CN105029452A - Method used for extracting dietary fiber from wheat straw via biological method - Google Patents
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Abstract
The invention discloses a method used for extracting dietary fiber from wheat straw via a biological method. According to the method, an obtained dietary fiber product is creamy white; functional indexes are much higher than that required in dietary fiber standards of the western countries; product purity is 90% or higher; and the dietary fiber product can be taken as a high quality dietary fiber and ideal food additive. Advantages of the method are that: 1, dietary fiber in wheat straw is extracted via ultrasonic-assisted enzymolysis starch and protein removing, compound microbial fermentation, and ultrasonic-assisted enzymolysis modification, so that wheat straw dietary fiber yield is increased; 2, after ultrasonic-assisted enzymolysis and microbial fermentation, wheat straw cellulose and lignin degradation rate is higher than 70%, nutritional value of straw dietary fiber is increased obviously; and 3, production cost is relatively low, it is beneficial for industrialized production, a safe and feasible wheat straw dietary fiber development and utilization approach high in efficiency, and low in cost is established, and a novel approach is provided for further resource utilization of wheat straw.
Description
Technical field
The present invention relates to a kind of bioanalysis utilizes wheat stalk to extract the method for dietary fiber, belongs to food processing field.
Background technology
Dietary fiber is current a kind of functional food ingredient of generally acknowledging in the world, be called as " the 7th nutrient " except water, protein, carbohydrate, lipid, vitamin, the large nutrient of mineral matter 6, and be described as a kind of food additives of greatest concern in 21 century food industry, there is special health care and food modifying function.Dietary fiber can as the effective functional health-care food prevention of various diseases of one, and there is the special efficacies such as water-retaining property, Oil keeping, improvement food color and local flavor, human consumption can be improved absorb, strengthen body discharges function, promote the diet structure balance of human body, hinder human body to some eutrophic too much absorptions, keep function equilibrium, there is anticancer, step-down, reducing blood lipid, reducing slimming machine prevent the effects such as all kinds of calculus.
Wheat stalk is ripe wheat stalk, leaf, fringe part summation, is remainder after wheat maturation acquisition seed, accounts for crop quality 50%.China produces nearly 1.1 hundred million tons of wheat stalk every year, except minority is by as except animal feeding-stuff, farm manure and rural area fuel, major part is stacked or also field after directly burning, and effective industrial utilization proportion is extremely low, waste precious resources, and very big pollution is also caused to environment.Modern cereal science research finds, and wheat stalk contains and multiplely has antitumor, anti-oxidant important physiological activator, as dietary fiber, araboxylan, Dui – coumaric acid and forulic acid etc.; Wherein, in recent years general concern is caused to the Study and appliance of dietary fiber both at home and abroad, wheat stalk is made up of cellulose, hemicellulose, lignin, xylan and the material such as part glycoprotein and amino acid under native state, and the main component of these compositions dietary fiber just.Therefore, set up that a set of efficiency is higher, cost is lower, the wheat stalk dietary fiber development and utilization measure of safe and feasible, for the further recycling of wheat stalk provides new approach, to the economic value added increasing wheat planting industry, the industrial advantage giving full play to China's wheat has great importance.
Summary of the invention
The present invention is directed to the problems referred to above provides a kind of bioanalysis to utilize wheat stalk to extract the method for dietary fiber.
For achieving the above object, the technical solution used in the present invention is as follows:
The invention provides a kind of bioanalysis utilizes wheat stalk to extract the method for dietary fiber, it is characterized in that specifically comprising the following steps:
(1) wheat stalk pretreatment: pulverized by wheat stalk, crosses 100 mesh sieves, adds distilled water with the amount of feed liquid mass ratio 1:5 ~ 10, heats the enzyme that goes out, obtains wheat stalk suspension;
(2) phytic acid is decomposed: with citric acid adjustment pH of suspension to 5.0 ~ 6.0,45 ~ 60 DEG C are stirred 2 ~ 4 hours, decompose phytic acid, then wash with water to neutrality, and the weight ratio of adjustment wheat stalk and water is 1:8 ~ 1:10;
(3) ultrasound-assisted enzymolysis desizing: pH to 5.5 ~ 7.5 of adjustment wheat stalk suspension, α ~ amylase is added in the ratio of wheat stalk mass ratio 3 ~ 5%, enzymolysis under ultrasonic assistant effect, hydrolysis temperature 70 ~ 80 DEG C, ultrasonic power 300W, period adopts mixer at the uniform velocity to stir, and utilizes iodine fluid inspection to starch complete hydrolysis; Enzymolysis terminates rear filtration, and filtrate is starch hydrolyzate, adds the distilled water of 3 ~ 5 times of mass ratioes, obtain suspension in wheat stalk residue, for subsequent use;
(4) ultrasound-assisted enzymolysis deproteinized: pH to 8.0 ~ 9.0 of adjustment suspension, alkali protease is added in the ratio of wheat stalk mass ratio 2 ~ 3%, enzymolysis under ultrasonic assistant effect, hydrolysis temperature 50 ~ 60 DEG C, ultrasonic power 300W, period adopts mixer at the uniform velocity to stir, and enzymolysis time is 60 ~ 90min; Go out after enzymolysis terminates enzyme, and filter, filtrate is protein hydrolyzate, adds the distilled water flushing 2 ~ 3 times of 3 ~ 5 times of mass ratioes, filter to obtain wheat stalk residue in filter residue;
(5) bio-fermentation agent preparation: get 8 ~ 10 parts of adsorbents, 3 ~ 5 parts of calcium dihydrogen phosphates, 6 ~ 8 parts of glucose, 10 ~ 15 parts of rice brans, 10 ~ 15 portions of corn flour, 3 ~ 5 parts of rape cakes, 10 ~ 15 parts of wheat stalks, 2 ~ 3 parts of aspergillus nigers, 1 ~ 2 part of EM bacterium, 1 ~ 2 part of bacillus subtilis, mixing and stirring; Then moisture is adjusted to 35 ~ 40%, anaerobic fermentation 7 ~ 10d at 25 DEG C ~ 30 DEG C with the aqueous solution containing 2% molasses;
(6) batch mixing: wheat stalk residue 100 parts, bio-fermentation agent 3 ~ 5 parts, cellulase 0.5 ~ 1 part, zytase 0.5 ~ 1 part of ratio mix in mass ratio, adjustment moisture to 35 ~ 40%;
(7) anaerobic fermentation: by mixed mixture temperature control anaerobic fermentation 2 ~ 3d, temperature 28 ~ 32 DEG C, periodic agitation;
(8) dietary fiber modification: add 3 ~ 5 times of water after fermentation ends in fermentation residue, add the cellulase of fermentation residue mass fraction 3 ~ 5%, enzymolysis under ultrasonic assistant effect, hydrolysis temperature 50 ~ 60 DEG C, ultrasonic power 300W, period adopts mixer at the uniform velocity to stir, and enzymolysis time is 20 ~ 30min;
(9) alcohol is analysed: enzymolysis terminates the absolute ethyl alcohol precipitation 2 ~ 3h of rear use 5 ~ 8 times of volume ratios, collects filtration gained sediment and is modified wheat stalk dietary fiber;
(10) decolour: with the liquor natrii hypochloritis of concentration 0.8%, under 1:8 ~ 10 times volume ratio, pH6.0 condition, rinsing sediment 10 ~ 20min, is washed to neutrality;
(11) dry pulverizing: dry under 60 DEG C of conditions, pulverize, cross 100 mesh sieves, be i.e. obtained wheat stalk dietary fiber.
Adsorbent described in above-mentioned steps (5) is one or more in zeolite, active carbon, bentonite, vermiculite.
The invention provides a kind of bioanalysis utilizes wheat stalk to extract the method for dietary fiber, the diet fiber product color and luster extracted is rice white, product expansive force can reach 18.2mL/g, retention ability can reach 12.4%, far above its functional attributes of the standard diet fiber that western countries commonly use, product purity >=90%; Can be used as high-quality dietary fiber and desirable food additives.By to the toxic reaction after mouse gavaging dietary fiber, measure its LD
50to investigate the acute toxicity intensity of stalk dietary fiber in the short time, character and possible target organ.Test method adopts engler (Horn) method suddenly, and result shows, the median lethal dose LD of tested material
50be greater than 50g/kg.According to the acute toxicity dose grading standard of the chemical substance that GB15193.3-94 " acute toxicity test " promulgates, it is nontoxic that LD is greater than 15g/kg.
The invention provides a kind of bioanalysis utilizes wheat stalk to extract the method for dietary fiber, and dietary fiber productive rate more than 95%, is significantly higher than and reports technical merit at present; Carried out biologically active that is anti-oxidant and cholate vitro Adsorption to produced dietary fiber to study, result shows that the stalk dietary fiber extracted is to OH free radical and O
2 ~the IC of free radical scavenging activity
50value is respectively 1.75mg/mL and 2.35mg/mL, is 24.58mg/g to the vitro Adsorption ability of cholate, and functional result is higher than the stalk dietary fiber reported at present.In addition, result of the test shows that change processing step and process conditions have significant impact (p<0.01) to the extraction yield of dietary fiber and product functionality.
The invention provides the dietary fiber that a kind of bioanalysis utilizes wheat stalk to extract, it is advantageous that: one is adopt the fine dietary fiber in the technique extraction wheat stalk of ultrasound-assisted enzymolysis deproteinized starch+compound microorganism ferments+ultrasound-assisted enzymolysis modification, is extracted wheat stalk dietary fiber yield; Two be by ultrasound-assisted enzymolysis and fermentable after wheat stalk cellulose, Lignin degradation rate reach more than 70%, significantly improve the nutritive value of stalk dietary fiber; Three is that explained hereafter cost is lower, is beneficial to suitability for industrialized production, sets up that a set of efficiency is higher, cost is lower, the wheat stalk dietary fiber development and utilization measure of safe and feasible, for the further recycling of wheat stalk provides new approach.
Detailed description of the invention
embodiment 1:
Bioanalysis utilizes wheat stalk to extract a method for dietary fiber, it is characterized in that specifically comprising the following steps:
(1) wheat stalk pretreatment: pulverized by wheat stalk, crosses 100 mesh sieves, adds distilled water with the amount of feed liquid mass ratio 1:8, heats the enzyme that goes out, obtains wheat stalk suspension;
(2) phytic acid is decomposed: with citric acid adjustment pH of suspension to 5.0 ~ 6.0,45 ~ 60 DEG C are stirred 2 ~ 4 hours, decompose phytic acid, then wash with water to neutrality, and the weight ratio of adjustment wheat stalk and water is 1:9;
(3) ultrasound-assisted enzymolysis desizing: pH to 5.5 ~ 7.5 of adjustment wheat stalk suspension, α ~ amylase is added in the ratio of wheat stalk mass ratio 4%, enzymolysis under ultrasonic assistant effect, hydrolysis temperature 70 ~ 80 DEG C, ultrasonic power 300W, period adopts mixer at the uniform velocity to stir, and utilizes iodine fluid inspection to starch complete hydrolysis; Enzymolysis terminates rear filtration, and filtrate is starch hydrolyzate, adds the distilled water of 4 times of mass ratioes, obtain suspension in wheat stalk residue, for subsequent use;
(4) ultrasound-assisted enzymolysis deproteinized: pH to 8.0 ~ 9.0 of adjustment suspension, alkali protease is added in the ratio of wheat stalk mass ratio 2.5%, enzymolysis under ultrasonic assistant effect, hydrolysis temperature 50 ~ 60 DEG C, ultrasonic power 300W, period adopts mixer at the uniform velocity to stir, and enzymolysis time is 70min; Go out after enzymolysis terminates enzyme, and filter, filtrate is protein hydrolyzate, adds the distilled water flushing 2 times of 4 times of mass ratioes, filter to obtain wheat stalk residue in filter residue;
(5) bio-fermentation agent preparation: get 9 parts of zeolites, 4 parts of calcium dihydrogen phosphates, 7 parts of glucose, 13 parts of rice brans, 12 portions of corn flour, 4 parts of rape cakes, 12 parts of wheat stalks, 2.5 parts of aspergillus nigers, 1.5 parts of EM bacterium, 1.5 parts of bacillus subtilises, mixing and stirring; Then moisture is adjusted to 35 ~ 40%, anaerobic fermentation 8d at 25 DEG C ~ 30 DEG C with the aqueous solution containing 2% molasses;
(6) batch mixing: wheat stalk residue 100 parts, bio-fermentation agent 4 parts, cellulase 0.8 part, zytase 0.7 part of ratio mix in mass ratio, adjustment moisture to 35 ~ 40%;
(7) anaerobic fermentation: by mixed mixture temperature control anaerobic fermentation 2.5d, temperature 28 ~ 32 DEG C, periodic agitation;
(8) dietary fiber modification: add 4 times of water after fermentation ends in fermentation residue, add the cellulase of fermentation residue mass fraction 4%, enzymolysis under ultrasonic assistant effect, hydrolysis temperature 50 ~ 60 DEG C, ultrasonic power 300W, period adopts mixer at the uniform velocity to stir, and enzymolysis time is 25min;
(9) alcohol is analysed: enzymolysis terminates the absolute ethyl alcohol precipitation 2.5h of rear use 6 times of volume ratios, collects filtration gained sediment and is modified wheat stalk dietary fiber;
(10) decolour: with the liquor natrii hypochloritis of concentration 0.8%, under 1:9 times of volume ratio, pH6.0 condition, rinsing sediment 15min, is washed to neutrality;
(11) dry pulverizing: dry under 60 DEG C of conditions, pulverize, cross 100 mesh sieves, be i.e. obtained wheat stalk dietary fiber.
embodiment 2:
Bioanalysis utilizes wheat stalk to extract a method for dietary fiber, specifically comprises the following steps:
(1) wheat stalk pretreatment: pulverized by wheat stalk, crosses 100 mesh sieves, adds distilled water with the amount of feed liquid mass ratio 1:10, heats the enzyme that goes out, obtains wheat stalk suspension;
(2) phytic acid is decomposed: with citric acid adjustment pH of suspension to 5.0 ~ 6.0,45 ~ 60 DEG C are stirred 2 hours, decompose phytic acid, then wash with water to neutrality, and the weight ratio of adjustment wheat stalk and water is 1:10;
(3) ultrasound-assisted enzymolysis desizing: pH to 5.5 ~ 7.5 of adjustment wheat stalk suspension, α ~ amylase is added in the ratio of wheat stalk mass ratio 5%, enzymolysis under ultrasonic assistant effect, hydrolysis temperature 70 ~ 80 DEG C, ultrasonic power 300W, period adopts mixer at the uniform velocity to stir, and utilizes iodine fluid inspection to starch complete hydrolysis; Enzymolysis terminates rear filtration, and filtrate is starch hydrolyzate, adds the distilled water of 5 times of mass ratioes, obtain suspension in wheat stalk residue, for subsequent use;
(4) ultrasound-assisted enzymolysis deproteinized: pH to 8.0 ~ 9.0 of adjustment suspension, alkali protease is added in the ratio of wheat stalk mass ratio 3%, enzymolysis under ultrasonic assistant effect, hydrolysis temperature 50 ~ 60 DEG C, ultrasonic power 300W, period adopts mixer at the uniform velocity to stir, and enzymolysis time is 60min; Go out after enzymolysis terminates enzyme, and filter, filtrate is protein hydrolyzate, adds the distilled water flushing 2 times of 5 times of mass ratioes, filter to obtain wheat stalk residue in filter residue;
(5) bio-fermentation agent preparation: get 10 parts of active carbons, 3 parts of calcium dihydrogen phosphates, 8 parts of glucose, 10 parts of rice brans, 15 portions of corn flour, 3 parts of rape cakes, 15 parts of wheat stalks, 2 parts of aspergillus nigers, 2 parts of EM bacterium, 1 part of bacillus subtilis, mixing and stirring; Then moisture is adjusted to 35 ~ 40%, anaerobic fermentation 10d at 25 DEG C ~ 30 DEG C with the aqueous solution containing 2% molasses;
(6) batch mixing: wheat stalk residue 100 parts, bio-fermentation agent 5 parts, cellulase 1 part, zytase 1 part of ratio mix in mass ratio, adjustment moisture to 35 ~ 40%;
(7) anaerobic fermentation: by mixed mixture temperature control anaerobic fermentation 2d, temperature 28 ~ 32 DEG C, periodic agitation;
(8) dietary fiber modification: add 5 times of water after fermentation ends in fermentation residue, add the cellulase of fermentation residue mass fraction 5%, enzymolysis under ultrasonic assistant effect, hydrolysis temperature 50 ~ 60 DEG C, ultrasonic power 300W, period adopts mixer at the uniform velocity to stir, and enzymolysis time is 20min;
(9) alcohol is analysed: enzymolysis terminates the absolute ethyl alcohol precipitation 2h of rear use 8 times of volume ratios, collects filtration gained sediment and is modified wheat stalk dietary fiber;
(10) decolour: with the liquor natrii hypochloritis of concentration 0.8%, under 1:10 times of volume ratio, pH6.0 condition, rinsing sediment 10min, is washed to neutrality;
(11) dry pulverizing: dry under 60 DEG C of conditions, pulverize, cross 100 mesh sieves, be i.e. obtained wheat stalk dietary fiber.
embodiment 3:
Bioanalysis utilizes wheat stalk to extract a method for dietary fiber, it is characterized in that specifically comprising the following steps:
(1) wheat stalk pretreatment: pulverized by wheat stalk, crosses 100 mesh sieves, adds distilled water with the amount of feed liquid mass ratio 1:5, heats the enzyme that goes out, obtains wheat stalk suspension;
(2) phytic acid is decomposed: with citric acid adjustment pH of suspension to 5.0 ~ 6.0,45 ~ 60 DEG C are stirred 4 hours, decompose phytic acid, then wash with water to neutrality, and the weight ratio of adjustment wheat stalk and water is 1:8;
(3) ultrasound-assisted enzymolysis desizing: pH to 5.5 ~ 7.5 of adjustment wheat stalk suspension, α ~ amylase is added in the ratio of wheat stalk mass ratio 3%, enzymolysis under ultrasonic assistant effect, hydrolysis temperature 70 ~ 80 DEG C, ultrasonic power 300W, period adopts mixer at the uniform velocity to stir, and utilizes iodine fluid inspection to starch complete hydrolysis; Enzymolysis terminates rear filtration, and filtrate is starch hydrolyzate, adds the distilled water of 3 times of mass ratioes, obtain suspension in wheat stalk residue, for subsequent use;
(4) ultrasound-assisted enzymolysis deproteinized: pH to 8.0 ~ 9.0 of adjustment suspension, alkali protease is added in the ratio of wheat stalk mass ratio 2%, enzymolysis under ultrasonic assistant effect, hydrolysis temperature 50 ~ 60 DEG C, ultrasonic power 300W, period adopts mixer at the uniform velocity to stir, and enzymolysis time is 90min; Go out after enzymolysis terminates enzyme, and filter, filtrate is protein hydrolyzate, adds the distilled water flushing 3 times of 3 times of mass ratioes, filter to obtain wheat stalk residue in filter residue;
(5) bio-fermentation agent preparation: get 8 parts of bentonites, 5 parts of calcium dihydrogen phosphates, 6 parts of glucose, 15 parts of rice brans, 10 portions of corn flour, 5 parts of rape cakes, 10 parts of wheat stalks, 3 parts of aspergillus nigers, 1 part of EM bacterium, 2 parts of bacillus subtilises, mixing and stirring; Then moisture is adjusted to 35 ~ 40%, anaerobic fermentation 10d at 25 DEG C ~ 30 DEG C with the aqueous solution containing 2% molasses;
(6) batch mixing: wheat stalk residue 100 parts, bio-fermentation agent 3 parts, cellulase 0.5 part, zytase 0.5 part of ratio mix in mass ratio, adjustment moisture to 35 ~ 40%;
(7) anaerobic fermentation: by mixed mixture temperature control anaerobic fermentation 3d, temperature 28 ~ 32 DEG C, periodic agitation;
(8) dietary fiber modification: add 3 times of water after fermentation ends in fermentation residue, add the cellulase of fermentation residue mass fraction 3%, enzymolysis under ultrasonic assistant effect, hydrolysis temperature 50 ~ 60 DEG C, ultrasonic power 300W, period adopts mixer at the uniform velocity to stir, and enzymolysis time is 30min;
(9) alcohol is analysed: enzymolysis terminates the absolute ethyl alcohol precipitation 3h of rear use 5 times of volume ratios, collects filtration gained sediment and is modified wheat stalk dietary fiber;
(10) decolour: with the liquor natrii hypochloritis of concentration 0.8%, under 1:8 times of volume ratio, pH6.0 condition, rinsing sediment 20min, is washed to neutrality;
(11) dry pulverizing: dry under 60 DEG C of conditions, pulverize, cross 100 mesh sieves, be i.e. obtained wheat stalk dietary fiber.
Above embodiment only for illustration of technical scheme of the present invention, but not is limited; Although be described in detail by invention with reference to previous embodiment, for the person of ordinary skill of the art, still the technical scheme described in previous embodiment can be modified, or equivalent replacement has been carried out to wherein portion of techniques feature; And to these amendments or replacement, do not make the essence of appropriate technical solution depart from the spirit and scope of the present invention's technical scheme required for protection.
Claims (2)
1. bioanalysis utilizes wheat stalk to extract a method for dietary fiber, it is characterized in that specifically comprising the following steps:
(1) wheat stalk pretreatment: pulverized by wheat stalk, crosses 100 mesh sieves, adds distilled water with the amount of feed liquid mass ratio 1:5 ~ 10, heats the enzyme that goes out, obtains wheat stalk suspension;
(2) phytic acid is decomposed: with citric acid adjustment pH of suspension to 5.0 ~ 6.0,45 ~ 60 DEG C are stirred 2 ~ 4 hours, decompose phytic acid, then wash with water to neutrality, and the weight ratio of adjustment wheat stalk and water is 1:8 ~ 1:10;
(3) ultrasound-assisted enzymolysis desizing: pH to 5.5 ~ 7.5 of adjustment wheat stalk suspension, α ~ amylase is added in the ratio of wheat stalk mass ratio 3 ~ 5%, enzymolysis under ultrasonic assistant effect, hydrolysis temperature 70 ~ 80 DEG C, ultrasonic power 300W, period adopts mixer at the uniform velocity to stir, and utilizes iodine fluid inspection to starch complete hydrolysis; Enzymolysis terminates rear filtration, and filtrate is starch hydrolyzate, adds the distilled water of 3 ~ 5 times of mass ratioes, obtain suspension in wheat stalk residue, for subsequent use;
(4) ultrasound-assisted enzymolysis deproteinized: pH to 8.0 ~ 9.0 of adjustment suspension, alkali protease is added in the ratio of wheat stalk mass ratio 2 ~ 3%, enzymolysis under ultrasonic assistant effect, hydrolysis temperature 50 ~ 60 DEG C, ultrasonic power 300W, period adopts mixer at the uniform velocity to stir, and enzymolysis time is 60 ~ 90min; Go out after enzymolysis terminates enzyme, and filter, filtrate is protein hydrolyzate, adds the distilled water flushing 2 ~ 3 times of 3 ~ 5 times of mass ratioes, filter to obtain wheat stalk residue in filter residue;
(5) bio-fermentation agent preparation: get 8 ~ 10 parts of adsorbents, 3 ~ 5 parts of calcium dihydrogen phosphates, 6 ~ 8 parts of glucose, 10 ~ 15 parts of rice brans, 10 ~ 15 portions of corn flour, 3 ~ 5 parts of rape cakes, 10 ~ 15 parts of wheat stalks, 2 ~ 3 parts of aspergillus nigers, 1 ~ 2 part of EM bacterium, 1 ~ 2 part of bacillus subtilis, mixing and stirring; Then moisture is adjusted to 35 ~ 40%, anaerobic fermentation 7 ~ 10d at 25 DEG C ~ 30 DEG C with the aqueous solution containing 2% molasses;
(6) batch mixing: wheat stalk residue 100 parts, bio-fermentation agent 3 ~ 5 parts, cellulase 0.5 ~ 1 part, zytase 0.5 ~ 1 part of ratio mix in mass ratio, adjustment moisture to 35 ~ 40%;
(7) anaerobic fermentation: by mixed mixture temperature control anaerobic fermentation 2 ~ 3d, temperature 28 ~ 32 DEG C, periodic agitation;
(8) dietary fiber modification: add 3 ~ 5 times of water after fermentation ends in fermentation residue, add the cellulase of fermentation residue mass fraction 3 ~ 5%, enzymolysis under ultrasonic assistant effect, hydrolysis temperature 50 ~ 60 DEG C, ultrasonic power 300W, period adopts mixer at the uniform velocity to stir, and enzymolysis time is 20 ~ 30min;
(9) alcohol is analysed: enzymolysis terminates the absolute ethyl alcohol precipitation 2 ~ 3h of rear use 5 ~ 8 times of volume ratios, collects filtration gained sediment and is modified wheat stalk dietary fiber;
(10) decolour: with the liquor natrii hypochloritis of concentration 0.8%, under 1:8 ~ 10 times volume ratio, pH6.0 condition, rinsing sediment 10 ~ 20min, is washed to neutrality;
(11) dry pulverizing: dry under 60 DEG C of conditions, pulverize, cross 100 mesh sieves, be i.e. obtained wheat stalk dietary fiber.
2. a kind of bioanalysis according to claim 1 utilizes wheat stalk to extract the method for dietary fiber, it is characterized in that described adsorbent is one or more in zeolite, active carbon, bentonite, vermiculite.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106173877A (en) * | 2016-07-25 | 2016-12-07 | 滨州中裕食品有限公司 | A kind of high-valued comprehensive utilization Testa Tritici prepares the new technology of dietary fiber |
| CN106262952A (en) * | 2016-07-27 | 2017-01-04 | 南京正宽医药科技有限公司 | A kind of wheat edible fiber extracting method and grain dietary fiber Antilipemic slimming tea |
| CN106262955A (en) * | 2016-09-24 | 2017-01-04 | 青岛嘉瑞生物技术有限公司 | A kind of method preparing Herba suadeae glaucae dietary fiber |
| CN106387925A (en) * | 2016-09-16 | 2017-02-15 | 青岛嘉瑞生物技术有限公司 | Method for preparing dietary fibers from salicornia stalks by biological method |
| CN107788243A (en) * | 2017-10-31 | 2018-03-13 | 北京小小牧人生物科技有限公司 | A kind of high microsteping sow feed and preparation method and application |
| CN107927321A (en) * | 2017-10-31 | 2018-04-20 | 湖南农业大学 | A kind of natural concentration dietary fiber and preparation method thereof |
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| CN102450648A (en) * | 2010-10-18 | 2012-05-16 | 华中农业大学 | Extraction method for dietary fiber from citrus peel residues |
| CN104247733A (en) * | 2014-10-10 | 2014-12-31 | 青岛嘉瑞生物技术有限公司 | Nutritional health bread rich in wheat bran dietary fiber and preparation method of nutritional health bread |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102450648A (en) * | 2010-10-18 | 2012-05-16 | 华中农业大学 | Extraction method for dietary fiber from citrus peel residues |
| CN104247733A (en) * | 2014-10-10 | 2014-12-31 | 青岛嘉瑞生物技术有限公司 | Nutritional health bread rich in wheat bran dietary fiber and preparation method of nutritional health bread |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106173877A (en) * | 2016-07-25 | 2016-12-07 | 滨州中裕食品有限公司 | A kind of high-valued comprehensive utilization Testa Tritici prepares the new technology of dietary fiber |
| CN106262952A (en) * | 2016-07-27 | 2017-01-04 | 南京正宽医药科技有限公司 | A kind of wheat edible fiber extracting method and grain dietary fiber Antilipemic slimming tea |
| CN106387925A (en) * | 2016-09-16 | 2017-02-15 | 青岛嘉瑞生物技术有限公司 | Method for preparing dietary fibers from salicornia stalks by biological method |
| CN106262955A (en) * | 2016-09-24 | 2017-01-04 | 青岛嘉瑞生物技术有限公司 | A kind of method preparing Herba suadeae glaucae dietary fiber |
| CN106262955B (en) * | 2016-09-24 | 2020-06-19 | 深圳市益百分实业有限公司 | Method for preparing common seepweed herb dietary fiber |
| CN107788243A (en) * | 2017-10-31 | 2018-03-13 | 北京小小牧人生物科技有限公司 | A kind of high microsteping sow feed and preparation method and application |
| CN107927321A (en) * | 2017-10-31 | 2018-04-20 | 湖南农业大学 | A kind of natural concentration dietary fiber and preparation method thereof |
| CN107927321B (en) * | 2017-10-31 | 2021-05-18 | 湖南农业大学 | Natural concentrated dietary fiber and preparation method thereof |
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