CN107488614A - For the bacterial strains of XC 3 for Starch in Tobacco of degrading and its application - Google Patents
For the bacterial strains of XC 3 for Starch in Tobacco of degrading and its application Download PDFInfo
- Publication number
- CN107488614A CN107488614A CN201710818116.XA CN201710818116A CN107488614A CN 107488614 A CN107488614 A CN 107488614A CN 201710818116 A CN201710818116 A CN 201710818116A CN 107488614 A CN107488614 A CN 107488614A
- Authority
- CN
- China
- Prior art keywords
- tobacco
- starch
- tobacco leaf
- microbial inoculum
- bacterial strains
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
- C12R2001/075—Bacillus thuringiensis
-
- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
- A24B15/18—Treatment of tobacco products or tobacco substitutes
- A24B15/20—Biochemical treatment
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Biomedical Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention belongs to tobacco technical field of microbial fermentation, and in particular to one plant of bacterial strain of bacillus thuringiensis XC 3 for Starch in Tobacco of degrading and its application in tobacco fermentation.The bacterial strain is bacillus thuringiensis XC 3, is now preserved in Wuhan China typical culture collection center, and deposit number is CCTCC NO：M 2017352.The present invention devises the technical scheme that relatively simple microbial inoculum handles tobacco leaf so that the present invention has by the screening of specific bacterial strain：Technological process is relatively simple, and tobacco leaf processing cost is relatively low, and process cycle is shorter, can obviously reduce the technological merits such as content of starch in tobacco leaf, thus has preferably application value in field of cigarette producing technology.
Description
Technical field
The invention belongs to tobacco technical field of microbial fermentation, and in particular to one plant of Su Yun for Starch in Tobacco of degrading
Golden bacillus XC-3 bacterial strains and its application in terms of tobacco fermentation.
Background technology
Starch is the important carbohydrate accumulated during tobacco growing, is widely present in the cauline leaf of tobacco, it contains
The height of amount and quality of tobacco are closely related.But the too high suction quality for tobacco product of content of starch be it is unfavorable, one
Aspect can influence the speed and completeness burnt and sucked, and another aspect starch can produce in burning to be charred smell and a variety of be harmful into
Point, harmful effect is produced to the color of tobacco.It is generally believed that external sound tobacco content of starch is l% ~ 2%, and China
For 4% ~ 6%, Starch in Flue-cured is generally higher, it has also become restricts one of an important factor for domestic quality of tobacco improves.Therefore, it is sharp
With suitable method by the starch degradation in tobacco leaf into small-molecule substances such as reduced sugars, be improve quality of tobacco effective way it
One.And the hydrolysis of tobacco itself enzyme is relied on merely far from meeting the needs of cigarette industry is to high-quality raw tobacco material,
How content of starch in tobacco leaf is reduced using biotechnology, so as to improve quality of tobacco, it has also become scientific worker is generally closed
The problem of note.
The research of bioanalysis degraded tobacco leaf starch is mainly enzyme preparation method and microbial method, and the latter is so that its cost is cheap, behaviour
Making the advantage such as simple turns into study hotspot, therefore screens specificity microorganism and be applied to tobacco fermentation, in effectively degraded tobacco leaf
Macromolecular substances, the quick quality of tobacco that improves are of great practical significance.
The content of the invention
Present invention aims at provide one plant of bacillus thuringiensis XC- for being capable of efficient degradation Starch in Tobacco material
3 bacterial strains, so as to effectively facilitate the degraded of macromolecules starch in tobacco leaf, improve tobacco leaf overall quality.
The detailed technology scheme that the application is taken is as follows.
One plant of XC-3 bacterial strain for Starch in Tobacco of degrading, the bacterial strain is bacillus thuringiensisBacillus thuringiensi ）, it is initially that its preservation is entitled by being separated in the C2F tobacco leaves of the Xuchang of Henan in 2013：Su Yun gold buds
Spore bacillus XC-3, systematic name areBacillus thuringiensi XC-3, it is preserved in force on June 19th, 2017
Chinese China typical culture collection center（CCTCC）, deposit number is CCTCC NO：M 2017352.
Using the microbial inoculum for degrading prepared by the XC-3 bacterial strains of Starch in Tobacco, be made by the steps and
Into：
（1）Activated spawn, XC-3 bacterial strains are placed on primary dcreening operation solid medium, activation culture, specifically：It is living under the conditions of 37 DEG C
Change 72 h of culture；
（2）Prepare seed liquor, picking step（1）Middle cultivated bacterium colony, is inoculated in liquid seed culture medium, 37 DEG C of shaking table trainings
Support；
（3）Expand culture, by step（2）Middle seed liquor switching liquid secondary screening culture medium, is cultivated to OD600=1.8~2.0；
（4）Microbial inoculum is prepared, by step（3）Middle zymotic fluid centrifugation, abandons supernatant, then adds sterilized water and is suspended, adjusts viable bacteria
Number is（9~10）×107Individual/mL.
In above-mentioned preparation process, medium component unit in terms of g/L,
The primary dcreening operation solid medium forms：Beef extract 5.0, peptone 10.0, NaCl 5.0, soluble starch 20.0, fine jade
Cosmetics 15.0, pH 7.0~7.2；
The liquid seed culture medium forms：Beef extract 5.0, peptone 10.0, NaCl 5.0, pH 7.0~7.2；
The liquid secondary screening culture medium forms：Soluble starch 20.0, bean cake powder 20.0, pH 7.0~7.2.
Application of the microbial inoculum using prepared by XC-3 bacterial strains in tobacco leaf starch of degrading, by the mass ratio of tobacco leaf 2 ~ 4%
Example, tobacco leaf surface is uniformly sprayed on by the microbial inoculum prepared, under the conditions of 35 DEG C ~ 40 DEG C of temperature, the h of the h of fermentation process 36 ~ 72
（It is preferred that under the conditions of 3% application ratio, 37 DEG C of processing 48h）, after the completion of fermentation, then by tobacco leaf heat make bacterium and its production
Raw amylase inactivation（70 ~ 100 DEG C of heating 10 ~ 30min, preferably 80 DEG C heating 20min）.
A kind of method for reducing Starch in Tobacco content, comprises the following steps：
（1）Processing microbial inoculum is prepared, specifically, with bacillus thuringiensis XC-3 fermented and cultureds, being prepared into viable count is（9~
10）×107Individual/mL aqua；
（2）By step（1）In it is prepared processing microbial inoculum uniformly spray under the conditions of pending tobacco leaf surface, 35 DEG C ~ 40 DEG C, ferment
The h of 36 h ~ 72 is handled, after the completion of fermentation, then tobacco leaf, which is heated, inactivates bacterium and its caused amylase.
Using the higher starch of content in tobacco leaf as effect target in the present invention, screened by specific bacterial strain, and specific dose
Amount uses, and preferably reduces the content of starch in tobacco leaf within a short period of time.Preliminary Determination result shows, Starch in Tobacco
Up to 30% or so, tobacco leaf entirety aesthetic quality is also lifted more substantially degradation rate, shows preferable technique effect.
In general, the present invention devises the technology that relatively simple microbial inoculum handles tobacco leaf by the screening of specific bacterial strain
Scheme so that the present invention has：Technological process is relatively simple, and tobacco leaf processing cost is relatively low, and process cycle is shorter, can substantially drop
Content of starch in low tobacco leaf simultaneously improves the technological merits such as quality of tobacco, thus has in field of cigarette producing technology and preferably push away
Wide application value.
Brief description of the drawings
Fig. 1 is aspect graph of the bacillus thuringiensis XC-3 bacterial strains under oil mirror；
Fig. 2 is the phylogenetic evolution tree based on bacillus thuringiensis XC-3 bacterial strain 16S rDNA gene orders structure.
Embodiment
Explanation is further explained to the application with reference to embodiment, with regard to following embodiments before specific embodiment is introduced
In situations such as being related to partial material, experimental facilities be briefly discussed below.
Tobacco：
Tobacco leaf used in bacterial strain screening is the Xuchang of Henan in 2013 C2F tobacco leaves；Tobacco leaf used in the investigation of bacterial strain action effect is 2013
Henan table mountain C3F, is provided by China Tobacco Henan Industrial Co., Ltd.
Embodiment 1
The present embodiment is only briefly discussed below with regard to the screening and qualification process of XC-3 bacterial strains.
XC-3 bacterial strains, obtained by being separated in the C2F tobacco leaves of the Xuchang of Henan in 2013.Its bacterium colony is circular, transparent, wet
Profit, surface sticks shape, the mm of diameter about 3, neat in edge.Form under oil mirror is as shown in figure 1, it is in shaft-like, gram that bacterial strain, which can be observed,
Dyeing is shown as positive.
16S rDNA genetic fragments are used as universal primer, performing PCR and cloning and sequencing are entered to the bacterial strain, 1414 can be obtained
Bp sequences, phylogenetic analysis is carried out, show strain X C-3 and bacillus thuringiensis（Bacillus thuringiensi）'s
Homology is up to 99%, i.e., nearest with the affiliation of bacillus thuringiensis（As shown in Figure 2）.
Physiological and biochemical analysis further is carried out to bacterial strain, as a result as shown in table 1.
The XC-3 bio-chemical characteristics results of table 1
Note：+ represent positive, be or can utilize ,-represent negative, no or can not utilize.
Finally, XC-3 bacterial strain category bacillus is thought in identification（Bacillus）, its systematic name isBacillus thuringiensiXC-3, Wuhan China typical culture collection center is submitted on June 19th, 2017（CCTCC）Preservation（Ground
Location：Wuhan University）, preservation on June 30 in 2017 is accredited as survival, and its deposit number is CCTCC NO：M 2017352.
Embodiment 2
For screening gained XC-3 bacterial strains in embodiment 1 are used for into Starch in Tobacco of degrading, the bacterial strain need to be prepared into first and be easy to
The microbial inoculum used, specific preparation process are as follows.
（1）Activated spawn, XC-3 bacterial strains are placed on primary dcreening operation solid medium, 72 h are cultivated under the conditions of 37 DEG C；
（2）Prepare seed liquor, picking step（1）Middle cultivated bacterium colony, is inoculated in liquid seed culture medium, 37 DEG C of shaking table trainings
Support overnight；
（3）Expand culture, by step（2）Middle seed liquor switching liquid secondary screening culture medium, is cultivated to OD600=2.0 or so；
（4）Microbial inoculum is prepared, by step（3）Middle zymotic fluid centrifugation, abandons supernatant, then adds sterilized water and is suspended, adjusts viable bacteria
Number is 10 × 107Individual/mL or so.
Embodiment 3
Using when prepared microbial inoculum handles tobacco leaf in embodiment 2, concretely comprise the following steps：
When handling tobacco leaf, according to the mass ratio of tobacco leaf 2 ~ 4%（Inoculum concentration）, above-mentioned microbial inoculum is uniformly sprayed on cigarette with sprayer
Leaf surface, it is 25% then to adjust moisture content in leaves content；After being placed under specific temperature conditions certain time of fermenting, 80 DEG C of processing 20
Min, inactivate bacterium and its caused amylase.
To determine the best use of condition of microbial inoculum（Fermentation time, temperature, inoculum concentration）, inventor carried out orthogonal experiment and set
Meter, related experiment process are briefly discussed below.
Tobacco sample：The tobacco leaf after extracting offal is taken, splits slabbing, is then uniformly divided into more parts, every part of 100 g.
Fermentation time, fermentation temperature, inoculum concentration：Respectively using fermentation time, fermentation temperature, inoculum concentration as influence factor, enter
The level design of 3 factor 3 is gone, specific design is as follows：
The factor level table L of table 29（33）
Note：A is fermentation time；B is fermentation temperature；C is inoculum concentration.
According to table 2, Three factors-levels orthogonal has been carried out（Table 3）：
The orthogonal array L of table 39（33）：
Note：A is fermentation time；B is fermentation temperature；C is inoculum concentration；Blank control CK is set simultaneously.
The detection of Starch Content of Tobacco uses tobacco business standard：《Tobacco and the measure continuous stream of tobacco product starch
Dynamic method》（YC/T 216-2013）；
Tobacco sensory quality is evaluated：By tobacco leaf chopping, cigarette specification is made（20+64）The mm of mm × 24.5 cigarette sample,
（22±1）DEG C, relative humidity（60±2）Balanced in % climatic chamber to be evaluated after 48 h.Testing sample is delivered into cigarette in Henan
Technique center, by 11 professional members of smoking of central interior to aroma quality（A）, perfume quantity（B）, concentration（C）, soft and fine degree（D）, pleasant impression
（E）, miscellaneous gas（F）, excitant（G）Evaluated etc. index, each index is given a mark by 9 points of systems, as a result takes its average value.Sense organ
Smoke panel test total score（T）=（A+B）× 2.3 + C × 1.5 + D + E + F + G.
Embodiment 4
Starch Content of Tobacco measurement result in embodiment 3, further carries out range analysis, as a result such as table 4：
The range analysis table of table 4
Note：A is fermentation time；B is fermentation temperature；C is inoculum concentration.
As known from Table 4, optimal level is combined as A2B3C3, i.e., the time is 48 h, fermentation temperature is 37 DEG C, inoculum concentration is
3%.By R values relatively：RA>RC>The influence of RB, i.e. fermentation time to whole action condition is maximum, next to that applying ratio
Example, and the influence of fermentation temperature is minimum.
Because optimal combination is not in 9 groups of orthogonal processing schemes, therefore it is real that checking has been carried out to optimization processing scheme
Test.As a result show, Starch Content of Tobacco is 4.68% after fermentation, with compareing（Content of starch is 6.67%）Compare, degradation rate reaches
29.83%。
Embodiment 5
Tobacco leaf is handled according to above-mentioned optimal case, tobacco sensory quality is evaluated afterwards, the results are shown in Table shown in 5.
The tobacco sensory quality of table 5 evaluates (dividing)
。
As shown in Table 5, after bacterium solution is handled, aroma quality, perfume quantity, flue gas concentration etc. significantly improve tobacco leaf, miscellaneous gas, thorn
Swash property etc. substantially to mitigate, the improvement such as pleasant impression, soft and fine degree is obvious, and tobacco leaf total quality significantly improves（Total score improves 1.81 points）.
In summary, bacterium solution is uniformly sprayed on to tobacco leaf surface to be fermented, in bacterium solution inoculum concentration 3%, temperature is 37 DEG C
Under the conditions of ferment 48 h, the starch degradation rate in tobacco leaf is up to 29.83%；Tobacco leaf entirety aesthetic quality also obtains more obvious
Lifting, thus the present invention is reducing Starch in Tobacco content, has preferably application prospect so as to lift quality of tobacco aspect.
In general, the XC-3 bacterial strains of acquisition are screened in the application, it derives from original bacterial strain in tobacco leaf, when its institute of utilization
When preparing microbial inoculum processing tobacco leaf, ensuring for Starch in Tobacco degraded simultaneously, can be more effective without obvious side effect
Promotion tobacco in macromolecular substances degrade to be formed it is all kinds of be advantageous to improve cigarette quality small-molecule substances, and then reduce
Harmful components in cigarette smoke, improve the purpose of the interior quality of tobacco leaf.Meanwhile it is used for tobacco leaf starch in research before and drops
The bacterial strain of solution is mostly bacillus subtilis, Rhizopus oryzae etc., rarely has the report that bacillus thuringiensis is used for tobacco leaf starch degradation
Road, the present invention provide a new thinking without the optimization suspected of tobacco microorganism fermentation process.
Claims (6)
1. one plant of XC-3 bacterial strain for Starch in Tobacco of degrading, it is characterised in that the bacterial strain category bacillusBacillus,
Preservation is entitled：Bacillus thuringiensis XC-3, systematic name areBacillus thuringiensi XC-3, in 2017
On June 30, in is preserved in Wuhan China typical culture collection center, and deposit number is CCTCC NO：M 2017352.
2. utilize the microbial inoculum for being used to degrade prepared by the XC-3 bacterial strains of Starch in Tobacco described in claim 1, it is characterised in that logical
Following steps are crossed to be prepared：
（1）Activated spawn, XC-3 bacterial strains are placed on primary dcreening operation solid medium, activation culture；
（2）Prepare seed liquor, picking step（1）Middle cultivated bacterium colony, is inoculated in liquid seed culture medium, shaking table culture；
（3）Expand culture, by step（2）Middle seed liquor switching liquid secondary screening culture medium, is cultivated to OD600=1.8~2.0；
（4）Microbial inoculum is prepared, by step（3）Middle zymotic fluid centrifugation, abandons supernatant, then adds sterilized water and is suspended, adjusts viable bacteria
Number is（9~10）×107Individual/mL.
3. the microbial inoculum prepared by XC-3 bacterial strains is utilized as claimed in claim 2, it is characterised in that in terms of g/L,
The primary dcreening operation solid medium forms：Beef extract 5.0, peptone 10.0, NaCl 5.0, soluble starch 20.0, fine jade
Cosmetics 15.0, pH 7.0~7.2；
The liquid seed culture medium forms：Beef extract 5.0, peptone 10.0, NaCl 5.0, pH 7.0~7.2；
The liquid secondary screening culture medium forms：Soluble starch 20.0, bean cake powder 20.0, pH 7.0~7.2.
4. the application described in Claims 2 or 3 using the microbial inoculum prepared by XC-3 bacterial strains in cigarette, it is characterised in that be used for
Degraded Starch in Tobacco.
5. the application using the microbial inoculum prepared by XC-3 bacterial strains in cigarette as claimed in claim 4, it is characterised in that press tobacco leaf
The mass ratio of quality 2 ~ 4%, the microbial inoculum prepared is uniformly sprayed on tobacco leaf surface, under the conditions of 35 DEG C ~ 40 DEG C, fermentation process
The h of 36h ~ 72, after the completion of fermentation, then tobacco leaf, which is heated, inactivates bacterium and its caused amylase.
6. the application using the microbial inoculum prepared by XC-3 bacterial strains in cigarette as claimed in claim 5, it is characterised in that press tobacco leaf
The mass ratio of quality 3%, the microbial inoculum prepared is uniformly sprayed on tobacco leaf surface, under the conditions of 37 DEG C, fermentation process 48h.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710818116.XA CN107488614B (en) | 2017-09-12 | 2017-09-12 | XC-3 strain for degrading starch in tobacco leaves and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710818116.XA CN107488614B (en) | 2017-09-12 | 2017-09-12 | XC-3 strain for degrading starch in tobacco leaves and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107488614A true CN107488614A (en) | 2017-12-19 |
| CN107488614B CN107488614B (en) | 2020-06-23 |
Family
ID=60652192
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710818116.XA Active CN107488614B (en) | 2017-09-12 | 2017-09-12 | XC-3 strain for degrading starch in tobacco leaves and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107488614B (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109370937A (en) * | 2018-10-29 | 2019-02-22 | 河南中烟工业有限责任公司 | A kind of reconstituted tobacoo quality adjustment complex micro organism fungicide |
| CN109480328A (en) * | 2018-10-29 | 2019-03-19 | 河南中烟工业有限责任公司 | It is a kind of for improving the complex micro organism fungicide of quality of tobacco |
| CN110663990A (en) * | 2019-10-21 | 2020-01-10 | 河南中烟工业有限责任公司 | Method for processing inapplicable tobacco leaves and method for evaluating usability of processed tobacco shreds |
| CN112538401A (en) * | 2020-11-19 | 2021-03-23 | 河南中烟工业有限责任公司 | Preparation method of corn spice for cigarettes |
| CN113881603A (en) * | 2021-11-04 | 2022-01-04 | 中国烟草总公司陕西省公司 | Bacillus capable of degrading starch in tobacco leaves |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103756944A (en) * | 2014-02-11 | 2014-04-30 | 河南中烟工业有限责任公司 | Bacterium-enzyme combined preparation containing bacillussubtilis strain xp and application of bacterium-enzyme combined preparation in accelerating starch degradation in tobacco stem |
| CN103756946A (en) * | 2014-02-11 | 2014-04-30 | 河南中烟工业有限责任公司 | Bacterium-enzyme combined preparation containing bacillussubtilis strain xp and application of bacterium-enzyme combined preparation in accelerating starch degradation in tobacco sheet |
| CN103756945A (en) * | 2014-02-11 | 2014-04-30 | 河南中烟工业有限责任公司 | Bacillus subtilis strain xp, bacterium-enzyme combined preparation and application of bacterium-enzyme combined preparation to degradation of starch compound in tobacco product |
-
2017
- 2017-09-12 CN CN201710818116.XA patent/CN107488614B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103756944A (en) * | 2014-02-11 | 2014-04-30 | 河南中烟工业有限责任公司 | Bacterium-enzyme combined preparation containing bacillussubtilis strain xp and application of bacterium-enzyme combined preparation in accelerating starch degradation in tobacco stem |
| CN103756946A (en) * | 2014-02-11 | 2014-04-30 | 河南中烟工业有限责任公司 | Bacterium-enzyme combined preparation containing bacillussubtilis strain xp and application of bacterium-enzyme combined preparation in accelerating starch degradation in tobacco sheet |
| CN103756945A (en) * | 2014-02-11 | 2014-04-30 | 河南中烟工业有限责任公司 | Bacillus subtilis strain xp, bacterium-enzyme combined preparation and application of bacterium-enzyme combined preparation to degradation of starch compound in tobacco product |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109370937A (en) * | 2018-10-29 | 2019-02-22 | 河南中烟工业有限责任公司 | A kind of reconstituted tobacoo quality adjustment complex micro organism fungicide |
| CN109480328A (en) * | 2018-10-29 | 2019-03-19 | 河南中烟工业有限责任公司 | It is a kind of for improving the complex micro organism fungicide of quality of tobacco |
| CN110663990A (en) * | 2019-10-21 | 2020-01-10 | 河南中烟工业有限责任公司 | Method for processing inapplicable tobacco leaves and method for evaluating usability of processed tobacco shreds |
| CN112538401A (en) * | 2020-11-19 | 2021-03-23 | 河南中烟工业有限责任公司 | Preparation method of corn spice for cigarettes |
| CN113881603A (en) * | 2021-11-04 | 2022-01-04 | 中国烟草总公司陕西省公司 | Bacillus capable of degrading starch in tobacco leaves |
Also Published As
| Publication number | Publication date |
|---|---|
| CN107488614B (en) | 2020-06-23 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107488614A (en) | For the bacterial strains of XC 3 for Starch in Tobacco of degrading and its application | |
| CN102505002B (en) | Micro-organic bacterial strain for quick flavor enhancement of flue-cured tobacco and application thereof in tobacco fermentation | |
| CN107488613A (en) | The bacterial strains of XC 19 1 and its application for cellulose in tobacco leaf of degrading | |
| CN107475159A (en) | Bacillus subtilis and its application in Sauce flavor white wine | |
| CN106723310B (en) | Application of the bacillus subtilis in degrading tobacco product in terms of protein | |
| CN112342169B (en) | Bacillus altitudinis and application thereof in prevention and control of cigar fermentation mildew | |
| CN102676417B (en) | Highland bacillus altitudinis and application thereof in artificial ageing of tobacco leaves at upper part of flue-cured tobacco | |
| CN107034162A (en) | A kind of bacillus BA 01 and its application in terms of tobacco | |
| CN107475154A (en) | The bacterial strains of SMXP 03 and its application for protein in tobacco leaf of degrading | |
| CN107267401A (en) | One plant of Mucor bacteria strain and the application on fermentation green brick tea | |
| CN102217794A (en) | Tobacco leaf biochemical additive and preparation method and application thereof | |
| CN102860580A (en) | Biological agent for improving tobacco quality on upper portion of flue-cured tobacco, preparation method and application of biological agent | |
| CN109480328A (en) | It is a kind of for improving the complex micro organism fungicide of quality of tobacco | |
| CN104207324A (en) | Tobacco leaf processing method | |
| CN103451108A (en) | Aureobasidium pullulans OF-01 strain and method for preparing endogenous derived flavor OF tobacco by using same | |
| CN102399725B (en) | Cigarette group imparting strain, compound microbial preparation and application thereof | |
| CN101177669B (en) | Crude enzyme liquid prepared by microorganism and uses thereof | |
| CN112760257A (en) | Lactobacillus plantarum strain YC21 and application thereof | |
| CN108220344A (en) | A kind of tobacco aromaticss prepared using microbial fermentation Stauntonia latifolia and its application | |
| CN107467707A (en) | The method for improving tobacco sheets by paper making method quality using fragrant complex microorganism preparations are assigned | |
| CN109136146B (en) | Tobacco soil bacterium, and separation method and application thereof | |
| CN116355801A (en) | Bacillus bailii and application thereof in fermented bean curd fermentation production | |
| CN110066757A (en) | One plant of pseudomonad for producing feruloyl esterase and its application | |
| CN106579544B (en) | Application of the bacillus subtilis in degrading tobacco product in terms of polyphenols | |
| CN109136100A (en) | One plant of Tabin aspergillus bacterial strain and the application on fermentation green brick tea |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |