CN106957872A - A kind of method that fixing lactic acid bacteria prepares cigarette Semen Armeniacae Amarum extract - Google Patents
A kind of method that fixing lactic acid bacteria prepares cigarette Semen Armeniacae Amarum extract Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
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- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
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- C12R2001/25—Lactobacillus plantarum
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Abstract
The present invention provides a kind of method that fixing lactic acid bacteria prepares cigarette Semen Armeniacae Amarum extract.The preparation method is concretely comprised the following steps：(1) semen armeniacae amarae water extraction at 95~100 DEG C is taken repeatedly, to merge extract solution, obtain Semen Armeniacae Amarum extract A；(2) using lactobacillus inoculum cultivate into broth bouillon centrifugation after obtain wet thallus, in the mixed solution that wet thallus is added to polyvinyl alcohol and sodium alginate, and with contain 1~3%CaCl2Boric acid saturated solution and saturation Na2SO4PVA SA fixing lactic acid bacteria beads are crosslinked to obtain in solution；(3) by Semen Armeniacae Amarum extract A and fixing lactic acid bacteria bead according to mass ratio 5~10:1 mixing after fermentation, then filters to obtain clarified broth, adds alcoholic solution precipitation, filters to take supernatant concentration and obtain cigarette Semen Armeniacae Amarum extract.The present invention, which is added in cigarette, increases the fragrant note of semen armeniacae amarae feature note, acid, lifts cigarette flavor matter, the increase cigarette flavor property sent out thoroughly, and increase flue gas promotes the production of body fluid sense, and soft fine and smooth flue gas reduces excitant, and improvement throat comfortableness covers miscellaneous gas.
Description
Technical field
The present invention relates to cigarette additive field, and in particular to prepares cigarette Semen Armeniacae Amarum extract using fixing lactic acid bacteria
Preparation method.
Background technology
Flavoring and casing is the key link in cigarette process, is the determinant and raising cigarette flavor for improving product style
One of effective means of quality.Current essence spice for cigarette is main or passes through the extraction to natural animal-plant, chemical synthesis system
It is standby.Due to by natural plant resource is limited and the harsher limitation of chemical synthesis condition.With the development of biotechnology, in recent years
Come, prepare cigarette flavor using microbe fermentation method, an economy, efficient approach are provided for the production of cigarette flavor,
Develop the new cigarette flavor that some conventional methods can not be produced.
Immobilized microorganism technology, is to be fixed on special microorganism on the carrier of choosing card, makes its highly dense and protect
Bioactivity is held, the biotechnology that can quickly, largely breed under condition of compatibility can make up suspension microorganism fermentation process
The shortcomings of efficiency is low, separation of solid and liquid is difficult, mithridatism is poor.Therefore, in recent years, the research of immobilized microorganism technology is lived very much
Jump, development is very fast, has application to many industrial departments such as food fermentation industry, environmental protection, new energy development and biological biography
Sensor etc., and deep development is achieved, show powerful superiority.It is mainly concerned with the following aspects：In food hair
It is used to produce ethanol, beer, Yoghourt etc. in ferment industry；It is used for environmental monitoring and the processing of industrial wastewater in environmental protection；
It is used to produce hydrogen, methane, biochemical fuel cell, microorganism battery etc. in new energy development；Immobilized microorganism is alternatively arranged as molecule
Recognition component, for making various biology sensors.And it is continuous ripe, perfect with fixation of microbe technology, gradually to height
The direction such as effect low energy consumption, low cost, simplification, safety automation, pollution-free is developed, in energy crisis, shortage of resources, environment
In the case that pollution is on the rise, it thus will be promoted more to go deep into and be widely applied in terms of wastewater treatment, with more
Profound significance.Especially in terms of wastewater treatment, have great application prospect.But prepared using immobilized microorganism technology
Cigarette but has no report with Semen Armeniacae Amarum extract.
Semen armeniacae amarae, alias almond (scientific name：Semen Armeniacae Amarum.) be rosaceous plant ansu apricot drying
Mature seed.Summer harvests ansu apricot ripening fruits, removes pulp and nucleocapsid, takes out seed and dries, semen armeniacae amarae is made.Mainly originate in
The ground such as the Inner Mongol, Jilin, Liaoning, Hebei, Shanxi, Shaanxi.Semen armeniacae amarae, which cures mainly function, has sending down abnormally ascending relieving cough and asthma, relaxes bowel.With
In treatment cough and asthma, fullness sensation in chest abundant expectoration, deficiency of blood Tianjin is withered, the illness such as dry constipation of intestines.Semen armeniacae amarae medicinal extract by raw material of semen armeniacae amarae, hardship
Apricot seed extract is widely used in cigarette, with abundant cigarette perfume, increases the effect of semen armeniacae amarae feature note.
Lactic acid bacteria (lactic acid bacteria, LAB) is that a class can be produced largely using fermentable carbohydrate
The common name of the bacterium of lactic acid.The bacterium can not only produce the irritating and soft fine and smooth flue gas effect of reduction cigarette during the fermentation
Lactic acid, moreover it is possible to produce assign the peculiar style of cigarette flavor substance.
The content of the invention
It is an object of the invention to provide a kind of method that fixing lactic acid bacteria prepares cigarette Semen Armeniacae Amarum extract, using fixation
Change lactobacillus-fermented technology, develop a kind of new Semen Armeniacae Amarum extract, the extract can be used in cigarette, Neng Goufeng
Rich cigarette perfume, increase semen armeniacae amarae feature note, fragrant and sweet note and the fragrant note of acid, lifts cigarette flavor matter, and increase cigarette flavor is sent out thoroughly
Property, increase flue gas promotes the production of body fluid sense, and soft fine and smooth flue gas reduces excitant, and improvement throat comfortableness covers miscellaneous gas.
The present invention provides a kind of method that fixing lactic acid bacteria prepares cigarette Semen Armeniacae Amarum extract, it is characterised in that specific bag
Include following steps：
(1) semen armeniacae amarae is taken, the water of 2~5 times of its weight is added, 12-24h, the refluxing extraction 2~3 at 95~100 DEG C is soaked
Secondary, each extraction time is 5~8 hours, and room temperature is cooled to after having extracted, and is filtrated to get extract solution, merges multiple extract solution,
Obtain Semen Armeniacae Amarum extract A；
(2) by lactobacillus inoculum into broth bouillon, cultivated under conditions of 37 DEG C of temperature, speed of agitator are 50rpm
48 hours, take nutrient solution in being centrifuged under 8000rpm, the wet thallus after centrifugation is standby；
(3) polyvinyl alcohol-Immobilization in Sodium Alginate of lactic acid bacteria：It is water-soluble with sodium alginate using polyvinyl alcohol water solution
Liquid prepares the polyvinyl alcohol containing mass percent 6%~10% and the mixing of the sodium alginate of mass percent 1%~3% is molten
The lactic acid bacteria wet thallus prepared in liquid, the step of the 2~5% of total solution volume are then added into mixed solution (2), is configured to
Mixed liquor, CaCl is added dropwise to peristaltic pump by the syringe needle in 1~5mm apertures by the mixed liquor prepared2Quality percentage
Than being crosslinked 1h formation beads in the boric acid saturated solution for 1~3%, then bead is taken out and adds saturation Na2SO4In solution
3-5h is crosslinked, PVA-SA fixing lactic acid bacteria beads, finally using aseptic water washing, dry for standby is obtained；
(4) by the fixing lactic acid bacteria bead prepared in Semen Armeniacae Amarum extract A and step (3) according to mass ratio 5~10:1
Mixing, is 30~37 DEG C in temperature, and speed of agitator is 3~5d of fermentation under conditions of 1~5rpm, is subsequently placed in quiet in 4 DEG C of refrigerators
Put 7~14d；
(5) step (4) fermentation after fermentation liquid is filtered, obtains clarified broth, zymotic fluid is freeze-dried, feed liquid thickness
For 1~2 centimetre, vacuum is 10Pa, and drying time is 6~12h；
(6) zymotic fluid after step (5) is freeze-dried, adds 80~95% alcoholic solutions of 2~4 times of its quality, sinks
Form sediment 24~48 hours, it is 0.1MPa to filter to take supernatant in pressure, and temperature is to be concentrated into relative density under conditions of 45 DEG C to be
1.20~1.25, obtain Semen Armeniacae Amarum extract.
The lactic acid bacteria used in the present invention is Lactobacillus plantarum (Lactobacillus plantarum) PJH05, in 2012
On January 5, in is preserved in Wuhan University's China typical culture collection center, and preserving number is：CCTCC NO：M2012002.
The immobilized microorganism technology that the present invention is used has that fermentation process efficiency high, separation of solid and liquid be easy, mithridatism is strong
The advantages of, the deficiency of traditional biological technique can be made up.
The present invention, using fixing lactic acid bacteria fermentation Semen Armeniacae Amarum extract, obtains a kind of quality using semen armeniacae amarae as raw material
Excellent cigarette Semen Armeniacae Amarum extract, the extract has abundant cigarette perfume in cigarette, and increase semen armeniacae amarae feature note, increase are sweet
Note, lifting cigarette flavor matter, increase the cigarette flavor property sent out thoroughly, increase flue gas promote the production of body fluid sense, soft fine and smooth flue gas, reduction stimulation
Property, improve throat's comfortableness, cover the effect such as miscellaneous gas, moreover it is possible to assign the sour fragrant breeze lattice of cigarette characteristic.With existing semen armeniacae amarae medicinal extract
Compared with Semen Armeniacae Amarum extract, the Semen Armeniacae Amarum extract reduces sharp property in cigarette and the effect of soft fine and smooth flue gas is more apparent,
The sour fragrant breeze lattice of cigarette characteristic are also assigned simultaneously；Compared with suspension lactobacillus-fermented, Semen Armeniacae Amarum extract prepared by this method has
Machine acid content is added in 2.25%~6.38%, cigarette effect assessment, and oral cavity pleasant impression is more comfortable, and sense of promoting the production of body fluid is more preferable, fragrance
More enrich.This method instead of the traditional extractive technique of Semen Armeniacae Amarum extract, have updated biofermentation preparation technology so that its
Applicability it can increase in cigarette, the preparation to cigarette Semen Armeniacae Amarum extract provides a new way.
Embodiment
With reference to embodiment, the invention will be further described.
The lactic acid bacteria used in the present invention is Lactobacillus plantarum (Lactobacillus plantarum) PJH05, in 2012
On January 5, in is preserved in Wuhan University's China typical culture collection center, and preserving number is：CCTCC NO：M2012002.
PVA-SA (polyvinyl alcohol-sodium alginate) fixing lactic acid bacteria bead in following examples of the present invention be according to
Lower method is obtained using polyvinyl alcohol (PVA) and sodium alginate (SA) to being fixed of lactic acid bacteria：
(1) by lactobacillus inoculum into broth bouillon, cultivated under conditions of 37 DEG C of temperature, speed of agitator are 50rpm
48 hours, take nutrient solution in being centrifuged under 8000rpm, the wet thallus after centrifugation is standby；It is conventional that agar culture is supported in the above method
Broth medium, its Ju Ti Pei Fang be (every liter)：Peptone 10g, beef extract powder 3g, sodium chloride 5g, final pH 7.3 ±
0.1；
(2) polyvinyl alcohol-Immobilization in Sodium Alginate of lactic acid bacteria：It is water-soluble with sodium alginate using polyvinyl alcohol water solution
Liquid prepares the mixed solution of the polyvinyl alcohol of the 6%-10% containing mass percent and mass percent 1%-3% sodium alginate,
Then the lactic acid bacteria wet thallus prepared in the step of 2-5% of total solution volume is added into mixed solution (2), is configured to mixing
Liquid, CaCl is added dropwise to peristaltic pump by the syringe needle in 1-5mm apertures by the mixed liquor prepared2Mass percent be 1-
In 3% boric acid saturated solution, then bead is taken out and adds saturation Na by crosslinking 1h formation beads2SO43- is crosslinked in solution
5h, obtains PVA-SA fixing lactic acid bacteria beads, finally using aseptic water washing, dry for standby；It is described to contain 1-3%CaCl2's
The amount of boric acid saturated solution and and saturation Na2SO4The two of solution contain 1-3%CaCl without particular/special requirement2Boric acid saturated solution
Only need to that mixed liquor can be made to produce precipitation, meet the immobilization requirement of lactic acid bacteria, Na2SO4Solution is only needed to bead
It is completely soaked and just may be used；The boric acid saturated solution and saturation Na2SO4Solution is saturated aqueous solution.
Embodiment 1, a kind of method that fixing lactic acid bacteria prepares cigarette Semen Armeniacae Amarum extract, comprises the following steps：
(1) add 5 times of water by weight into semen armeniacae amarae, soak 24h, refluxing extraction 2 times at 100 DEG C are extracted every time
Time is 5 hours, and room temperature is cooled to after having extracted, and is filtrated to get extract solution, merges extract solution twice, obtains semen armeniacae amarae extraction
Thing A, the condition extracted twice is identical；
(2) the PVA-SA fixing lactic acid bacterias bead for preparing Semen Armeniacae Amarum extract A and the above method is according to mass ratio 5:1
Mixing, is 30 DEG C in temperature, speed of agitator is the 4d that fermented under conditions of 5rpm, is subsequently placed in 4 DEG C of refrigerators and stands 7d；
(3) step (2) fermentation after fermentation liquid is filtered, obtains clarified broth, zymotic fluid is freeze-dried, feed liquid thickness
For 1~2 centimetre, vacuum is 10Pa, and drying time is 9h；
(4) zymotic fluid after step (3) freeze-drying is taken, 80~95% alcoholic solutions of 2 times of its quality, precipitation 24 are added
Hour, it is 0.1MPa to filter to take supernatant in pressure, and temperature is that to be concentrated into relative density under conditions of 45 DEG C be 1.20, obtains bitter apricot
Benevolence extract.
The Semen Armeniacae Amarum extract prepared in embodiment 1 has an abundant cigarette perfume in cigarette, increase semen armeniacae amarae feature note, sweet
Note and the fragrant note of acid, lift cigarette flavor matter, the increase cigarette flavor property sent out thoroughly, and increase flue gas promotes the production of body fluid sense, soft fine and smooth cigarette
Gas, reduces excitant, improves throat's comfortableness, covers miscellaneous gas.
Embodiment 2, a kind of method that fixing lactic acid bacteria prepares cigarette Semen Armeniacae Amarum extract, comprises the following steps：
(1) take semen armeniacae amarae to spend, add the water of 8 times of its weight, soak 12h, then refluxing extraction 3 times at 95 DEG C, every time
Extraction time is 8 hours, and room temperature is cooled to after having extracted, and is filtrated to get extract solution, merges No. three extract solutions, obtains semen armeniacae amarae
Extract A；The condition of three extractions is identical；
(2) the PVA-SA fixing lactic acid bacteria beads that will be prepared in Semen Armeniacae Amarum extract A and the fixation above method prepared
According to mass ratio 8:1 mixing, is 33 DEG C in temperature, speed of agitator is the 3d that fermented under conditions of 4rpm, is subsequently placed in 4 DEG C of refrigerators
Stand 12d；
(3) step (2) fermentation after fermentation liquid is filtered, obtains clarified broth, zymotic fluid is freeze-dried, feed liquid thickness
For 1~2 centimetre, vacuum is 10Pa, and drying time is 12h；
(4) zymotic fluid after step (3) freeze-drying is taken, 80~95% alcoholic solutions of 2 times of its quality, precipitation 36 are added
Hour, it is 0.1MPa to filter to take supernatant in pressure, and temperature is that to be concentrated into relative density under conditions of 45 DEG C be 1.20, obtains bitter apricot
Benevolence extract.
The Semen Armeniacae Amarum extract prepared in embodiment 2 has an abundant cigarette perfume, increase semen armeniacae amarae feature note, fragrant and sweet note and
Sour perfume note, lifts cigarette flavor matter, the increase cigarette flavor property sent out thoroughly, and increase flue gas promotes the production of body fluid sense, and soft fine and smooth flue gas, reduction is pierced
Swash property, improve throat's comfortableness, cover miscellaneous gas.
Embodiment 3, a kind of method that fixing lactic acid bacteria prepares cigarette Semen Armeniacae Amarum extract, comprises the following steps：
(1) semen armeniacae amarae is taken, the water of 5 times of its weight is added, soaked 18 hours, then refluxing extraction 3 times at 98 DEG C, every time
Extraction time is 7 hours, and room temperature is cooled to after having extracted, and is filtrated to get extract solution, merges No. three extract solutions, obtains semen armeniacae amarae
Extract A；Three times extraction conditions are identical；
(2) the PVA-SA fixing lactic acid bacteria beads for preparing Semen Armeniacae Amarum extract A and the above method are according to mass ratio
10:1 mixing, is 36 DEG C in temperature, speed of agitator is the 5d that fermented under conditions of 2rpm；
(3) step (2) fermentation after fermentation liquid is filtered, obtains clarified broth, zymotic fluid is freeze-dried, feed liquid thickness
For 1~2 centimetre, vacuum is 10Pa, and drying time is 7h；
(4) zymotic fluid after step (3) freeze-drying is taken, 80~95% alcoholic solutions of 2 times of its quality, precipitation 24 are added
Hour, it is 0.1MPa to filter to take supernatant in pressure, and temperature is that to be concentrated into relative density under conditions of 45 DEG C be 1.20, obtains bitter apricot
Benevolence extract.
The Semen Armeniacae Amarum extract prepared in embodiment 3 has an abundant cigarette perfume, increase semen armeniacae amarae feature note, fragrant and sweet note and
Sour perfume note, lifts cigarette flavor matter, the increase cigarette flavor property sent out thoroughly, and increase flue gas promotes the production of body fluid sense, and soft fine and smooth flue gas, reduction is pierced
Swash property, improve throat's comfortableness, cover miscellaneous gas.
Contrast experiment, the bitter apricot that the Semen Armeniacae Amarum extract that applicant prepares the present invention is prepared with existing traditional extraction process
Benevolence extract, the Semen Armeniacae Amarum extract prepared using suspension lactobacillus-fermented are added in pipe tobacco according to normal perfuming mode, are made
Into cigarette, and smoked panel test, its effect of smokeing panel test is as follows：
The sensory evaluation effect that semen armeniacae amarae prepared by three kinds of different process is extracted in cigarette compares
It can be seen that to come from above-mentioned contrast experiment, the Semen Armeniacae Amarum extract for preparing is added in cigarette effect more in the present invention
It is good, and found according to specific experiment analysis, compared with suspension lactobacillus-fermented, Semen Armeniacae Amarum extract prepared by this method is organic
Acid content is added in 2.25%~6.38%, cigarette effect assessment, and oral cavity pleasant impression is more comfortable, and fragrance more enriches.
Claims (2)
1. a kind of method that fixing lactic acid bacteria prepares cigarette Semen Armeniacae Amarum extract, it is characterised in that specifically include following steps：
(1) semen armeniacae amarae is taken, the water of 2~5 times of its weight is added, 12-24h is soaked, refluxing extraction 2~3 times at 95~100 DEG C,
Each extraction time is 5~8 hours, and room temperature is cooled to after having extracted, and is filtrated to get extract solution, merges multiple extract solution, obtains
To Semen Armeniacae Amarum extract A；
(2) by lactobacillus inoculum into broth bouillon, culture 48 is small under conditions of 37 DEG C of temperature, speed of agitator are 50rpm
When, take nutrient solution in being centrifuged under 8000rpm, the wet thallus after centrifugation is standby；
(3) polyvinyl alcohol-Immobilization in Sodium Alginate of lactic acid bacteria：Matched somebody with somebody using polyvinyl alcohol water solution with sodium alginate aqueous solution
The mixed solution of the polyvinyl alcohol containing mass percent 6%~10% and the sodium alginate of mass percent 1%~3% is made, so
The lactic acid bacteria wet thallus prepared in the step of adding the 2~5% of total solution volume in backward mixed solution (2), is configured to mixing
Liquid, CaCl is added dropwise to peristaltic pump by the syringe needle in 1~5mm apertures by the mixed liquor prepared2Mass percent be 1
1h formation beads are crosslinked in~3% boric acid saturated solution, then bead is taken out and adds saturation Na2SO43- is crosslinked in solution
5h, obtains PVA-SA fixing lactic acid bacteria beads, finally using aseptic water washing, dry for standby；
(4) by the fixing lactic acid bacteria bead prepared in the Semen Armeniacae Amarum extract A and step (3) that are prepared in step (1) according to matter
Measure ratio 5~10:1 mixing, is 30~37 DEG C in temperature, and speed of agitator is 3~5d of fermentation under conditions of 1~5rpm, is subsequently placed in 4
7~14d is stood in DEG C refrigerator；
(5) filtering fermentation liquor after step (4) fermentation refrigeration is taken, clarified broth is obtained, zymotic fluid is freeze-dried, feed liquid is thick
Spend for 1~2 centimetre, vacuum is 10Pa, drying time is 6~12h；
(6) zymotic fluid after being freeze-dried in step (5) is taken, 80~95% alcoholic solutions of 2~4 times of its quality are added, precipitated
24~48 hours, it was 0.1MPa to filter to take supernatant in pressure, and temperature is that to be concentrated into relative density under conditions of 45 DEG C be 1.20
~1.25, obtain Semen Armeniacae Amarum extract.
2. the method that a kind of fixing lactic acid bacteria according to claim 1 prepares cigarette Semen Armeniacae Amarum extract, its feature exists
Lactic acid bacteria in the step (2) is Lactobacillus plantarum (Lactobacillus plantarum) PJH05CCTCC NO：
M2012002。
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109439442A (en) * | 2018-12-20 | 2019-03-08 | 湖北中烟工业有限责任公司 | A kind of apricot flavor essence and its preparation method and application for cigarette dripping pill |
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| CN105062677A (en) * | 2015-07-31 | 2015-11-18 | 湖北中烟工业有限责任公司 | Method for preparing fermented beet extract through processing beet juice by Lactobacillus plantarum |
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| CN1158734A (en) * | 1996-12-27 | 1997-09-10 | 张中营 | Cigariete-type medicine for curing tracheitis and asthma and its production process |
| CN102505036A (en) * | 2011-11-23 | 2012-06-20 | 天津科技大学 | Method for improving monascus pigment color value by using immobilized lactic acid bacteria |
| CN105062677A (en) * | 2015-07-31 | 2015-11-18 | 湖北中烟工业有限责任公司 | Method for preparing fermented beet extract through processing beet juice by Lactobacillus plantarum |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109439442A (en) * | 2018-12-20 | 2019-03-08 | 湖北中烟工业有限责任公司 | A kind of apricot flavor essence and its preparation method and application for cigarette dripping pill |
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