CN106957863A - A kind of method that immobilized yeast prepares cigarette black cloth woods extract - Google Patents

A kind of method that immobilized yeast prepares cigarette black cloth woods extract Download PDF

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CN106957863A
CN106957863A CN201610895931.1A CN201610895931A CN106957863A CN 106957863 A CN106957863 A CN 106957863A CN 201610895931 A CN201610895931 A CN 201610895931A CN 106957863 A CN106957863 A CN 106957863A
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black cloth
cloth woods
extract
cigarette
immobilized yeast
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庞登红
黄龙
王萍
易建云
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Wuhan Huanghelou Flavoring and Essence Co Ltd
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Wuhan Huanghelou Flavoring and Essence Co Ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
    • C12P1/02—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using fungi
    • A—HUMAN NECESSITIES
    • A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
    • A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
    • A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
    • A24B15/18—Treatment of tobacco products or tobacco substitutes
    • A24B15/24—Treatment of tobacco products or tobacco substitutes by extraction; Tobacco extracts
    • A—HUMAN NECESSITIES
    • A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
    • A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
    • A24B3/00—Preparing tobacco in the factory
    • A24B3/12—Steaming, curing, or flavouring tobacco
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/18—Baker's yeast; Brewer's yeast
    • C12N1/185—Saccharomyces isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/04—Enzymes or microbial cells immobilised on or in an organic carrier entrapped within the carrier, e.g. gel or hollow fibres
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/10—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a carbohydrate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/85—Saccharomyces
    • C12R2001/865—Saccharomyces cerevisiae

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
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  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
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  • General Chemical & Material Sciences (AREA)
  • Dispersion Chemistry (AREA)
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  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
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Abstract

The present invention provides a kind of method that immobilized yeast prepares cigarette black cloth woods extract, comprises the following steps：(1) fresh black cloth woods are taken, are squeezed the juice, (2) prepare saccharomycete bacteria suspension, and (3) are using sodium alginate CaCl2By yeast bacteria immobilization, immobilized yeast and black cloth woods juice are carried out fermentation reaction by (4), and filtering fermentation liquor is obtained clarified broth by (5), by alcohol precipitation, and black cloth woods extract is obtained after being concentrated under reduced pressure.The present invention is using black cloth woods as raw material, utilize immobilized yeast fermentation black cloth woods juice, obtain a kind of cigarette best in quality black cloth woods extract, the extract has abundant cigarette perfume in cigarette, saturating smoke is fragrant, the fragrant and sweet rhythm of increase fruit, and increase flue gas promotes the production of body fluid sense, soft fine and smooth flue gas, mitigates the effect such as throat's excitant.

Description

A kind of method that immobilized yeast prepares cigarette black cloth woods extract
Technical field
The present invention relates to cigarette additive field, and in particular to prepares cigarette black cloth woods extract using immobilized yeast Method.
Background technology
Flavoring and casing is the key link in cigarette process, is the determinant and raising cigarette flavor for improving product style One of effective means of quality.Current essence spice for cigarette is main or passes through the extraction to natural animal-plant, chemical synthesis system It is standby.Due to by natural plant resource is limited and the harsher limitation of chemical synthesis condition.With the development of biotechnology, in recent years Come, prepare cigarette flavor using microbe fermentation method, an economy, efficient approach are provided for the production of cigarette flavor, Develop the new cigarette flavor that some conventional methods can not be produced.
Immobilized microorganism technology, is to be fixed on special microorganism on the carrier of choosing card, makes its highly dense and protect Bioactivity is held, the biotechnology that can quickly, largely breed under condition of compatibility can make up suspension microorganism fermentation process The shortcomings of efficiency is low, separation of solid and liquid is difficult, mithridatism is poor.Therefore, in recent years, the research of immobilized microorganism technology is lived very much Jump, development is very fast, has application to many industrial departments such as food fermentation industry, environmental protection, new energy development and biological biography Sensor etc., and deep development is achieved, show powerful superiority.It is mainly concerned with the following aspects：In food hair It is used to produce ethanol, beer, Yoghourt etc. in ferment industry；It is used for environmental monitoring and the processing of industrial wastewater in environmental protection； It is used to produce hydrogen, methane, biochemical fuel cell, microorganism battery etc. in new energy development；Immobilized microorganism is alternatively arranged as molecule Recognition component, for making various biology sensors.And it is continuous ripe, perfect with fixation of microbe technology, gradually to height The direction such as effect low energy consumption, low cost, simplification, safety automation, pollution-free is developed, dirty in energy crisis, shortage of resources, environment In the case that dye is on the rise, it thus will be promoted more deeply and to be widely applied in terms of wastewater treatment, with more deepening Remote meaning.Especially in terms of wastewater treatment, have great application prospect.But preparing cigarette using immobilized microorganism technology Report is but had no with black cloth woods extract.
Black cloth woods (scientific name：Myrica rubra (Lour.) S.et Zucc.) it is rose family Prunus arbor, also known as black Lee Son, black cloth are bright etc., and because the kind is introduced from the U.S., the color of fruit is atropurpureus, and American Black Lee, U.S. Lee are called again.With Black cloth woods are that black cloth woods medicinal extract prepared by raw material applies also wide in cigarette, have abundant cigarette perfume, increase fruit in cigarette Fragrant and sweet rhythm etc. is acted on.
Saccharomycete is some unicellular fungis, is sac fungus, the common name of basidiomycetes Deng Ji sections unicellular fungi, typically refers to The fungi of energy fermenting carbohydrate, available for production is brewageed, the bacterium can produce the fragrance thing such as ethyl acetate, benzyl carbinol during the fermentation Matter, these aroma substances can assign the fragrant and sweet fragrance of cigarette feature, be a kind of high-quality aroma former for producing tobacco aromaticss.
The content of the invention
It is an object of the invention to provide a kind of method that immobilized yeast prepares cigarette black cloth woods extract, fixation is utilized Change microbial technique, develop a kind of new black cloth woods extract.
The present invention provides a kind of method that immobilized yeast prepares cigarette black cloth woods extract, comprises the following steps：
(1) fresh black cloth woods are taken, fruit stone is removed, squeezes the juice, sterilize 20min at 121 DEG C, cools down, is filtrated to get black cloth woods juice；
(2) saccharomycete bacteria suspension is prepared：Saccharomycete is inoculated into broth bouillon, is in 37 DEG C of temperature, speed of agitator 48h is cultivated under conditions of 50rpm, takes nutrient solution in being centrifuged under 8000rpm, the wet thallus after centrifugation is standby；
(3) sodium alginate-CaCl of saccharomycete2Immobilization：The sodium alginate soln that mass fraction is 3%~6% is prepared, Then the saccharomycete wet thallus prepared in 3%~5% step (2) of its volume is added, mixed liquor is configured to, with wriggling The mixed liquor prepared is added dropwise to the CaCl that mass fraction is 3%~6% by pump by the syringe needle in 1~5mm apertures2Solution In, balling-up, and by CaCl2Solution and bead be stored in -4 DEG C under the conditions of 12~24h, take out bead, with aseptic water washing, drying It is standby；
(4) by the immobilized yeast bead prepared in black cloth woods juice and step (3) according to mass ratio 2:1~5:1 mixing, It it is 20~28 DEG C in temperature, speed of agitator is 48~96h of fermentation under conditions of 1~5rpm；It is subsequently placed in 10 DEG C of refrigerators and preserves 4 ~7d, then in 12~24h of placement under normal temperature；
(5) ferment after fermentation liquid of step (4) is filtered, obtains clarified broth, add 2~4 times of its quality 80%~ 95% alcoholic solution, precipitates 24~48h, and it is 1.25~1.35 to filter to take supernatant and be concentrated under reduced pressure into relative density, obtains black cloth woods Extract.
The saccharomycete used in the present invention is saccharomyces cerevisiae (Saccharomyces cerevisiae) HHL-1, in 2009 It is preserved in Wuhan University's China typical culture collection center on November 17, in, deposit number is CCTCC NO:M 209272.
Further, the Ju Ti Pei Fang of broth bouillon is (every liter) in the step (2)：Peptone 10g, beef extract powder 3g, sodium chloride 5g, final pH 7.3 ± 0.1.
Further, the condition being concentrated under reduced pressure in the step (5)：Concentrate pressure 0.1MPa, 55 DEG C of thickening temperature.
The immobilized microorganism technology that the present invention is used has that fermentation process efficiency high, separation of solid and liquid be easy, mithridatism is strong The advantages of, the deficiency of traditional biological technique can be made up.
The present invention, using immobilized yeast fermentation black cloth woods extract, obtains a kind of quality using black cloth woods as raw material Excellent cigarette black cloth woods extract, the extract has abundant cigarette perfume in cigarette, and saturating smoke is fragrant, and the fragrant and sweet rhythm of increase fruit increases Smoke adding gas promotes the production of body fluid sense, soft fine and smooth flue gas, mitigates the effect such as throat's excitant.With existing black cloth woods medicinal extract and black cloth woods extract Compare, the black cloth woods extract in cigarette fragrance more enrich, fruity rhythm and fragrant and sweet rhythm are more obvious, fragrance thoroughly the property sent out compared with It is good, excitant reduction；Compared with suspended yeast bacterium is fermented, esters fragrance content of material in black cloth woods extract prepared by this method 13.58%~17.29% is added, benzyl carbinol content is added in 9.27%~13.54%, cigarette effect assessment, and fragrance is more Plus enrich, send out thoroughly, the fragrant and sweet rhythm of fruit is more obvious.This method instead of the traditional extractive technique of black cloth woods extract, have updated life Thing fermentation preparation technology so that it applicability can increase in cigarette, one is provided to cigarette with the preparation of black cloth woods extract Bar new way.
Embodiment
With reference to embodiment to further detailed description of the present invention.
Embodiment 1
(1) fresh black cloth woods are taken, fruit stone is removed, squeezes the juice, sterilize 20min at 121 DEG C, cools down, is filtrated to get black cloth woods juice；
(2) saccharomycete bacteria suspension is prepared：Saccharomycete is inoculated into broth bouillon, the Ju Ti Pei Fang of broth bouillon is (every liter)：Peptone 10g, beef extract powder 3g, sodium chloride 5g, final pH 7.3 ± 0.1 is in 37 DEG C of temperature, speed of agitator 48h is cultivated under conditions of 50rpm, takes nutrient solution in being centrifuged under 8000rpm, the wet thallus after centrifugation is standby；
(3) sodium alginate-CaCl of saccharomycete2Immobilization：The sodium alginate soln that mass fraction is 4% is prepared, then The saccharomycete wet thallus prepared in 3% step (2) of its volume is added, mixed liquor is configured to, passes through 1mm with peristaltic pump The mixed liquor prepared is added dropwise to the CaCl that mass fraction is 3% by the syringe needle in aperture2In solution, balling-up, and by CaCl2 Solution and bead be stored in -4 DEG C under the conditions of 16h, take out bead, with aseptic water washing, dry for standby；
(4) by the immobilized yeast bead prepared in black cloth woods juice and step (3) according to mass ratio 2:1 mixing, in temperature Spend for 20 DEG C, speed of agitator is the 50h that fermented under conditions of 5rpm；It is subsequently placed in 10 DEG C of refrigerators and preserves 4d, then under normal temperature Place 12h；
(5) step (4) fermentation after fermentation liquid is filtered, obtains clarified broth, 80% alcohol for adding 2 times of its quality is molten Liquid, precipitates 28h, filters to take supernatant, and it is 1.28 that relative density is concentrated under reduced pressure at pressure 0.1MPa, 55 DEG C of temperature, Obtain black cloth woods extract.
Embodiment 2
(1) fresh black cloth woods are taken, fruit stone is removed, squeezes the juice, sterilize 20min at 121 DEG C, cools down, is filtrated to get black cloth woods juice；
(2) saccharomycete bacteria suspension is prepared：Saccharomycete is inoculated into broth bouillon, the Ju Ti Pei Fang of broth bouillon is (every liter)：Peptone 10g, beef extract powder 3g, sodium chloride 5g, final pH 7.3 ± 0.1 is in 37 DEG C of temperature, speed of agitator 48h is cultivated under conditions of 50rpm, takes nutrient solution in being centrifuged under 8000rpm, the wet thallus after centrifugation is standby；
(3) sodium alginate-CaCl of saccharomycete2Immobilization：The sodium alginate soln that mass fraction is 5% is prepared, then The saccharomycete wet thallus prepared in 4% step (2) of its volume is added, mixed liquor is configured to, passes through 3mm with peristaltic pump The mixed liquor prepared is added dropwise to the CaCl that mass fraction is 4% by the syringe needle in aperture2In solution, balling-up, and by CaCl2 Solution and bead be stored in -4 DEG C under the conditions of 20h, take out bead, with aseptic water washing, dry for standby；
(4) by the immobilized yeast bead prepared in black cloth woods juice and step (3) according to mass ratio 3:1 mixing, in temperature Spend for 24 DEG C, speed of agitator is the 60h that fermented under conditions of 4rpm；It is subsequently placed in 10 DEG C of refrigerators and preserves 5d, then under normal temperature Place 18h；
(5) step (4) fermentation after fermentation liquid is filtered, obtains clarified broth, 85% alcohol for adding 3 times of its quality is molten Liquid, precipitates 30h, filters to take supernatant, and it is 1.30 that relative density is concentrated under reduced pressure at pressure 0.1MPa, 55 DEG C of temperature, Obtain black cloth woods extract.
Embodiment 3
(1) fresh black cloth woods are taken, fruit stone is removed, squeezes the juice, sterilize 20min at 121 DEG C, cools down, is filtrated to get black cloth woods juice；
(2) saccharomycete bacteria suspension is prepared：Saccharomycete is inoculated into broth bouillon, the Ju Ti Pei Fang of broth bouillon is (every liter)：Peptone 10g, beef extract powder 3g, sodium chloride 5g, final pH 7.3 ± 0.1 is in 37 DEG C of temperature, speed of agitator 48h is cultivated under conditions of 50rpm, takes nutrient solution in being centrifuged under 8000rpm, the wet thallus after centrifugation is standby；
(3) sodium alginate-CaCl of saccharomycete2Immobilization：The sodium alginate soln that mass fraction is 5% is prepared, then The saccharomycete wet thallus prepared in 5% step (2) of its volume is added, mixed liquor is configured to, passes through 4mm with peristaltic pump The mixed liquor prepared is added dropwise to the CaCl that mass fraction is 6% by the syringe needle in aperture2In solution, balling-up, and by CaCl2 Solution and bead be stored in -4 DEG C under the conditions of 24h, take out bead, with aseptic water washing, dry for standby；
(4) by the immobilized yeast bead prepared in black cloth woods juice and step (3) according to mass ratio 5:1 mixing, in temperature Spend for 28 DEG C, speed of agitator is the 96h that fermented under conditions of 1rpm；It is subsequently placed in 10 DEG C of refrigerators and preserves 6d, then under normal temperature Place 24h；
(5) step (4) fermentation after fermentation liquid is filtered, obtains clarified broth, 90% alcohol for adding 4 times of its quality is molten Liquid, precipitates 40h, filters to take supernatant, and it is 1.33 that relative density is concentrated under reduced pressure at pressure 0.1MPa, 55 DEG C of temperature, Obtain black cloth woods extract.
The black cloth woods extract prepared in above-described embodiment has abundant cigarette perfume, lifts cigarette flavor quality, increases cigarette The fragrance property sent out thoroughly, increase flue gas promotes the production of body fluid sense, increases the sweet sense of flue gas, soft fine and smooth flue gas, reduction excitant, improvement throat is comfortable Property, miscellaneous gas is covered, improves the effect such as pleasant impression.
Contrast experiment, the black cloth that the black cloth woods extract that applicant prepares the present invention is prepared with existing traditional extraction process Woods extract, the black cloth woods extract prepared that fermented using suspended yeast bacterium are added in pipe tobacco according to normal perfuming mode, are made Into cigarette, and smoked panel test, its effect of smokeing panel test is as shown in table 1：
The sensory evaluation effect of black cloth woods extract prepared by the different process of table 1 in cigarette compares
It can be seen that to come from above-mentioned contrast experiment, the black cloth woods extract for preparing is added in cigarette effect more in the present invention It is good, and found according to specific experiment analysis, compared with suspended yeast bacterium is fermented, ester in black cloth woods extract prepared by the present invention Class Aromatic Matter Contents add 13.58%~17.29%, and benzyl carbinol content adds 9.27%~13.54%, cigarette effect In evaluation, fragrance more enriches, sent out thoroughly, and the fragrant and sweet rhythm of fruit is more obvious.
The detailed description of specific embodiments of the present invention is the foregoing is only, the present invention is not limited with this, it is all at this Any modification, equivalent and improvement for being made in the mentality of designing of invention etc., should be included in protection scope of the present invention it It is interior.

Claims (4)

1. a kind of method that immobilized yeast prepares cigarette black cloth woods extract, it is characterised in that comprise the following steps：
(1) fresh black cloth woods are taken, fruit stone is removed, squeezes the juice, sterilize 20min at 121 DEG C, cools down, is filtrated to get black cloth woods juice；
(2) saccharomycete bacteria suspension is prepared：Saccharomycete is inoculated into broth bouillon, is 50rpm in 37 DEG C of temperature, speed of agitator Under conditions of cultivate 48h, take nutrient solution in being centrifuged under 8000rpm, the wet thallus after centrifugation is standby；
(3) sodium alginate-CaCl of saccharomycete2Immobilization：The sodium alginate soln that mass fraction is 3%~6% is prepared, then The saccharomycete wet thallus prepared in 3%~5% step (2) of its volume is added, mixed liquor is configured to, is passed through with peristaltic pump The mixed liquor prepared is added dropwise to the CaCl that mass fraction is 3%~6% by the syringe needle for crossing 1~5mm apertures2In solution, Balling-up, and by CaCl2Solution and bead be stored in -4 DEG C under the conditions of 12~24h, take out bead, with aseptic water washing, dry standby With；
(4) by the immobilized yeast bead prepared in black cloth woods juice and step (3) according to mass ratio 2:1~5:1 mixing, in temperature Spend for 20~28 DEG C, speed of agitator is 48~96h of fermentation under conditions of 1~5rpm；Be subsequently placed in 10 DEG C of refrigerators preserve 4~ 7d, then in 12~24h of placement under normal temperature；
(5) step (4) fermentation after fermentation liquid is filtered, obtains clarified broth, add 80%~95% wine of 2~4 times of its quality Smart solution, precipitates 24~48h, and it is 1.25~1.35 to filter to take supernatant and be concentrated under reduced pressure into relative density, obtains black cloth woods extract.
2. the method that immobilized yeast according to claim 1 prepares cigarette black cloth woods extract, it is characterised in that：Institute The saccharomycete stated in step (2) is saccharomyces cerevisiae (Saccharomyces cerevisiae) HHL-1, and deposit number is CCTCC NO:M 209272。
3. the method that immobilized yeast according to claim 1 prepares cigarette black cloth woods extract, it is characterised in that：Institute The Ju Ti Pei Fang for stating broth bouillon in step (2) is (every liter)：Peptone 10g, beef extract powder 3g, sodium chloride 5g, finally pH7.3±0.1。
4. the method that immobilized yeast according to claim 1 prepares cigarette black cloth woods extract, it is characterised in that：Institute State the condition being concentrated under reduced pressure in step (5)：Concentrate pressure 0.1MPa, 55 DEG C of thickening temperature.
CN201610895931.1A 2016-10-13 2016-10-13 A kind of method that immobilized yeast prepares cigarette black cloth woods extract Pending CN106957863A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109517874A (en) * 2018-11-27 2019-03-26 湖北中烟工业有限责任公司 A kind of antibacterial agent screening technique extending cigarette berries extract shelf life

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CN101671699A (en) * 2009-10-20 2010-03-17 东华大学 Method for preparing ethanol by fermenting tobacco leftovers by mixing composite carrier and immobilized yeast
CN102210483A (en) * 2010-04-06 2011-10-12 湖北中烟工业有限责任公司 Grape medlar spices for fermentation cigarettes and preparation method thereof
CN102851117A (en) * 2012-09-11 2013-01-02 鹰潭华宝香精香料有限公司 Method for preparing flavor by using apple juice and plum extract
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Publication number Priority date Publication date Assignee Title
CN101671699A (en) * 2009-10-20 2010-03-17 东华大学 Method for preparing ethanol by fermenting tobacco leftovers by mixing composite carrier and immobilized yeast
CN102210483A (en) * 2010-04-06 2011-10-12 湖北中烟工业有限责任公司 Grape medlar spices for fermentation cigarettes and preparation method thereof
CN102851117A (en) * 2012-09-11 2013-01-02 鹰潭华宝香精香料有限公司 Method for preparing flavor by using apple juice and plum extract
CN103146485A (en) * 2013-03-04 2013-06-12 湖北中烟工业有限责任公司 Method for preparing fruit composite extract for fermented cigarette

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109517874A (en) * 2018-11-27 2019-03-26 湖北中烟工业有限责任公司 A kind of antibacterial agent screening technique extending cigarette berries extract shelf life

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Application publication date: 20170718