CN107744148A - A kind of method of modifying of little rice bran water-soluble dietary fiber - Google Patents
A kind of method of modifying of little rice bran water-soluble dietary fiber Download PDFInfo
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- CN107744148A CN107744148A CN201710988327.8A CN201710988327A CN107744148A CN 107744148 A CN107744148 A CN 107744148A CN 201710988327 A CN201710988327 A CN 201710988327A CN 107744148 A CN107744148 A CN 107744148A
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- 238000000034 method Methods 0.000 title claims abstract description 32
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- 238000004880 explosion Methods 0.000 claims abstract description 64
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- 238000006243 chemical reaction Methods 0.000 claims abstract description 11
- 238000005119 centrifugation Methods 0.000 claims abstract description 9
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- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims description 16
- 238000000605 extraction Methods 0.000 claims description 16
- 238000001035 drying Methods 0.000 claims description 8
- AZHSSKPUVBVXLK-UHFFFAOYSA-N ethane-1,1-diol Chemical compound CC(O)O AZHSSKPUVBVXLK-UHFFFAOYSA-N 0.000 claims description 8
- 239000003208 petroleum Substances 0.000 claims description 8
- 230000002195 synergetic effect Effects 0.000 claims description 8
- 244000062793 Sorghum vulgare Species 0.000 claims description 7
- 235000019713 millet Nutrition 0.000 claims description 7
- 239000004382 Amylase Substances 0.000 claims description 6
- 102000013142 Amylases Human genes 0.000 claims description 6
- 108010065511 Amylases Proteins 0.000 claims description 6
- 108010073178 Glucan 1,4-alpha-Glucosidase Proteins 0.000 claims description 6
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- 101000693530 Staphylococcus aureus Staphylokinase Proteins 0.000 claims description 2
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- 238000006047 enzymatic hydrolysis reaction Methods 0.000 claims description 2
- 238000002203 pretreatment Methods 0.000 claims 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 abstract description 7
- 230000002255 enzymatic effect Effects 0.000 abstract 1
- 239000007789 gas Substances 0.000 description 47
- 239000000243 solution Substances 0.000 description 8
- 239000000835 fiber Substances 0.000 description 5
- QCVGEOXPDFCNHA-UHFFFAOYSA-N 5,5-dimethyl-2,4-dioxo-1,3-oxazolidine-3-carboxamide Chemical compound CC1(C)OC(=O)N(C(N)=O)C1=O QCVGEOXPDFCNHA-UHFFFAOYSA-N 0.000 description 4
- 102000002322 Egg Proteins Human genes 0.000 description 4
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- 235000005911 diet Nutrition 0.000 description 3
- 230000000378 dietary effect Effects 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
- 238000012986 modification Methods 0.000 description 3
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- 208000017667 Chronic Disease Diseases 0.000 description 2
- 229920002488 Hemicellulose Polymers 0.000 description 2
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- 235000013339 cereals Nutrition 0.000 description 2
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- 238000004090 dissolution Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 235000012054 meals Nutrition 0.000 description 2
- 239000001814 pectin Substances 0.000 description 2
- 229920001277 pectin Polymers 0.000 description 2
- 235000010987 pectin Nutrition 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 239000002904 solvent Substances 0.000 description 2
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- 241000196324 Embryophyta Species 0.000 description 1
- 229920001503 Glucan Polymers 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
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- 108010050181 aleurone Proteins 0.000 description 1
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- 230000015556 catabolic process Effects 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000012790 confirmation Methods 0.000 description 1
- 230000002354 daily effect Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000004134 energy conservation Methods 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 235000012631 food intake Nutrition 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 239000000446 fuel Substances 0.000 description 1
- 230000002496 gastric effect Effects 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- GPRLSGONYQIRFK-UHFFFAOYSA-N hydron Chemical compound [H+] GPRLSGONYQIRFK-UHFFFAOYSA-N 0.000 description 1
- 230000002218 hypoglycaemic effect Effects 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 210000002429 large intestine Anatomy 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 239000002932 luster Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000010358 mechanical oscillation Effects 0.000 description 1
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- 210000000813 small intestine Anatomy 0.000 description 1
- 239000002195 soluble material Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 239000003760 tallow Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/20—Reducing nutritive value; Dietetic products with reduced nutritive value
- A23L33/21—Addition of substantially indigestible substances, e.g. dietary fibres
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/30—Physical treatment, e.g. electrical or magnetic means, wave energy or irradiation
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/30—Physical treatment, e.g. electrical or magnetic means, wave energy or irradiation
- A23L5/32—Physical treatment, e.g. electrical or magnetic means, wave energy or irradiation using phonon wave energy, e.g. sound or ultrasonic waves
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/30—Physical treatment, e.g. electrical or magnetic means, wave energy or irradiation
- A23L5/34—Physical treatment, e.g. electrical or magnetic means, wave energy or irradiation using microwaves
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Life Sciences & Earth Sciences (AREA)
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- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
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- Mycology (AREA)
- Medicines Containing Plant Substances (AREA)
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Abstract
The present invention relates to a kind of method of modifying of little rice bran water-soluble dietary fiber, the method for modifying of this little rice bran water-soluble dietary fiber：First, little rice bran is subjected to gas explosion pretreatment；2nd, processing is modified to the pretreated little rice bran of gas explosion using ultrasonic-microwave collaboration method；3rd, water-soluble dietary fiber is made using enzymatic gravimetric method：The reaction solution that step 2 is obtained is transferred in GDE enzyme culture digesters and digested, pH value 4.0 6.0,60 95 DEG C of hydrolysis temperature, the min of reaction time 20 30；Reactant after enzymolysis is transferred in CSF6 filters, alcohol precipitation, centrifugation after concentrating filter liquor, is freeze-dried to obtain little rice bran water-soluble dietary fiber powder, water soluble dietary fiber content is up to 10.3% in little rice bran water-soluble dietary fiber powder.The present invention is substantially increased the content of little rice bran water-soluble dietary fiber, reaches the requirement of high-quality dietary fiber, efficiently solves the problem of little rice bran utilization rate is low.
Description
First, technical field
The present invention relates to foodstuff processing technology field, and in particular to a kind of modification side of little rice bran water-soluble dietary fiber
Method.
2nd, background technology
With the lifting of our people's standard of living, the high heat such as great variety, animal tallow also occur for everybody dietary structure
Food and protein intake are excessive, and coarse food grain intake is fewer and fewer, causes all kinds of patients with chronic diseases more and more.Dietary fiber
Some physiological actions obtained scientific experimentation confirmation, be such as beneficial to gastrointestinal health, pre- preventing obesity, reduce angiocardiopathy and
Sick hair rate of diabetes etc..In order to prevent the generation of chronic disease, it should suitably increase the intake of dietary fiber.U.S.'s food and medicine
Product management board（FDA）The reasonable intake recommended is the daily 20-35g of adult；Nutrition Society of China is according to China's national physique
The reasonable intake recommended is 30.2g for each person every day.
Dietary fiber is referred to as being beneficial to man the 7th nutrient of health.American Association of Cereal Chemists is defined as
" dietary fiber be it is a kind of in human small intestine's digestion resistant, absorb, in the plant edible part or similar that large intestine ferments completely or partially
Carbohydrate ".According to its dissolubility different demarcation into insoluble diedairy fiber and water-soluble dietary fiber.Aqueous soluble dietary is fine
Dimension includes the materials such as water-soluble hemicellulose, glucan, oligosaccharide and pectin, and water-soluble dietary fiber is adjusting blood glucose, blood fat,
Prevent angiocardiopathy etc. that there is good physiologically active.
Millet is the important rain fed crops in north of China region, and for cultivated area up to 1,400,000 mu, yield reaches ten thousand tons of 370-450.
Little rice bran is caused accessory substance in millet process, the mixture being made up of kind of a skin, aleurone and rice plumule, accounts for millet
The 6%-8% of quality.Little rice bran abundance and be renewable resource, in the past mainly as the feed of livestock, result in resource
Serious waste, and trigger environmental pollution.Containing abundant dietary fiber in little rice bran, but water soluble dietary fiber content is only
3% ~ 4%, it is impossible to reach the requirement of high quality meals fiber 10%, its function is subject to certain restrictions, and it is inclined to also result in production cost
It is high, it is difficult to realize large-scale industrial production, how to take effective means the large fragment DNA ligation key in dietary fiber is broken
Small molecule is cleaved into, so as to its loose close network structure, insoluble diedairy fiber is converted into water-soluble dietary fiber, is increased
The content of water-soluble dietary fiber, turn into the study hotspot in dietary fiber modification field.
3rd, the content of the invention
It is an object of the present invention to provide a kind of method of modifying of little rice bran water-soluble dietary fiber, this little rice bran are water-soluble
Property dietary fiber method of modifying be used for that to solve water soluble dietary fiber content in current little rice bran to be only 3% ~ 4%, it is impossible to reach
The problem of requirement of high quality meals fiber 10%.
To achieve these goals, the concrete technical scheme that the present invention uses is as follows：This little rice bran aqueous soluble dietary is fine
The method of modifying of dimension：
First, little rice bran is subjected to gas explosion pretreatment；
2nd, processing is modified to the pretreated little rice bran of gas explosion using ultrasound-microwave cooperating method：
Gas explosion pretreatment little rice bran is weighed, places in microwave and ultrasonic wave synergic extraction instrument, opens ultrasonic power, solid-liquid ratio 1:
20—1:60th, ultrasound-microwave cooperating time be the min of 20 min -60, microwave power be the W of 400 W -600, solid-liquid ratio unit
For g/mL；
3rd, water-soluble dietary fiber is made using enzyme-gravimetric method：
The reaction solution that step 2 is obtained is transferred in GDE enzyme culture digesters and digested, enzymatic hydrolysis condition：PH value 4.0-6.0,
Hydrolysis temperature is 60-95 DEG C, the reaction time is 20-30 min；Reactant after enzymolysis is transferred in CSF6 filters,
After concentrating filter liquor with 95% ethanol alcohol precipitation of 4 times of volumes, centrifugation, be freeze-dried to obtain little rice bran water-soluble dietary fiber powder, it is small
Water soluble dietary fiber content is up to 10.3% in rice bran water-soluble dietary fiber powder.
The method of little rice bran progress gas explosion pretreatment is in such scheme：
Little rice bran is added in gas explosion reactor and handled, it is respectively the MPa of 0.5MPa -1.5 to set gas explosion pressure, during gas explosion
Between be respectively 30s -90s, little rice bran through gas explosion pre-process after 40 DEG C drying, moisture control in the range of 6%~8%,
Gas explosion pretreatment little rice bran crushed 40 mesh sieves, and fat is removed through petroleum ether surname extraction.
The reaction solution that step 2 obtains in such scheme is transferred in GDE enzyme culture digesters, is then disappeared to GDE enzyme cultures
Change in device and add high temperature resistantα- amylase or neutral proteinase or amyloglucosidase are digested.
Such scheme is more preferably：
First, little rice bran is added in gas explosion reactor and handled.It is 1.0 MPa to set gas explosion pressure, and the gas explosion time is 90 s,
Little rice bran is pre-processed through gas explosion and controlled after 40 DEG C of drying, moisture in the range of 6%~8%；Gas explosion pre-processes millet chaff powder
The broken mesh sieve of mistake 40, fat is removed through petroleum ether surname extraction；
2nd, gas explosion pretreatment little rice bran is placed in microwave and ultrasonic wave synergic extraction instrument, opens ultrasonic power, solid-liquid ratio 1:50、
Ultrasound-microwave cooperating time is 57 min, microwave power is 535 W；
3rd, the reaction solution for obtaining step 2 is transferred in GDE enzyme culture digesters and digested, and regulation reacting liquid pH value is
4.5-7.0, temperature is 60 DEG C-95 DEG C, and the reaction time is the min of 20 min-30；Reactant after enzymolysis is transferred to CSF6
In filter, after concentrating filter liquor with 95% ethanol alcohol precipitation of 4 times of volumes, centrifugation, be freeze-dried to obtain little rice bran aqueous soluble dietary
Fiber dust, water soluble dietary fiber content reaches 10.841% in little rice bran water-soluble dietary fiber powder.
Beneficial effect：
1st, the present invention is substantially increased the content of little rice bran water-soluble dietary fiber, reaches wanting for high-quality dietary fiber
Ask, efficiently solve the problem of little rice bran utilization rate is low, improve little rice bran economic value added.
2nd, the present invention lays the foundation for the exploitation of water-soluble dietary fiber product, while improves millet accessory substance-little rice bran
Effective rate of utilization and economic value, there is important scientific guidance to be worth little rice bran comprehensive utilization and intensive processing.
3rd, the high-quality water-soluble dietary fiber powder color and luster that the present invention prepares is good, and water-soluble dietary fiber is in regulation blood
Sugared aspect has good physiologically active, is laid the foundation for exploitation hypoglycemic product.
4th, gas explosion pressure is 1.0 MPa in preferred embodiments of the present invention, because when gas explosion pressure is 1.0 MPa, little rice bran
Water soluble dietary fiber content dramatically increases.The little rice bran of gas explosion pretreatment is in spongy, and this is due to that gas explosion processing destruction is small
Some structure organizations such as fibre bundle of rice bran etc., the more conducively dissolution of purpose thing.And when pressure is higher than 1.0 MPa, little rice bran
Darken and result in the structure organization excessive degradation such as cellulose, hemicellulose, resolve into small-molecule substance, it is impossible to analyse with alcohol
Method alcohol precipitation, reduce the yield of little rice bran water-soluble dietary fiber.
5th, the present invention is combined the fuel factor of the mechanical oscillation effect of ultrasonic wave and microwave by ultrasound-microwave cooperating effect
Get up, be advantageous to water-soluble dietary fiber and raw material and be segregated into solvent, beneficial to the dissolution of water-soluble dietary fiber in material,
Have the advantages that energy-conservation, it is time saving, environmentally friendly.When implementing the method, pay attention to the single factor tests such as microwave power, solid-liquid ratio, time to water-soluble
The influence of property dietary fiber content.Microwave power is excessive, higher temperature damage water-soluble dietary fiber component, part aqueous
Property dietary fiber is decomposed into small-molecule substance, turns into alcohol-soluble material.Solvent load, which crosses conference, causes unit volume substrate content
It is relatively low, and the mass concentration of water-soluble dietary fiber in the solution diminishes, and is not easy alcohol precipitation, while cause ethanol during alcohol precipitation
Waste.Ultrasound-microwave cooperating action time is long, system temperature rise, and the part pectin in water-soluble dietary fiber component exists
Decomposed in the aqueous solution by hydrogen ion and reduce the yield of water-soluble dietary fiber.
4th, illustrate：
Fig. 1 is the process chart of the present invention.
5th, embodiment
The present invention is described further below in conjunction with the accompanying drawings：
Embodiment 1：
Refering to Fig. 1, the method for modifying of this little rice bran water-soluble dietary fiber is located in advance using red paddy little rice bran as raw material to it
Reason, water-soluble dietary fiber, tool finally then are made using enzyme-gravimetric method to its modification using ultrasound-microwave cooperating method
Body comprises the following steps：
（1）Weigh and handled in 500g little rice brans addition gas explosion reactor.It is 1.0 MPa to set gas explosion pressure, the gas explosion time
For 90s.Little rice bran is pre-processed through gas explosion and controlled after 40 DEG C of drying, moisture in the range of 6%~8%.Gas explosion pretreatment is small
Rice bran crushed 40 mesh sieves, and fat is removed through petroleum ether surname extraction.
（2）Gas explosion pretreatment little rice bran is weighed, places in microwave and ultrasonic wave synergic extraction instrument, opens ultrasonic power, solid-liquid ratio
For 1:60（g/mL）, ultrasound-microwave cooperating time be 50min, microwave power be 550 W.
（3）Reaction solution is transferred in GDE enzyme culture digesters, is separately added into appropriate high temperature resistantα- amylase, neutral egg
White enzyme, amyloglucosidase are digested, and regulation reacting liquid pH value is respectively 6.0,7.0,4.5, and sample temperature is respectively 95
DEG C, 60 DEG C, 60 DEG C, the reaction time is respectively 20min, 30min, 30min.Reactant after enzymolysis is transferred to CSF6 filters
In, after concentrating filter liquor with 95% ethanol alcohol precipitation of 4 times of volumes, centrifugation, be freeze-dried to obtain little rice bran water-soluble dietary fiber powder
End.Ultrasound-microwave cooperating is modified water soluble dietary fiber content in gas explosion pretreatment little rice bran and reaches 10.439%.
Embodiment 2：
（1）Weigh and handled in 500g little rice brans addition gas explosion reactor.It is 1.0 MPa to set gas explosion pressure, the gas explosion time
For 90s.Little rice bran is pre-processed through gas explosion and controlled after 40 DEG C of drying, moisture in the range of 6%~8%.Gas explosion pretreatment is small
Rice bran crushed 40 mesh sieves, and fat is removed through petroleum ether surname extraction.
（2）Gas explosion pretreatment little rice bran is weighed, places in microwave and ultrasonic wave synergic extraction instrument, opens ultrasonic power, solid-liquid ratio
For 1:50（g/mL）, ultrasound-microwave cooperating time be 50 min, microwave power be 500 W.
（3）Reaction solution is transferred in GDE enzyme culture digesters, is separately added into appropriate high temperature resistantα- amylase, neutral egg
White enzyme, amyloglucosidase are digested, and regulation reacting liquid pH value is respectively 6.0,7.0,4.5, and sample temperature is respectively 95
DEG C, 60 DEG C, 60 DEG C, the reaction time is respectively 20min, 30min, 30min.Reactant after enzymolysis is transferred to CSF6 filters
In, after concentrating filter liquor with 95% ethanol alcohol precipitation of 4 times of volumes, centrifugation, be freeze-dried to obtain little rice bran water-soluble dietary fiber powder
End.Ultrasound-microwave cooperating is modified water soluble dietary fiber content in gas explosion pretreatment little rice bran and reaches 10.332%.
Embodiment 3：
（1）Weigh and handled in 500g little rice brans addition gas explosion reactor.It is 1.0 MPa to set gas explosion pressure, the gas explosion time
For 90s.Little rice bran is pre-processed through gas explosion and controlled after 40 DEG C of drying, moisture in the range of 6%~8%.Gas explosion pretreatment is small
Rice bran crushed 40 mesh sieves, and fat is removed through petroleum ether surname extraction.
（2）Gas explosion pretreatment little rice bran is weighed, places in microwave and ultrasonic wave synergic extraction instrument, opens ultrasonic power, solid-liquid ratio
For 1:50（g/mL）, ultrasound-microwave cooperating time be 60 min, microwave power be 550 W.
（3）Reaction solution is transferred in GDE enzyme culture digesters, is separately added into appropriate high temperature resistantα- amylase, neutral egg
White enzyme, amyloglucosidase are digested, and regulation reacting liquid pH value is respectively 6.0,7.0,4.5, and sample temperature is respectively 95
DEG C, 60 DEG C, 60 DEG C, the reaction time is respectively 20min, 30min, 30min.Reactant after enzymolysis is transferred to CSF6 filters
In, after concentrating filter liquor with 95% ethanol alcohol precipitation of 4 times of volumes, centrifugation, be freeze-dried to obtain little rice bran water-soluble dietary fiber powder
End.Ultrasound-microwave cooperating is modified water soluble dietary fiber content in gas explosion pretreatment little rice bran and reaches 10.837%.
Embodiment 4：
（1）Weigh and handled in 500g little rice brans addition gas explosion reactor.It is 1.0 MPa to set gas explosion pressure, the gas explosion time
For 90s.Little rice bran is pre-processed through gas explosion and controlled after 40 DEG C of drying, moisture in the range of 6%~8%.Gas explosion pretreatment is small
Rice bran crushed 40 mesh sieves, and fat is removed through petroleum ether surname extraction.
（2）Gas explosion pretreatment little rice bran is weighed, places in microwave and ultrasonic wave synergic extraction instrument, opens ultrasonic power, solid-liquid ratio
For 1:50（g/mL）, ultrasound-microwave cooperating time be 57 min, microwave power be 535 W.
（3）Reaction solution is transferred in GDE enzyme culture digesters, is separately added into appropriate high temperature resistantα- amylase, neutral egg
White enzyme, amyloglucosidase are digested, and regulation reacting liquid pH value is respectively 6.0,7.0,4.5, and sample temperature is respectively 95
DEG C, 60 DEG C, 60 DEG C, the reaction time is respectively 20 min, 30 min, 30 min.Reactant after enzymolysis is transferred to CSF6 filterings
In device, after concentrating filter liquor with 95% ethanol alcohol precipitation of 4 times of volumes, centrifugation, be freeze-dried to obtain little rice bran water-soluble dietary fiber
Powder.Ultrasound-microwave cooperating is modified water soluble dietary fiber content in gas explosion pretreatment little rice bran and reaches 10.841%.The present invention
Method of modifying can be effectively increased the content of water-soluble dietary fiber.
Claims (4)
- A kind of 1. method of modifying of little rice bran water-soluble dietary fiber, it is characterised in that：This little rice bran water-soluble dietary fiber Method of modifying：First, little rice bran is subjected to gas explosion pretreatment；2nd, processing is modified to the pretreated little rice bran of gas explosion using ultrasound-microwave cooperating method：Gas explosion pretreatment little rice bran is weighed, places in microwave and ultrasonic wave synergic extraction instrument, opens ultrasonic power, solid-liquid ratio 1: 20—1:60th, ultrasound-microwave cooperating time be the min of 20 min -60, microwave power be the W of 400 W -600, solid-liquid ratio unit For g/mL；3rd, water-soluble dietary fiber is made using enzyme-gravimetric method：The reaction solution that step 2 is obtained is transferred in GDE enzyme culture digesters and digested, enzymatic hydrolysis condition：PH value 4.0-6.0, Hydrolysis temperature is 60-95 DEG C, the reaction time is 20-30 min；Reactant after enzymolysis is transferred in CSF6 filters, After concentrating filter liquor with 95% ethanol alcohol precipitation of 4 times of volumes, centrifugation, be freeze-dried to obtain little rice bran water-soluble dietary fiber powder, it is small Water soluble dietary fiber content is up to 10.3% in rice bran water-soluble dietary fiber powder.
- 2. the method for modifying of little rice bran water-soluble dietary fiber according to claim 1, it is characterised in that：Described millet Chaff carry out gas explosion pretreatment method be：Little rice bran is added in gas explosion reactor and handled, it is respectively the MPa of 0.5MPa -1.5 to set gas explosion pressure, during gas explosion Between be respectively 30s -90s, little rice bran through gas explosion pre-process after 40 DEG C drying, moisture control in the range of 6%~8%, Gas explosion pretreatment little rice bran crushed 40 mesh sieves, and fat is removed through petroleum ether surname extraction.
- 3. the method for modifying of little rice bran water-soluble dietary fiber according to claim 1 or 2, it is characterised in that：Described The reaction solution that step 2 obtains is transferred in GDE enzyme culture digesters, then adds high temperature resistant into GDE enzyme culture digestersα- Amylase or neutral proteinase or amyloglucosidase are digested.
- 4. the method for modifying of little rice bran water-soluble dietary fiber according to claim 3, it is characterised in that：First, little rice bran is added in gas explosion reactor and handled, it is 1.0 MPa to set gas explosion pressure, and the gas explosion time is 90 s, Little rice bran is pre-processed through gas explosion and controlled after 40 DEG C of drying, moisture in the range of 6%~8%；Gas explosion pre-processes millet chaff powder The broken mesh sieve of mistake 40, fat is removed through petroleum ether surname extraction；2nd, gas explosion pretreatment little rice bran is placed in microwave and ultrasonic wave synergic extraction instrument, opens ultrasonic power, solid-liquid ratio 1:50、 Ultrasound-microwave cooperating time is 57 min, microwave power is 535 W；3rd, the reaction solution for obtaining step 2 is transferred in GDE enzyme culture digesters and digested, and regulation reacting liquid pH value is 4.5-7.0, temperature is 60 DEG C-95 DEG C, and the reaction time is the min of 20 min-30；Reactant after enzymolysis is transferred to CSF6 In filter, after concentrating filter liquor with 95% ethanol alcohol precipitation of 4 times of volumes, centrifugation, be freeze-dried to obtain little rice bran aqueous soluble dietary Fiber dust, water soluble dietary fiber content reaches 10.841% in little rice bran water-soluble dietary fiber powder.
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| CN110301650A (en) * | 2019-08-05 | 2019-10-08 | 中南林业科技大学 | A kind of modified rice bran dietary fiber and its preparation method and application |
| CN111513257A (en) * | 2020-04-28 | 2020-08-11 | 南京素汇保健食品有限公司 | Preparation method of low-temperature coupling cooked oatmeal |
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