CN106434829B - Fermented rose extract for cigarettes and preparation method thereof - Google Patents
Fermented rose extract for cigarettes and preparation method thereof Download PDFInfo
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- CN106434829B CN106434829B CN201611100820.3A CN201611100820A CN106434829B CN 106434829 B CN106434829 B CN 106434829B CN 201611100820 A CN201611100820 A CN 201611100820A CN 106434829 B CN106434829 B CN 106434829B
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- 241000220317 Rosa Species 0.000 title claims abstract description 110
- 235000019504 cigarettes Nutrition 0.000 title claims abstract description 25
- 238000002360 preparation method Methods 0.000 title claims description 11
- 239000007788 liquid Substances 0.000 claims abstract description 43
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims abstract description 40
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 37
- 235000002247 Aspergillus oryzae Nutrition 0.000 claims abstract description 36
- 240000006439 Aspergillus oryzae Species 0.000 claims abstract description 36
- 239000001963 growth medium Substances 0.000 claims abstract description 18
- 241000628997 Flos Species 0.000 claims abstract description 15
- 108010059892 Cellulase Proteins 0.000 claims abstract description 14
- 229940106157 cellulase Drugs 0.000 claims abstract description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 13
- 238000000855 fermentation Methods 0.000 claims description 24
- 230000004151 fermentation Effects 0.000 claims description 23
- DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical compound CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 claims description 15
- 239000006228 supernatant Substances 0.000 claims description 15
- 241000698291 Rugosa Species 0.000 claims description 10
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 10
- 239000000706 filtrate Substances 0.000 claims description 10
- 239000008223 sterile water Substances 0.000 claims description 10
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 6
- 238000000605 extraction Methods 0.000 claims description 6
- 239000008103 glucose Substances 0.000 claims description 6
- 238000001914 filtration Methods 0.000 claims description 5
- 239000012530 fluid Substances 0.000 claims description 5
- 239000002609 medium Substances 0.000 claims description 5
- 239000012982 microporous membrane Substances 0.000 claims description 5
- 235000015097 nutrients Nutrition 0.000 claims description 5
- 238000010992 reflux Methods 0.000 claims description 5
- 239000011780 sodium chloride Substances 0.000 claims description 5
- 230000001954 sterilising effect Effects 0.000 claims description 5
- 230000003519 ventilatory effect Effects 0.000 claims description 5
- 238000006243 chemical reaction Methods 0.000 claims description 4
- 238000001976 enzyme digestion Methods 0.000 claims description 4
- 238000012549 training Methods 0.000 claims description 4
- 229940050561 matrix product Drugs 0.000 claims 1
- 239000003205 fragrance Substances 0.000 abstract description 9
- 238000004519 manufacturing process Methods 0.000 abstract description 3
- 230000002255 enzymatic effect Effects 0.000 abstract description 2
- 238000002955 isolation Methods 0.000 abstract description 2
- UGFAIRIUMAVXCW-UHFFFAOYSA-N Carbon monoxide Chemical compound [O+]#[C-] UGFAIRIUMAVXCW-UHFFFAOYSA-N 0.000 description 2
- GLZPCOQZEFWAFX-UHFFFAOYSA-N Geraniol Chemical compound CC(C)=CCCC(C)=CCO GLZPCOQZEFWAFX-UHFFFAOYSA-N 0.000 description 2
- QMVPMAAFGQKVCJ-UHFFFAOYSA-N citronellol Chemical compound OCCC(C)CCC=C(C)C QMVPMAAFGQKVCJ-UHFFFAOYSA-N 0.000 description 2
- 239000000796 flavoring agent Substances 0.000 description 2
- 235000019634 flavors Nutrition 0.000 description 2
- 239000003546 flue gas Substances 0.000 description 2
- 210000000867 larynx Anatomy 0.000 description 2
- CDOSHBSSFJOMGT-UHFFFAOYSA-N linalool Chemical compound CC(C)=CCCC(C)(O)C=C CDOSHBSSFJOMGT-UHFFFAOYSA-N 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 239000002304 perfume Substances 0.000 description 2
- 235000019719 rose oil Nutrition 0.000 description 2
- 239000010666 rose oil Substances 0.000 description 2
- CZCBTSFUTPZVKJ-UHFFFAOYSA-N rose oxide Chemical compound CC1CCOC(C=C(C)C)C1 CZCBTSFUTPZVKJ-UHFFFAOYSA-N 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 239000001490 (3R)-3,7-dimethylocta-1,6-dien-3-ol Substances 0.000 description 1
- QMVPMAAFGQKVCJ-SNVBAGLBSA-N (R)-(+)-citronellol Natural products OCC[C@H](C)CCC=C(C)C QMVPMAAFGQKVCJ-SNVBAGLBSA-N 0.000 description 1
- CDOSHBSSFJOMGT-JTQLQIEISA-N (R)-linalool Natural products CC(C)=CCC[C@@](C)(O)C=C CDOSHBSSFJOMGT-JTQLQIEISA-N 0.000 description 1
- GLZPCOQZEFWAFX-YFHOEESVSA-N Geraniol Natural products CC(C)=CCC\C(C)=C/CO GLZPCOQZEFWAFX-YFHOEESVSA-N 0.000 description 1
- 239000005792 Geraniol Substances 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 244000061176 Nicotiana tabacum Species 0.000 description 1
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 1
- 235000011449 Rosa Nutrition 0.000 description 1
- 235000004789 Rosa xanthina Nutrition 0.000 description 1
- 241000220222 Rosaceae Species 0.000 description 1
- 244000269722 Thea sinensis Species 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 238000013475 authorization Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- JGQFVRIQXUFPAH-UHFFFAOYSA-N beta-citronellol Natural products OCCC(C)CCCC(C)=C JGQFVRIQXUFPAH-UHFFFAOYSA-N 0.000 description 1
- 210000004204 blood vessel Anatomy 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 235000000484 citronellol Nutrition 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000007071 enzymatic hydrolysis Effects 0.000 description 1
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 description 1
- 229940113087 geraniol Drugs 0.000 description 1
- 125000002791 glucosyl group Chemical group C1([C@H](O)[C@@H](O)[C@H](O)[C@H](O1)CO)* 0.000 description 1
- 230000002218 hypoglycaemic effect Effects 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 230000007794 irritation Effects 0.000 description 1
- 229930007744 linalool Natural products 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229930003658 monoterpene Natural products 0.000 description 1
- 235000002577 monoterpenes Nutrition 0.000 description 1
- -1 monoterpenes compound Chemical class 0.000 description 1
- 230000001151 other effect Effects 0.000 description 1
- 125000001997 phenyl group Chemical class [H]C1=C([H])C([H])=C(*)C([H])=C1[H] 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 229930007790 rose oxide Natural products 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- RXHIKAIVEMAPRU-JRIGQVHBSA-N sequiterpene Natural products C1=C(C)[C@@H](OC(C)=O)[C@H](O)[C@@]2(O)[C@H](C)CC[C@@H](C(C)=C)[C@H]21 RXHIKAIVEMAPRU-JRIGQVHBSA-N 0.000 description 1
- 229930004725 sesquiterpene Natural products 0.000 description 1
- 150000004354 sesquiterpene derivatives Chemical class 0.000 description 1
- 235000013599 spices Nutrition 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P39/00—Processes involving microorganisms of different genera in the same process, simultaneously
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B9/00—Essential oils; Perfumes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
- C12P1/02—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using fungi
Abstract
The present invention discloses a kind of fermented rose extract for cigarettes, it is that rose wate extract and rose residue enzymolysis liquid are mixed and made into rose spent culture medium, aspergillus oryzae and saccharomyces cerevisiae are made to ferment in seed liquor addition rose spent culture medium are prepared；The rose residue enzymolysis liquid is that cellulase is added into rose residue liquid；The rose residue liquid is to prepare the filter residue obtained after rose wate extract, and water is added into filter residue and obtains.Flos Rosae Rugosae extract is prepared in conjunction with enzymatic isolation method, not only makes full use of the residue of rose, reduces production cost, and the fragrance matter of rose can be made more fully to discharge, fragrance is sufficient.
Description
Technical field
The present invention relates to the application of microbial fermentation technology, specifically a kind of fermented rose extract for cigarettes and
Preparation method.
Background technique
Rose is rosaceae Rosa fallen leaves bush, and rose also known as flower of hovering, thorn rose, nib are spent, rose
In the main component of natural rose oil that contains be monoterpenes compound, as linalool, geraniol, citronellol and rose oxide,
Sesquiterpene, sequiterpene oxygenatedchemicals and benzene derivative, not only smell is pleasant but also anti-oxidant for this natural rose oil, resists swollen
Tumor expands blood vessel, hypoglycemic etc. and other effects.Nowadays, the cigarette of different taste increasingly receives the favor of consumer, particularly with
For women cigarette consumption person, have the shortcomings that the cigarette of the fragrance of a flower not only improves cigarette thorn larynx and the flowery odour of faint scent makes one
It is in a cheerful frame of mind.
Chinese invention patent (Authorization Notice No. CN102406231 B authorized announcement date 2013.12.11) discloses a kind of hair
Ferment type cigarette Flos Rosae Rugosae extract and preparation method thereof.It is glucose to be added in rose wate extract culture medium is made, will
Deposit number is the training of CCTCC NO:M209272, the entitled Saccharomyces Cerevisiae in S accharomyces cerevisiae HHL-1 of preservation
It supports object and culture solution is made, then will carry out fermentation in culture medium merging culture solution and be prepared.Fermented type prepared by the invention
Cigarette Flos Rosae Rugosae extract has strong Rose Essentielle, and has pleasant sour-sweet fragrance, is added in cigarette, with rich
Rich cigarette perfume, the effect for increasing sweet tea sense, reducing irritation and soft flue gas.But Saccharomyces Cerevisiae in S accharomyces
Cerevisiae HHL-1 fermentation can generate a large amount of wine flavour, and this wine flavour is strong, masks rose wate extract
Faint scent, and prepare rose wate extract and have a large amount of rose residue, contain a large amount of protein in rose residue, forms sediment
How powder, cellulose and various minerals will largely economize on resources these residues recycling, and reduction is produced into
This.
Summary of the invention
The purpose of the present invention is to drawbacks described above, a kind of fermented rose extract for cigarettes and its preparation side are provided
Method, obtained fermented rose extract for cigarettes not only reduce the stimulation to throat's nose and have apparent rose
Perfume (or spice), the preparation process is simple, reduces production cost.
To achieve the above object, the technical scheme is that
A kind of fermented rose extract for cigarettes, it is to mix rose wate extract with rose residue enzymolysis liquid
Rose spent culture medium is made, seed liquor is made in aspergillus oryzae and saccharomyces cerevisiae is added in rose spent culture medium to ferment and be prepared；
The rose residue enzymolysis liquid is that cellulase is added into rose residue liquid；The rose residue liquid is to prepare rose
The filter residue obtained after flower water extract, and water is added into filter residue and obtains.
Preferably, the cellulase accounts for the 0.05~0.1% of rose residue liquid weight, the aspergillus oryzae and wine brewing ferment
The total amount of aspergillus oryzae and saccharomyces cerevisiae is 1 × 10 in female seed liquor8~3 × 108cfu/mL。
A kind of preparation method of fermented rose extract for cigarettes, comprising the following steps:
1) rose is dried, is ground into Flos Rosae Rugosas pollen, take 100g Flos Rosae Rugosas pollen that 900g sterile water, refluxing extraction 4 is added
~5h, 0.2 μm of filtering with microporous membrane collect filtrate and filter residue；Filtrate is rose wate extract, and filter residue carries out at next step
Reason；
2) sterile water that 9 times of weight are added into filter residue obtains rose residue liquid, and controlling pH is 5.0~6.5, Xiang Mei
Cellulase is added in rare colored residue liquid, is centrifuged after enzyme digestion reaction, supernatant is taken to obtain rose residue enzymolysis liquid；
3) the rose wate extract that step 1) obtains is mixed with the rose residue enzymolysis liquid that step 2) obtains, then plus
Enter 0.05~0.50% sodium chloride and 0.08~0.2% glucose sugar by high-pressure sterilizing pot handle be made rose spent culture medium,
The pH for controlling rose spent culture medium is 6.5~7.5；
4) by aspergillus oryzae and saccharomyces cerevisiae strain access YPD fluid nutrient medium, constant temperature shaker temperature is 25~30 DEG C, is turned
Speed is 150~160rpm, and culture obtained aspergillus oryzae and saccharomyces cerevisiae seed liquor after 1~2 day；
5) aspergillus oryzae and saccharomyces cerevisiae seed liquor are added into rose spent culture medium, ferments in the fermenter；
6) after fermenting, it is centrifuged fermentation liquid, takes supernatant, supernatant is concentrated to get concentration fermentation with Rotary Evaporators
Liquid adds the propylene glycol that weight percent is 2~20% into concentrated broth and is extracted to get to fermentation type tobacco with rose
Object.
Preferably, the step 2) cellulase accounts for the 0.05~0.1% of rose residue liquid weight, and enzymolysis time is
2~3h；Aspergillus oryzae and the total amount of aspergillus oryzae and saccharomyces cerevisiae in saccharomyces cerevisiae seed liquor are 1 × 10 in the step 4)8~3 ×
108cfu/mL；Zymotechnique in the step 5) are as follows: pH value is 6.0~8.0, and speed of agitator is 250~300rpm, ventilatory capacity
For 6~8VVM, fermentation temperature is 25 DEG C~30 DEG C, and fermentation time is 2d~4d；Aspergillus oryzae and saccharomyces cerevisiae in the step 5)
The volume of seed liquor is the 5~10% of rose culture volume, and the temperature of Rotary Evaporators is 70~80 in the step 6)
DEG C, revolving speed is 120~140rpm, and the time is 1~2h.
Cellulase, trypsase and the pectase that the present invention uses are purchased from Wuhan Biological Products Inst., aspergillus oryzae
Kind is purchased from Wuhan University's Culture Collection Center, and saccharomyces cerevisiae is the saccharomyces cerevisiae in patent publication No. 102406231A
Saccharomyces cerevisiae HHL-1, in be Rotary Evaporators model BUCHIR-3000 type.
The beneficial effects of the present invention are:
1, the present invention uses aspergillus oryzae and saccharomyces cerevisiae co-fermentation, and compared to saccharomyces cerevisiae is utilized merely, fragrance is clear
It is light, the fragrance of a flower of rose, and the soft rose fragrance of a flower of energy will not be covered, the not pungent larynx of cigarette Flos Rosae Rugosae extract, flue gas are made
It is more elegant.
2, Flos Rosae Rugosae extract is prepared in conjunction with enzymatic isolation method, not only makes full use of the residue of rose, reduces production cost,
And the fragrance matter of rose can be made more fully to discharge, fragrance is sufficient.
Specific embodiment
For a better understanding of the invention, invention is described in detail in conjunction with specific example.
Embodiment 1
The preparation method of fermented rose extract for cigarettes, comprising the following steps:
1) rose is dried, is ground into Flos Rosae Rugosas pollen, take 100g Flos Rosae Rugosas pollen that 900g sterile water, refluxing extraction is added
4h, 0.2 μm of filtering with microporous membrane collect filtrate and filter residue；Filtrate is rose wate extract, and filter residue is further processed；
2) sterile water that 9 times of weight are added into filter residue obtains rose residue liquid, and controlling pH is 5.0, to rose
Cellulase is added in residue liquid, cellulase accounts for the 0.05% of rose residue liquid weight, enzymolysis time 3h；Enzyme digestion reaction
After be centrifuged, take supernatant to obtain rose residue enzymolysis liquid；
3) the rose wate extract that step 1) obtains is mixed with the rose residue enzymolysis liquid that step 2) obtains, then plus
Enter 0.05% sodium chloride and 0.08% glucose sugar by high-pressure sterilizing pot handle be made rose spent culture medium, control rose training
The pH for supporting base is 6.5；
4) by aspergillus oryzae and saccharomyces cerevisiae strain access YPD fluid nutrient medium, constant temperature shaker temperature is 25 DEG C, and revolving speed is
160rpm, culture obtain aspergillus oryzae and saccharomyces cerevisiae seed liquor after 2 days, aspergillus oryzae and wine in aspergillus oryzae and saccharomyces cerevisiae seed liquor
The total amount of brewer yeast is 1 × 108cfu/mL；
5) aspergillus oryzae and saccharomyces cerevisiae seed liquor, aspergillus oryzae and saccharomyces cerevisiae seed liquor are added into rose spent culture medium
Volume is the 10% of rose culture volume；It ferments in the fermenter, zymotechnique are as follows: pH value 6.0, speed of agitator are
250rpm, ventilatory capacity 6VVM, fermentation temperature are 25 DEG C DEG C, fermentation time 2d；
6) after fermenting, it is centrifuged fermentation liquid, takes supernatant, supernatant is concentrated to get concentration fermentation with Rotary Evaporators
Liquid, the temperature of Rotary Evaporators are 70 DEG C, revolving speed 140rpm, time 2h；Weight percent is added into concentrated broth
For 2% propylene glycol to get arrive fermented rose extract for cigarettes.
Embodiment 2
The preparation method of fermented rose extract for cigarettes, comprising the following steps:
1) rose is dried, is ground into Flos Rosae Rugosas pollen, take 100g Flos Rosae Rugosas pollen that 900g sterile water, refluxing extraction is added
5h, 0.2 μm of filtering with microporous membrane collect filtrate and filter residue；Filtrate is rose wate extract, and filter residue is further processed；
2) sterile water that 9 times of weight are added into filter residue obtains rose residue liquid, and controlling pH is 6.5, to rose
Cellulase is added in residue liquid, cellulase accounts for the 0.1% of rose residue liquid weight, enzymolysis time 2h；Enzyme digestion reaction
After be centrifuged, take supernatant to obtain rose residue enzymolysis liquid；
3) the rose wate extract that step 1) obtains is mixed with the rose residue enzymolysis liquid that step 2) obtains, then plus
Enter 0.50% sodium chloride and 0.2% glucose sugar by high-pressure sterilizing pot handle be made rose spent culture medium, control rose training
The pH for supporting base is 7.5；
4) by aspergillus oryzae and saccharomyces cerevisiae strain access YPD fluid nutrient medium, constant temperature shaker temperature is 30 DEG C, and revolving speed is
150rpm, culture obtain aspergillus oryzae and saccharomyces cerevisiae seed liquor after 1 day, aspergillus oryzae and wine in aspergillus oryzae and saccharomyces cerevisiae seed liquor
The total amount of brewer yeast is 3 × 108cfu/mL；
5) aspergillus oryzae and saccharomyces cerevisiae seed liquor, aspergillus oryzae and saccharomyces cerevisiae seed liquor are added into rose spent culture medium
Volume is the 5% of rose culture volume；It ferments in the fermenter, zymotechnique are as follows: pH value 8.0, speed of agitator are
300rpm, ventilatory capacity 8VVM, fermentation temperature are 30 DEG C, fermentation time 4d；
6) after fermenting, it is centrifuged fermentation liquid, takes supernatant, supernatant is concentrated to get concentration fermentation with Rotary Evaporators
Liquid, the temperature of Rotary Evaporators are 80 DEG C, revolving speed 120rpm, time 1h；Weight percent is added into concentrated broth
For 20% propylene glycol to get arrive fermented rose extract for cigarettes.
Embodiment 3
The preparation method of fermented rose extract for cigarettes, comprising the following steps:
1) rose is dried, is ground into Flos Rosae Rugosas pollen, take 100g Flos Rosae Rugosas pollen that 900g sterile water, refluxing extraction is added
4.5h, 0.2 μm of filtering with microporous membrane collect filtrate and filter residue；Filtrate is rose wate extract, and filter residue carries out at next step
Reason；
2) sterile water that 9 times of weight are added into filter residue obtains rose residue liquid, and controlling pH is 6.0, to rose
Cellulase is added in residue liquid, cellulase accounts for the 0.08% of rose residue liquid weight, enzymolysis time 2.5h；Enzymatic hydrolysis is anti-
It is centrifuged after answering, supernatant is taken to obtain rose residue enzymolysis liquid；
3) the rose wate extract that step 1) obtains is mixed with the rose residue enzymolysis liquid that step 2) obtains, then plus
Enter 0.2% sodium chloride and 0.1% glucose sugar by high-pressure sterilizing pot handle be made rose spent culture medium, control rose culture
The pH of base is 7.0；
4) by aspergillus oryzae and saccharomyces cerevisiae strain access YPD fluid nutrient medium, constant temperature shaker temperature is 28 DEG C, and revolving speed is
155rpm, culture obtain aspergillus oryzae and saccharomyces cerevisiae seed liquor after 2 days, aspergillus oryzae and wine in aspergillus oryzae and saccharomyces cerevisiae seed liquor
The total amount of brewer yeast is 2 × 108cfu/mL；
5) aspergillus oryzae and saccharomyces cerevisiae seed liquor, aspergillus oryzae and saccharomyces cerevisiae seed liquor are added into rose spent culture medium
Volume is the 8% of rose culture volume；It ferments in the fermenter, zymotechnique are as follows: pH value 7.0, speed of agitator are
280rpm, ventilatory capacity 7VVM, fermentation temperature are 28 DEG C, fermentation time 3d；
6) after fermenting, it is centrifuged fermentation liquid, takes supernatant, supernatant is concentrated to get concentration fermentation with Rotary Evaporators
Liquid, the temperature of Rotary Evaporators are 75 DEG C, revolving speed 130rpm, time 1.5h；Weight percent is added into concentrated broth
Than the propylene glycol for 10% to get arrive fermented rose extract for cigarettes.
Examples 1 to 3 is subjected to test of smokeing panel test, the results are shown in Table 1:
Table 1
The embodiments described above only express several embodiments of the present invention, and the description thereof is more specific and detailed, but simultaneously
Limitations on the scope of the patent of the present invention therefore cannot be interpreted as.It should be pointed out that for those of ordinary skill in the art
For, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to guarantor of the invention
Protect range.Therefore, the scope of protection of the patent of the invention shall be subject to the appended claims.
Claims (1)
1. a kind of preparation method of fermented rose extract for cigarettes, comprising the following steps:
1) rose is dried, is ground into Flos Rosae Rugosas pollen, take 100g Flos Rosae Rugosas pollen be added 900g sterile water, 4 ~ 5h of refluxing extraction,
0.2 μm of filtering with microporous membrane collects filtrate and filter residue；Filtrate is rose wate extract, and filter residue is further processed；
2) sterile water that 9 times of weight are added into filter residue obtains rose residue liquid, and controlling pH is 5.0 ~ 6.5, to rose
Cellulase is added in residue liquid, is centrifuged after enzyme digestion reaction, supernatant is taken to obtain rose residue enzymolysis liquid；
3) the rose wate extract that step 1) obtains is mixed with the rose residue enzymolysis liquid that step 2 obtains, is added
0.05 ~ 0.50% sodium chloride and 0.08 ~ 0.2% glucose by high-pressure sterilizing pot handle be made rose spent culture medium, control rose
The pH of rare spent culture medium is 6.5 ~ 7.5；
4) by aspergillus oryzae and saccharomyces cerevisiae strain access YPD fluid nutrient medium, constant temperature shaker temperature is 25 ~ 30 DEG C, and revolving speed is
150 ~ 160rpm, culture obtained aspergillus oryzae and saccharomyces cerevisiae seed liquor after 1 ~ 2 day；
5) aspergillus oryzae and saccharomyces cerevisiae seed liquor are added into rose spent culture medium, ferments in the fermenter；
6) after fermenting, it is centrifuged fermentation liquid, supernatant is taken, supernatant is concentrated to get concentrated broth with Rotary Evaporators,
The propylene glycol that weight percent is 2 ~ 20% is added into concentrated broth to get fermented rose extract for cigarettes is arrived；
The step 2 cellulase accounts for the 0.05 ~ 0.1% of rose residue liquid weight, and enzymolysis time is 2 ~ 3h；The step
4) aspergillus oryzae and the total amount of aspergillus oryzae and saccharomyces cerevisiae in saccharomyces cerevisiae seed liquor are 1 × 10 in8~3×108cfu/mL；The step
It is rapid 5) in zymotechnique are as follows: pH value is 6.0 ~ 8.0, and speed of agitator is 250 ~ 300rpm, and ventilatory capacity is 6 ~ 8VVM, and fermentation temperature is
25 DEG C ~ 30 DEG C, fermentation time is 2d ~ 4d；The volume of aspergillus oryzae and saccharomyces cerevisiae seed liquor is rose training in the step 5)
The 5 ~ 10% of matrix product are supported, the temperature of Rotary Evaporators is 70 ~ 80 DEG C in the step 6), and revolving speed is 120 ~ 140rpm, and the time is
1~2h。
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| CN106434829B true CN106434829B (en) | 2019-09-10 |
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| CN102776072A (en) * | 2012-07-09 | 2012-11-14 | 广州市澳键丰泽生物科技有限公司 | Method for preparing rose essential oil |
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