CN108175122A - A kind of method that quality of tobacco is promoted using molten bacillus - Google Patents
A kind of method that quality of tobacco is promoted using molten bacillus Download PDFInfo
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- CN108175122A CN108175122A CN201711452343.1A CN201711452343A CN108175122A CN 108175122 A CN108175122 A CN 108175122A CN 201711452343 A CN201711452343 A CN 201711452343A CN 108175122 A CN108175122 A CN 108175122A
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- tobacco
- quality
- molten bacillus
- microbial inoculum
- tobacco leaf
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- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
- A24B15/18—Treatment of tobacco products or tobacco substitutes
- A24B15/20—Biochemical treatment
Abstract
The invention discloses a kind of methods that quality of tobacco is promoted using molten bacillus.The strain classification is named as molten bacillus (Lysobacter sp) C8 1, and China Committee for Culture Collection of Microorganisms's common micro-organisms center, deposit number CGMCC No.1.16271 are preserved within 28th in September in 2017.Show that the bacterial strain is a kind of new bacterium by Microbiological Characteristics analysis and 16S rDNA gene sequencings.Tobacco leaf spray 1 microbial inoculums of C8 again through alcoholization ferment after, quality of tobacco can be improved, greatly improve tobacco leaf usability, accelerating fermentation of leaf tobacco by use improves tobacco leaf degree quality, increases quality of tabacco fragrance, content of reducing sugar, decline protein, cellulose, total nitrogen, nicotine content, sugared nitrogen, than the ratio rising with nitrogen base ratio, makes tobacco leaf intrinsic chemical ingredient more coordinate, organoleptic quality is also obviously improved than, sugared alkali.
Description
Technical field
The invention belongs to alcoholized tobacco fermentation technical fields, are specifically related to a kind of newfound molten bacillus of utilization and promote cigarette
Method in leaf quality.
Background technology
Tobacco is one of mainstay of China's finance, is made a great contribution to the social and economic development in China.It will
China's tobacco process is promoted, realizes cigarette industry sustainable development, must just walk the road of tobacco scientific and technical innovation.The quality of tobacco leaf is straight
Connect the inherent suction taste for affecting cigarette.State Tobacco Monopoly Bureau explicitly points out, " capturing influences tobacco aroma quality, perfume quantity
Key technology ", diminution and external quality of tobacco gap, to improve domestic and international market competitiveness.
Tobacco fermentation is the main technique link in tobacco processing course.In fermentation process is refined, tobacco leaf primary chemical
Corresponding variation occurs into branch, blue foreign smell and irritation continuously decrease, and fragrance appears, jealous alcohol and, uniform color is simultaneously deepened,
So tobacco mellowing fermentation plays very important effect to the change of quality of tobacco.Tobacco fermentation technology is improved, promotes tobacco leaf
Flavor and taste quality, it has also become cigarette industry improve technology level important means.
Microbe species enrich, and for a long time, people carry out the microorganism in environment investigation using pure culture technigne and grind
Study carefully and develop and use.With the development of science and technology, importance of the microbial resources in Technological research and biological industry is increasingly
It highlights.Many countries are all collecting, are preserving various microbial resources in the world, enrich constantly the hair for China's biotechnology
Solid material base is established in exhibition.Domestic and international tobacco worker detaches in tobacco microorganism and improves tobacco using microbial fermentation
Quality, improvement tobacco fermentation process, shortening tobacco fermentation period etc. have done many work, especially by application selection and breeding
Microorganism and preparation reduce the harmful components such as nicotine in tobacco, by the metabolic activity of microorganism, increase tobacco flavor and taste, carry
High tobacco quality etc..But involved microorganism is limited to aroma-producing yeast more.
The present invention is identified as microorganism novel species, does not have at present for the first time from a kind of isolated molten bacillus strain of nature
Any open report about the molten bacillus and application thereof.
Invention content
In view of the above-mentioned deficiencies in the prior art, it is an object of the present invention to provide a kind of side that quality of tobacco is promoted using molten bacillus
Method.
The purpose of the present invention is achieved by the following technical programs.
Unless otherwise indicated, percentage of the present invention is mass percent.
A kind of method that quality of tobacco is promoted using molten bacillus, it is characterised in that：Molten bacillus is made after microbial inoculum and tobacco leaf
Co-fermentation；The molten bacillus Classification And Nomenclature is molten bacillus (Lysobacter sp.) C8-1, and the bacterial strain was in September 28 in 2017
Day is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center CGMCC, deposit number CGMCC
No.1.16271。
The co-fermentation is specially：Microbial inoculum is made after molten bacillus C8-1 is expanded fermented and cultured, weighs pre-balance
The pipe tobacco of moisture to 13% sprays molten bacillus microbial inoculum, in terms of pipe tobacco weight, every 1000 grams of pipe tobaccos spray microbial inoculum amount for 10~
30mL, then by treated, pipe tobacco is placed in 22 DEG C, and ferment 48h in the constant-temperature constant-humidity environment of humidity 60%, 120 DEG C of drying cigarettes
Silk.
Molten bacillus (Lysobacter sp) C8-1 of the present invention, is screened to obtain by following methods：
Isolated, the solid culture process from the tobacco rhizosphere soil for being collected in Yunnan Province Kunming tobacco planting ground
In, finding the microorganism of the overwhelming majority has the ability for generating volatile materials, and the breath generated after liquid fermentation is significantly not
Together.In the isolation of pure culture bacterial strain of acquisition, screening obtain can generating on the culture medium containing simple carbon nitrogen source volatility and
Bacterial strain C8-1 for peat-reek breath.GC-MS qualification results show that bacterial strain C8-1 can generate a few class main volatile chemical combination
Object, benzaldehyde and native monosodium glutamate, elemene, elemol, a- eucalyptols.Wherein, benzaldehyde has fragrance breath, in microorganism relatively often
See, but yield is not high；Native monosodium glutamate is the common compounds that actinomyces generate, itself has fishy smell.Elemene, which has, inhibits cancer cell
Effect, be the main component in current a kind of listing anticarcinogen, there is higher economic value, particularly microorganism to be independently metabolized
It generates.Elemol is commonly used for flavor of raw material.Eucalyptol has certain fragrance in itself, and can make fixastive to use.The bacterial strain can
In the industrial production that tobacco aromaticss are prepared for fermentation, to expanding the rich of tobacco aromaticss, meet production of cigarettes demand tool
There is good application prospect.
Show that the molten bacillus C8-1 is a kind of by Microbiological Characteristics analysis and 16S rRNA gene sequencings
New bacterium, 16S rRNA gene nucleotide series are as shown in sequence table.
The molten bacillus C8-1 well-grown, Gram's staining on most of culture mediums are negative, in ISP 2
It is smooth that surface is formed on (International Streptomyces Project) culture medium, neat in edge, intermediate projections
Yellow color colonies.Bacterial strain shows growth characteristics in the range of 10-40 DEG C, pH 6.0-9.0, is resistant to 1% NaCl.It is dry
Casein, starch, gelatin liquefaction, polysorbas20,40,60 and 80 hydrolysis are positive, cellulose hydrolysis, H2The generation of S is negative.Energy
Utilize glucose, galactolipin, fructose, mannose, sodium acetate, acetoacetate, sucrose, N- acetyl-d-glucosamine, Song Ertang, wheat
Bud sugar.The polar lipid that cell membrane contains mainly includes cardiolipin, phosphatidyl glycerol, phosphatidyl-ethanolamine and phosphatidyl first
Ethylethanolamine.Breathing quinone is CoQ8 (Q-8).Main aliphatic acid is iso-C15:0,iso-C17:0and iso-C17:1ω9c。
The beneficial effects of the invention are as follows：
1. Microbiological Characteristics are analyzed and 16S rDNA gene sequencings show that the molten bacillus C8-1 is a kind of new
Bacterium, experiment prove the bacterial strain can effectively improve flue-cured tobacco interior quality, increase tabacco fragrance.The present invention is successfully by biological skill
Art is introduced into tobacco leaf production, is an important breakthrough of tobacco leaf production technology.
2. by improving quality of tobacco, tobacco leaf usability is greatly improved.Accelerating fermentation of leaf tobacco by use improves tobacco leaf degree quality,
Increase tobacco leaf soluble reducing sugars content, and protein, cellulose, total nitrogen, nicotine content decline, sugared nitrogen ratio, sugared alkali ratio and
The ratio of nitrogen base ratio rises, and intrinsic chemical ingredient is more coordinated, and tobacco leaf suction quality is obviously improved.
3. simple for process, operation is easy, at low cost, suitable for promoting and applying, has product to cigarette industry is promoted to develop in a healthy way
Pole meaning.
The explanation of preservation biomaterial
In September in 2017 28 days, it is common to be preserved in China Committee for Culture Collection of Microorganisms for the bacterial strain of the present invention
Microorganism center CGMCC, the centre address：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, Chinese Academy of Sciences's microbe research
Institute, the strain classification are named as：Molten bacillus (Lysobactersp.) C8-1, deposit number CGMCC No.1.16271.
Specific embodiment
In order to make the purpose , technical scheme and advantage of the present invention be clearer, by the following examples to the present invention
It is described in further detail.Specific embodiment described herein is only not to the technology of the present invention to explain the present invention
The restriction of scheme.
Embodiment 1
--- applications of the molten bacillus C8-1 in quality of tobacco is promoted is as follows：
(1) inclined-plane culture：Culture medium：Glucose 0.5g；Yeast extract 0.5g；Peptone 0.5g；Acid hydrolyzed casein 0.5g；
Soluble starch 0.5g；Sodium Pyruvate 0.3g；Dipotassium hydrogen phosphate 0.3g；Magnesium sulfate 0.05g；Agar 15g；Distilled water 1000mL,
pH7.2；Cultivation temperature is 30 DEG C, incubation time 7d.
(2) seed culture：It is accessed in seed liquor from inclined-plane picking part mycelium, shaking flask culture 36 hours.Seed culture
Base：Dextrin 120g；Soy meal 40g；Yeast extract 2g；Tryptophan 0.5g；Beta-alanine 5g；Magnesium sulfate 0.5g；Ammonium phosphate 0.2g；It steams
Distilled water 1000mL, pH7.2, cultivation temperature is 30 DEG C.
(3) fermented and cultured：It is connected in fermentation medium by 10% inoculum concentration, shaking flask culture 6d.Culture medium：Soy meal
10g；Glucose 10g；Peptone 3g；Sodium chloride 2.5g；Calcium carbonate 2g；Distilled water 1000mL, pH7.2, cultivation temperature is 30 DEG C,
Incubation time is 6d.
(4) fermentation culture 1L is taken, 10min is centrifuged with 3500r/min, removes supernatant, is precipitated with sterile water washing, centrifugation,
Supernatant is removed, is repeated 2 times, finally with 100mL sterile water shaken wells, 10 times of dilution is microbial inoculums during use.
(5) tobacco fermentation
The red big C2F tobacco leaves of Kunming K326B2F and Kunming are used as process object.Concentration for the treatment of is to spray bacterium per 1kg pipe tobaccos
Dosage 10mL, control group spray equivalent sterile water, repeat three times.By treated, tobacco sample is put into 22 DEG C, humidity 60%
Fermentation 48h, takes sample to survey its main chemical compositions, data measured, which is averaged, to be analyzed in climatic chamber.
--- sample treatment and Chemical Components of Tobacco Leaves analysis
1) sample treatment：Tobacco sample is taken, is first crushed with Cyclone mill, offal sample dry about 3h (moisture content at 40 DEG C
6%-8%), 40 mesh sieve is crossed, sample bottle is packed into, covers tightly bottle cap, stored protected from light under room temperature.
2) Chemical Components of Tobacco Leaves is analyzed：Nicolet Antaris type ft-nir spectrometers are equipped with TQ
6.2 quantitative analysis softwares of analyst (Thermo Nicolet companies of the U.S.).
--- chemical constituents determination result
The chemical composition testing result of tobacco leaf is following (referring to table 1, table 2), the experimental results showed that, in terms of single compound,
Significant change has occurred in the tobacco leaf intrinsic chemical ingredient handled through microbial inoculum.Total reducing sugar, content of reducing sugar are more larger than control increase, always
Nitrogen, cellulose, protein, nicotine content are declined compared with the control.Sugared nitrogen ratio, sugared alkali ratio, nitrogen base ratio make moderate progress.
The test data explanation of system, all kinds of pipe tobaccos handled through microbial inoculum are all indicators are better than control, and each ingredient tends to be flat in pipe tobacco
Weighing apparatus.Be conducive to improve tobacco leaf interior quality, improve tabacco fragrance.
Influence of 1 microbial inoculum of table to Chemical Components of Tobacco Leaves content
Influence of 2 microbial inoculum of table to sugar/nitrogen, sugar/nicotine, nitrogen/nicotine ratio of tobacco leaf
--- processing cigarette sample sensory evaluating smoking
The pipe tobacco of processing group and control group is rolled into cigarette respectively, is put into climatic chamber, in humidity 60%, temperature
After being balanced for 24 hours under the conditions of 22 DEG C, smoked panel test by 12 cigarette professions group of smokeing panel test that expert forms of smokeing panel test.Expert, which smokes panel test, to be recognized
For：Using molten bacillus C8-1 treated tobacco samples, there is the good effect for being obviously improved cigarette perfume, reducing miscellaneous gas, reducing stimulation
Fruit the results are shown in Table 3.
Influence of 3 microbial inoculum of table to sensory evaluating smoking
Embodiment 2
Embodiment 1 is repeated, there is following difference：Concentration for the treatment of is to spray microbial inoculum amount 20mL per 1kg pipe tobaccos.
--- chemical constituents determination result
The chemical composition testing result of tobacco leaf is following (referring to table 4,5), the experimental results showed that, in terms of single compound, warp
Significant change has occurred in the tobacco leaf intrinsic chemical ingredient of microbial inoculum processing.Total reducing sugar, content of reducing sugar are more larger than control increase, total nitrogen,
Cellulose, protein, nicotine content are declined compared with the control.Sugared nitrogen ratio, sugared alkali ratio, nitrogen base ratio make moderate progress.System
The test data explanation of system, all kinds of pipe tobaccos handled through microbial inoculum are all indicators are better than control, and each ingredient tends to balance in pipe tobacco.
Be conducive to improve tobacco leaf interior quality, improve tabacco fragrance.
Influence of 4 microbial inoculum of table to Chemical Components of Tobacco Leaves content
Influence of 5 microbial inoculum of table to sugar/nitrogen, sugar/nicotine, nitrogen/nicotine ratio of tobacco leaf
--- processing cigarette sample sensory evaluating smoking
The pipe tobacco of processing group and control group is rolled into cigarette respectively, is put into climatic chamber, in humidity 60%, temperature
After being balanced for 24 hours under the conditions of 22 DEG C, smoked panel test by 12 cigarette professions group of smokeing panel test that expert forms of smokeing panel test.Expert, which smokes panel test, to be recognized
For：Using molten bacillus C8-1 treated tobacco samples, there is apparent promoting or transferring cigarette perfume, fine and smooth alcohol and flue gas, reduce stimulation, promoted
The good result of Tianjin sweet tea sense, the results are shown in Table 6.
Influence of 6 microbial inoculum of table to sensory evaluating smoking
Embodiment 3
Embodiment 1 is repeated, there is following difference：Concentration for the treatment of is to spray microbial inoculum amount 30mL per 1kg pipe tobaccos.
--- chemical constituents determination result
The chemical composition testing result of tobacco leaf is following (referring to table 7,8), the experimental results showed that, in terms of single compound, warp
Significant change has occurred in the tobacco leaf intrinsic chemical ingredient of microbial inoculum processing.Total reducing sugar, content of reducing sugar are more larger than control increase, total nitrogen,
Cellulose, protein, nicotine content are declined compared with the control.Sugared nitrogen ratio, sugared alkali ratio, nitrogen base ratio make moderate progress.System
The test data explanation of system, all kinds of pipe tobaccos handled through microbial inoculum are all indicators are better than control, and each ingredient tends to balance in pipe tobacco.
Be conducive to improve tobacco leaf interior quality, improve tabacco fragrance.
Influence of 7 microbial inoculum of table to Chemical Components of Tobacco Leaves content
Influence of 8 microbial inoculum of table to sugar/nitrogen, sugar/nicotine, nitrogen/nicotine ratio of tobacco leaf
--- processing cigarette sample sensory evaluating smoking
The pipe tobacco of processing group and control group is rolled into cigarette respectively, is put into climatic chamber, in humidity 60%, temperature
After being balanced for 24 hours under the conditions of 22 DEG C, smoked panel test by 12 cigarette professions group of smokeing panel test that expert forms of smokeing panel test.Expert, which smokes panel test, to be recognized
For：Using the molten bacillus C8-1 in hole treated tobacco samples, have and be obviously improved that perfume quantity, to increase fragrance rich, reduce thorn
Swash, promote the good result of Tianjin sweet tea sense, the results are shown in Table 9.
Influence of 9 microbial inoculum of table to sensory evaluating smoking
Sequence table
<110>Cigarette industry Co., Ltd in Yunnan
<120>A kind of method that quality of tobacco is promoted using molten bacillus
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 26
<212> DNA
<213>Molten bacillus C8-1 16S rRNA (2 Ambystoma laterale x Ambystoma
jeffersonianum)
<400> 1
rysbactrta bacsrgnsnc taagat 26
Claims (2)
- A kind of 1. method that quality of tobacco is promoted using molten bacillus, it is characterised in that：Molten bacillus is made after microbial inoculum and is total to tobacco leaf With fermentation；The molten bacillus Classification And Nomenclature is molten bacillus (Lysobacter sp.) C8-1, and the bacterial strain was in September in 2017 28 days It is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center CGMCC, deposit number CGMCC No.1.16271。
- 2. the method according to claim 1 that quality of tobacco is promoted using molten bacillus, it is characterised in that：The common hair Ferment is specially：Microbial inoculum is made after the molten bacillus C8-1 is expanded fermented and cultured, weighs the cigarette of pre-balance moisture to 13% Silk, sprays molten bacillus microbial inoculum, and in terms of pipe tobacco weight, every 1000 grams of pipe tobaccos spray microbial inoculum amount as 10~30mL, after then handling Pipe tobacco be placed in 22 DEG C, ferment 48h in the constant-temperature constant-humidity environment of humidity 60%, 120 DEG C drying pipe tobaccos.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108903049A (en) * | 2018-09-14 | 2018-11-30 | 四川省食品发酵工业研究设计院 | A method of using biotechnology alcoholization fermentation " fresh and sweet fragrant " type cured tobacco leaf |
| CN109317093A (en) * | 2018-09-18 | 2019-02-12 | 云南中烟工业有限责任公司 | A kind of fermentation carbonization phoenix tail tea granule and its preparation method and application |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101824391A (en) * | 2010-02-02 | 2010-09-08 | 红云红河烟草(集团)有限责任公司 | Bacillus pumilus and application thereof |
| CN104164372A (en) * | 2014-08-19 | 2014-11-26 | 云南中烟工业有限责任公司 | Lodderomyces elongisporus and application thereof |
| CN107151636A (en) * | 2017-05-10 | 2017-09-12 | 云南中烟工业有限责任公司 | Move application of the coccus in lifting quality of tobacco in cave |
-
2017
- 2017-12-28 CN CN201711452343.1A patent/CN108175122A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101824391A (en) * | 2010-02-02 | 2010-09-08 | 红云红河烟草(集团)有限责任公司 | Bacillus pumilus and application thereof |
| CN104164372A (en) * | 2014-08-19 | 2014-11-26 | 云南中烟工业有限责任公司 | Lodderomyces elongisporus and application thereof |
| CN107151636A (en) * | 2017-05-10 | 2017-09-12 | 云南中烟工业有限责任公司 | Move application of the coccus in lifting quality of tobacco in cave |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108903049A (en) * | 2018-09-14 | 2018-11-30 | 四川省食品发酵工业研究设计院 | A method of using biotechnology alcoholization fermentation " fresh and sweet fragrant " type cured tobacco leaf |
| CN109317093A (en) * | 2018-09-18 | 2019-02-12 | 云南中烟工业有限责任公司 | A kind of fermentation carbonization phoenix tail tea granule and its preparation method and application |
| CN109317093B (en) * | 2018-09-18 | 2021-07-09 | 云南中烟工业有限责任公司 | Fermented carbonized phoenix tail tea particles and preparation method and application thereof |
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Application publication date: 20180619 |