CN107630045A - A kind of tobacco aromaticss prepared using microbial fermentation butterflybush flower and its application - Google Patents
A kind of tobacco aromaticss prepared using microbial fermentation butterflybush flower and its application Download PDFInfo
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- CN107630045A CN107630045A CN201710830995.8A CN201710830995A CN107630045A CN 107630045 A CN107630045 A CN 107630045A CN 201710830995 A CN201710830995 A CN 201710830995A CN 107630045 A CN107630045 A CN 107630045A
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- butterflybush flower
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- tobacco aromaticss
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Abstract
The invention discloses a kind of tobacco aromaticss prepared using microbial fermentation butterflybush flower and its application.Using butterflybush flower as raw material, cave gemmule bacterium SYSUZL 2 is cultivated into after fermentation butterflybush flower through expanding, after 30 DEG C of 24~48h of shaken cultivation, then tobacco aromaticss are obtained after refluxing extraction, filter cleaner, supernatant are concentrated under reduced pressure.Bacterial strain deposit number CCTCC M2016739.Show that described cave gemmule bacterium is microorganism novel species by Microbiological Characteristics analysis and 16S rRNA gene sequencings.The bacterial strain and the product of butterflybush flower co-fermentation are compared with untreated control, and cigarette sweet sense increase, flue gas conglobation property improves, the rich increase of fragrance.
Description
Technical field
The invention belongs to microbe application and tobacco aromaticss preparing technical field, is specifically related to one kind and utilizes microorganism
The tobacco aromaticss that novel species (cave gemmule bacterium) is prepared with Special plant (butterflybush flower) co-fermentation.Meanwhile the present invention also relates to
And application of the described tobacco aromaticss in production of cigarettes.
Background technology
Microbe species enrich, and for a long time, people carry out investigation to the microorganism in environment using pure culture technigne and ground
Study carefully and develop.With the development of science and technology, importance of the microbial resources in Technological research and biological industry is increasingly
Highlight.Many countries are all collecting, are preserving various microbial resources in the world, and it is enriched constantly for the development of biotechnology
Establish solid material base.
Flavoring and casing is the key link in cigarette process, is effective lifting cigarette smoking quality, forms the peculiar wind of cigarette
One of effective means of lattice.The tobacco aromaticss added at present in China tobacco are adjusted again using conventional method extraction mostly
With gained, although production operation is simple, there is also fragrance dullness, improve the shortcomings of cigarette smoking DeGrain.Using micro-
Biology ferments to perfume material, can increase the content of a variety of aroma substances in tradition extraction spices, and lifting fragrance enriches
Property.And existing industrial aroma-producing microbe has the problems such as species solidification, fermentation fragrance type is limited, there is an urgent need to find and sieve
New can the fermenting of choosing produces the microbial strains of special aroma, to expand the rich of tobacco aromaticss, meets production of cigarettes need
Ask.
Butterflybush flower, also known as dye meal flower, kamuning, native brocade flower, illiteracy flower, yellow meal flower, pimple skin tree, chicken bone flower, are horse
Money section Lindley Butterflybush Herb category shrub, high 1-4 rice.Sprig is slightly in four prismatics, taupe；The close quilt of sprig, adaxial surface of the leaf, petiole and inflorescence
The starlike down of canescence, it is the drying flower or bud of loganiaceae plant butterflybush flower, there is wind-dispelling, cool blood, profit liver, the work of improving eyesight
With red eye, swell pain, more tear photophobia, glaucoma ocular, the rotten eye of wind string can be controlled.Raw height above sea level 200-2800 rice endroit, river bank, village
In other bushes or border, China, Bhutan, Burma, Vietnam are distributed in.
Domestic and international tobacco worker has done many work in terms of tobacco aromaticss are produced using microbial fermentation, but
It is limited to aroma-producing yeast more.Cave gemmule bacterium provided by the invention obtains to be separated first, is identified as microorganism novel species, mesh
It is preceding not there is any disclosure on the cave gemmule bacterium and related application to report.
The content of the invention
Prepared in view of the above-mentioned deficiencies in the prior art, it is an object of the present invention to provide one kind using microbial fermentation butterflybush flower
Obtained tobacco aromaticss.
The present invention also aims to provide concrete application of the described tobacco aromaticss in production of cigarettes.
The purpose of the present invention is achieved by the following technical programs.
Unless otherwise indicated, percentage of the present invention is mass percent.
A kind of tobacco aromaticss prepared using microbial fermentation butterflybush flower：Using butterflybush flower as raw material, with cave gemmule bacterium
SYSUZL-2 (Blastococcus cavernae sp.SYSUZL-2) is fermented, then by extracting aroma component in zymotic fluid
Produce product；Described cave gemmule bacterium SYSUZL-2, is preserved in China typical culture collection center, preservation date 2016
On December 9, in, deposit number CCTCC NO： M 2016739.
Described cave gemmule bacterium (Blastococcus cavernae sp.) SYSUZL-2, in the noise made in coughing or vomiting that vega is adjoining
It is isolated in this special cave soil, volatility peat-reek breath can be produced on the culture medium containing simple carbon nitrogen source.Through
Cross Microbiological Characteristics analysis and 16S rRNA gene sequencings show that the bacterial strain is a kind of new bacterium.The bacterial strain can
Produce a few class main volatile compounds such as citronellol, geraniol and baras camphor, terpineol, linalool.Wherein, baras camphor is clinical
On have the effect of anti-inflammatory analgesic, there is linalool fresh and sweet breath to be cleaned available for perfuming cigarette, and citronellol has antimycotic, anti-
Oxidation activity, pooled applications are in terms of medicine.Terpineol can be used as food essence and cosmetics substrate, with extensive.Utilize
The strain fermentation, can obtain the tobacco aromaticss of special aroma, and gained spices fragrance enriches full, can enrich cigarette perfume, strengthen
Cigarette style.
The described tobacco aromaticss prepared using microbial fermentation butterflybush flower, are obtained especially by following methods：
(1) inclined-plane culture：Cave gemmule bacterium SYSUZL-2 is inoculated with slant medium, 28 DEG C of cultivation temperature, during culture
Between 7d；Described slant medium is：Glucose 0.5g, yeast extract 0.5g, peptone 0.5g, acid hydrolyzed casein 0.5g, can
Soluble starch 0.5g, Sodium Pyruvate 0.3g, dipotassium hydrogen phosphate 0.3g, magnesium sulfate 0.05g, agar 15g and distilled water 1000ml,
Adjust pH value 7.2；
(2) seed culture：Accessed from inclined-plane picking mycelium in seed culture medium, Shaking culture 36 hours, cultivation temperature
28 DEG C, obtain activation bacterium solution；Described seed culture medium is：Dextrin 120g, soy meal 40g, yeast extract 2g, tryptophan 0.5g, β-
Alanine 5g, magnesium sulfate 0.5g, ammonium phosphate 0.2g and distilled water 1000ml, adjust pH value 7.2；
(3) ferment：After butterflybush flower is crushed add double weight water, be well mixed, by butterflybush flower weight 5%~
20% inoculation cave gemmule bacterium activation bacterium solution, shaken cultivation are fermented, 30 DEG C of fermentation temperature, 24~48h of fermentation time；
(4) spices extracts：Fermentation liquor backflow extraction 3h, filter cleaner obtained by step (3) are taken, takes supernatant, 50 DEG C of decompressions
Medicinal extract is condensed into, produces required tobacco aromaticss.
Application of the described tobacco aromaticss in production of cigarettes, it is specially：Equably sprayed after gained tobacco aromaticss are diluted
It is sprinkled upon on pipe tobacco, the addition of tobacco aromaticss is the 0.2%~0.6% of pipe tobacco weight.
Relative to prior art, the present invention has advantages below：
(1) cave gemmule bacterium is applied to tobacco aromaticss preparation field by the present invention first.Described cave gemmule bacterium
SYSUZL-2 is microorganism novel species, after the bacterial strain and butterflybush flower co-fermentation compared with untreated control, cigarette sweet sense increase,
Flue gas conglobation property improves, the rich increase of fragrance.
(2) cost of the present invention is cheap, simple to operate, is easy to implement large-scale industrial production, and application prospect is good.
The explanation of preservation biomaterial
The bacterial strain of the present invention, on December 9th, 2016, China typical culture collection center (CCTCC) is preserved in,
The centre address：Chinese Wuhan Wuhan Universitys, the strain classification are named as cave gemmule bacterium SYSUZL-2
(Blastococcus cavernae sp.SYSUZL-2), deposit number CCTCC NO：M 2016739.
Embodiment
Below by embodiment, the present invention is described in further detail, but embodiment is not to the technology of the present invention
The restriction of scheme.
Embodiment 1
1st, cave gemmule bacterium SYSUZL-2 strain culturings
(1) inclined-plane culture：Culture medium：Glucose 0.5g；Yeast extract 0.5g；Peptone 0.5g；Acid hydrolyzed casein
0.5g；Soluble starch 0.5g；Sodium Pyruvate 0.3g；Dipotassium hydrogen phosphate 0.3g；Magnesium sulfate 0.05g；Agar 15g；Distilled water
1000ml, pH7.2；Cave gemmule bacterium SYSUZL-2 is taken to be inoculated in slant medium, cultivation temperature is 28 DEG C, incubation time
For 7d.
(2) seed culture：Accessed from inclined-plane picking part mycelium in seed liquor, Shaking culture 36 hours, cultivation temperature
For 28 DEG C, activation bacterium solution is obtained.Seed culture medium：Dextrin 120g；Soy meal 40g；Yeast extract 2g；Tryptophan 0.5g；Beta-alanine
5g；Magnesium sulfate 0.5g；Ammonium phosphate 0.2g；Distilled water 1000ml, pH7.2.
2nd, cave gemmule bacterium SYSUZL-2 strain fermentations prepare tobacco aromaticss
The water of double weight is added after butterflybush flower is crushed, is well mixed, inoculation cave gemmule bacterium activation bacterium solution, vibration
Culture is fermented, and inoculum concentration is the 15% of butterflybush flower weight, and fermentation temperature is 30 DEG C, fermentation time 36h.
3rd, spices extracts
Above-mentioned fermentation liquor backflow is extracted into 3h, filter cleaner, takes supernatant, 50 DEG C are concentrated under reduced pressure into medicinal extract shape, produce institute
Need tobacco aromaticss.To prevent from going bad, by 5% addition propane diols of fermentation spices weight.
4th, product feature and physicochemical property
Gained fermentation tobacco aromaticss are bronzing liquid, are not clarified.Product physicochemical property is shown in Table 1.
The physical and chemical index of the cave gemmule bacterium SYSUZL-2 of table 1 fermentation spices
5th, perfuming cigarette is tested
Fermentation tobacco aromaticss 0.1g is taken, after 50 times of dilution, is uniformly sprayed in the non-perfuming leaf groups of 50g, is loaded clean close
In envelope, after placing 2h at room temperature, pipe tobacco is dried in 120 DEG C of baking ovens, moisture is reached 11%-13%.Will place
Pipe tobacco and control after reason are put into climatic chamber, are balanced 24h under the conditions of 22 DEG C of temperature, humidity 60%, are rolled into by hand
Cigarette, the group of smokeing panel test being made up of the expert that smokes panel test carry out sensory evaluating smoking.With Flos Buddlejae extract and the bacillus subtilis of not fermenting
Butterflybush flower spices ferment for control.Perfuming result shows, butterflybush flower cave gemmule bacterium SYSUZL-2 fermentation spices and control area
Substantially, the rich increase of fragrance, flue gas conglobation property is good, and sweet tea sense increase, miscellaneous gas is reduced.Evaluation result is shown in Table 2.
The butterflybush flower of table 2 fermentation spices smoking result
Embodiment 2
Repeat to implement 1, there is following difference：Cave gemmule bacterium activation bacterium solution addition is the 5% of butterflybush flower weight, hair
Ferment time 24h.
1st, product feature and physicochemical property
Gained fermentation tobacco aromaticss are bronzing liquid, are not clarified.Product physicochemical property is shown in Table 3.
The physical and chemical index of the cave gemmule bacterium SYSUZL-2 of table 3 fermentation butterflybush flower spices
2nd, perfuming cigarette is tested
Fermentation tobacco aromaticss 0.2g is taken, after 50 times of dilution, is uniformly sprayed in the non-perfuming leaf groups of 50g.
Perfuming result shows that gemmule bacterium SYSUZL-2 fermentation spices in butterflybush flower cave is obvious with control area, and fragrance is rich
Richness, sweet taste is more prominent, and miscellaneous gas is reduced, and pleasant impression is comfortable.Evaluation result is shown in Table 4.
The butterflybush flower of table 4 fermentation spices smoking result
Embodiment 3
Repeat to implement 1, there is following difference：Cave gemmule bacterium activation bacterium solution addition is the 20% of butterflybush flower weight, hair
Ferment time 48h.
1st, product feature and physicochemical property
Gained fermentation tobacco aromaticss are bronzing liquid, are not clarified.Product physicochemical property is shown in Table 5.
The physical and chemical index of the cave gemmule bacterium SYSUZL-2 of table 5 fermentation butterflybush flower spices
2nd, perfuming cigarette is tested
Fermentation tobacco aromaticss 0.3g is taken, after 50 times of dilution, is uniformly sprayed in the non-perfuming leaf groups of 50g.
Perfuming result shows that butterflybush flower cave gemmule bacterium SYSUZL-2 fermentation spices and control area are obvious, and sweet tea sense is bright
Aobvious, conglobation property is good, but slightly miscellaneous gas.Evaluation result is shown in Table 6.
The butterflybush flower of table 6 fermentation spices smoking result
Claims (3)
- A kind of 1. tobacco aromaticss prepared using microbial fermentation butterflybush flower, it is characterised in that：Using butterflybush flower as raw material, with micro- life Thing is fermented, then produces product by extracting aroma component in zymotic fluid；Described microorganism is cave gemmule bacterium (Blastococcus cavernae sp) .SYSUZL-2, is preserved in China typical culture collection center, preservation date 2016 On December 9, in, deposit number CCTCC M2016739.
- 2. the tobacco aromaticss according to claim 1 prepared using microbial fermentation butterflybush flower, it is characterised in that specific logical Following methods are crossed to obtain：(1) inclined-plane culture：Cave gemmule bacterium SYSUZL-2 is inoculated with slant medium, 28 DEG C of cultivation temperature, incubation time 7d； Described slant medium is：Glucose 0.5g, yeast extract 0.5g, peptone 0.5g, acid hydrolyzed casein 0.5g, solubility are formed sediment Powder 0.5g, Sodium Pyruvate 0.3g, dipotassium hydrogen phosphate 0.3g, magnesium sulfate 0.05g, agar 15g and distilled water 1000ml, adjust pH value 7.2；(2) seed culture：Accessed from inclined-plane picking mycelium in seed culture medium, Shaking culture 36 hours, 28 DEG C of cultivation temperature, Bacterium solution must be activated；Described seed culture medium is：Dextrin 120g, soy meal 40g, yeast extract 2g, tryptophan 0.5g, Beta-alanine 5g, magnesium sulfate 0.5g, ammonium phosphate 0.2g and distilled water 1000ml, adjust pH value 7.2；(3) ferment：The water of double weight is added after butterflybush flower is crushed, is well mixed, is connect by the 5%~20% of butterflybush flower weight Kind cave gemmule bacterium activation bacterium solution, shaken cultivation are fermented, 30 DEG C of fermentation temperature, 24~48h of fermentation time；(4) spices extracts：Fermentation liquor backflow extraction 3h obtained by step (3) is taken, filter cleaner, takes supernatant, 50 DEG C are concentrated under reduced pressure Into medicinal extract, required tobacco aromaticss are produced.
- 3. application of the tobacco aromaticss described in claim 1 or 2 in production of cigarettes, it is characterised in that：By gained tobacco aromaticss Equably it is sprayed at after dilution on pipe tobacco, the addition of tobacco aromaticss is the 0.2%~0.6% of pipe tobacco weight.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112064405A (en) * | 2020-09-07 | 2020-12-11 | 云南中烟工业有限责任公司 | Special aroma forming paper prepared from rice dyeing flower and method and application thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107034239A (en) * | 2017-05-10 | 2017-08-11 | 云南中烟工业有限责任公司 | Tobacco aromaticss and its application prepared by a kind of utilization microbial fermentation worm dung tea |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107034239A (en) * | 2017-05-10 | 2017-08-11 | 云南中烟工业有限责任公司 | Tobacco aromaticss and its application prepared by a kind of utilization microbial fermentation worm dung tea |
Non-Patent Citations (2)
| Title |
|---|
| HAN，M.X.等: "Blastococcus sp. strain SYSUZL-2 1 6S ribosomal RNA gene, partial sequence", 《GENBANK DATABASE》 * |
| 陈一 等: "生香酵母发酵无花果制备特色烟用香料的研究", 《湖北农业科学》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112064405A (en) * | 2020-09-07 | 2020-12-11 | 云南中烟工业有限责任公司 | Special aroma forming paper prepared from rice dyeing flower and method and application thereof |
| CN112064405B (en) * | 2020-09-07 | 2022-09-13 | 云南中烟工业有限责任公司 | Special fragrant forming paper prepared from rice dyeing flower and method and application thereof |
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