CN107927321A - A kind of natural concentration dietary fiber and preparation method thereof - Google Patents
A kind of natural concentration dietary fiber and preparation method thereof Download PDFInfo
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- CN107927321A CN107927321A CN201711043437.3A CN201711043437A CN107927321A CN 107927321 A CN107927321 A CN 107927321A CN 201711043437 A CN201711043437 A CN 201711043437A CN 107927321 A CN107927321 A CN 107927321A
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- dietary fiber
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- 235000013325 dietary fiber Nutrition 0.000 title claims abstract description 56
- 238000002360 preparation method Methods 0.000 title claims abstract description 22
- 235000010204 pine bark Nutrition 0.000 claims abstract description 41
- 239000000463 material Substances 0.000 claims abstract description 37
- 240000006024 Lactobacillus plantarum Species 0.000 claims abstract description 32
- 235000013965 Lactobacillus plantarum Nutrition 0.000 claims abstract description 32
- 229940072205 lactobacillus plantarum Drugs 0.000 claims abstract description 32
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 32
- 239000002994 raw material Substances 0.000 claims abstract description 22
- 238000000855 fermentation Methods 0.000 claims abstract description 21
- 230000004151 fermentation Effects 0.000 claims abstract description 21
- 235000005911 diet Nutrition 0.000 claims abstract description 20
- 238000002604 ultrasonography Methods 0.000 claims abstract description 14
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- 239000002351 wastewater Substances 0.000 claims abstract description 8
- 238000001035 drying Methods 0.000 claims abstract description 6
- 238000001816 cooling Methods 0.000 claims abstract description 5
- 238000007873 sieving Methods 0.000 claims abstract description 5
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- JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 1
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- IAJILQKETJEXLJ-UHFFFAOYSA-N Galacturonsaeure Natural products O=CC(O)C(O)C(O)C(O)C(O)=O IAJILQKETJEXLJ-UHFFFAOYSA-N 0.000 description 1
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- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
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- AEMOLEFTQBMNLQ-BKBMJHBISA-N alpha-D-galacturonic acid Chemical compound O[C@H]1O[C@H](C(O)=O)[C@H](O)[C@H](O)[C@H]1O AEMOLEFTQBMNLQ-BKBMJHBISA-N 0.000 description 1
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- BVTBRVFYZUCAKH-UHFFFAOYSA-L disodium selenite Chemical compound [Na+].[Na+].[O-][Se]([O-])=O BVTBRVFYZUCAKH-UHFFFAOYSA-L 0.000 description 1
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- 230000008855 peristalsis Effects 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 239000010318 polygalacturonic acid Substances 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- -1 polysaccharide carbohydrate Chemical class 0.000 description 1
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- 238000000926 separation method Methods 0.000 description 1
- 235000021391 short chain fatty acids Nutrition 0.000 description 1
- 150000004666 short chain fatty acids Chemical class 0.000 description 1
- 210000000813 small intestine Anatomy 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 229960001471 sodium selenite Drugs 0.000 description 1
- 239000011781 sodium selenite Substances 0.000 description 1
- 235000015921 sodium selenite Nutrition 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
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- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/14—Pretreatment of feeding-stuffs with enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/111—Aromatic compounds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/169—Plantarum
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Food Science & Technology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Physiology (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Botany (AREA)
- Mycology (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
A kind of natural concentration dietary fiber and preparation method thereof, the natural concentration dietary fiber is the pine bark grinding and sieving that will be enriched in dietary cellulosic, soak, waste water is exhausted after having soaked, then the pine bark after immersion is transferred in reactor, add water, obtain pine bark mixed liquor, carry out ultrasound-assisted enzymolysis processing, draining liq after enzymolysis, the material access lactobacillus plantarum HKB 38 after enzymolysis is then fermented, the material after fermentation is carried out high temperature and pressure heat treatment, cooling, drying, up to natural concentration dietary fiber.Present invention additionally comprises the preparation method of natural concentration dietary fiber.The present invention selects cheap and resourceful pine bark while substantially reducing production cost, to make full use of resource as raw materials for production, create high added value.Invention significantly improves the purity of dietary fiber and the swellability of fiber product and retention ability.It is demonstrated experimentally that crude fiber content >=60% in present invention concentration dietary fiber, neutral detergent fiber (NDF) content >=86%, acid detergent fiber (ADF) content >=70%, product swellability >=5.0mL/g, retention ability >=5.0g/g.
Description
Technical field
The present invention relates to a kind of dietary fiber and preparation method thereof, and in particular to a kind of natural concentration dietary fiber and its system
Preparation Method.
Background technology
Dietary fiber is the general designation for the polysaccharide carbohydrate and lignin do not digested and assimilated by human body, its physiology
Function is mainly following several respects：1) promote gastrointestinal peristalsis, reduce constipation；2) street cleaner act on, reduce pathogen from hindgut to
Anterior intestine shifts, and some constituent structure of dietary fiber also have the function that absorbing toxin and pathogen；3) dietary fiber can be steady
Determine blood sugar concentration, reduce blood cholesterol levels；4) solubility/dietary fiber easy to ferment is fermented in colon, and is knot
Microorganism provides nutrition in intestines, is conducive to stablize intestinal flora balance；5) short chain fatty acids (acetic acid, third that fiber fermentation produces
Acid and butyric acid) suppress the growth of pathogen in turn, in addition butyric acid can provide energy for colonic mucosal cells, and promotion epithelium is thin
The propagation of born of the same parents, maintains the integrality of intestinal mucosa, so as to be beneficial to the absorption of nutrient and pre- anti-diarrhea；6) dietary fiber has water holding
The characteristic of expansion, can strengthen satiety, reduce the generation of abnormal behaviour caused by animal starvation.
Between past 20 years, the use of dietary fiber is received more and more attention in animals and humans field of nutrition.Meals
Although fiber cannot directly provide nutrition, there is sanatory physiological property, dietary fiber is by domestic and international medicine at present
It is considered as " the 7th nutrient " with nutrition expert.Fermented, dilatancy, retentiveness, absorption especially possessed by dietary fiber
The positive effect that the physiological property such as property and ion exchange is it in animal provides physiological foundation.More and more researchs
It was found that suitable dietary fiber is non-but not damages animal health, its growth situation can also be improved and improve production performance, be
While animal energy is provided, moreover it is possible to form rational daily ration structure, maintain the normal stool function of animal body.Dietary fiber
Also there is certain preventive and therapeutic action to the common intestines problem such as irritable bowel syndrome, inflammatory bowel disease and intestinal cancer, favorably
In keeping enteron aisle normal physiological function, repair and improve damaged intestine environment, for safeguarding that intestinal health is significant.
At present, common fiber source has the conventional feeds such as wheat bran, soybean skin, megasse, corn bran original in feed
Expect, not only fiber content is relatively low (crude fiber content is usually no more than 10% in such as wheat bran) in these crude fibre raw materials, additive amount
It is poor that feed palatability is easily led to when high；And insoluble fibre content is higher in these raw materials, it is difficult to be fermented, cause utilization rate
It is low.The dietary fiber that content of cellulose is high, physicochemical property is good is prepared to get growing concern for, such as CN105124134A
A kind of method for preparing dietary fiber is disclosed, this method comprises the following steps：1) raw material containing dietary cellulosic is crushed
After sieve, soak, obtain substrate solution；2) substrate solution is emulsified, the substrate solution after being emulsified；3) to after emulsification
Add enzyme in substrate solution to be digested, after gained enzymolysis liquid enzyme deactivation, centrifugation, collects precipitation, obtain filtering medium；4) connect into filtering medium
After entering probiotics fermention, sterilize, it is dry, obtain the dietary fiber.
Pine bark as a kind of resource for creating high added value ignored for a long time by people, not only rich in procyanidine etc. into
Point, while also can be as the quality raw materials of extraction/preparation concentration dietary fiber, but spent relative to disclosed in CN105124134A
For the raw materials such as raw shell, rice husk, corncob, pine bark is more hard, degree of lignification higher.Therefore, if wanting to utilize pine bark
Dietary fiber is prepared as raw material, it is necessary to is improved, can be played for dietary fiber preparation method and technological parameter
The effect of pine bark.
The content of the invention
The purpose of the present invention is what is be achieved through the following technical solutions, a lactobacillus plantarum (Lactobacillus
Plantarum) HKB-38, China typical culture collection center (abbreviation CCTCC), preservation are preserved on December 8th, 2016
Numbering is CCTCC NO:M2016727, depositary institution address：Chinese Wuhan Wuhan Universitys, postcode 430072.
Further, the lactobacillus plantarum HKB-38, its 16S rRNA gene order such as SEQ ID NO:Shown in 1.
Further aim of the present invention is achieved through the following technical solutions, a kind of natural concentration dietary fiber, by richness
Pine bark (preferably Pissodss sp. on Yunnan Pine, bark of dahurian larch or Masson Pine Bark, more preferably Pissodss sp. on Yunnan Pine) containing dietary cellulosic
Grinding and sieving, adds water (preferably room temperature clear water) to soak, waste water is exhausted after having soaked, is then transferred to the pine bark after immersion
In reactor, water (preferably room temperature clear water) is added again, obtains pine bark mixed liquor, carries out ultrasound-assisted enzymolysis processing, enzyme
Draining liq after solution, then ferments the material access lactobacillus plantarum HKB-38 after enzymolysis, finally by the thing after fermentation
Material carries out high temperature and pressure heat treatment, cooling, drying, up to natural concentration dietary fiber.
Further aim of the present invention is achieved through the following technical solutions, a kind of side of natural concentration dietary fiber
Method, comprises the following steps：
1) by after the pine bark raw material cleaning removal of impurities containing dietary cellulosic, 20~60 mesh sieves are crushed, according to sieving pine
The ratio of bark and water is 1g：5~20mL, adds water and soaks 2~10h, waste water is exhausted after having soaked；The step is soaked can be with
Some water-solubility impurities, such as soil, dust, water soluble protein and carbohydrate etc. are removed, is conducive to subsequently digest, carries
The purity of high dietary-fiber product；
2) pine bark after immersion obtained by step 1) is transferred in reactor, it is clear according to the pine bark after immersion and room temperature
The ratio of water is 1g：10~30mL, adds water again, obtains pine bark mixed liquor, carries out ultrasound-assisted enzymolysis processing, after enzymolysis
Draining liq, the material after being digested；
3) into the material after enzymolysis obtained by step 2), access lactobacillus plantarum HKB-38 ferments, after being fermented
Material；
4) it is 350~400 DEG C, under conditions of pressure is 5~30MPa the material after fermentation obtained by step 3) to be placed in temperature
100~60min is heat-treated, cools down, is dry,.
Further, in step 2), the ultrasound-assisted enzymolysis processing is 2~10min of first ultrasound, then adds enzyme preparation
Digested, hydrolysis temperature is 35~55 DEG C, and enzymolysis pH value is 6.0~8.0, and enzymolysis time is 20~60min.
Further, the enzyme preparation is pectase or/and papain；The enzyme activity of the pectase is 0.5~5.0U/
The ratio of mg (preferably 1.0U/mg), pectase and pine bark mixed liquor is 1~2g：1L；The enzyme activity of the papain for 5~
The ratio of 50U/mg (preferably 12U/mg), papain and pine bark mixed liquor is 5~10g：1L.Enzyme activity on pectase
It is defined as follows：Under the conditions of 50 DEG C and pH value are 4.0,1min decomposes polygalacturonic acid and produces needed for 1 μm of ol galacturonic acid
Enzyme amount, be 1 enzyme activity unit；The enzyme activity of papain is defined as follows：In 40 DEG C and the condition of pH value 7.2
Under, 1min caseinhydrolysates generate the amount of the enzyme needed for 1 μ g tyrosine, are 1 enzyme activity unit.
Further, in step 3), the mass ratio of the lactobacillus plantarum HKB-38 and the material after enzymolysis are 1~3g：
1kg, fermentation temperature are 35~42 DEG C, and fermentation time is 25~50min.
Compared with prior art, the advantage of the invention is that：
1) fibrous raw material abundance, the cheap pine bark that the present invention selects, not only reduces production cost, but also
Reduce because of the wasting of resources, the environmental pollution caused by discarding, burning these raw materials the problems such as, it is achieved thereby that the utilization of resources is maximum
The purpose of change；
2) present invention is handled by ultrasound wave auxiliary enzyme method, enzymolysis fall in pine bark cellular content and cell membrane albumen and
Soluble polysacchrides material (the wherein pectin enzyme hydrolysis pectic substance of pine bark cell membrane, papain hydrolysis cell membrane
Middle albumen and proteoglycans etc.), discharge albumen and soluble polysacchrides in cellular content and cell membrane and enter in aqueous solution,
Discharged by draining, realize the concentration of dietary fiber, while ultrasonic wave auxiliary is to improve enzymolysis efficiency, reaches energy consumption
Less, the low purpose of enzyme dosage, so as to reduce cost；
3) the lactobacillus plantarum HKB-38 that selects of the present invention be gram-positive bacteria, has that acidproof, growth is vigorous, production acid energy
The features such as power is strong, fermentability is strong；Fermented using lactobacillus plantarum HKB-38, not only to pine bark fermenting raw materials effect more
It is good, and shorten fermentation time, effectively ensure the natural sex and validity of concentration diet fiber product function, go forward side by side one
Walk the physiological properties such as the fermentability for enhancing dietary fiber, retention ability, dilatancy；
4) present invention is heat-treated by high temperature and pressure and opens fibrillation, and lignocellulosic can be at double during high temperature and pressure
Increase absorption water ability, so as to greatly improve the retention ability of diet fiber product, while also reached killing and added plant
The effect of lactobacillus HKB-38；
5) compared with common alkaline process prepares dietary cellulosic in commercialization at present, at lye
The loss of dietary cellulosic caused by reason and the destruction of dietary fiber physiological property, while also further reduce because of a large amount of discharges
Alkaline waste liquor and cause serious problem of environmental pollution.
It is demonstrated experimentally that crude fiber content >=60% in natural concentration diet fiber product produced by the present invention, neutral detergent fiber
(NDF) content >=86%, acid detergent fiber (ADF) content >=70%, product swellability >=5.0mL/g, retention ability >=5.0g/g.
Brief description of the drawings
By reading the detailed description of hereafter preferred embodiment, it is various other the advantages of and benefit it is common for this area
Technical staff will be clear understanding.Attached drawing is only used for showing the purpose of preferred embodiment, and is not considered as to the present invention
Limitation.And in whole attached drawing, identical component is denoted by the same reference numerals.In the accompanying drawings：
Fig. 1 is lactobacillus plantarum HKB-38 microscopes (1000X) of the present invention observation figure.
Fig. 2 is the flat-plate bacterial colony aspect graph of lactobacillus plantarum HKB-38 of the present invention.
Fig. 3 is lactobacillus plantarum HKB-38 systematic evolution trees of the present invention.
Embodiment
The illustrative embodiments of the disclosure are more fully described below with reference to accompanying drawings.Although this public affairs is shown in attached drawing
The illustrative embodiments opened, it being understood, however, that may be realized in various forms the disclosure without the reality that should be illustrated here
The mode of applying is limited.Conversely, there is provided these embodiments are to be able to be best understood from the disclosure, and can be by this public affairs
The scope opened completely is communicated to those skilled in the art.
Embodiment 1：The separation and identification of one lactobacillus plantarum
1st, sodium selenite small intestine (such as duodenum, jejunum, ileum, caecum, colon) intestinal mucosa 5cm is taken2, it is placed in sterilizing and burns
In cup, the content on mucous membrane is rinsed with sterile saline, mucous membrane is immersed in the beaker equipped with 15mL HEPES buffer solutions, is used
After tweezers waggle mucous membrane 5min, the Hungates of Rogosa SL agar is incorporated with asepsis injector absorption 0.2mL supernatants
In rolling tube, gently being rolled with hand makes supernatant be evenly distributed on agar surface in pipe, is put into 5%CO2Incubator, 37 DEG C of cultures
After 72h, the sterile working in Biohazard Safety Equipment, with the bacterium colony of syringe needle picking white needle point size to the sterile Rogosa meat soups of anaerobism
Inside cultivated, whether observation meat soup becomes cloudy, and the 4 DEG C of refrigerators of placement for choose well-grown, having muddiness are stored spare.
2nd, gram-positive bacteria is accredited as by Gram's staining, then by catalase test, is initially identified as Lactobacillus.
3rd, acid resistance selection and breeding：Stomach lactobacillus broth culture medium is respectively adopted and intestine lactobacillus broth bouillon is trained
Support, observation period growing state, select the most luxuriant bacterium of survival rate highest, growth, that is, obtained one plant of bacterium, numbering HKB-38.
4th, finally by the form, physio-biochemical characteristics, 16S of China typical culture collection center identification bacterial strain HKB-38
The measure of rRNA gene orders, specific testing result are as follows：
Bacterial strain HKB-38, micro- sem observation：In elongated rod-shaped, both ends blunt circle, Dan Sheng, no gemma, atrichia, but can transport
It is dynamic, such as Fig. 1；Tablet is observed：Grown on MRS agar mediums, bacterium colony is creamy white, regular circle shapes, center projections, surface light
Sliding moistening, such as Fig. 2.
Bacterial strain HKB-38 physio-biochemical characteristics testing results, such as table 1, table 2.
1 bacterial strain HKB-38 physio-biochemical characteristics of table-enzyme activity, carbon source oxidation
+：Positive reaction；-：Negative reaction；W：Weakly positive is reacted
2 bacterial strain HKB-38 physio-biochemical characteristics of table-utilize carbon source production acid
+：Positive reaction；-：Negative reaction；W- weakly positives are reacted
Through sequencing, bacterial strain HKB-38 16S rRNA gene orders, such as SEQ ID NO：Shown in 1, and bacterial strain HKB-38 16S
RRNA gene sequencing BLAST the results shows and the Lactobacillus in lactobacillus plantarum category in Bank gene pools
The 16S rRNA sequence homology degree highests of plantarum subsp.plantarum, homology is up to 99.59%.It is soft using correlation
Part drawing system chadogram, sees Fig. 3.From the systematic evolution tree of bacterium 16S rRNA structures, bacterial strain HKB-38 and plant breast
Bacillus (Lactobacillus plantarum) development relation is nearest, divides in same monoid, sequence homology 100%.Cause
This, based on systematic evolution tree and 16S rRNA homogeneous angulars, it is one during lactobacillus plantarum belongs to new to determine bacterial strain HKB-38
Member.To sum up, colony characteristics, physio-biochemical characteristics, 16S rRNA gene orders are as a result, be accredited as lactobacillus plantarum
(Lactobacillus plantarum), Classification And Nomenclature are lactobacillus plantarum (Lactobacillus plantarum) HKB-38.
Embodiment 2：Influence of the different pine bark raw materials to natural concentration dietary fiber
1) after Pissodss sp. on Yunnan Pine, bark of dahurian larch, Masson Pine Bark being carried out cleaning removal of impurities respectively, 20 mesh sieves are crushed,
The immersion of room temperature clear water is added, the ratio of raw material and room temperature clear water after crushing is 1g：10mL, soaking time 4h, exhausts waste water.
2) three kinds of pine bark raw materials obtained by step 1) are transferred in reactor respectively, add clear water again, pine bark is former
The ratio of material and room temperature clear water is 1g：20mL, carries out ultrasound-assisted enzymolysis, after it is specially ultrasonic pretreatment 5min, every liter
The pectase that 1g enzyme activities are 1.0U/mg is added in mixed liquor, and (product identification that Sigma Co., USA sells is 17389 fruit
Glue enzyme) and papain that 5g enzyme activities are 12U/mg (product identification that Sigma Co., USA sells is 76218 pawpaw
Protease) digested, hydrolysis temperature is 42 DEG C, and enzymolysis pH value is 6.5, enzymolysis time 30min, draining liq after enzymolysis,
Material after being digested.
3) it is respectively connected to lactobacillus plantarum HKB-38 into the material after three kinds of enzymolysis obtained by step 2) to ferment, plant breast
The mass ratio of bacillus HKB-38 and material is 1g：1kg, fermentation temperature are 37 DEG C, fermentation time 25min, the material after being fermented.
4) high temperature and pressure heat treatment is carried out respectively to the material after three kinds of fermentations obtained by step 3), temperature is 350 DEG C, pressure
For 10MPa, processing time 20min, cooling, drying, obtain three kinds of natural concentration dietary fibers, detect indices, as a result
It is shown in Table 3.
Influence of the different pine bark raw materials of table 3 to natural concentration diet fiber product
As shown in Table 3, under the conditions of identical preparation method, Pissodss sp. on Yunnan Pine, bark of dahurian larch and Masson Pine Bark difference
Crude fiber content is all higher than 60%, NDF contents to be above 86%, ADF contents equal in the natural concentration diet fiber product prepared
Higher than 70%, product swellability is above 5.0mL/g, and retention ability is above 5.0g/g；And Pissodss sp. on Yunnan Pine preparation is natural dense
Contracting dietary fiber index parameter is optimal, can be used as preferred feedstock.
Embodiment 3：Influence of the different probiotics fermentions to natural concentration dietary fiber
1) after Pissodss sp. on Yunnan Pine being carried out cleaning removal of impurities, 30 mesh sieves are crushed, add the immersion of room temperature clear water, the original after crushing
The ratio of material and room temperature clear water is 1g：5mL, soaking time 2h, exhausts waste water.
2) pine bark raw material obtained by step 1) is transferred in reactor, adds clear water, pine bark raw material and room temperature again
The ratio of clear water is 1g：10mL, carries out ultrasound-assisted enzymolysis, after it is specially ultrasonic pretreatment 10min, is added in every liter of mixed liquor
The pectase (product identification that Sigma Co., USA sells is 17389 pectase) and 6g enzyme activity that 1.2g enzyme activities are 1.0U/mg
The papain (product identification that Sigma Co., USA sells is 76218 papain) that power is 12U/mg is digested,
Hydrolysis temperature is 55 DEG C, and enzymolysis pH value is 6.0, enzymolysis time 20min, draining liq after enzymolysis, the material after being digested.
3) lactobacillus plantarum HKB-38, lactobacillus bulgaricus bacterium are respectively connected into the material after enzymolysis obtained by step 2)
Powder (being purchased from Nanjing Bian Zhen bio tech ltd, product identification BZW23003, bacterium number is CICC6032) or thermophilic lactic hammer
Bacterium bacterium powder (being purchased from Nanjing Bian Zhen bio tech ltd, product identification BZW23104, bacterium number is AS1.1864) is fermented, benefit
The mass ratio of raw bacterium and material is 2g：1kg, fermentation temperature are 40 DEG C, fermentation time 30min, the material after being fermented.
4) high temperature and pressure heat treatment is carried out respectively to the material after fermentation obtained by step 3), temperature is 400 DEG C, and pressure is
5MPa, processing time 30min, cooling, drying, obtain three kinds of natural concentration dietary fibers, detect indices, the results are shown in Table 4.
Influence of the different probiotics of table 4 to natural concentration diet fiber product
As shown in Table 4, using identical pine bark as raw material, under other preparation process the same terms, different probiotics fermentions pair
The crude fibre of natural concentration dietary fiber, NDF and ADF contents have little to no effect, but swellability and retention ability shadow to fiber
Significant difference is rung, the swellability of natural concentration dietary fiber and retention ability are significantly higher than after wherein lactobacillus plantarum HKB-38 fermentations
Lactobacillus bulgaricus and Streptomyces thermophilus.
Embodiment 4：Influence of the high temperature and pressure heat treatment to natural concentration diet fiber product retention ability
1) after Pissodss sp. on Yunnan Pine being carried out cleaning removal of impurities, 60 mesh sieves are crushed, add the immersion of room temperature clear water, the original after crushing
The ratio of material and room temperature clear water is 1g：20mL, soaking time 10h, exhausts waste water.
2) pine bark raw material obtained by step 1) is transferred in reactor, adds clear water, pine bark raw material and room temperature again
The ratio of clear water is 1g：30mL, carries out ultrasound-assisted enzymolysis, after it is specially ultrasonic pretreatment 10min, in every liter of mixed liquor plus
Enter the pectase (product identification that Sigma Co., USA sells is 17389 pectase) and 10g enzyme activity that 2g enzyme activities are 1.0U/mg
The papain (product identification that Sigma Co., USA sells is 76218 papain) that power is 12U/mg is digested,
Hydrolysis temperature is 35 DEG C, and enzymolysis pH value is 8.0, enzymolysis time 60min, draining liq after enzymolysis, the material after being digested.
3) into the material after enzymolysis obtained by step 2), access lactobacillus plantarum HKB-38 ferments, lactobacillus plantarum
The mass ratio of HKB-38 and material is 3g：1kg, fermentation temperature are 42 DEG C, fermentation time 50min, the material after being fermented.
4) it is divided into two equal portions to the material after fermentation obtained by step 3), a copy of it carries out high temperature and pressure heat treatment, temperature
For 360 DEG C, pressure 30MPa, processing time 60min, cool down, be dried to obtain natural concentration dietary fiber；Another without
High temperature and pressure heat treatment convection drying obtains naturally concentrating dietary fiber, detects indices, the results are shown in Table 5.
Influence of the different probiotics of table 5 to natural concentration diet fiber product
As shown in Table 5, under the conditions of same materials, identical preparation method, if high temperature and pressure heat treatment is to natural concentration
Crude fibre, NDF and ADF contents and the swelling ability of dietary fiber have little to no effect, but after high temperature and pressure is heat-treated
Considerably improve the retention ability of natural concentration dietary fiber.
As it can be seen that the ultrasound wave auxiliary enzyme method processing by the present invention, improves enzymolysis efficiency, energy consumption less, enzyme dosage it is low, and
The concentration of dietary fiber has been better achieved；Fermented using lactobacillus plantarum, ferment effect is good, enhances dietary fiber
Fermentability, retention ability, the physiological property such as dilatancy；It is heat-treated by high temperature and pressure, significantly improves diet fiber product
Retention ability；In addition, pine bark low in raw material price, abundance, substantially reduces production cost.
To sum up, crude fiber content >=60%, NDF content in the natural concentration diet fiber product that prepared by the present invention >=
86%, ADF content >=70%, product swellability >=5.0mL/g, retention ability >=5.0g/g.
The foregoing is only a preferred embodiment of the present invention, but protection scope of the present invention be not limited thereto,
Any one skilled in the art the invention discloses technical scope in, the change or replacement that can readily occur in, all should
It is included within the scope of the present invention.Therefore, protection scope of the present invention should be based on the protection scope of the described claims.
SEQUENCE LISTING
<110>Beijing middle peasant Hong Ke Bioisystech Co., Ltd of Agricultural University Of Hunan
<120>A kind of natural concentration dietary fiber and preparation method thereof
<130>
<160> 1
<170> PatentIn version 3.5
<210> 1
<211> 1466
<212> DNA
<213> Lactobacillus plantarum
<400> 1
actggcggcg tgctatacat gcaagtcgaa cgaactctgg tattgattgg tgcttgcatc 60
atgatttaca tttgagtgag tggcgaactg gtgagtaaca cgtgggaaac ctgcccagaa 120
gcgggggata acacctggaa acagatgcta ataccgcata acaacttgga ccgcatggtc 180
cgagcttgaa agatggcttc ggctatcact tttggatggt cccgcggcgt attagctaga 240
tggtggggta acggctcacc atggcaatga tacgtagccg acctgagagg gtaatcggcc 300
acattgggac tgagacacgg cccaaactcc tacgggaggc agcagtaggg aatcttccac 360
aatggacgaa agtctgatgg agcaacgccg cgtgagtgaa gaagggtttc ggctcgtaaa 420
actctgttgt taaagaagaa catatctgag agtaactgtt caggtattga cggtatttaa 480
ccagaaagcc acggctaact acgtgccagc agccgcggta atacgtaggt ggcaagcgtt 540
gtccggattt attgggcgta aagcgagcgc aggcggtttt ttaagtctga tgtgaaagcc 600
ttcggctcaa ccgaagaagt gcatcggaaa ctgggaaact tgagtgcaga agaggacagt 660
ggaactccat gtgtagcggt gaaatgcgta gatatatgga agaacaccag tggcgaaggc 720
ggctgtctgg tctgtaactg acgctgaggc tcgaaagtat gggtagcaaa caggattaga 780
taccctggta gtccataccg taaacgatga atgctaagtg ttggagggtt tccgcccttc 840
agtgctgcag ctaacgcatt aagcattccg cctggggagt acggccgcaa ggctgaaact 900
caaaggaatt gacgggggcc cgcacaagcg gtggagcatg tggtttaatt cgaagctacg 960
cgaagaacct taccaggtct tgacatacta tgcaaatcaa gagattagac gttcccttcg 1020
gggacatgga tacaggtggt gcatggttgt cgtcagctcg tgtcgtgaga tgttgggtta 1080
agtcccgcaa cgagcgcaac ccttattatc agttgccagc attaagttgg gcactctggt 1140
gagactgccg gtgacaaacc ggaggaaggt ggggatgacg tcaaatcatc atgcccctta 1200
tgacctgggc tacacacgtg ctacaatgga tggtacaacg agttgcgaac tcgcgagagt 1260
aagctaatct cttaaagcca ttctcagttc ggattgtagg ctgcaactcg cctacatgaa 1320
gtcggaatcg ctagtaatcg cggatcagca tgccgcggtg aatacgttcc cgggccttgt 1380
acacaccgcc cgtcacacca tgagagtttg taacacccaa agtcggtggg gtaacctttt 1440
aggaaccagc cgctaagtga cagatt 1466
Claims (10)
- A 1. lactobacillus plantarum (Lactobacillus plantarum) HKB-38, it is characterised in that December 8 in 2016 Day is preserved in China typical culture collection center, it is referred to as CCTCC, and deposit number is CCTCC NO:M2016727.
- 2. lactobacillus plantarum HKB-38 according to claim 1, it is characterised in that its 16S rRNA gene order such as SEQ ID NO:Shown in 1.
- 3. a kind of natural concentration dietary fiber, it is characterised in that will be enriched in the pine bark grinding and sieving of dietary cellulosic, add water Immersion, exhausts waste water, then the pine bark after immersion is transferred in reactor after having soaked, add water, obtain pine bark and mix Liquid is closed, carries out ultrasound-assisted enzymolysis processing, draining liq after enzymolysis, then accesses claim 1 by the substrate material after enzymolysis Or the lactobacillus plantarum HKB-38 described in 2 ferments, the substrate material after fermentation is finally subjected to high temperature and pressure heat treatment, it is cold But it is, dry, up to natural concentration dietary fiber.
- 4. natural concentration dietary fiber according to claim 3, it is characterised in that the pine tree rich in dietary cellulosic Skin is Pissodss sp. on Yunnan Pine, bark of dahurian larch or Masson Pine Bark, and the water is room temperature clear water.
- 5. natural concentration dietary fiber according to claim 4, it is characterised in that the pine tree rich in dietary cellulosic Skin is Pissodss sp. on Yunnan Pine.
- A kind of 6. preparation method of natural concentration dietary fiber as described in one of claim 3~5, it is characterised in that including Following steps：1) after the pine bark raw material cleaning removal of impurities that will be enriched in dietary cellulosic, 20~60 mesh sieves are crushed, according to sieving pine bark Ratio with water is 1g：5~20mL, adds water and soaks 2~10h, waste water is exhausted after having soaked；2) pine bark after immersion obtained by step 1) is transferred in reactor, according to the pine bark after immersion and room temperature clear water Than for 1g：10~30mL, adds water, obtains pine bark mixed liquor, carries out ultrasound-assisted enzymolysis processing, liquid is drained after enzymolysis Body, the material after being digested；3) into the material after enzymolysis obtained by step 2), access probiotics is fermented, material fermented after material；4) material after fermentation obtained by step 3) is heat-treated under conditions of temperature is 350~400 DEG C, pressure is 5~30MPa 10~60min, cooling, drying,.
- 7. the preparation method of natural concentration dietary fiber according to claim 6, it is characterised in that described in step 2) Ultrasound-assisted enzymolysis is 2~10min of first ultrasound, then adds enzyme preparation and is digested, hydrolysis temperature is 35~55 DEG C, enzymolysis PH value is 6.0~8.0, and enzymolysis time is 20~60min.
- 8. the preparation method of natural concentration dietary fiber according to claim 7, it is characterised in that the enzyme preparation is fruit Glue enzyme or/and papain；The enzyme activity of the pectase is 0.5~5.0U/mg, pectase additive amount and pine bark mixed liquor Ratio be 1~2g：1L；The enzyme activity of the papain is 5~50U/mg, papain dosage and pine bark mixed liquor Ratio be 5~10g：1L.
- 9. the preparation method of natural concentration dietary fiber according to claim 8, it is characterised in that the enzyme of the pectase Work is 1.0U/mg, and the enzyme activity of the papain is 12U/mg.
- 10. the preparation method of the natural concentration dietary fiber according to one of claim 6~9, it is characterised in that step 3) In, the mass ratio of the lactobacillus plantarum HKB-38 and substrate material after enzymolysis are 1~3g：1kg, fermentation temperature are 35~42 DEG C, Fermentation time is 25~50min.
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| CN102864093A (en) * | 2012-04-18 | 2013-01-09 | 北京和美科盛生物技术有限公司 | Lactobacillus plantarum suitable for silage straw and use method thereof |
| CN105029452A (en) * | 2015-07-06 | 2015-11-11 | 青岛嘉瑞生物技术有限公司 | Method used for extracting dietary fiber from wheat straw via biological method |
| CN105124134A (en) * | 2015-07-21 | 2015-12-09 | 北京中农弘科生物技术有限公司 | Method of preparing feed dietary fibers |
| CN105533134A (en) * | 2015-12-16 | 2016-05-04 | 惠安县科联农业科技有限公司 | Preparation method of fermentation feed |
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| CN102864093A (en) * | 2012-04-18 | 2013-01-09 | 北京和美科盛生物技术有限公司 | Lactobacillus plantarum suitable for silage straw and use method thereof |
| CN105029452A (en) * | 2015-07-06 | 2015-11-11 | 青岛嘉瑞生物技术有限公司 | Method used for extracting dietary fiber from wheat straw via biological method |
| CN105124134A (en) * | 2015-07-21 | 2015-12-09 | 北京中农弘科生物技术有限公司 | Method of preparing feed dietary fibers |
| CN105533134A (en) * | 2015-12-16 | 2016-05-04 | 惠安县科联农业科技有限公司 | Preparation method of fermentation feed |
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| CN109601734A (en) * | 2018-12-28 | 2019-04-12 | 北京中农弘科生物技术有限公司 | Feed addictive containing procyanidine and dietary fiber and preparation method thereof and nonreactive pig starter feed product |
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