CN106957839A - A kind of method that immobilized yeast prepares cigarette Flos Rosae Rugosae extract - Google Patents

A kind of method that immobilized yeast prepares cigarette Flos Rosae Rugosae extract Download PDF

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CN106957839A
CN106957839A CN201610899648.6A CN201610899648A CN106957839A CN 106957839 A CN106957839 A CN 106957839A CN 201610899648 A CN201610899648 A CN 201610899648A CN 106957839 A CN106957839 A CN 106957839A
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flos rosae
rosae rugosae
solution
rugosae extract
extract
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庞登红
尹团章
喻世涛
易建云
潘妮
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Wuhan Huanghelou Flavoring and Essence Co Ltd
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Wuhan Huanghelou Flavoring and Essence Co Ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/10—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a carbohydrate
    • A—HUMAN NECESSITIES
    • A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
    • A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
    • A24B3/00—Preparing tobacco in the factory
    • A24B3/12—Steaming, curing, or flavouring tobacco
    • C—CHEMISTRY; METALLURGY
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    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/18—Baker's yeast; Brewer's yeast
    • C12N1/185—Saccharomyces isolates
    • C—CHEMISTRY; METALLURGY
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    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/04—Enzymes or microbial cells immobilised on or in an organic carrier entrapped within the carrier, e.g. gel or hollow fibres
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
    • C12P1/02—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/85—Saccharomyces
    • C12R2001/865—Saccharomyces cerevisiae

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Abstract

The present invention provides a kind of method that immobilized yeast prepares cigarette Flos Rosae Rugosae extract.The preparation method is concretely comprised the following steps：(1) take rose to add water using Microwave Extraction repeatedly, merge extract solution, obtain Flos Rosae Rugosae extract A；(2) wet thallus is obtained after being inoculated into broth bouillon culture centrifugation using saccharomycete, in the mixed solution that wet thallus is added to polyvinyl alcohol and sodium alginate, and with containing CaCl2Boric acid saturated solution and saturation Na2SO4Fixing lactic acid bacteria bead is crosslinked to obtain in solution；(3) by Flos Rosae Rugosae extract A and fixing lactic acid bacteria bead mixing after fermentation, clarified broth is filtered to obtain, successively after freeze-drying, alcohol precipitation, filtering, concentration, molecular distillation, it is Flos Rosae Rugosae extract to take light component.The present invention, which is added in cigarette, has abundant cigarette perfume, and fresh and sweet note and the fragrance of a flower rhythm is more obvious, and the property sent out is more preferable thoroughly for cigarette perfume.

Description

A kind of method that immobilized yeast prepares cigarette Flos Rosae Rugosae extract
Technical field
The present invention relates to cigarette additive field, and in particular to prepares cigarette Flos Rosae Rugosae extract using immobilized yeast Preparation method.
Background technology
Flavoring and casing is the key link in cigarette process, is the determinant and raising cigarette flavor for improving product style One of effective means of quality.Current essence spice for cigarette is main or passes through the extraction to natural animal-plant, chemical synthesis system It is standby.Due to by natural plant resource is limited and the harsher limitation of chemical synthesis condition.With the development of biotechnology, in recent years Come, prepare cigarette flavor using microbe fermentation method, an economy, efficient approach are provided for the production of cigarette flavor, Develop the new cigarette flavor that some conventional methods can not be produced.
Immobilized microorganism technology, is to be fixed on special microorganism on the carrier of choosing card, makes its highly dense and protect Bioactivity is held, the biotechnology that can quickly, largely breed under condition of compatibility can make up suspension microorganism fermentation process The shortcomings of efficiency is low, separation of solid and liquid is difficult, mithridatism is poor.Therefore, in recent years, the research of immobilized microorganism technology is lived very much Jump, development is very fast, has application to many industrial departments such as food fermentation industry, environmental protection, new energy development and biological biography Sensor etc., and deep development is achieved, show powerful superiority.It is mainly concerned with the following aspects：In food hair It is used to produce ethanol, beer, Yoghourt etc. in ferment industry；It is used for environmental monitoring and the processing of industrial wastewater in environmental protection； It is used to produce hydrogen, methane, biochemical fuel cell, microorganism battery etc. in new energy development；Immobilized microorganism is alternatively arranged as molecule Recognition component, for making various biology sensors.And it is continuous ripe, perfect with fixation of microbe technology, gradually to height The direction such as effect low energy consumption, low cost, simplification, safety automation, pollution-free is developed, dirty in energy crisis, shortage of resources, environment In the case that dye is on the rise, it thus will be promoted more deeply and to be widely applied in terms of wastewater treatment, with more deepening Remote meaning.Especially in terms of wastewater treatment, have great application prospect.But preparing cigarette using immobilized microorganism technology Report is but had no with Flos Rosae Rugosae extract.
Rose original producton location China, belongs to Rosales, rose family machaka.When rose is as crops, its flower is mainly used In food and refinement essence attar of rose, attar of rose is applied to the industry such as cosmetics, food, fine chemistry industry.Using rose as raw material Spices rose extract, rose medicinal extract, Rosa Damascana and rose absolute are the cigarette additives commonly used in cigarette, are had in cigarette There is abundant cigarette perfume, increase fresh and sweet and floral notes, thoroughly the effect such as hair fragrance.
Saccharomycete is some unicellular fungis, is sac fungus, the common name of basidiomycetes Deng Ji sections unicellular fungi, typically refers to The fungi of energy fermenting carbohydrate, available for production is brewageed, the bacterium can produce the fragrance thing such as ethyl acetate, benzyl carbinol during the fermentation Matter, these aroma substances can assign the fragrant and sweet fragrance of cigarette feature, be a kind of high-quality aroma former for producing tobacco aromaticss.
The content of the invention
It is an object of the invention to provide a kind of method that immobilized yeast prepares cigarette Flos Rosae Rugosae extract, with reference to micro- life The technologies such as thing immobilization fermentation, freeze-drying and molecular distillation, develop a kind of Novel tobacco Flos Rosae Rugosae extract.
The technical scheme that the present invention is provided：The method that a kind of immobilized yeast prepares cigarette Flos Rosae Rugosae extract, It is characterized in that specifically including following steps：
(1) rose is taken, the water of 3~8 times of its weight is added, Microwave Extraction 2~3 times at 50~65 DEG C are extracted every time Time is 2~3 hours, and room temperature is cooled to after having extracted, and is filtrated to get extract solution, merges multiple extract solution, obtains rose Extract A；
(2) saccharomycete is inoculated into broth bouillon, cultivated under conditions of 37 DEG C of temperature, speed of agitator are 50rpm 48 hours, take nutrient solution in being centrifuged under 8000rpm, the wet thallus after centrifugation is standby；
(3) polyvinyl alcohol-Immobilization in Sodium Alginate of saccharomycete：It is water-soluble with sodium alginate using polyvinyl alcohol water solution Liquid prepares the polyvinyl alcohol containing mass percent 6%~10% and the mixing of the sodium alginate of mass percent 1%~3% is molten The saccharomycete wet thallus prepared in liquid, the step of the 1~3% of total solution volume is then added into mixed solution (2), is configured to Mixed liquor, CaCl is added dropwise to peristaltic pump by the syringe needle in 1~5mm apertures by the mixed liquor prepared2Quality percentage Than in the boric acid saturated solution for 1~3%, 1h being crosslinked, then in saturation Na2SO43-5h is crosslinked in solution, PVA-SA is obtained and fixes Change saccharomycete bead, then using aseptic water washing, dry for standby；
(4) the Flos Rosae Rugosae extract A prepared in step (1) and the immobilized yeast bead of preparation in step (3) are pressed According to mass ratio 5~10:1 mixing, is 20~25 DEG C in temperature, and speed of agitator is 2~4d of fermentation under conditions of 1~5rpm, then It is placed in 7~14d of preservation in 4 DEG C of refrigerators；
(5) step (4) fermentation after fermentation liquid is filtered, obtains clarified broth, zymotic fluid is freeze-dried, feed liquid thickness For 1~2 centimetre, vacuum is 10Pa, and drying time is 6~12h；
(6) zymotic fluid after step (5) freeze-drying is taken, the alcohol of mass concentration 80~95% of 2~4 times of its quality is added Solution, is precipitated 24~48 hours, and it is 0.1MPa to filter to take supernatant in pressure, and temperature is relatively close to be concentrated under conditions of 45 DEG C Spend for 1.25~1.35, propane diols is then added in concentrate, Flos Rosae Rugosae extract B is obtained, the addition of the propane diols is The 20% of gross mass；
(7) by the Flos Rosae Rugosae extract B of step (6), in 1~10Pa of pressure, feeding temperature is 55 DEG C~70 DEG C, charging speed Spend for 20~50ml/min, scraper plate rotating speed is to carry out molecular distillation under conditions of 80~150rpm, takes light component to be carried for rose Take thing.
The saccharomycete used in the present invention is saccharomyces cerevisiae (Saccharomyces cerevisiae) HHL-1, in 2009 It is preserved in Wuhan University's China typical culture collection center on November 17, in, deposit number is CCTCC NO:M 209272.
The immobilized microorganism technology that the present invention is used has that fermentation process efficiency high, separation of solid and liquid be easy, mithridatism is strong The advantages of, the deficiency of traditional biological technique can be made up.
The present invention, using immobilized yeast fermentation Flos Rosae Rugosae extract, obtains a kind of quality using rose as raw material Excellent cigarette Flos Rosae Rugosae extract, the extract has abundant cigarette perfume in cigarette, increases fragrant and sweet and floral notes, increase volume The cigarette perfume gas property sent out thoroughly, oral cavity pleasant impression is relatively comfortable.Compared with existing rose flower concrete and Flos Rosae Rugosae extract, the Flos Rosae Rugosae extract Fragrance more enriches in cigarette, and fresh and sweet note and the fragrance of a flower rhythm is more obvious, and the property sent out is more preferable thoroughly for cigarette flavor；With suspended yeast bacterium Fermentation is compared, and esters fragrance content of material adds 10.58%~14.96%, benzene in Flos Rosae Rugosae extract prepared by this method Ethanol content is added in 20.58%~28.42%, cigarette effect assessment, and fragrance more enriches, and fresh and sweet fragrant and fragrance of a flower rhythm is more Substantially, oral cavity pleasant impression is more comfortable.This method instead of the traditional extractive technique of Flos Rosae Rugosae extract, have updated biofermentation system Standby technique so that it applicability can increase in cigarette, and the preparation to cigarette Flos Rosae Rugosae extract provides a new way.
Embodiment
With reference to embodiment to further detailed description of the present invention.
PVA-SA (polyvinyl alcohol-sodium alginate) immobilized yeast bead in following examples of the present invention be according to Lower method is obtained using polyvinyl alcohol (PVA) and sodium alginate (SA) to being fixed of saccharomycete：
(1) saccharomycete is inoculated into broth bouillon, cultivated under conditions of 37 DEG C of temperature, speed of agitator are 50rpm 48 hours, take nutrient solution in being centrifuged under 8000rpm, the wet thallus after centrifugation is standby；It is conventional that agar culture is supported in the above method Broth medium, its Ju Ti Pei Fang be (every liter)：Peptone 10g, beef extract powder 3g, sodium chloride 5g, final pH 7.3 ± 0.1；
(2) polyvinyl alcohol-Immobilization in Sodium Alginate of saccharomycete：It is water-soluble with sodium alginate using polyvinyl alcohol water solution Liquid prepares the polyvinyl alcohol containing mass percent 6%~10% and the mixing of the sodium alginate of mass percent 1%~3% is molten The saccharomycete wet thallus prepared in liquid, the step of the 1~3% of total solution volume is then added into mixed solution (2), is configured to Mixed liquor, CaCl is added dropwise to peristaltic pump by the syringe needle in 1~5mm apertures by the mixed liquor prepared2Quality percentage Than in the boric acid saturated solution for 1~3%, then bead is taken out and is then added to saturation Na by crosslinking 1h formation beads2SO4Solution Middle crosslinking 3-5h, obtains PVA-SA immobilized yeast beads, then using aseptic water washing, dry for standby；It is described to contain 1-3% CaCl2Boric acid saturated solution amount and saturation Na2SO4The amount of solution has no particular/special requirement, contains 1-3%CaCl2Boric acid satisfy Only need to that mixed liquor can be made to produce precipitation with solution, meet the immobilization requirement of lactic acid bacteria, Na2SO4Solution is only needed to Bead is completely soaked and just may be used；The boric acid saturated solution, saturation Na2SO4Solution is saturated aqueous solution.
The saccharomycete used in the present invention is saccharomyces cerevisiae (Saccharomyces cerevisiae) HHL-1, in 2009 It is preserved in Wuhan University's China typical culture collection center on November 17, in, deposit number is CCTCC NO:M 209272.
Embodiment 1, a kind of method that immobilized yeast prepares cigarette Flos Rosae Rugosae extract, comprises the following steps：
(1) rose is taken, the water of 3 times of its weight is added, refluxing extraction 2 times at 50 DEG C, each extraction time is 2 small When, room temperature is cooled to after having extracted, and extract solution is filtrated to get, merge extract solution twice, obtain Flos Rosae Rugosae extract A, twice The condition of extraction is identical；
(2) the immobilized yeast bead for preparing the Flos Rosae Rugosae extract A prepared in step (1) with the above method according to Mass ratio 5:1 mixing, is 20 DEG C in temperature, speed of agitator is the 4d that fermented under conditions of 5rpm, is subsequently placed in 4 DEG C of refrigerator and protects Deposit 8d；
(3) step (2) fermentation after fermentation liquid is filtered, obtains clarified broth, the zymotic fluid after filtering is freeze-dried, The feed liquid thickness of freeze-drying is 1~2 centimetre, and vacuum is 10Pa, and drying time is 10h；
(4) zymotic fluid after step (3) is freeze-dried, adds 80% alcoholic solution of 2 times of its quality, and precipitation 24 is small When, it is 0.1MPa to filter to take supernatant in pressure, and temperature is that to be concentrated into relative density under conditions of 45 DEG C be 1.25, then dense Propane diols is added in contracting thing, Flos Rosae Rugosae extract B is obtained, the addition of the propane diols is the 20% of gross mass；
(5) by the Flos Rosae Rugosae extract B of step (4), in pressure 2Pa, feeding temperature is 55 DEG C, and charging rate is 20ml/ Min, scraper plate rotating speed is 80rpm, carries out molecular distillation, and it is Flos Rosae Rugosae extract to take light component.
The Flos Rosae Rugosae extract prepared in embodiment 1, which is added in cigarette, has abundant cigarette perfume, fresh and sweet note and the fragrance of a flower rhythm More obvious, the property sent out is more preferable thoroughly for cigarette perfume.
Embodiment 2, a kind of method that immobilized yeast prepares cigarette Flos Rosae Rugosae extract, comprises the following steps：
(1) rose is taken, the water of 5 times of its weight is added, refluxing extraction 2 times at 60 DEG C, each extraction time is 2.5 small When, room temperature is cooled to after having extracted, and extract solution is filtrated to get, merge extract solution twice, obtain Flos Rosae Rugosae extract A, twice The condition of extraction is identical；
(2) the immobilized yeast bead for preparing the Flos Rosae Rugosae extract A prepared in step (1) with the above method according to Mass ratio 7:1 mixing, is 23 DEG C in temperature, speed of agitator is the 3d that fermented under conditions of 4rpm, is subsequently placed in 4 DEG C of refrigerators and preserves 12d；
(3) step (2) fermentation after fermentation liquid is filtered, obtains clarified broth, the zymotic fluid after filtering is freeze-dried, Feed liquid thickness is 1~2 centimetre, and vacuum is 10Pa, and drying time is 8h；
(4) zymotic fluid after step (3) is freeze-dried, the concentration for adding 3 times of its quality is 85% alcoholic solution, precipitation 24 hours, it was 0.1MPa to filter to take supernatant in pressure, and temperature is that to be concentrated into relative density under conditions of 45 DEG C be 1.25, then Propane diols is added in concentrate, Flos Rosae Rugosae extract B is obtained, the addition of the propane diols is the 20% of gross mass；
(5) it is 5Pa in pressure, feeding temperature is 65 DEG C, and charging rate is by the Flos Rosae Rugosae extract B of step (4) 30ml/min, scraper plate rotating speed is carries out molecular distillation under conditions of 110rpm, and it is Flos Rosae Rugosae extract to take light component.
The Flos Rosae Rugosae extract prepared in embodiment 2, which is added in cigarette, has abundant cigarette perfume, fresh and sweet note and the fragrance of a flower rhythm More obvious, the property sent out is more preferable thoroughly for cigarette perfume.
Embodiment 3, a kind of method that immobilized yeast prepares cigarette Flos Rosae Rugosae extract, comprises the following steps：
(1) rose is taken, the water of 8 times of its weight is added, refluxing extraction 3 times at 65 DEG C, each extraction time is 3 small When, it is cooled to room temperature after having extracted, and be filtrated to get extract solution, merges No. three extract solutions, obtains Flos Rosae Rugosae extract A, three times The condition of extraction is identical；
(2) the immobilized yeast bead for preparing the Flos Rosae Rugosae extract A prepared in step (1) with the above method according to Mass ratio 10:1 mixing, is 25 DEG C in temperature, speed of agitator is the 2d that fermented under conditions of 1rpm, is subsequently placed in 4 DEG C of refrigerators and protects Deposit 7d；
(4) step (3) fermentation after fermentation liquid is filtered, obtains clarified broth, the zymotic fluid after filtering is freeze-dried, Feed liquid thickness is 1~2 centimetre, and vacuum is 10Pa, and drying time is 6h；
(5) zymotic fluid after step (4) is freeze-dried, adds the alcoholic solution of concentration 95% of 4 times of its quality, precipitation 48 Hour, it is 0.1MPa to filter to take supernatant in pressure, and temperature is that to be concentrated into relative density under conditions of 45 DEG C be 1.35, Ran Hou Propane diols is added in concentrate, Flos Rosae Rugosae extract B is obtained, the addition of the propane diols is the 20% of gross mass；
(6) by the extract B of step (5), in pressure 10Pa, feeding temperature is 70 DEG C, and charging rate is 50ml/min, is scraped Plate rotating speed is carries out molecular distillation under conditions of 150rpm, and it is Flos Rosae Rugosae extract to take light component.
The Flos Rosae Rugosae extract prepared in embodiment 3, which is added in cigarette, has abundant cigarette perfume, fresh and sweet note and the fragrance of a flower rhythm More obvious, the property sent out is more preferable thoroughly for cigarette perfume.
Contrast experiment, the rose that the Flos Rosae Rugosae extract that applicant prepares the present invention is prepared with existing traditional extraction process Flower extract, the Flos Rosae Rugosae extract prepared that fermented using suspended yeast bacterium are added in pipe tobacco according to normal perfuming mode, are made Into cigarette, and smoked panel test, its effect of smokeing panel test is as follows：
The sensory evaluation effect that rose prepared by three kinds of different process is extracted in cigarette compares
It can be seen that to come from above-mentioned contrast experiment, the Flos Rosae Rugosae extract for preparing is added in cigarette effect more in the present invention It is good, and found according to specific experiment analysis, compared with suspended yeast bacterium is fermented, ester in Flos Rosae Rugosae extract prepared by this method Class Aromatic Matter Contents add 10.58%~14.96%, and benzyl carbinol content adds 20.58%~28.42%, cigarette effect During fruit is evaluated, fragrance more enriches, and fresh and sweet fragrant and fragrance of a flower rhythm is more obvious, and oral cavity pleasant impression is more comfortable.

Claims (2)

1. a kind of method that immobilized yeast prepares cigarette Flos Rosae Rugosae extract, it is characterised in that specifically include following steps：
(1) rose is taken, the water of 3~8 times of its weight is added, Microwave Extraction 2~3 times at 50~65 DEG C, each extraction time For 2~3 hours, room temperature is cooled to after having extracted, and is filtrated to get extract solution, merges multiple extract solution, obtains rose extraction Thing A；
(2) saccharomycete is inoculated into broth bouillon, culture 48 is small under conditions of 37 DEG C of temperature, speed of agitator are 50rpm When, take nutrient solution in being centrifuged under 8000rpm, the wet thallus after centrifugation is standby；
(3) polyvinyl alcohol-Immobilization in Sodium Alginate of saccharomycete：Prepared using polyvinyl alcohol water solution and sodium alginate aqueous solution The mixed solution of the sodium alginate of polyvinyl alcohol and mass percent 1%~3% containing mass percent 6%~10%, then The saccharomycete wet thallus prepared in the step of the 1~3% of total solution volume is added into mixed solution (2), is configured to mixed liquor, The mixed liquor prepared is added dropwise to CaCl by the syringe needle in 1~5mm apertures with peristaltic pump2Mass percent for 1~ In 3% boric acid saturated solution, then bead is taken out and adds saturation Na by crosslinking 1h formation beads2SO43- is crosslinked in solution 5h, obtains PVA-SA immobilized yeast beads, then using aseptic water washing, dry for standby；
(4) by the immobilized yeast bead prepared in the Flos Rosae Rugosae extract A and step (3) that are prepared in step (1) according to matter Measure ratio 5~10:1 mixing, is 20~25 DEG C in temperature, and speed of agitator is 2~4d of fermentation under conditions of 1~5rpm, is subsequently placed in 4 7~14d is preserved in DEG C refrigerator；
(5) step (4) fermentation after fermentation liquid is filtered, obtains clarified broth, zymotic fluid is freeze-dried, feed liquid thickness is 1 ~2 centimetres, vacuum is 10Pa, and drying time is 6~12h；
(6) zymotic fluid after step (5) freeze-drying is taken, 80~95% alcoholic solutions of 2~4 times of its quality, precipitation 24 are added ~48 hours, it was 0.1MPa to filter to take supernatant in pressure, temperature be concentrated under conditions of 45 DEG C relative density be 1.25~ 1.35, propane diols is then added in concentrate, Flos Rosae Rugosae extract B is obtained, the addition of the propane diols is gross mass 20%；
(7) by the Flos Rosae Rugosae extract B of step (6), in 1~10Pa of pressure, feeding temperature is 55 DEG C~70 DEG C, and charging rate is 20~50ml/min, scraper plate rotating speed is carries out molecular distillation under conditions of 80~150rpm, and it is Flos Rosae Rugosae extract to take light component.
2. the method that a kind of immobilized yeast according to claim 1 prepares cigarette Flos Rosae Rugosae extract, its feature exists Saccharomycete in the step (2) is saccharomyces cerevisiae (Saccharomyces cere visiae) HHL-1, and deposit number is CCTCC NO:M 209272。
CN201610899648.6A 2016-10-13 2016-10-13 A kind of method that immobilized yeast prepares cigarette Flos Rosae Rugosae extract Pending CN106957839A (en)

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Cited By (3)

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CN108185432A (en) * 2017-12-31 2018-06-22 西双版纳天恩生物科技开发有限公司 The preparation method of Flos Rosae Rugosas pollen is lyophilized
CN108822964A (en) * 2018-06-21 2018-11-16 湖北中烟工业有限责任公司 A kind of preparation method of cigarette raisins distillation
CN110946319A (en) * 2019-11-20 2020-04-03 许达勇 Nicotine propellant and preparation method thereof

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CN108185432A (en) * 2017-12-31 2018-06-22 西双版纳天恩生物科技开发有限公司 The preparation method of Flos Rosae Rugosas pollen is lyophilized
CN108822964A (en) * 2018-06-21 2018-11-16 湖北中烟工业有限责任公司 A kind of preparation method of cigarette raisins distillation
CN108822964B (en) * 2018-06-21 2021-06-15 湖北中烟工业有限责任公司 Preparation method of raisin distillate for cigarettes
CN110946319A (en) * 2019-11-20 2020-04-03 许达勇 Nicotine propellant and preparation method thereof

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Application publication date: 20170718