Summary of the invention
At the problem of prior art existence, thereby the objective of the invention is to design the technical scheme that provides a kind of environmental protection, water reuse to be worth the fermentation waste water recycle method of high conserve water resource.
Described abamectin fermented waste water recycle method is characterized in that being used further to the fermentative production Avrmectin with producing the fermentation waste water that Avrmectin produces after acid, alkali and yeast are handled, and specifically comprises following processing step:
1) the abamectin fermented liquid that will ferment 255-280 hour carries out press filtration, obtains mycelia filter cake and filtrate, and the filtrate of gained is abamectin fermented waste water;
2) with the abamectin fermented waste water of step 1) gained respectively via hole diameter be that the strainer of 10 microns, 5 microns and 1 micron filters;
3) with sour menstruation regulating step 2) the PH to 0.5-2.5 of abamectin fermented waste water, be warming up to 80-90 ℃ again, be incubated after 30-40 minute, transfer PH to 10-13 with alkali, continue insulation after 30-40 minute, cool off, filter through sheet frame and more medium filter then;
4) will squeeze in the fermentor tank through the abamectin fermented waste water of step 3), transfer PH to 7.0-7.5, the weight of pressing waste water then adds glucose 0.5-1.5%, ammonium sulfate 0.05-0.25%, potassium primary phosphate 0.005-0.01%, dipotassium hydrogen phosphate 0.0001-0.001%, 117 ℃ of sterilizations are after 30 minutes, be cooled to 28 ℃, inoculation yeast seed liquor in above-mentioned waste water after cultivating 48-72 hour under temperature 27-30 ℃, gets yeast culture liquid again;
5) PH to 7.0-7.5 of adjusting yeast culture liquid adds the nutritive ingredient of cultivating the A Fuman streptomycete then, sterilizes 30-40 minute for 121 ℃, after being cooled to 28 ℃, inoculation A Fuman streptomycete liquid seeds after cultivating 250-280 hour under temperature 27-29 ℃, obtains abamectin fermented liquid;
6) the abamectin fermented liquid that step 5) is obtained is applied to abamectin fermented through the above-mentioned steps circular treatment again.
Described abamectin fermented waste water recycle method is characterized in that the acid described in the step 3) is phosphoric acid and vitriolic mixed solution, and described alkali is milk of lime or sodium hydroxide.
Described abamectin fermented waste water recycle method is characterized in that transferring PH to 1-2 with acid in the step 3), transfers PH to 11-12 with alkali.
Described abamectin fermented waste water recycle method is characterized in that adding in the step 4) glucose 1.0-1.5%, ammonium sulfate 0.05-0.20%, potassium primary phosphate 0.006-0.009%, dipotassium hydrogen phosphate 0.0002-0.001% in abamectin fermented waste water.
Described abamectin fermented waste water recycle method is characterized in that the yeast described in the step 4) is candiyeast or cereuisiae fermentum.
Above-mentioned abamectin fermented waste water recycle method, abamectin fermented waste water after handling, is handled with yeast bronsted lowry acids and bases bronsted lowry again, the containing saccharomycetic nutrient solution and can be used further to the fermentative production Avrmectin of gained, test shows that fermentation waste water can use repeatedly after acid, alkali and yeast comprehensive treating process, to not influence of fermentation titer.Use the utilization that present method can improve water resources significantly, reduce environmental pollution, with the method for wastewater treatment of routine relatively, production cost does not increase.
Embodiment
Below further specify the present invention by specific embodiment.
Embodiment 1: when producing Avrmectin with the abamectin fermented waste water of handling and not handling, fermentation titer relatively.
Experimental group a: cultivate the A Fuman streptomycete with tap water.
Experimental group b: use without the abamectin fermented waste water of any processing and cultivate the A Fuman streptomycete.
Experimental group c: be reused for the A Fuman streptomycete after abamectin fermented waste water handled with the following method again and cultivate.
Treatment process: the filter press that with waste water earlier is 10 microns with the filter cloth aperture; Its filtrate is filtered with the strainer of aperture less than 2.5 microns again; The smart filtrate that obtains is used phosphoric acid and vitriolic mixed solution (phosphoric acid: sulfuric acid=1: 2, volume ratio) transferring pH is 0.5, after being warmed up to 80 ℃, be incubated 30 minutes, transfer pH to 10 with milk of lime again, insulation is handled after about 40 minutes, cool to about 30 ℃, earlier through Plate Filtration (filter cloth aperture 5-10 micron), and after the filtration of the strainer of 1-2.5 micron, resulting filtrate transfers pH to filter through more medium filter (quartz sand-gac-quartz sand) after 7.0 with acid again, the waste water of handling through bronsted lowry acids and bases bronsted lowry that obtains, this waste water can directly be used in cultivates the A Fuman streptomycete, promptly adds the starch 10-11% of waste water weight, soybean cake powder 2-3%, yeast powder 0.5-1% and lime carbonate 0.1-0.5%, 121 ℃ the sterilization 30-40 minute, be cooled to 28 ℃ after, inoculation A Fuman streptomycete liquid seeds is measured after cultivating 250-280 hour under temperature 27-29 ℃ and is tired.
Experimental group d: with the waste water culturing yeast bacterium (used yeast is candiyeast or cereuisiae fermentum) of handling through bronsted lowry acids and bases bronsted lowry of experimental group c gained.Concrete grammar is that to add weight ratio in the waste water of handling through bronsted lowry acids and bases bronsted lowry be 1.5% glucose or molasses, 0.15% ammonium sulfate, 0.009% potassium primary phosphate, 0.001% dipotassium hydrogen phosphate, 117 ℃ of sterilizations are after 30 minutes then, cooling, press 10-50% amount inoculation yeast liquid spawn, cultivated 48 hours at 28-29 ℃, get yeast culture liquid, come fermentation culture A Fuman streptomycete with this yeast culture liquid, promptly regulate the PH to 7.0-7.5 of yeast culture liquid, add the starch 10-11% of yeast culture liquid weight meter then, soybean cake powder 2-3%, yeast powder 0.5-1% and lime carbonate 0.1-0.5%, 121 ℃ the sterilization 30-40 minute, be cooled to 28 ℃ after, inoculation A Fuman streptomycete liquid seeds is measured after cultivating 250-280 hour under temperature 27-29 ℃ and is tired.
Experimental group e: abamectin fermented waste water earlier with Plate Filtration (the filter cloth aperture is the 5-10 micron), is then being filtered with the strainer of aperture less than 2.5 microns; It is about 2.5 that filtrate is transferred pH with phosphoric acid and sulfuric acid (1: 2) mixed solution, then transfer pH11-13 with milk of lime again, be pressed in the storage pond, under greater than 25-32 ℃ of condition, placed 1-2 days, afterwards respectively through sheet frame (filter cloth aperture 5-10 micron), strainer (aperture is less than 2.5 microns) and more medium filter (medium is quartz sand and gac) filter, the filtrate (calling A liquid in the following text) of gained is transferred pH 6.8-7.2, be pressed in the fermentation vat then, after placing 2 days under the 25-32 ℃ of condition, promptly can be used for cultivating the A Foman streptomycete, promptly regulate the PH to 7.0-7.5 of yeast culture liquid, the starch 10-11% that adds yeast culture liquid weight meter then, soybean cake powder 2-3%, yeast powder 0.5-1% and lime carbonate 0.1-0.5%, 121 ℃ the sterilization 30-40 minute, be cooled to 28 ℃ after, inoculation A Fuman streptomycete liquid seeds is measured after cultivating 250-280 hour under temperature 27-29 ℃ and is tired.
Annotate: contain the yeast that comes out through seed selection in the fermentation vat, the meter bacterium of giving money as a gift is dense greater than 0.05%, and the bacterium liquid measure in the fermentation vat should be greater than more than 2 times of A liquid measure.
Above-mentioned a, b, c, d, five groups of experimental results of e see the following form:
More than every group of experiment at 5m
3Experimental result in the fermentor tank; Fermentation titer (ug/ml) be 5 the experiment batch mean value.
Embodiment 2:
1) the abamectin fermented liquid that will ferment 255-280 hour carries out press filtration, obtains mycelia filter cake and filtrate, and the filtrate of gained is abamectin fermented waste water;
2) with the abamectin fermented waste water of step 1) gained respectively via hole diameter be that the strainer of 10 microns, 5 microns and 1 micron filters;
3) with phosphoric acid and sulfuric acid mixture liquid menstruation regulating step 2) the PH to 2.5 of abamectin fermented waste water, be warming up to 90 ℃ again, be incubated after 35 minutes, transfer PH to 12 with milk of lime or sodium hydroxide, continue insulation after 35 minutes, cool off, filter through sheet frame and more medium filter then;
4) will squeeze in the fermentor tank through the abamectin fermented waste water of step 3), transfer PH to 7.5, the weight of pressing waste water then adds glucose 0.5%, ammonium sulfate 0.25%, potassium primary phosphate 0.01%, dipotassium hydrogen phosphate 0.001%, 117 ℃ of sterilizations are after 30 minutes, be cooled to 28 ℃, the yeast starter liquid of inoculation 20% inoculum size in above-mentioned waste water after cultivating 48-72 hour under temperature 27-30 ℃, gets yeast culture liquid again;
5) PH to 7.5 of adjusting yeast culture liquid, adding the nutritive ingredient of cultivating the A Fuman streptomycete then is starch 10-11%, soybean cake powder 2-3%, yeast powder 0.5-1% and lime carbonate 0.1-0.5%, 121 ℃ of sterilizations 30 minutes, be cooled to 28 ℃ after, inoculation A Fuman streptomycete liquid seeds, after cultivating 250-280 hour under temperature 27-29 ℃, obtain abamectin fermented liquid;
6) the abamectin fermented liquid that step 5) is obtained utilizes through the above-mentioned steps circular treatment.
The experiment proved that, repeatedly after the circulation, its Avrmectin reaches tire similar with tiring of circular treatment first.Experimental group d can obtain identical technique effect among embodiment 2 and the embodiment 1.
Among the embodiment 2: the PH to 1.5 or 2 of abamectin fermented waste water employing menstruation regulating step 2 in the step 3)), be warming up to 85 or 80 ℃ again, be incubated after 40 or 30 minutes, transfer PH to 10,11 or 13 with milk of lime, continue insulation 30 or minute its also can reach the technique effect identical with embodiment 2.
Adopt to transfer PH to 7.0 or 7.2 in the step 4), press weight adding glucose 1.5%, ammonium sulfate 0.05%, potassium primary phosphate 0.005%, the dipotassium hydrogen phosphate 0.0001% of waste water then, it also can reach the technique effect identical with embodiment 2.