CN103146485A - Method for preparing fruit composite extract for fermented cigarette - Google Patents
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Abstract
The invention provides a method for preparing a fruit composite extract for fermented cigarettes, belongs to the technical field of tobacco and relates to a preparation method of a tobacco flavor. The method comprises the following steps of: 1) preparation of a culture medium, 2) inoculation, 3) fermentation and 4) separation to obtain the fruit composite extract for cigarettes. According to the invention, raisin and black brin rich in sugar are used as raw materials, and the fruit composite extract with fruit sweet aroma for fermented cigarette is produced through microorganism fermentation technology and has effects of enriching the cigarette aroma, increasing sweet aroma, moderating smoke, increasing return sweet feeling in mouth cavity, improving mouth feel and reducing residue. The method provided by the invention produces the tobacco flavor by the microorganism fermentation technology, and can obtain a way of economically and efficiently producing the natural tobacco flavor in an oriented manner.
Description
Technical field
The present invention relates to the preparation method of tobacco aromatics using, specifically refer to a kind of fermentation type tobacco compound medicinal extract preparation method of fruit.
Background technology
In tobacco product, the fragrance of tobacco and suction flavor are vital, interpolation spices is the key link in cigarette process, is also to improve product style and improve one of effective means of cigarette flavor quality, can some fragrance is provided and inhale the satisfied cigarette products of distinguishing the flavor of to the human consumer.The production of flavouring essence for tobacco, spices is mainly synthetic and extracts from natural animal-plant in the world at present.The flavouring essence for tobacco of synthetic, spices are because people have arguement to its safety issue always, thereby its application is restricted.The trend that goes back to nature along with the mankind, people increase day by day to the flavouring essence for tobacco of safety, the demand of spices, the cigarette that extracts from animals and plants can satisfy the demand of this security with natural essence, spices, but cigarette is subjected to the restriction of raw material sources with natural animal-plant spices.As produce 1kg Flower of Arabian Jasmine essential oil need 5 * 106kg Flower of Arabian Jasmine, thereby the development and utilization of natural perfume material is restricted.Biotechnology is the high-tech technology that develops rapidly in recent years, biotechnological means is the forming process of simulation natural animal-plant spices, the essence, the spices that adopt biotechnology to produce can be equal to natural essence, spices, and it can also make up the insufficient raw material of natural plant pefume.By the fragrance material of biotechnology development and production high value, for a brand-new direction has been opened up in the production of natural perfume.
Utilizing biotechnology to produce natural essence spices mainly realizes by following means:
1, utilize gene engineering, improve the gene character of spice berry, turn out the vegetable cell strain of high yield spices;
2, utilize vegetable cell large scale culturing technology, cultivate in a large number spice berry, thereby obtain the fragrance material of high value;
3, utilize enzyme engineering technology, the fragrance material of synthetic high value take enzyme as the catalyzer bio-transformation;
4, utilize microbial fermentation technology, by screening the microorganism that can produce in a large number and accumulate fragrance material, through the fragrance material of microbial fermentation production high value.That microbial fermentation technology has is with a long history, cost is lower, simple operation and other advantages, and therefore, it is the Main Means that present biotechnology prepares natural essence spices.
Have in cigarette by the prepared raisin of traditional reflux extraction, the compound medicinal extract of black cloth woods fruit and enrich cigarette perfume (or spice), increase the effect that sweet sense is returned in the oral cavity, increase pungency, increase is residual, flue gas is coarse counter productive but also have simultaneously.Raisin extract, plum medicinal extract are perfume monomers common in tobacco, be applied in cigarette, and will prepare the fermentation type tobacco compound medicinal extract of fruit by microbial fermentation technology after fragrant prince wife king's raisin, black currant raisin, black cloth woods three mixed extraction, be applied to have no report in cigarette.
Summary of the invention
The object of the invention is to overcome the deficiencies in the prior art, a kind of fermentation type tobacco compound medicinal extract preparation method of fruit is provided, utilize the Angel TH aroma-producing active dry yeast to produce the fermentation type tobacco compound medicinal extract of fruit, develop the Novel tobacco flavor that traditional production of flavor method can not be produced, increased the category of tobacco aromatics using.
For solving the problems of the technologies described above, the invention provides a kind of fermentation type tobacco compound medicinal extract preparation method of fruit, comprise the following steps:
1) substratum preparation
A. raw material pre-treatment: with the stoning of black cloth woods, be broken into the macrobead shape; By weight 25 ~ 50 raisin and 50 ~ 75 parts of black cloth woodss are mixed, mixed raw material and water are 1 ︰ 5 ~ 15 refluxing extraction 2 ~ 5h according to mass ratio, and then by membrane filtration, filtrate is concentrated, and obtaining relative density is 1.03 ~ 1.12 composite extracts;
B. substratum preparation: add accounting for the glucose of extract quality 0.1% ~ 5% and 0.1% ~ 0.9% sodium-chlor in the composite extract for preparing, sterilization 15 ~ 30min makes substratum;
2) inoculation
Under aseptic condition, aroma-producing active dry yeast is inoculated in substratum, inoculative proportion is 0.01% ~ 2.0% of substratum quality;
3) fermentation
Be 2.5 ~ 7.0 in the pH value, mixing speed is 100 ~ 200rpm, and air flow is 1 ~ 4VVM, and leavening temperature is 20 ~ 35 ℃ of bottom fermentation 2-9d, obtains fermented liquid;
4) separate
Fermented liquid by the membrane filtration removal of impurities, is obtained clear liquor, clear liquor is concentrated into 1/5 ~ 1/2 of original volume, after adding in the concentrated solution 95% ethanol alcohol precipitation of 1 ~ 3 times of concentrated solution quality to process, concentratedly after filtering to get compound medicinal extract, its relative density is 1.20 ~ 1.35.
Further, in described step 1), raisin are comprised of 25 ~ 65% black currant raisin and 35 ~ 75% fragrant prince wife king's raisin by weight percentage.
Again further, in described preparation process, the aperture of filter membrane is less than or equal to 1.5um.
Inventive principle:
Fragrant prince wife king's raisin are without seed, and grain is large, and mouthfeel is fragrant and sweet, and doing due to this grape variety is that on qianlong years, tribute to fragrant prince wife and emperor and high official and noble lord's tribute, hence obtains one's name.This raisin are evenly large, and length is at 2.5 ~ 3.5mm, shape growth strip, and entrance is sweet, leaves one light lingering fragrance after eating in mouth, can fine grade, and parch the sticking group of not making moist.
Fragrant prince wife king's raisin form for the seedless kyoto grape screening of the large grain of the modified rear plantation in recent years smile that dries in the air, and the green middle band of color and luster is white, and grain is seedless greatly, fragrant and sweet good to eat, and the fragrance of approximate Rose is arranged.
Contain the various trace elements of abundant natural fructose, protein, VITAMIN, Mierocrystalline cellulose and needed by human body in fragrant prince wife king's raisin, the nutritions such as iron, potassium, calcium, phosphorus, magnesium have " pearl " laudatory title.
The black currant raisin, black currant, formal name used at school black-current, Xinjiang Kazakh are the OK a karaoke club Hart.So black currant raisin and a kind of very similar called after black currant of fruit raisin that are black currant.This grape growth is at Altay mountain range northern area, and due to the weather effect of catching a cold, growth time is long, is innately the appearance of black, is the rare raisin in the world.Black currant is thick without seed meat, and is aromatic pleasant, and natural aromatic vinous is arranged, and chews strength pure.Wherein contain very abundant carbohydrate, Vitamin P complex, vitamins C, phosphorus, magnesium, potassium, calcium, anthocyanidin, aldehydes matter.
The black cloth woods is exactly black plum in fact, belongs to a kind of of plum.The fruit of black cloth woods contains abundant sugar, VITAMIN, and tartaric acid, the nutritive ingredients such as amino acid, pulp contains total reducing sugar 10.4%, total acid 0.8%, sugar-acid ratio 11.3: 1, soluble solid 11.5%.The black cloth woods is listed in first of " five fruit ", and medical circle thinks, the black cloth woods has to be separated strongly fragrant poison, invigorate blood circulation and promote the production of body fluid, quench one's thirst effects such as drawing drink, cough-relieving apophlegmatic diuresis, ease constipation.
Aroma-producing active dry yeast refers to can produce the yeast of aromatic odour material in growth, reproductive process, it can decompose the part polysaccharide material in black currant raisin, fragrant prince wife king's raisin, black cloth woods mixed extract, eliminate extract to the negative impact of pungency and the mouthfeel of cigarette, and produce micromolecular aromatoising substance, thereby enrich cigarette flavor and soft flue gas.The present invention is take the black currant raisin that are rich in sugar, fragrant prince wife king's raisin, black cloth woods as raw material, utilize the compoiste fermented extract of aroma-producing active dry yeast, suitably reduce macromolecular polysaccharide content in composite extract, be translated into the alcohols aroma substance that has the fresh and sweet note of increase and soft flue gas effect in cigarette, and obtain the quality characteristic cigarette compound medicinal extract of fruit preferably.
Beneficial effect of the present invention is:
1, the present invention is to be rich in sugared fragrant prince wife king's raisin, black currant raisin, black cloth woods as raw material, have the fragrant and sweet fermentation type tobacco of fruit with the compound medicinal extract of fruit by microbial fermentation technology production, it has in cigarette and enriches cigarette perfume (or spice), increases fragrant and sweet, soft flue gas, increases the oral cavity and return sweet sense, improve mouthfeel, reduce residual effect.
2, using microbe fermentation technique of the present invention is produced tobacco aromatics using, can obtain the approach of a kind of economy, the natural tobacco aromatics using of efficient and orientable production, and develop the tobacco aromatics using that traditional production of flavor method can not be produced.
3, the prepared fermentation type tobacco of the present invention has the fresh and sweet fruital note of black currant raisin, fragrant prince wife king's raisin, black cloth woods with the compound medicinal extract spices of fruit, and with the sweet and pure fragrance of pleasant; Add in cigarette, it can increase fresh and sweet note and soft flue gas, reduce the pungency of cigarette and improve mouthfeel, alleviate residual.
Embodiment
In order to explain better the present invention, further illustrate main contents of the present invention below in conjunction with specific embodiment, but content of the present invention not only is confined to following examples.
Embodiment 1
A kind of fermentation type tobacco compound medicinal extract preparation method of fruit comprises the following steps:
1) substratum preparation
A. raw material pre-treatment: with the stoning of black cloth woods, be broken into the macrobead shape; By weight than 25 raisin and 75 parts of black cloth woodss are mixed, mixed raw material and water are 1 ︰ 15 refluxing extraction 5h according to mass ratio, then by the 0.8mm membrane filtration, filtrate is concentrated, obtaining relative density is 1.12 composite extracts, wherein, raisin are comprised of 25% black currant raisin and 75% fragrant prince wife king's raisin by weight percentage;
B. substratum preparation: add accounting for the glucose of extract quality 5% and 0.1% sodium-chlor in the composite extract for preparing, sterilization 30min makes substratum;
2) inoculation
Under aseptic condition, aroma-producing active dry yeast is inoculated in substratum, inoculative proportion is 0.01% of substratum quality;
3) fermentation
Be 2.5 in the pH value, mixing speed is 200rpm, and air flow is 4VVM, and leavening temperature is 35 ℃ of bottom fermentation 2d, obtains fermented liquid;
4) separate
Fermented liquid by the removal of impurities of 0.8mm membrane filtration, is obtained clear liquor, clear liquor is concentrated into 1/2 of original volume, after adding in the concentrated solution 95% ethanol alcohol precipitation of 1 times of concentrated solution quality to process, concentratedly after filtering to get compound medicinal extract, its relative density is 1.35.
Above-mentioned aroma-producing active dry yeast is purchased from Hubei Angel Yeast Co.,Ltd.
Embodiment 2
A kind of fermentation type tobacco compound medicinal extract preparation method of fruit comprises the following steps:
1) substratum preparation
A. raw material pre-treatment: with the stoning of black cloth woods, be broken into the macrobead shape; By weight 50 raisin and 50 parts of black cloth woodss are mixed, mixed raw material and water are 1 ︰ 5 refluxing extraction 2h according to mass ratio, then by the 1.5mm membrane filtration, filtrate is concentrated, obtaining relative density is 1.03 composite extracts, wherein, raisin are comprised of 65% black currant raisin and 35% fragrant prince wife king's raisin by weight percentage;
B. substratum preparation: add accounting for the glucose of extract quality 0.1% and 0.1% sodium-chlor in the composite extract for preparing, sterilization 15min makes substratum;
2) inoculation
Under aseptic condition, aroma-producing active dry yeast is inoculated in substratum, inoculative proportion is 0.01% of substratum quality;
3) fermentation
Be 7.0 in the pH value, mixing speed is 100rpm, and air flow is 1VVM, and leavening temperature is 20 ℃ of bottom fermentation 9d, obtains fermented liquid;
4) separate
Fermented liquid by the membrane filtration removal of impurities, is obtained clear liquor, clear liquor is concentrated into 1/5 of original volume, after adding in the concentrated solution 95% ethanol alcohol precipitation of 3 times of concentrated solution quality to process, concentratedly after filtering to get compound medicinal extract, its relative density is 1.20.
Above-mentioned aroma-producing active dry yeast is purchased from Hubei Angel Yeast Co.,Ltd.
Embodiment 3
A kind of fermentation type tobacco compound medicinal extract preparation method of fruit comprises the following steps:
1) substratum preparation
A. raw material pre-treatment: with the stoning of black cloth woods, be broken into the macrobead shape; By weight 40 raisin and 60 parts of black cloth woodss are mixed, mixed raw material and water are 1 ︰ 7 refluxing extraction 4h according to mass ratio, then by the 0.5mm membrane filtration, filtrate is concentrated, obtaining relative density is 1.085 composite extracts, wherein, raisin are comprised of 50% black currant raisin and 50% fragrant prince wife king's raisin by weight percentage;
B. substratum preparation: add accounting for the glucose of extract quality 0.3% and 0.5% sodium-chlor in the composite extract for preparing, sterilization 20min makes substratum;
2) inoculation
Under aseptic condition, aroma-producing active dry yeast is inoculated in substratum, inoculative proportion is 1.0% of substratum quality;
3) fermentation
Be 5.0 in the pH value, mixing speed is 150rpm, and air flow is 2.5VVM, and leavening temperature is 30 ℃ of bottom fermentation 6d, obtains fermented liquid;
4) separate
Fermented liquid by the removal of impurities of 0.5mm membrane filtration, is obtained clear liquor, clear liquor is concentrated into 1/3 of original volume, after adding in the concentrated solution 95% ethanol alcohol precipitation of 2 times of concentrated solution quality to process, concentratedly after filtering to get compound medicinal extract, its relative density is 1.25.
Above-mentioned aroma-producing active dry yeast is purchased from Hubei Angel Yeast Co.,Ltd.
Claims (3)
1. a fermentation type tobacco with the compound medicinal extract preparation method of fruit, comprises the following steps:
1) substratum preparation
A. raw material pre-treatment: with the stoning of black cloth woods, be broken into the macrobead shape; By weight 25 ~ 50 raisin and 50 ~ 75 parts of black cloth woodss are mixed, mixed raw material and water are 1 ︰ 5 ~ 15 refluxing extraction 2 ~ 5h according to mass ratio, and then by membrane filtration, filtrate is concentrated, and obtaining relative density is 1.03 ~ 1.12 composite extracts;
B. substratum preparation: add accounting for the glucose of extract quality 0.1% ~ 5% and 0.1% ~ 0.9% sodium-chlor in the composite extract for preparing, sterilization 15 ~ 30min makes substratum;
2) inoculation
Under aseptic condition, aroma-producing active dry yeast is inoculated in substratum, inoculative proportion is 0.01% ~ 2.0% of substratum quality;
3) fermentation
Be 2.5 ~ 7.0 in the pH value, mixing speed is 100 ~ 200rpm, and air flow is 1 ~ 4VVM, and leavening temperature is 20 ~ 35 ℃ of bottom fermentation 2-9d, obtains fermented liquid;
4) separate
Fermented liquid by the membrane filtration removal of impurities, is obtained clear liquor, clear liquor is concentrated into 1/5 ~ 1/2 of original volume, after adding in the concentrated solution 95% ethanol alcohol precipitation of 1 ~ 3 times of concentrated solution quality to process, concentratedly after filtering to get compound medicinal extract, its relative density is 1.20 ~ 1.35.
2. fermentation type tobacco according to claim 1 is with the compound medicinal extract preparation method of fruit, and it is characterized in that: in described step 1), raisin are comprised of 25 ~ 65% black currant raisin and 35 ~ 75% fragrant prince wife king's raisin by weight percentage.
3. fermentation type tobacco according to claim 1 and 2 is with the compound medicinal extract preparation method of fruit, and it is characterized in that: in described preparation process, the aperture of filter membrane is less than or equal to 1.5um.
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| CN2013100687279A CN103146485A (en) | 2013-03-04 | 2013-03-04 | Method for preparing fruit composite extract for fermented cigarette |
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Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103484249A (en) * | 2013-07-30 | 2014-01-01 | 湖北中烟工业有限责任公司 | Pear-fermented tobacco flavor and preparation method thereof |
| CN103773806A (en) * | 2013-06-28 | 2014-05-07 | 湖北中烟工业有限责任公司 | Method for preparing fermented perfume material by using residues extracted from natural plants |
| CN104862074A (en) * | 2015-04-28 | 2015-08-26 | 湖北中烟工业有限责任公司 | Fermentation cigarette compound extract preparation method |
| CN104974851A (en) * | 2015-07-06 | 2015-10-14 | 湖北中烟工业有限责任公司 | Preparation method for black plum extract for cigarette |
| CN105146740A (en) * | 2015-07-28 | 2015-12-16 | 湖北中烟工业有限责任公司 | Preparation method of raisin extract for cigarette and application of raisin extract in cigarette |
| CN106190556A (en) * | 2016-07-22 | 2016-12-07 | 湖北中烟工业有限责任公司 | A kind of preparation method of cigarette fermented type blackberry extractum flavor components |
| CN106957864A (en) * | 2016-10-13 | 2017-07-18 | 武汉黄鹤楼香精香料有限公司 | A kind of method that immobilized yeast prepares tobacco fig extract |
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| CN107232638A (en) * | 2017-07-05 | 2017-10-10 | 湖北中烟工业有限责任公司 | A kind of upgrading purification process of raisin extract and its application |
| CN107904020A (en) * | 2017-12-12 | 2018-04-13 | 湖北中烟工业有限责任公司 | A kind of preparation method of fermentation type tobacco strawberry flavor |
| CN115363236A (en) * | 2022-08-29 | 2022-11-22 | 湖北中烟工业有限责任公司 | Preparation method of tobacco flavor with sour and sweet flavors and application of tobacco flavor |
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| CN103773806B (en) * | 2013-06-28 | 2016-03-09 | 湖北中烟工业有限责任公司 | Extracted form natural plant residue is utilized to prepare the method for fermentation class perfume material |
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| CN104862074A (en) * | 2015-04-28 | 2015-08-26 | 湖北中烟工业有限责任公司 | Fermentation cigarette compound extract preparation method |
| CN104974851B (en) * | 2015-07-06 | 2018-06-08 | 湖北中烟工业有限责任公司 | A kind of preparation method of black cloth woods cigarette extract |
| CN104974851A (en) * | 2015-07-06 | 2015-10-14 | 湖北中烟工业有限责任公司 | Preparation method for black plum extract for cigarette |
| CN105146740A (en) * | 2015-07-28 | 2015-12-16 | 湖北中烟工业有限责任公司 | Preparation method of raisin extract for cigarette and application of raisin extract in cigarette |
| CN105146740B (en) * | 2015-07-28 | 2016-12-07 | 湖北中烟工业有限责任公司 | The preparation method of a kind of cigarette raisin extract and the application in Medicated cigarette thereof |
| CN106190556A (en) * | 2016-07-22 | 2016-12-07 | 湖北中烟工业有限责任公司 | A kind of preparation method of cigarette fermented type blackberry extractum flavor components |
| CN106957864A (en) * | 2016-10-13 | 2017-07-18 | 武汉黄鹤楼香精香料有限公司 | A kind of method that immobilized yeast prepares tobacco fig extract |
| CN106957863A (en) * | 2016-10-13 | 2017-07-18 | 武汉黄鹤楼香精香料有限公司 | A kind of method that immobilized yeast prepares cigarette black cloth woods extract |
| CN106957869A (en) * | 2016-10-13 | 2017-07-18 | 武汉黄鹤楼香精香料有限公司 | A kind of method that immobilized yeast prepares cigarette Fragaia ananassa Duchesne extract |
| CN106957862A (en) * | 2016-10-13 | 2017-07-18 | 武汉黄鹤楼香精香料有限公司 | A kind of immobilized yeast prepares method of the cigarette with flores aurantii extract |
| CN107232638A (en) * | 2017-07-05 | 2017-10-10 | 湖北中烟工业有限责任公司 | A kind of upgrading purification process of raisin extract and its application |
| CN107232638B (en) * | 2017-07-05 | 2018-07-03 | 湖北中烟工业有限责任公司 | A kind of upgrading purification process of raisin extract and its application |
| CN107904020A (en) * | 2017-12-12 | 2018-04-13 | 湖北中烟工业有限责任公司 | A kind of preparation method of fermentation type tobacco strawberry flavor |
| CN107904020B (en) * | 2017-12-12 | 2021-02-02 | 湖北中烟工业有限责任公司 | Preparation method of fermented strawberry flavor for cigarettes |
| CN115363236A (en) * | 2022-08-29 | 2022-11-22 | 湖北中烟工业有限责任公司 | Preparation method of tobacco flavor with sour and sweet flavors and application of tobacco flavor |
| CN115363236B (en) * | 2022-08-29 | 2023-04-21 | 湖北中烟工业有限责任公司 | Preparation method of tobacco flavor with sour flavor and sweet flavor and application of tobacco flavor |
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