CN109288126A - A kind of fermentation carbonization tree moss particle and its preparation method and application - Google Patents
A kind of fermentation carbonization tree moss particle and its preparation method and application Download PDFInfo
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- CN109288126A CN109288126A CN201811105975.5A CN201811105975A CN109288126A CN 109288126 A CN109288126 A CN 109288126A CN 201811105975 A CN201811105975 A CN 201811105975A CN 109288126 A CN109288126 A CN 109288126A
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- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24D—CIGARS; CIGARETTES; TOBACCO SMOKE FILTERS; MOUTHPIECES FOR CIGARS OR CIGARETTES; MANUFACTURE OF TOBACCO SMOKE FILTERS OR MOUTHPIECES
- A24D3/00—Tobacco smoke filters, e.g. filter-tips, filtering inserts; Filters specially adapted for simulated smoking devices; Mouthpieces for cigars or cigarettes
- A24D3/06—Use of materials for tobacco smoke filters
- A24D3/14—Use of materials for tobacco smoke filters of organic materials as additive
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- C—CHEMISTRY; METALLURGY
- C01—INORGANIC CHEMISTRY
- C01B—NON-METALLIC ELEMENTS; COMPOUNDS THEREOF; METALLOIDS OR COMPOUNDS THEREOF NOT COVERED BY SUBCLASS C01C
- C01B32/00—Carbon; Compounds thereof
- C01B32/05—Preparation or purification of carbon not covered by groups C01B32/15, C01B32/20, C01B32/25, C01B32/30
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
Abstract
The present invention provides a kind of fermentation carbonization tree moss particles and its preparation method and application, and prepared by method comprising the following steps: (1) Tobacco wears the preparation of Salmonella L4-6 microbial inoculum；(2) Salmonella L4-6 microbial inoculum is worn using Tobacco to ferment to tree moss, obtain fermentation tree moss；(3) fermentation tree moss is subjected to carbonization treatment, obtains fermentation carbonization tree moss；(4) fermentation carbonization tree moss powder is placed on fluidized bed dry, spray adhesive, bond powders formation particle continues to be drying to obtain fermentation carbonization tree moss particle.The particle passes through fermentation process, after being applied to cigarette, can generate the special aroma mutually coordinated with cigarette smoke, enriches cigarette cigarette perfume, improves cigarette odor-absorbing；By carbonization treatment, adsorption specific surface area inside tree moss, suction-operated of the reinforcing particle to cigarette smoke harmful substance can be dramatically increased, while assigning plant granule with special burnt odor taste.
Description
Technical field
The invention belongs to cigarette material technical fields, and in particular to a kind of fermentation carbonization tree moss particle and preparation method thereof and
Using.
Background technique
Cigarette flavor is the core content of cigarette quality, but along with the development of low coking cigarette, cigarette smoking mouthfeel drop
It is low, fragrance deficiency etc. have become tobacco development highlight problem.How to capture influences tobacco aroma quality, the crucial skill of perfume quantity
Art has become the core place for improving production of cigarettes competitiveness.Essence spice for cigarette is added in cigarette shreds, has been a kind of normal
The technological means of rule, required supplement fragrance, but its space for promoting cigarette quality is relatively limited, thus it is non-burning from cigarette
Section is set out, and finds relevant new material to improve, be promoted the fragrance of cigarette, is reduced cigarette smoke irritation and is had become new hair
Exhibition trend.Wherein, some granular materials with porosity and special aroma are added, in cigarette filter rod to improve or be promoted volume
The quality of smoke product is a kind of relatively new, effective research direction.But due in preparation method and raw material selection and group of formula
Face there is also some problems, so that existing granular materials additive advantage fails to give full play of.Such as active carbon, micro mist
Although particle made of the sorptive materials such as silica gel has significant advantage in absorption flue gas harmful substance, perfume (or spice) will also result in simultaneously
A large amount of absorption of taste ingredient, so that cigarette smoking quality significantly reduces.And the plants particles material obtained by granulation, although
The fragrance matter mutually coordinated with cigarette flavor can be introduced into cigarette smoke, but often because the addition of adhesive, causes to plant
Object material hole itself is embedded, and reduces the adsorption site of harmful substance, reduces reducing tar and reducing harm effect.In addition, for original
The selection and processing of material, for the harmony of each plant material and cigarette flavor that the prior art is announced it is still necessary to be promoted, tobacco is fragrant
Gas is abundant not enough, therefore continually looks for the plant material coordinated with tobacco this perfume phase, and need to continue the direction of research.
The content that tradition extracts a variety of aroma substances in fragrance can be increased using microbial fermentation and processing with enzyme preparation, mentioned
It rises that fragrance is rich, and then produces that fragrance is abundant, cigarette of high-quality, meet requirement of the consumer to cigarette quality.It utilizes
Microorganism is fermented to itself having odorous plant material, moreover it is possible to which it is former odorous unique fragrant to be different to assign plant
Taste.
Plant carbide can be such that plant is dehydrated in a short time by high temperature, retain inside plants gap, increase plant to cigarette
While gas noxious substance, not only retain material of vegetable origin part aromatic property, but also assigns material of vegetable origin with special coke
Fragrance.Currently, there is no the correlative studys for utilizing microbial fermentation technology combination carbonization technology to be used for cigarette filter tip particle preparation.
Tree moss, Usneaceae Evernia are that colonize on trunk a kind of is similar to woody fungi plant, main point
The ground such as Yunnan, Guizhou are distributed in, are a kind of important spice berry, natural faint scent and strong pine of the odor characteristic for liverwort
Smell, the essence such as adjustable chypre, lavender, all sorts of flowers, tongue fur perfume (or spice) have preferable fixation to act on.
For disadvantages mentioned above, how to select appropriate material of vegetable origin and be modified, is made with making up prior art technology
At deficiency, reducing, cigarette smoke is irritating simultaneously, improves granular materials and is increasing cigarette smoke harmony and promoting the production of body fluid sense
The effect of aspect etc. becomes the technical issues of needs further solve.
Summary of the invention
The present invention is unobvious to flue gas noxious substance effect for existing plant granule, cannot directly assign to cigarette
Perfume (or spice), or it is bad with cigarette flavor harmony the disadvantages of, a kind of fermentation carbonization tree moss particle and its preparation method and application is provided,
By using Tobacco wear Salmonella L4-6 fermentation and carbonization technique plant material is modified, volume can be enhanced by providing one kind
Cigarette lowering harm and decreasing coking effect reduces cigarette smoke irritation, and can enhance cigarette and assign fragrant effect and promote cigarette smoking quality.
* unless otherwise indicated, percentage employed in the present invention is mass percent.
First aspect present invention is related to a kind of Tobacco and wears Salmonella L4-6, is from Kunming, Yunnan tobacco planting base cigarette
It is isolated in careless pedotheque, morphology, Physiology and biochemistry property and 16S rDNA analysis, qualification result table have been carried out to it
Bright its belongs to Tobacco and wears Salmonella, and microbiological classification is named as Tobacco and wears Salmonella Dyellatabacisoli L4-6,
It is protected on June 19th, 2018 in China Committee for Culture Collection of Microorganisms's common micro-organisms center (referred to as: CGMCC)
Hiding, deposit number are CGMCC 1.16273, address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City Chinese Academy of Sciences microbe research
Institute.
The isolated Tobacco of the present invention wears main morphological features and the Physiology and biochemistry property spy of Salmonella L4-6 bacterial strain
Sign are as follows: the well-grown on most of culture mediums, on R2A agar medium, colony edge is neat, intermediate projections, is in yellow.
Gelatin liquefaction, oxidizing ferment, catalase, polysorbas20,40,60 and 80 hydrolysis are positive, Starch Hydrolysis, cellulose hydrolysis, junket
Proteolysis is negative, and does not generate hydrogen sulfide, does not generate melanin.It can be using maltose, trehalose, cellobiose, lactose, sweet
Reveal sugar, glycerol, aspartic acid, glutamic acid, histidine.The polar lipid ingredient of cell membrane mainly includes phosphatidyl glycerol, double phosphatide
Acyl glycerol and phosphatidyl-ethanolamine, the breathing quinone of cell are CoQ8 (Q-8).
The separated obtained Tobacco of the present invention wears the 16S rDNA gene nucleotide series such as sequence table of Salmonella L4-6
Shown, which has submitted international nucleotide sequence database (GenBank), sequence retrieval number: MF370623.
Second aspect of the present invention is related to a kind of fermentation carbonization tree moss particle, is prepared by method comprising the following steps:
(1) Tobacco wears the preparation of Salmonella L4-6 microbial inoculum；
(2) Salmonella L4-6 microbial inoculum is worn using Tobacco to ferment to tree moss, obtain fermentation tree moss；
(3) fermentation tree moss is subjected to carbonization treatment, obtains fermentation carbonization tree moss；
(4) fermentation carbonization tree moss powder being placed in drying on fluidized bed, spray adhesive, bond powders form particle, after
It is continuous to be drying to obtain fermentation carbonization tree moss particle.
Above-mentioned fermentation is carbonized tree moss particle, and step (1) specifically includes: by Tobacco wear Salmonella L4-6 liquid spawn by
10% inoculum concentration is inoculated into fermentation medium, 28 DEG C shaking flask culture 3~7 days, obtain culture solution；Every 1000mL culture solution from
Heart separation, is precipitated with sterile water washing, and finally uniformly with the concussion of 20mL sterile water, 10 times of dilution up to microbial inoculum.
Above-mentioned fermentation carbonization tree moss particle, step (2) specifically include: weighing the tree of pre-balance moisture to 10%~13%
Tongue fur, every 100g tree moss spray Tobacco and wear Salmonella L4-6 microbial inoculum 20mL, the tree moss handled well are placed in 22 DEG C, 60% constant temperature
Ferment 24~72h in constant humidity cabinet, obtains fermentation tree moss.
Above-mentioned fermentation carbonization tree moss particle, step (3) specifically include: fermentation tree moss being put into retort, is carried out to it
Closed heating carbonization treatment, concrete operation step are that furnace temperature is gradually increased to 80 DEG C, are brought rapidly up after the tree moss that ferments is dry
To 120~300 DEG C, and 1~6h is kept, obtains fermentation carbonization tree moss.
Above-mentioned fermentation carbonization tree moss particle, step (4) specifically comprise the following steps:
1), the moisture content of detection fermentation carbonization tree moss, by moisture control to 8~10%, be ground into partial size be 100~
160 mesh powders；
2) 2~6 parts of gelatin, 3~8 parts of xanthan gum, are weighed according to parts by weight, being configured to concentration with distilled water is 5~10%
Binder solution；
3) the fermentation carbonization 300~1000g of tree moss powder, weighed in step 1) is placed in fluidized bed and is granulated, granulation process
Middle spray adhesive solution is to get fermentation carbonization tree moss particle.
Above-mentioned fermentation carbonization tree moss particle, step 3) specifically comprise the following steps:
(a) fermentation carbonization tree moss powder is done under the conditions of fluidizing 0.12~0.2Bar of air pressure, 50~60 DEG C of gas flow temperature
Dry 5~15min；
(b) in fluidisation 0.12~0.25Bar of air pressure, 60~70 DEG C of gas flow temperature, 0.08~0.15Bar of atomisation pressure, spray
Under conditions of 8~10g/min of liquid speed rate, the prepared binder solution of step 2), binder solution are applied using top spray mode
Dosage is the 20%~40% of fermentation carbonization tree moss powder dry material weight；
(c) it after adhesive applies, repeats step (a), obtains fermentation carbonization tree moss particle.
Above-mentioned fermentation is carbonized tree moss particle, and the moisture content for the fermentation carbonization tree moss particle that step (c) obtains is 10~
12%, partial size is 20~60 mesh, and shape is shaggy similar round particle.
Third aspect present invention is related to a kind of cigarette filter tip, and it includes the fermentation carbonization tree moss of second aspect of the present invention preparation
Particle.
Above-mentioned cigarette filter tip, 1~2mg of addition fermentation carbonization tree moss particle in every millimeter of cigarette filter tip.
The invention has the following advantages:
(1) present invention ferments to it by the way that tree moss is worn Salmonella L4-6 using Tobacco, after being applied to cigarette filter tip,
The special aroma mutually coordinated with cigarette flavor can be generated during cigarette smoking, enriches cigarette cigarette perfume, improves cigarette odor-absorbing；
(2) present invention carries out carbonization treatment by the tree moss after fermenting, and high temperature makes the dehydration in a short time of tree moss cell
And be formed, adsorption specific surface area inside tree moss is increased, fermentation carbonization tree moss particle is significantly improved and the filtering of cigarette smoke is imitated
Rate, can selective absorption cigarette smoke harmful substance, while assign fermentation carbonization tree moss particle with special burnt odor taste；
(3) present invention is not adjusted plant granule production technology, only by the modification to raw material, realizes and improves increasing
Fragrant and tar reduction double effects, raw material are easy to get, low in cost, are easy to implement industrialized production, there is good commercial application value.
Detailed description of the invention
Fig. 1 is that Tobacco of the present invention wears electromicroscopic photograph of the Salmonella L4-6 on R2A culture medium；
Fig. 2 is that Tobacco of the present invention wears Salmonella L4-6 and part related strain according to the building of 16S rDNA gene order
Phylogenetic tree.
Specific embodiment
In order to make the objectives, technical solutions, and advantages of the present invention clearer, with reference to the accompanying drawings and embodiments, right
The present invention is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, and
It is not used in the restriction present invention.As long as in addition, technical characteristic involved in the various embodiments of the present invention described below
Not constituting a conflict with each other can be combined with each other.
Embodiment 1
1. separation, culture and identification that Tobacco wears Salmonella L4-6
1.1 Tobaccos wear the separation of Salmonella L4-6
Tobacco sample is taken from Kunming, Yunnan tobacco planting base, plastic bag sealing is taken back, and 4 DEG C save for use.Accurately
10g soil sample is weighed, 90mL sterile water, 30 DEG C, 180rpm shaking table concussion 30min is added；With sterile water by concentration dilution to 10-1、
10-2、10-3、10-4、10-5Times, take 0.2mL to be coated on LB plating medium respectively the bacterium solution of 5 concentration, each concentration
3 parallel tests are arranged in bacterium solution, cultivate 48h at 30 DEG C, from picking difference single colonie on the plate of every part of soil sample, train in LB plate
It supports and glycerol is added by bacterium solution volume 20% after purification on base, be placed in -80 DEG C of refrigerators and save backup.
Isolated microorganism is inoculated in LB liquid medium, 30 DEG C, 160rpm shaking table shaken cultivation respectively, is trained
Supporting to bacterial concentration is OD=2.0, spare as seed liquor.The seed liquor of each strain to be tested is seeded in screening by 1%
In culture medium, in 30 DEG C, 160rpm shaking table shaken cultivation 3-5 days, fermentation liquid appearance and aroma change situation, screening are observed daily
Obtain one plant of microorganism that can generate fragrance, number L4-6.
1.2 Tobaccos wear the identification of Salmonella L4-6
Morphology, Physiology and biochemistry property and 16S rDNA analysis, qualification result table are carried out to the bacterial strain L4-6 isolated and purified
The bright Tobacco that belongs to wears Salmonella, and microbiological classification is named as Tobacco and wears Salmonella Dyellatabacisoli L4-6.
It is as shown in Fig. 1 that Tobacco wears electromicroscopic photograph of the Salmonella L4-6 on R2A culture medium.
Tobacco wears Salmonella L4-6 well-grown on most of culture mediums, on R2A agar medium, colony edge
Neatly, intermediate projections are in yellow.Gelatin liquefaction, oxidizing ferment, catalase, polysorbas20,40,60 and 80 hydrolysis are positive, form sediment
Powder hydrolysis, cellulose hydrolysis, casein hydrolysis are negative, do not generate hydrogen sulfide, do not generate melanin.Maltose, sea can be utilized
Algae sugar, cellobiose, lactose, mannose, glycerol, aspartic acid, glutamic acid, histidine.The polar lipid ingredient of cell membrane is main
Including phosphatidyl glycerol, cardiolipin and phosphatidyl-ethanolamine, the breathing quinone of cell is CoQ8 (Q-8).
The physiological and biochemical property that Tobacco wears Salmonella L4-6 is as shown in table 1:
1 Tobacco of table wears the physiological and biochemical property of Salmonella L4-6
| Experiment | Growth response | Experiment | Growth response |
| Gelatin liquefaction | +++++ | Polysorbas20,40,60, and 80 | +++++ |
| Starch Hydrolysis | -- | Grown on cellulose | -- |
| Cellulose hydrolysis | -- | Alkaline phosphatase | ++++ |
| Casein hydrolysis | -- | Valine arylamine enzyme | ++++ |
Note: "+" indicates that result is the positive, and "-" indicates that result is feminine gender
The carbon nitrogen utilization power that Tobacco wears Salmonella L4-6 is as shown in table 2:
2 Tobacco of table wears the carbon nitrogen source utilization power of Salmonella L4-6
| Utilization of carbon source | As a result | Carbon source or nitrogen source utilize | As a result |
| Dextrin | + | N- acetyl-d-glucosamine | + |
| Maltose | + | Mannitol | + |
| Trehalose | + | Fructose | + |
| Cellobiose | + | Galactolipin | + |
| Sucrose | - | Rhamnose | - |
| Gossypose | - | Alanine | - |
| Melibiose | + | Histidine | + |
Note: "+" indicates that result is the positive, and "-" indicates that result is feminine gender
Tobacco wears the 16S rDNA partial sequence of Salmonella L4-6 as shown in Detailed description of the invention, the sequence and GenBank number
BLAST comparative analysis is carried out according to the known array in library, and obtains the 16S rDNA gene order of related species, structure from database
Phylogenetic tree is built, sees attached drawing 2.It is found through comparative analysis, Tobacco of the invention wears Salmonella L4-6 and bacterial strain (Dyella
soli JS12-10T) affiliation is nearest, forms independent branch on systematic evolution tree, it is comprehensive morphological, Physiology and biochemistry, thin
The features such as born of the same parents' chemistry and phylogenetic analysis, the two have obvious gap again, show that Tobacco of the invention wears Salmonella L4-6 and is
One new species, is named as Dyellatabacisoli.
Tobacco wears Salmonella L4-6 in the 16S rDNA gene nucleotide series accession number of GenBank database
MF370623, the deposit number of China Committee for Culture Collection of Microorganisms's common micro-organisms center are CGMCC 1.16273.
The phylogenetic tree that Tobacco wears the 16S rDNA gene order building of Salmonella L4-6 and related species is as shown in Fig. 2.
Embodiment 2
1. the culture that Tobacco wears Salmonella L4-6
(1) test tube slant culture
Using slant preservation culture medium, culture medium is R2A agar medium, the formula of culture medium are as follows: glucose 0.5g, ferment
Female cream 0.5g, peptone 0.5g, acid hydrolyzed casein 0.5g, soluble starch 0.5g, Sodium Pyruvate 0.3g, dipotassium hydrogen phosphate
0.3g, magnesium sulfate 0.05g, agar 15g, distilled water constant volume to 1000mL, pH7.2.Culture medium is sterilized 25 points at 121 DEG C
Clock is put into inclined-plane, and inoculation Tobacco is worn Salmonella L4-6 bacterial strain, cultivated 1 week at 28 DEG C, obtains test tube strains；
(2) seed culture
Using seed culture medium, the formula of seed culture medium are as follows: dextrin 120g, soy meal 40g, yeast extract 2g, tryptophan
0.5g, Beta-alanine 5g, magnesium sulfate 0.5g, ammonium phosphate 0.2g, distilled water constant volume to 1000mL, pH7.2.By culture medium 121
It sterilizes 25 minutes, is accessed in seed liquor from picking part mycelium on the test tube slant of step (1), 28 DEG C of shaking flask cultures at DEG C
36h obtains liquid spawn；
(3) Tobacco wears the preparation of Salmonella L4-6 microbial inoculum
Liquid spawn made from step (2) is transferred in fermentation medium by 10% inoculum concentration, 28 DEG C of shaking flask cultures 6
It, gained culture solution is centrifuged 10min with 3500r/min, is precipitated with sterile water washing, and precipitating 20mL sterile water shakes uniformly,
10 times of dilution are that Tobacco wears Salmonella L4-6 microbial inoculum.
Fermentative medium formula are as follows: soy meal 10g, glucose 10g, peptone 3g, sodium chloride 2.5g, calcium carbonate 2g steam
Distilled water constant volume sterilizes 25 minutes to 1000mL, pH7.2,121 DEG C, both obtains culture medium.
2. tree moss ferments
The tree moss 1200g for weighing pre-balance moisture to 12% sprays Tobacco and wears Salmonella L4-6 microbial inoculum 240mL, will
The tree moss handled well is put into 22 DEG C, and 60% climatic chamber fermentation 72h obtains fermentation tree moss.
3. the carbonization for the tree moss that ferments
Fermentation tree moss is put into retort, furnace temperature is gradually heated to about 80 DEG C, keeps 30min, tree moss to be fermented is dry
It after dry, is brought rapidly up to 140 DEG C, and keep 3h, after inspecting carbonizing degree qualification, closes heat source, take out fermentation carbonization tree moss sample
Product are spare.
4. the preparation of fermentation carbonization tree moss particle
Fermentation carbonization tree moss is taken, its moisture content is detected, supplies moisture content to 10%, being ground into partial size is 100 mesh powders；
The made fermentation carbonization tree moss powder of 1000g is placed in Midi Glatt fluidized bed and is granulated, is in the fluidisation air pressure of fluidized bed
0.15Bar, gas flow temperature dry 10min under the conditions of being 50 DEG C；Then 3 parts of gelatin, 5 parts of xanthan gum are weighed by weight, with steaming
Distilled water be configured to concentration be 8% binder solution, fluidisation air pressure be 0.2Bar, gas flow temperature is 60 DEG C, atomisation pressure is
Under conditions of 0.15Bar, hydrojet rate are 10g/min, 250g binder solution is applied using top spray mode；Adhesive has applied
Bi Hou, 10 minutes dry under the conditions of fluidisation air pressure is 0.18Bar, gas flow temperature is 60 DEG C, obtaining moisture content is 11%, table
The fermentation carbonization tree moss particle that face is coarse, partial size is 60 mesh.
Embodiment 3
1. Tobacco wears the cultural method of Salmonella L4-6 with embodiment 2.
2. tree moss ferments
The tree moss 600g for weighing pre-balance moisture to 13% sprays Tobacco and wears Salmonella L4-6 microbial inoculum 120mL, will locate
The tree moss managed is put into 22 DEG C, 60% climatic chamber fermentation 48h.
3. the carbonization for the tree moss that ferments
Fermentation tree moss is put into retort, furnace temperature is gradually heated to 80 DEG C, keeps 45min, it is dry to tree moss fermentation-like
It after dry, is brought rapidly up to 120 DEG C, and keep 4h, after inspecting carbonizing degree qualification, closes heat source, take out fermentation carbonization tree moss sample
Product are spare.
4. the preparation of fermentation carbonization tree moss particle
Fermentation carbonization tree moss is taken, its moisture content is detected, supplies moisture content to 10%, being ground into partial size is 120 mesh powders；
500g fermentation carbonization tree moss powder is placed in Midi Glatt fluidized bed and is granulated, is 0.18Bar, gas in fluidized bed fluidisation air pressure
Flow dry 5min under the conditions of temperature is 60 DEG C；Then 4 parts of gelatin, 6 parts of xanthan gum are weighed by weight, are configured to distilled water dense
The binder solution that degree is 10%；Fluidisation air pressure be 0.2Bar, gas flow temperature is 60 DEG C, atomisation pressure 0.12Bar, hydrojet
Rate is to apply 150g binder solution using top spray mode under conditions of 10g/ minutes；After adhesive has applied, in fluidized gas
5 minutes dry under the conditions of pressure is 0.18Bar, gas flow temperature is 60 DEG C, it is 10%, rough surface, grain that moisture content, which is prepared,
Diameter is the fermentation carbonization tree moss particle of 40 mesh.
Embodiment 4
Uniqueness of the Salmonella L4-6 for the tree moss that ferments is worn to investigate Tobacco, the difference of embodiment 4 and embodiment 3 exists
In, with Tobacco wear Salmonella L4-6 relationship strain Dyella soli JS12-10 (purchased from Korean agriculture microorganism fungus kind protect
Hiding center (KACC)) replace Tobacco to wear Salmonella L4-6 and ferment to tree moss, remaining operating procedure is obtained with embodiment 3
It is carbonized using the fermentation that moisture content is 10%, rough surface, partial size are 40 mesh obtained by Dyella soli JS12-10 fermentation
Tree moss particle.
Embodiment 5
Fermentation carbonization tree moss particle prepared by embodiment 2 is applied in composite cigarette filter stick, every millimeter of cigarette filter rod adds
Add 1mg fermentation carbonization tree moss particle, be prepared into experiment cigarette, the cigarette not add above-mentioned particle carries out sense organ as control group and comments
It inhales and harmful ingredients in flue gas detects.Analyses Methods for Sensory Evaluation Results shows: compared with the control group, the cigarette of addition fermentation carbonization tree moss particle
Flue gas irritation is substantially reduced, and flue gas is soft full, and cigarette odor-absorbing is obviously improved, and has special fragrant；Flue gas nocuousness at
Sorting survey shows: in experiment cigarette flue gas, ammonia reduced rate is 18.8%, crotonaldehyde reduced rate is 20.9%, illustrates the fermentation carbon
Ammonia and crotons aldehyde in cigarette smoke can be significantly reduced by changing tree moss particle.
Embodiment 6
Fermentation carbonization tree moss particle prepared by embodiment 3 is applied in composite cigarette filter stick, every millimeter of cigarette filter rod adds
Add 2mg fermentation carbonization tree moss particle, be prepared into experiment cigarette, the cigarette not add above-mentioned particle carries out sense organ as control group and comments
It inhales and harmful ingredients in flue gas detects.Analyses Methods for Sensory Evaluation Results shows: compared with the control group, the cigarette of addition fermentation carbonization tree moss particle
Flue gas irritation and bitter pungent are substantially reduced, pleasant impression be improved significantly, cigarette perfume aroma quality increases；Harmful ingredients in flue gas inspection
Survey shows: in experiment cigarette flue gas, ammonia reduced rate is 27.9%, crotonaldehyde reduced rate is 24.6%, illustrates fermentation carbonization tree
Tongue fur particle can significantly reduce ammonia and crotons aldehyde in cigarette smoke.
Embodiment 7
By in embodiment 4, fermented tree moss with Dyella soli JS12-10, the fermentation being prepared after further treatment
Carbonization tree moss particle is applied in composite cigarette filter stick, and every millimeter of cigarette filter rod addition 2mg fermentation carbonization tree moss particle is prepared into
Test cigarette；Sensory evaluating smoking and harmful ingredients in flue gas detection are carried out by control group of the experiment cigarette in embodiment 6.Sensory evaluation
The result shows that: compared with the control group, with the experiment cigarette that Dyella soli JS12-10 fermentation tree moss is prepared, cigarette
Without being substantially reduced, pleasant impression improvement property is insufficient, and introduces miscellaneous gas for flue gas irritation and bitter pungent.Harmful ingredients in flue gas detects table
Bright: in experiment cigarette flue gas, ammonia reduced rate is 18.1%, crotonaldehyde reduced rate is 16.5%, is compared with data in embodiment 6
Compared with discovery, tests cigarette harmful components reduced rate and be not so good as control group cigarette.Though it can be seen that being relationship strain, strain
Dyella soli JS12-10 ferments for tree moss, can not obtain and the Tobacco effect that wear Salmonella L4-6 similar.
As it will be easily appreciated by one skilled in the art that the foregoing is merely illustrative of the preferred embodiments of the present invention, not to
The limitation present invention, any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should all include
Within protection scope of the present invention.
Attached sequence table:
The 16S rDNA partial sequence that Tobacco wears Salmonella L4-6 is as follows:
Sequence table
<110>cigarette industry Co., Ltd in Yunnan
The source Gao Lili person is the equal Chen Xing of Wang Jian Zhang Yipeng Li Wen
<120>a kind of fermentation carbonization tree moss particle and its preparation method and application
<130> RIB180320
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 1508
<212> DNA
<213>Tobacco wears Salmonella (Dyellatabacisoli L4-6)
<400> 1
agagtttgat cctggctcag attgaacgct ggcggcatgc ctaacacatg caagtcgaac 60
ggcagcacag cagtagcaat actgtgggtg gcgagtggcg gacgggtgag taatgcatcg 120
ggacctacct agacgtgggg gataacgtag ggaaacttac gctaataccg catacgtcct 180
acgggagaaa gcgggggatc ttcggacctc gcgcggttag acggaccgat gttcgattag 240
ctagttggta gggtaatggc ctaccaaggc gacgatcgat agctggtctg agaggatgat 300
cagccacact ggaactgaga cacggtccag actcctacgg gaggcagcag tggggaatat 360
tggacaatgg gcgcaagcct gatccagcaa tgccgcgtgt gtgaagaagg ccttcgggtt 420
gtaaagcact tttatcagga gcgaaatgcc attggctaat acccggtgga gctgacggta 480
cctgaggaat aagcaccggc taacttcgtg ccagcagccg cggtaatacg aagggtgcaa 540
gcgttaatcg gaattactgg gcgtaaagcg tgcgtaggcg gttcgttagg tccgtcgtga 600
aatccccggg ctcaacctgg gaatggcgat ggatactggc gagctagagt gtgatagagg 660
atggtggaat tcccggtgta gcggtgaaat gcgtagagat cgggaggaac atcagtggcg 720
aaggcggcca tctggatcaa cactgacgct gaggcacgaa agcgtgggga gcaaacagga 780
ttagataccc tggtagtcca cgcccccaaa cgatgcgaac tggatgttgg tctcaactcg 840
gagatcagtg tcgaaagcta acgcgttaag ttcgccgcct ggggagtacg gtcgcaagac 900
tgaaactcaa aggaattgac gggggcccgc acaagcggtg gagtatgtgg tttaattcga 960
tgcaacgcga agaaccttac ctggccttga catgtctgga atcctgcaga gatgcgggag 1020
tgccttcggg aatcagaaca caggtgctgc atggctgtcg tcagctcgtg tcgtgagatg 1080
ttgggttaag tcccgcaacg agcgcaaccc ttgtccttag ttgccagcac gtaatggtgg 1140
gaactctaag gagactgccg gtgacaaacc ggaggaaggt ggggatgacg tcaagtcatc 1200
atggccctta cggccagggc tacacacgta ctacaatggt cggtacagag ggttgcaata 1260
ccgcgaggtg gagccaatcc cagaaagccg atcccagtcc ggatcgtagt ctgcaactcg 1320
actacgtgaa gtcggaatcg ctagtaatcg cggatcagct atgccgcggt gaatacgttc 1380
ccgggccttg tacacaccgc ccgtcacacc atgggagtga gttgctccag aagccgttag 1440
tctaaccgca agggggacga cgaccacgga gtggttcatg actggggtga agtcgtaaca 1500
aggtaacc 1508
Claims (9)
- The tree moss particle 1. a kind of fermentation is carbonized, which is characterized in that it is prepared by method comprising the following steps:(1) Tobacco wears the preparation of Salmonella L4-6 microbial inoculum；(2) Salmonella L4-6 microbial inoculum is worn using Tobacco to ferment to tree moss, obtain fermentation tree moss；(3) fermentation tree moss is subjected to carbonization treatment, obtains fermentation carbonization tree moss；(4) fermentation carbonization tree moss powder is placed on fluidized bed dry, spray adhesive, bond powders formation particle continues to do The dry carbonization tree moss particle of fermentation to obtain the final product.
- The tree moss particle 2. fermentation according to claim 1 is carbonized, which is characterized in that step (1) specifically includes: by tobacco soil Earth is worn Salmonella L4-6 liquid spawn and is inoculated into fermentation medium by 10% inoculum concentration, 28 DEG C shaking flask culture 3~7 days, obtain Culture solution；Medium centrifugal separation, is precipitated with sterile water washing, is finally shaken uniformly with sterile water, and 10 times of dilution up to microbial inoculum.
- The tree moss particle 3. fermentation according to claim 1 is carbonized, which is characterized in that step (2) specifically includes: it weighs in advance The tree moss of equilibrium water conten to 10%~13%, every 100g tree moss spray Tobacco and wear Salmonella L4-6 microbial inoculum 20mL, will handle well Tree moss be placed in 22 DEG C, ferment 24~72h in 60% climatic chamber, obtains fermentation tree moss.
- The tree moss particle 4. fermentation according to claim 1 is carbonized, which is characterized in that step (3) specifically includes: fermentation is set Tongue fur is put into retort, and closed heating carbonization treatment is carried out to it, and concrete operation step is that furnace temperature is gradually increased to 80 DEG C, to It is brought rapidly up after fermentation tree moss is dry to 120~300 DEG C, and keeps 1~6h, obtain fermentation carbonization tree moss.
- The tree moss particle 5. fermentation according to claim 1 is carbonized, which is characterized in that step (4) specifically comprises the following steps:1), the moisture content of detection fermentation carbonization tree moss, by moisture control to 8~10%, being ground into partial size is 100~160 mesh Powder；2) 2~6 parts of gelatin, 3~8 parts of xanthan gum, are weighed according to parts by weight, with distilled water be configured to concentration be 5~10% it is viscous Mixture solution；3) the fermentation carbonization 300~1000g of tree moss powder, weighed in step 1) is placed in fluidized bed and is granulated, and sprays in granulation process Binder solution is spilt to get fermentation carbonization tree moss particle.
- The tree moss particle 6. fermentation according to claim 5 is carbonized, which is characterized in that step 3) specifically comprises the following steps:(a) will fermentation carbonization tree moss powder under the conditions of fluidize 0.12~0.2Bar of air pressure, 50~60 DEG C of gas flow temperature dry 5~ 15min；(b) in fluidisation 0.12~0.25Bar of air pressure, 60~70 DEG C of gas flow temperature, 0.08~0.15Bar of atomisation pressure, hydrojet speed Under conditions of 8~10g/min of rate, the prepared binder solution of claim 5 step 2), adhesive are applied using top spray mode Solution usage is the 20%~40% of fermentation carbonization tree moss powder dry material weight；(c) it after adhesive applies, repeats step (a), obtains fermentation carbonization tree moss particle.
- The tree moss particle 7. fermentation according to claim 6 is carbonized, which is characterized in that the fermentation that step (c) obtains, which is carbonized, sets The moisture content of tongue fur particle is 10~12%, and partial size is 20~60 mesh, and shape is shaggy similar round particle.
- 8. a kind of cigarette filter tip, which is characterized in that it includes any fermentation carbonization tree moss particles of claim 1-7.
- 9. cigarette filter tip according to claim 8, which is characterized in that add 1~2mg fermentation carbon in every millimeter of cigarette filter tip Change tree moss particle.
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| CN115537267A (en) * | 2022-09-21 | 2022-12-30 | 云南中烟工业有限责任公司 | Plant spice, preparation method and application thereof |
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| CN101828769A (en) * | 2010-04-16 | 2010-09-15 | 云南正邦生物技术有限公司 | Preparation method of cigarette flavor compensating particles |
| CN104178522A (en) * | 2014-08-19 | 2014-12-03 | 云南中烟工业有限责任公司 | Evernia furfuracea fermented extract and preparation method and application thereof |
| CN105923631A (en) * | 2016-07-13 | 2016-09-07 | 袁春华 | Method for preparing activated carbon adsorbent on basis of nutshells |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101828769A (en) * | 2010-04-16 | 2010-09-15 | 云南正邦生物技术有限公司 | Preparation method of cigarette flavor compensating particles |
| CN104178522A (en) * | 2014-08-19 | 2014-12-03 | 云南中烟工业有限责任公司 | Evernia furfuracea fermented extract and preparation method and application thereof |
| CN105923631A (en) * | 2016-07-13 | 2016-09-07 | 袁春华 | Method for preparing activated carbon adsorbent on basis of nutshells |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115537267A (en) * | 2022-09-21 | 2022-12-30 | 云南中烟工业有限责任公司 | Plant spice, preparation method and application thereof |
| CN115537267B (en) * | 2022-09-21 | 2024-03-29 | 云南中烟工业有限责任公司 | Plant spice, preparation method and application thereof |
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