CN101659970A - Method for circularly treating avermectins waste ferment water and pleurin waste ferment water - Google Patents
Method for circularly treating avermectins waste ferment water and pleurin waste ferment water Download PDFInfo
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Abstract
The invention discloses a method for circularly treating avermectins waste ferment water and pleurin waste ferment water, belonging to the technical field of methods for treating antibiotics waste ferment water. The content of the method comprises: the waste ferment water generated by producing avermectins is used for fermenting and producing pleurin; and the waste ferment water generated by producing pleurin is used for producing avermectins. The method can prevent avermectins waste ferment water and pleurin waste ferment water from being discharged at will, thus achieving environmental-friendly effect, simultaneously leading the two waste water to be repeatedly used and have high utilization value; and the experiment shows that the titer of the product obtained by fermenting the two treated waste water is equivalent to that of the product obtained by fermenting natural water, thus further lowering the enterprises production cost by 60-70 percent compared with the traditional method,and saving environmental-friendly investment and treating expense in the aspect of waste water treating.
Description
Technical field
The invention belongs to the technical field of the treatment process of antibiotic fermentation waste water, be specifically related to abamectin fermented waste water and pleurin fermentation waste water circulation process method.
Background technology
Avrmectin is a kind of agricultural antibiotic, and it promptly can also can be used for Veterinary Insecticides as agricultural insecticide.Agricultural, forestry desinsection and animal expelling parasite worldwide have been widely used in.China existing nearly 20 tame enterprises produce Avrmectin, produce closely kiloton of Avrmectin per year.Avrmectin is through liquid fermenting production by A Fuman streptomycete Streptomyces avermitilis, present stage Avrmectin the average fermentation level about 4000ug/ml, with regard to this rough calculation, 1 ton of Avrmectin of every production (Avm) produces 250 tons fermentation waste water approximately, and the fermentation waste water generation of producing 1000 tons of Avm so per year reaches 250,000 tons.These waste water were not mostly handled and just were discharged in the environment domestic present stage, and pass through anaerobism/oxygen consumption or the secondary oxygen consumption that have are handled the back qualified discharge.
The cutting back pleurin is the intermediate that is used for the safe second rhzomorph of microbiotic fumaric acid synthetic for animals.It is to be produced through liquid fermenting by a kind of oblique top bacterium (Clitopus-pinsitus).China from 2002 from this bacterial classification of external introducing, had 5 tame enterprises to begin the fermentative production pleurin in succession from 2004.Present stage nearly 500 tons of output.Annual production will be crossed thousand after adding producer on new and expansion factory of old factory.1 ton of pleurin of every production, its fermentation waste water generation just reaches 120 tons, calculates in view of the above, produces its wastewater discharge of kiloton pleurin per year and will reach 120,000 tons.
This shows that abamectin fermented waste water that domestic ubiquity is a large amount of and pleurin fermentation waste water arbitrarily discharge, do not have the method for relevant recycling, certainly will cause the influence of environment like this, be unfavorable for environmental protection, caused the waste of resource simultaneously.
Summary of the invention
Problem at the prior art existence, it is a kind of actinomycetes with bacterial classification that the present invention utilizes abamectin fermented, pleurin fermentation is a basidiomycetes in a kind of thread higher fungi with bacterial classification, cultivate the bacterial classification of abamectin fermented usefulness with the waste water that the pleurin fermentation produces, its growth and synthetic Avrmectin are not affected, cultivate the bacterial classification of pleurin fermentation usefulness with the waste water of abamectin fermented generation, its growth and synthetic pleurin are not affected, and mostly produce this phenomenon of pleurin in the enterprise that produces Avrmectin, design provides a kind of environmental protection, the abamectin fermented waste water that water reuse value height and processing cost are low and the technical scheme of pleurin fermentation waste water circulation process method.
Described abamectin fermented waste water and pleurin fermentation waste water circulation process method is characterized in that being used for the fermentative production pleurin with producing the fermentation waste water that Avrmectin produces, and with producing the fermentation waste water that pleurin produces, are used for the fermentative production Avrmectin;
The described fermentation waste water that will produce the Avrmectin generation, be used for the fermentative production pleurin and comprise following processing step: the abamectin fermented liquid that 1) will ferment 265-280 hour carries out press filtration, obtain mycelia filter cake and filtrate, the filtrate of gained is abamectin fermented waste water, 2) PH to 10-12 of the abamectin fermented waste water of accent step 1) gained, be warming up to 70-80 ℃ then, be incubated after 20-30 minute, cool off, filter, 3) with step 2) filtrate of gained squeezes in the fermentor tank, and accent filtrate PH to 7.0-7.5, add to cultivate the nutritive ingredient of oblique top bacterium then, 117 ℃ of sterilizations 30 minutes, be cooled to 26 ℃ after, inoculation oblique top bacteria liquid seed was cultivated 280-300 hour down at temperature 25-26 ℃;
The described fermentation waste water that will produce the pleurin generation, be used for the fermentative production Avrmectin and comprise following processing step: the pleurin fermented liquid that 1) will ferment 280-300 hour carries out press filtration, obtain mycelia filter cake and filtrate, the filtrate of gained is the pleurin fermentation waste water, 2) PH to 10-12 of the pleurin fermentation waste water of accent step 1) gained, be warming up to 70-80 ℃ then, be incubated after 20-30 minute, cool off, filter, 3) with step 2) filtrate of gained squeezes in the fermentor tank, and accent filtrate PH to 7.0-7.5, add to cultivate the nutritive ingredient of A Fuman streptomycete then, 121 ℃ of sterilizations 30 minutes, be cooled to 28 ℃ after, inoculation A Fuman streptomycete liquid seeds was cultivated 256-280 hour down for 28 ℃ in temperature.
Described abamectin fermented waste water and pleurin fermentation waste water circulation process method, it is characterized in that and to produce the fermentation waste water that Avrmectin produces, the step 1) that is used for the fermentative production pleurin is gone into abamectin fermented liquid pump in autoclave diaphragm half frame, at 6-12 kilogram/cm
2Pressure under carry out press filtration.
Described abamectin fermented waste water and pleurin fermentation waste water circulation process method, it is characterized in that and to produce the fermentation waste water that Avrmectin produces, be used for the interpolation of nutritive ingredient of the step 3) oblique top bacterium of fermentative production pleurin: the weight by filtrate adds corn steep liquor 5%, soybean cake powder 1%, stream adds glucose in culturing process, and the level of keeping fermented liquid glucose is at 0.5-2%.
Described abamectin fermented waste water and pleurin fermentation waste water circulation process method, it is characterized in that and to produce the fermentation waste water that Avrmectin produces, after being used for the step 3) accent filtrate PH to 7.0-7.5 of fermentative production pleurin, weight by filtrate adds glucose 0.5-1.5%, ammonium sulfate 0.5-1.0%, potassium primary phosphate 0.015-0.02%, dipotassium hydrogen phosphate 0.0001-0.001%, the sal epsom 0.02-0.08% of 7 water, the U-Ramin MC 0.01-0.05% of sodium-chlor 0.01%-0.05% and 2 water, 117 ℃ of sterilizations are after 30 minutes, be cooled to 28 ℃, in above-mentioned filtrate, inoculate the yeast starter liquid of filtrate weight 10-40% again, after 28 ℃ of temperature are cultivated 48-72 hour down, get yeast culture liquid, regulate the PH to 7.0-7.5 of yeast culture liquid, add the nutritive ingredient of cultivating the oblique top bacterium then, sterilized 30 minutes for 117 ℃, after being cooled to 26 ℃, inoculation oblique top bacteria liquid seed was cultivated 280-300 hour down at temperature 25-26 ℃.
Described abamectin fermented waste water and pleurin fermentation waste water circulation process method, it is characterized in that and to produce the fermentation waste water that pleurin produces, the step 1) that is used for the fermentative production Avrmectin pumps into the pleurin fermented liquid in autoclave diaphragm half frame, at 6-12 kilogram/cm
2Pressure under carry out press filtration.
Described abamectin fermented waste water and pleurin fermentation waste water circulation process method, it is characterized in that and to produce the fermentation waste water that pleurin produces, be used for the interpolation of nutritive ingredient of the step 3) A Fuman streptomycete of fermentative production Avrmectin: the weight by filtrate adds starch 10-11%, soybean cake powder 1-1.5%, yeast powder 1-1.5%.
Described abamectin fermented waste water and pleurin fermentation waste water circulation process method, it is characterized in that and to produce the fermentation waste water that pleurin produces, after being used for the step 3) accent filtrate PH to 7.0-7.5 of fermentative production Avrmectin, weight by filtrate adds glucose 0.5-1.5%, ammonium sulfate 0.5-1.0%, potassium primary phosphate 0.015-0.02%, dipotassium hydrogen phosphate 0.0001-0.001%, the sal epsom 0.02-0.08% of 7 water, the U-Ramin MC 0.01-0.05% of sodium-chlor 0.01%-0.05% and 2 water, 117 ℃ of sterilizations are after 30 minutes, be cooled to 28 ℃, in above-mentioned filtrate, inoculate the yeast starter liquid of filtrate weight 10-40% again, after 28 ℃ of temperature are cultivated 48-72 hour down, regulate the PH to 7.0-7.5 of yeast culture liquid, add the nutritive ingredient of cultivating the A Fuman streptomycete then, sterilized 30 minutes for 121 ℃, after being cooled to 28 ℃, inoculation A Fuman streptomycete liquid seeds was cultivated 256-280 hour down for 28 ℃ in temperature.
Described abamectin fermented waste water and pleurin fermentation waste water circulation process method, it is characterized in that the interpolation of the nutritive ingredient of oblique top bacterium: the weight by yeast culture liquid adds corn steep liquor 5%, soybean cake powder 1%, and in real time stream adds glucose in culturing process, reaches 8-12% until the weight of glucose.
Described abamectin fermented waste water and pleurin fermentation waste water circulation process method is characterized in that the interpolation of the nutritive ingredient of A Fuman streptomycete: the weight by yeast culture liquid adds starch 10-14%, soybean cake powder 1-3%, yeast powder 1-2%.
Described abamectin fermented waste water and pleurin fermentation waste water circulation process method, after it is characterized in that transferring filtrate PH to 7.0-7.5, by weight adding glucose 1-1.5%, ammonium sulfate 0.5-0.8%, potassium primary phosphate 0.015-0.018%, dipotassium hydrogen phosphate 0.0005-0.001%, sal epsom 0.02-0.06%, the sodium-chlor 0.01%-0.03% of 7 water and the U-Ramin MC 0.01-0.03% of 2 water of filtrate.
Above-mentioned abamectin fermented waste water and pleurin fermentation waste water circulation process method, abamectin fermented waste water is used for the fermentative production pleurin, the pleurin fermentation waste water is used for the fermentative production Avrmectin, reach recycling of two kinds of waste water, from then on can stop the arbitrarily phenomenon of discharging of abamectin fermented waste water and pleurin fermentation waste water, reached the effect of environmental protection, also make simultaneously the utilization once again of two kinds of waste water, and utility value height, experiment shows tiring quite of product that tiring of the product that obtains that utilizes these two kinds of treated wastewater fermentations obtains with natural water by fermentation, method of the present invention has also reduced the production cost of enterprise, save the environmental protection investments and the processing costs of wastewater treatment aspect, utilize method processing waste water of the present invention and traditional method to compare, cost can reduce 60-70%.
Embodiment
Below further specify the present invention by specific embodiment.
Embodiment 1:
With producing the fermentation waste water that Avrmectin produces, be used for the fermentative production pleurin
1) the abamectin fermented liquid pump that will ferment 265 hours is gone in autoclave diaphragm half frame, at 6 kilograms/cm
2Pressure under carry out press filtration, obtain mycelia filter cake and filtrate, the filtrate of gained is abamectin fermented waste water, 2) PH to 10 of the abamectin fermented waste water of accent step 1) gained, be warming up to 70 ℃ then, be incubated after 20 minutes, cool off, filter again, 3) with step 2) filtrate of gained squeezes in the fermentor tank, and accent filtrate PH to 7.0, add the nutritive ingredient of cultivating the oblique top bacterium then, promptly the weight by filtrate adds corn steep liquor 5%, soybean cake powder 1%, sterilized 30 minutes for 117 ℃, be cooled to 25-26 ℃, press 10% inoculation oblique top bacteria liquid seed of nutrient solution weight, cultivated 280-300 hour down for 25 ℃ in temperature, and stream adds glucose in culturing process, keeps in the fermented liquid glucose content at 0.5-2%.
Experiment 10M
3Fermentor tank has carried out 10 batches experiment, 5 batches of control groups (fermenting) wherein with tap water, and 5 batches is experimental group (with the wastewater fermentation of abamectin fermented generation), experimental result such as following table.
| (ug/ml) tires | |
| Control group 1 | ????8926 |
| Control group 2 | ????9132 |
| Control group 3 | ????9838 |
| Control group 4 | ????9833 |
| Control group 5 | ????9435 |
| Mean level (ML) | ????9433 |
| Tire | |
| Experimental group 1 | ????9134 |
| Experimental group 2 | ????9235 |
| Experimental group 3 | ????8436 |
| Experimental group 4 | ????9942 |
| Experimental group 5 | ????9631 |
| Mean level (ML) | ????9276 |
The result shows: what the fermentation of tiring and utilizing nature water to carry out pleurin that the fermentation of adopting the abamectin fermented waste water of handling to carry out pleurin produces produced tires quite.
With producing the fermentation waste water that pleurin produces, be used for the fermentative production Avrmectin
1) the pleurin fermented liquid that will ferment 280 hours pumps in autoclave diaphragm half frame, at 6 kilograms/cm
2Pressure under carry out press filtration, obtain mycelia filter cake and filtrate, the filtrate of gained is the pleurin fermentation waste water, 2) PH to 10 of the pleurin fermentation waste water of accent step 1) gained, be warming up to 70 ℃ then, be incubated after 20 minutes, cool off, filter, 3) with step 2) filtrate of gained squeezes in the fermentor tank, and transfer filtrate PH to 7.2, and add the nutritive ingredient of cultivating the A Fuman streptomycete then, promptly the weight by filtrate adds starch 11%, soybean cake powder 1.5%, 1%, 121 ℃ of yeast powder sterilization 30 minutes, be cooled to 28 ℃ after, inoculation A Fuman streptomycete liquid seeds 10% was cultivated 256-280 hour down for 28 ℃ in temperature.
Experiment 10M
3Fermentor tank has carried out 10 batches experiment, 5 batches of control groups (fermenting) wherein with tap water, and 5 batches is experimental group (wastewater fermentation that produces with the pleurin fermentation), experimental result such as following table.
| (ug/vml) tires | |
| Control group 1 | ????3500 |
| Control group 2 | ????3308 |
| Control group 3 | ????3654 |
| Control group 4 | ????3386 |
| Control group 5 | ????3767 |
| Mean level (ML) | ????3523 |
| Experimental group 1 | ????3479 |
| Experimental group 2 | ????3548 |
| Experimental group 3 | ????3776 |
| Experimental group 4 | ????3467 |
| Experimental group 5 | ????3324 |
| Mean level (ML) | ????3519 |
The result shows: what the fermentation of tiring and utilizing nature water to carry out Avrmectin that the fermentation of adopting the pleurin fermentation waste water of handling to carry out Avrmectin produces produced tires quite.
In embodiment 1, will produce the fermentation waste water that Avrmectin produces, the processing step step 1) that is used for the fermentative production pleurin adopts 270,275 or 280 hours abamectin fermented liquid of fermentation to handle; Step 2) adopt to transfer the PH to 11 or 12 of abamectin fermented waste water in, be warming up to 75 or 80 ℃ then, be incubated 25 or 30 minutes, the tiring of the pleurin that obtains at last also can reach the effect identical with embodiment 1.
In embodiment 1, will produce the fermentation waste water that pleurin produces, the processing step step 1) that is used for the fermentative production Avrmectin adopts 285,290 or 300 hours abamectin fermented liquid of fermentation to handle; Step 2) adopts the PH to 11 or 12 that transfers abamectin fermented waste water in, be warming up to 75 or 80 ℃ then, be incubated 25 or 30 minutes; Transfer the PH to 7.2 or 7.5 of filtrate in the step 3), the nutritive ingredient that adds the oblique top bacterium be promptly by the weight adding starch 10% of filtrate, soybean cake powder 1%, and yeast powder 1.5%, the tiring of the Avrmectin that obtains at last also can reach the effect identical with embodiment 1.
Embodiment 2:
With producing the fermentation waste water that Avrmectin produces, be used for the fermentative production pleurin
1) the abamectin fermented liquid pump that will ferment 280 hours is gone in autoclave diaphragm half frame, at 10 kilograms/cm
2Pressure under carry out press filtration, obtain mycelia filter cake and filtrate, the filtrate of gained is abamectin fermented waste water, 2) PH to 11 of the abamectin fermented waste water of accent step 1) gained, be warming up to 80 ℃ then, be incubated after 25 minutes, cool off, filter again, 3) with step 2) filtrate of gained squeezes in the fermentor tank, and accent filtrate PH to 7.3, weight by filtrate adds glucose 1% then, ammonium sulfate 0.5%, potassium primary phosphate 0.015%, dipotassium hydrogen phosphate 0.0005%, magnesium sulfate heptahydrate 0.05%, sodium-chlor 0.01% and two water U-Ramin MCs 0.01%, 117 ℃ of sterilizations are after 30 minutes, be cooled to 28 ℃, in above-mentioned filtrate, inoculate the cereuisiae fermentum seed liquor of filtrate weight 20% again, after 28 ℃ of temperature are cultivated 48 hours down, get yeast culture liquid, regulate the PH to 7.2 of yeast culture liquid, add the nutritive ingredient of cultivating the oblique top bacterium then, promptly the weight by yeast culture liquid adds corn steep liquor 5%, 1%, 117 ℃ of soybean cake powder sterilization 30 minutes, be cooled to 26 ℃ after, press 10% inoculation oblique top bacteria liquid seed of nutrient solution weight, and in culturing process in real time stream add glucose, reach 10% until the weight of glucose, cultivated 280-300 hour down for 25 ℃ in temperature;
With producing the fermentation waste water that pleurin produces, be used for the fermentative production Avrmectin
1) the pleurin fermented liquid that will ferment 280 hours pumps in autoclave diaphragm half frame, at 6 kilograms/cm
2Pressure under carry out press filtration, obtain mycelia filter cake and filtrate, the filtrate of gained is the pleurin fermentation waste water, 2) PH to 10 of the pleurin fermentation waste water of accent step 1) gained, be warming up to 70 ℃ then, be incubated after 20 minutes, cool off, filter, 3) with step 2) filtrate of gained squeezes in the fermentor tank, and accent filtrate PH to 7.2, weight by filtrate adds glucose 0.5%, ammonium sulfate 1.0%, potassium primary phosphate 0.02%, dipotassium hydrogen phosphate 0.001%, the sal epsom 0.08% of 7 water, the U-Ramin MC 0.05% of sodium-chlor 0.05% and 2 water, 117 ℃ of sterilizations are after 30 minutes, be cooled to 28 ℃, in above-mentioned filtrate, inoculate the cereuisiae fermentum seed liquor of filtrate weight 40% again, after 28 ℃ of temperature are cultivated 72 hours down, regulate the PH to 7.2 of yeast culture liquid, add the nutritive ingredient of cultivating the A Fuman streptomycete then, promptly the weight by yeast culture liquid adds starch 12%, soybean cake powder 2.6%, 1%, 121 ℃ of yeast powder sterilization 30 minutes, be cooled to 28 ℃ after, inoculation A Fuman streptomycete liquid seeds 10% was cultivated 256-280 hour down for 28 ℃ in temperature.
Step 2 among the embodiment 2) adopts weight by filtrate to add the U-Ramin MC 0.03% of sal epsom 0.02%, sodium-chlor 0.03% and 2 water of glucose 1.5%, ammonium sulfate 0.8%, potassium primary phosphate 0.017%, dipotassium hydrogen phosphate 0.0001%, 7 water in, also can reach the technique effect of embodiment 2.
After measured, the waste water to ferment of handling through embodiment 2 also can reach the technique effect identical with embodiment 1.
Claims (10)
1. abamectin fermented waste water and pleurin fermentation waste water circulation process method is characterized in that being used for the fermentative production pleurin with producing the fermentation waste water that Avrmectin produces, and with producing the fermentation waste water that pleurin produces, are used for the fermentative production Avrmectin;
The described fermentation waste water that will produce the Avrmectin generation, be used for the fermentative production pleurin and comprise following processing step: the abamectin fermented liquid that 1) will ferment 265-280 hour carries out press filtration, obtain mycelia filter cake and filtrate, the filtrate of gained is abamectin fermented waste water, 2) PH to 10-12 of the abamectin fermented waste water of accent step 1) gained, be warming up to 70-80 ℃ then, be incubated after 20-30 minute, cool off, filter, 3) with step 2) filtrate of gained squeezes in the fermentor tank, and accent filtrate PH to 7.0-7.5, add to cultivate the nutritive ingredient of oblique top bacterium then, 117 ℃ of sterilizations 30 minutes, be cooled to 26 ℃ after, inoculation oblique top bacteria liquid seed was cultivated 280-300 hour down at temperature 25-26 ℃;
The described fermentation waste water that will produce the pleurin generation, be used for the fermentative production Avrmectin and comprise following processing step: the pleurin fermented liquid that 1) will ferment 280-300 hour carries out press filtration, obtain mycelia filter cake and filtrate, the filtrate of gained is the pleurin fermentation waste water, 2) PH to 10-12 of the pleurin fermentation waste water of accent step 1) gained, be warming up to 70-80 ℃ then, be incubated after 20-30 minute, cool off, filter, 3) with step 2) filtrate of gained squeezes in the fermentor tank, and accent filtrate PH to 7.0-7.5, add to cultivate the nutritive ingredient of A Fuman streptomycete then, 121 ℃ of sterilizations 30 minutes, be cooled to 28 ℃ after, inoculation A Fuman streptomycete liquid seeds was cultivated 256-280 hour down for 28 ℃ in temperature.
2. abamectin fermented waste water as claimed in claim 1 and pleurin fermentation waste water circulation process method, it is characterized in that and to produce the fermentation waste water that Avrmectin produces, the step 1) that is used for the fermentative production pleurin is gone into abamectin fermented liquid pump in autoclave diaphragm half frame, at 6-12 kilogram/cm
2Pressure under carry out press filtration.
3. abamectin fermented waste water as claimed in claim 1 and pleurin fermentation waste water circulation process method, it is characterized in that and to produce the fermentation waste water that Avrmectin produces, be used for the interpolation of nutritive ingredient of the step 3) oblique top bacterium of fermentative production pleurin: the weight by filtrate adds corn steep liquor 5%, soybean cake powder 1%, stream adds glucose in culturing process, and the level of keeping fermented liquid glucose is at 0.5-2%.
4. abamectin fermented waste water as claimed in claim 1 and pleurin fermentation waste water circulation process method, it is characterized in that and to produce the fermentation waste water that Avrmectin produces, after being used for the step 3) accent filtrate PH to 7.0-7.5 of fermentative production pleurin, weight by filtrate adds glucose 0.5-1.5%, ammonium sulfate 0.5-1.0%, potassium primary phosphate 0.015-0.02%, dipotassium hydrogen phosphate 0.0001-0.001%, the sal epsom 0.02-0.08% of 7 water, the U-Ramin MC 0.01-0.05% of sodium-chlor 0.01%-0.05% and 2 water, 117 ℃ of sterilizations are after 30 minutes, be cooled to 28 ℃, in above-mentioned filtrate, inoculate the yeast starter liquid of filtrate weight 10-40% again, after 28 ℃ of temperature are cultivated 48-72 hour down, get yeast culture liquid, regulate the PH to 7.0-7.5 of yeast culture liquid, add the nutritive ingredient of cultivating the oblique top bacterium then, sterilized 30 minutes for 117 ℃, after being cooled to 26 ℃, inoculation oblique top bacteria liquid seed was cultivated 280-300 hour down at temperature 25-26 ℃.
5. abamectin fermented waste water as claimed in claim 1 and pleurin fermentation waste water circulation process method, it is characterized in that and to produce the fermentation waste water that pleurin produces, the step 1) that is used for the fermentative production Avrmectin pumps into the pleurin fermented liquid in autoclave diaphragm half frame, at 6-12 kilogram/cm
2Pressure under carry out press filtration.
6. abamectin fermented waste water as claimed in claim 1 and pleurin fermentation waste water circulation process method, it is characterized in that and to produce the fermentation waste water that pleurin produces, be used for the interpolation of nutritive ingredient of the step 3) A Fuman streptomycete of fermentative production Avrmectin: the weight by filtrate adds starch 10-11%, soybean cake powder 1-1.5%, yeast powder 1-1.5%.
7. abamectin fermented waste water as claimed in claim 1 and pleurin fermentation waste water circulation process method, it is characterized in that and to produce the fermentation waste water that pleurin produces, after being used for the step 3) accent filtrate PH to 7.0-7.5 of fermentative production Avrmectin, weight by filtrate adds glucose 0.5-1.5%, ammonium sulfate 0.5-1.0%, potassium primary phosphate 0.015-0.02%, dipotassium hydrogen phosphate 0.0001-0.001%, the sal epsom 0.02-0.08% of 7 water, the U-Ramin MC 0.01-0.05% of sodium-chlor 0.01%-0.05% and 2 water, 117 ℃ of sterilizations are after 30 minutes, be cooled to 28 ℃, in above-mentioned filtrate, inoculate the yeast starter liquid of filtrate weight 10-40% again, after 28 ℃ of temperature are cultivated 48-72 hour down, regulate the PH to 7.0-7.5 of yeast culture liquid, add the nutritive ingredient of cultivating the A Fuman streptomycete then, sterilized 30 minutes for 121 ℃, after being cooled to 28 ℃, inoculation A Fuman streptomycete liquid seeds was cultivated 256-280 hour down for 28 ℃ in temperature.
8. abamectin fermented waste water as claimed in claim 4 and pleurin fermentation waste water circulation process method, it is characterized in that the interpolation of the nutritive ingredient of oblique top bacterium: the weight by yeast culture liquid adds corn steep liquor 5%, soybean cake powder 1%, and in real time stream adds glucose in culturing process, reaches 8-12% until the weight of glucose.
9. abamectin fermented waste water as claimed in claim 7 and pleurin fermentation waste water circulation process method, it is characterized in that the interpolation of the nutritive ingredient of A Fuman streptomycete: the weight by yeast culture liquid adds starch 10-14%, soybean cake powder 1-3%, yeast powder 1-2%.
10. as claim 4 or 10 described abamectin fermented waste waters and pleurin fermentation waste water circulation process method, after it is characterized in that transferring filtrate PH to 7.0-7.5, by weight adding glucose 1-1.5%, ammonium sulfate 0.5-0.8%, potassium primary phosphate 0.015-0.018%, dipotassium hydrogen phosphate 0.0005-0.001%, sal epsom 0.02-0.06%, the sodium-chlor 0.01%-0.03% of 7 water and the U-Ramin MC 0.01-0.03% of 2 water of filtrate.
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Cited By (3)
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| CN101665817B (en) * | 2009-09-28 | 2011-02-16 | 浙江升华拜克生物股份有限公司 | Recycling method of abamectin fermented waste water |
| CN104489241A (en) * | 2014-12-10 | 2015-04-08 | 内蒙古新威远生物化工有限公司 | Method for treating avermectin fermentation wastewater and producing biological feed |
| CN112094751A (en) * | 2020-07-22 | 2020-12-18 | 上海市农业科学院 | Fermentation product of stropharia rugoso-annulata QYL-10 and biological additive produced by fermentation product and application of fermentation product |
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| CN1163617C (en) * | 2000-10-30 | 2004-08-25 | 武汉绿世纪生物工程有限责任公司 | Process for preparing evericin by fermentation method |
| CN101319234B (en) * | 2008-07-04 | 2011-02-16 | 江苏赛奥生化有限公司 | Fermentation method for pleuromulin |
| CN101407775B (en) * | 2008-11-27 | 2010-09-08 | 中国科学院微生物研究所 | Method for preparing avermectin and special bacterial strain thereof |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101665817B (en) * | 2009-09-28 | 2011-02-16 | 浙江升华拜克生物股份有限公司 | Recycling method of abamectin fermented waste water |
| CN104489241A (en) * | 2014-12-10 | 2015-04-08 | 内蒙古新威远生物化工有限公司 | Method for treating avermectin fermentation wastewater and producing biological feed |
| CN104489241B (en) * | 2014-12-10 | 2018-11-02 | 内蒙古新威远生物化工有限公司 | A method of processing abamectin fermented waste water simultaneously produces biological feedstuff |
| CN112094751A (en) * | 2020-07-22 | 2020-12-18 | 上海市农业科学院 | Fermentation product of stropharia rugoso-annulata QYL-10 and biological additive produced by fermentation product and application of fermentation product |
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