CN109480328A - It is a kind of for improving the complex micro organism fungicide of quality of tobacco - Google Patents
It is a kind of for improving the complex micro organism fungicide of quality of tobacco Download PDFInfo
- Publication number
- CN109480328A CN109480328A CN201811268889.6A CN201811268889A CN109480328A CN 109480328 A CN109480328 A CN 109480328A CN 201811268889 A CN201811268889 A CN 201811268889A CN 109480328 A CN109480328 A CN 109480328A
- Authority
- CN
- China
- Prior art keywords
- smxp
- bacillus
- culture
- bacterial strain
- micro organism
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
- A24B15/18—Treatment of tobacco products or tobacco substitutes
- A24B15/20—Biochemical treatment
-
- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B3/00—Preparing tobacco in the factory
- A24B3/12—Steaming, curing, or flavouring tobacco
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Tropical Medicine & Parasitology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention belongs to raw material of cigarette to produce and process technical field, and in particular to a kind of to utilize the complex micro organism fungicide patent application matters for being used to improve quality of tobacco prepared by specified microorganisms bacterial strain.Effective component is the viable bacteria of bacillus thuringiensis bacterial strain XC-3, strain of i (bacillus) pumilus SMXP-3, bacillus subtilis strain SMXP-58 in the composite bacteria agent, which is each mixed compound of bacteria liquid culture.In general, the present invention compounds exploitation by the screening of specific bacterial strain and complex microorganism preparations, devise the technical solution of relatively simple complex microorganism preparations processing reconstituted tobacoo, so that include process flow relatively simple by the present invention, processing cost is lower, and process cycle is shorter, can obviously reduce starch, protein and pectin content in tobacco leaf, and improve the technological merits such as quality of tobacco, thus there is preferably application value in field of cigarette producing technology.
Description
Technical field
The invention belongs to raw material of cigarette to produce and process technical field, and in particular to a kind of made using specified microorganisms bacterial strain
The standby complex micro organism fungicide patent application matters for being used to improve quality of tobacco.
Background technique
Tobacco is one of the Important Economic crop in China, is occupied an important position in national economy.And tobacco leaf is tobacco
The basis of industry development, constantly improve quality of tobacco level are one of important goals of leaf tobacco production, " improve quality, optimization knot
Structure " is also all the emphasis of tobacco leaf work all the time.
Pectin, starch, protein etc. are the main macromolecular chemistry material compositions in tobacco leaf.Wherein pectin is to constitute to plant
The main matter of object cell wall has very direct influence to the flavor and taste of the flammability of tobacco product and cigarette, is to burn and suck
Throat generates the main reason for spine sense and bad smell in the process, to generate side effect to cigarette quality；And content of starch
Then closely related with tobacco leaf flammability and suction taste, starch degradation not exclusively then significantly restricts tobacco product flammability, and generates not
Good smell influences whole cigarette quality；Protein is then the important chemical component in tobacco leaf, the height and cigarette quality of content
It is closely related.The excessively high suction quality for tobacco product of protein content be it is unfavorable, can be generated when burning and sucking as burning
The stink of feather, while can also generate pungent, bitter feeling.
In the prior art, be stablize promoted and improve quality of tobacco, generally by 1 ~ 3 year or so natural alcoholization mode come
Processing.But this working process mode, since the time cycle is longer, thus is limited more.And on the other hand, due to starch, egg
The structures of matter such as white matter, pectin are relatively stable, are difficult to degrade in its natural state, therefore often also need when post-production with unification
Fixed artificial treatment mode.In the prior art, a kind of mode is to utilize the processing of particular organisms enzyme rapid enzymolysis, but this processing side
For formula usually along with the reduction of cigarette quality, another way is then by microbial strains fermentation process, thus ensuring cigarette
Macromolecular chemistry material composition is preferably reduced on the basis of grass product matter.But in practical application, produced since different microorganisms bacterial strain is metabolized
Object is different, and different Treatment technique for processing need, thus need to often carry out different strain, different strains are used in compounding, but not
Same strain, different strains due to growth characteristics demand difference, still for the application effect after can compounding and compounding
It need to conduct further research and inquire into.
Summary of the invention
On the basis of the screening of existing microbial strains, inventor is with the bacillus thuringiensis bacterial strain that early period, screening obtained
Based on XC-3, strain of i (bacillus) pumilus SMXP-3 and bacillus subtilis strain SMXP-58, by by its compound system
For at a kind of comprehensive complex micro organism fungicide, when being used for working process tobacco leaf, one is shown between different microorganisms bacterial strain
Fixed synergistic function effectively reduces Starch in Tobacco, protein and pectin content, has improved the entirety of tobacco leaf
Quality shows preferable application effect.
Details are as follows for the technical solution that the application is taken.
A kind of complex micro organism fungicide, effective component are bacillus thuringiensis bacterial strain XC-3, bacillus pumilus bacterium
The viable bacteria of strain SMXP-3, bacillus subtilis strain SMXP-58 are each mixed compound of bacteria liquid culture；
In compound, the active number requirement of each bacterium are as follows:
Bacillus thuringiensis bacterial strain XC-3, (9 ~ 10) × 107A/mL；
Bacillus pumilus SMXP-3, (9 ~ 10) × 107A/mL；
Bacillus subtilis SMXP-58, (9 ~ 10) × 107A/mL.
The bacillus thuringiensis XC-3 bacterial strain belongs to bacillusBacillus, permitted by Henan in 2013
It is separated in prosperous C2F tobacco leaf, preservation title are as follows: bacillus thuringiensis XC-3, systematic name areBacillus thuringiensiXC-3 is now preserved in Wuhan China typical culture collection center (CCTCC), deposit number CCTCC
NO:M 2017352；
The bacillus pumilus SMXP-3 bacterial strain belongs to bacillusBacillus, produced by the Sanmenxia Gorge in 2013
It is separated in redried leaf tobacco, preservation title are as follows: bacillus pumilus SMXP-3, systematic name areBacillus pumilusSMXP-3 is now preserved in Wuhan China typical culture collection center (CCTCC), and deposit number is CCTCC NO:M
2017291；
The bacillus subtilis SMXP-58 bacterial strain, category bacillus subtilis Pseudomonas (Bacillus), it is initially by 2013 Nian Sanmen
It is separated in the redried leaf tobacco that is produced from gorge, preservation title are as follows: bacillus subtilis SMXP-58, systematic name areBacillus subtilisSMXP-58 is now preserved in Wuhan China typical culture collection center (CCTCC), deposit number
For CCTCC NO:M 2016083.
The preparation method of the complex micro organism fungicide, specifically comprises the following steps:
(1) bacillus thuringiensis XC-3 active microbial inoculum is prepared
Bacillus thuringiensis XC-3 bacterial strain is placed on LB plate, under the conditions of 37 DEG C after 72 h of activation culture, collection is cultivated
Bacterium colony, be inoculated in LB liquid medium, 37 DEG C of shaking table cultures are used as seed liquor overnight；
Seed liquor is transferred LB liquid medium by 1% volume ratio, 30 DEG C, under the conditions of 150 rpm culture to OD600=2.0 or so
As fermentation liquid；
3600 rpm of fermentation liquid is centrifuged 10min, abandons supernatant, sterile water is then added and is sufficiently suspended, viable count is adjusted
For (10 ~ 11) × 107A/mL is spare；
(2) bacillus pumilus SMXP-3 active microbial inoculum is prepared
Bacillus pumilus SMXP-3 bacterial strain is placed on LB plate, under the conditions of 37 DEG C after 72 h of activation culture, collection is cultivated
Bacterium colony, be inoculated in LB liquid medium, 37 DEG C of shaking table cultures are used as seed liquor overnight；
Seed liquor is transferred LB liquid medium by 1% volume ratio, 30 DEG C, under the conditions of 150 rpm culture to OD600=2.0 or so
As fermentation liquid；
3600 rpm of fermentation liquid is centrifuged 10min, abandons supernatant, sterile water is then added and is sufficiently suspended, viable count is adjusted
For (10 ~ 11) × 107A/mL is spare；
(3) bacillus subtilis SMXP-58 active microbial inoculum is prepared
Bacillus subtilis SMXP-58 bacterial strain is placed on LB plate, under the conditions of 37 DEG C after 72 h of activation culture, collection is cultivated
Bacterium colony, be inoculated in LB liquid medium, 37 DEG C of shaking table cultures are used as seed liquor overnight；
Seed liquor is transferred LB liquid medium by 1% volume ratio, 30 DEG C, under the conditions of 150 rpm culture to OD600=2.0 or so
As fermentation liquid；
3600 rpm of fermentation liquid is centrifuged 10min, abandons supernatant, sterile water is then added and is sufficiently suspended, viable count is adjusted
For (10 ~ 11) × 107A/mL is spare；
(4) complex micro organism fungicide is prepared
Prepared active microbial inoculum in step (1), step (2), step (3) is mixed, each bacterium active bacteria in mixture is adjusted
Number touches the mark can be (that is, each bacterium activity bacterium number be (9 ~ 10) × 107A/mL)
Application of the complex micro organism fungicide in tobacco leaf, for Starch in Tobacco of degrading, protein and pectin, application method
Specifically:
1 ~ 3% mass ratio of tobacco leaf (preferably 2.6% mass ratio) is pressed, the complex microorganism preparations prepared are uniformly sprayed on
After mixing, ferment tobacco leaf surface 48 ~ 72 h under conditions of 35 DEG C ~ 39 DEG C of temperature, humidity 70%-75%；Later by tobacco leaf
20 min are acted at 80 DEG C, make bacteria inactivation rate.
The present invention is effect target with macromolecular substances such as the higher starch of content in tobacco leaf, protein and pectin, is passed through
To the screening of existing microbial strains and microbial inoculum compounding, it is prepared for the complex micro organism fungicide with specific degradation purposes.Tentatively
Experiments have shown that the complex micro organism fungicide can preferably reduce starch, protein and pectin in tobacco leaf within a short period of time
Content, and certain synergistic function is shown between different microorganisms strain, applying compared with single bacterial strain has preferably
Degradation effect.For specific data, after process optimization, for Starch in Tobacco degradation rate up to 27.6%, protein degradation rate can
Up to 27.1%, pectin degrading rate shows the technology for preferably reducing Starch in Tobacco, protein and pectin content up to 28.3%
Effect.
In general, the present invention compounds exploitation by the screening of specific bacterial strain and complex microorganism preparations, devise compared with
The technical solution of reconstituted tobacoo is handled for easy complex microorganism preparations, so as to include process flow relatively simple by the present invention,
Processing cost is lower, and process cycle is shorter, can obviously reduce starch, protein and pectin content in tobacco leaf, and improve cigarette
The technological merits such as leaf quality, thus there is preferably application value in field of cigarette producing technology.
Specific embodiment
Explanation is further explained to the application below with reference to embodiment, with regard to following embodiments before introducing specific embodiment
Involved in partial material, experimental facilities situations such as be briefly discussed below.
Tobacco: tobacco leaf is provided in China Tobacco Henan Industrial Co., Ltd, is table mountain C3F of Henan in 2016；
Microbial strains:
Bacillus thuringiensis XC-3 bacterial strain belongs to bacillusBacillus, it is initially by the Xuchang of Henan in 2013 C2F tobacco leaf
Middle separation, preservation title are as follows: bacillus thuringiensis XC-3, systematic name areBacillus thuringiensi
XC-3 is now preserved in Wuhan China typical culture collection center (CCTCC), and deposit number is CCTCC NO:M 2017352；
Bacillus pumilus SMXP-3 bacterial strain belongs to bacillusBacillus, it is initially the redrying produced by the Sanmenxia Gorge in 2013
It is separated in tobacco leaf, preservation title are as follows: bacillus pumilus SMXP-3, systematic name areBacillus pumilus
SMXP-3 is now preserved in Wuhan China typical culture collection center (CCTCC), and deposit number is CCTCC NO:M
2017291；
Bacillus subtilis SMXP-58 bacterial strain, category bacillus subtilis Pseudomonas (Bacillus), it is initially to be produced by the Sanmenxia Gorge in 2013
Redried leaf tobacco in separate, preservation title are as follows: bacillus subtilis SMXP-58, systematic name areBacillus subtilisSMXP-58 is now preserved in Wuhan China typical culture collection center (CCTCC), deposit number CCTCC
NO:M 2016083；
Above-mentioned three kinds of bacterial strains, which belong to disclose, obtains bacterial strain, its backup material is preserved in inventor's collection.
Embodiment 1
Complex micro organism fungicide provided herein, prepares especially by following steps.
(1) bacillus thuringiensis XC-3 active microbial inoculum is prepared
Bacillus thuringiensis XC-3 bacterial strain is placed on LB plate, under the conditions of 37 DEG C after 72 h of activation culture, collection is cultivated
Bacterium colony, be inoculated in LB liquid medium, 37 DEG C of shaking table cultures are used as seed liquor overnight；
Seed liquor is transferred LB liquid medium by 1% volume ratio, 30 DEG C, under the conditions of 150 rpm culture to OD600=2.0 or so
As fermentation liquid；
3600 rpm of fermentation liquid is centrifuged 10min, abandons supernatant, sterile water is then added and is sufficiently suspended, viable count is adjusted
For (10 ~ 11) × 107A/mL is spare.
(2) bacillus pumilus SMXP-3 active microbial inoculum is prepared
Bacillus pumilus SMXP-3 bacterial strain is placed on LB plate, under the conditions of 37 DEG C after 72 h of activation culture, collection is cultivated
Bacterium colony, be inoculated in LB liquid medium, 37 DEG C of shaking table cultures are used as seed liquor overnight；
Seed liquor is transferred LB liquid medium by 1% volume ratio, 30 DEG C, under the conditions of 150 rpm culture to OD600=2.0 or so
As fermentation liquid；
3600 rpm of fermentation liquid is centrifuged 10min, abandons supernatant, sterile water is then added and is sufficiently suspended, viable count is adjusted
It is 11 × 107A/mL is spare.
(3) bacillus subtilis SMXP-58 active microbial inoculum is prepared
Bacillus subtilis SMXP-58 bacterial strain is placed on LB plate, under the conditions of 37 DEG C after 72 h of activation culture, collection is cultivated
Bacterium colony, be inoculated in LB liquid medium, 37 DEG C of shaking table cultures are used as seed liquor overnight；
Seed liquor is transferred LB liquid medium by 1% volume ratio, 30 DEG C, under the conditions of 150 rpm culture to OD600=2.0 or so
As fermentation liquid；
3600 rpm of fermentation liquid is centrifuged 10min, abandons supernatant, sterile water is then added and is sufficiently suspended, viable count is adjusted
It is 11 × 107A/mL is spare.
(4) complex micro organism fungicide is prepared
Prepared active microbial inoculum in step (1), step (2), step (3) is mixed, each bacterium active bacteria in mixture is adjusted
Number reaches 10 × 107A/mL or so.
Embodiment 2
When handling tobacco leaf using complex micro organism fungicide prepared by embodiment 1, to determine most preferably matching for complex micro organism fungicide
Than and its for tobacco leaf specific function and effect, inventor carried out partial orthogonality optimum experimental, detailed process are as follows:
It is mixed after drawing 3 kinds of bacterium solutions by single usage ratio of each active microbial inoculum in tobacco leaf, it is later that mixed bacteria liquid is equal
It is even to be applied in tobacco leaf (each processing tobacco leaf is 100 g), while blank control group and 3 kinds of single bacterium liquid processing groups are set, it
After adjust each experimental group moisture content in leaves content be 25%, be placed in climatic chamber (controlled at 37 DEG C, humidity 75%), hair
It is taken out after 72 h of ferment, 80 DEG C of 20 min of placement, make bacteria inactivation rate in baking oven, while adjusting moisture content in leaves content to suitable model
It encloses, so as to subsequent further detection and analysis.
1 L of table9(33) (percentage is practical application quality of each bacterium solution in stem to orthogonal test factor in table with level
Ratio, that is, assuming that stem dosage is 100g, bacterium solution dosage is 0.6 ~ 1.0g)
Note: A is starch degrading bacteriumBacillus thuringiensiXC-3；B is protein degradation bacteriumBacillus pumilus SMXP-3；C is pectin degrading bacteriumBacillus subtilis SMXP-58
2 L of table9(33) orthogonal array
Note: A is starch degrading bacteriumBacillus thuringiensiXC-3；B is protein degradation bacteriumBacillus pumilus SMXP-3；C is pectin degrading bacteriumBacillus subtilis SMXP-58
Meanwhile blank control group CK(is set with equal amount of distilled water alternative composite microorganism formulation) and 3 kinds of bacterium solutions single processing
Group.
Detection evaluation:
(1) sensory quality assessment
After the completion of processing, tobacco leaf is subjected to chopping processing, cigarette sample is prepared into, after climatic chamber balances, is commented by profession
Committee carries out the indexs such as aroma quality (A), perfume quantity (B), concentration (C), soft and fine degree (D), pleasant impression (E), miscellaneous gas (F), stimulation degree (G)
Evaluation, each index press 9 points of system marking, and sensory evaluating smoking's total score is each subitem score summation.
Evaluation result is as shown in table 3 below.
3 quadrature analysis table of table
Note: A is starch degrading bacteriumBacillus thuringiensiXC-3；B is protein degradation bacteriumBacillus pumilus SMXP-3；C is pectin degrading bacteriumBacillus subtilis SMXP-58。
It can be seen from the data in Table 3 that since the higher the better for score of smokeing panel test, and K3 > K2 > K1, thus select A3 for because
The excellent water of plain A is flat, and it is respectively B1, C3 that similarly the excellent water of B, C are flat, so optimal level group is combined into A3B1C3, i.e. T7 scheme.This side
Case is specially starch degradation bacterium solutionBacillus thuringiensi It is 1.0%, protein degradation bacterium solution that XC-3, which applies ratio,Bacillus pumilusIt is 0.6% that SMXP-3, which applies ratio, pectin degrading bacterium solutionBacillus subtilis SMXP-58 is applied
Adding ratio is 1.0%, i.e., the application ratio of 3 kinds bacterium solutions is 1.0%:0.6%:1.0%, the overall application ratio of complex microorganism preparations
It is 2.6%.
It has further investigated the processing of A3B1C3 compound scheme to compare with 3 kinds of single bacterium agent treatment effects, the results are shown in Table 4.
4 comparative analysis table of table
Note: A is starch degrading bacteriumBacillus thuringiensiXC-3；B is protein degradation bacteriumBacillus pumilus SMXP-3；C is pectin degrading bacteriumBacillus subtilis SMXP-58。
As known from Table 4, A3B1C3 compound scheme processing tobacco sensory quality score is higher than 3 kinds of single bacterium liquid and individually handles cigarette
Leaf aesthetic quality's score can preferably promote tabacum sensory after illustrating bacterium solution compounding.
(2) macromolecular substances assay
Using flow analysis, starch, protein, fruit are carried out to tobacco leaf after prioritization scheme (T5) processing and control group tobacco leaf
Glue assay.The results are shown in Table 5.
The chemical composition change of the processing tobacco leaf of table 5
。
Table 5 shows that by T7 treated Starch in Tobacco degradation rate, protein degradation rate is reachable up to 27.6%
27.1%, pectin degrading rate up to 28.3%, imitate by the technology for showing preferably to reduce Starch in Tobacco, protein and pectin content
Fruit.
In general, the bacterial strains such as XC-3, SMXP-3, SMXP-58 of acquisition are screened in the application, derive from tobacco leaf Central Plains
Have bacterial strain, when using its carry out mixing compounding prepare complex microorganism preparations processing tobacco leaf when, ensure for Starch in Tobacco,
Protein and pectin degrading simultaneously, do not have apparent side effect, can more effectively promote macromolecular substances in tobacco leaf and degrade
All kinds of small-molecule substances for being conducive to improve raw material of cigarette quality are formed, and then reach the harmful components reduced in cigarette smoke,
Improve the purpose of raw tobacco material interior quality.
Claims (6)
1. a kind of complex micro organism fungicide, which is characterized in that effective component is bacillus thuringiensis bacterial strain in the composite bacteria agent
The viable bacteria of XC-3, strain of i (bacillus) pumilus SMXP-3, bacillus subtilis strain SMXP-58, the microbial bacterial agent are each bacterium
The mixed compound of liquid culture.
2. complex micro organism fungicide as described in claim 1, which is characterized in that in compound, the active number requirement of each bacterium are as follows:
Bacillus thuringiensis bacterial strain XC-3, (9 ~ 10) × 107A/mL；
Bacillus pumilus SMXP-3, (9 ~ 10) × 107A/mL；
Bacillus subtilis SMXP-58, (9 ~ 10) × 107A/mL.
3. the preparation method of complex micro organism fungicide as claimed in claim 1 or 2, which is characterized in that specifically comprise the following steps:
(1) bacillus thuringiensis XC-3 active microbial inoculum is prepared
Bacillus thuringiensis XC-3 bacterial strain is placed on LB plate, after activation culture, cultivated bacterium colony is collected, is inoculated in liquid
In body LB culture medium, shaking table culture is used as seed liquor overnight；
Seed liquor is transferred LB liquid medium, culture prepares fermentation liquid；
Fermentation liquid is centrifuged, supernatant is abandoned, sterile water is then added and is sufficiently suspended, adjustment viable count is spare；
(2) bacillus pumilus SMXP-3 active microbial inoculum is prepared
Bacillus pumilus SMXP-3 bacterial strain is placed on LB plate, after activation culture, cultivated bacterium colony is collected, is inoculated in
In LB liquid medium, shaking table culture is used as seed liquor overnight；
Seed liquor is transferred LB liquid medium, culture prepares fermentation liquid；
Fermentation liquid is centrifuged, supernatant is abandoned, sterile water is then added and is sufficiently suspended, adjustment viable count is spare；
(3) bacillus subtilis SMXP-58 active microbial inoculum is prepared
Bacillus subtilis SMXP-58 bacterial strain is placed on LB plate, after activation culture, cultivated bacterium colony is collected, is inoculated in
In LB liquid medium, shaking table culture is used as seed liquor overnight；
Seed liquor is transferred LB liquid medium, culture prepares fermentation liquid；
Fermentation liquid is centrifuged, supernatant is abandoned, sterile water is then added and is sufficiently suspended, adjustment viable count is spare；
(4) complex micro organism fungicide is prepared
Prepared active microbial inoculum in step (1), step (2), step (3) is mixed, each bacterium active bacteria in mixture is adjusted
Number touches the mark.
4. application of the complex micro organism fungicide as claimed in claim 1 or 2 in tobacco leaf, which is characterized in that in tobacco leaf of degrading
Starch, protein and pectin.
5. application of the complex micro organism fungicide as claimed in claim 4 in tobacco leaf, which is characterized in that application method are as follows: press cigarette
The complex microorganism preparations prepared are uniformly sprayed on tobacco leaf surface after mixing, in temperature 35 by 1 ~ 3% mass ratio of leaf
DEG C ~ 39 DEG C, ferment under conditions of humidity 70%-75% 48 ~ 72 h；Later by bacteria inactivation rate.
6. application of the complex micro organism fungicide as claimed in claim 5 in tobacco leaf, which is characterized in that 2.6% mass ratio of tobacco leaf
Using complex microorganism preparations.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811268889.6A CN109480328A (en) | 2018-10-29 | 2018-10-29 | It is a kind of for improving the complex micro organism fungicide of quality of tobacco |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811268889.6A CN109480328A (en) | 2018-10-29 | 2018-10-29 | It is a kind of for improving the complex micro organism fungicide of quality of tobacco |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109480328A true CN109480328A (en) | 2019-03-19 |
Family
ID=65693371
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811268889.6A Pending CN109480328A (en) | 2018-10-29 | 2018-10-29 | It is a kind of for improving the complex micro organism fungicide of quality of tobacco |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109480328A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109370937A (en) * | 2018-10-29 | 2019-02-22 | 河南中烟工业有限责任公司 | A kind of reconstituted tobacoo quality adjustment complex micro organism fungicide |
| CN109370938A (en) * | 2018-10-29 | 2019-02-22 | 河南中烟工业有限责任公司 | A kind of complex micro organism fungicide and its application in offal |
| CN117327618A (en) * | 2023-09-27 | 2024-01-02 | 中国农业科学院烟草研究所(中国烟草总公司青州烟草研究所) | Bacillus thuringiensis and application thereof, composite biological microbial inoculum and preparation method and application thereof |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5314639B2 (en) * | 1973-09-18 | 1978-05-18 | ||
| CN106434450A (en) * | 2016-09-30 | 2017-02-22 | 河南中烟工业有限责任公司 | SMXP-58 strain for degrading pectin in tobacco leaves and application of SMXP-58 strain |
| CN106690402A (en) * | 2017-01-12 | 2017-05-24 | 河南中烟工业有限责任公司 | Application of bacillus subtilis SMXP-58 strain in tobacco stem |
| CN106690395A (en) * | 2017-01-12 | 2017-05-24 | 河南中烟工业有限责任公司 | Bacterium enzyme mixed preparation containing bacillus subtilis bacterial strain SMXP-58 |
| CN107475154A (en) * | 2017-09-12 | 2017-12-15 | 河南中烟工业有限责任公司 | The bacterial strains of SMXP 03 and its application for protein in tobacco leaf of degrading |
| CN107488614A (en) * | 2017-09-12 | 2017-12-19 | 河南中烟工业有限责任公司 | For the bacterial strains of XC 3 for Starch in Tobacco of degrading and its application |
| CN107488613A (en) * | 2017-09-12 | 2017-12-19 | 河南中烟工业有限责任公司 | The bacterial strains of XC 19 1 and its application for cellulose in tobacco leaf of degrading |
-
2018
- 2018-10-29 CN CN201811268889.6A patent/CN109480328A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5314639B2 (en) * | 1973-09-18 | 1978-05-18 | ||
| CN106434450A (en) * | 2016-09-30 | 2017-02-22 | 河南中烟工业有限责任公司 | SMXP-58 strain for degrading pectin in tobacco leaves and application of SMXP-58 strain |
| CN106690402A (en) * | 2017-01-12 | 2017-05-24 | 河南中烟工业有限责任公司 | Application of bacillus subtilis SMXP-58 strain in tobacco stem |
| CN106690395A (en) * | 2017-01-12 | 2017-05-24 | 河南中烟工业有限责任公司 | Bacterium enzyme mixed preparation containing bacillus subtilis bacterial strain SMXP-58 |
| CN107475154A (en) * | 2017-09-12 | 2017-12-15 | 河南中烟工业有限责任公司 | The bacterial strains of SMXP 03 and its application for protein in tobacco leaf of degrading |
| CN107488614A (en) * | 2017-09-12 | 2017-12-19 | 河南中烟工业有限责任公司 | For the bacterial strains of XC 3 for Starch in Tobacco of degrading and its application |
| CN107488613A (en) * | 2017-09-12 | 2017-12-19 | 河南中烟工业有限责任公司 | The bacterial strains of XC 19 1 and its application for cellulose in tobacco leaf of degrading |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109370937A (en) * | 2018-10-29 | 2019-02-22 | 河南中烟工业有限责任公司 | A kind of reconstituted tobacoo quality adjustment complex micro organism fungicide |
| CN109370938A (en) * | 2018-10-29 | 2019-02-22 | 河南中烟工业有限责任公司 | A kind of complex micro organism fungicide and its application in offal |
| CN117327618A (en) * | 2023-09-27 | 2024-01-02 | 中国农业科学院烟草研究所(中国烟草总公司青州烟草研究所) | Bacillus thuringiensis and application thereof, composite biological microbial inoculum and preparation method and application thereof |
| CN117327618B (en) * | 2023-09-27 | 2024-05-14 | 中国农业科学院烟草研究所(中国烟草总公司青州烟草研究所) | Bacillus thuringiensis and application thereof, composite biological microbial inoculum and preparation method and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101824391B (en) | Bacillus pumilus and application thereof | |
| CN103667120B (en) | Bacillus pumilus, method for acquiring strain and application of strain in orientated degradation of nitrosoamine specifically in tobacco | |
| CN102505002B (en) | Micro-organic bacterial strain for quick flavor enhancement of flue-cured tobacco and application thereof in tobacco fermentation | |
| CN103169153B (en) | Method for improving internal quality of low-class tobacco through solid fermentation | |
| CN104223352B (en) | A kind of Radix Glycyrrhizae fermentation gained tobacco aromatics using and application thereof | |
| CN106723311A (en) | Application of the bacillus subtilis in terms of cellulose in degrading tobacco product | |
| CN107488614B (en) | XC-3 strain for degrading starch in tobacco leaves and application thereof | |
| CN106723310B (en) | Application of the bacillus subtilis in degrading tobacco product in terms of protein | |
| CN109480328A (en) | It is a kind of for improving the complex micro organism fungicide of quality of tobacco | |
| CN102217794B (en) | Tobacco leaf biochemical additive and preparation method and application thereof | |
| WO2003094639A1 (en) | Microorganism reducing the amount of nitrosamine and method of reducing the amount of nitrosamine using the microorganism | |
| CN107475154A (en) | The bacterial strains of SMXP 03 and its application for protein in tobacco leaf of degrading | |
| Yao et al. | Screening of Cellulase-producing Bacteria and their Effect on the Chemical Composition and Aroma Quality Improvement of Cigar Wrapper Leaves. | |
| CN105768199B (en) | A kind of compound formulation for being used to accelerate tobacco mellowing | |
| CN102860580A (en) | Biological agent for improving tobacco quality on upper portion of flue-cured tobacco, preparation method and application of biological agent | |
| CN107151636A (en) | Move application of the coccus in lifting quality of tobacco in cave | |
| CN110713957B (en) | Geobacillus altitudinis J54 and application thereof | |
| CN107354109B (en) | Aroma-imparting compound microbial preparation for rapid fermentation of tobacco leaves and application thereof | |
| CN102399725B (en) | Cigarette group imparting strain, compound microbial preparation and application thereof | |
| CN110846252B (en) | Geobacillus altitudinis J45 and acquisition method and application thereof | |
| CN1278635C (en) | Biological agent for reducing specific nitrosamine content which tobacco has, preparation method and use | |
| CN108220344A (en) | A kind of tobacco aromaticss prepared using microbial fermentation Stauntonia latifolia and its application | |
| CN107495462A (en) | A kind of method for lifting cigarette quality, reducing harmful substance | |
| CN109182189B (en) | High-yield microbacterium oxydans and application thereof | |
| CN109370937A (en) | A kind of reconstituted tobacoo quality adjustment complex micro organism fungicide |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190319 |
|
| RJ01 | Rejection of invention patent application after publication |