CN106957862A - A kind of immobilized yeast prepares method of the cigarette with flores aurantii extract - Google Patents
A kind of immobilized yeast prepares method of the cigarette with flores aurantii extract Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
- C12P1/02—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using fungi
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- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
- A24B15/18—Treatment of tobacco products or tobacco substitutes
- A24B15/24—Treatment of tobacco products or tobacco substitutes by extraction; Tobacco extracts
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- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
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- A24B3/12—Steaming, curing, or flavouring tobacco
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- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
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- C12N1/185—Saccharomyces isolates
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- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
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- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
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- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
- C12R2001/85—Saccharomyces
- C12R2001/865—Saccharomyces cerevisiae
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Abstract
The present invention provides a kind of immobilized yeast and prepares method of the cigarette with flores aurantii extract.The preparation method is concretely comprised the following steps：(1) take flores aurantii to add water using Microwave Extraction repeatedly, merge extract solution, obtain flores aurantii extract A；(2) wet thallus is obtained after being inoculated into broth bouillon culture centrifugation using saccharomycete, in the mixed solution that wet thallus is added to polyvinyl alcohol and sodium alginate, and with containing CaCl2Boric acid saturated solution and saturation Na2SO4Fixing lactic acid bacteria bead is crosslinked to obtain in solution；(3) by flores aurantii extract A and fixing lactic acid bacteria bead according to mass ratio 5~10:1 mixing after fermentation, then filters to obtain clarified broth, successively after freeze-drying, alcohol precipitation, filtering, concentration, molecular distillation, and it is flores aurantii extract to take light component.Of the invention to have abundant cigarette perfume, fruit is fragrant and sweet and fragrance of a flower rhythm is more obvious, and oral cavity pleasant impression is more comfortable, increases the cigarette flavor property sent out thoroughly.
Description
Technical field
The present invention relates to cigarette additive field, and in particular to prepares cigarette flores aurantii extract using immobilized yeast
Preparation method.
Background technology
Flavoring and casing is the key link in cigarette process, is the determinant and raising cigarette flavor for improving product style
One of effective means of quality.Current essence spice for cigarette is main or passes through the extraction to natural animal-plant, chemical synthesis system
It is standby.Due to by natural plant resource is limited and the harsher limitation of chemical synthesis condition.With the development of biotechnology, in recent years
Come, prepare cigarette flavor using microbe fermentation method, an economy, efficient approach are provided for the production of cigarette flavor,
Develop the new cigarette flavor that some conventional methods can not be produced.
Immobilized microorganism technology, is to be fixed on special microorganism on the carrier of choosing card, makes its highly dense and protect
Bioactivity is held, the biotechnology that can quickly, largely breed under condition of compatibility can make up suspension microorganism fermentation process
The shortcomings of efficiency is low, separation of solid and liquid is difficult, mithridatism is poor.Therefore, in recent years, the research of immobilized microorganism technology is lived very much
Jump, development is very fast, has application to many industrial departments such as food fermentation industry, environmental protection, new energy development and biological biography
Sensor etc., and deep development is achieved, show powerful superiority.It is mainly concerned with the following aspects：In food hair
It is used to produce ethanol, beer, Yoghourt etc. in ferment industry；It is used for environmental monitoring and the processing of industrial wastewater in environmental protection；
It is used to produce hydrogen, methane, biochemical fuel cell, microorganism battery etc. in new energy development；Immobilized microorganism is alternatively arranged as molecule
Recognition component, for making various biology sensors.And it is continuous ripe, perfect with fixation of microbe technology, gradually to height
The direction such as effect low energy consumption, low cost, simplification, safety automation, pollution-free is developed, dirty in energy crisis, shortage of resources, environment
In the case that dye is on the rise, it thus will be promoted more deeply and to be widely applied in terms of wastewater treatment, with more deepening
Remote meaning.Especially in terms of wastewater treatment, have great application prospect.But preparing cigarette using immobilized microorganism technology
Report is but had no with flores aurantii extract.
Flores aurantii is the white petal of bigarabe, and Rutaceae citrus plant originates in South of France, Morocco, Egypt and meaning
The ground such as big profit.Spices flores aurantii essential oil and flores aurantii absolute oil by raw material of flores aurantii are the cigarette additives commonly used in cigarette, in cigarette
In there is the effect of abundant cigarette perfume, the increase fragrance of a flower and fragrant and sweet note.
Saccharomycete is some unicellular fungis, is sac fungus, the common name of basidiomycetes Deng Ji sections unicellular fungi, typically refers to
The fungi of energy fermenting carbohydrate, available for production is brewageed, the bacterium can produce the fragrance thing such as ethyl acetate, benzyl carbinol during the fermentation
Matter, these aroma substances can assign the fragrant and sweet fragrance of cigarette feature, be a kind of high-quality aroma former for producing tobacco aromaticss.
The content of the invention
Method of the cigarette with flores aurantii extract is prepared it is an object of the invention to provide a kind of immobilized yeast, with reference to microorganism
The technologies such as immobilization fermentation, freeze-drying and molecular distillation, develop a kind of Novel tobacco flores aurantii extract.
The technical scheme that the present invention is provided：A kind of immobilized yeast prepares method of the cigarette with flores aurantii extract, its
It is characterised by specifically including following steps：
(1) flores aurantii is taken, the water of 5~10 times of its weight is added, Microwave Extraction 2~3 times at 50~65 DEG C, when extracting every time
Between be 2~3 hours, be cooled to room temperature after having extracted, and be filtrated to get extract solution, merge multiple extract solution, obtain flores aurantii and extract
Thing A；
(2) saccharomycete is inoculated into broth bouillon, cultivated under conditions of 37 DEG C of temperature, speed of agitator are 50rpm
48 hours, take nutrient solution in being centrifuged under 8000rpm, the wet thallus after centrifugation is standby；
(3) polyvinyl alcohol-Immobilization in Sodium Alginate of saccharomycete：It is water-soluble with sodium alginate using polyvinyl alcohol water solution
Liquid prepares the polyvinyl alcohol containing mass percent 6%~10% and the mixing of the sodium alginate of mass percent 1%~3% is molten
The saccharomycete wet thallus prepared in liquid, the step of the 1~3% of total solution volume is then added into mixed solution (2), is configured to
Mixed liquor, CaCl is added dropwise to peristaltic pump by the syringe needle in 1~5mm apertures by the mixed liquor prepared2Quality percentage
Than in the boric acid saturated solution for 1~3%, then bead is taken out and adds saturation Na by crosslinking 1h formation beads2SO4In solution
3-5h is crosslinked, PVA-SA immobilized yeast beads, then using aseptic water washing, dry for standby is obtained；
(4) the flores aurantii extract A that will be prepared in step (1) and the immobilized yeast bead that is prepared in step (3) according to
Mass ratio 5~10:1 mixing, is 15~22 DEG C in temperature, and speed of agitator is 3~5d of fermentation under conditions of 1~5rpm, is then put
7~14d is preserved in 4 DEG C of refrigerators；
(5) step (4) fermentation after fermentation liquid is filtered, obtains clarified broth, then carried out the zymotic fluid after filtering cold
Lyophilized dry, its feed liquid thickness being freeze-dried is 1~2 centimetre, and vacuum is 10Pa, and drying time is 6~12h；
(6) zymotic fluid after being freeze-dried in step (5) is taken, 80~95% alcoholic solutions of 2~4 times of its quality are added,
Precipitation 24~48 hours, it is 0.1MPa to filter to take supernatant in pressure, and temperature is to be concentrated into relative density under conditions of 45 DEG C to be
1.25~1.30；Then propane diols is added in concentrate, flores aurantii extract B is obtained, the addition of the propane diols is gross mass
20%；
(7) the flores aurantii extract B that will be prepared in step (6), in 0.5~5Pa of pressure, feeding temperature is 55 DEG C~60 DEG C,
Charging rate is 5~15ml/min, and scraper plate rotating speed is carries out molecular distillation under conditions of 80~100rpm, and it is flores aurantii to take light component
Extract.
The saccharomycete used in the present invention is saccharomyces cerevisiae (Saccharomyces cerevisiae) HHL-1, in 2009
It is preserved in Wuhan University's China typical culture collection center on November 17, in, deposit number is CCTCC NO:M 209272.
The immobilized microorganism technology that the present invention is used has that fermentation process efficiency high, separation of solid and liquid be easy, mithridatism is strong
The advantages of, the deficiency of traditional biological technique can be made up.
The present invention, using immobilized yeast fermentation flores aurantii extract, obtains a kind of quality excellent using flores aurantii as raw material
Good cigarette flores aurantii extract, the extract has abundant cigarette perfume in cigarette, increases fragrant and sweet and floral notes, increase cigarette fragrant
The gas property sent out thoroughly, oral cavity pleasant impression is relatively comfortable.Compared with existing flores aurantii medicinal extract and flores aurantii extract, the flores aurantii extract is fragrant in cigarette
Gas more enriches, and fragrant and sweet rhythm and the fragrance of a flower rhythm is more obvious, and the property sent out is more preferable thoroughly for cigarette flavor；, should compared with suspended yeast bacterium is fermented
Esters fragrance content of material adds 13.68%~17.29% in flores aurantii extract prepared by method, and benzyl carbinol content is added
23.47%~30.11%, in cigarette effect assessment, fragrance more enriches, and fruit is fragrant and sweet and fragrance of a flower rhythm is more obvious, oral cavity pleasant impression
It is more comfortable.This method instead of the traditional extractive technique of flores aurantii extract, have updated biofermentation preparation technology so that its energy
Enough applicability increases in cigarette, a new way is provided to cigarette with the preparation of flores aurantii extract.
Embodiment
With reference to embodiment to further detailed description of the present invention.
PVA-SA (polyvinyl alcohol-sodium alginate) immobilized yeast bead in following examples of the present invention be according to
Lower method is obtained using polyvinyl alcohol (PVA) and sodium alginate (SA) to being fixed of saccharomycete：
(1) saccharomycete is inoculated into broth bouillon, cultivated under conditions of 37 DEG C of temperature, speed of agitator are 50rpm
48 hours, take nutrient solution in being centrifuged under 8000rpm, the wet thallus after centrifugation is standby；It is conventional that agar culture is supported in the above method
Broth medium, its Ju Ti Pei Fang be (every liter)：Peptone 10g, beef extract powder 3g, sodium chloride 5g, final pH 7.3 ±
0.1；
(2) polyvinyl alcohol-Immobilization in Sodium Alginate of saccharomycete：It is water-soluble with sodium alginate using polyvinyl alcohol water solution
Liquid prepares the polyvinyl alcohol containing mass percent 6%~10% and the mixing of the sodium alginate of mass percent 1%~3% is molten
The saccharomycete wet thallus prepared in liquid, the step of the 1~3% of total solution volume is then added into mixed solution (2), is configured to
Mixed liquor, CaCl is added dropwise to peristaltic pump by the syringe needle in 1~5mm apertures by the mixed liquor prepared2Quality percentage
Than in the boric acid saturated solution for 1~3%, then bead is taken out and is then added to saturation Na by crosslinking 1h formation beads2SO4Solution
Middle crosslinking 3-5h, obtains PVA-SA immobilized yeast beads, then using aseptic water washing, dry for standby；It is described to contain 1-3%
CaCl2Boric acid saturated solution amount and saturation Na2SO4The amount of solution has no particular/special requirement, contains 1-3%CaCl2Boric acid satisfy
Only need to that mixed liquor can be made to produce precipitation with solution, meet the immobilization requirement of lactic acid bacteria, Na2SO4Solution is only needed to
Bead is completely soaked and just may be used；The boric acid saturated solution, saturation Na2SO4Solution is saturated aqueous solution.
The saccharomycete used in the present invention is saccharomyces cerevisiae (Saccharomyces cerevisiae) HHL-1, in 2009
It is preserved in Wuhan University's China typical culture collection center on November 17, in, deposit number is CCTCC NO:M 209272.
A kind of embodiment 1, immobilized yeast prepares method of the cigarette with flores aurantii extract, comprises the following steps：
(1) flores aurantii is taken, the water of 5 times of its quality is added, Microwave Extraction 2 times at 55 DEG C, each extraction time is 2 hours,
Room temperature is cooled to after having extracted, and is filtrated to get extract solution, merges multiple extract solution, obtains flores aurantii extract A, extract twice
Condition is identical；
(2) the PVA-SA immobilized yeast beads for preparing the flores aurantii extract A prepared in step (1) with the above method
According to mass ratio 5:1 mixing, is 15 DEG C in temperature, speed of agitator is the 4d that fermented under conditions of 5rpm, is subsequently placed in 4 DEG C of refrigerators
Preserve 8d；
(3) step (2) fermentation after fermentation liquid is filtered, obtains clarified broth, the zymotic fluid after filtering is freeze-dried,
Feed liquid thickness is 1~2 centimetre, and vacuum is 10Pa, and drying time is 10h；
(4) zymotic fluid after step (3) freeze-drying is taken, 80~95% alcoholic solutions of 3 times of its quality, precipitation 24 are added
Hour, it is 0.1MPa to filter to take supernatant in pressure, and temperature is that to be concentrated into relative density under conditions of 45 DEG C be 1.25, Ran Hou
The propane diols of gross mass 20% is added in concentrate, flores aurantii extract B is obtained；
(5) by the flores aurantii extract B of step (4), in pressure 2Pa, feeding temperature is 55 DEG C, and charging rate is 15ml/
Min, scraper plate rotating speed is carries out molecular distillation under 80rpm Parameter Conditions, and it is flores aurantii extract to take light component.
The flores aurantii extract prepared in embodiment 1 has abundant cigarette perfume in cigarette, and fruit is fragrant and sweet and fragrance of a flower rhythm is more obvious,
Oral cavity pleasant impression is more comfortable, increases the cigarette flavor property sent out thoroughly.
A kind of embodiment 2, immobilized yeast prepares method of the cigarette with flores aurantii extract, comprises the following steps：
(1) flores aurantii is taken, the water of 10 times of its quality is added, Microwave Extraction 3 times at 60 DEG C, each extraction time is 3 small
When, it is cooled to room temperature after having extracted, and be filtrated to get extract solution, merges multiple extract solution, obtain flores aurantii extract A, carries for three times
The condition taken is identical；
(2) the PVA-SA immobilized yeast beads for preparing the flores aurantii extract A prepared in step (1) with the above method
According to mass ratio 10:1 mixing, is 20 DEG C in temperature, speed of agitator is the 5d that fermented under conditions of 3rpm, is subsequently placed in 4 DEG C of refrigerators
Middle preservation 14d；
(3) step (2) fermentation after fermentation liquid is filtered, obtains clarified broth, zymotic fluid is freeze-dried, feed liquid thickness
For 1~2 centimetre, vacuum is 10Pa, and drying time is 12h；
(4) zymotic fluid after step (3) freeze-drying is taken, 80~95% alcoholic solutions of 2 times of its quality, precipitation 24 are added
Hour, it is 0.1MPa to filter to take supernatant in pressure, and temperature is that to be concentrated into relative density under conditions of 45 DEG C be 1.30, Ran Hou
The propane diols of gross mass 20% is added in concentrate, flores aurantii extract B is obtained；
(5) by the flores aurantii extract B of step (4), in 4Pa, feeding temperature is 60 DEG C, and charging rate is 10ml/min, is scraped
Plate rotating speed is 100rpm, carries out molecular distillation, and it is flores aurantii extract to take light component.
The flores aurantii extract prepared in embodiment 2 has abundant cigarette perfume, and fruit is fragrant and sweet and fragrance of a flower rhythm is more obvious, oral cavity pleasant impression
It is more comfortable, increase the cigarette flavor property sent out thoroughly.
A kind of embodiment 3, immobilized yeast prepares method of the cigarette with flores aurantii extract, comprises the following steps：
(1) flores aurantii is taken, the water of 8 times of its quality is added, Microwave Extraction 2 times at 60 DEG C, each extraction time is 3 hours,
Room temperature is cooled to after having extracted, and is filtrated to get extract solution, merges multiple extract solution, obtains flores aurantii extract A, extract twice
Condition is identical；
(2) the PVA-SA immobilized yeast beads for preparing the flores aurantii extract A prepared in step (1) with the above method
According to mass ratio 7:1 mixing, is 18 DEG C in temperature, speed of agitator is the 6d that fermented under conditions of 5rpm, is subsequently placed in 4 DEG C of refrigerators
12d；
(3) step (2) fermentation after fermentation liquid is filtered, obtains clarified broth, and the zymotic fluid after filtering is freezed
Dry, feed liquid thickness is 1~2 centimetre, vacuum is 10Pa, drying time is 6~12h；
(4) zymotic fluid after being freeze-dried in step (3) is taken, 80~95% alcoholic solutions of 4 times of its quality are added, precipitated
48 hours, it was 0.1MPa to filter to take supernatant in pressure, and temperature is that to be concentrated into relative density under conditions of 45 DEG C be 1.30, then
The propane diols of gross mass 20% is added in concentrate, flores aurantii extract B is obtained；
(5) by the flores aurantii extract B of step (4), in 3Pa, feeding temperature is 57 DEG C, and charging rate is 5ml/min, scraper plate
Rotating speed is 80rpm, carries out molecular distillation, and it is flores aurantii extract to take light component.
The flores aurantii extract prepared in embodiment 3 has abundant cigarette perfume, and fruit is fragrant and sweet and fragrance of a flower rhythm is more obvious, oral cavity pleasant impression
It is more comfortable, increase the cigarette flavor property sent out thoroughly.
Contrast experiment, applicant carries the flores aurantii that flores aurantii extract prepared by the present invention is prepared with existing traditional extraction process
Take thing, the flores aurantii extract prepared that fermented using suspended yeast bacterium to be added to according to normal perfuming mode in pipe tobacco, cigarette be made,
And smoked panel test, its effect of smokeing panel test is as follows：
The sensory evaluation effect that flores aurantii prepared by three kinds of different process is extracted in cigarette compares
It can be seen that to come from above-mentioned contrast experiment, the flores aurantii extract for preparing is added in cigarette effect more in the present invention
It is good, and found according to specific experiment analysis, compared with suspended yeast bacterium is fermented, esters in flores aurantii extract prepared by this method
Aromatic Matter Contents add 13.68%~17.29%, and benzyl carbinol content adds 23.47%~30.11%, cigarette effect
In evaluation, fragrance more enriches, and fruit is fragrant and sweet and fragrance of a flower rhythm is more obvious, and oral cavity pleasant impression is more comfortable.
Claims (2)
1. a kind of immobilized yeast prepares method of the cigarette with flores aurantii extract, it is characterised in that specifically include following steps：
(1) flores aurantii is taken, the water of 5~10 times of its weight is added, Microwave Extraction 2~3 times at 50~65 DEG C, each extraction time is
2~3 hours, room temperature is cooled to after having extracted, and is filtrated to get extract solution, merges multiple extract solution, obtains flores aurantii extract A；
(2) saccharomycete bacteria suspension is prepared：Saccharomycete is inoculated into broth bouillon, is 50rpm in 37 DEG C of temperature, speed of agitator
Under conditions of cultivate 48 hours, take nutrient solution in being centrifuged under 8000rpm, the wet thallus after centrifugation is standby；
(3) polyvinyl alcohol-Immobilization in Sodium Alginate of saccharomycete：Matched somebody with somebody using polyvinyl alcohol water solution with sodium alginate aqueous solution
The mixed solution of the polyvinyl alcohol containing mass percent 6%~10% and the sodium alginate of mass percent 1%~3% is made, so
The saccharomycete wet thallus prepared in the step of adding the 1~3% of total solution volume in backward mixed solution (2), is configured to mixing
Liquid, CaCl is added dropwise to peristaltic pump by the syringe needle in 1~5mm apertures by the mixed liquor prepared2Mass percent be 1
In~3% boric acid saturated solution, then bead is taken out and adds saturation Na by crosslinking 1h formation beads2SO4It is crosslinked in solution
3-5h, obtains PVA-SA immobilized yeast beads, then using aseptic water washing, dry for standby；
(4) by the middle immobilized yeast bead prepared of the flores aurantii extract A prepared in step (1) and step (3) according to quality
Than 5~10:1 mixing, is 15~22 DEG C in temperature, and speed of agitator is 3~5d of fermentation under conditions of 1~5rpm, is subsequently placed in 4 DEG C
7~14d is preserved in refrigerator；
(5) step (4) fermentation after fermentation liquid is filtered, obtains clarified broth, it is dry that the zymotic fluid after filtering then is carried out into freezing
Dry, its feed liquid thickness being freeze-dried is 1~2 centimetre, and vacuum is 10Pa, and drying time is 6~12h；
(6) zymotic fluid after being freeze-dried in step (5) is taken, 80~95% alcoholic solutions of 2~4 times of its quality are added, precipitated
24~48 hours, it was 0.1MPa to filter to take supernatant in pressure, and temperature is that to be concentrated into relative density under conditions of 45 DEG C be 1.25
~1.30；Then propane diols is added in concentrate, flores aurantii extract B is obtained, the addition of the propane diols is gross mass
20%；
(7) the flores aurantii extract B that will be prepared in step (6), in 0.5~5Pa of pressure, feeding temperature is 55 DEG C~60 DEG C, charging
Speed is 5~15ml/min, and scraper plate rotating speed is carries out molecular distillation under conditions of 80~100rpm, and it is that flores aurantii is extracted to take light component
Thing.
2. a kind of immobilized yeast according to claim 1 prepares method of the cigarette with flores aurantii extract, it is characterised in that
Saccharomycete in the step (2) is saccharomyces cerevisiae (Saccharomyces cerevisiae) HHL-1, and deposit number is
CCTCC NO:M 209272。
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115011410A (en) * | 2022-06-08 | 2022-09-06 | 吉林烟草工业有限责任公司 | Schisandra chinensis branch spice, preparation method thereof and cigarette |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102210483A (en) * | 2010-04-06 | 2011-10-12 | 湖北中烟工业有限责任公司 | Grape medlar spices for fermentation cigarettes and preparation method thereof |
| CN102406231A (en) * | 2010-09-21 | 2012-04-11 | 湖北中烟工业有限责任公司 | Fermented rose extract for cigarettes and preparation method thereof |
| CN102559796A (en) * | 2012-03-07 | 2012-07-11 | 南京林业大学 | Method for preparing gardenia blue pigment by utilizing immobilized yeast cells |
| CN103146485A (en) * | 2013-03-04 | 2013-06-12 | 湖北中烟工业有限责任公司 | Method for preparing fruit composite extract for fermented cigarette |
-
2016
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102210483A (en) * | 2010-04-06 | 2011-10-12 | 湖北中烟工业有限责任公司 | Grape medlar spices for fermentation cigarettes and preparation method thereof |
| CN102406231A (en) * | 2010-09-21 | 2012-04-11 | 湖北中烟工业有限责任公司 | Fermented rose extract for cigarettes and preparation method thereof |
| CN102559796A (en) * | 2012-03-07 | 2012-07-11 | 南京林业大学 | Method for preparing gardenia blue pigment by utilizing immobilized yeast cells |
| CN103146485A (en) * | 2013-03-04 | 2013-06-12 | 湖北中烟工业有限责任公司 | Method for preparing fruit composite extract for fermented cigarette |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115011410A (en) * | 2022-06-08 | 2022-09-06 | 吉林烟草工业有限责任公司 | Schisandra chinensis branch spice, preparation method thereof and cigarette |
| CN115011410B (en) * | 2022-06-08 | 2023-09-19 | 吉林烟草工业有限责任公司 | Schisandra chinensis branch spice, preparation method thereof and cigarette |
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Application publication date: 20170718 |