CN107048059A - A kind of preparation method of broiler chicken fermentation ginkgo leaf powder maize straw composite biological feed - Google Patents
A kind of preparation method of broiler chicken fermentation ginkgo leaf powder maize straw composite biological feed Download PDFInfo
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- CN107048059A CN107048059A CN201710173315.XA CN201710173315A CN107048059A CN 107048059 A CN107048059 A CN 107048059A CN 201710173315 A CN201710173315 A CN 201710173315A CN 107048059 A CN107048059 A CN 107048059A
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- maize straw
- ginkgo leaf
- leaf powder
- fermentation
- broiler chicken
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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Abstract
The invention discloses a kind of broiler chicken fermentation ginkgo leaf powder maize straw composite biological feed, and its preparation technology, characterized in that, with ginkgo leaf powder, maize straw, ammonium sulfate, dusty yeast, production flesh Candida, peptone, NaCl, bacillus licheniformis, zytase, glucose solution, citrate buffer solution etc. for raw material.The present invention destroys the cell wall structure of maize straw by the preprocess method of thermophilic digestion, hence it is evident that improve the hydrolysis result of maize straw；Immersion treatment is carried out to it with dilute NaOH solution again, maize straw constituent content and crystal structure is changed, is conducive to follow-up proteolysis assay.Stalk is converted using zytase, reduced sugar, cellulose, hemicellulose etc. are obtained after conversion, using the method for first digesting after fermentation, protein content is improved.Ginkgo leaf powder fermentate is added in broiler fodder, the feed efficiency of broiler chicken can be improved, improves the tenderness and anti-oxidation function of broiler chicken.
Description
Technical field
The present invention relates to field of fodder, and in particular to a kind of broiler chicken is raised with fermentation ginkgo leaf powder-maize straw compound bio
The preparation method of material.
Background technology
China's agricultural crop straw yield is high, most of not developed reasonably, and serious waste of resources is directly burned
Serious pollution is caused to environment, it is carried out after processing, feed can be prepared, obtains rationally sufficiently using, delays
Energy crisis and problem of environmental pollution are solved.
Zhang Xiaoqian is in its master thesis《A kind of preparation of novel straw biological feedstuff》In one text, using different sides
Method carries out straw feed pretreatment, as a result shows that the effect handled during with 1% dilute sodium hydroxide, 120 DEG C is best；To pre- place
Stalk after reason carries out proteolysis assay, and stalk is converted using cellulase and zytase complex enzyme, it is determined that optimal enzyme
Solution condition is temperature 50 C, pH 7, enzyme addition 25U/g, solid-to-liquid ratio 1:10, shaking speed 140rpm, enzymolysis efficiency is optimal, this
When reduction candy output be 0.33g (1g stalks).But there is the problem of conversion ratio is low, nutrition is single.
The present invention destroys the cell wall structure of maize straw by the preprocess method of thermophilic digestion, hence it is evident that improve jade
The hydrolysis result of rice stalk；Immersion treatment is carried out to it with dilute NaOH solution again, maize straw constituent content and crystal is changed
Structure, is conducive to follow-up proteolysis assay.Stalk is converted using zytase, reduced sugar, cellulose, half are obtained after conversion
Cellulose etc., using the method for first digesting after fermentation, improves protein content, is prepared for composite stalk feed.Ginkgo leaf is rich in
The various active material such as amino acid, polysaccharide, trace element, have influence on the metabolism of animal body, with growth promotion, disease resistance
Effect, using ginkgo leaf powder as fermentation substrate, using bacillus licheniformis as fermented bacterium, is fermented；Added in broiler fodder
Ginkgo leaf powder fermentate, can improve the feed efficiency of broiler chicken, improve the tenderness and anti-oxidation function of broiler chicken.
The content of the invention
The present invention solves the technical problem of provide a kind of broiler chicken fermentation ginkgo leaf powder-maize straw compound bio
The preparation method of feed, the forage protein content prepared according to the technique is high, can improve the feed efficiency, tenderness and antioxygen of broiler chicken
Change function.
The technical problems to be solved by the invention are realized using following technical scheme：
Ginkgo leaf powder 20-30, maize straw 25-35, ammonium sulfate 3-6, dusty yeast 2-5, production flesh Candida 6-10, peptone 4-
8, NaCl 2-3, bacillus licheniformis 5-9, zytase, NaOH solution, glucose solution, citrate buffer solution, distilled water are fitted
Amount.
A kind of preparation method of broiler chicken fermentation ginkgo leaf powder-maize straw composite biological feed, it is characterised in that by with
Lower step is carried out：
A. maize straw is cut off, crushes, cross after 40-100 mesh sieves, at 170-190 DEG C in input pulverizer
Boiling 1-2h in thermophilic digestion tank, then by solid-to-liquid ratio 1:10-15 adds NaOH solution, in immersion treatment 1- at 90-130 DEG C
After 2h, with water washing is distilled 3-5 times, gained sample is dried at 60-80 DEG C, stand-by；
B. by after resulting material in a and ammonium sulfate, dusty yeast mixing and ball milling 1-2h, 1:15-20 is added to the lemon that pH is 4-5
In acid buffer, in 120-130 DEG C of sterilization treatment 20-40min, 1 after cooling:10-15 adds zytase, adjust pH for it is neutral,
At 50-60 DEG C after enzyme digestion reaction 10-15h, the 5-10h that fermented under production flesh Candida, normal temperature is added, degrading maize straws are obtained；
C. into peptone 1:5:20-30 adds NaCl, distilled water, stirs, and is warming up to 120-130 DEG C of sterilizing 20-
After 50min, shaken cultivation 1-2d at bacillus licheniformis, 30-40 DEG C is added；
D. ginkgo leaf powder is ground, crossed after 60-80 mesh sieves, added glucose solution, stir, 120-130 DEG C of sterilizing
1-2h, is added after resulting material in c, fermentation time 1-3d, after being mixed with resulting material in b, and appropriate distilled water, feeding are shone in spray
Grain fodder machine is conditioned, granulation, and pellet is made in re-dry, cooling.
Wherein, the concentration of NaOH solution is 1-2% in step a；Glucose concentration is 1-3% in step d, when moisture adds
When dosage is 15-25%, corn stalk pelletses feed ratio of briquetting is preferable.
The reaction mechanism of the present invention is as follows：
（1）The cell wall structure of maize straw is destroyed by the preprocess method of thermophilic digestion, hence it is evident that improve maize straw
Hydrolysis result；Immersion treatment is carried out to it with dilute NaOH solution again, the constituent content and crystal structure of maize straw is changed,
Be conducive to the progress of follow-up proteolysis assay.
（2）Stalk is converted using zytase, reduced sugar, cellulose, hemicellulose etc. are obtained after conversion, using elder generation
The method for digesting after fermentation, improves protein content, is prepared for composite stalk feed.
（3）Ginkgo leaf has influence on the metabolism of animal body rich in various active materials such as amino acid, polysaccharide, trace elements,
Effect with growth promotion, disease resistance, using ginkgo leaf powder as fermentation substrate, using bacillus licheniformis as fermented bacterium, is carried out
Fermentation；Ginkgo leaf powder fermentate is added in broiler fodder, the feed efficiency of broiler chicken can be improved, improves the tenderness and antioxygen of broiler chicken
Change function.
Embodiment
In order that the technical means, the inventive features, the objects and the advantages of the present invention are easy to understand, tie below
Specific embodiment is closed, the present invention is expanded on further.
Embodiment
A kind of broiler chicken fermentation ginkgo leaf powder-maize straw composite biological feed, by the raw material system of following weight parts (g)
：
Ginkgo leaf powder 30, maize straw 35, ammonium sulfate 6, dusty yeast 5, production flesh Candida 10, peptone 8, NaCl 3, lichens
Bacillus 9, zytase, NaOH solution, glucose solution, citrate buffer solution, distillation appropriate amount of water.
A kind of preparation method of broiler chicken fermentation ginkgo leaf powder-maize straw composite biological feed, it is characterised in that by with
Lower step is carried out：
A. maize straw is cut off, crushes, crossed after 60 mesh sieves in input pulverizer, steamed in 170-180 DEG C of thermophilic digestion tank
2h is boiled, then by solid-to-liquid ratio 1:15 add NaOH solutions, in after immersion treatment 2h at 110-120 DEG C, with distillation water washing 3 times, institute
Sample is obtained to dry at 60-70 DEG C, it is stand-by；
B. by after resulting material in a and ammonium sulfate, dusty yeast mixing and ball milling 1h, 1:20 are added to the lemon acid buffering that pH is 4-5
In liquid, in 120-130 DEG C of sterilization treatment 30min, 1 after cooling:15 add zytase, and it is the neutral, enzyme at 50-60 DEG C to adjust pH
After solution reaction 10h, the 10h that fermented under production flesh Candida, normal temperature is added, degrading maize straws are obtained；
C. into peptone 1:5:30 add NaCl, distilled water, stir, and are warming up to after 120-130 DEG C of sterilizing 30min, plus
Enter shaken cultivation 1d at bacillus licheniformis, 30-40 DEG C；
D. ginkgo leaf powder is ground, crossed after 60-80 mesh sieves, added glucose solution, stir, 120-130 DEG C of sterilizing
1h, is added after resulting material in c, fermentation time 2d, after being mixed with resulting material in b, and appropriate distilled water is shone in spray, and feeding particle is raised
Material machine is conditioned, granulation, and pellet is made in re-dry, cooling.
Claims (5)
1. a kind of broiler chicken fermentation ginkgo leaf powder-maize straw composite biological feed, it is characterised in that：
After corn straw smashing, sieving, thermophilic digestion, then NaOH solution immersion treatment is used, wash, do
Dry rear and ammonium sulfate, dusty yeast mixing and ball milling, sterilization treatment, enzymatic hydrolysis and fermentation obtain degrading maize straws；By ginkgo leaf powder
Grind, sieve, add glucose solution, bacillus licheniformis, mixed after fermentation with degrading maize straws, granulation is obtained
Pellet.
2. a kind of broiler chicken preparation method of fermentation ginkgo leaf powder-maize straw composite biological feed, its feature exists
In being made by the raw material of following weight parts：
Ginkgo leaf powder 20-30, maize straw 25-35, ammonium sulfate 3-6, dusty yeast 2-5, production flesh Candida 6-10, peptone 4-
8, NaCl 2-3, bacillus licheniformis 5-9, zytase, NaOH solution, glucose solution, citrate buffer solution, distilled water are fitted
Amount.
3. a kind of broiler chicken according to claim 2 is raised with fermentation ginkgo leaf powder-maize straw compound bio
Preparation method, it is characterised in that carry out according to the following steps：
Maize straw is cut off, crushes, crosses after 40-100 mesh sieves, at 170-190 DEG C in input pulverizer
Boiling 1-2h in thermophilic digestion tank, then by solid-to-liquid ratio 1:10-15 adds NaOH solution, in immersion treatment 1- at 90-130 DEG C
After 2h, with water washing is distilled 3-5 times, gained sample is dried at 60-80 DEG C, stand-by；
B. by after resulting material in a and ammonium sulfate, dusty yeast mixing and ball milling 1-2h, 1:15-20 is added to the lemon that pH is 4-5
In acid buffer, in 120-130 DEG C of sterilization treatment 20-40min, 1 after cooling:10-15 adds zytase, adjust pH for it is neutral,
At 50-60 DEG C after enzyme digestion reaction 10-15h, the 5-10h that fermented under production flesh Candida, normal temperature is added, degrading maize straws are obtained；
C. into peptone 1:5:20-30 adds NaCl, distilled water, stirs, and is warming up to 120-130 DEG C of sterilizing 20-
After 50min, shaken cultivation 1-2d at bacillus licheniformis, 30-40 DEG C is added；
D. ginkgo leaf powder is ground, crossed after 60-80 mesh sieves, added glucose solution, stir, 120-130 DEG C of sterilizing
1-2h, is added after resulting material in c, fermentation time 1-3d, after being mixed with resulting material in b, and appropriate distilled water, feeding are shone in spray
Grain fodder machine is conditioned, granulation, and pellet is made in re-dry, cooling.
4. a kind of preparation side of broiler chicken fermentation ginkgo leaf powder-maize straw composite biological feed according to claim 3
Method, it is characterised in that the concentration of NaOH solution is 1-2% in step a.
5. a kind of preparation side of broiler chicken fermentation ginkgo leaf powder-maize straw composite biological feed according to claim 3
Method, it is characterised in that glucose concentration is 1-3% in step d；When moisture addition is 15-25%, corn stalk pelletses
Feed ratio of briquetting is preferable.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109090361A (en) * | 2018-08-24 | 2018-12-28 | 寿县昌源生猪养殖有限责任公司 | A kind of processing method of high conversion pannage |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103005159A (en) * | 2012-12-18 | 2013-04-03 | 南京林业大学 | Preparation method of ginkgo leaf biological feed additive |
| CN103564157A (en) * | 2012-07-31 | 2014-02-12 | 梁翠 | Processing method of crop straw feed |
| CN103704501A (en) * | 2013-12-09 | 2014-04-09 | 平顶山中宏华龙饲料有限公司 | Method for producing high-efficiency broiler feed by utilization of corn straw fermentation |
| CN105961854A (en) * | 2016-05-10 | 2016-09-28 | 北京联合大学 | Preparation method of corn straw feed |
-
2017
- 2017-03-22 CN CN201710173315.XA patent/CN107048059A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103564157A (en) * | 2012-07-31 | 2014-02-12 | 梁翠 | Processing method of crop straw feed |
| CN103005159A (en) * | 2012-12-18 | 2013-04-03 | 南京林业大学 | Preparation method of ginkgo leaf biological feed additive |
| CN103704501A (en) * | 2013-12-09 | 2014-04-09 | 平顶山中宏华龙饲料有限公司 | Method for producing high-efficiency broiler feed by utilization of corn straw fermentation |
| CN105961854A (en) * | 2016-05-10 | 2016-09-28 | 北京联合大学 | Preparation method of corn straw feed |
Non-Patent Citations (1)
| Title |
|---|
| 张晓谦: "一种新型秸秆生物饲料的制备", 《中国优秀硕士学位论文全文数据库 农业科技辑》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109090361A (en) * | 2018-08-24 | 2018-12-28 | 寿县昌源生猪养殖有限责任公司 | A kind of processing method of high conversion pannage |
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