CN109749849A - A kind of preparation method and application of fructus piperis longi tobacco aromaticss - Google Patents
A kind of preparation method and application of fructus piperis longi tobacco aromaticss Download PDFInfo
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Abstract
The present invention provides a kind of preparation method and application of fructus piperis longi tobacco aromaticss, are prepared by method comprising the following steps: (1) preparation of tobacco yellow soil source bacterium X7X microbial inoculum；(2) it is fermented using tobacco yellow soil source bacterium X7X microbial inoculum to long pepper, obtains fermentation fructus piperis longi；(3) fermentation fructus piperis longi is subjected to carbonization treatment, obtains fermentation carbonization fructus piperis longi；(4) fermentation carbonization fructus piperis longi is impregnated using ethyl alcohol, ultrasonic extraction both obtains fructus piperis longi tobacco aromaticss through filtering and concentrating.The fructus piperis longi tobacco aromaticss are applied in cigarette shreds, while cigarette smoke can be made to have special pungent fragrant and herbal during the smoking process, the slightly unique burnt odor of band significantly enriches cigarette cigarette perfume, and fragrance is soft smooth, flue gas harmony is good, hence it is evident that promotes cigarette smoking quality.
Description
Technical field
The invention belongs to essence spice for cigarette technical fields, and in particular to a kind of preparation method of fructus piperis longi tobacco aromaticss and
Using.
Background technique
Cigarette flavor is the core content of cigarette quality, but along with the development of low coking cigarette, cigarette smoking mouthfeel drop
It is low, fragrance deficiency etc. become tobacco development highlight problem.Perfuming cigarette charging, has been a kind of conventional, required supplement
The technological means of fragrance can make up the deficiency on cigarette tobacco leaf formulation by way of flavoring, charging, eliminate miscellaneous gas, make to roll up
Cigarette cigarette perfume is abundanter, sufficient, to have individual style on perfume (or spice) inhaling taste, smelling, enhances the feature of product.Natural perfume material exists
Play the role of particularly important in cigarette blending, and its application in tobacco blending is also more universal, but with enterprise's cigarette
The quickening of autonomous blending paces, type, quantity, quality, the preparation process etc. of existing natural perfume material have been unable to meet blending skill
The demand of art personnel, so the more spice berry resources of sustainable exploitation utilization, obtain quality more by improving preparation method etc.
It is always that cigarette blending and cigarette product research and develop the real project faced for outstanding natural essence fragrance.
Fructus piperis longi, the nearly mature or mature fruit cluster of the drying for being Piperaceae plant fructus piperis longi Piper longumL..Fructus piperis longi is at home
Main product has warming spleen and stomach for dispelling cold, the function of lower gas analgesic in Indonesia, Philippine, Vietnam in Yunnan, Guangdong, external main product
Effect.Currently, fructus piperis longi as Chinese medicine, is widely used in always treating coldness and pain in the epigastrium, vomiting, diarrhea, cold obstruction causing qi stagnation, the thoracic obstruction heart
Bitterly, it has a headache, the diseases such as toothache, but there is not yet tobacco aromaticss are prepared into applied to the report in cigarette.Volatile oil in fructus piperis longi
Content is larger, but the fructus piperis longi volatile oil for being directly prepared general extraction methods such as steam distillation is applied in cigarette,
Its scorching hot and acrid odors is excessively strong, and flue gas thorn of too choking can be made both to retain after can be by special process processing
Fructus piperis longi special pungent perfume (or spice), while its scorching hot thorn sense of choking is reduced again, keep its fragrance gentle along soft.
The content that tradition extracts a variety of aroma substances in fragrance can be increased using microbial fermentation, it is abundant to promote fragrance
Property, it is added into cigarette, can produce that fragrance is abundant, cigarette of high-quality, meet requirement of the consumer to cigarette quality.
It is fermented using microorganism to itself having odorous plant material, moreover it is possible to which it is former odorous only to be different to assign plant
Special fragrance.Plant carbide while retaining material of vegetable origin part aromatic property, and can assign material of vegetable origin with special
Burnt odor taste.Currently, there is no the correlations for being used to prepare flavors and fragrances using microbial fermentation technology combination carbonization technology to grind
Study carefully.
Summary of the invention
It is an object of the invention to for existing essence spice for cigarette type is not abundant enough and preparation method deficiency etc. is asked
Topic provides a kind of preparation method with special pungent fragrant and herbal note fructus piperis longi tobacco aromaticss and the application in cigarette,
It is to be handled using tobacco yellow soil source bacterium X7X zymotechnique and carbonization technique fructus piperis longi, then pass through solvent extraction process
A kind of fructus piperis longi tobacco aromaticss of good security are obtained, are applied in cigarette shreds, make cigarette smoke that there is special pungent perfume (or spice)
And while herbal, slightly with unique burnt odor, cigarette cigarette perfume can be significantly enriched, and fragrance is soft smooth, flue gas harmony is good
It is good, hence it is evident that promote cigarette smoking quality.
* unless otherwise indicated, percentage employed in the present invention is mass percent.
First aspect present invention is related to a kind of tobacco yellow soil source bacterium X7X, is located away from and picks up from the tobacco life of Kunming one
The tobacco rhizosphere soil sample for producing base has carried out morphology, Physiology and biochemistry property and 16S rRNA sequencing analysis, identification knot to it
Fruit shows that it belongs to yellow soil source Pseudomonas, and microbiological classification is named as tobacco yellow soil source bacterium Flavisolibacter
Sp.X7X, in September in 2018 10 days China Committee for Culture Collection of Microorganisms's common micro-organisms center (referred to as:
CGMCC) preservation, deposit number are CGMCC 16451, and address: the Yard 1, BeiChen xi Road, Chaoyang District, Beijing City Chinese Academy of Sciences is micro-
Biological study institute.
The main morphological features and Physiology and biochemistry property of the isolated tobacco yellow soil source bacterium X7X bacterial strain of the present invention are special
Sign are as follows: the cell of the X7X bacterial strain is Gram-negative, aerobic-type, non-athletic rod-like structure.Cell size is width 0.6-
0.7um, long 1.2-2.0um.After 30 DEG C of R2A agar plate are cultivated 7 days, bacterium colony is presented that yellow, surface be smooth, center projections
Neat in edge, diameter are the shape of 1.0-3.0mm.Bacterial strain can be at 20-40 DEG C, and pH is grown in the range of being 6.0-8.0, most
Suitable growth temperature is 30 DEG C, and optimal pH value is 7.0.The gelatin liquefaction of bacterial strain, oxidizing ferment, catalase, polysorbate40 hydrolysis are anti-
Should result be positive, casein, cellulose, starch and polysorbas20,80 results of hydrolysis are negative.Alkaline phosphatase, the rouge of bacterial strain
Enzyme C4, lipase C8, leucine arylamine enzyme, valine arylamine enzyme, cysteine arylamine enzyme, trypsase, chymotrypsin,
Acid phosphatase, alpha-galactosidase, β-uronic acid enzyme, acid phosphatase testing result are positive, arginine dihydrolase,
The utilization of citric acid, indole reaction, lysine decarboxylase, ornithine decarboxylase, tryptophan deaminase testing result are negative.
The X7X bacterial strain can utilize glyoxalic acid, D-arabitol, formic acid, fructose, N- acetyl-D-galactosamine, sugar
Aldehydic acid, D-ALPHA-Hydroxypropionic acid methyl esters, malic acid and sorbierite cannot utilize aspartic acid, alpha-hydroxybutyric acid, fiber two as carbon source
Sugar, fructose, galactolipin, sucrose, rhamnose and serine.The polarity of the X7X strain cell mainly includes phosphatidyl ethanol
Amine, a unidentified aminophospholipids, two unidentified glycolipids；Main breathing quinone is MK-7；Main fatty acid is
iso-C15:0, iso-C17:03 (C of 3OH and summed feature16:1ω 7c and/or C16:1ω6c)。
The separated obtained tobacco yellow soil source bacterium X7X of the present invention, the G+C content of genome are 49.7%,
For 16S rRNA gene nucleotide series as shown in sequence table, which has submitted international nucleotide sequence database
(GenBank), sequence retrieval number: MG209702.
Second aspect of the present invention is related to a kind of fructus piperis longi tobacco aromaticss, is prepared by method comprising the following steps:
(1) preparation of tobacco yellow soil source bacterium X7X microbial inoculum；
(2) it is fermented using tobacco yellow soil source bacterium X7X microbial inoculum to long pepper, obtains fermentation fructus piperis longi；
(3) fermentation fructus piperis longi is subjected to carbonization treatment, obtains fermentation carbonization fructus piperis longi；
(4) by fermentation carbonization fructus piperis longi using ethyl alcohol immersion and ultrasonic technique assisted extraction, Bi is both obtained through filtering and concentrating
Roots of grass tobacco aromaticss.
Above-mentioned fructus piperis longi tobacco aromaticss, step (1) specifically include: by tobacco yellow soil source bacterium X7X liquid spawn by 10%
Inoculum concentration is inoculated into fermentation medium, 30 DEG C shake culture 7 days, obtain culture solution；Every 1000 mL medium centrifugal separation,
It is precipitated with sterile water washing, finally uniformly with the concussion of 20mL sterile water, 10 times of dilution up to microbial inoculum.
Above-mentioned fructus piperis longi tobacco aromaticss, step (2) specifically include: dry fructus piperis longi plant is ground into 30~80 mesh of partial size
Powder, equilibrium water conten to 10%~13% sprays tobacco yellow soil source bacterium X7X microbial inoculum 20mL in every 100g long pepper, mixes
It is even, the fructus piperis longi powder handled well is placed in 22 DEG C, ferment 24~72h in 60% climatic chamber, obtains fermentation fructus piperis longi.
Above-mentioned fructus piperis longi tobacco aromaticss, step (3) specifically include: fermentation fructus piperis longi being put into retort, is closed to it
Formula heats carbonization treatment, and concrete operation step is that furnace temperature is gradually increased to 80 DEG C, be brought rapidly up after fructus piperis longi of fermenting is dry to
120~300 DEG C, and 1~3h is kept, obtain fermentation carbonization fructus piperis longi.
Above-mentioned fructus piperis longi tobacco aromaticss, step (4) specifically comprise the following steps:
1) ethyl alcohol impregnates: 80~95% concentration ethanols of 8~20 times of fructus piperis longi quality of fermentation carbonization, soak extraction 12 is added
~48h；
2) ultrasonic extraction: the soak in step 1) continues 30~60min of ultrasonic extraction, 500~800w of ultrasonic power；
3) filtering and concentrating: the obtained soak of step 2) is filtered by suction filtration mode, and filtering is not less than 200 mesh；Institute
Obtaining filtrate and being concentrated into density under the conditions of 45~65 DEG C, 60~90kPa is 1.0~1.5g/cm3, obtain fructus piperis longi concentration extraction
Liquid, as fructus piperis longi tobacco aromaticss.
Third aspect present invention is related to a kind of cigarette of fructus piperis longi tobacco aromaticss comprising second aspect of the present invention preparation.
The above-mentioned cigarette comprising fructus piperis longi tobacco aromaticss is by made fructus piperis longi tobacco aromaticss, relative to cigarette shreds quality
0.2~0.5% amount is sprayed on cigarette shreds in spraying mode and is made after diluting 3~5 times of quality with propylene glycol or ethyl alcohol
?.
The invention has the following advantages:
1, the present invention makes full use of endemic plant resource, and fructus piperis longi is prepared into tobacco aromaticss for the first time, is applied to Cigarette processing
In, essence spice for cigarette source and type and cigarette note odor type are enriched, the personalization and diversification of consumer are met
Demand.
2, the present invention carries out fermentation process to fructus piperis longi using novel bacterial tobacco yellow soil source bacterium X7X for the first time, and combination carbonization increases
It after perfume (or spice), carries out solvent extraction again a kind of fructus piperis longi tobacco aromaticss has been made, this kind of preparation process enriches the extraction side of flavouring essence for tobacco
Formula.Compared to the fructus piperis longi volatile oil obtained by general extraction methods such as steam distillation, the fragrance of appearance it is scorching hot stimulation and it is pungent
The problems such as pungent is excessively strong, the fructus piperis longi tobacco aromaticss obtained by preparation method of the present invention had both remained the special pungent perfume (or spice) of fructus piperis longi,
It reduces its scorching hot thorn sense of choking again simultaneously, keeps its fragrance gentle submissive, be applied in cigarette shreds, during the smoking process can
While making cigarette smoke that there is special pungent fragrant and herbal, slightly with unique burnt odor, cigarette cigarette perfume, flue gas association are significantly enriched
Tonality is good, hence it is evident that promotes cigarette smoking quality.
3, the fructus piperis longi tobacco aromaticss prepared using the method for the present invention, entire extraction process are introduced without bad organic solvent,
Gained fragrance and processing technology Environmental Safety, and raw material is easy to get, it is low in cost, it is easy to implement industrialized production, there is good answer
With value.
Detailed description of the invention
Fig. 1 is electromicroscopic photograph of the tobacco yellow soil of the present invention source bacterium X7X on R2A culture medium；
Fig. 2 is tobacco yellow soil of the present invention source bacterium X7X and part related strain according to the building of 16S rRNA gene order
Phylogenetic tree.
Specific embodiment
In order to make the objectives, technical solutions, and advantages of the present invention clearer, with reference to the accompanying drawings and embodiments,
The present invention will be described in further detail.It should be appreciated that specific embodiment described herein is only used to explain this hair
It is bright, it is not intended to limit the present invention.In addition, technology involved in the various embodiments of the present invention described below is special
Sign can be combined with each other as long as they do not conflict with each other.
Embodiment 1
1. separation, culture and the identification of tobacco yellow soil source bacterium X7X
The separation of 1.1 tobacco yellow soil source bacterium X7X
Pedotheque is taken from the tobacco rhizosphere in the tobacco leaf production base of Yunnan Province Kunming, plastic bag sealing is taken back, and 4 DEG C
It saves stand-by.10g soil sample is accurately weighed, 100mL sterile water, 30 DEG C, 200rpm shake culture 30min is added；It will with sterile water
Concentration dilution is to 10-3、10-4W/v times, 0.1mL is respectively taken to be spread evenly across on R2A agar plate the bacterium solution of 2 concentration, each
3 parallel tests are arranged in the bacterium solution of concentration, cultivate 2 weeks in 30 DEG C of constant incubators, the picking different shape list on culture plate
Bacterium colony continues to isolate and purify bacterial strain using plate streak, R2A slant medium is inoculated in after number, or is configured to glycerol and hangs
Liquid (20%, w/v), preservation under the conditions of being placed in -80 DEG C.
Isolated microorganism is inoculated in respectively in R2A fluid nutrient medium, 30 DEG C, 160rpm shaken cultivation, is cultivated
It is OD=1.5 to bacterial concentration, the seed liquor of each microbial strains is made, observes seed liquor appearance and odor characteristic variation, sieve
Select the microbial strains that can wherein generate special aroma, number X7X.
The identification of 1.2 tobacco yellow soil source bacterium X7X
By the above-mentioned microbial strains X7X that can generate special aroma filtered out, continue to be inoculated in the training of R2A agar plate
It supports on base, is cultivated 7 days in 30 DEG C of constant incubators.Carry out morphology, Physiology and biochemistry property and 16S rRNA sequencing analysis, identification
The result shows that belong to yellow soil source Pseudomonas, microbiological classification be named as tobacco yellow soil source bacterium (Latin name:
Flavisolibacter soli.X7X)。
Electromicroscopic photograph of the tobacco yellow soil source bacterium X7X on R2A Agar Plating is as shown in Fig. 1.
The cell of tobacco yellow soil source bacterium X7X bacterial strain is Gram-negative, aerobic-type, non-athletic rod-like structure.Cell
Size is width 0.6-0.7um, long 1.2-2.0um.After 30 DEG C of R2A agar plate are cultivated 7 days, yellow, surface light is presented in bacterium colony
Sliding, center projections neat in edge, diameter are the shape of 1.0-3.0mm.Bacterial strain can be at 20-40 DEG C, and pH is the model of 6.0-8.0
Interior growth is enclosed, optimum growth temperature is 30 DEG C, and optimal pH value is 7.0.The gelatin liquefaction of bacterial strain, oxidizing ferment, catalase,
Polysorbate40 hydrolysis result is positive, and casein, cellulose, starch and polysorbas20,80 results of hydrolysis are negative.Bacterial strain
Alkaline phosphatase, lipase C4, lipase C8, leucine arylamine enzyme, valine arylamine enzyme, cysteine arylamine enzyme, trypsase,
Chymotrypsin, acid phosphatase, alpha-galactosidase, β-uronic acid enzyme, acid phosphatase testing result are positive, essence
The double hydrolases of propylhomoserin, the utilization of citric acid, indole reaction, lysine decarboxylase, ornithine decarboxylase, the inspection of tryptophan deaminase
Result is surveyed to be negative.
The X7X bacterial strain can utilize glyoxalic acid, D-arabitol, formic acid, fructose, N- acetyl-D-galactosamine, sugar
Aldehydic acid, D-ALPHA-Hydroxypropionic acid methyl esters, malic acid and sorbierite cannot utilize aspartic acid, alpha-hydroxybutyric acid, fiber two as carbon source
Sugar, fructose, galactolipin, sucrose, rhamnose and serine.The polarity of the X7X strain cell mainly includes phosphatidyl ethanol
Amine, a unidentified aminophospholipids, two unidentified glycolipids；Main breathing quinone is MK-7；Main fatty acid is
iso-C15:0, iso-C17:03 (C of 3OH and summed feature16:1ω 7c and/or C16:1ω6c)。
The physiological and biochemical property of tobacco yellow soil source bacterium X7X is as shown in table 1:
The biochemical reactions qualification result of 1 tobacco yellow soil source bacterium X7X of table
Note: "+" indicates that result is the positive, and "-" indicates that result is feminine gender
The utilization of carbon source situation of tobacco yellow soil source bacterium X7X is as shown in table 2:
The carbon nitrogen source utilization power of 2 tobacco yellow soil source bacterium X7X of table
Note: "+" indicates that result is the positive, and "-" indicates that result is feminine gender
The 16S rRNA gene nucleotide partial sequence of tobacco yellow soil source bacterium X7X as shown in Detailed description of the invention, the sequence with
Known array in GenBank database carries out BLAST comparative analysis, and the 16S rRNA of related species is obtained from database
Gene nucleotide series, phylogenetic tree construction are shown in attached drawing 2.It is found through comparative analysis, tobacco yellow soil source bacterium of the invention
X7X and bacterial strain (Flavisolibacterswuensis.SR2-4-2) affiliation are nearest, are formed on systematic evolution tree only
Vertical branch, the features such as comprehensive morphological, Physiology and biochemistry, cytochemistry and phylogenetic analysis, the two have obvious gap, table again
Bright tobacco yellow soil source bacterium X7X of the invention is a new species, is named as Flavisolibactersoli.
Tobacco yellow soil source bacterium X7X is in the 16S rRNA gene nucleotide series accession number of GenBank database
MG209702, the deposit number of China Committee for Culture Collection of Microorganisms's common micro-organisms center are CGMCC 16451.
The phylogenetic tree of the 16S rRNA gene order of tobacco yellow soil source bacterium X7X and related species building is as shown in Fig. 2.
Embodiment 2
1. the culture of tobacco yellow soil source bacterium X7X
(1) test tube slant culture
Using slant preservation culture medium, culture medium is R2A agar medium, the formula of culture medium are as follows: glucose 0.5g,
Yeast extract 0.5g, peptone 0.5g, acid hydrolyzed casein 0.5g, soluble starch 0.5g, Sodium Pyruvate 0.3g, phosphoric acid hydrogen two
Potassium 0.3g, magnesium sulfate 0.05g, agar 15g, distilled water constant volume to 1000mL, pH7.2.Culture medium is sterilized 25 at 121 DEG C
Minute, it is put into inclined-plane, tobacco yellow soil source bacterium X7X is inoculated with, is cultivated 2 weeks at 30 DEG C, obtain test tube strains；
(2) seed culture
Using seed culture medium, seed culture medium is R2A fluid nutrient medium, formula are as follows: glucose 0.5g；Yeast extract
0.5g；Peptone 0.5g；Acid hydrolyzed casein 0.5g；Soluble starch 0.5g；Sodium Pyruvate 0.3g；Dipotassium hydrogen phosphate 0.3g；
Magnesium sulfate 0.05g；Distilled water 1000mL, pH7.2.Culture medium is sterilized 25 minutes at 121 DEG C, it is oblique from the test tube of step (1)
On face in picking part mycelium access seed liquor, 30 DEG C of shake culture 48h obtain liquid spawn；
(3) prepared by tobacco yellow soil source bacterium X7X microbial inoculum
Tobacco yellow soil source bacterium X7X liquid spawn is inoculated into fermentation medium by 10% inoculum concentration, 30 DEG C of concussions
Culture 7 days, obtains culture solution；Every 1000mL culture solution is centrifuged 10min with 3500r/min, is precipitated with sterile water washing, finally
Uniformly with the concussion of 20mL sterile water, 10 times of dilution up to tobacco yellow soil source bacterium X7X microbial inoculum.
Fermentative medium formula are as follows: soyabean cake diffusion juice 10mL, glucose 10g, peptone 3g, sodium chloride 2.5g, carbonic acid
Calcium 2g, distilled water 1000mL, PH7.2.Culture medium is sterilized 30min at 121 DEG C, both fermentation medium.
2. fructus piperis longi is fermented
The dry fructus piperis longi fruit ear of 1kg is ground into 50 mesh powder of partial size, equilibrium water conten to 12%, every 100g fructus piperis longi powder
Tobacco yellow soil source bacterium X7X microbial inoculum 20mL is sprayed in end, is mixed, and the fructus piperis longi powder handled well is placed in 22 DEG C, 60% constant temperature is permanent
It ferments in wet tank for 24 hours, obtains fermentation fructus piperis longi.
3. the carbonization for fructus piperis longi of fermenting
Fermentation fructus piperis longi is put into retort, furnace temperature is gradually heated to about 80 DEG C, keeps 30min, fructus piperis longi to be fermented is dry
It after dry, is brought rapidly up to 150 DEG C, and keep 1h, after inspecting carbonizing degree qualification, closes heat source, take out fermentation carbonization fructus piperis longi sample
Product are spare.
4. the preparation of fructus piperis longi tobacco aromaticss
95% concentration ethanol of its 8 times of quality is added into fermentation carbonization fructus piperis longi, soak extraction is for 24 hours；By fermentation carbonization Bi
The soak of the roots of grass continues with ultrasonic extraction instrument assisted extraction 30min, ultrasonic power 600w；Solution after ultrasound is carried out
It filters, filters 200 mesh of mesh number, take filtrate at 45 DEG C, it is 1.3g/cm that density is concentrated to give under the conditions of 70kPa3Fructus piperis longi
Concentrated extracting solution, as fructus piperis longi tobacco aromaticss.
5. the application of fructus piperis longi tobacco aromaticss
The fructus piperis longi tobacco aromaticss for accounting for cigarette shreds quality 0.4% are taken, it is used to the propylene glycol of 5 times of fructus piperis longi tobacco aromaticss amounts
After dissolution, it is sprayed on cigarette shreds, is put into climatic chamber in spraying mode, in humidity 60% ± 2,22 DEG C of temperature
48h is balanced under the conditions of ± 2, rolls into Cigarette, as the test cigarette of the tobacco aromaticss containing fructus piperis longi.
6. the evaluation of fructus piperis longi tobacco aromaticss
It compares the preparation of cigarette: the dry fructus piperis longi fruit ear of 1kg being ground into 50 mesh powder of partial size, by the long pepper
It is carbonized fructus piperis longi instead of fermentation, according to step 4 in embodiment 2,5, the control cigarette containing long pepper extract is made.
The test cigarette of the tobacco aromaticss containing fructus piperis longi, the cigarette that compares with blank cigarette and containing long pepper extract are transferred to specially
The member of smoking of industry carries out sense organ comparison and smokes panel test, and is evaluated referring to flavouring essence for tobacco Evaluation of Functional method, the results are shown in Table 3.
Control cigarette containing long pepper extract, with obvious pungent perfume (or spice), but fragrance relatively stimulates sharply；When addition cigarette is aspirated, increase
Cigarette foreign gas, harmony are bad.The test cigarette of the tobacco aromaticss containing fructus piperis longi has compared with the control cigarette containing long pepper extract
While special pungent fragrant and herbal, fragrance is more smooth soft；When carrying out cigarette smoking, with obvious identification it is pungent it is fragrant,
Herbal and burnt odor, note is naturally soft, the rich increase of flue gas, and flue gas is coordinated, and has been obviously improved cigarette smoking quality.
The sensory evaluation of 3 fructus piperis longi tobacco aromaticss of table
Embodiment 3
1. the cultural method of tobacco yellow soil source bacterium X7X is the same as embodiment 2.
2. fructus piperis longi is fermented
The dry fructus piperis longi fruit ear of 1kg is ground into 80 mesh powder of partial size, equilibrium water conten to 10%, every 100g fructus piperis longi powder
Tobacco yellow soil source bacterium X7X microbial inoculum 20mL is sprayed in end, is mixed, and the fructus piperis longi powder handled well is placed in 22 DEG C, 60% constant temperature is permanent
Ferment 48h in wet tank, obtains fermentation fructus piperis longi.
3. the carbonization for fructus piperis longi of fermenting
Fermentation fructus piperis longi is put into retort, furnace temperature is gradually heated to about 80 DEG C, keeps 40min, fructus piperis longi to be fermented is dry
It after dry, is brought rapidly up to 180 DEG C, and keep 1h, after inspecting carbonizing degree qualification, closes heat source, take out fermentation carbonization fructus piperis longi sample
Product are spare.
4. the preparation of fructus piperis longi tobacco aromaticss
90% concentration ethanol of its 10 times of quality, soak extraction 36h are added into fermentation carbonization fructus piperis longi；Fermentation is carbonized
The soak of fructus piperis longi continues with ultrasonic extraction instrument assisted extraction 40min, ultrasonic power 500w；By the solution after ultrasound into
Row filters, and filters 300 mesh of mesh number, takes filtrate at 50 DEG C, and it is 1.2g/cm that density is concentrated to give under the conditions of 80kPa3Bi
Roots of grass concentrated extracting solution, as fructus piperis longi tobacco aromaticss.
5. the application of fructus piperis longi tobacco aromaticss
The fructus piperis longi tobacco aromaticss for accounting for cigarette shreds quality 0.3% are taken, it are used the 95% of 3 times of fructus piperis longi tobacco aromaticss amounts dense
It after spending ethyl alcohol dissolution, is sprayed on cigarette shreds, is put into climatic chamber in spraying mode, in humidity 60% ± 2, temperature
48h is balanced under the conditions of 22 DEG C ± 2 of degree, rolls into Cigarette, as the test cigarette of the tobacco aromaticss containing fructus piperis longi.
6. the evaluation of fructus piperis longi tobacco aromaticss
Compare the preparation of cigarette: with the relationship strain Flavisolibacterswuensis. of tobacco yellow soil source bacterium X7X
SR2-4-2 (purchased from Japanese Organism Depositary (JCM)) replaces tobacco yellow soil source bacterium X7X fermentation fructus piperis longi, remaining operation step
Suddenly with step 1~5 in embodiment 3, obtained cigarette is to compare cigarette.
The test cigarette that is prepared of fructus piperis longi tobacco aromaticss to be fermented by tobacco yellow soil source bacterium X7X, and blank cigarette,
And the control cigarette being prepared by the long pepper extract that Flavisolibacterswuensis.SR2-4-2 ferments, all hand over
Sense organ comparison is carried out by the member of smoking of profession to smoke panel test, and is evaluated referring to flavouring essence for tobacco Evaluation of Functional method, as a result such as table 4
It is shown.Cigarette is compareed, compared with blank cigarette, there is certain pungent fragrant and burnt odor note, when carrying out cigarette smoking, miscellaneous gas in flue gas,
Irritation increases, and coordinates bad.Cigarette is tested, control cigarette is compared, there is special pungent fragrant and herbal, the pure and fresh hair of fragrance,
Note is coordinated；When carrying out cigarette smoking, pungent fragrant, herbal and burnt odor of the flue gas with obvious identification, the rich increasing of note
Add, aroma quality has more significant promotion, while reducing cigarette smoke irritation.Though it can be seen that being relationship strain, strain
Flavisolibacterswuensis.SR2-4-2 ferments for fructus piperis longi, can not obtain such as tobacco yellow soil source bacterium X7X
Similar effect.
The sensory evaluation of 4 fructus piperis longi tobacco aromaticss of table
Embodiment 4
The step of preparation method of the fructus piperis longi tobacco aromaticss of embodiment 4 and its application method in cigarette are with embodiment 2
1~5.
It compares the preparation of cigarette: after the dry fructus piperis longi fruit ear of 1kg is shredded, after soaking in water 1h, using volatile oil extractor
Routinely extraction by steam distillation 6h, the fraction being collected into obtain volatile oil after being dried over anhydrous sodium sulfate, as fructus piperis longi is volatilized
Oil.Referring to 2 step 5 of embodiment, obtained cigarette is to compare cigarette.
The evaluation of fructus piperis longi tobacco aromaticss: the test cigarette of the tobacco aromaticss containing fructus piperis longi volatilizees with blank cigarette and containing fructus piperis longi
The control cigarette of oil transfers to member of smoking's progress sense organ comparison of profession to smoke panel test, and carries out referring to flavouring essence for tobacco Evaluation of Functional method
Evaluation, the results are shown in Table 5.The control cigarette of the volatile oil containing fructus piperis longi, with obviously pungent perfume (or spice), but the scorching hot stimulation of fragrance, acid
It is excessively strong；When addition cigarette is aspirated, increases flue gas and choke thorn sense obviously, miscellaneous gas increases, and harmony is bad.Containing fructus piperis longi
The test cigarette of tobacco aromaticss, compared with the control cigarette of the volatile oil containing fructus piperis longi, while there is special pungent fragrant and herbal, fragrance
It is more smooth soft；When carrying out cigarette smoking, while cigarette smoke has special pungent fragrant and herbal, the slightly unique burnt odor of band,
Cigarette cigarette perfume is significantly enriched, and fragrance is soft smooth, flue gas harmony is good, hence it is evident that promotes cigarette smoking quality.
The sensory evaluation of 5 fructus piperis longi tobacco aromaticss of table
As it will be easily appreciated by one skilled in the art that the foregoing is merely illustrative of the preferred embodiments of the present invention, not to
The limitation present invention, any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should all wrap
Containing within protection scope of the present invention.
Attached sequence table:
The 16S rRNA gene nucleotide partial sequence of tobacco yellow soil source bacterium X7X is as follows:
Sequence table
<110>cigarette industry Co., Ltd in Yunnan
How beautiful Li Yuandong Gao Lijiaojun Zhou Xingkui Yang Hongming Su Yong is
<120>a kind of preparation method and application of fructus piperis longi tobacco aromaticss
<130> RIB180489
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 1490
<212> DNA
<213>tobacco yellow soil source bacterium (Flavisolibacter soli.X7X)
<400> 1
agagtttgat cctggctcag gatgaacgct agcggcaggc ttaatacatg caagtcgagg 60
ggcagcgcag tgtagcaata catgggcggc gaccggcaaa cgggtgcgga acacgtacgc 120
aacctaccca aaactggggg atagcccacc gaaaggtgga ttaatacctc gtaacctcgt 180
gaagcggcat cgctttatga gtatagctcc ggcggttttg gatgggcgtg cgcctgatta 240
ggtagttggc ggggtaacgg cccaccaagc ctgcgatcag taactggtgt gagagcacga 300
ccagtcacac gggcactgag acacaggccc gactcctacg ggaggcagca gtaaggaata 360
ttggtcaatg gacgcaagtc tgaaccagcc atgccgcgtg agggatgaag gtcctctgga 420
ttgtaaactt cttttatctg ggacgaaacc ctcgttttct aacgggattg acggtaccag 480
aggaataagc accggctaac tccgtgccag cagccgcggt aatacggagg gtgcaagcgt 540
tatccggatt cactgggttt aaagggtgcg taggagggca gggaagtcag tggtgaaatc 600
tccgggctta actcggaaac tgccgttgat actctctgtc ttgaatcccg tggaggtgag 660
cggaatatgt catgtagcgg tgaaatgctt agatatgaca tagaacacca attgcgaagg 720
cagctcgcta cacgaagatt gactctgagg cacgaaagcg tggggatcaa acaggattag 780
ataccctggt agtccacgcc ctaaacgatg gatactcgac atacgcgata cactgtgtgt 840
gtctgagcga aagcattaag tatcccacct gggaagtacg accgcarggt tgaaactcaa 900
aggaattggc gggggtccgc acaagcggtg gagcatgtgg tttaattcga tgatacgcga 960
ggaaccttac ctgggctaga atgctggttg accgtgggtg aaagctcact ttgtagcaat 1020
acacaaccag taaggtgctg catggctgtc gtcagctcgt gccgtgaggt gttgggttaa 1080
gtcccgcaac gagcgcaacc cccatcagta gttgccatca ggtaacgctg ggaactctac 1140
tgaaactgcc gtcgtaagac gcgaggaagg aggggatgat gtcaagtcat catggccttt 1200
atgcccaggg ctacacacgt gctacaatgg ggcgtacaaa gggctgccac ttagcgataa 1260
ggagccaatc ccaaaaaacg cctctcagtt cagatcgcag tctgcaactc gactgcgtga 1320
agctggaatc gctagtaatc gtatatcagc aatgatacgg tgaatacgtt cccggacctt 1380
gcacacaccg cccgtcaagc catggaagct gggtgtacct aaagtcggta accgcaagga 1440
gccgcctagg gtaaaactag taactggggc taagtcgtaa caaggtaacc 1490
Claims (7)
1. a kind of fructus piperis longi tobacco aromaticss, which is characterized in that it is prepared by method comprising the following steps:
(1) preparation of tobacco yellow soil source bacterium X7X microbial inoculum；
(2) it is fermented using tobacco yellow soil source bacterium X7X microbial inoculum to long pepper, obtains fermentation fructus piperis longi；
(3) fermentation fructus piperis longi is subjected to carbonization treatment, obtains fermentation carbonization fructus piperis longi；
(4) fermentation carbonization fructus piperis longi is impregnated using ethyl alcohol, ultrasonic extraction both obtains fructus piperis longi tobacco aromaticss through filtering and concentrating.
2. fructus piperis longi tobacco aromaticss according to claim 1, which is characterized in that step (1) specifically includes: by tobacco yellow soil
Bacterium X7X liquid spawn is inoculated into fermentation medium by 10% inoculum concentration in source, 30 DEG C shake culture 7 days, obtain culture solution；Training
Nutrient solution centrifuge separation, is precipitated with sterile water washing, is finally shaken uniformly with sterile water, and 10 times of dilution up to microbial inoculum.
3. fructus piperis longi tobacco aromaticss according to claim 1, which is characterized in that step (2) specifically includes: by dry fructus piperis longi
Plant is ground into 30~80 mesh powder of partial size, equilibrium water conten to 10%~13%, and tobacco Huang is sprayed in every 100g long pepper
Color soil source bacterium X7X microbial inoculum 20mL is mixed, and the fructus piperis longi powder handled well is placed in 22 DEG C, and fermentation 24 in 60% climatic chamber~
72h obtains fermentation fructus piperis longi.
4. fructus piperis longi tobacco aromaticss according to claim 1, which is characterized in that step (3) specifically includes: fermentation fructus piperis longi is put
Enter in retort, closed heating carbonization treatment is carried out to it, concrete operation step is that furnace temperature is gradually increased to 80 DEG C, wait ferment
It is brought rapidly up after fructus piperis longi is dry to 120~300 DEG C, and keeps 1~3h, obtain fermentation carbonization fructus piperis longi.
5. fructus piperis longi tobacco aromaticss according to claim 1, which is characterized in that step (4) specifically comprises the following steps:
1) ethyl alcohol impregnates: 80~95% concentration ethanols of 8~20 times of fructus piperis longi quality of fermentation carbonization, 12~48h of soak extraction is added；
2) ultrasonic extraction: soak 30~60min of ultrasonic extraction, 500~800w of ultrasonic power in step 1)；
3) filtering and concentrating: the soak that step 2) is obtained filters；Gained filtrate is dense under the conditions of 45~65 DEG C, 60~90kPa
Being reduced to density is 1.0~1.5g/cm3, obtain fructus piperis longi concentrated extracting solution, as fructus piperis longi tobacco aromaticss.
6. a kind of cigarette of tobacco aromaticss containing fructus piperis longi, which is characterized in that it includes any fructus piperis longi cigarette use of claim 1-5
Fragrance.
7. the cigarette of the tobacco aromaticss according to claim 6 containing fructus piperis longi, which is characterized in that will be any in claim 1-5
The fructus piperis longi tobacco aromaticss dilute 3~5 times with propylene glycol or ethyl alcohol with the amount relative to cigarette shreds quality 0.2~0.5%
After quality, it is sprayed on cigarette shreds and is made in spraying mode.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114209086A (en) * | 2021-12-19 | 2022-03-22 | 云南恒罡科技有限公司 | Rosa roxburghii extract, preparation method and application thereof, and low-temperature heating non-combustible product |
| CN114209085A (en) * | 2021-12-19 | 2022-03-22 | 云南恒罡科技有限公司 | Tobacco extract, preparation method and application thereof, and low-temperature heating non-combustible product |
-
2019
- 2019-01-04 CN CN201910007196.XA patent/CN109749849A/en not_active Withdrawn
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114209086A (en) * | 2021-12-19 | 2022-03-22 | 云南恒罡科技有限公司 | Rosa roxburghii extract, preparation method and application thereof, and low-temperature heating non-combustible product |
| CN114209085A (en) * | 2021-12-19 | 2022-03-22 | 云南恒罡科技有限公司 | Tobacco extract, preparation method and application thereof, and low-temperature heating non-combustible product |
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Application publication date: 20190514 |