CN107927321B - Natural concentrated dietary fiber and preparation method thereof - Google Patents
Natural concentrated dietary fiber and preparation method thereof Download PDFInfo
- Publication number
- CN107927321B CN107927321B CN201711043437.3A CN201711043437A CN107927321B CN 107927321 B CN107927321 B CN 107927321B CN 201711043437 A CN201711043437 A CN 201711043437A CN 107927321 B CN107927321 B CN 107927321B
- Authority
- CN
- China
- Prior art keywords
- dietary fiber
- enzymolysis
- pine bark
- water
- natural concentrated
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 235000013325 dietary fiber Nutrition 0.000 title claims abstract description 72
- 238000002360 preparation method Methods 0.000 title claims abstract description 18
- 235000010204 pine bark Nutrition 0.000 claims abstract description 44
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 44
- 239000000463 material Substances 0.000 claims abstract description 36
- 240000006024 Lactobacillus plantarum Species 0.000 claims abstract description 31
- 235000013965 Lactobacillus plantarum Nutrition 0.000 claims abstract description 31
- 229940072205 lactobacillus plantarum Drugs 0.000 claims abstract description 31
- 238000000855 fermentation Methods 0.000 claims abstract description 26
- 230000004151 fermentation Effects 0.000 claims abstract description 26
- 239000002994 raw material Substances 0.000 claims abstract description 25
- 238000002791 soaking Methods 0.000 claims abstract description 18
- 238000010438 heat treatment Methods 0.000 claims abstract description 14
- 239000007788 liquid Substances 0.000 claims abstract description 12
- 239000002351 wastewater Substances 0.000 claims abstract description 10
- 238000001035 drying Methods 0.000 claims abstract description 8
- 238000001816 cooling Methods 0.000 claims abstract description 7
- 239000011259 mixed solution Substances 0.000 claims abstract description 7
- 238000007873 sieving Methods 0.000 claims abstract description 7
- 229940088598 enzyme Drugs 0.000 claims description 29
- 108090000790 Enzymes Proteins 0.000 claims description 28
- 102000004190 Enzymes Human genes 0.000 claims description 28
- 230000000694 effects Effects 0.000 claims description 27
- 108090000526 Papain Proteins 0.000 claims description 16
- 239000004365 Protease Substances 0.000 claims description 16
- 229940055729 papain Drugs 0.000 claims description 16
- 235000019834 papain Nutrition 0.000 claims description 16
- 108010059820 Polygalacturonase Proteins 0.000 claims description 15
- 108010093305 exopolygalacturonase Proteins 0.000 claims description 15
- 238000000034 method Methods 0.000 claims description 11
- 235000011611 Pinus yunnanensis Nutrition 0.000 claims description 9
- 241000018652 Pinus yunnanensis Species 0.000 claims description 9
- 239000006041 probiotic Substances 0.000 claims description 7
- 235000018291 probiotics Nutrition 0.000 claims description 7
- 239000012535 impurity Substances 0.000 claims description 6
- 239000000758 substrate Substances 0.000 claims description 6
- 238000004140 cleaning Methods 0.000 claims description 5
- 241000218652 Larix Species 0.000 claims description 4
- 235000005590 Larix decidua Nutrition 0.000 claims description 4
- 235000011609 Pinus massoniana Nutrition 0.000 claims description 4
- 241000018650 Pinus massoniana Species 0.000 claims description 4
- 239000001913 cellulose Substances 0.000 claims description 4
- 229920002678 cellulose Polymers 0.000 claims description 4
- 235000005911 diet Nutrition 0.000 claims description 4
- 230000000378 dietary effect Effects 0.000 claims description 4
- 150000001875 compounds Chemical class 0.000 claims description 2
- 238000002156 mixing Methods 0.000 claims description 2
- 238000009210 therapy by ultrasound Methods 0.000 claims description 2
- 239000000835 fiber Substances 0.000 abstract description 21
- 238000004519 manufacturing process Methods 0.000 abstract description 7
- 230000002522 swelling effect Effects 0.000 abstract description 5
- OBMBUODDCOAJQP-UHFFFAOYSA-N 2-chloro-4-phenylquinoline Chemical compound C=12C=CC=CC2=NC(Cl)=CC=1C1=CC=CC=C1 OBMBUODDCOAJQP-UHFFFAOYSA-N 0.000 abstract description 2
- 239000003599 detergent Substances 0.000 abstract description 2
- 238000002474 experimental method Methods 0.000 abstract description 2
- 230000007935 neutral effect Effects 0.000 abstract description 2
- 230000008961 swelling Effects 0.000 abstract description 2
- 239000000047 product Substances 0.000 description 19
- 108090000623 proteins and genes Proteins 0.000 description 9
- 241001465754 Metazoa Species 0.000 description 7
- 108020004465 16S ribosomal RNA Proteins 0.000 description 6
- 238000006243 chemical reaction Methods 0.000 description 6
- 230000012010 growth Effects 0.000 description 6
- FERIUCNNQQJTOY-UHFFFAOYSA-N Butyric acid Chemical compound CCCC(O)=O FERIUCNNQQJTOY-UHFFFAOYSA-N 0.000 description 4
- 241000186660 Lactobacillus Species 0.000 description 4
- 239000002253 acid Substances 0.000 description 4
- 230000009286 beneficial effect Effects 0.000 description 4
- 210000002421 cell wall Anatomy 0.000 description 4
- 210000001072 colon Anatomy 0.000 description 4
- 229940039696 lactobacillus Drugs 0.000 description 4
- 235000018102 proteins Nutrition 0.000 description 4
- 102000004169 proteins and genes Human genes 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 3
- 239000008272 agar Substances 0.000 description 3
- 239000003513 alkali Substances 0.000 description 3
- 244000052616 bacterial pathogen Species 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 210000004027 cell Anatomy 0.000 description 3
- 210000001035 gastrointestinal tract Anatomy 0.000 description 3
- 230000036541 health Effects 0.000 description 3
- 210000004877 mucosa Anatomy 0.000 description 3
- 235000016709 nutrition Nutrition 0.000 description 3
- 230000035764 nutrition Effects 0.000 description 3
- 230000001766 physiological effect Effects 0.000 description 3
- 229920001282 polysaccharide Polymers 0.000 description 3
- 239000005017 polysaccharide Substances 0.000 description 3
- 238000009631 Broth culture Methods 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- 241000192125 Firmicutes Species 0.000 description 2
- 244000199885 Lactobacillus bulgaricus Species 0.000 description 2
- 235000013960 Lactobacillus bulgaricus Nutrition 0.000 description 2
- 241000194020 Streptococcus thermophilus Species 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 235000014633 carbohydrates Nutrition 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 238000003745 diagnosis Methods 0.000 description 2
- XBDQKXXYIPTUBI-UHFFFAOYSA-N dimethylselenoniopropionate Natural products CCC(O)=O XBDQKXXYIPTUBI-UHFFFAOYSA-N 0.000 description 2
- 238000003912 environmental pollution Methods 0.000 description 2
- 210000003608 fece Anatomy 0.000 description 2
- 150000004676 glycans Chemical class 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 230000000968 intestinal effect Effects 0.000 description 2
- 210000004347 intestinal mucosa Anatomy 0.000 description 2
- 229940004208 lactobacillus bulgaricus Drugs 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 230000035790 physiological processes and functions Effects 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 238000004321 preservation Methods 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 230000001737 promoting effect Effects 0.000 description 2
- 238000005096 rolling process Methods 0.000 description 2
- 238000012163 sequencing technique Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 1
- 206010000117 Abnormal behaviour Diseases 0.000 description 1
- 235000017060 Arachis glabrata Nutrition 0.000 description 1
- 244000105624 Arachis hypogaea Species 0.000 description 1
- 235000010777 Arachis hypogaea Nutrition 0.000 description 1
- 235000018262 Arachis monticola Nutrition 0.000 description 1
- 235000016068 Berberis vulgaris Nutrition 0.000 description 1
- 241000335053 Beta vulgaris Species 0.000 description 1
- 241000252983 Caecum Species 0.000 description 1
- 102000016938 Catalase Human genes 0.000 description 1
- 108010053835 Catalase Proteins 0.000 description 1
- 206010010774 Constipation Diseases 0.000 description 1
- 206010012735 Diarrhoea Diseases 0.000 description 1
- IAJILQKETJEXLJ-UHFFFAOYSA-N Galacturonsaeure Natural products O=CC(O)C(O)C(O)C(O)C(O)=O IAJILQKETJEXLJ-UHFFFAOYSA-N 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 238000003794 Gram staining Methods 0.000 description 1
- 239000007995 HEPES buffer Substances 0.000 description 1
- 208000022559 Inflammatory bowel disease Diseases 0.000 description 1
- 208000005016 Intestinal Neoplasms Diseases 0.000 description 1
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 229920002230 Pectic acid Polymers 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- IAJILQKETJEXLJ-RSJOWCBRSA-N aldehydo-D-galacturonic acid Chemical compound O=C[C@H](O)[C@@H](O)[C@@H](O)[C@H](O)C(O)=O IAJILQKETJEXLJ-RSJOWCBRSA-N 0.000 description 1
- AEMOLEFTQBMNLQ-BKBMJHBISA-N alpha-D-galacturonic acid Chemical compound O[C@H]1O[C@H](C(O)=O)[C@H](O)[C@H](O)[C@H]1O AEMOLEFTQBMNLQ-BKBMJHBISA-N 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 239000005018 casein Substances 0.000 description 1
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 description 1
- 235000021240 caseins Nutrition 0.000 description 1
- 210000004534 cecum Anatomy 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 210000001198 duodenum Anatomy 0.000 description 1
- 239000000428 dust Substances 0.000 description 1
- 230000001804 emulsifying effect Effects 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 210000002919 epithelial cell Anatomy 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 210000003495 flagella Anatomy 0.000 description 1
- 230000002496 gastric effect Effects 0.000 description 1
- 230000005176 gastrointestinal motility Effects 0.000 description 1
- 235000003642 hunger Nutrition 0.000 description 1
- 239000010903 husk Substances 0.000 description 1
- 230000003301 hydrolyzing effect Effects 0.000 description 1
- 210000003405 ileum Anatomy 0.000 description 1
- 230000000415 inactivating effect Effects 0.000 description 1
- 201000002313 intestinal cancer Diseases 0.000 description 1
- 208000028774 intestinal disease Diseases 0.000 description 1
- 230000006799 invasive growth in response to glucose limitation Effects 0.000 description 1
- 238000005342 ion exchange Methods 0.000 description 1
- 208000002551 irritable bowel syndrome Diseases 0.000 description 1
- 210000001630 jejunum Anatomy 0.000 description 1
- 229920005610 lignin Polymers 0.000 description 1
- 210000003750 lower gastrointestinal tract Anatomy 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 235000019629 palatability Nutrition 0.000 description 1
- 235000020232 peanut Nutrition 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 239000010318 polygalacturonic acid Substances 0.000 description 1
- -1 polysaccharide carbohydrate Chemical class 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000000529 probiotic effect Effects 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 235000019260 propionic acid Nutrition 0.000 description 1
- 238000010298 pulverizing process Methods 0.000 description 1
- IUVKMZGDUIUOCP-BTNSXGMBSA-N quinbolone Chemical compound O([C@H]1CC[C@H]2[C@H]3[C@@H]([C@]4(C=CC(=O)C=C4CC3)C)CC[C@@]21C)C1=CCCC1 IUVKMZGDUIUOCP-BTNSXGMBSA-N 0.000 description 1
- 239000002516 radical scavenger Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 230000036186 satiety Effects 0.000 description 1
- 235000019627 satiety Nutrition 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 235000021391 short chain fatty acids Nutrition 0.000 description 1
- 150000004666 short chain fatty acids Chemical class 0.000 description 1
- 210000000813 small intestine Anatomy 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 238000011895 specific detection Methods 0.000 description 1
- 230000000087 stabilizing effect Effects 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 230000001551 toxigenic effect Effects 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 1
- 210000002438 upper gastrointestinal tract Anatomy 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 235000015099 wheat brans Nutrition 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/14—Pretreatment of feeding-stuffs with enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/111—Aromatic compounds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/169—Plantarum
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Food Science & Technology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Physiology (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Botany (AREA)
- Mycology (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
A natural concentrated dietary fiber and a preparation method thereof are disclosed, wherein the natural concentrated dietary fiber is prepared by crushing pine bark rich in dietary fiber, sieving, soaking in water, draining waste water after soaking, transferring the soaked pine bark to a reactor, adding water to obtain a pine bark mixed solution, carrying out ultrasonic-assisted enzymolysis treatment, draining the liquid after enzymolysis, inoculating lactobacillus plantarum HKB-38 into the material after enzymolysis for fermentation, carrying out high-temperature high-pressure heat treatment on the fermented material, cooling and drying. The invention also includes a preparation method of the natural concentrated dietary fiber. According to the invention, pine bark with low price and rich resources is selected as a production raw material, so that the production cost is greatly reduced, the resources are fully utilized, and a high additional value is created. The invention obviously improves the purity of the dietary fiber and the swelling property and water holding capacity of the fiber product. Experiments prove that the content of crude fiber in the concentrated dietary fiber is more than or equal to 60 percent, the content of Neutral Detergent Fiber (NDF) is more than or equal to 86 percent, the content of Acid Detergent Fiber (ADF) is more than or equal to 70 percent, the swelling capacity of the product is more than or equal to 5.0mL/g, and the water holding capacity is more than or equal to 5.0 g/g.
Description
Technical Field
The invention relates to dietary fiber and a preparation method thereof, in particular to natural concentrated dietary fiber and a preparation method thereof.
Background
The dietary fiber is a general name of polysaccharide carbohydrate and lignin which are not digested and absorbed by human bodies, and the physiological functions of the dietary fiber mainly comprise the following aspects: 1) promoting gastrointestinal motility and reducing constipation; 2) the scavenger has the function of reducing the transfer of pathogenic bacteria from the hindgut to the foregut, and certain component structures of the dietary fiber also have the function of adsorbing toxin and pathogenic bacteria; 3) the dietary fiber can stabilize blood sugar concentration and reduce blood cholesterol level; 4) the soluble/easily fermented dietary fiber is fermented in the colon, provides nutrition for microorganisms in the colon and is beneficial to stabilizing the balance of intestinal flora; 5) short chain fatty acids (acetic acid, propionic acid and butyric acid) generated by fiber fermentation inhibit the growth of pathogenic bacteria in turn, and in addition, the butyric acid can provide energy for colon mucosal cells, promote the proliferation of epithelial cells and maintain the integrity of intestinal mucosa, thereby being beneficial to the absorption of nutrients and preventing diarrhea; 6) the dietary fiber has water-holding swelling property, and can enhance satiety and reduce abnormal behaviors caused by animal hunger.
The use of dietary fiber has received increasing attention in the field of animal and human nutrition during the last 20 years. Although dietary fiber cannot directly provide nutrition, the dietary fiber has physiological characteristics of promoting health, and is currently considered as the seventh nutrient by medical and nutriologists at home and abroad. Especially, the physiological properties of the dietary fiber, such as fermentability, expansibility, water-holding capacity, adsorbability, ion exchange effect and the like, provide a physiological basis for the positive effect of the dietary fiber in animals. More and more researches show that a proper amount of dietary fiber can not only damage the health of animals, but also improve the growth condition and the production performance of the animals, and can form a reasonable daily ration structure and maintain the normal digestion function of the animal body while providing animal energy. The dietary fiber also has certain prevention and treatment effects on common intestinal diseases such as irritable bowel syndrome, inflammatory bowel disease and intestinal cancer, is beneficial to keeping normal physiological functions of the intestinal tract and repairing and improving damaged intestinal tract environment, and has important significance on maintaining intestinal tract health.
At present, the commonly used fiber sources in the feed are conventional feed raw materials such as wheat bran, soybean hull, beet pulp, corn bran and the like, the fiber content of the crude fiber raw materials is low (for example, the crude fiber content of the bran is generally not more than 10 percent), and the poor feed palatability is easy to cause when the addition amount is high; moreover, these materials have a high content of insoluble fibers and are difficult to ferment, resulting in low utilization. The preparation of dietary fibers with high cellulose content and good physicochemical properties is gaining more and more attention, for example CN105124134A discloses a method for preparing dietary fibers, which comprises the following steps: 1) pulverizing raw materials containing dietary cellulose, sieving, and soaking in water to obtain substrate solution; 2) emulsifying the substrate liquid to obtain an emulsified substrate liquid; 3) adding enzyme into the emulsified substrate solution for enzymolysis, inactivating the enzyme in the obtained enzymolysis solution, centrifuging, and collecting precipitate to obtain a filtrate; 4) inoculating probiotics into the filter medium, fermenting, sterilizing and drying to obtain the dietary fiber.
As a resource which is ignored by people for a long time and can create high added value, the pine bark is rich in components such as procyanidine and the like, and can also be used as a high-quality raw material for extracting/preparing the concentrated dietary fiber, but compared with the raw materials such as peanut shells, rice husks, corncobs and the like disclosed in CN105124134A, the pine bark is harder and has higher lignification degree. Therefore, in order to prepare dietary fiber from pine bark, the efficacy of pine bark must be improved with respect to the dietary fiber preparation method and process parameters.
Disclosure of Invention
The invention aims to realize the following technical scheme that a Lactobacillus plantarum HKB-38 is preserved in China center for type culture Collection (CCTCC for short) in 2016, 12 and 8 days, the preservation number is CCTCC NO: M2016727, the preservation unit address is as follows: wuhan, Wuhan university, zip code 430072.
Furthermore, the 16S rRNA gene sequence of the lactobacillus plantarum HKB-38 is shown as SEQ ID NO. 1.
The further purpose of the invention is realized by the following technical scheme that pine bark (preferably Yunnan pine bark, larch bark or masson pine bark, more preferably Yunnan pine bark) rich in dietary fiber is crushed and sieved, water (preferably normal-temperature clear water) is added for soaking, waste water is discharged after soaking, then the soaked pine bark is transferred into a reactor, water (preferably normal-temperature clear water) is added again to obtain pine bark mixed liquid, ultrasonic-assisted enzymolysis treatment is carried out, liquid is discharged after enzymolysis, the material after enzymolysis is inoculated into lactobacillus plantarum HKB-38 for fermentation, and finally the fermented material is subjected to high-temperature high-pressure heat treatment, cooling and drying to obtain the natural dietary concentrated fiber.
The further purpose of the invention is realized by the following technical scheme, and the method for naturally concentrating the dietary fiber comprises the following steps:
1) cleaning pine bark raw materials containing dietary cellulose, removing impurities, crushing, sieving with a 20-60-mesh sieve, and mixing the sieved pine bark raw materials with water according to a ratio of 1 g: 5-20 mL of the waste water is added with water for soaking for 2-10 hours, and waste water is discharged after soaking; the water soaking in the step can remove some water-soluble impurities, such as soil, dust, water-soluble protein, carbohydrate and the like, is beneficial to subsequent enzymolysis, and improves the purity of dietary fiber products;
2) transferring the soaked pine bark obtained in the step 1) into a reactor, wherein the weight ratio of the soaked pine bark to normal-temperature clear water is 1 g: 10-30 mL, adding water again to obtain a pine bark mixed solution, performing ultrasonic-assisted enzymolysis treatment, and draining the liquid after enzymolysis to obtain an enzymolysis material;
3) inoculating lactobacillus plantarum HKB-38 into the material subjected to enzymolysis in the step 2) for fermentation to obtain a fermented material;
4) and (3) performing heat treatment on the fermented material obtained in the step 3) for 100-60 min at the temperature of 350-400 ℃ and the pressure of 5-30 MPa, cooling and drying to obtain the product.
Further, in the step 2), the ultrasonic-assisted enzymolysis treatment is to perform ultrasonic treatment for 2-10 min, and then add an enzyme preparation for enzymolysis, wherein the enzymolysis temperature is 35-55 ℃, the enzymolysis pH value is 6.0-8.0, and the enzymolysis time is 20-60 min.
Further, the enzyme preparation is pectinase or/and papain; the enzyme activity of the pectinase is 0.5-5.0U/mg (preferably 1.0U/mg), and the ratio of the pectinase to the pine bark mixed solution is 1-2 g: 1L; the enzyme activity of the papain is 5-50U/mg (preferably 12U/mg), and the ratio of the papain to pine bark mixed liquor is 5-10 g: 1L of the compound. The enzyme activity of pectinase is defined as follows: under the conditions of 50 ℃ and pH value of 4.0, the enzyme amount required for decomposing polygalacturonic acid to generate 1 mu mol of galacturonic acid in 1min is 1 enzyme activity unit; the enzyme activity of papain is defined as follows: under the conditions of 40 ℃ and pH value of 7.2, the enzyme amount required for hydrolyzing casein to generate 1 mu g of tyrosine in 1min is 1 enzyme activity unit.
Further, in the step 3), the mass ratio of the lactobacillus plantarum HKB-38 to the material subjected to enzymolysis is 1-3 g: 1kg, the fermentation temperature is 35-42 ℃, and the fermentation time is 25-50 min.
Compared with the prior art, the invention has the advantages that:
1) the pine bark selected by the invention has rich sources of fiber raw materials and low price, thereby not only reducing the production cost, but also reducing the problems of resource waste, environmental pollution and the like caused by abandoning and burning the raw materials, and realizing the purpose of maximizing the resource utilization;
2) the invention is processed by ultrasonic-assisted enzyme method, enzymolysis is carried out to remove the pine bark cell content and protein and soluble polysaccharide substances in the cell wall (wherein pectase hydrolyzes pectic substance of the pine bark cell wall, papain hydrolyzes protein and proteoglycan and the like in the cell wall), the cell content and the protein and soluble polysaccharide substances in the cell wall are released to enter into aqueous solution and discharged through drainage, thereby realizing the concentration of dietary fiber, and simultaneously, the ultrasonic-assisted enzyme method is used for improving the enzymolysis efficiency, achieving the purposes of low energy consumption and low enzyme consumption, thereby reducing the cost;
3) the lactobacillus plantarum HKB-38 selected by the invention is gram-positive bacteria and has the characteristics of acid resistance, vigorous growth, strong acid production capacity, strong fermentation capacity and the like; the lactobacillus plantarum HKB-38 is used for fermentation, so that the fermentation effect on pine bark raw materials is better, the fermentation time is shortened, the natural and effective functions of the concentrated dietary fiber product are effectively guaranteed, and the physiological properties of the dietary fiber such as fermentability, water holding capacity, expansibility and the like are further enhanced;
4) the fibril is opened by high-temperature and high-pressure heat treatment, and the lignocellulose can multiply increase the water adsorption capacity in the high-temperature and high-pressure process, so that the water holding capacity of the dietary fiber product is greatly improved, and the effect of killing the added lactobacillus plantarum HKB-38 is also achieved;
5) compared with the conventional method for preparing dietary fiber by using an alkali method commonly used in commercialization at present, the method effectively avoids the loss of the dietary fiber and the damage to the physiological characteristics of the dietary fiber caused by alkali liquor treatment, and further reduces the problem of serious environmental pollution caused by the discharge of a large amount of alkali waste liquid.
Experiments prove that the natural concentrated dietary fiber product prepared by the invention has the crude fiber content of more than or equal to 60 percent, the Neutral Detergent Fiber (NDF) content of more than or equal to 86 percent, the Acid Detergent Fiber (ADF) content of more than or equal to 70 percent, the product swelling capacity of more than or equal to 5.0mL/g and the water holding capacity of more than or equal to 5.0 g/g.
Drawings
Various other advantages and benefits will become apparent to those of ordinary skill in the art upon reading the following detailed description of the preferred embodiments. The drawings are only for purposes of illustrating the preferred embodiments and are not to be construed as limiting the invention. Also, like reference numerals are used to refer to like parts throughout the drawings. In the drawings:
FIG. 1 is a microscope (1000X) view of Lactobacillus plantarum HKB-38 according to the invention.
FIG. 2 is a plate colony morphology of Lactobacillus plantarum HKB-38 of the invention.
FIG. 3 shows the phylogenetic tree of Lactobacillus plantarum HKB-38 according to the invention.
Detailed Description
Exemplary embodiments of the present disclosure will be described in more detail below with reference to the accompanying drawings. While exemplary embodiments of the present disclosure are shown in the drawings, it should be understood that the present disclosure may be embodied in various forms and should not be limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the scope of the disclosure to those skilled in the art.
Example 1: separation and identification of lactobacillus plantarum
1. Taking intestinal mucosa of healthy piglet small intestine (such as duodenum, jejunum, ileum, caecum, and colon) 5cm2Placing in a sterilized beaker, washing the content on the mucosa with sterile physiological saline, immersing the mucosa in a beaker containing 15mL of HEPES buffer solution, shaking the mucosa back and forth for 5min with forceps, sucking 0.2mL of supernatant with a sterile syringe, placing in a Hungates rolling tube containing Rogosa SL agar, rolling gently by hand to uniformly distribute the supernatant on the surface of the agar in the tube, and placing in 5% CO2Culturing in an incubator at 37 ℃ for 72h, performing aseptic operation in a biological safety cabinet, picking bacterial colonies with the size of a white needle point by using a needle head to culture in anaerobic sterile Rogosa broth, observing whether the broth becomes turbid, and selecting the culture broth with good growth and turbidity to be stored in a refrigerator at 4 ℃ for later use.
2. Gram-positive bacteria are identified by gram staining, and lactobacillus is preliminarily identified by a catalase test.
3. Acid-resistant breeding: respectively adopting a gastric lactobacillus broth culture medium and an intestinal lactobacillus broth culture medium for culture, observing the growth conditions in the period, and selecting the bacteria with the highest survival rate and the most vigorous growth to obtain a strain, namely HKB-38.
4. Finally, the morphology, the physiological and biochemical characteristics and the 16S rRNA gene sequence of the strain HKB-38 are identified by the China center for type culture Collection, and the specific detection results are as follows:
strain HKB-38, microscopic observation: is in the shape of slender rod with blunt ends, is single-grown, has no spore and flagellum, but can move, as shown in figure 1; plate observation: the bacterial colony grows on MRS agar medium, and is milky white, regular and circular, and has a convex center and a smooth and moist surface, as shown in figure 2.
The results of the physiological and biochemical characteristics test of the strain HKB-38 are shown in tables 1 and 2.
TABLE 1 physiological and biochemical characteristics of the strain HKB-38-enzyme activity, carbon source oxidation
+: positive reaction; -: negative reaction; w: weak positive reaction
TABLE 2 physiological and biochemical characteristics of strain HKB-38-production of acid Using a carbon Source
+: positive reaction; -: negative reaction; w-weak positive reaction
After sequencing, the gene sequence of HKB-3816S rRNA of the strain is shown as SEQ ID NO:1, and the gene sequencing BLAST result of the HKB-3816S rRNA of the strain shows that the strain has the highest homology with the 16S rRNA sequence of Lactobacillus subsp. The phylogenetic tree was plotted using correlation software, see FIG. 3. From a phylogenetic tree constructed by 16S rRNA of the strain, the strain HKB-38 and Lactobacillus plantarum (Lactobacillus plantarum) have a closest development relationship and are classified in the same group, and the sequence homology is 100%. Therefore, based on phylogenetic trees and 16S rRNA homology, the strain HKB-38 was determined to be a new member of the genus Lactobacillus plantarum. In conclusion, the results of colony characteristics, physiological and biochemical characteristics and 16S rRNA gene sequences are identified as Lactobacillus plantarum (Lactobacillus plantarum), and the Lactobacillus plantarum HKB-38 is classified and named.
Example 2: effect of different pine bark materials on Natural concentrated dietary fiber
1) Cleaning Yunnan pine bark, larch bark and masson pine bark respectively, removing impurities, crushing, sieving with a 20-mesh sieve, adding clear water at normal temperature for soaking, wherein the proportion of the crushed raw materials to the clear water at normal temperature is 1 g: 10mL, the soaking time is 4h, and the wastewater is drained.
2) Respectively transferring the three pine bark raw materials obtained in the step 1) to a reactor, and adding clear water again, wherein the ratio of the pine bark raw materials to the clear water at normal temperature is 1 g: and 20mL, carrying out ultrasonic-assisted enzymolysis, specifically, after ultrasonic pretreatment is carried out for 5min, adding 1g of pectinase (the pectinase with the enzyme activity of 1.0U/mg, which is sold by Sigma in the United states, and the number of the pectinase is 17389) and 5g of papain (the papain with the enzyme activity of 12U/mg, which is sold by Sigma in the United states, and the number of the papain is 76218) into each liter of mixed solution for enzymolysis, wherein the enzymolysis temperature is 42 ℃, the enzymolysis pH value is 6.5, the enzymolysis time is 30min, and draining the liquid after enzymolysis to obtain the material after enzymolysis.
3) Respectively inoculating lactobacillus plantarum HKB-38 into the three materials obtained in the step 2) after enzymolysis for fermentation, wherein the mass ratio of the lactobacillus plantarum HKB-38 to the materials is 1 g: 1kg, the fermentation temperature is 37 ℃, and the fermentation time is 25min, so as to obtain the fermented material.
4) Performing high-temperature high-pressure heat treatment on the three fermented materials obtained in the step 3) respectively at 350 ℃, 10MPa and 20min, cooling and drying to obtain three natural concentrated dietary fibers, and detecting each index, wherein the results are shown in Table 3.
TABLE 3 Effect of different pine bark materials on the Natural concentrated dietary fiber product
As can be seen from Table 3, under the same preparation method conditions, the natural concentrated dietary fiber products prepared from Yunnan pine bark, larch bark and masson pine bark respectively have the crude fiber content of more than 60 percent, the NDF content of more than 86 percent, the ADF content of more than 70 percent, the swelling property of the product of more than 5.0mL/g and the water holding capacity of more than 5.0 g/g; and the natural concentrated dietary fiber prepared from Yunnan pine bark has the best index parameter and can be used as the preferred raw material.
Example 3: effect of fermentation of different probiotics on Natural concentrated dietary fiber
1) Cleaning Yunnan pine bark, removing impurities, crushing, sieving with a 30-mesh sieve, adding normal-temperature clear water for soaking, wherein the proportion of the crushed raw materials to the normal-temperature clear water is 1 g: 5mL, soaking time is 2h, and wastewater is discharged.
2) Transferring the pine bark raw material obtained in the step 1) into a reactor, and adding clear water again, wherein the ratio of the pine bark raw material to the clear water at normal temperature is 1 g: 10mL, carrying out ultrasonic-assisted enzymolysis, specifically, after ultrasonic pretreatment for 10min, adding 1.2g of pectinase (the pectinase sold by Sigma in the United states and the serial number of 17389) with the enzyme activity of 1.0U/mg and 6g of papain (the papain sold by Sigma in the United states and the serial number of 76218) with the enzyme activity of 12U/mg into each liter of mixed solution for enzymolysis, wherein the enzymolysis temperature is 55 ℃, the enzymolysis pH value is 6.0, the enzymolysis time is 20min, and draining the liquid after enzymolysis to obtain the material after enzymolysis.
3) Respectively inoculating lactobacillus plantarum HKB-38, lactobacillus bulgaricus powder (purchased from Nanjing feces diagnosis biotechnology limited, product number is BZW23003, and bacterium number is CICC6032) or streptococcus thermophilus powder (purchased from Nanjing feces diagnosis biotechnology limited, product number is BZW23104, and bacterium number is AS1.1864) into the material obtained in the step 2) after enzymolysis for fermentation, wherein the mass ratio of probiotics to the material is 2 g: 1kg, fermenting at 40 deg.C for 30min to obtain fermented material.
4) Respectively carrying out high-temperature high-pressure heat treatment on the fermented materials obtained in the step 3), cooling and drying at the temperature of 400 ℃, the pressure of 5MPa and the treatment time of 30min to obtain three natural concentrated dietary fibers, and detecting each index, wherein the results are shown in Table 4.
TABLE 4 Effect of different probiotics on Natural concentrated dietary fiber products
As can be seen from Table 4, under the same conditions of other preparation processes and with the same pine bark as raw materials, different probiotic fermentations have little influence on the crude fiber, NDF and ADF contents of the natural concentrated dietary fiber, but have obvious influence on the swellability and water retention capacity of the fiber, wherein the swellability and water retention capacity of the natural concentrated dietary fiber after the fermentation of the Lactobacillus plantarum HKB-38 are obviously higher than those of the Lactobacillus bulgaricus and the Streptococcus thermophilus.
Example 4: influence of high-temperature high-pressure heat treatment on water holding capacity of natural concentrated dietary fiber product
1) Cleaning Yunnan pine bark, removing impurities, crushing, sieving with a 60-mesh sieve, adding normal-temperature clear water, and soaking, wherein the ratio of the crushed raw materials to the normal-temperature clear water is 1 g: 20mL, soaking time is 10h, and waste water is drained.
2) Transferring the pine bark raw material obtained in the step 1) into a reactor, and adding clear water again, wherein the ratio of the pine bark raw material to the clear water at normal temperature is 1 g: 30mL, carrying out ultrasonic-assisted enzymolysis, specifically, after ultrasonic pretreatment for 10min, adding 2g of pectinase (the pectinase sold by Sigma in the United states and the product number of 17389) with the enzyme activity of 1.0U/mg and 10g of papain (the papain sold by Sigma in the United states and the product number of 76218) with the enzyme activity of 12U/mg into each liter of mixed solution for enzymolysis, wherein the enzymolysis temperature is 35 ℃, the enzymolysis pH value is 8.0, the enzymolysis time is 60min, and draining the liquid after enzymolysis to obtain the material after enzymolysis.
3) Inoculating lactobacillus plantarum HKB-38 into the material obtained in the step 2) after enzymolysis for fermentation, wherein the mass ratio of the lactobacillus plantarum HKB-38 to the material is 3 g: 1kg, fermentation temperature of 42 ℃ and fermentation time of 50min to obtain fermented materials.
4) Dividing the fermented material obtained in the step 3) into two equal parts, performing high-temperature and high-pressure heat treatment on one part at 360 ℃, under the pressure of 30MPa for 60min, cooling and drying to obtain natural concentrated dietary fiber; the other part is directly dried without high-temperature high-pressure heat treatment to obtain the natural concentrated dietary fiber, and the results are shown in table 5 after various indexes are detected.
TABLE 5 Effect of different probiotics on Natural concentrated dietary fiber products
As can be seen from table 5, under the same raw material and the same preparation method conditions, whether the high-temperature high-pressure heat treatment has little influence on the crude fiber, NDF and ADF content and fiber swelling property of the natural concentrated dietary fiber, but the water holding capacity of the natural concentrated dietary fiber is significantly improved after the high-temperature high-pressure heat treatment.
Therefore, the ultrasonic-assisted enzymatic treatment improves the enzymolysis efficiency, consumes less energy and enzyme, and better realizes the concentration of dietary fiber; the lactobacillus plantarum is used for fermentation, the fermentation effect is good, and the physiological properties of the dietary fiber such as fermentability, water holding capacity and expansibility are enhanced; the water holding capacity of the dietary fiber product is obviously improved through high-temperature and high-pressure heat treatment; in addition, the pine bark has low price and rich sources, and the production cost is greatly reduced.
In conclusion, the natural concentrated dietary fiber product prepared by the invention has the crude fiber content of more than or equal to 60 percent, the NDF content of more than or equal to 86 percent, the ADF content of more than or equal to 70 percent, the product swelling property of more than or equal to 5.0mL/g and the water holding capacity of more than or equal to 5.0 g/g.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any changes or substitutions that can be easily conceived by those skilled in the art within the technical scope of the present invention are included in the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the appended claims.
SEQUENCE LISTING
<110> Beijing Zhonghongke Biotechnology Co., Ltd, university of Hunan agriculture
<120> a natural concentrated dietary fiber and a method for preparing the same
<130>
<160> 1
<170> PatentIn version 3.5
<210> 1
<211> 1466
<212> DNA
<213> Lactobacillus plantarum
<400> 1
actggcggcg tgctatacat gcaagtcgaa cgaactctgg tattgattgg tgcttgcatc 60
atgatttaca tttgagtgag tggcgaactg gtgagtaaca cgtgggaaac ctgcccagaa 120
gcgggggata acacctggaa acagatgcta ataccgcata acaacttgga ccgcatggtc 180
cgagcttgaa agatggcttc ggctatcact tttggatggt cccgcggcgt attagctaga 240
tggtggggta acggctcacc atggcaatga tacgtagccg acctgagagg gtaatcggcc 300
acattgggac tgagacacgg cccaaactcc tacgggaggc agcagtaggg aatcttccac 360
aatggacgaa agtctgatgg agcaacgccg cgtgagtgaa gaagggtttc ggctcgtaaa 420
actctgttgt taaagaagaa catatctgag agtaactgtt caggtattga cggtatttaa 480
ccagaaagcc acggctaact acgtgccagc agccgcggta atacgtaggt ggcaagcgtt 540
gtccggattt attgggcgta aagcgagcgc aggcggtttt ttaagtctga tgtgaaagcc 600
ttcggctcaa ccgaagaagt gcatcggaaa ctgggaaact tgagtgcaga agaggacagt 660
ggaactccat gtgtagcggt gaaatgcgta gatatatgga agaacaccag tggcgaaggc 720
ggctgtctgg tctgtaactg acgctgaggc tcgaaagtat gggtagcaaa caggattaga 780
taccctggta gtccataccg taaacgatga atgctaagtg ttggagggtt tccgcccttc 840
agtgctgcag ctaacgcatt aagcattccg cctggggagt acggccgcaa ggctgaaact 900
caaaggaatt gacgggggcc cgcacaagcg gtggagcatg tggtttaatt cgaagctacg 960
cgaagaacct taccaggtct tgacatacta tgcaaatcaa gagattagac gttcccttcg 1020
gggacatgga tacaggtggt gcatggttgt cgtcagctcg tgtcgtgaga tgttgggtta 1080
agtcccgcaa cgagcgcaac ccttattatc agttgccagc attaagttgg gcactctggt 1140
gagactgccg gtgacaaacc ggaggaaggt ggggatgacg tcaaatcatc atgcccctta 1200
tgacctgggc tacacacgtg ctacaatgga tggtacaacg agttgcgaac tcgcgagagt 1260
aagctaatct cttaaagcca ttctcagttc ggattgtagg ctgcaactcg cctacatgaa 1320
gtcggaatcg ctagtaatcg cggatcagca tgccgcggtg aatacgttcc cgggccttgt 1380
acacaccgcc cgtcacacca tgagagtttg taacacccaa agtcggtggg gtaacctttt 1440
aggaaccagc cgctaagtga cagatt 1466
Claims (8)
1. Lactobacillus plantarum (II)Lactobacillus plantarum) HKB-38, deposited in China center for type culture Collection, CCTCC for short, at 2016, 12, month, 8, with the collection number of CCTCC NO: M2016727.
2. A natural concentrated dietary fiber is characterized in that pine bark rich in dietary fiber is crushed and then sieved, water is added for soaking, waste water is discharged after soaking is finished, then the soaked pine bark is transferred into a reactor, water is added to obtain pine bark mixed liquor, ultrasonic-assisted enzymolysis treatment is carried out, liquid is discharged after enzymolysis, then substrate materials after enzymolysis are inoculated into lactobacillus plantarum HKB-38 of claim 1 for fermentation, and finally the fermented substrate materials are subjected to high-temperature high-pressure heat treatment, cooled and dried to obtain the natural concentrated dietary fiber; the pine bark rich in dietary fiber is Yunnan pine bark, larch bark or masson pine bark, and the water is clear water at normal temperature.
3. The natural concentrated dietary fiber of claim 2, wherein the pine bark rich in dietary fiber is Yunnan pine bark.
4. A method of preparing a natural concentrated dietary fiber according to any one of claims 2 to 3, comprising the steps of:
1) cleaning pine bark raw materials rich in dietary cellulose, removing impurities, crushing, sieving with a 20-60-mesh sieve, and mixing the sieved pine bark raw materials with water according to a ratio of 1 g: 5-20 mL of the waste water is added with water for soaking for 2-10 hours, and waste water is discharged after soaking;
2) transferring the soaked pine bark obtained in the step 1) into a reactor, wherein the weight ratio of the soaked pine bark to normal-temperature clear water is 1 g: 10-30 mL of water is added to obtain pine bark mixed liquor, ultrasonic-assisted enzymolysis treatment is carried out, and after enzymolysis, liquid is drained to obtain an enzymolysis material;
3) inoculating probiotics into the material subjected to enzymolysis obtained in the step 2) for fermentation, and obtaining a fermented material from the material;
4) and (3) carrying out heat treatment on the fermented material obtained in the step 3) for 10-60 min at the temperature of 350-400 ℃ and the pressure of 5-30 MPa, cooling and drying to obtain the fermented material.
5. The preparation method of the natural concentrated dietary fiber according to claim 4, wherein in the step 2), the ultrasonic-assisted enzymolysis is performed by firstly performing ultrasonic treatment for 2-10 min, and then adding an enzyme preparation for enzymolysis, wherein the enzymolysis temperature is 35-55 ℃, the enzymolysis pH value is 6.0-8.0, and the enzymolysis time is 20-60 min.
6. The method for preparing natural concentrated dietary fiber according to claim 5, wherein the enzyme preparation is pectinase or/and papain; the enzyme activity of the pectinase is 0.5-5.0U/mg, and the ratio of the added amount of the pectinase to the pine bark mixed solution is 1-2 g: 1L; the enzyme activity of the papain is 5-50U/mg, and the ratio of the added amount of the papain to pine bark mixed liquor is 5-10 g: 1L of the compound.
7. The method for preparing natural concentrated dietary fiber according to claim 6, wherein the enzyme activity of the pectinase is 1.0U/mg and the enzyme activity of the papain is 12U/mg.
8. The preparation method of the natural concentrated dietary fiber according to any one of claims 4 to 7, wherein in the step 3), the mass ratio of the lactobacillus plantarum HKB-38 to the enzymolysis bottom material is 1-3 g: 1kg, the fermentation temperature is 35-42 ℃, and the fermentation time is 25-50 min.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711043437.3A CN107927321B (en) | 2017-10-31 | 2017-10-31 | Natural concentrated dietary fiber and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711043437.3A CN107927321B (en) | 2017-10-31 | 2017-10-31 | Natural concentrated dietary fiber and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107927321A CN107927321A (en) | 2018-04-20 |
| CN107927321B true CN107927321B (en) | 2021-05-18 |
Family
ID=61935937
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711043437.3A Active CN107927321B (en) | 2017-10-31 | 2017-10-31 | Natural concentrated dietary fiber and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107927321B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109601734A (en) * | 2018-12-28 | 2019-04-12 | 北京中农弘科生物技术有限公司 | Feed addictive containing procyanidine and dietary fiber and preparation method thereof and nonreactive pig starter feed product |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102864093A (en) * | 2012-04-18 | 2013-01-09 | 北京和美科盛生物技术有限公司 | Lactobacillus plantarum suitable for silage straw and use method thereof |
| CN105029452A (en) * | 2015-07-06 | 2015-11-11 | 青岛嘉瑞生物技术有限公司 | Method used for extracting dietary fiber from wheat straw via biological method |
| CN105124134A (en) * | 2015-07-21 | 2015-12-09 | 北京中农弘科生物技术有限公司 | Method of preparing feed dietary fibers |
| CN105533134A (en) * | 2015-12-16 | 2016-05-04 | 惠安县科联农业科技有限公司 | Preparation method of fermentation feed |
-
2017
- 2017-10-31 CN CN201711043437.3A patent/CN107927321B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102864093A (en) * | 2012-04-18 | 2013-01-09 | 北京和美科盛生物技术有限公司 | Lactobacillus plantarum suitable for silage straw and use method thereof |
| CN105029452A (en) * | 2015-07-06 | 2015-11-11 | 青岛嘉瑞生物技术有限公司 | Method used for extracting dietary fiber from wheat straw via biological method |
| CN105124134A (en) * | 2015-07-21 | 2015-12-09 | 北京中农弘科生物技术有限公司 | Method of preparing feed dietary fibers |
| CN105533134A (en) * | 2015-12-16 | 2016-05-04 | 惠安县科联农业科技有限公司 | Preparation method of fermentation feed |
Also Published As
| Publication number | Publication date |
|---|---|
| CN107927321A (en) | 2018-04-20 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN110438028B (en) | Min pig source Bacillus beleisi GX-1 for degrading cellulose | |
| JP5249817B2 (en) | Immunostimulated fermented food from fructan-containing food | |
| CN110331109B (en) | Bacillus subtilis and culture method and application thereof | |
| CN1769424A (en) | Bacillus strain and its uses | |
| CN102212479B (en) | Method for pretreating beer waste for biochemical utilization | |
| CN111642622A (en) | Preparation and application of fermentation composite bacteria and myrtle fermentation extract | |
| CN113980848A (en) | Pediococcus pentosaceus SBC5 and application thereof | |
| CN114107403B (en) | Method for co-producing ellagic acid and biological feed by fermenting pericarpium Granati with microbial community | |
| CN108486002B (en) | Momordica grosvenori endophyte strain capable of producing exopolysaccharides, method for producing exopolysaccharides and application of exopolysaccharides | |
| CN107927321B (en) | Natural concentrated dietary fiber and preparation method thereof | |
| CN114214247A (en) | Bacillus licheniformis and application thereof | |
| CN104357364A (en) | Streptomycete strain and method for preparing alkali-resistant salt-resistant xylanase by using same | |
| CN115418337B (en) | Lignin degrading bacterium and application thereof in rice straw micro-storage | |
| CN112869179A (en) | Process for extracting dietary fiber from sweet potatoes | |
| CN111466480A (en) | Tea residue and tea polysaccharide probiotic fermented feed and preparation method thereof | |
| CN114540232B (en) | Lactobacillus rhamnosus with aquatic pathogen antagonistic property and preparation and application of lactobacillus rhamnosus preparation | |
| CN108823266A (en) | A kind of method that the method using fermentation prepares chitin | |
| CN109136303A (en) | A kind of preparation method of the seaweed diet fiber rich in algin oligosaccharide | |
| CN108239616A (en) | One Enterococcus faecalis bacterial strain and its application in wintercherry Tofu processing | |
| CN108330087B (en) | Solid leaven for fermenting peanut straw | |
| CN110592047A (en) | Novel method for producing feruloyl esterase by fermenting straws with Verbena pyricularis and application | |
| CN111700279A (en) | Preparation method of wheat bran dietary fiber | |
| CN117210365B (en) | Bacillus bailii and application thereof in improving digestion and antioxidation capability | |
| CN114437969B (en) | Lactobacillus acidophilus MD-286 and application thereof | |
| CN114591863B (en) | Tibetan pig source fungicide combination for improving growth performance and immune function of weaned piglets |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |