CN109315829A - A kind of fermentation carbonization tobacco leaf particle and its preparation method and application - Google Patents
A kind of fermentation carbonization tobacco leaf particle and its preparation method and application Download PDFInfo
- Publication number
- CN109315829A CN109315829A CN201811088872.2A CN201811088872A CN109315829A CN 109315829 A CN109315829 A CN 109315829A CN 201811088872 A CN201811088872 A CN 201811088872A CN 109315829 A CN109315829 A CN 109315829A
- Authority
- CN
- China
- Prior art keywords
- tobacco leaf
- fermentation
- tobacco
- carbonization
- particle
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24D—CIGARS; CIGARETTES; TOBACCO SMOKE FILTERS; MOUTHPIECES FOR CIGARS OR CIGARETTES; MANUFACTURE OF TOBACCO SMOKE FILTERS OR MOUTHPIECES
- A24D3/00—Tobacco smoke filters, e.g. filter-tips, filtering inserts; Filters specially adapted for simulated smoking devices; Mouthpieces for cigars or cigarettes
- A24D3/06—Use of materials for tobacco smoke filters
- A24D3/14—Use of materials for tobacco smoke filters of organic materials as additive
-
- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
- A24B15/18—Treatment of tobacco products or tobacco substitutes
- A24B15/20—Biochemical treatment
Abstract
The present invention provides a kind of fermentation carbonization tobacco leaf particles and its preparation method and application, and prepared by method comprising the following steps: (1) Tobacco wears the preparation of Salmonella L4-6 microbial inoculum；(2) Salmonella L4-6 microbial inoculum is worn using Tobacco to ferment to tobacco leaf, obtain fermenting tobacco leaf；(3) fermenting tobacco leaf is subjected to carbonization treatment, obtains fermentation carbonization tobacco leaf；(4) fermentation carbonization tobacco leaf powder is placed on fluidized bed dry, spray adhesive, bond powders formation particle continues to be drying to obtain fermentation carbonization tobacco leaf particle.The particle passes through fermentation process, and the irritation of flue gas can be made to mitigate, the rich increase of cigarette cigarette perfume, while having the effect of increase flue gas sweet sense；By carbonization treatment, adsorption specific surface area inside tobacco leaf, suction-operated of the reinforcing particle to cigarette smoke harmful substance can be dramatically increased, while assigning plant granule with special burnt odor taste.
Description
Technical field
The invention belongs to cigarette material technical fields, and in particular to a kind of fermentation carbonization tobacco leaf particle and preparation method thereof and
Using.
Background technique
Cigarette flavor is the core content of cigarette quality, but along with the development of low coking cigarette, cigarette smoking mouthfeel drop
It is low, fragrance deficiency etc. have become tobacco development highlight problem.How to capture influences tobacco aroma quality, the crucial skill of perfume quantity
Art has become the core place for improving production of cigarettes competitiveness.Essence spice for cigarette is added in cigarette shreds, has been a kind of normal
The technological means of rule, required supplement fragrance, but its space for promoting cigarette quality is relatively limited, thus it is non-burning from cigarette
Section is set out, and finds relevant new material to improve, be promoted the fragrance of cigarette, is reduced cigarette smoke irritation and is had become new hair
Exhibition trend.Wherein, some granular materials with porosity and special aroma are added, in cigarette filter rod to improve or be promoted volume
The quality of smoke product is a kind of relatively new, effective research direction.But due in preparation method and raw material selection and group of formula
Face there is also some problems, so that existing granular materials additive advantage fails to give full play of.Such as active carbon, micro mist
Although particle made of the sorptive materials such as silica gel has significant advantage in absorption flue gas harmful substance, perfume (or spice) will also result in simultaneously
A large amount of absorption of taste ingredient, so that cigarette smoking quality significantly reduces.And the plants particles material obtained by granulation, although
The fragrance matter mutually coordinated with cigarette flavor can be introduced into cigarette smoke, but often because the addition of adhesive, causes to plant
Object material hole itself is embedded, and reduces the adsorption site of harmful substance, reduces reducing tar and reducing harm effect.In addition, for original
The selection and processing of material, for the harmony of each plant material and cigarette flavor that the prior art is announced it is still necessary to be promoted, tobacco is fragrant
Gas is abundant not enough, therefore continually looks for the plant material coordinated with tobacco this perfume phase, and need to continue the direction of research.
The content that tradition extracts a variety of aroma substances in fragrance can be increased using microbial fermentation and processing with enzyme preparation, mentioned
It rises that fragrance is rich, and then produces that fragrance is abundant, cigarette of high-quality, meet requirement of the consumer to cigarette quality.It utilizes
Microorganism is fermented to itself having odorous plant material, moreover it is possible to which it is former odorous unique fragrant to be different to assign plant
Taste.
Plant carbide can be such that plant is dehydrated in a short time by high temperature, retain inside plants gap, increase plant to cigarette
While gas noxious substance, not only retain material of vegetable origin part aromatic property, but also assigns material of vegetable origin with special coke
Fragrance.Currently, there is no the correlative studys for utilizing microbial fermentation technology combination carbonization technology to be used for cigarette filter tip particle preparation.
For disadvantages mentioned above, how to select appropriate material of vegetable origin and be modified, is made with making up prior art technology
At deficiency, reducing, cigarette smoke is irritating simultaneously, improves granular materials and is increasing cigarette smoke harmony and promoting the production of body fluid sense
The effect of aspect etc. becomes the technical issues of needs further solve.
Summary of the invention
The present invention is unobvious to flue gas noxious substance effect for existing plant granule, cannot directly assign to cigarette
Perfume (or spice), or it is bad with cigarette flavor harmony the disadvantages of, a kind of fermentation carbonization tobacco leaf particle and its preparation method and application is provided,
By using Tobacco wear Salmonella L4-6 fermentation and carbonization technique plant material is modified, volume can be enhanced by providing one kind
Cigarette lowering harm and decreasing coking effect reduces cigarette smoke irritation, and can enhance cigarette and assign fragrant effect and promote cigarette smoking quality.
* unless otherwise indicated, percentage employed in the present invention is mass percent.
First aspect present invention is related to a kind of Tobacco and wears Salmonella L4-6, is from Kunming, Yunnan tobacco planting base cigarette
It is isolated in careless pedotheque, morphology, Physiology and biochemistry property and 16S rDNA analysis, qualification result table have been carried out to it
Bright its belongs to Tobacco and wears Salmonella, and microbiological classification is named as Tobacco and wears Salmonella Dyellatabacisoli L4-6,
It is protected on June 19th, 2018 in China Committee for Culture Collection of Microorganisms's common micro-organisms center (referred to as: CGMCC)
Hiding, deposit number are CGMCC 1.16273, address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City Chinese Academy of Sciences microbe research
Institute.
The isolated Tobacco of the present invention wears main morphological features and the Physiology and biochemistry property spy of Salmonella L4-6 bacterial strain
Sign are as follows: the well-grown on most of culture mediums, on R2A agar medium, colony edge is neat, intermediate projections, is in yellow.
Gelatin liquefaction, oxidizing ferment, catalase, polysorbas20,40,60 and 80 hydrolysis are positive, Starch Hydrolysis, cellulose hydrolysis, junket
Proteolysis is negative, and does not generate hydrogen sulfide, does not generate melanin.It can be using maltose, trehalose, cellobiose, lactose, sweet
Reveal sugar, glycerol, aspartic acid, glutamic acid, histidine.The polar lipid ingredient of cell membrane mainly includes phosphatidyl glycerol, double phosphatide
Acyl glycerol and phosphatidyl-ethanolamine, the breathing quinone of cell are CoQ8 (Q-8).
The separated obtained Tobacco of the present invention wears the 16S rDNA gene nucleotide series such as sequence table of Salmonella L4-6
Shown, which has submitted international nucleotide sequence database (GenBank), sequence retrieval number: MF370623.
Second aspect of the present invention is related to a kind of fermentation carbonization tobacco leaf particle, is prepared by method comprising the following steps:
(1) Tobacco wears the preparation of Salmonella L4-6 microbial inoculum；
(2) Salmonella L4-6 microbial inoculum is worn using Tobacco to ferment to tobacco leaf, obtain fermenting tobacco leaf；
(3) fermenting tobacco leaf is subjected to carbonization treatment, obtains fermentation carbonization tobacco leaf；
(4) fermentation carbonization tobacco leaf powder being placed in drying on fluidized bed, spray adhesive, bond powders form particle, after
It is continuous to be drying to obtain fermentation carbonization tobacco leaf particle.
Above-mentioned fermentation is carbonized tobacco leaf particle, and step (1) specifically includes: by Tobacco wear Salmonella L4-6 liquid spawn by
10% inoculum concentration is inoculated into fermentation medium, 28 DEG C shaking flask culture 3~7 days, obtain culture solution；Every 1000mL culture solution from
Heart separation, is precipitated with sterile water washing, and finally uniformly with the concussion of 20mL sterile water, 10 times of dilution up to microbial inoculum.
Above-mentioned fermentation carbonization tobacco leaf particle, step (2) specifically include: weighing the cigarette of pre-balance moisture to 11%~14%
Leaf, every 100g tobacco leaf spray Tobacco and wear Salmonella L4-6 microbial inoculum 20mL, the tobacco leaf handled well are placed in 22 DEG C, 60% constant temperature
Ferment 24~72h in constant humidity cabinet, obtains fermenting tobacco leaf.
Above-mentioned fermentation carbonization tobacco leaf particle, step (3) specifically include: fermenting tobacco leaf being put into retort, is carried out to it
Closed heating carbonization treatment, concrete operation step are that furnace temperature is gradually increased to 80 DEG C, are brought rapidly up after drying tobacco of fermenting
To 120~300 DEG C, and 1~6h is kept, obtains fermentation carbonization tobacco leaf.
Above-mentioned fermentation carbonization tobacco leaf particle, step (4) specifically comprise the following steps:
1), the moisture content of detection fermentation carbonization tobacco leaf, by moisture control to 8~12%, be ground into partial size be 100~
160 mesh powders；
2) 90~99 parts of tobacco leaf powder, 1~10 portion of sucrose or mannitol powder, are weighed according to parts by weight, is uniformly mixed, it is standby
With；
3) 5~10 parts of gelatin, 0~5 part of polyvinylpyrrolidone, 0~2 part of sodium carboxymethyl starch, are weighed according to parts by weight,
The binder solution that concentration is 5~12% is configured to distilled water；
4) it, weighs 300~1000g of mixed-powder in step 2) and is placed in fluidized bed and be granulated, sprayed in granulation process viscous
Mixture solution is to get fermentation carbonization tobacco leaf particle.
Above-mentioned fermentation carbonization tobacco leaf particle, step 4) specifically comprise the following steps:
(a) by mixed-powder dry 5 under the conditions of fluidizing 0.12~0.2Bar of air pressure, 50~60 DEG C of gas flow temperature~
15min；
(b) in fluidisation 0.12~0.25Bar of air pressure, 60~80 DEG C of gas flow temperature, 0.08~0.15Bar of atomisation pressure, spray
Under conditions of 8~12g/min of liquid speed rate, the prepared binder solution of step 3), binder solution are applied using top spray mode
Dosage is the 20%~50% of fermentation carbonization tobacco leaf powder dry material weight；
(c) it after adhesive applies, repeats step (a), obtains fermentation carbonization tobacco leaf particle.
Above-mentioned fermentation is carbonized tobacco leaf particle, and the moisture content for the fermentation carbonization tobacco leaf particle that step (c) obtains is 8~
10%, partial size is 20~60 mesh, and shape is shaggy similar round particle.
Third aspect present invention is related to a kind of cigarette filter tip, and it includes the fermentation carbonization tobacco leaves of second aspect of the present invention preparation
Particle.
Above-mentioned cigarette filter tip, 1~2mg of addition fermentation carbonization tobacco leaf particle in every millimeter of cigarette filter tip.
The invention has the following advantages:
(1) present invention ferments to it by the way that tobacco leaf is worn Salmonella L4-6 using Tobacco, after being applied to cigarette filter tip,
During cigarette smoking, flue gas irritation can be made to mitigate, the rich increase of cigarette cigarette perfume, while having and increasing the sweet sense of flue gas
Effect；
(2) present invention carries out carbonization treatment by the tobacco leaf after fermenting, and high temperature makes tobacco leaf cells dehydration in a short time
And be formed, increase adsorption specific surface area inside tobacco leaf, significantly improve fermentation carbonization tobacco leaf particle to the filter efficiency of cigarette smoke,
Can selective absorption cigarette smoke harmful substance, while assign fermentation carbonization tobacco leaf particle with special burnt odor taste；
(3) present invention is not adjusted plant granule production technology, only by the modification to raw material, realizes and improves increasing
Fragrant and tar reduction double effects, raw material are easy to get, low in cost, are easy to implement industrialized production, there is good commercial application value.
Detailed description of the invention
Fig. 1 is that Tobacco of the present invention wears electromicroscopic photograph of the Salmonella L4-6 on R2A culture medium；
Fig. 2 is that Tobacco of the present invention wears Salmonella L4-6 and part related strain according to the building of 16S rDNA gene order
Phylogenetic tree.
Specific embodiment
In order to make the objectives, technical solutions, and advantages of the present invention clearer, with reference to the accompanying drawings and embodiments, right
The present invention is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, and
It is not used in the restriction present invention.As long as in addition, technical characteristic involved in the various embodiments of the present invention described below
Not constituting a conflict with each other can be combined with each other.
Embodiment 1
1. separation, culture and identification that Tobacco wears Salmonella L4-6
1.1 Tobaccos wear the separation of Salmonella L4-6
Tobacco sample is taken from Kunming, Yunnan tobacco planting base, plastic bag sealing is taken back, and 4 DEG C save for use.Accurately
10g soil sample is weighed, 90mL sterile water, 30 DEG C, 180rpm shaking table concussion 30min is added；With sterile water by concentration dilution to 10-1、
10-2、10-3、10-4、10-5Times, take 0.2mL to be coated on LB plating medium respectively the bacterium solution of 5 concentration, each concentration
3 parallel tests are arranged in bacterium solution, cultivate 48h at 30 DEG C, from picking difference single colonie on the plate of every part of soil sample, train in LB plate
It supports and glycerol is added by bacterium solution volume 20% after purification on base, be placed in -80 DEG C of refrigerators and save backup.
Isolated microorganism is inoculated in LB liquid medium, 30 DEG C, 160rpm shaking table shaken cultivation respectively, is trained
Supporting to bacterial concentration is OD=2.0, spare as seed liquor.The seed liquor of each strain to be tested is seeded in screening by 1%
In culture medium, in 30 DEG C, 160rpm shaking table shaken cultivation 3-5 days, fermentation liquid appearance and aroma change situation, screening are observed daily
Obtain one plant of microorganism that can generate fragrance, number L4-6.
1.2 Tobaccos wear the identification of Salmonella L4-6
Morphology, Physiology and biochemistry property and 16S rDNA analysis, qualification result table are carried out to the bacterial strain L4-6 isolated and purified
The bright Tobacco that belongs to wears Salmonella, and microbiological classification is named as Tobacco and wears Salmonella Dyellatabacisoli L4-6.
It is as shown in Fig. 1 that Tobacco wears electromicroscopic photograph of the Salmonella L4-6 on R2A culture medium.
Tobacco wears Salmonella L4-6 well-grown on most of culture mediums, on R2A agar medium, colony edge
Neatly, intermediate projections are in yellow.Gelatin liquefaction, oxidizing ferment, catalase, polysorbas20,40,60 and 80 hydrolysis are positive, form sediment
Powder hydrolysis, cellulose hydrolysis, casein hydrolysis are negative, do not generate hydrogen sulfide, do not generate melanin.Maltose, sea can be utilized
Algae sugar, cellobiose, lactose, mannose, glycerol, aspartic acid, glutamic acid, histidine.The polar lipid ingredient of cell membrane is main
Including phosphatidyl glycerol, cardiolipin and phosphatidyl-ethanolamine, the breathing quinone of cell is CoQ8 (Q-8).
The physiological and biochemical property that Tobacco wears Salmonella L4-6 is as shown in table 1:
1 Tobacco of table wears the physiological and biochemical property of Salmonella L4-6
| Experiment | Growth response | Experiment | Growth response |
| Gelatin liquefaction | +++++ | Polysorbas20,40,60, and 80 | +++++ |
| Starch Hydrolysis | -- | Grown on cellulose | -- |
| Cellulose hydrolysis | -- | Alkaline phosphatase | ++++ |
| Casein hydrolysis | -- | Valine arylamine enzyme | ++++ |
Note: "+" indicates that result is the positive, and "-" indicates that result is feminine gender
The carbon nitrogen utilization power that Tobacco wears Salmonella L4-6 is as shown in table 2:
2 Tobacco of table wears the carbon nitrogen source utilization power of Salmonella L4-6
| Utilization of carbon source | As a result | Carbon source or nitrogen source utilize | As a result |
| Dextrin | + | N- acetyl-d-glucosamine | + |
| Maltose | + | Mannitol | + |
| Trehalose | + | Fructose | + |
| Cellobiose | + | Galactolipin | + |
| Sucrose | - | Rhamnose | - |
| Gossypose | - | Alanine | - |
| Melibiose | + | Histidine | + |
Note: "+" indicates that result is the positive, and "-" indicates that result is feminine gender
Tobacco wears the 16S rDNA partial sequence of Salmonella L4-6 as shown in Detailed description of the invention, the sequence and GenBank number
BLAST comparative analysis is carried out according to the known array in library, and obtains the 16S rDNA gene order of related species, structure from database
Phylogenetic tree is built, sees attached drawing 2.It is found through comparative analysis, Tobacco of the invention wears Salmonella L4-6 and bacterial strain (Dyella
soli JS12-10T) affiliation is nearest, forms independent branch on systematic evolution tree, it is comprehensive morphological, Physiology and biochemistry, thin
The features such as born of the same parents' chemistry and phylogenetic analysis, the two have obvious gap again, show that Tobacco of the invention wears Salmonella L4-6 and is
One new species, is named as Dyellatabacisoli.
Tobacco wears Salmonella L4-6 in the 16S rDNA gene nucleotide series accession number of GenBank database
MF370623, the deposit number of China Committee for Culture Collection of Microorganisms's common micro-organisms center are CGMCC 1.16273.
The phylogenetic tree that Tobacco wears the 16S rDNA gene order building of Salmonella L4-6 and related species is as shown in Fig. 2.
Embodiment 2
1. the culture that Tobacco wears Salmonella L4-6
(1) test tube slant culture
Using slant preservation culture medium, culture medium is R2A agar medium, the formula of culture medium are as follows: glucose 0.5g, ferment
Female cream 0.5g, peptone 0.5g, acid hydrolyzed casein 0.5g, soluble starch 0.5g, Sodium Pyruvate 0.3g, dipotassium hydrogen phosphate
0.3g, magnesium sulfate 0.05g, agar 15g, distilled water constant volume to 1000mL, pH7.2.Culture medium is sterilized 25 points at 121 DEG C
Clock is put into inclined-plane, and inoculation Tobacco is worn Salmonella L4-6 bacterial strain, cultivated 1 week at 28 DEG C, obtains test tube strains；
(2) seed culture
Using seed culture medium, the formula of seed culture medium are as follows: dextrin 120g, soy meal 40g, yeast extract 2g, tryptophan
0.5g, Beta-alanine 5g, magnesium sulfate 0.5g, ammonium phosphate 0.2g, distilled water constant volume to 1000mL, pH7.2.By culture medium 121
It sterilizes 25 minutes, is accessed in seed liquor from picking part mycelium on the test tube slant of step (1), 28 DEG C of shaking flask cultures at DEG C
36h obtains liquid spawn；
(3) Tobacco wears the preparation of Salmonella L4-6 microbial inoculum
Liquid spawn made from step (2) is transferred in fermentation medium by 10% inoculum concentration, 28 DEG C of shaking flask cultures 6
It, gained culture solution is centrifuged 10min with 3500r/min, is precipitated with sterile water washing, and precipitating 20mL sterile water shakes uniformly,
10 times of dilution are that Tobacco wears Salmonella L4-6 microbial inoculum.
Fermentative medium formula are as follows: soy meal 10g, glucose 10g, peptone 3g, sodium chloride 2.5g, calcium carbonate 2g steam
Distilled water constant volume sterilizes 25 minutes to 1000mL, pH7.2,121 DEG C, both obtains culture medium.
2. tobacco fermentation
The tobacco leaf 1200g for weighing pre-balance moisture to 13% sprays Tobacco and wears Salmonella L4-6 microbial inoculum 240mL, will
The tobacco leaf handled well is put into 22 DEG C, and 60% climatic chamber fermentation 48h obtains fermenting tobacco leaf.
3. the carbonization of fermenting tobacco leaf
Fermenting tobacco leaf is put into retort, furnace temperature is gradually heated to about 80 DEG C, keeps 30min, it is dry to fermenting tobacco leaf
It after dry, is brought rapidly up to 160 DEG C, and keep 2h, after inspecting carbonizing degree qualification, closes heat source, take out fermentation carbonization tobacco leaf sample
Product are spare.
4. the preparation of fermentation carbonization tobacco leaf particle
Fermentation carbonization tobacco leaf is taken, its moisture content is detected, supplies moisture content to 10%, being ground into partial size is 120 mesh powders；
By the made tobacco fermentation carbonized powder of 950g, 50g sucrose powder, it is uniformly mixed, is placed in Midi Glatt fluidized bed and is granulated,
Dry 10min under the conditions of the fluidisation air pressure of fluidized bed is 0.15Bar, gas flow temperature is 60 DEG C；Then gelatin 5 is weighed by weight
Part, 2 parts of polyvinylpyrrolidone, 1 part of sodium carboxymethyl starch are configured to the binder solution that concentration is 8% with distilled water, are flowing
Under conditions of change air pressure is 0.2Bar, gas flow temperature is 80 DEG C, atomisation pressure 0.15Bar, hydrojet rate are 10g/min, adopt
Apply 300g binder solution with top spray mode；Adhesive apply after, fluidisation air pressure be 0.15Bar, gas flow temperature is
It is 5 minutes dry under the conditions of 60 DEG C, obtain the fermentation carbonization tobacco leaf particle that moisture content is 8%, rough surface, partial size are 60 mesh.
Embodiment 3
1. Tobacco wears the cultural method of Salmonella L4-6 with embodiment 2.
2. tobacco fermentation
The tobacco leaf 600g for weighing pre-balance moisture to 12% sprays Tobacco and wears Salmonella L4-6 microbial inoculum 120mL, will locate
The tobacco leaf managed is put into 22 DEG C, 60% climatic chamber fermentation 72h.
3. the carbonization of fermenting tobacco leaf
Fermenting tobacco leaf is put into retort, furnace temperature is gradually heated to 80 DEG C, keeps 30min, it is dry to tobacco fermentation sample
It after dry, is brought rapidly up to 130 DEG C, and keep 3h, after inspecting carbonizing degree qualification, closes heat source, take out fermentation carbonization tobacco leaf sample
Product are spare.
4. the preparation of fermentation carbonization tobacco leaf particle
Fermentation carbonization tobacco leaf is taken, its moisture content is detected, supplies moisture content to 8%, being ground into partial size is 140 mesh powders；
By the made tobacco fermentation carbonized powder of 470g, 30g mannitol powder, it is uniformly mixed, is placed in Midi Glatt fluidized bed and is granulated,
Dry 5min under the conditions of fluidized bed fluidisation air pressure is 0.18Bar, gas flow temperature is 60 DEG C；Then gelatin 6 is weighed by weight
Part, 2 parts of polyvinylpyrrolidone, 2 parts of sodium carboxymethyl starch are configured to the binder solution that concentration is 10% with distilled water；?
Under conditions of fluidisation air pressure is 0.2Bar, gas flow temperature is 70 DEG C, atomisation pressure 0.15Bar, hydrojet rate are 12g/ minutes,
200g binder solution is applied using top spray mode；After adhesive has applied, fluidisation air pressure be 0.18Bar, gas flow temperature is
It is 10 minutes dry under the conditions of 60 DEG C, the fermentation carbonization tobacco leaf that moisture content is 10%, rough surface, partial size are 40 mesh is prepared
Particle.
Embodiment 4
To investigate the uniqueness that Tobacco wears Salmonella L4-6 for fermenting tobacco leaf, the difference of embodiment 4 and embodiment 3 exists
In, with Tobacco wear Salmonella L4-6 relationship strain Dyella soli JS12-10 (purchased from Korean agriculture microorganism fungus kind protect
Hiding center (KACC)) replace Tobacco to wear Salmonella L4-6 and ferment to tobacco leaf, remaining operating procedure is obtained with embodiment 3
It is carbonized using the fermentation that moisture content is 10%, rough surface, partial size are 40 mesh obtained by Dyella soli JS12-10 fermentation
Tobacco leaf particle.
Embodiment 5
Fermentation carbonization tobacco leaf particle prepared by embodiment 2 is applied in composite cigarette filter stick, every millimeter of cigarette filter rod adds
Add 1.5mg fermentation carbonization tobacco leaf particle, be prepared into experiment cigarette, the cigarette not add above-mentioned particle carries out sense organ as control group
It smokes panel test and is detected with harmful ingredients in flue gas.Analyses Methods for Sensory Evaluation Results shows: compared with the control group, the cigarette of addition fermentation carbonization tobacco leaf particle
The pungent excitement of branch flue gas throat is substantially reduced, the rich increase of cigarette perfume, and the sweet sense of flue gas is obvious；Harmful ingredients in flue gas detects table
Bright: in experiment cigarette flue gas, phenol reduced rate is 24.3%, illustrates that fermentation carbonization tobacco leaf particle can significantly reduce cigarette cigarette
Phenol content in gas.
Embodiment 6
Fermentation carbonization tobacco leaf particle prepared by embodiment 3 is applied in composite cigarette filter stick, every millimeter of cigarette filter rod adds
Add 1mg fermentation carbonization tobacco leaf particle, be prepared into experiment cigarette, the cigarette not add above-mentioned particle carries out sense organ as control group and comments
It inhales and harmful ingredients in flue gas detects.Analyses Methods for Sensory Evaluation Results shows: compared with the control group, the cigarette of addition fermentation carbonization tobacco leaf particle
Flue gas irritation and bitter pungent are substantially reduced, and natural tobacco aroma is more abundant, and the sweet sense of flue gas is obvious；Harmful ingredients in flue gas inspection
Survey shows: in experiment cigarette flue gas, phenol reduced rate is 22.5%, illustrates that fermentation carbonization tobacco leaf particle can significantly reduce volume
Phenol content in cigarette flue gas.
Embodiment 7
By in embodiment 4, with Dyella soli JS12-10 fermenting tobacco leaf, the fermentation being prepared after further treatment
Carbonization tobacco leaf particle is applied in composite cigarette filter stick, and every millimeter of cigarette filter rod addition 1mg fermentation carbonization tobacco leaf particle is prepared into
Test cigarette；Sensory evaluating smoking and harmful ingredients in flue gas detection are carried out by control group of the experiment cigarette in embodiment 6.Sensory evaluation
The result shows that: compared with the control group, with the experiment cigarette that Dyella soli JS12-10 fermenting tobacco leaf is prepared, cigarette
Without being substantially reduced, the sweet sense of flue gas is insufficient for flue gas irritation and bitter pungent.Harmful ingredients in flue gas detection shows: experiment cigarette flue gas
In, phenol reduced rate is 16.9%, and discovery is compared with data in embodiment 6, and experiment cigarette harmful components reduced rate is not so good as
Control group cigarette.Though it can be seen that being relationship strain, strain Dyella soli JS12-10 is used for tobacco fermentation, can not
It obtains and the Tobacco effect that wear Salmonella L4-6 similar.
As it will be easily appreciated by one skilled in the art that the foregoing is merely illustrative of the preferred embodiments of the present invention, not to
The limitation present invention, any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should all include
Within protection scope of the present invention.
Attached sequence table:
The 16S rDNA partial sequence that Tobacco wears Salmonella L4-6 is as follows:
Sequence table
<110>cigarette industry Co., Ltd in Yunnan
Jasmine Yang Hong Li Yuan high is bright, and how the beautiful Jiao Junsu brave summer is for army building
<120>a kind of fermentation carbonization tobacco leaf particle and its preparation method and application
<130> RIB180325
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 1508
<212> DNA
<213>Tobacco wears Salmonella (Dyellatabacisoli L4-6)
<400> 1
agagtttgat cctggctcag attgaacgct ggcggcatgc ctaacacatg caagtcgaac 60
ggcagcacag cagtagcaat actgtgggtg gcgagtggcg gacgggtgag taatgcatcg 120
ggacctacct agacgtgggg gataacgtag ggaaacttac gctaataccg catacgtcct 180
acgggagaaa gcgggggatc ttcggacctc gcgcggttag acggaccgat gttcgattag 240
ctagttggta gggtaatggc ctaccaaggc gacgatcgat agctggtctg agaggatgat 300
cagccacact ggaactgaga cacggtccag actcctacgg gaggcagcag tggggaatat 360
tggacaatgg gcgcaagcct gatccagcaa tgccgcgtgt gtgaagaagg ccttcgggtt 420
gtaaagcact tttatcagga gcgaaatgcc attggctaat acccggtgga gctgacggta 480
cctgaggaat aagcaccggc taacttcgtg ccagcagccg cggtaatacg aagggtgcaa 540
gcgttaatcg gaattactgg gcgtaaagcg tgcgtaggcg gttcgttagg tccgtcgtga 600
aatccccggg ctcaacctgg gaatggcgat ggatactggc gagctagagt gtgatagagg 660
atggtggaat tcccggtgta gcggtgaaat gcgtagagat cgggaggaac atcagtggcg 720
aaggcggcca tctggatcaa cactgacgct gaggcacgaa agcgtgggga gcaaacagga 780
ttagataccc tggtagtcca cgcccccaaa cgatgcgaac tggatgttgg tctcaactcg 840
gagatcagtg tcgaaagcta acgcgttaag ttcgccgcct ggggagtacg gtcgcaagac 900
tgaaactcaa aggaattgac gggggcccgc acaagcggtg gagtatgtgg tttaattcga 960
tgcaacgcga agaaccttac ctggccttga catgtctgga atcctgcaga gatgcgggag 1020
tgccttcggg aatcagaaca caggtgctgc atggctgtcg tcagctcgtg tcgtgagatg 1080
ttgggttaag tcccgcaacg agcgcaaccc ttgtccttag ttgccagcac gtaatggtgg 1140
gaactctaag gagactgccg gtgacaaacc ggaggaaggt ggggatgacg tcaagtcatc 1200
atggccctta cggccagggc tacacacgta ctacaatggt cggtacagag ggttgcaata 1260
ccgcgaggtg gagccaatcc cagaaagccg atcccagtcc ggatcgtagt ctgcaactcg 1320
actacgtgaa gtcggaatcg ctagtaatcg cggatcagct atgccgcggt gaatacgttc 1380
ccgggccttg tacacaccgc ccgtcacacc atgggagtga gttgctccag aagccgttag 1440
tctaaccgca agggggacga cgaccacgga gtggttcatg actggggtga agtcgtaaca 1500
aggtaacc 1508
Claims (9)
- The tobacco leaf particle 1. a kind of fermentation is carbonized, which is characterized in that it is prepared by method comprising the following steps:(1) Tobacco wears the preparation of Salmonella L4-6 microbial inoculum；(2) Salmonella L4-6 microbial inoculum is worn using Tobacco to ferment to tobacco leaf, obtain fermenting tobacco leaf；(3) fermenting tobacco leaf is subjected to carbonization treatment, obtains fermentation carbonization tobacco leaf；(4) fermentation carbonization tobacco leaf powder is placed on fluidized bed dry, spray adhesive, bond powders formation particle continues to do The dry carbonization tobacco leaf particle of fermentation to obtain the final product.
- The tobacco leaf particle 2. fermentation according to claim 1 is carbonized, which is characterized in that step (1) specifically includes: by tobacco soil Earth is worn Salmonella L4-6 liquid spawn and is inoculated into fermentation medium by 10% inoculum concentration, 28 DEG C shaking flask culture 3~7 days, obtain Culture solution；Medium centrifugal separation, is precipitated with sterile water washing, is finally shaken uniformly with sterile water, and 10 times of dilution up to microbial inoculum.
- The tobacco leaf particle 3. fermentation according to claim 1 is carbonized, which is characterized in that step (2) specifically includes: it weighs in advance The tobacco leaf of equilibrium water conten to 11%~14%, every 100g tobacco leaf spray Tobacco and wear Salmonella L4-6 microbial inoculum 20mL, will handle well Tobacco leaf be placed in 22 DEG C, ferment 24~72h in 60% climatic chamber, obtains fermenting tobacco leaf.
- The tobacco leaf particle 4. fermentation according to claim 1 is carbonized, which is characterized in that step (3) specifically includes: by the cigarette that ferments Leaf is put into retort, and closed heating carbonization treatment is carried out to it, and concrete operation step is that furnace temperature is gradually increased to 80 DEG C, to It is brought rapidly up after fermenting tobacco leaf is dry to 120~300 DEG C, and keeps 1~6h, obtain fermentation carbonization tobacco leaf.
- The tobacco leaf particle 5. fermentation according to claim 1 is carbonized, which is characterized in that step (4) specifically comprises the following steps:1), the moisture content of detection fermentation carbonization tobacco leaf, by moisture control to 8~12%, being ground into partial size is 100~160 mesh Powder；2) 90~99 parts of tobacco leaf powder, 1~10 portion of sucrose or mannitol powder, are weighed according to parts by weight, is uniformly mixed, it is spare；3) 5~10 parts of gelatin, 0~5 part of polyvinylpyrrolidone, 0~2 part of sodium carboxymethyl starch, are weighed according to parts by weight, with steaming Distilled water is configured to the binder solution that concentration is 5~12%；4) it, weighs 300~1000g of mixed-powder in step 2) and is placed in fluidized bed and be granulated, spray adhesive in granulation process Solution is to get fermentation carbonization tobacco leaf particle.
- The tobacco leaf particle 6. fermentation according to claim 5 is carbonized, which is characterized in that step 4) specifically comprises the following steps:(a) mixed-powder is dried to 5~15min under the conditions of fluidizing 0.12~0.2Bar of air pressure, 50~60 DEG C of gas flow temperature；(b) in fluidisation 0.12~0.25Bar of air pressure, 60~80 DEG C of gas flow temperature, 0.08~0.15Bar of atomisation pressure, hydrojet speed Under conditions of 8~12g/min of rate, the prepared binder solution of claim 5 step 3), adhesive are applied using top spray mode Solution usage is the 20%~50% of fermentation carbonization tobacco leaf powder dry material weight；(c) it after adhesive applies, repeats step (a), obtains fermentation carbonization tobacco leaf particle.
- The tobacco leaf particle 7. fermentation according to claim 6 is carbonized, which is characterized in that the fermentation carbonization cigarette that step (c) obtains The moisture content of leaf particle is 8~10%, and partial size is 20~60 mesh, and shape is shaggy similar round particle.
- 8. a kind of cigarette filter tip, which is characterized in that it includes any fermentation carbonization tobacco leaf particles of claim 1-7.
- 9. cigarette filter tip according to claim 8, which is characterized in that add 1~2mg fermentation carbon in every millimeter of cigarette filter tip Change tobacco leaf particle.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811088872.2A CN109315829B (en) | 2018-09-18 | 2018-09-18 | Fermented carbonized tobacco leaf particles and preparation method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811088872.2A CN109315829B (en) | 2018-09-18 | 2018-09-18 | Fermented carbonized tobacco leaf particles and preparation method and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109315829A true CN109315829A (en) | 2019-02-12 |
| CN109315829B CN109315829B (en) | 2021-01-29 |
Family
ID=65265911
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811088872.2A Active CN109315829B (en) | 2018-09-18 | 2018-09-18 | Fermented carbonized tobacco leaf particles and preparation method and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109315829B (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110477437A (en) * | 2019-09-25 | 2019-11-22 | 湖北中烟工业有限责任公司 | A kind of electronic cigarette Chinese style flue-cured tobacco odor type solid particle and preparation method thereof |
| CN113197328A (en) * | 2021-06-09 | 2021-08-03 | 中国农业科学院烟草研究所(中国烟草总公司青州烟草研究所) | Method for reducing using amount of atomizing agent in cigarette without burning by heating |
| CN115537267A (en) * | 2022-09-21 | 2022-12-30 | 云南中烟工业有限责任公司 | Plant spice, preparation method and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105923631A (en) * | 2016-07-13 | 2016-09-07 | 袁春华 | Method for preparing activated carbon adsorbent on basis of nutshells |
| CN106473229A (en) * | 2017-01-04 | 2017-03-08 | 云南中烟工业有限责任公司 | A kind of use fermenting tobacco leaf residue manufactures method and its application of filter stick additive |
-
2018
- 2018-09-18 CN CN201811088872.2A patent/CN109315829B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105923631A (en) * | 2016-07-13 | 2016-09-07 | 袁春华 | Method for preparing activated carbon adsorbent on basis of nutshells |
| CN106473229A (en) * | 2017-01-04 | 2017-03-08 | 云南中烟工业有限责任公司 | A kind of use fermenting tobacco leaf residue manufactures method and its application of filter stick additive |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110477437A (en) * | 2019-09-25 | 2019-11-22 | 湖北中烟工业有限责任公司 | A kind of electronic cigarette Chinese style flue-cured tobacco odor type solid particle and preparation method thereof |
| CN113197328A (en) * | 2021-06-09 | 2021-08-03 | 中国农业科学院烟草研究所(中国烟草总公司青州烟草研究所) | Method for reducing using amount of atomizing agent in cigarette without burning by heating |
| CN113197328B (en) * | 2021-06-09 | 2022-05-31 | 中国农业科学院烟草研究所(中国烟草总公司青州烟草研究所) | Method for reducing using amount of atomizing agent in cigarette without burning by heating |
| CN115537267A (en) * | 2022-09-21 | 2022-12-30 | 云南中烟工业有限责任公司 | Plant spice, preparation method and application thereof |
| CN115537267B (en) * | 2022-09-21 | 2024-03-29 | 云南中烟工业有限责任公司 | Plant spice, preparation method and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN109315829B (en) | 2021-01-29 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN109315829A (en) | A kind of fermentation carbonization tobacco leaf particle and its preparation method and application | |
| CN109722340A (en) | A kind of preparation method and application of open country embankment tobacco aromaticss | |
| CN109749848A (en) | A kind of preparation method and application of a kind of reed mentioned in ancient books chicken tongue grass tobacco aromaticss | |
| CN109722339A (en) | A kind of preparation method and application of fragrant loose tobacco aromaticss | |
| CN109722393A (en) | A kind of tobacco yellow soil source bacterium and its separation method and application | |
| CN114015508A (en) | Spice with high amino acid content, and preparation method and application thereof | |
| CN109288124A (en) | A kind of fermentation carbonization walnut shell particle and its preparation method and application | |
| CN108850267A (en) | A kind of fermentation carbonization Pu'er tea granule and its preparation method and application | |
| CN109315830A (en) | A kind of fermentation carbonization honeysuckle particle and its preparation method and application | |
| CN109722338A (en) | A kind of preparation method and application of Alpinia japonica tobacco aromaticss | |
| CN107151636A (en) | Move application of the coccus in lifting quality of tobacco in cave | |
| CN112998304B (en) | Preparation method and application of baked and fermented gentiana macrophylla spice | |
| CN109722341A (en) | A kind of preparation method and application of Golden flower tobacco aromaticss | |
| CN108220344A (en) | A kind of tobacco aromaticss prepared using microbial fermentation Stauntonia latifolia and its application | |
| CN109288126A (en) | A kind of fermentation carbonization tree moss particle and its preparation method and application | |
| CN109317093A (en) | A kind of fermentation carbonization phoenix tail tea granule and its preparation method and application | |
| CN109136146B (en) | Tobacco soil bacterium, and separation method and application thereof | |
| CN110292203A (en) | A kind of soft quasi- carbonization ferment particulate of plant, preparation method and the usage | |
| CN109749849A (en) | A kind of preparation method and application of fructus piperis longi tobacco aromaticss | |
| CN104178520A (en) | Armeniaca vulgaris fermented extract and preparation method and application thereof | |
| CN108175122A (en) | A kind of method that quality of tobacco is promoted using molten bacillus | |
| CN109222230A (en) | A kind of soft quasi- carbonization ferment particulate of corn Radix Glycyrrhizae blood-snow tea, preparation method and the usage | |
| CN105779307B (en) | A kind of trichoderma aureoviride bacterial strain and the method for preparing Pu'er tea tobacco aromaticss with it | |
| CN114634890A (en) | Azospirillum azotobacter, and separation method and application thereof | |
| CN109549250A (en) | The soft quasi- carbonization ferment particulate of the small blueness mandarin orange of one kind, preparation method and the usage |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |