CN102212479B - Method for pretreating beer waste for biochemical utilization - Google Patents
Method for pretreating beer waste for biochemical utilization Download PDFInfo
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- CN102212479B CN102212479B CN 201110094439 CN201110094439A CN102212479B CN 102212479 B CN102212479 B CN 102212479B CN 201110094439 CN201110094439 CN 201110094439 CN 201110094439 A CN201110094439 A CN 201110094439A CN 102212479 B CN102212479 B CN 102212479B
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Abstract
The invention provides a method for pretreating beer waste for biochemical utilization. The method comprises the following steps: carrying out ozone wall breaking and sterilization on one or mixture of beer waste water, sludge, waste and fresh beer yeast slurry so as to increase leaching of nutrient substances of yeast content, wherein the ozone admission amount is 0.3-0.8g of ozone/ g of waste beer dried substances; and carrying out high-pressure steam sterilization on samples subjected to ozone treatment at 121 DEG C for 45 minutes, thus the samples subjected to high-pressure steam sterilization can used for microorganism yeast culture. The beer waste pretreated by the method provided by the invention has the advantages that the nutrient substance release rate can be greatly improved, the content of amino nitrogen in a culture medium is improved by 4-5 times, and the amino nitrogen yield achieves 3-5 percent, thus the pretreated beer waste serving as the culture medium is beneficial to microorganism culture.
Description
Technical field
The present invention relates to the pretreatment process of beer ferment waste biological utilisation, belong to the ferment technical field.
Background technology
Ozone (0
3) be oxygen (0
2) allotropic substance, by 3 Sauerstoffatoms, formed, at normal temperatures and pressures for a kind of unstable gas with irritating smell, very easily resolve into oxygen.Ozone, due to its strong oxidizing property, all is widely used in all fields, as food, tap water, sewage disposal sterilization, sludge reduction etc.The advantage of ozonize: (1) can manufacture in production scene, does not need the accumulating link, has reduced operational danger; (2) ozone has strong oxygenizement, and speed of response is fast.But kill vegetative forms of bacteria, gemma, virus, fungi, protozoon packing etc., its inactivating efficacy is than the fast 600-3000 of chlorhexidine-containing disinfectant doubly; (3) ozone disinfection by-product is mainly the organism such as aldehyde, aromatic carboxylic acid.(4) in water, the very fast natural decomposition of remaining ozone is oxygen, contain higher dissolved oxygen in water outlet, and ozone is harmless to healthy cell, is the cleanest sterilizing agent.The method of relevant ozone pre-treatment waste yeast mud has no report.
Ozone sterilization dissolves thalline mechanism: Xu Huaide etc. (2004) ozone can act on cytolemma, make the film constituent impaired, and cause dysbolism, and continue permeates cell membranes and destroy ester gp and lipopolysaccharides in film, change the permeability of cell, cause cytolysis death.Finally cause cellular component to be leaked in intermediate medium, can be proven by the increase of medium amplifying nucleic acid and protein.
To add yeast in beer fermentation process, when yeast utilizes conversion of glucose ethanol, produce a large amount of thalline simultaneously, these thalline are continued on for beer fermentation except a part, major part becomes used yeast slurry, generally all as discharging of waste liquid, accounts for 37% of beer waste water pollution load during discharge, the Retrieve beer yeast can reduce beer waste water pollution load 50% (COD) (Zou Fajun, 2002).Waste yeast mud impurity is many, of poor quality, most of brew-house or waste yeast is directly drained, squeeze into the beer vinasse, with several nominal prices of a jin of sharing money, near peasant allowing makes fertilizer, feed pulls away, and this had both become the another large new source of pollution such as rivers, lake, was again a surprising waste.
The beer waste yeast whole body is all precious, yeast cell water content 75%-85%, dry-matter accounts for the 15%-25% of weight in wet base, cerevisiae contains the multiple nutritional components (Li Xingge etc., 1998) such as rich in protein, nucleic acid, VITAMIN, carbohydrate, lipid material, mineral substance, and the yeast thalline is that any animals and plants are without comparable measure containing the height of protein, dry-matter contains 50% protein, contain 18 seed amino acids etc., also have 8 seed amino acids of needed by human, be rich in synthetic
cryrequired amino acid; Waste yeast is high, and containing nucleic acid, wherein the massfraction of Yeast Nucleic Acid (RNA) is 2.7%-10.0%, and the massfraction of thymus nucleic acid (DNA) is 0.03%-0.516% (Rehm, 1967), contains higher RNA/DNA ratio.Beer waste yeast is rich vitamin B also
1, B
2, B
6, B
12, reach 0.7% of yeast dry weight, and exist mainly with the phosphoric acid ester form, contain vitamins D, E (supervisor's instrument, 1982), wheat sterol, nicotinic acid, folic acid, pantothenic acid, inositol etc.; Contain organic acid, sugar and 14 kinds of mineral substance phosphorus, iron, calcium, sodium, potassium, magnesium etc., and abundant enzyme is proteolytic enzyme, Phospholipid hydrolase, trypsinase and coenzyme A and physiologically active substance, as coenzyme A, ubiquinone, nadide, cytochrome C, thromboplastinum, gsh etc.Extract these bioactive functions materials and receive publicity gradually, add certain bifidus factor if any research in beer yeast slurry, but fermentative production bifid yeast protective foods; Utilize used yeast slurry production nutrition fruit vinegar, fermented acid milk drink.Beer waste yeast can be used for producing yeast extract, nutritional flavouring agent, cell wall polysaccharide, protein powder and aminoacids complex; In pharmaceutical industry, beer waste yeast can be used as starting material and extracts superoxide-dismutase, 1, the materials such as 6-hexose diphosphate, nucleic acid, Triptide, dextran, mannosans, Lupulin and phosphoesterases complex, (Guo Xuexia, 2007) have broad application prospects.But be difficult to the thick cell walls of digestion and high RNA/DNA ratio nucleic acid because the yeast thalline contains mankind's (non-ruminant animal), directly eat and can cause that Uric Acid content is higher, cause gout and urinary stone (Li Xiang, 2001).The developed countries such as Japan in Europe, the United States and Asia, utilize beer yeast slurry, developed yeast extract, nucleic acid, Nucleotide, ergosterol, beta-glucan, trehalose and gsh etc., and many products have formed scale production (Choi etc., 1998; Lee etc., 1993; Matsushita etc., 1999; Sombutyanuchit etc., 2001; Kong Ming etc., 2003), Japan cereuisiae fermentum utilize aspect the prostatitis in the Zou Liao world, wherein the 17%-18% of Retrieve beer waste yeast is for pharmaceutical production, 20% for foodstuff production, 12%-13% is approximately arranged for fortified feed production, and 50% for mixed fodder production.The beer production of China occupies the first in the world for years at present, produces and has formed grouping of the world economy, mass-producing, but utilize the research of waste yeast that certain gap is still arranged with developed country, basically also rests on laboratory or pilot scale level (Sun Weifeng, 2008).Mostly brew-house all only passes through and simply dries as feed the processing of yeast slurry.Waste yeast mud is through dehydrating into dry yeast, dehydrates that technological equipment investment is large, power consumption is large, cost is high, and yeast is limited again as byproduct quantity, lacks the benefit (Gao Lu, 2004, Guo Xuexia, 2007) of scale production.From the eighties in 20th century, just gradually relevant for utilizing waste yeast to prepare the report of yeast extraction, but great majority are still in laboratory stage, only have minority producer to put into production, but small scale, the product category dullness, quality is not high, be difficult to be accepted (Sun Weifeng, 2008) by food service industry, although many about the research report of beer waste yeast comprehensive utilization, but be applied to produce the less (Zuo Yong etc. of actual achievement in research, 2004), with compare in addition a certain distance (Zhu Zhenqi, 1996) abroad.Waste yeast is dried or directly discharge, not further processing (Zuo Yong etc., 2004), and effective constituent is not fully used, and has caused the wasting of resources, has polluted environment.Because waste yeast mud is nutritious, someone studies beer yeast slurry for biological culture, as be used as edible fungus culturing raw material, the substratum of producing fuel ethyl alcohol by ferment, glycerine and biogas.
Further process waste yeast, effective constituent is fully used, and the release of remaining endogenous proteinase starts from the fragmentation of yeast cells wall and the change of membrane permeability, and the degree of crushing of cell walls directly affects its process costs and quality product.The cytoclastic method of research has (1) enzyme autolysis method at present: along with the carrying out of waste yeast self-dissolving, enzyme activity constantly descends, the simple interior enzyme system of yeast body that relies on can not make the yeast cells wall degraded, can not make intracellular macromolecular substance fully degrade, therefore want additional a certain amount of enzyme or promotor to promote yeast autolysis, as improved from solubility temperature; Add soda acid; Add promotor to change cell permeability, the Putrefaction of anti-bacteria as plasmolyticum sodium-chlor, chloroform, sucrose, vinyl acetic monomer, toluene, amyl acetate-n, Repone K, ethanol, halfcystine (Zhao Mouming, 2001) etc.Add different chemical substance treatment viable bacteria bodies, the destruction that can cause some nutritive ingredients, cause protein denaturation or degraded, and add to the difficulties for the extraction of effective constituent, makes the protein recovery rate reduce.Also having better method is exactly additional single or prozyme promotion self-dissolving.The degree that Shen Guohui (1991) report yeast cell extracts depends primarily on temperature, pH, time and yeast type, interpolation salt or proteolytic enzyme can significantly improve the yield of yeast extract, and in additional enzyme preparation hydrolysed leaven cell walls or born of the same parents, macromole improves the yeast autolysis yield.Lee(1993), Potman(1969) also report enzymolysis process reaction conditions gentleness; broken wall is more thorough; sporoderm-broken rate is significantly higher than other wall-breaking methods; situation at developed country's plus enzyme and autolysis method Integrated using is more, and acid-alkali treatment method is because the aftertreatment cost is high, pollution level substantially no longer adopts greatly now; But enzyme process also can cause the cell autodigestion, protein degradation, the rate of recovery is low.Ten thousand duckweeds (2000) are reported because the speed of response of enzyme is fast. and need aseptic technique, operation is controlled also cumbersome, and large-scale the employing is restricted, and in the product that interpolation salt is gathered in the crops, contains salt, and range of application has limitation.Therefore, plus enzyme depends on industrial enzyme preparation kind, performance and the application level of desirable proteins enzyme.The existing special-purpose commercial enzyme of Japan is for yeast extract production; The domestic source that is limited to enzyme and to the consideration of enzyme-added rear production cost, the research of this respect less (Tao Xingwu, 2004).The yeast extract production level of China is lower, is mainly manifested in that the product yield is low, amino nitrogen content is low, color and luster is partially dark, local flavor formulation single (Zhong Ruimin, 2004).(2) high pressure homogenization and high-pressure compressing method: this method has been eliminated the sterically hindered of substrate and enzyme, thereby accelerate the self-dissolving of yeast cell, high-pressure homogeneously in addition also can destroy most of yeast cells wall, be conducive to generate the micromolecular stripping of small-molecule substance and yeast cell itself.Although this method is simple, cost is low, the intact preservation nutritive ingredient of energy, its shell-broken effect is poor, under certain pressure, a homogeneous can only make a part of cytoclasis of yeast suspension basically, if will make more cell wall breaking, will repeatedly repeat fragmentation.(3) supersonic wave wall breaking and microwave broken wall: using the advantage of supersonic wave wall breaking is, when due to him, improving the cell permeability, not injure cell itself.Ultrasonicly as a kind of energy field, add in fermented liquid, can not increase the organic contamination of fermented liquid, later separation is had to very important meaning.Ultrasonic fragmentation has the characteristics such as save time, efficient, easy, loss of liquid is few; (4) pulsed electric field (HPEF) broken wall: adopt HPEF and stir in conjunction with carrying out the cell wall breaking extraction, can extract to a great extent cellular material, and avoid introducing extra chemical substance, simplified subsequent treatment process.(5) other: as pyrolysis method (with 100 ℃ of water boil yeast, with plasmolysis method (processing with high permeating sodium chloride solution at the temperature far below 100 ℃) (Wang Jiaohong, 2001).(Zheng Jianxian compiling, 1991) report that the yeast suspension liquid temp being risen to gradually in 30-l50min to the cultivation temperature also can improve self-dissolving speed, but it is limited to improve the heat-treated waste yeast, it can make autolytic enzyme whole inactivations basically, simultaneously, intracellular macromolecular substance continues hydrolysis (Zhou Deqing, 2002) under the effect of water and heating power.Report CO out from the micropore gas distribution piping is separately arranged
2gas has very high pressure, when the yeast cell in solution touches these high pressure bubbles, and high pressure CO
2meeting instantaneous infiltration yeast cell inside, and continue to expand, burst cell walls (Bai Bing, 2005).Above method can be used alone or ties platform and get up to use.
Pre-treatment before above-mentioned used yeast slurry utilizes, cost is higher, technique is more complicated, and after processing, the nutrition release rate of yeast is not high, the processing of beer waste water, mud also has similar situation, therefore people all find a kind of clean, convenient, efficiently, treatment process cheaply, for its biological recycling provides support.
Summary of the invention
The purpose of this invention is to provide a kind of novel, clean, efficiently, the pretreatment process of beer by-products biological utilisation cheaply, to improve the bioavailability of beer by-products.
The objective of the invention is to realize as follows:
The pretreatment process that a kind of beer waste for biochemical utilizes, comprise beer waste water, mud, useless fresh beer yeast slurry separately or the ozone broken wall of mixture, disinfect, to increase the leaching of thalline content nutritive substance, alleviate the later stage sterilizing works.The dry-matter that the ozone intake is 0.3-0.8g ozone/g beer by-products; Sample after processing, in 121 ℃ of high pressure steam sterilization 45min, can be used for the fermentation culture of microorganism.
Described beer waste water, directly pass into the ozone that ozonizer produces, and the ozone intake is 0.3-0.5g ozone/g waste water dry-matter; Sample after processing is in 121 ℃ of high pressure steam sterilization 20-45min; The fermentation culture that can be used for microorganism through above-mentioned pre-treatment.
Described useless fresh beer yeast slurry or the pretreatment process of mud or its mixture are that the ozone intake is 0.5-0.8g ozone/g beer yeast slurry or mud or its mixture dry-matter; Sample after ozonize, in 121 ℃ of high pressure steam sterilization 45min, can be used for the fermentation culture of microorganism.
Described useless fresh beer yeast slurry or mud are with tap water dilution 3-6 doubly, then directly pass into the ozone that ozonizer produces, the ozone intake is 0.3g-0.8 ozone/g beer yeast slurry or mud dry-matter, and the sample after processing is in 121 ℃ of high pressure steam sterilization 45min; The fermentation culture that can be used for microorganism through above-mentioned pre-treatment.
Above-mentioned useless fresh beer yeast slurry or sludge pre-treatment method, with beer waste water dilution 3-6 doubly, pass into the ozone that ozonizer produces, the ozone intake is 0.5g-0.8 ozone/g beer yeast slurry or mud and beer waste water mixture dry-matter, and the sample after processing is in 121 ℃ of high pressure steam sterilization 45min; The fermentation culture that can be used for microorganism through above-mentioned pre-treatment.
Above-mentioned useless fresh beer yeast slurry and mud are mixed, add beer waste water dilution 3-6 doubly, pass into the ozone that ozonizer produces, the ozone intake is 0.5g-0.8 ozone/g beer yeast slurry, mud and beer waste water mixture dry-matter, and the sample after processing is in 121 ℃ of high pressure steam sterilization 45min; The fermentation culture that can be used for microorganism through above-mentioned pre-treatment.
After above-mentioned pre-treatment, the amino nitrogen content of substratum is than improving 4-5 before processing doubly, the fresh beer yeast slurry given up of take is example with tap water dilution 3-6 substratum doubly, its amino nitrogen can be increased to 500-800 mg/L from 100-200 mg/L, through 121 ℃ of high pressure steam sterilization 45min, the yeast viable bacteria is cultivated as 0cfu/mL; The amino nitrogen recovery rate is: 3-5%(yeast juice amino nitrogen content g/ yeast dry weight g*100%).
From principle, analyze, the pre-treatment of employing aforesaid method has changed the membrane permeability of thalline, the stripping that can improve entocyte.Same supersonic wave wall breaking, microwave broken wall, HPEF broken wall compare, O
3be easy to get, add in fermented liquid " disappearance " soon to become nontoxic volatile O
2increase and supersonic wave wall breaking, microwave extraction method are relatively, can above extract largely cellular material, there is supersonic wave wall breaking, microwave broken wall, HPEF extracting method: avoid introducing extra chemical substance simultaneously, can not increase the organic contamination of fermented liquid, simplify subsequent treatment process, there are the characteristics such as save time, efficient, easy, loss of liquid is few.In addition, supersonic wave wall breaking, microwave broken wall, pulsed electric field are difficult to industrialization, and ozone has been widely used in the sterilization of food and sewage disposal.Find that ozone can improve amino nitrogen greatly, and relatively little on the reducing sugar impact, and can increase the dissolved oxygen of substratum, and add chemical substance or enzyme relatively, not only cost-saving but also clean, amino nitrogen, reducing sugar extraction yield are high; Facts have proved that ozone pre-treatment nutrient solution cultivation Bt bacterium power strengthens.
Specific embodiment
example 1
The 500mL triangular flask adds respectively the pending sample of 200mL, ultrasonic generator: the Nanjing sunrise boat KQ-250E of scientific instrument company limited ultrasonic cleaner; Microwave generator: the MK-2270EG of Haier (VC); Ozonizer requires per hour to produce ozone 25g.Bentonite, gac adopt foodstuff production to filter the product of use.
Employing table 1 diverse ways brewery wastewater treatment is cultivated its result:
Beer waste water is raw material, with FeSO
47H
2more than the sample amino nitrogen that O, ozonize mode are processed reaches 28.5 mg/L, than having improved 73% before untreated; But FeSO
47H
2o processes and has added metal ion, and this concentration of metal ions is too high bad; Adopt Activated Carbon Pretreatment, because of adsorption, after sterilizing, amino nitrogen is too low.The ozonize mode is more satisfactory, and result is as table 1.
table 1 beer waste water different pretreatments result
Medium sterilization is detection method as a result: 121 ℃ of sterilizing 30min of malt extract medium, the 1mL sample is drawn in aseptic technique, coats on sterilized solid culture primary surface (90mm), cultivates 48h for 28 ℃, counting.
Adopt table 2 diverse ways to process beer yeast slurry: to contain dry yeast 21.1% beer fresh yeast mud, it is raw material that tap water dilutes 3 times, relatively ozone, ultrasonic wave, H
2o
2, microwave+H
2o
2, the different disposal methods yeast slurry of microwave: the yeast slurry sample with three times of tap water dilutions is done contrast.The sample of ultrasonication and ozonize is best, and its amino nitrogen has improved respectively 80.1%, 73.2%, and adopts the amino nitrogen of microwave treatment to reduce on the contrary 40%; Though adopt the ultrasonication mode can greatly improve amino nitrogen, but its impact on reducing sugar is larger, adopt ozonize to yeast slurry stoste, the raising all favourable (the amino nitrogen recovery rate is 4.5%) of diluting the yeast slurry amino nitrogen of three times, the damage of reducing sugar is minimum.Ozonize is described, fairly obvious advantage is arranged, result is as table 2.
table 2 yeast slurry different pretreatments result
specific embodiment 2
To contain dry yeast 21.1% beer fresh yeast mud, beer raw wastewater, ozonizer, fermentor tank, 8010 bacterial strains;
Adopt following three kinds of culture medium culturing: (1) without the beer waste water of ozonize, 121 ℃ of high pressure steam sterilization 45min.(2) beer waste water, directly pass into the ozone that ozonizer produces, and the ozone intake is controlled and is: treatment time 2min, passing into total amount is 0.5g ozone/g yeast slurry dry-matter, 121 ℃ of high pressure steam sterilization 45min.The fresh waste yeast mud of (3) 6 times of dilutions directly passes into the ozone that ozonizer produces, and the ozone intake is controlled and is: treatment time 2min, passing into total amount is 0.5g ozone/g yeast slurry dry-matter, 121 ℃ of high pressure steam sterilization 45min.Pretreated yeast slurry mixes and forms substratum by 1:5 with (2) pretreated beer waste water.
Fermented liquid brood cell method of counting: get 85 ℃ of heat treated 5min of fermented liquid, cooling rapidly, adopt dilution plate viable bacteria technical process counting;
Fermented liquid toxicity test method: Plantula Brassicae chinensis is soaked in 1000 times of fermented liquid dilutions, dries and is cut into equirotal small pieces in culture dish, and each culture dish is put the small cabbage moth in 10 2-3 ages, three repetitions, and 24h, 48 h observe.
Fermentation culture method: the 10L canned 6L substratum that ferments, adjusting Medium's PH Value with sodium hydroxide is 7.5, the seed liquor 5% that the inoculation activation is spent the night, described bacterial classification contains bacillus thuringiensis 8010 bacterial strains of 10% glycerine-20 ℃ preservation for this chamber; If the shaker fermentation temperature is 30 ± 1 ℃, rotating speed 400r/min, ventilation 1.2m
3/ h, cultivate 25-30h, to 95% bacillus exfoliation fermentation ends.Fermentation ends secondary fermentation liquid carries out the gemma counting and does the small cabbage moth biological assay.
experimental result
(1) after above-mentioned various medium sterilization, microorganism detection is 0cfu/mL, and wherein the amino nitrogen of the 6 waste yeast mud that are diluted reaches 620.76mg/L(and is converted to 3 times of dilutions), the amino nitrogen recovery rate is 4.35%.
(2) above-mentioned three kinds of different culture medias are cultivated, and brood cell's quantity variance of generation is obvious.Beer waste water culture medium culturing 8010 after ozonize, fermentation ends brood cell's number reaches 1.64 * 10
9cfu/mL.Ozonize beer waste water+yeast slurry fermentation culture 8010, fermentation ends brood cell's number can reach 2.08 * 10
9cfu/mL
.by biological assay, find that 24h and 48h small cabbage moth give birth to survey, after ozonize, culture medium culturing 8010 fermented liquid insecticidal effects are better.
the virulence that table 3 8010 bacterial strains are cultivated with different waste water/culturing sludge relatively
Annotate: Ck is beer acidified water after ozonize.
Claims (5)
1. the pretreatment process that a beer waste for biochemical utilizes, comprise beer waste water, mud, useless fresh beer yeast slurry separately or microorganism broken wall and the sterilizing of mixture, it is characterized in that adopting ozone broken wall, sterilization, the dry-matter that the ozone intake is 0.3-0.8g ozone/g beer by-products, sample after ozonize, in 121 ℃ of high pressure steam sterilization 45min, can be used for the fermentation culture of microorganism.
2. the pretreatment process that a kind of beer waste for biochemical according to claim 1 utilizes, is characterized in that beer waste water directly passes into the ozone that ozonizer produces, and the ozone intake is 0.3-0.5g ozone/g beer waste water dry-matter; Sample after ozonize is in 121 ℃ of high pressure steam sterilization 45min; The fermentation culture that can be used for microorganism.
3. the pretreatment process that a kind of beer waste for biochemical according to claim 1 utilizes, is characterized in that beer yeast slurry or mud or its mixture ozone intake are 0.5-0.8g ozone/g beer yeast slurry or mud or its mixture dry-matter; Sample after ozonize, in 121 ℃ of high pressure steam sterilization 45min, can be used for the fermentation culture of microorganism.
4. the pretreatment process utilized according to the described a kind of beer waste for biochemical of claim 1 or 3, it is characterized in that beer yeast slurry or mud are with tap water dilution 3-6 doubly, then directly pass into the ozone that ozonizer produces, the ozone intake is 0.3g-0.5 ozone/g beer yeast slurry or mud dry-matter, sample after ozonize, in 121 ℃ of high pressure steam sterilization 45min, can be used for the fermentation culture of microorganism.
5. the pretreatment process utilized according to the described a kind of beer waste for biochemical of claim 1 or 3, it is characterized in that beer yeast slurry or mud are with beer waste water dilution 3-6 doubly, pass into the ozone that ozonizer produces, the ozone intake is 0.3g-0.5 ozone/g beer yeast slurry or mud and beer waste water mixture dry-matter, and the sample after ozonize can be used for the fermentation culture of microorganism in 121 ℃ of high pressure steam sterilization 45min.
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| CN103160439A (en) * | 2011-12-14 | 2013-06-19 | 新奥科技发展有限公司 | Microalgal wall-breaking method |
| CN104529596A (en) * | 2015-01-16 | 2015-04-22 | 南开大学 | Method for producing organic fertilizer by use of pretreated sludge |
| CN104893976A (en) * | 2015-03-31 | 2015-09-09 | 台州艾希尔生物科技有限公司 | Method for producing aerobic type single-cell protein through autolysis process |
| CN105176850A (en) * | 2015-03-31 | 2015-12-23 | 台州艾希尔生物科技有限公司 | Method for production of aerobic single-cell protein by enzymolysis tank autolysis process |
| CN105166322A (en) * | 2015-03-31 | 2015-12-23 | 台州艾希尔生物科技有限公司 | Method for high yield production of aerobic single-cell protein by autolysis process |
| CN109652342B (en) * | 2019-02-01 | 2022-06-03 | 福建农林大学 | Method for preparing Bt liquid fermentation culture medium by using vegetable wastes as raw materials |
| CN110699292B (en) * | 2019-10-31 | 2022-09-02 | 巢湖学院 | Probiotic culture method based on agricultural and livestock product waste |
| CN114292133A (en) * | 2022-01-26 | 2022-04-08 | 合肥学院 | Process for preparing organic fertilizer by utilizing black beer lees compost fermentation |
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