CN102250812A - Bacillus subtilis preparation for tobacco stem treatment - Google Patents
Bacillus subtilis preparation for tobacco stem treatment Download PDFInfo
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- CN102250812A CN102250812A CN2011101991686A CN201110199168A CN102250812A CN 102250812 A CN102250812 A CN 102250812A CN 2011101991686 A CN2011101991686 A CN 2011101991686A CN 201110199168 A CN201110199168 A CN 201110199168A CN 102250812 A CN102250812 A CN 102250812A
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Abstract
The invention discloses a microbial preparation for tobacco, a preparation method and an application thereof, and belongs to the technical field of additives. According to the invention, liquid fermentation culture of a producing strain is adopted to obtain the corresponding microbial preparation. The producing strain is bacillus subtilis (Van3), and the strain was preserved in general microbiological culture collection center of China committee for culture collections of microorganisms in Aug 16th, 2010, and has a preservation number of CGMCC No.4091. The microbial preparation of the invention can obviously improve the stem quality such as aroma volume, cheerfulness, and the like, reduces miscellaneous gas and irritation, decreases smoke concentration and strength, makes aftertaste become comfortable, and thus further increases the mixing amount of stems in a cigarette so as to realize the purposes of harm reduction, tar reduction, and cigarette production cost reduction.
Description
Technical field
The invention belongs to the technical field of cigarette additive, more particularly, the present invention relates to a kind of microbial preparation.Also relate to the preparation method of described microbial preparation and the application in tobacco industry thereof simultaneously.
Background technology
Along with the raising of China's cigarette process state of the art and the work of tobacco industry lowering harm and decreasing coking continue carry out, the application of expanded cut stem in cigarette composition is more extensive.After in prescription, adding expanded cut stem,, can reduce the cigarette shreds consumption, not only save the cigarette cost, also can play the effect that reduces tar content in cigarette because its filling value is higher.
Yet, at present comparatively backward to the state of the art of offal comprehensive utilization of resources, idle offal waste is big, the blending proportion of stem in cigarette composition is low, interdependency height to tobacco leaf, the cigarette composition cost is higher, also fails to bring into play better the effect of its upgrading consumption reduction, lowering harm and decreasing coking in cigarette product.Therefore, improve the stem quality, improve the comprehensive utilization ratio of stem, work of cigarette lowering harm and decreasing coking and reduction manufacturing cost are had important practical significance.
On the other hand, the main component of offal is a cell wall substance, accounts for more than 40%, and it comprises xylogen, Mierocrystalline cellulose, hemicellulose and pectin etc.The thermal cracking products of this class material has strong impulse, assorted gas is heavy, choke cough, puckery mouthful, perfume quantity be few, had a strong impact on the smoking quality of cigarette, directly limited stem shared ratio in tobacco leaf formulation.Therefore, reduce the bad breath that the stem burning produces, improving stem suction flavor is the key issue that improves the stem comprehensive utilization ratio.
Recent research shows, handles the blue foreign smell with obvious elimination tobacco leaf by microorganism or biological enzyme, and the effect of abirritate also has certain help to the improvement of tabacco fragrance quality.Utilize lignin-degrading bacteria and degrading enzyme offal to be handled xylon gas, the pungency that can alleviate stem.But in the prior art, about adding the rarely seen report of research that microbial preparation directly acts on stem and improves the stem suction quality.By literature search, do not find the report identical as yet with content of the present invention.
Summary of the invention
The objective of the invention is to deficiency, a kind of new microbial preparation is provided at existing stem treatment technology.
Another object of the present invention provides the preparation method of described microbial preparation.
Further aim of the present invention provides the application of described microbial preparation in stem is handled.
Purpose of the present invention is achieved by following technical proposals.
* except as otherwise noted, percentage ratio of the present invention is mass percent.
A. the invention provides a kind of new microbial preparation, this microbial preparation is to adopt following method to prepare: get the liquid spawn that obtains bacterium Van3 from the separation of flue-cured tobacco blade face, inoculum size according to 0.1% is inoculated in the LB liquid nutrient medium of 200ml, at 37 ℃ of shaking tables, rotating speed 180r/min shaking culture is to OD value=1.5; The centrifugal 10min of 3500rpm/min uses the sterilized water washing precipitation, and is centrifugal, and at last with the vibration of 20mL sterilized water evenly, dilution is required microbial preparation for 10 times during use; Wherein, the liquid spawn of described bacterium Van3 belongs to subtilis through evaluation, this bacterial strain called after Bacillus subtilis.
B. the invention provides a kind of preparation method of described microbial preparation, this method adopts following step:
(1) separates the liquid spawn that obtains bacterium Van3 on the flue-cured tobacco blade face;
(2) get liquid spawn, the inoculum size according to 0.1% is inoculated in the LB liquid nutrient medium of 200ml;
(3) at 37 ℃ of shaking tables, rotating speed 180r/min shaking culture is to OD value=1.5; The centrifugal 10min of 3500rpm/min;
(4) use the sterilized water washing precipitation, centrifugal, then with the vibration of 20mL sterilized water evenly, dilution is microbial inoculum for 10 times during use.
C. the invention provides the application of described microbial preparation in stem is handled.
Compared with prior art, the present invention has following outstanding advantage:
(1) microbial preparation provided by the invention can obviously improve the qualities such as perfume quantity, fragrance of stem, declines such as assorted gas, pungency, and flue gas concentration and strength reduce, and it is comfortable that pleasant impression becomes.
(2) cost of the present invention is low, controllability is strong, simple to operate, be convenient to realize large-scale industrial production, have the excellent development application prospect.
The explanation of preservation biomaterial
The bacterial strain of the subtilis Van3 that the present invention relates to has been kept at China Committee for Culture Collection of Microorganisms common micro-organisms center; Address: China. No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City; Preservation date: on 08 16th, 2010; Preserving number is CGMCC No.4091.
Embodiment
In order to make purpose of the present invention, technical scheme and advantage clearer, reach testing data by the following examples, the present invention is described in further detail.Should be appreciated that specific embodiment described herein only in order to explanation the present invention, and be not intended to limit the scope of the invention.
Embodiment 1
---the test tube slant is cultivated
Adopt Luria-Bertani (LB) substratum, filling a prescription is: peptone 10g, and sodium-chlor 10g, yeast extract 5g, agar 20g, the distilled water constant volume is to 1000ml.Sterilized 25 minutes, and put into the inclined-plane for 121 ℃.Inoculation Van35 cultivates 24h for 28 ℃, obtains the test tube kind.
Embodiment 2
---seed culture
Adopt Luria-Bertani (LB) substratum, filling a prescription is: peptone 10g, and sodium-chlor 10g, yeast extract 5g, the distilled water constant volume is to 1000ml.Sterilized 25 minutes for 121 ℃.Inoculation Van35 test tube kind is cultivated 12h for 28 ℃, obtains liquid spawn.
Embodiment 3
---the preparation microbial preparation
Get liquid spawn, the inoculum size according to 0.1% is inoculated in the LB liquid nutrient medium of 200ml, and at 37 ℃ of shaking tables, rotating speed 180r/min shaking culture is to OD value=1.5.The centrifugal 10min of 3500rpm/min uses the sterilized water washing precipitation, and is centrifugal, and then with the vibration of 20mL sterilized water evenly, dilution is microbial inoculum for 10 times during use.
Application Example
---microbial preparation is to the effect of stem
With the microbial preparation for preparing stem is handled, concrete experimental procedure is as follows:
1) takes by weighing the stem 100g of water ratio at 11%-13%.
2) get microbial inoculum 20ml, with miniaturised nebuliser it is sprayed onto on the 100g stem equably, sealing divides evaporation to prevent water.
3) sterilized water that directly sprays equivalent in contrast.
4) sample is enclosed sealing bag, divide evaporation, 30 ℃ of constant temperature 6h to prevent water.
5) stem is placed 120 ℃ of baking ovens dry, make water ratio reach 11%-13%.
6) with stem be 60% climatic chamber balance 48h in 22 ℃ of relative humidity.
7) take out the part pipe tobacco, detect main chemical compositions content and flavor matter.
8) remaining pipe tobacco is rolled into cigarette by hand and prop up, sample and contrast random number, the group of smokeing panel test that is made up of the expert that smokes panel test carries out the sensory evaluating smoking.
The detected result of routine chemical components shows: after described microbial preparation was handled, bigger change had taken place in the content of the biomacromolecule compound of stem, and wherein reducing sugar and sugar contents significantly improve, and nicotine content descends.
The aroma component detected result shows: after microbial preparation was handled, the aroma component of stem comprised that the content of dorinone, spiceleaf acetone, phenylcarbinol, phenylethyl alcohol, furfural, dihydroactinidiolide, vanillin food grade,1000.000000ine mesh etc. significantly improves than contrast.
Sensory evaluating smoking result shows: stem after microbial preparation is handled, declines such as assorted gas, pungency, perfume quantity, fragrance contrast significantly promote, flue gas concentration and strength reduce, it is comfortable that pleasant impression becomes.
Test-results shows that the microbial preparation of this invention has obvious reduction stem pungency, covers the effect of assorted gas, fine and smooth pure and mild flue gas, can reach to improve the stem suction quality, improves the purpose of mixing dosage of stem in cigarette.
Claims (3)
1. microbial preparation, it is characterized in that, this microbial preparation is to adopt following method to prepare: get that to separate the liquid spawn that obtains bacterium Van3 from the flue-cured tobacco blade face be raw material, inoculum size according to 0.1% is inoculated in the LB liquid nutrient medium of 200ml, at 37 ℃ of shaking tables, rotating speed 180r/min shaking culture is to OD value=1.5; The centrifugal 10min of 3500rpm/min uses the sterilized water washing precipitation, and is centrifugal, and then with the vibration of 20mL sterilized water evenly, dilution is required microbial preparation for 10 times during use; Wherein, the liquid spawn of described bacterium Van3 belongs to subtilis through evaluation, this bacterial strain called after Bacillus subtilis.
2. the preparation method of the described microbial preparation of claim 1, this method adopts following step:
(1) separates the liquid spawn that obtains bacterium Van3 on the flue-cured tobacco blade face;
(2) get liquid spawn, the inoculum size according to 0.1% is inoculated in the LB liquid nutrient medium of 200ml;
(3) at 37 ℃ of shaking tables, rotating speed 180r/min shaking culture is to OD value=1.5; The centrifugal 10min of 3500rpm/min;
(4) use the sterilized water washing precipitation, centrifugal, at last with the vibration of 20mL sterilized water evenly, dilution is required microbial inoculum for 10 times during use.
3. the application of the described microbial preparation of claim 1 in stem is handled.
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| CN2011101991686A CN102250812A (en) | 2011-07-17 | 2011-07-17 | Bacillus subtilis preparation for tobacco stem treatment |
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| CN2011101991686A CN102250812A (en) | 2011-07-17 | 2011-07-17 | Bacillus subtilis preparation for tobacco stem treatment |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102524942A (en) * | 2011-12-15 | 2012-07-04 | 华宝食用香精香料（上海）有限公司 | Method for reducing content of lignin in peduncle shred of tobacco |
| CN103451107A (en) * | 2013-08-06 | 2013-12-18 | 红云红河烟草(集团)有限责任公司 | Microbial tobacco additive for treating cut tobaccos |
| CN103451106A (en) * | 2013-08-06 | 2013-12-18 | 红云红河烟草(集团)有限责任公司 | Microbial preparation for treating tobacco stems and preparation method thereof |
| CN103756944A (en) * | 2014-02-11 | 2014-04-30 | 河南中烟工业有限责任公司 | Bacterium-enzyme combined preparation containing bacillussubtilis strain xp and application of bacterium-enzyme combined preparation in accelerating starch degradation in tobacco stem |
| CN104207324A (en) * | 2013-05-30 | 2014-12-17 | 贵州中烟工业有限责任公司 | Tobacco leaf processing method |
| CN104905396A (en) * | 2015-05-18 | 2015-09-16 | 湖州品创孵化器有限公司 | Tobacco stem microbiological treatment aroma increasing method |
| CN106690395A (en) * | 2017-01-12 | 2017-05-24 | 河南中烟工业有限责任公司 | Bacterium enzyme mixed preparation containing bacillus subtilis bacterial strain SMXP-58 |
| CN106690402A (en) * | 2017-01-12 | 2017-05-24 | 河南中烟工业有限责任公司 | Application of bacillus subtilis SMXP-58 strain in tobacco stem |
| CN107345212A (en) * | 2016-12-30 | 2017-11-14 | 广西中烟工业有限责任公司 | The bacillus and its application in reconstituted tobacoo of one plant of cellulase-producing and laccase |
| CN109602070A (en) * | 2018-10-29 | 2019-04-12 | 河南中烟工业有限责任公司 | Application of the SMXP-58 bacterial strain in terms of reducing tobacco stalk fibre cellulose content |
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Cited By (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102524942B (en) * | 2011-12-15 | 2014-04-09 | 华宝食用香精香料（上海）有限公司 | Method for reducing content of lignin in peduncle shred of tobacco |
| CN102524942A (en) * | 2011-12-15 | 2012-07-04 | 华宝食用香精香料（上海）有限公司 | Method for reducing content of lignin in peduncle shred of tobacco |
| CN104207324A (en) * | 2013-05-30 | 2014-12-17 | 贵州中烟工业有限责任公司 | Tobacco leaf processing method |
| CN104207324B (en) * | 2013-05-30 | 2016-08-10 | 贵州中烟工业有限责任公司 | A kind of Nicotiana tabacum L. processing method |
| CN103451107B (en) * | 2013-08-06 | 2015-07-15 | 红云红河烟草(集团)有限责任公司 | Microbial tobacco additive for treating cut tobaccos |
| CN103451107A (en) * | 2013-08-06 | 2013-12-18 | 红云红河烟草(集团)有限责任公司 | Microbial tobacco additive for treating cut tobaccos |
| CN103451106A (en) * | 2013-08-06 | 2013-12-18 | 红云红河烟草(集团)有限责任公司 | Microbial preparation for treating tobacco stems and preparation method thereof |
| CN103451106B (en) * | 2013-08-06 | 2015-07-15 | 红云红河烟草(集团)有限责任公司 | Microbial preparation for treating tobacco stems and preparation method thereof |
| CN103756944A (en) * | 2014-02-11 | 2014-04-30 | 河南中烟工业有限责任公司 | Bacterium-enzyme combined preparation containing bacillussubtilis strain xp and application of bacterium-enzyme combined preparation in accelerating starch degradation in tobacco stem |
| CN103756944B (en) * | 2014-02-11 | 2015-07-08 | 河南中烟工业有限责任公司 | Bacterium-enzyme combined preparation containing bacillussubtilis strain xp and application of bacterium-enzyme combined preparation in accelerating starch degradation in tobacco stem |
| CN104905396A (en) * | 2015-05-18 | 2015-09-16 | 湖州品创孵化器有限公司 | Tobacco stem microbiological treatment aroma increasing method |
| CN107345212A (en) * | 2016-12-30 | 2017-11-14 | 广西中烟工业有限责任公司 | The bacillus and its application in reconstituted tobacoo of one plant of cellulase-producing and laccase |
| CN107345212B (en) * | 2016-12-30 | 2020-07-07 | 广西中烟工业有限责任公司 | Bacillus for producing cellulase and laccase and application of bacillus in tobacco sheets |
| CN106690395A (en) * | 2017-01-12 | 2017-05-24 | 河南中烟工业有限责任公司 | Bacterium enzyme mixed preparation containing bacillus subtilis bacterial strain SMXP-58 |
| CN106690402A (en) * | 2017-01-12 | 2017-05-24 | 河南中烟工业有限责任公司 | Application of bacillus subtilis SMXP-58 strain in tobacco stem |
| CN109602070A (en) * | 2018-10-29 | 2019-04-12 | 河南中烟工业有限责任公司 | Application of the SMXP-58 bacterial strain in terms of reducing tobacco stalk fibre cellulose content |
| CN109602070B (en) * | 2018-10-29 | 2021-11-19 | 河南中烟工业有限责任公司 | Application of SMXP-58 strain in reducing content of cellulose in tobacco stems |
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