and i.e. dissipate for most preferably.
Compound enzymic preparation includes: xylanase, cellulase and hemicellulase, and consumption is:
The addition of enzyme preparation is:
Xylanase: 400U/g；
Cellulase: 50U/g；
Hemicellulase: 50U/g；
(4), after enzymolysis terminates, two-step fermenting is used to ferment；Detailed process is: 1) one time fermentation:
The Semen Maydis powder of the 1% of addition straw dry matter weight, adds bacillus subtilis (CICC 10088) and produces
Each 2.5% (the volume mass ratio) of protein candida mycoderma (CICC 1314), 30 degree, fermentation process 48 hours；
2) ferment in second time: after one time fermentation terminates, add straw dry matter weight 0.5% sucrose and 1%
The phosphate buffer of pH5.0, mixing；It is subsequently adding Lactobacillus plantarum (CICC 21794) fermentation inoculation
Amount be 5% (volume mass than), 37 DEG C of fermentation process 48 hours.
(5) by the straw feed fermented directly for preserving after animal edible or drying.
After fermentation ends, the neutral detergent fiber in measuring and calculating straw is down to 62.3% by 67.1%, and crude fibre is by 27%
Being down to 21.4%, crude protein is increased to 8.32% by 6.78%, and in straw, total acid content reaches 2.91%.
Embodiment 2:
(1) using the mode of hammer type crushing to process corn straw, after pulverizing, the diameter of granule is at 0.5mm.
(2) straw after pulverizing is put in container, high temperature steaming 35min), the straw after steaming and decocting is spread out
Cool.
(3) compound enzymic preparation is dissolved in water, mixes with straw, carry out enzymolysis 5h.
The consumption of water with straw hold agglomerating, unclamp and i.e. dissipate for most preferably.
Compound enzymic preparation includes: xylanase, cellulase and hemicellulase, and consumption is:
The addition of enzyme preparation is:
Xylanase: 200U/g
Cellulase: 100U/g
Hemicellulase: 100U/g
(4), after enzymolysis terminates, two-step fermenting is used to ferment；Detailed process is: 1) one time fermentation:
Add straw dry matter weight 1% Semen Maydis powder, add bacillus subtilis (CICC 10088) and different
Often Hansenula yeast (CICC 32257) each 2.5% (volume mass than), 30 degree of fermentation process 96 hours；2)
Ferment in second time: after one time fermentation terminates, the sucrose of the 1% of addition straw dry matter weight and the pH value of 1%
It is the phosphate buffer of 4.5, mixing；It is subsequently adding Lactobacillus plantarum (CICC 21794), sends out for 35 DEG C
Ferment processes 96 hours.
(5) by the straw feed fermented directly for preserving after animal edible or drying.
After fermentation ends, the neutral detergent fiber in straw is down to 63.1% by 67.1%, and crude fibre is by 27% fall
To 20.9%, crude protein is increased to 7.92% by 6.78%, and in straw, total acid content reaches 2.79%.
Embodiment 3:
(1) mode using blade type to pulverize processes corn straw, a diameter of 1mm of granule after pulverizing.
(2) straw after pulverizing is put in container, and high temperature steaming 30min, the straw after steaming and decocting is spread out and put
Cool.
(3) being dissolved in water by compound enzymic preparation, mix with straw, room temperature carries out enzymolysis 6h.
The consumption of water with straw hold agglomerating, unclamp and i.e. dissipate for most preferably.
Compound enzymic preparation includes: xylanase, cellulase and hemicellulase, and consumption is:
Xylanase: 300U/g
Cellulase: 80U/g
Hemicellulase: 50U/g
(4), after enzymolysis terminates, two-step fermenting is used to ferment；Detailed process is: 1) one time fermentation:
The Semen Maydis powder of the 1% of addition straw dry matter weight, adds bacillus subtilis (CICC 10088) and produces
Protein candida mycoderma (CICC 1314) each 2.5% (volume mass than), 30 degree of fermentation process 72 hours；2)
Ferment in second time: after one time fermentation terminates, the sucrose of the 1% of addition straw dry matter weight and the pH value of 1%
It is the phosphate buffer of 5.0, mixing；Being subsequently adding lactobacillus inoculum amount is 5% (volume mass ratio),
35 DEG C of fermentation process 72 hours.
(5) by the straw feed fermented directly for preserving after animal edible or drying.
After fermentation ends, the neutral detergent fiber in straw is down to 62.5% by 67.1%, and crude fibre is by 27% fall
To 22.9%, crude protein is increased to 7.79% by 6.78%, and in straw, total acid content reaches 3.19%.
From above-described embodiment it can be seen that use after corn straw processes by technical scheme,
Reduce the content of cellulose, improve the content of protein, thus be effectively improved the nutriture value of straw
Value, adds the palatability of straw simultaneously, meets the nutritional need of the animals such as cattle and sheep.
Claims (6)
1. the preparation method of a corn straw feedstuff, it is characterised in that: comprise the steps:
1) Mechanical comminution techniques is used to process corn straw, a diameter of 0.2-1mm of granule after pulverizing；
2) straw after pulverizing is put in container, and through high temperature steaming 30-60min, the straw after steaming and decocting is spread out
Cool；
3) compound enzymic preparation is dissolved in water, mixes with straw, carry out room temperature enzymolysis 5-6h；
4), after enzymolysis terminates, two-step fermenting is used to ferment；
5) by the straw feed fermented directly for pulverizing storage after animal edible or drying.
2. the preparation method of corn straw feedstuff as claimed in claim 1, it is characterised in that described machinery
Crushing technology includes using blade type pulverizer, beater grinder or ball mill pulverizer to pulverize.
3. the preparation method of corn straw feedstuff as claimed in claim 1, it is characterised in that described answers
Synthase enzyme preparation is by xylanase, cellulase and hemicellulase composition, and addition is: xylanase
200-400U/g, cellulase 50-100U/g, hemicellulase 50-100U/g.
4. the preparation method of corn straw feedstuff as claimed in claim 1, it is characterised in that described two steps
Fermentation method is: 1) one time fermentation: the Semen Maydis powder of the 1% of addition straw dry matter weight, adds hay bud
Spore bacillus and yeast, inoculum concentration by volume mass ratio calculates each addition 2.5%, 28 DEG C of-32 DEG C of fermentation process
48-192 hour；2) ferment in second time: after one time fermentation terminates, adds the 0.1%-1% of straw dry matter weight
Sucrose and the phosphate buffer of pH 4.0-5.0 of 1%, mixing, then inoculum concentration by volume mass ratio adds
Enter the lactic acid bacteria fermentation of 5%, 35-37 DEG C of fermentation process 48-96 hour.
5. the preparation method of corn straw feedstuff as claimed in claim 1, it is characterised in that described yeast
Bacterium is Candida utilis or Hansenula anomala.
6. the preparation method of corn straw feedstuff as claimed in claim 1, it is characterised in that described lactic acid
Bacterium is Lactobacillus plantarum.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610304931.XA CN105961854A (en) | 2016-05-10 | 2016-05-10 | Preparation method of corn straw feed |
Applications Claiming Priority (1)
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Cited By (20)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106509361A (en) * | 2016-11-03 | 2017-03-22 | 北海益生源农贸有限责任公司 | Livestock feed by taking banana stem and leaves as main materials |
| CN106720997A (en) * | 2016-12-07 | 2017-05-31 | 滨州市科学技术情报研究所 | A kind of enzymatic hydrolysis and fermentation cooperates with whole-plant Corn Silage new technology |
| CN106819412A (en) * | 2017-01-24 | 2017-06-13 | 兴安县金银山果蔬专业合作社 | The processing technology of agricultural crop straw |
| CN107019113A (en) * | 2017-03-22 | 2017-08-08 | 邵爱珍 | A kind of preparation method for the corn straw feedstuff that goose is combined with alfalfa meal rye grass meal |
| CN107041457A (en) * | 2017-06-14 | 2017-08-15 | 承德博亚农牧发展有限责任公司 | A kind of biological feedstuff and preparation method thereof |
| CN107048059A (en) * | 2017-03-22 | 2017-08-18 | 邵爱珍 | A kind of preparation method of broiler chicken fermentation ginkgo leaf powder maize straw composite biological feed |
| CN107048058A (en) * | 2017-03-22 | 2017-08-18 | 邵爱珍 | A kind of preparation method for the corn straw feedstuff that laying hen is combined with mulberry leaf powder Quercetin |
| CN107751615A (en) * | 2017-12-01 | 2018-03-06 | 无为县雨露生态农业有限公司 | Prevent the mixed feed of grice diarrhoea |
| CN108029862A (en) * | 2017-12-29 | 2018-05-15 | 五河县纬立农业科技有限公司 | The method that a kind of corn and maize straw integrally ferment |
| CN108029879A (en) * | 2017-12-01 | 2018-05-15 | 无为县雨露生态农业有限公司 | Weanling pig mixed feed |
| CN108142660A (en) * | 2018-02-01 | 2018-06-12 | 石家庄虎吟潭荒山开发股份有限公司 | A kind of preparation method of straw feed |
| CN108157589A (en) * | 2017-12-28 | 2018-06-15 | 河南健特生物科技集团有限公司 | One breeding class biological feedstuff and preparation method thereof |
| CN108244346A (en) * | 2017-12-28 | 2018-07-06 | 河南健特生物科技集团有限公司 | A kind of fowl poultry kind biological feed additive |
| CN108740300A (en) * | 2018-04-19 | 2018-11-06 | 河南农业大学 | A method of preparing nonruminant energy feed using agricultural crop straw |
| CN109287855A (en) * | 2018-09-28 | 2019-02-01 | 青岛农业大学 | A kind of fermentation process of corn stover, the fermented maize stalk of this method preparation and application |
| CN109805176A (en) * | 2019-02-27 | 2019-05-28 | 吉林农业大学 | A kind of preparation method of corn stover Huang storage feed |
| CN110313540A (en) * | 2019-07-11 | 2019-10-11 | 陕西省生物农业研究所 | A kind of method of composite ferment and maize straw biological Huang storage preparation forage grass |
| CN112293565A (en) * | 2020-11-11 | 2021-02-02 | 辽宁祥和农牧实业有限公司 | Formula of puffed biological straw feed |
| CN108354061B (en) * | 2018-02-28 | 2021-07-16 | 苏州昆蓝生物科技有限公司 | Production method of intestinal regulator |
| CN113796455A (en) * | 2021-09-25 | 2021-12-17 | 广西壮族自治区环境保护科学研究院 | Process for producing feed by using crop straws |
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Cited By (21)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106509361A (en) * | 2016-11-03 | 2017-03-22 | 北海益生源农贸有限责任公司 | Livestock feed by taking banana stem and leaves as main materials |
| CN106720997A (en) * | 2016-12-07 | 2017-05-31 | 滨州市科学技术情报研究所 | A kind of enzymatic hydrolysis and fermentation cooperates with whole-plant Corn Silage new technology |
| CN106819412A (en) * | 2017-01-24 | 2017-06-13 | 兴安县金银山果蔬专业合作社 | The processing technology of agricultural crop straw |
| CN107019113A (en) * | 2017-03-22 | 2017-08-08 | 邵爱珍 | A kind of preparation method for the corn straw feedstuff that goose is combined with alfalfa meal rye grass meal |
| CN107048059A (en) * | 2017-03-22 | 2017-08-18 | 邵爱珍 | A kind of preparation method of broiler chicken fermentation ginkgo leaf powder maize straw composite biological feed |
| CN107048058A (en) * | 2017-03-22 | 2017-08-18 | 邵爱珍 | A kind of preparation method for the corn straw feedstuff that laying hen is combined with mulberry leaf powder Quercetin |
| CN107041457A (en) * | 2017-06-14 | 2017-08-15 | 承德博亚农牧发展有限责任公司 | A kind of biological feedstuff and preparation method thereof |
| CN107751615A (en) * | 2017-12-01 | 2018-03-06 | 无为县雨露生态农业有限公司 | Prevent the mixed feed of grice diarrhoea |
| CN108029879A (en) * | 2017-12-01 | 2018-05-15 | 无为县雨露生态农业有限公司 | Weanling pig mixed feed |
| CN108244346A (en) * | 2017-12-28 | 2018-07-06 | 河南健特生物科技集团有限公司 | A kind of fowl poultry kind biological feed additive |
| CN108157589A (en) * | 2017-12-28 | 2018-06-15 | 河南健特生物科技集团有限公司 | One breeding class biological feedstuff and preparation method thereof |
| CN108029862A (en) * | 2017-12-29 | 2018-05-15 | 五河县纬立农业科技有限公司 | The method that a kind of corn and maize straw integrally ferment |
| CN108142660A (en) * | 2018-02-01 | 2018-06-12 | 石家庄虎吟潭荒山开发股份有限公司 | A kind of preparation method of straw feed |
| CN108354061B (en) * | 2018-02-28 | 2021-07-16 | 苏州昆蓝生物科技有限公司 | Production method of intestinal regulator |
| CN108740300A (en) * | 2018-04-19 | 2018-11-06 | 河南农业大学 | A method of preparing nonruminant energy feed using agricultural crop straw |
| CN108740300B (en) * | 2018-04-19 | 2021-12-21 | 河南农业大学 | Method for preparing monogastric animal energy feed by using crop straws |
| CN109287855A (en) * | 2018-09-28 | 2019-02-01 | 青岛农业大学 | A kind of fermentation process of corn stover, the fermented maize stalk of this method preparation and application |
| CN109805176A (en) * | 2019-02-27 | 2019-05-28 | 吉林农业大学 | A kind of preparation method of corn stover Huang storage feed |
| CN110313540A (en) * | 2019-07-11 | 2019-10-11 | 陕西省生物农业研究所 | A kind of method of composite ferment and maize straw biological Huang storage preparation forage grass |
| CN112293565A (en) * | 2020-11-11 | 2021-02-02 | 辽宁祥和农牧实业有限公司 | Formula of puffed biological straw feed |
| CN113796455A (en) * | 2021-09-25 | 2021-12-17 | 广西壮族自治区环境保护科学研究院 | Process for producing feed by using crop straws |
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