CN102618475B - Bacillus methylotrophicus and preparation method as well as application of tobacco cimicifugae extract - Google Patents
Bacillus methylotrophicus and preparation method as well as application of tobacco cimicifugae extract Download PDFInfo
- Publication number
- CN102618475B CN102618475B CN 201210114009 CN201210114009A CN102618475B CN 102618475 B CN102618475 B CN 102618475B CN 201210114009 CN201210114009 CN 201210114009 CN 201210114009 A CN201210114009 A CN 201210114009A CN 102618475 B CN102618475 B CN 102618475B
- Authority
- CN
- China
- Prior art keywords
- rattletop
- cigarette
- medicinal extract
- tobacco
- extract
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 239000000284 extract Substances 0.000 title claims abstract description 56
- 241000563903 Bacillus velezensis Species 0.000 title claims abstract description 11
- 238000002360 preparation method Methods 0.000 title claims description 12
- 235000002637 Nicotiana tabacum Nutrition 0.000 title abstract description 24
- 241000208125 Nicotiana Species 0.000 title abstract description 23
- 230000036983 biotransformation Effects 0.000 claims abstract description 9
- 235000009508 confectionery Nutrition 0.000 claims abstract description 8
- 206010013911 Dysgeusia Diseases 0.000 claims abstract description 5
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 57
- 235000019504 cigarettes Nutrition 0.000 claims description 41
- 239000003205 fragrance Substances 0.000 claims description 22
- 238000011218 seed culture Methods 0.000 claims description 20
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 18
- 239000000706 filtrate Substances 0.000 claims description 18
- 239000007788 liquid Substances 0.000 claims description 16
- 239000012531 culture fluid Substances 0.000 claims description 15
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 14
- 238000000855 fermentation Methods 0.000 claims description 14
- 230000004151 fermentation Effects 0.000 claims description 14
- 238000000605 extraction Methods 0.000 claims description 13
- 235000013305 food Nutrition 0.000 claims description 11
- 239000002609 medium Substances 0.000 claims description 10
- UGFAIRIUMAVXCW-UHFFFAOYSA-N Carbon monoxide Chemical compound [O+]#[C-] UGFAIRIUMAVXCW-UHFFFAOYSA-N 0.000 claims description 8
- 239000003546 flue gas Substances 0.000 claims description 8
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 claims description 6
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 6
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 claims description 6
- 239000008103 glucose Substances 0.000 claims description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- 229920001817 Agar Polymers 0.000 claims description 5
- 239000001888 Peptone Substances 0.000 claims description 5
- 108010080698 Peptones Proteins 0.000 claims description 5
- 244000061456 Solanum tuberosum Species 0.000 claims description 5
- 235000002595 Solanum tuberosum Nutrition 0.000 claims description 5
- DPDMMXDBJGCCQC-UHFFFAOYSA-N [Na].[Cl] Chemical compound [Na].[Cl] DPDMMXDBJGCCQC-UHFFFAOYSA-N 0.000 claims description 5
- 239000008272 agar Substances 0.000 claims description 5
- 230000006837 decompression Effects 0.000 claims description 5
- 239000001963 growth medium Substances 0.000 claims description 5
- 238000007654 immersion Methods 0.000 claims description 5
- 238000011177 media preparation Methods 0.000 claims description 5
- 235000019319 peptone Nutrition 0.000 claims description 5
- 239000001965 potato dextrose agar Substances 0.000 claims description 5
- 238000001556 precipitation Methods 0.000 claims description 5
- 238000010992 reflux Methods 0.000 claims description 5
- 238000000967 suction filtration Methods 0.000 claims description 5
- 239000000796 flavoring agent Substances 0.000 abstract description 10
- 239000002304 perfume Substances 0.000 abstract description 10
- 235000019634 flavors Nutrition 0.000 abstract description 9
- 230000000813 microbial effect Effects 0.000 abstract description 7
- 239000002994 raw material Substances 0.000 abstract description 6
- 230000008901 benefit Effects 0.000 abstract description 5
- 238000000034 method Methods 0.000 abstract description 5
- 239000000779 smoke Substances 0.000 abstract description 5
- 230000008569 process Effects 0.000 abstract description 3
- 230000007613 environmental effect Effects 0.000 abstract 1
- 238000004519 manufacturing process Methods 0.000 abstract 1
- 102000004190 Enzymes Human genes 0.000 description 9
- 108090000790 Enzymes Proteins 0.000 description 9
- 239000000126 substance Substances 0.000 description 9
- 235000013599 spices Nutrition 0.000 description 8
- 239000010903 husk Substances 0.000 description 7
- 239000000203 mixture Substances 0.000 description 6
- 230000001954 sterilising effect Effects 0.000 description 6
- 238000004659 sterilization and disinfection Methods 0.000 description 6
- YMWUJEATGCHHMB-UHFFFAOYSA-N Dichloromethane Chemical compound ClCCl YMWUJEATGCHHMB-UHFFFAOYSA-N 0.000 description 5
- 239000002253 acid Substances 0.000 description 5
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 238000002290 gas chromatography-mass spectrometry Methods 0.000 description 5
- PXIKRTCSSLJURC-UHFFFAOYSA-N Dihydroeugenol Chemical compound CCCC1=CC=C(O)C(OC)=C1 PXIKRTCSSLJURC-UHFFFAOYSA-N 0.000 description 4
- SMWDFEZZVXVKRB-UHFFFAOYSA-N Quinoline Chemical compound N1=CC=CC2=CC=CC=C21 SMWDFEZZVXVKRB-UHFFFAOYSA-N 0.000 description 4
- 150000001875 compounds Chemical class 0.000 description 4
- 239000007789 gas Substances 0.000 description 4
- 244000005700 microbiome Species 0.000 description 4
- WJUFSDZVCOTFON-UHFFFAOYSA-N veratraldehyde Chemical compound COC1=CC=C(C=O)C=C1OC WJUFSDZVCOTFON-UHFFFAOYSA-N 0.000 description 4
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 3
- 150000001298 alcohols Chemical class 0.000 description 3
- 229910052799 carbon Inorganic materials 0.000 description 3
- 150000002576 ketones Chemical class 0.000 description 3
- 150000002632 lipids Chemical class 0.000 description 3
- 108020004465 16S ribosomal RNA Proteins 0.000 description 2
- KDCGOANMDULRCW-UHFFFAOYSA-N 7H-purine Chemical compound N1=CNC2=NC=NC2=C1 KDCGOANMDULRCW-UHFFFAOYSA-N 0.000 description 2
- 241001647745 Banksia Species 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- 102000004317 Lyases Human genes 0.000 description 2
- 108090000856 Lyases Proteins 0.000 description 2
- 108091005804 Peptidases Proteins 0.000 description 2
- 239000004365 Protease Substances 0.000 description 2
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 2
- 241000220317 Rosa Species 0.000 description 2
- PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 244000223014 Syzygium aromaticum Species 0.000 description 2
- 235000016639 Syzygium aromaticum Nutrition 0.000 description 2
- 150000007513 acids Chemical class 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 239000000654 additive Substances 0.000 description 2
- 230000000996 additive effect Effects 0.000 description 2
- 150000001299 aldehydes Chemical class 0.000 description 2
- PYMYPHUHKUWMLA-LMVFSUKVSA-N aldehydo-D-ribose Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](O)C=O PYMYPHUHKUWMLA-LMVFSUKVSA-N 0.000 description 2
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 description 2
- 238000009835 boiling Methods 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 230000008878 coupling Effects 0.000 description 2
- 238000010168 coupling process Methods 0.000 description 2
- 238000005859 coupling reaction Methods 0.000 description 2
- 230000002950 deficient Effects 0.000 description 2
- 238000004821 distillation Methods 0.000 description 2
- 238000003912 environmental pollution Methods 0.000 description 2
- 230000002255 enzymatic effect Effects 0.000 description 2
- 230000032050 esterification Effects 0.000 description 2
- 238000005886 esterification reaction Methods 0.000 description 2
- 150000004676 glycans Chemical class 0.000 description 2
- 241000411851 herbal medicine Species 0.000 description 2
- JARKCYVAAOWBJS-UHFFFAOYSA-N hexanal Chemical compound CCCCCC=O JARKCYVAAOWBJS-UHFFFAOYSA-N 0.000 description 2
- 230000007062 hydrolysis Effects 0.000 description 2
- 238000006460 hydrolysis reaction Methods 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 230000004060 metabolic process Effects 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 229930014626 natural product Natural products 0.000 description 2
- 230000003647 oxidation Effects 0.000 description 2
- 238000007254 oxidation reaction Methods 0.000 description 2
- 150000004880 oxines Chemical class 0.000 description 2
- 150000002989 phenols Chemical class 0.000 description 2
- DTUQWGWMVIHBKE-UHFFFAOYSA-N phenylacetaldehyde Chemical compound O=CCC1=CC=CC=C1 DTUQWGWMVIHBKE-UHFFFAOYSA-N 0.000 description 2
- 238000006116 polymerization reaction Methods 0.000 description 2
- 229920001282 polysaccharide Polymers 0.000 description 2
- 239000005017 polysaccharide Substances 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 235000019633 pungent taste Nutrition 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 238000006722 reduction reaction Methods 0.000 description 2
- 235000019640 taste Nutrition 0.000 description 2
- BAYUSCHCCGXLAY-UHFFFAOYSA-N 1-(3-methoxyphenyl)ethanone Chemical compound COC1=CC=CC(C(C)=O)=C1 BAYUSCHCCGXLAY-UHFFFAOYSA-N 0.000 description 1
- KBPLFHHGFOOTCA-UHFFFAOYSA-N 1-Octanol Chemical compound CCCCCCCCO KBPLFHHGFOOTCA-UHFFFAOYSA-N 0.000 description 1
- HVGZQCSMLUDISR-UHFFFAOYSA-N 2-Phenylethyl propanoate Chemical compound CCC(=O)OCCC1=CC=CC=C1 HVGZQCSMLUDISR-UHFFFAOYSA-N 0.000 description 1
- ZMAYRLMREZOVLE-UHFFFAOYSA-N 2-ethenyl-6-methoxyphenol Chemical compound COC1=CC=CC(C=C)=C1O ZMAYRLMREZOVLE-UHFFFAOYSA-N 0.000 description 1
- MIDXCONKKJTLDX-UHFFFAOYSA-N 3,5-dimethylcyclopentane-1,2-dione Chemical compound CC1CC(C)C(=O)C1=O MIDXCONKKJTLDX-UHFFFAOYSA-N 0.000 description 1
- UWAOHFMOWBQIJH-UHFFFAOYSA-N 3-cyclopentylpropanal Chemical compound O=CCCC1CCCC1 UWAOHFMOWBQIJH-UHFFFAOYSA-N 0.000 description 1
- AZVSIHIBYRHSLB-UHFFFAOYSA-N 3-furaldehyde Chemical compound O=CC=1C=COC=1 AZVSIHIBYRHSLB-UHFFFAOYSA-N 0.000 description 1
- 235000017060 Arachis glabrata Nutrition 0.000 description 1
- 244000105624 Arachis hypogaea Species 0.000 description 1
- 235000010777 Arachis hypogaea Nutrition 0.000 description 1
- 235000018262 Arachis monticola Nutrition 0.000 description 1
- 108010082340 Arginine deiminase Proteins 0.000 description 1
- 235000002566 Capsicum Nutrition 0.000 description 1
- 240000008574 Capsicum frutescens Species 0.000 description 1
- 244000223760 Cinnamomum zeylanicum Species 0.000 description 1
- KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 description 1
- 235000009917 Crataegus X brevipes Nutrition 0.000 description 1
- 235000013204 Crataegus X haemacarpa Nutrition 0.000 description 1
- 235000009685 Crataegus X maligna Nutrition 0.000 description 1
- 235000009444 Crataegus X rubrocarnea Nutrition 0.000 description 1
- 235000009486 Crataegus bullatus Nutrition 0.000 description 1
- 235000017181 Crataegus chrysocarpa Nutrition 0.000 description 1
- 235000009682 Crataegus limnophila Nutrition 0.000 description 1
- 235000004423 Crataegus monogyna Nutrition 0.000 description 1
- 240000000171 Crataegus monogyna Species 0.000 description 1
- 235000002313 Crataegus paludosa Nutrition 0.000 description 1
- 235000009840 Crataegus x incaedua Nutrition 0.000 description 1
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 description 1
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 1
- FBPFZTCFMRRESA-JGWLITMVSA-N D-glucitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-JGWLITMVSA-N 0.000 description 1
- SRBFZHDQGSBBOR-SOOFDHNKSA-N D-ribopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@@H]1O SRBFZHDQGSBBOR-SOOFDHNKSA-N 0.000 description 1
- LVGKNOAMLMIIKO-UHFFFAOYSA-N Elaidinsaeure-aethylester Natural products CCCCCCCCC=CCCCCCCCC(=O)OCC LVGKNOAMLMIIKO-UHFFFAOYSA-N 0.000 description 1
- 241000167556 Eryngium Species 0.000 description 1
- 229920002527 Glycogen Polymers 0.000 description 1
- 244000215562 Heliotropium arborescens Species 0.000 description 1
- SQUHHTBVTRBESD-UHFFFAOYSA-N Hexa-Ac-myo-Inositol Natural products CC(=O)OC1C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C1OC(C)=O SQUHHTBVTRBESD-UHFFFAOYSA-N 0.000 description 1
- 241001482471 Hosta plantaginea Species 0.000 description 1
- 241001633663 Iris pseudacorus Species 0.000 description 1
- 244000032014 Java citronella Species 0.000 description 1
- ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 description 1
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 description 1
- HNDVDQJCIGZPNO-YFKPBYRVSA-N L-histidine Chemical compound OC(=O)[C@@H](N)CC1=CN=CN1 HNDVDQJCIGZPNO-YFKPBYRVSA-N 0.000 description 1
- 229930195725 Mannitol Natural products 0.000 description 1
- OVRNDRQMDRJTHS-UHFFFAOYSA-N N-acelyl-D-glucosamine Natural products CC(=O)NC1C(O)OC(CO)C(O)C1O OVRNDRQMDRJTHS-UHFFFAOYSA-N 0.000 description 1
- OVRNDRQMDRJTHS-FMDGEEDCSA-N N-acetyl-beta-D-glucosamine Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O OVRNDRQMDRJTHS-FMDGEEDCSA-N 0.000 description 1
- 244000061176 Nicotiana tabacum Species 0.000 description 1
- 235000010676 Ocimum basilicum Nutrition 0.000 description 1
- 240000007926 Ocimum gratissimum Species 0.000 description 1
- 102000052812 Ornithine decarboxylases Human genes 0.000 description 1
- 108700005126 Ornithine decarboxylases Proteins 0.000 description 1
- 241000218201 Ranunculaceae Species 0.000 description 1
- 240000004980 Rheum officinale Species 0.000 description 1
- 235000008081 Rheum officinale Nutrition 0.000 description 1
- NGFMICBWJRZIBI-JZRPKSSGSA-N Salicin Natural products O([C@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@H](CO)O1)c1c(CO)cccc1 NGFMICBWJRZIBI-JZRPKSSGSA-N 0.000 description 1
- 241000581682 Sanguisorba Species 0.000 description 1
- 235000021355 Stearic acid Nutrition 0.000 description 1
- PPBRXRYQALVLMV-UHFFFAOYSA-N Styrene Chemical compound C=CC1=CC=CC=C1 PPBRXRYQALVLMV-UHFFFAOYSA-N 0.000 description 1
- 229920006330 Styrox Polymers 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- 244000290333 Vanilla fragrans Species 0.000 description 1
- 235000009499 Vanilla fragrans Nutrition 0.000 description 1
- 235000012036 Vanilla tahitensis Nutrition 0.000 description 1
- 239000002154 agricultural waste Substances 0.000 description 1
- 235000004279 alanine Nutrition 0.000 description 1
- 150000001335 aliphatic alkanes Chemical class 0.000 description 1
- NGFMICBWJRZIBI-UHFFFAOYSA-N alpha-salicin Natural products OC1C(O)C(O)C(CO)OC1OC1=CC=CC=C1CO NGFMICBWJRZIBI-UHFFFAOYSA-N 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 description 1
- 125000003118 aryl group Chemical group 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- GUBGYTABKSRVRQ-QUYVBRFLSA-N beta-maltose Chemical compound OC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@@H]1O GUBGYTABKSRVRQ-QUYVBRFLSA-N 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 230000003570 biosynthesizing effect Effects 0.000 description 1
- 235000019636 bitter flavor Nutrition 0.000 description 1
- 239000001390 capsicum minimum Substances 0.000 description 1
- 235000013736 caramel Nutrition 0.000 description 1
- 239000012159 carrier gas Substances 0.000 description 1
- 235000019219 chocolate Nutrition 0.000 description 1
- 235000017803 cinnamon Nutrition 0.000 description 1
- 239000010632 citronella oil Substances 0.000 description 1
- 238000010835 comparative analysis Methods 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 150000002148 esters Chemical class 0.000 description 1
- LVGKNOAMLMIIKO-QXMHVHEDSA-N ethyl oleate Chemical compound CCCCCCCC\C=C/CCCCCCCC(=O)OCC LVGKNOAMLMIIKO-QXMHVHEDSA-N 0.000 description 1
- 229940093471 ethyl oleate Drugs 0.000 description 1
- 238000012262 fermentative production Methods 0.000 description 1
- 235000013355 food flavoring agent Nutrition 0.000 description 1
- 235000019990 fruit wine Nutrition 0.000 description 1
- 229940096919 glycogen Drugs 0.000 description 1
- 239000004519 grease Substances 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 description 1
- 150000002475 indoles Chemical class 0.000 description 1
- 239000002440 industrial waste Substances 0.000 description 1
- CDAISMWEOUEBRE-GPIVLXJGSA-N inositol Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@H](O)[C@@H]1O CDAISMWEOUEBRE-GPIVLXJGSA-N 0.000 description 1
- 229960000367 inositol Drugs 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical class CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 1
- 229960002160 maltose Drugs 0.000 description 1
- 239000000594 mannitol Substances 0.000 description 1
- 235000010355 mannitol Nutrition 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- DLRVVLDZNNYCBX-ABXHMFFYSA-N melibiose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1OC[C@@H]1[C@@H](O)[C@H](O)[C@@H](O)C(O)O1 DLRVVLDZNNYCBX-ABXHMFFYSA-N 0.000 description 1
- 150000004702 methyl esters Chemical class 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 229950006780 n-acetylglucosamine Drugs 0.000 description 1
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 description 1
- YOMSJEATGXXYPX-UHFFFAOYSA-N o-methoxy-p-vinylphenol Natural products COC1=CC(C=C)=CC=C1O YOMSJEATGXXYPX-UHFFFAOYSA-N 0.000 description 1
- QIQXTHQIDYTFRH-UHFFFAOYSA-N octadecanoic acid Chemical compound CCCCCCCCCCCCCCCCCC(O)=O QIQXTHQIDYTFRH-UHFFFAOYSA-N 0.000 description 1
- OQCDKBAXFALNLD-UHFFFAOYSA-N octadecanoic acid Natural products CCCCCCCC(C)CCCCCCCCC(O)=O OQCDKBAXFALNLD-UHFFFAOYSA-N 0.000 description 1
- 235000020232 peanut Nutrition 0.000 description 1
- 239000000419 plant extract Substances 0.000 description 1
- 150000003222 pyridines Chemical class 0.000 description 1
- NGFMICBWJRZIBI-UJPOAAIJSA-N salicin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1=CC=CC=C1CO NGFMICBWJRZIBI-UJPOAAIJSA-N 0.000 description 1
- 229940120668 salicin Drugs 0.000 description 1
- 238000009738 saturating Methods 0.000 description 1
- CDAISMWEOUEBRE-UHFFFAOYSA-N scyllo-inosotol Natural products OC1C(O)C(O)C(O)C(O)C1O CDAISMWEOUEBRE-UHFFFAOYSA-N 0.000 description 1
- 238000002864 sequence alignment Methods 0.000 description 1
- 230000000391 smoking effect Effects 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 229910052938 sodium sulfate Inorganic materials 0.000 description 1
- 235000011152 sodium sulphate Nutrition 0.000 description 1
- 235000010356 sorbitol Nutrition 0.000 description 1
- 229960002920 sorbitol Drugs 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 239000008117 stearic acid Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 229960004793 sucrose Drugs 0.000 description 1
- 235000019605 sweet taste sensations Nutrition 0.000 description 1
- 150000003505 terpenes Chemical class 0.000 description 1
- 235000007586 terpenes Nutrition 0.000 description 1
- 235000019505 tobacco product Nutrition 0.000 description 1
Abstract
The invention discloses bacillus methylotrophicus, wherein the name of the strain is bacillus methylotrophicus VJ4-1 with a collection number of CCTCC NO: M 2012004. The invention provides biotransformation of tobacco rhizoma cimicifugae extract by using microbial bacillus methylotrophicus and application of the tobacco rhizoma cimicifugae extract to tobacco. Natural tobacco flavor can be produced by fermenting rhizoma cimicifugae through microbial strain. The method has the advantages of simple process, wide source of raw materials, environmental friendliness and harmlessness in a production process and industrial application prospect; and the obtained natural flavor extract can be directly used for flavoring of tobacco, has the advantages of coordination with smoke, light sweet flowery flavor, rich aroma, softness, comfort, good aftertaste and capability of improving smoke flavor and can be mixed with other tobacco essence and perfumes for use.
Description
Technical field
The present invention relates to the tobacco aromatics using technical field, specifically refer to a kind of preparation method and application that eat methyl alcohol genus bacillus and cigarette usefulness rattletop medicinal extract thereof.
Background technology
In tobacco product, the fragrance of tobacco and suction flavor are most important, and interpolation spices is the key link in the cigarette process, also are the effective means of improving product style and improving the cigarette flavor quality.Tobacco aromatics using has dividing of chemically composite perfume and natural perfume.In recent years, natural plant pefume, natural harmless because it is with Chinese characteristics, the raw material reason such as be easy to get, composition Research Emphasis and focus.The exploitation of plant extract spices is owing to the limited limitation that exists of available plant resources, microbial fermentation engineering is applied in the biosynthesizing of essence and flavoring agent, take industrial and agricultural waste as raw material, utilize microorganism can produce various natural perfumes, be a kind of good solution route.
Rattletop, Latin is called Rhizoma cimicifugae, a kind of ranunculaceae plant herbal medicine.Be irregular microscler bulk, multi-branched is nodositas, long 10 ~ 20cm, diameter 2 ~ 4cm.Surface chocolate or brown, coarse injustice has hard thin fibrous root residual, and the above has the stem foot trace in several circular cavities, and the hole inwall shows netted rill; Below uneven, the tool mark of fibrous root.Body is light, and matter is hard, frangibility not, and section is uneven, the crack is arranged, fibering, yellow-green colour or yellowish white.Gas is little, mildly bitter flavor and puckery.Rattletop one medicine mainly contains saturating the sending out of elevate a turnable ladder and reaches the effects such as clearing heat and detoxicating.Rhizoma Cimicifugae extract medicinal extract adds the significant deficiency that existence is inharmonious with flue gas in the cigarette, pungency large, assorted gas weighs to, can't cigarette usefulness.
Microorganism can produce huge high active enzyme system in breeding, such as the polysaccharide hydrolysis enzyme, protease, the esterification enzyme, redox enzymes and lyases etc., in enzymatic action, under the synergy of the interior complicated metabolism of chemical action and microbe, decompose take rattletop as raw material, degraded, oxidation, reduction, polymerization, coupling, the effects such as conversion, form complicated low molecular compound, comprising various perfume compounds, such as alcohols, aldehydes, ketone, acids, lipid, phenols, the pyrans class, pyridines and terpenes etc., these aroma substances also are tobacco or the aroma component that is used for the additive of tobacco undoubtedly.
Chemosynthesis tobacco extract extraction step is complicated at present, and environmental pollution is serious, and the fragrance taste of tobacco extract is single and aroma strength is still inadequate.Rattletop and fermented product medicinal extract thereof carry out cigarette with yet there are no report.
Summary of the invention
Complicated for present chemosynthesis tobacco extract extraction step, environmental pollution is serious, single and the aroma strength of the fragrance taste of tobacco extract is inadequate defective still, the invention provides a kind of food methyl alcohol genus bacillus VJ4-1, this bacterial strain is called Bacillus methylotrophicus VJ4-1, be preserved in Wuhan University Chinese Typical Representative culture collection center on January 11st, 2012, deposit number CCTCC NO:M 2012004, its biological property is: be gram-positive microorganism, can in 10%NaCl, grow, arginine dihydrolase is arranged, ornithine decarboxylase activity can produce acid, make milk liquefaction; Can grow on the substratum of carbon source take N-Acetyl-D-glucosamine, N.F,USP MANNITOL, glucose, salicin, D-melibiose, D-ribose, inositol, sucrose, maltose, D-glucitol, L-arabinose, DL-LACTIC ACID salt, ALANINE glycogen, L-PROLINE Histidine, Citrate trianion, the 16S rRNA sequence alignment similarity of its 16S rRNA sequence and Bacillus methylotrophicus reaches 100%.
The invention provides a kind of preparation method who utilizes food methyl alcohol genus bacillus VJ4-1Bacillus methylotrophicus VJ4-1 CCTCC NO:M 2012004 bio-transformation cigarettes to use husk medicinal extract, may further comprise the steps:
1) rattletop is crushed to 10 ~ 200 orders, filters after 1 ~ 3 hour with water 1: 20 in mass ratio ~ 40 refluxing extraction, filtrate is sterilized;
2) slant culture: will eat methyl alcohol genus bacillus VJ4-1Bacillus methylotrophicus VJ4-1 CCTCC NO:M 2012004 and be inoculated on the potato dextrose agar slant medium, under 25 ~ 35 ℃, pH5 ~ 10 conditions, leave standstill to cultivate and be placed in the refrigerator in 24 ~ 72 hours;
3) slant culture of gained is inoculated into seed culture medium preparation seed culture fluid: with above-mentioned steps 2), at 25 ~ 35 ℃, and shaking culture 12 ~ 36h under rotating speed 100 ~ 150rpm condition, transferring continuously makes seed culture fluid after 1 ~ 4 time;
4) form fermented liquid in the rattletop filtrate fermentation: seed culture fluid 5 ~ 20% is inoculated into above-mentioned steps 1 by volume), the tinning amount of above-mentioned fermented liquid is 20 ~ 40%, in pH4 ~ 10, and 25 ~ 40 ℃ of temperature, rotating speed 100 ~ 200rpm, lucifuge condition bottom fermentation is 7 ~ 14 days;
5) with fermented liquid and 95% ethanol 1: 3 by volume ~ 4 of gained in the step 4), the precipitation rear suction filtration that spends the night with the concentrated desolventizing of filtrate decompression, makes relative density and is 1.20 ~ 1.36 cigarette rattletop medicinal extract.
Preferentially, described slant medium is: potato immersion liquid 200mL/L, glucose 20g/L, agar 13g/L.
Preferentially, described seed liquor substratum consists of: maltose 50g/L, peptone 10g/L, sodium-chlor 5g/L.
The invention provides the application of rattletop medicinal extract of a kind of gained cigarette, it is characterized in that: described cigarette adds in the cigarette with rattletop medicinal extract, be used for to coordinate that flue gas, flue gas are coordinated, increase the light sweet fragrance of a flower, enriched fragrance, soft comfortable, good after-taste, improvement inhale flavor, adding cigarette in the cigarette is 0.01 ~ 0.05% with the mass ratio of rattletop medicinal extract.
Adopt GC/MS to utilizing food methyl alcohol genus bacillus VJ4-1Bacillus methylotrophicus VJ4-1 CCTCC NO:M 2012004 bio-transformation cigarettes to concentrate the medicinal extract (hereinafter to be referred as Rhizoma Cimicifugae extract medicinal extract) of making under rattletop extracting solution the same terms that the methyl alcohol genus bacillus experiences identical fermentation step and carry out the volatility aroma component and compare with not utilizing to eat with rattletop medicinal extract.
(1) while distillation extraction
5g rattletop fermented type tobacco extract is packed in the round-bottomed flask, adds 400mL water, be heated to boiling with electric mantle, add the methylene dichloride of 200mL in the round-bottomed flask of opposite side, 60 ℃ of water-baths boilings, until in the middle of separating tube place liquid level layering appears and after timing extraction 2h.Extraction adds anhydrous sodium sulphate after finishing in dichloromethane extraction liquid, spend the night, and filters and be concentrated into 1mL, and concentrated solution is used for carrying out GC/MS and analyzes.Rhizoma Cimicifugae extract medicinal extract carries out the while distillation extraction equally, and its extraction concentrated solution is used for carrying out GC/MS and analyzes.
(2) GC/MS analyzes: HP-5MS (50m * 0.25mm * 0.25 μ m) capillary column; Sample introduction temperature: 250 ℃; Splitting ratio 20:1; Carrier gas: He, 1 mL/min; Heating schedule: be raised to 250 ℃ (5min) with 5 ℃/min from 50 ℃ (1 min); Transmission line temperature: 250 ℃; Ion source: EI source; Electron energy: 70eV; Sweep limit: 50 ~ 550amu; Use WILEY and MINILAB spectrum storehouse to retrieve.
(3) volatile component comparative analysis
By GC/MS cigarette of the present invention is carried out analysis of aroma components with rattletop medicinal extract, as can be seen from Table 1, identify altogether 46 kinds of aroma components, wherein the ester class is 11 kinds, 7 kinds of ketones, 3 kinds of aldehydes, 4 kinds of alcohols, and 21 kinds of aroma substances such as alkane, methyl vanillin, dihydroeugenol, β-different methyl-jononeionone, purine, quinoline, lipid acid.Contrast Rhizoma Cimicifugae extract medicinal extract, cigarette has newly increased 23 kinds of aroma substances with rattletop medicinal extract, has disappeared 8 kinds.The aroma substance that newly increases comprises: hexanal has the peculiar fragrance of fruit sample during lower concentration; The 3-furfural has caramel, belongs to natural equivalent spices; Phenylacetic aldehyde has strong Flower of Fragrant Plantainlily fragrance; The 3-methoxyacetophenone has strong hawthorn fragrance; Dihydro cinnamon acid methyl esters is strong fruit wine perfume and sweet fragrance of a flower gas, and natural product are present in capsicum, rheum officinale and the sweet basil etc.; Dihydroeugenol, 1% solution has fragrant and sweet, Xin Xiang, cloves, blue or green perfume (or spice) and fragrance of a flower fragrance, and with grease and banksia rose note, belongs to natural equivalent spices; Methyl vanillin is the fragrant and sweet banksia rose and Heliotropium arborescens fragrance, dense vanilla is arranged like sweet taste, and natural product are present in Java citronella oil and the Eryngium poterium wet goods; β-different methyl-jononeionone has soft violet fragrance, naturally is present in yellow flag.The vicissitudinous aroma substance of percentage composition comprises relatively: vinylbenzene, special aroma is arranged, and be present in the Styrox (a kind of natural perfume); N-Octanol has strong aromatic odour; To vinyl guaiacol, be strong spice, cloves and fermentation like fragrance, the breath of the peanut of stir-fry is arranged; Stearic acid has faint special aroma; Ethyl oleate is fresh flower flavor.All these fragrance blend into one and give flue gas coordination, the light sweet fragrance of a flower, enrich fragrance, soft comfortable, good after-taste.
Microorganism can produce huge high active enzyme system in breeding, such as the polysaccharide hydrolysis enzyme, protease, the esterification enzyme, redox enzymes and lyases etc., in enzymatic action, under the synergy of the interior complicated metabolism of chemical action and microbe, decompose take rattletop as raw material, degraded, oxidation, reduction, polymerization, coupling, the effects such as conversion, form complicated low molecular compound, comprising various perfume compounds, such as alcohols, aldehydes, ketone, acids, lipid, phenols, the pyrans class, indoles and quinoline etc., these aroma substances also are tobacco or the aroma component that is used for the additive of tobacco undoubtedly.
Table 1. cigarette contrasts with the volatile aroma composition of rattletop medicinal extract and contrast-Rhizoma Cimicifugae extract medicinal extract
Remarks: phenylethyl propionate is interior mark, is 5.467 with cigarette with the relative percentage composition ratio in the rattletop medicinal extract aroma component at contrast-Rhizoma Cimicifugae extract medicinal extract according to it: 5.123=1.067: 1, come the variation of the relative percentage composition of both aroma substances of comparison.
Advantage of the present invention:
1, the present invention take the herbal medicine rattletop as sole carbon source, nitrogenous source and substrate, elevate a turnable ladder is thoroughly sent out and is clearing heat and detoxicating etc.
Effect, more easily allowing the microbial enzyme system be used to fermentation than single carbon source and produce perfume, wide material sources have the harmless advantage of natural environmental-protective.
2, adopt food methyl alcohol bacillus methylotrophicus VJ4-1 CCTCC NO:M 2012004 come fermentative production with flue gas coordinations, the light sweet fragrance of a flower, enrich the cigarette rattletop medicinal extract that fragrance, soft comfortable, good after-taste, improvement are inhaled flavor, can with other essence spice for cigarette mixing preparation use.
3, the inventive method technological process is simple, and raw material sources extensively are easy to get, and the industrialization prospect of the application is arranged.
Embodiment
The below enumerates a part of embodiment Relational Questions of the present invention is described in further detail, and is necessary to point out that at this following specific embodiment just further specifies of the present invention, does not represent limiting the scope of the invention.Some nonessential modification and adjustment that other people make according to the present invention still belong to protection scope of the present invention.
Embodiment 1
The present invention utilizes microbial food methyl alcohol genus bacillus VJ4-1Bacillus methylotrophicus VJ4-1 CCTCC NO:M 2012004 bio-transformation cigarettes as follows with the preparation method of husk medicinal extract:
1) rattletop is crushed to 10 orders, refluxing extraction 3h after filters at 1: 20 in mass ratio with water, with filtrate 115 ℃ of high-temperature sterilizations 25 minutes;
2) slant culture: will eat methyl alcohol genus bacillus VJ4-1Bacillus methylotrophicus VJ4-1 CCTCC NO:M 2012004 and be inoculated on the potato dextrose agar slant medium, under 25 ℃, pH 10 conditions, leave standstill and cultivate 24h and be placed in the refrigerator;
Slant medium is: potato immersion liquid 200mL/L, glucose 20g/L, agar 13g/L;
3) slant culture of gained is inoculated into seed culture medium preparation seed culture fluid: with above-mentioned steps 2), at 25 ℃, and shaking culture 12h under the rotating speed 150rpm condition, transferring continuously makes seed culture fluid after 4 times;
The seed liquor substratum consists of: maltose 50g/L, peptone 10g/L, sodium-chlor 5g/L;
The filtrate of the sterilization 4) fermentation: seed culture fluid 5% is inoculated into above-mentioned steps 1 by volume), the tinning amount of postvaccinal filtrate is 40%, at pH 4,40 ℃ of temperature, rotating speed 100rpm, lucifuge condition bottom fermentation is 14 days;
5) with the fermented liquid of gained in the step 4) and 95% ethanol 1: 4 by volume, the precipitation rear suction filtration that spends the night with the concentrated desolventizing of filtrate decompression, makes relative density and is 1.20 cigarette husk medicinal extract.
Embodiment 2
The microbial food methyl alcohol genus bacillus VJ4-1 Bacillus methylotrophicus VJ4-1 CCTCC NO:M 2012004 bio-transformation cigarettes that utilize of the present invention are as follows with the preparation method of husk medicinal extract:
1) rattletop is crushed to 100 orders, refluxing extraction 2h after filters at 1: 30 in mass ratio with water, with filtrate 115 ℃ of high-temperature sterilizations 25 minutes;
2) slant culture: will eat methyl alcohol genus bacillus VJ4-1 Bacillus methylotrophicus VJ4-1 and be inoculated on the potato dextrose agar slant medium, and under 30 ℃, pH 7 conditions, leave standstill and cultivate 36h and be placed in the refrigerator;
Slant medium is: potato immersion liquid 200mL/L, glucose 20g/L, agar 13g/L;
3) slant culture of gained is inoculated into seed culture medium preparation seed culture fluid: with above-mentioned steps 2), at 30 ℃, and shaking culture 24h under the rotating speed 120rpm condition, transferring continuously makes seed culture fluid after 3 times;
Described seed liquor substratum consists of: maltose 50g/L, peptone 10g/L, sodium-chlor 5g/L;
The filtrate of the sterilization 4) fermentation: seed culture fluid 10% is inoculated into above-mentioned steps 1 by volume), the tinning amount of postvaccinal filtrate is 30%, at pH 8,30 ℃ of temperature, rotating speed 150rpm, lucifuge condition bottom fermentation is 10 days;
5) with the fermented liquid of gained in the step 4) and 95% ethanol 1: 3 by volume, the precipitation rear suction filtration that spends the night with the concentrated desolventizing of filtrate decompression, makes relative density and is 1.36 cigarette husk medicinal extract.
Embodiment 3
The present invention utilizes microbial food methyl alcohol genus bacillus VJ4-1Bacillus methylotrophicus VJ4-1 CCTCC NO:M 2012004 bio-transformation cigarettes as follows with the preparation method of husk medicinal extract:
1) rattletop is crushed to 200 orders, refluxing extraction 1h after filters at 1: 40 in mass ratio with water, with filtrate 115 ℃ of high-temperature sterilizations 25 minutes;
2) slant culture: will eat methyl alcohol genus bacillus VJ4-1Bacillus methylotrophicus VJ4-1 CCTCC NO:M 2012004 and be inoculated on the potato dextrose agar slant medium, under 35 ℃, pH 5 conditions, leave standstill and cultivate 72h and be placed in the refrigerator;
Slant medium is: potato immersion liquid 200mL/L, glucose 20g/L, agar 13g/L;
3) slant culture of gained is inoculated into seed culture medium preparation seed culture fluid: with above-mentioned steps 2), at 35 ℃, and shaking culture 36h under the rotating speed 100rpm condition, transferring continuously makes seed culture fluid after 2 times;
Described seed liquor substratum consists of: maltose 50g/L, peptone 10g/L, sodium-chlor 5g/L
The filtrate of the sterilization 4) fermentation: seed culture fluid 20% is inoculated into above-mentioned steps 1 by volume), the tinning amount of postvaccinal filtrate is 40%, at pH10,25 ℃ of temperature, rotating speed 200rpm, lucifuge condition bottom fermentation is 7 days;
5) with the fermented liquid of gained in the step 4) and 95% ethanol 1: 3 by volume, the precipitation rear suction filtration that spends the night with the concentrated desolventizing of filtrate decompression, makes relative density and is 1.28 cigarette husk medicinal extract.
Embodiment 4
The contriver with above-mentioned cigarette with rattletop medicinal extract in pipe tobacco amount 0.01%, 0.02%, 0.05% ratio, Rhizoma Cimicifugae extract medicinal extract in pipe tobacco amount ratio 0.01%, add in the pipe tobacco respectively and smoke panel test, smoke panel test and carry out according to GB GB 5606.4-1996.Smoking result is as shown in table 2 below.Repeatedly smoke panel test through the expert group of smokeing panel test, think: this rattletop fermented type tobacco extract, add to when ratio is 0.01%, 0.02%, 0.05% in the cigarette, have with flue gas and coordinate, enrich the advantage of fragrance, lifting aroma quality, can use with other essence spice for cigarette mixing preparation, improve the defective that Rhizoma Cimicifugae extract medicinal extract is inharmonious in cigarette, pungency large, assorted gas weighs.
The application of table 2. rattletop fermented type tobacco extract in cigarette charging
Claims (4)
1. one kind is utilized food methyl alcohol genus bacillus bio-transformation cigarette with the preparation method of rattletop medicinal extract, it is characterized in that, may further comprise the steps:
1) rattletop is crushed to 10 ~ 200 orders, with water in mass ratio 1:20 ~ 40 refluxing extraction filter after 1 ~ 3 hour, filtrate sterilized;
2) slant culture: will eat methyl alcohol genus bacillus (Bacillus methylotrophicus) VJ4-1 and be inoculated on the potato dextrose agar slant medium, under 25 ~ 35 ℃, pH5 ~ 10 conditions, leave standstill to cultivate and be placed in the refrigerator described food methyl alcohol genus bacillus (Bacillus methylotrophicus) VJ4-1 deposit number CCTCC NO:M 2012004 in 24 ~ 72 hours;
3) slant culture of gained is inoculated into seed culture medium preparation seed culture fluid: with above-mentioned steps 2), at 25 ~ 35 ℃, and shaking culture 12 ~ 36h under rotating speed 100 ~ 150rpm condition, transferring continuously makes seed culture fluid after 1 ~ 4 time;
4) form fermented liquid in the rattletop filtrate fermentation: seed culture fluid 5 ~ 20% is inoculated into above-mentioned steps 1 by volume), the tinning amount of above-mentioned fermented liquid is 20 ~ 40%, in pH4 ~ 10, and 25 ~ 40 ℃ of temperature, rotating speed 100 ~ 200rpm, lucifuge condition bottom fermentation is 7 ~ 14 days;
5) with fermented liquid and 95% ethanol 1: 3 by volume ~ 4 of gained in the step 4), the precipitation rear suction filtration that spends the night with the concentrated desolventizing of filtrate decompression, makes relative density and is 1.20 ~ 1.36 cigarette rattletop medicinal extract.
2. a kind of food methyl alcohol genus bacillus bio-transformation cigarette that utilizes according to claim 1 is characterized in that with the preparation method of rattletop medicinal extract, and described slant medium is: potato immersion liquid 200mL/L, glucose 20g/L, agar 13g/L.
3. a kind of food methyl alcohol genus bacillus bio-transformation cigarette that utilizes according to claim 1 is characterized in that with the preparation method of rattletop medicinal extract, and described seed liquor substratum consists of: maltose 50g/L, peptone 10g/L, sodium-chlor 5g/L.
4. according to claim 1 application of gained cigarette usefulness rattletop medicinal extract, it is characterized in that: described cigarette adds in the cigarette with rattletop medicinal extract, be used for coordinating flue gas, increase the light sweet fragrance of a flower, enrich fragrance, make soft comfortable, the good after-taste of flue gas, cigarette is 0.01 ~ 0.05% with the mass ratio of rattletop medicinal extract in the adding cigarette.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201210114009 CN102618475B (en) | 2012-04-18 | 2012-04-18 | Bacillus methylotrophicus and preparation method as well as application of tobacco cimicifugae extract |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201210114009 CN102618475B (en) | 2012-04-18 | 2012-04-18 | Bacillus methylotrophicus and preparation method as well as application of tobacco cimicifugae extract |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102618475A CN102618475A (en) | 2012-08-01 |
| CN102618475B true CN102618475B (en) | 2013-05-01 |
Family
ID=46558689
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201210114009 Expired - Fee Related CN102618475B (en) | 2012-04-18 | 2012-04-18 | Bacillus methylotrophicus and preparation method as well as application of tobacco cimicifugae extract |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102618475B (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106011175A (en) * | 2016-06-03 | 2016-10-12 | 湖北中烟工业有限责任公司 | Method for biotransformation preparation of natural cocoa spice and application of natural cocoa spice |
| CN106520376A (en) * | 2016-11-30 | 2017-03-22 | 湖北中烟工业有限责任公司 | Preparation method of common fenugreek seed extract for cigarette |
| CN106520377A (en) * | 2016-12-01 | 2017-03-22 | 湖北中烟工业有限责任公司 | Preparation method of fermented tobacco leaf extract capable of reducing irritation and application of fermented tobacco leaf extract to recombinant tobacco leaves |
| CN109055270A (en) * | 2018-08-31 | 2018-12-21 | 广东容大生物股份有限公司 | A kind of high-density cultivation method and culture medium for bacillus |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101423785A (en) * | 2007-10-30 | 2009-05-06 | 武汉烟草(集团)有限公司 | Method for preparing perfume for red jujube fermentation type wine cigarette |
| CN102119784A (en) * | 2011-02-23 | 2011-07-13 | 红云红河烟草(集团)有限责任公司 | Spice extractum prepared by waste tobacco leaf, preparation method thereof and application thereof |
| CN102191130A (en) * | 2011-05-19 | 2011-09-21 | 湖北中烟工业有限责任公司 | Preparation method of fermented cigarette fructus momordicae extractum |
-
2012
- 2012-04-18 CN CN 201210114009 patent/CN102618475B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101423785A (en) * | 2007-10-30 | 2009-05-06 | 武汉烟草(集团)有限公司 | Method for preparing perfume for red jujube fermentation type wine cigarette |
| CN102119784A (en) * | 2011-02-23 | 2011-07-13 | 红云红河烟草(集团)有限责任公司 | Spice extractum prepared by waste tobacco leaf, preparation method thereof and application thereof |
| CN102191130A (en) * | 2011-05-19 | 2011-09-21 | 湖北中烟工业有限责任公司 | Preparation method of fermented cigarette fructus momordicae extractum |
Non-Patent Citations (6)
| Title |
|---|
| G. Maldonado-Robledo等.Production of tobacco aroma from lutein. Specific role of the microorganisms involved in the process.《Applied Microbiology and Biotechnology》.2003,第62卷484-488页. |
| Production of tobacco aroma from lutein. Specific role of the microorganisms involved in the process;G. Maldonado-Robledo等;《Applied Microbiology and Biotechnology》;20030624;第62卷;484-488页 * |
| 利用香荚兰内生菌制备天然香料及其在卷烟中的应用;李雪梅 等;《烟草科技》;20080331(第3期);31-34、62页 * |
| 朱宇 等.西瓜内生菌的挥发性成分分析及其发酵香料在卷烟中的应用.《光谱实验室》.2011,第28卷(第4期),2093-2097页. |
| 李雪梅 等.利用香荚兰内生菌制备天然香料及其在卷烟中的应用.《烟草科技》.2008,(第3期),31-34、62页. |
| 西瓜内生菌的挥发性成分分析及其发酵香料在卷烟中的应用;朱宇 等;《光谱实验室》;20110731;第28卷(第4期);2093-2097页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102618475A (en) | 2012-08-01 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102851228B (en) | Preparation method and application of Bacillus methylotrophicus and tobacco spine date seed extract thereof | |
| CN102978127B (en) | Tobacco leaf extract and cocoa extract composite fermentation perfume preparation method and applications of tobacco leaf extract and cocoa extract composite fermentation perfume in recombinant tobacco leaf | |
| CN102628026B (en) | Bacillus methylotrophicus and preparation method and application of tobacco coffee extract thereof | |
| CN106551422A (en) | Preparation method and application of the cigarette with Semen Ziziphi Spinosae extractum | |
| CN102851241B (en) | Preparation method for irritation reducing fermentation tobacco extract, and applications of irritation reducing fermentation tobacco extract in recombinant tobacco | |
| CN107523518B (en) | Stenotrophomonas maltophilia and application thereof | |
| CN102864103A (en) | Composite fermentation spice including tobacco leaf, trigonella foenum-graecum L., capsicum annuum and solanum lycopersicum and application thereof to recombinant tobacco leaf | |
| CN107227204A (en) | A kind of cichory root tobacco aromaticss and its preparation method and application | |
| CN102618475B (en) | Bacillus methylotrophicus and preparation method as well as application of tobacco cimicifugae extract | |
| CN102851230B (en) | Preparation method and application of Bacillus methylotrophicus and tobacco chaff extract thereof | |
| CN102851229B (en) | Preparation method and application of Bacillus methylotrophicus and tobacco angelica sinensis extract thereof | |
| CN106434828A (en) | Preparation method of fermented parochetus communis perfumes for cigarettes | |
| CN107034239A (en) | Tobacco aromaticss and its application prepared by a kind of utilization microbial fermentation worm dung tea | |
| CN106929536A (en) | A kind of bio perfume (or spice) prepares method and its application of Momordica grosvenori spices | |
| CN103224894B (en) | Enterobacter mori, and biotransformation method for fermented wheat bran extract used for cigarette and application | |
| CN109090697B (en) | Method for fermenting tobacco by mixed bacteria | |
| CN107043788A (en) | A kind of method and its application that fig flavor is prepared based on bio perfume (or spice) | |
| CN102732381A (en) | Preparation method of fermented tobacco Dendranthema indicum essential oil | |
| CN105713847B (en) | A kind of trichoderma aureoviride bacterial strain and the method for preparing sanchi flower tobacco aromaticss with it | |
| CN108220344A (en) | A kind of tobacco aromaticss prepared using microbial fermentation Stauntonia latifolia and its application | |
| CN108142988B (en) | Preparation method of momordica grosvenori extract for increasing aroma and keeping moisture | |
| CN105713924A (en) | Method for preparing fermented type tobacco spice from rosa sterilis | |
| CN106520377A (en) | Preparation method of fermented tobacco leaf extract capable of reducing irritation and application of fermented tobacco leaf extract to recombinant tobacco leaves | |
| CN106753794A (en) | A kind of fermentation type tobacco preparation method of Dendranthema indicum essential oil | |
| CN102392050B (en) | Endophyte bio-transformed raisin extract and preparation and application in flavoring thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130501 Termination date: 20140418 |