CN105961854A - Preparation method of corn straw feed - Google Patents

Preparation method of corn straw feed Download PDF

Info

Publication number
CN105961854A
CN105961854A CN201610304931.XA CN201610304931A CN105961854A CN 105961854 A CN105961854 A CN 105961854A CN 201610304931 A CN201610304931 A CN 201610304931A CN 105961854 A CN105961854 A CN 105961854A
Authority
CN
China
Prior art keywords
straw
fermentation
corn straw
feedstuff
corn
Prior art date
Application number
CN201610304931.XA
Other languages
Chinese (zh)
Inventor
赵有玺
马榴强
冀颐之
龚平
程艳玲
Original Assignee
北京联合大学
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 北京联合大学 filed Critical 北京联合大学
Priority to CN201610304931.XA priority Critical patent/CN105961854A/en
Publication of CN105961854A publication Critical patent/CN105961854A/en

Links

Classifications

    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23YINDEXING SCHEME RELATING TO LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23Y2220/00Lactobacillus
    • A23Y2220/67Plantarum
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87Re-use of by-products of food processing for fodder production

Abstract

The invention relates to a preparation method of a corn straw feed. The preparation method comprises the following steps of treating corn straw through a mechanical pulverization technique, performing high-temperature steaming on the pulverized straw so as to realize the purposes of performing sterilization and softening fibers, performing treatment through a compound enzyme preparation, and performing fermentation treatment through various microbe strains. According to the preparation method disclosed by the invention, degradation of cellulose and hemicellulose in the straw is realized through various manners, the quantity of nutrient substances, such as protein, in the feed, is increased, the nutrient value of the straw is increased, and nutritional requirements of animals such as cattle and sheep are met.

Description

A kind of preparation method of corn straw feedstuff
Technical field
The invention belongs to technique for producing feed field, be specifically related to a kind of corn stalk with better nutritivity value The preparation method of stalk feed.
Background technology
China's agricultural straw resource is the abundantest, and annual production reaches more than 800,000,000 ton, straw kind mainly with Semen Maydis, Oryza sativa L. and Semen Tritici aestivi are main, and wherein corn straw occupancy volume is maximum.Corn straw is mainly by cellulose (31%~40%), hemicellulose (35%~48%) and three kinds of material compositions of lignin (15%~25%).Crude protein contains Measuring low, vitamin deficiency, nutritive value is the highest.Therefore, corn straw has become main agricultural wastes, The discarded phenomenon of burning of China's vast rural area straw is extremely serious, and this not only causes the huge waste of resource, And add atmospheric pollution, make China's haze weather make the matter worse, seriously run counter to China's sustainable development Theory.
Meanwhile, China, due to the fast development of aquaculture, causes feedstuff shortage.The grain resource of China is very Limited, the high speed development of aquaculture, cause people and animals and strive the rise of grain and grain price, cause aquaculture simultaneously Cost remain high.Corn straw is owing to mainly containing cellulose, hemicellulose and lignin, crude protein Content is low, vitamin deficiency, thus is the roughage that a kind of nutritive value is the highest.Therefore, by modern skill Art means, improve the nutritive value of straw, have great importance.It is possible not only to realize the height of corn straw Effect utilizes, and can alleviate the feedstuff grain demand of China, and asks for solving the environment of crop straw burning Topic, has certain meaning.
At present, having employing fermentable, the method corn straw such as mechanical treatment carries out the method processed, and Achieve certain effect.But the feedstuff produced still cannot meet the nutritional need of animal, still needs to other and raises Expect with the use of.
Summary of the invention
It is an object of the invention to provide the preparation method of a kind of corn straw feedstuff, the method passes through enzymolysis and two Step fermentation can effectively improve the nutritive value of corn straw, and the corn straw feedstuff produced can directly be raised Feed animal, it is not necessary to add other nutrients again.
To achieve these goals, the technical solution used in the present invention is as follows:
The present invention provides the preparation method of a kind of corn straw feedstuff, comprises the steps:
1) Mechanical comminution techniques is used to process corn straw, a diameter of 0.2-1mm of granule after pulverizing;
2) straw after pulverizing is put in container, and through high temperature steaming 30-60min, the straw after steaming and decocting is spread out Cooling, this step is for sterilization and softening fibre;
3) compound enzymic preparation is dissolved in water, mixes with straw, carry out room temperature enzymolysis 5-6h;
4), after enzymolysis terminates, two-step fermenting is used to ferment;
5) by the straw feed fermented directly for pulverizing storage after animal edible or drying.
Preferably, described Mechanical comminution techniques includes using blade type pulverizer, beater grinder or ball milling powder Broken machine is pulverized.
Preferably, described compound enzyme enzyme preparation is made up of xylanase, cellulase and hemicellulase, Addition is: xylanase 200-400U/g, cellulase 50-100U/g and hemicellulase 50-100 U/g。
Preferably, described two-step fermenting is: 1) one time fermentation: the 1% of addition straw dry matter weight Semen Maydis powder, adds bacillus subtilis and yeast, and inoculum concentration by volume mass ratio calculates each addition 2.5%, 28 DEG C of-32 DEG C of fermentation process 48-192 hour;2) ferment in second time: after one time fermentation terminates, adds straw and does The sucrose of the 0.1%-1% of substance weight and the phosphate buffer of the pH 4.0-5.0 of 1%, mixing, then connect Plant and measure the lactic acid bacteria fermentation that by volume mass ratio adds 5%, 35-37 DEG C of fermentation process 48-96 hour.
Preferably, described yeast is Candida utilis or Hansenula anomala.
Preferably, described lactic acid bacteria is Lactobacillus plantarum.
Run into cold snap during enzymolysis, can use and heat or prolong long mode, if ambient temperature fall Low to 15-20 DEG C, 24h can be extended to, if temperature is less than 15 DEG C, then be difficult to enzymolysis, need to take Measure improves ambient temperature.
Present invention is characterized in that
Using xylanase in the present invention, cellulase, hemicellulase can be with the fibre in degrading maize straws Dimension element, hemicellulose and lignin, obtain the small-molecular-weight sugar that animal can effectively utilize.
Add Semen Maydis powder when one time fermentation and primarily serve the purpose of withered bacillus cereus and yeast extra-nutrition material, Hay spore can produce multiple enzyme, can polymer substance in degrading straw further;Adding yeast can To utilize the small-molecule substance of generation of being degraded by enzymolysis and bacillus subtilis, further synthesising thalli protein, Improve the protein content of straw.
Adding sucrose when ferment in second time and be chiefly to facilitate the growth of lactic acid bacteria, lactic acid bacteria is in growth and breeding process In available sugar produce organic acid (lactic acid), make organic acid content raise, the mouthfeel of feedstuff, straw can be improved Palatability be obviously improved, straw feed conversion ratio improves, and organic acid can substitute for feeding antibiotic and press down Harmful microbe processed grows, and improves the body immunity of domestic animal, prevents and treat intestinal tract disease.
Complex enzyme zymohydrolysis is separated with fermentable by the present invention, is owing to enzymolysis is different from fermentation condition, If carried out, it is impossible to obtain optimum efficiency simultaneously.Particularly fermentation stage, also especially by bacillus subtilis, Yeast separately ferments with lactic acid bacteria, is owing to lactic acid bacteria fermentation produces acid, can cause suppressing hay spore Bacillus and saccharomycetic growth, cause fermenting unsuccessfully.After Fen Kai, be conducive to the pH etc. by regulating straw Method, makes rapid microbial growth.
Strain used by the present invention, all buys from Chinese industrial Microbiological Culture Collection administrative center (CICC). Wherein bacillus subtilis, numbered CICC 10088;Candida utilis, numbered CICC 1314; Hansenula anomala, numbered CICC 32257;Lactobacillus plantarum, numbered CICC 21794.
The beneficial effects of the present invention is:
The present invention provides the preparation method of a kind of corn straw feedstuff, and the method uses enzymolysis and ferment in second time In conjunction with processing, it is effectively improved the content of the nutrient substance such as protein in corn straw, thus improves jade The nutritive value of rice straw, it is not necessary to add other nutrients again, and palatability is strong, meet cattle and sheep etc. and move The nutritional need of thing.
Detailed description of the invention
Technical scheme is further illustrated below by detailed description of the invention.Described embodiment is only It is to aid in understanding the present invention, is not construed as the concrete restriction to the present invention.
Xylanase used by the present invention, cellulase and hemicellulase are all bought to be had from DSM (Chinese) Limit company.
Strain used by the present invention, all buys from Chinese industrial Microbiological Culture Collection administrative center (CICC). Wherein bacillus subtilis, numbered CICC 10088;Candida utilis, numbered CICC 1314; Hansenula anomala, numbered CICC 32257;Lactobacillus plantarum, numbered CICC 21794.
The condition of culture of microorganism used therefor is:
Bacillus subtilis culture medium prescription: peptone 5.0g, beef leaching thing 3.0g, NaCl 5.0g, steam Distilled water 1.0L, pH7.0.Condition of culture, 200 revs/min, cultivates bacterium to OD by 37 DEG C600To 1.0.
Yeast (Candida utilis or Hansenula anomala) culture medium prescription: peptone 20g, yeast Powder 10g, glucose 10g, distilled water 1.0L, pH7.0, condition of culture, 30 DEG C, 200 revs/min, will Bacterium is cultivated to OD600To 1.0.
Lactobacillus plantarum culture medium: cheese peptone 10.0g, beef powder 8.0g, yeast powder 4.0g, glucose 20.0g, Magnesium sulfate 0.2g, sodium acetate 5.0g, Triammonium citrate 2.0g, dipotassium hydrogen phosphate 2.0g, manganese sulfate 0.05g, Tween 80 1.0g, distilled water 1L, pH6.2 ± 0.2.Condition of culture, 200 revs/min, trains bacterium by 37 DEG C Support to OD600 to 1.0.
" volume mass ratio " of the present invention is L/kg, and such as, adding volume mass than the bacterium solution being 5% is 0.05L joins in 1kg, or equal proportion change.
Embodiment 1:
(1) using the mode of ball mill pulverizing to process corn straw, after pulverizing, the diameter of granule is at 0.2mm.
(2) straw after pulverizing is put in container, and through high temperature steaming 45min, the straw after steaming and decocting is spread out Cool.
(3) compound enzymic preparation is dissolved in water, mixes with straw, carry out room temperature enzymolysis 5h.
The consumption of water with straw hold agglomerating, unclamp and i.e. dissipate for most preferably.
Compound enzymic preparation includes: xylanase, cellulase and hemicellulase, and consumption is:
The addition of enzyme preparation is:
Xylanase: 400U/g;
Cellulase: 50U/g;
Hemicellulase: 50U/g;
(4), after enzymolysis terminates, two-step fermenting is used to ferment;Detailed process is: 1) one time fermentation: The Semen Maydis powder of the 1% of addition straw dry matter weight, adds bacillus subtilis (CICC 10088) and produces Each 2.5% (the volume mass ratio) of protein candida mycoderma (CICC 1314), 30 degree, fermentation process 48 hours; 2) ferment in second time: after one time fermentation terminates, add straw dry matter weight 0.5% sucrose and 1% The phosphate buffer of pH5.0, mixing;It is subsequently adding Lactobacillus plantarum (CICC 21794) fermentation inoculation Amount be 5% (volume mass than), 37 DEG C of fermentation process 48 hours.
(5) by the straw feed fermented directly for preserving after animal edible or drying.
After fermentation ends, the neutral detergent fiber in measuring and calculating straw is down to 62.3% by 67.1%, and crude fibre is by 27% Being down to 21.4%, crude protein is increased to 8.32% by 6.78%, and in straw, total acid content reaches 2.91%.
Embodiment 2:
(1) using the mode of hammer type crushing to process corn straw, after pulverizing, the diameter of granule is at 0.5mm.
(2) straw after pulverizing is put in container, high temperature steaming 35min), the straw after steaming and decocting is spread out Cool.
(3) compound enzymic preparation is dissolved in water, mixes with straw, carry out enzymolysis 5h.
The consumption of water with straw hold agglomerating, unclamp and i.e. dissipate for most preferably.
Compound enzymic preparation includes: xylanase, cellulase and hemicellulase, and consumption is:
The addition of enzyme preparation is:
Xylanase: 200U/g
Cellulase: 100U/g
Hemicellulase: 100U/g
(4), after enzymolysis terminates, two-step fermenting is used to ferment;Detailed process is: 1) one time fermentation: Add straw dry matter weight 1% Semen Maydis powder, add bacillus subtilis (CICC 10088) and different Often Hansenula yeast (CICC 32257) each 2.5% (volume mass than), 30 degree of fermentation process 96 hours;2) Ferment in second time: after one time fermentation terminates, the sucrose of the 1% of addition straw dry matter weight and the pH value of 1% It is the phosphate buffer of 4.5, mixing;It is subsequently adding Lactobacillus plantarum (CICC 21794), sends out for 35 DEG C Ferment processes 96 hours.
(5) by the straw feed fermented directly for preserving after animal edible or drying.
After fermentation ends, the neutral detergent fiber in straw is down to 63.1% by 67.1%, and crude fibre is by 27% fall To 20.9%, crude protein is increased to 7.92% by 6.78%, and in straw, total acid content reaches 2.79%.
Embodiment 3:
(1) mode using blade type to pulverize processes corn straw, a diameter of 1mm of granule after pulverizing.
(2) straw after pulverizing is put in container, and high temperature steaming 30min, the straw after steaming and decocting is spread out and put Cool.
(3) being dissolved in water by compound enzymic preparation, mix with straw, room temperature carries out enzymolysis 6h.
The consumption of water with straw hold agglomerating, unclamp and i.e. dissipate for most preferably.
Compound enzymic preparation includes: xylanase, cellulase and hemicellulase, and consumption is:
Xylanase: 300U/g
Cellulase: 80U/g
Hemicellulase: 50U/g
(4), after enzymolysis terminates, two-step fermenting is used to ferment;Detailed process is: 1) one time fermentation: The Semen Maydis powder of the 1% of addition straw dry matter weight, adds bacillus subtilis (CICC 10088) and produces Protein candida mycoderma (CICC 1314) each 2.5% (volume mass than), 30 degree of fermentation process 72 hours;2) Ferment in second time: after one time fermentation terminates, the sucrose of the 1% of addition straw dry matter weight and the pH value of 1% It is the phosphate buffer of 5.0, mixing;Being subsequently adding lactobacillus inoculum amount is 5% (volume mass ratio), 35 DEG C of fermentation process 72 hours.
(5) by the straw feed fermented directly for preserving after animal edible or drying.
After fermentation ends, the neutral detergent fiber in straw is down to 62.5% by 67.1%, and crude fibre is by 27% fall To 22.9%, crude protein is increased to 7.79% by 6.78%, and in straw, total acid content reaches 3.19%.
From above-described embodiment it can be seen that use after corn straw processes by technical scheme, Reduce the content of cellulose, improve the content of protein, thus be effectively improved the nutriture value of straw Value, adds the palatability of straw simultaneously, meets the nutritional need of the animals such as cattle and sheep.

Claims (6)

1. the preparation method of a corn straw feedstuff, it is characterised in that: comprise the steps:
1) Mechanical comminution techniques is used to process corn straw, a diameter of 0.2-1mm of granule after pulverizing;
2) straw after pulverizing is put in container, and through high temperature steaming 30-60min, the straw after steaming and decocting is spread out Cool;
3) compound enzymic preparation is dissolved in water, mixes with straw, carry out room temperature enzymolysis 5-6h;
4), after enzymolysis terminates, two-step fermenting is used to ferment;
5) by the straw feed fermented directly for pulverizing storage after animal edible or drying.
2. the preparation method of corn straw feedstuff as claimed in claim 1, it is characterised in that described machinery Crushing technology includes using blade type pulverizer, beater grinder or ball mill pulverizer to pulverize.
3. the preparation method of corn straw feedstuff as claimed in claim 1, it is characterised in that described answers Synthase enzyme preparation is by xylanase, cellulase and hemicellulase composition, and addition is: xylanase 200-400U/g, cellulase 50-100U/g, hemicellulase 50-100U/g.
4. the preparation method of corn straw feedstuff as claimed in claim 1, it is characterised in that described two steps Fermentation method is: 1) one time fermentation: the Semen Maydis powder of the 1% of addition straw dry matter weight, adds hay bud Spore bacillus and yeast, inoculum concentration by volume mass ratio calculates each addition 2.5%, 28 DEG C of-32 DEG C of fermentation process 48-192 hour;2) ferment in second time: after one time fermentation terminates, adds the 0.1%-1% of straw dry matter weight Sucrose and the phosphate buffer of pH 4.0-5.0 of 1%, mixing, then inoculum concentration by volume mass ratio adds Enter the lactic acid bacteria fermentation of 5%, 35-37 DEG C of fermentation process 48-96 hour.
5. the preparation method of corn straw feedstuff as claimed in claim 1, it is characterised in that described yeast Bacterium is Candida utilis or Hansenula anomala.
6. the preparation method of corn straw feedstuff as claimed in claim 1, it is characterised in that described lactic acid Bacterium is Lactobacillus plantarum.
CN201610304931.XA 2016-05-10 2016-05-10 Preparation method of corn straw feed CN105961854A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201610304931.XA CN105961854A (en) 2016-05-10 2016-05-10 Preparation method of corn straw feed

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201610304931.XA CN105961854A (en) 2016-05-10 2016-05-10 Preparation method of corn straw feed

Publications (1)

Publication Number Publication Date
CN105961854A true CN105961854A (en) 2016-09-28

Family

ID=56991511

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201610304931.XA CN105961854A (en) 2016-05-10 2016-05-10 Preparation method of corn straw feed

Country Status (1)

Country Link
CN (1) CN105961854A (en)

Cited By (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106509361A (en) * 2016-11-03 2017-03-22 北海益生源农贸有限责任公司 Livestock feed by taking banana stem and leaves as main materials
CN106720997A (en) * 2016-12-07 2017-05-31 滨州市科学技术情报研究所 A kind of enzymatic hydrolysis and fermentation cooperates with whole-plant Corn Silage new technology
CN106819412A (en) * 2017-01-24 2017-06-13 兴安县金银山果蔬专业合作社 The processing technology of agricultural crop straw
CN107019113A (en) * 2017-03-22 2017-08-08 邵爱珍 A kind of preparation method for the corn straw feedstuff that goose is combined with alfalfa meal rye grass meal
CN107041457A (en) * 2017-06-14 2017-08-15 承德博亚农牧发展有限责任公司 A kind of biological feedstuff and preparation method thereof
CN107048059A (en) * 2017-03-22 2017-08-18 邵爱珍 A kind of preparation method of broiler chicken fermentation ginkgo leaf powder maize straw composite biological feed
CN107048058A (en) * 2017-03-22 2017-08-18 邵爱珍 A kind of preparation method for the corn straw feedstuff that laying hen is combined with mulberry leaf powder Quercetin
CN107751615A (en) * 2017-12-01 2018-03-06 无为县雨露生态农业有限公司 Prevent the mixed feed of grice diarrhoea
CN108029862A (en) * 2017-12-29 2018-05-15 五河县纬立农业科技有限公司 The method that a kind of corn and maize straw integrally ferment
CN108029879A (en) * 2017-12-01 2018-05-15 无为县雨露生态农业有限公司 Weanling pig mixed feed
CN108142660A (en) * 2018-02-01 2018-06-12 石家庄虎吟潭荒山开发股份有限公司 A kind of preparation method of straw feed
CN108157589A (en) * 2017-12-28 2018-06-15 河南健特生物科技集团有限公司 One breeding class biological feedstuff and preparation method thereof
CN108244346A (en) * 2017-12-28 2018-07-06 河南健特生物科技集团有限公司 A kind of fowl poultry kind biological feed additive

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1556213A (en) * 2004-01-02 2004-12-22 曹全星 Method of manufacturing alcohol and blending feedstuff using corn straw
CN102783561A (en) * 2012-07-30 2012-11-21 江南大学 Preparation method for energy-saving biological straw feed
CN103564157A (en) * 2012-07-31 2014-02-12 梁翠 Processing method of crop straw feed
CN104256057A (en) * 2014-10-01 2015-01-07 青岛嘉瑞生物技术有限公司 Method for preparing feed proteins by utilizing alcohol waste liquor and crop straws

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1556213A (en) * 2004-01-02 2004-12-22 曹全星 Method of manufacturing alcohol and blending feedstuff using corn straw
CN102783561A (en) * 2012-07-30 2012-11-21 江南大学 Preparation method for energy-saving biological straw feed
CN103564157A (en) * 2012-07-31 2014-02-12 梁翠 Processing method of crop straw feed
CN104256057A (en) * 2014-10-01 2015-01-07 青岛嘉瑞生物技术有限公司 Method for preparing feed proteins by utilizing alcohol waste liquor and crop straws

Cited By (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106509361A (en) * 2016-11-03 2017-03-22 北海益生源农贸有限责任公司 Livestock feed by taking banana stem and leaves as main materials
CN106720997A (en) * 2016-12-07 2017-05-31 滨州市科学技术情报研究所 A kind of enzymatic hydrolysis and fermentation cooperates with whole-plant Corn Silage new technology
CN106819412A (en) * 2017-01-24 2017-06-13 兴安县金银山果蔬专业合作社 The processing technology of agricultural crop straw
CN107048059A (en) * 2017-03-22 2017-08-18 邵爱珍 A kind of preparation method of broiler chicken fermentation ginkgo leaf powder maize straw composite biological feed
CN107019113A (en) * 2017-03-22 2017-08-08 邵爱珍 A kind of preparation method for the corn straw feedstuff that goose is combined with alfalfa meal rye grass meal
CN107048058A (en) * 2017-03-22 2017-08-18 邵爱珍 A kind of preparation method for the corn straw feedstuff that laying hen is combined with mulberry leaf powder Quercetin
CN107041457A (en) * 2017-06-14 2017-08-15 承德博亚农牧发展有限责任公司 A kind of biological feedstuff and preparation method thereof
CN107751615A (en) * 2017-12-01 2018-03-06 无为县雨露生态农业有限公司 Prevent the mixed feed of grice diarrhoea
CN108029879A (en) * 2017-12-01 2018-05-15 无为县雨露生态农业有限公司 Weanling pig mixed feed
CN108157589A (en) * 2017-12-28 2018-06-15 河南健特生物科技集团有限公司 One breeding class biological feedstuff and preparation method thereof
CN108244346A (en) * 2017-12-28 2018-07-06 河南健特生物科技集团有限公司 A kind of fowl poultry kind biological feed additive
CN108029862A (en) * 2017-12-29 2018-05-15 五河县纬立农业科技有限公司 The method that a kind of corn and maize straw integrally ferment
CN108142660A (en) * 2018-02-01 2018-06-12 石家庄虎吟潭荒山开发股份有限公司 A kind of preparation method of straw feed

Similar Documents

Publication Publication Date Title
Sadh et al. Agro-industrial wastes and their utilization using solid state fermentation: a review
CN102550804B (en) The preparation method of the forage protein of a kind of leftovers of tilapia and dregs of beans mixed culture fermentation
CN102972635B (en) Method for producing microorganism feed additive by utilizing probiotics and mixed meal
CN101531985B (en) High-efficiency ferment for fermenting bean pulp and bean pulp fermentation technology using the ferment
CN102613329B (en) Preparation method of raw tea enzyme and raw tea enzyme
CN104541972A (en) Method for cultivating edible fungi through agricultural straws
CN105685486A (en) Preparation method of natural high-fiber odorless cattle and sheep feed
CN101248845B (en) Method for processing raw material of aquatic product feed stuff by fermenting
CN103922818B (en) Utilize sugar mill mud to produce technology and the technique of biological humic acid
Ezekiel et al. Protein enrichment of cassava peel by submerged fermentation with Trichoderma viride (ATCC 36316)
CN103004659B (en) Fish fry culturing method for four major Chinese carps
CN104256057A (en) Method for preparing feed proteins by utilizing alcohol waste liquor and crop straws
CN101390566B (en) Manifold microbe mixed culture fermentation agent and method for producing high energy protein biology feedstuff
CN104996722B (en) A kind of method of the step combined ferment feed of multi-cultur es two
CN103749967B (en) Sweet potato dregs fermented feed and preparation method thereof
Yang et al. Bioconversion of corn straw by coupling ensiling and solid-state fermentation
CN101715872B (en) Method for preparing high-protein sacchariferous feed by straws
CN101412648A (en) Polyzyme microbial growth-promoting additive for plants
CN102715350A (en) Production method of straw feed
CN106173363A (en) The method producing cattle and sheep complete feed for raw material ferment in second time with edible fungus bran
CN102178033B (en) Combined bacteria fermented bran high protein predigesting feed and preparation method
CN102599351B (en) Bio-enzyme and microorganism containing compound feed additive and preparation process thereof
CN102864095B (en) Lactobacillus plantarum used for silage alfalfa and use method thereof
CN102077903A (en) Method for jointly treating stalks by steam explosion and microorganism fermentation
CN102505003B (en) High-density fermenting method of bacillus coagulans for livestock and poultry, as well as preparation prepared by method and application thereof

Legal Events

Date Code Title Description
PB01 Publication
C06 Publication
SE01 Entry into force of request for substantive examination
C10 Entry into substantive examination
RJ01 Rejection of invention patent application after publication

Application publication date: 20160928

RJ01 Rejection of invention patent application after publication