CN106957724A - A kind of method that immobilization acetic acid bacteria prepares cigarette Fructus Rubi extract - Google Patents
A kind of method that immobilization acetic acid bacteria prepares cigarette Fructus Rubi extract Download PDFInfo
- Publication number
- CN106957724A CN106957724A CN201610899646.7A CN201610899646A CN106957724A CN 106957724 A CN106957724 A CN 106957724A CN 201610899646 A CN201610899646 A CN 201610899646A CN 106957724 A CN106957724 A CN 106957724A
- Authority
- CN
- China
- Prior art keywords
- acetic acid
- acid bacteria
- solution
- fructus rubi
- extract
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B9/00—Essential oils; Perfumes
- C11B9/02—Recovery or refining of essential oils from raw materials
-
- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
- A24B15/18—Treatment of tobacco products or tobacco substitutes
- A24B15/24—Treatment of tobacco products or tobacco substitutes by extraction; Tobacco extracts
- A24B15/241—Extraction of specific substances
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/10—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a carbohydrate
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Mycology (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Molecular Biology (AREA)
- Fats And Perfumes (AREA)
Abstract
The present invention provides a kind of method that immobilization acetic acid bacteria prepares cigarette Fructus Rubi extract.The preparation method is concretely comprised the following steps：(1) water extraction at 95~100 DEG C of raspberry dry fruit is taken repeatedly, to merge extract solution, obtain Fructus Rubi extract A；(2) wet thallus is obtained after being inoculated into broth bouillon culture centrifugation using acetic acid bacteria, in the mixed solution that wet thallus is added to polyvinyl alcohol and sodium alginate, and with containing 1~3%CaCl2Boric acid saturated solution and saturation Na2SO4Immobilization acetic acid bacteria bead is crosslinked to obtain in solution；(3) by Fructus Rubi extract A and immobilization acetic acid bacteria bead according to mass ratio 5~10:1 mixing after fermentation, then filters to obtain clarified broth, and alcoholic solution is added after freeze-drying and precipitates, filter, be concentrated to give Fructus Rubi extract.The present invention, which is added in cigarette, enriches cigarette perfume, and increase raspberry feature note and the fragrant note of acid, soft fine and smooth flue gas increase flue gas conglobation property, increase flue gas concentration, reduces excitant, improve throat's comfortableness.
Description
Technical field
The present invention relates to cigarette additive field, and in particular to prepares cigarette Fructus Rubi extract using immobilization acetic acid bacteria
Preparation method.
Background technology
Flavoring and casing is the key link in cigarette process, is the determinant and raising cigarette flavor for improving product style
One of effective means of quality.Current essence spice for cigarette is main or passes through the extraction to natural animal-plant, chemical synthesis system
It is standby.Due to by natural plant resource is limited and the harsher limitation of chemical synthesis condition.With the development of biotechnology, in recent years
Come, prepare cigarette flavor using microbe fermentation method, an economy, efficient approach are provided for the production of cigarette flavor,
Develop the new cigarette flavor that some conventional methods can not be produced.
Immobilized microorganism technology, is to be fixed on special microorganism on the carrier of choosing card, makes its highly dense and protect
Bioactivity is held, the biotechnology that can quickly, largely breed under condition of compatibility can make up suspension microorganism fermentation process
The shortcomings of efficiency is low, separation of solid and liquid is difficult, mithridatism is poor.Therefore, in recent years, the research of immobilized microorganism technology is lived very much
Jump, development is very fast, has application to many industrial departments such as food fermentation industry, environmental protection, new energy development and biological biography
Sensor etc., and deep development is achieved, show powerful superiority.It is mainly concerned with the following aspects：In food hair
It is used to produce ethanol, beer, Yoghourt etc. in ferment industry；It is used for environmental monitoring and the processing of industrial wastewater in environmental protection；
It is used to produce hydrogen, methane, biochemical fuel cell, microorganism battery etc. in new energy development；Immobilized microorganism is alternatively arranged as molecule
Recognition component, for making various biology sensors.And it is continuous ripe, perfect with fixation of microbe technology, gradually to height
The direction such as effect low energy consumption, low cost, simplification, safety automation, pollution-free is developed, dirty in energy crisis, shortage of resources, environment
In the case that dye is on the rise, it thus will be promoted more deeply and to be widely applied in terms of wastewater treatment, with more deepening
Remote meaning.Especially in terms of wastewater treatment, have great application prospect.But preparing cigarette using immobilized microorganism technology
Report is but had no with Fructus Rubi extract.
Raspberry (scientific name：Rubus chingii Hu) be rose family rubus plant dry fruit, be distributed in river
The ground such as Soviet Union, Zhejiang, Anhui, Fujian, Jiangxi, Guangxi.With supplementing the kidney to control the nocturnal contracting urine, the effect of nourishing the liver to improve visual acuity is usually used in treatment and lost
Smart involuntary emission, enuresis frequent micturition, impotence and premature ejaculation, mesh is secretly dim-sighted etc..Raspberry medicinal extract by raw material of raspberry, Fructus Rubi extract exist
It is widely used in cigarette, with abundant cigarette perfume, soft fine and smooth flue gas, increase flue gas concentration, the irritating effect of reduction.
Acetic acid bacteria is that a class can produce the common name of organic acetic bacterials such as a large amount of acetic acid, acetic acid using fermentable carbohydrate
Bacterium is widely used in the brewing industries such as vinegar, fruit vinegar, but the seldom report in the preparation of cigarette natural perfume material.Acetic acid bacteria exists
A large amount of organic acids are produced in fermentation process, these organic acids have the work of reduction excitant and soft fine and smooth flue gas in cigarette
With simultaneously, moreover it is possible to assign the sour fragrant breeze lattice of cigarette feature, therefore, acetic acid bacteria fermentation is in the quality side of lifting cigarette natural perfume material
Face has a good application prospect.
The content of the invention
It is an object of the invention to provide a kind of preparation method of cigarette cigarette Fructus Rubi extract, using immobilization acetic acid bacteria
Fermentation technique, develops a kind of new Fructus Rubi extract, and the extract can be used in cigarette, can enrich cigarette perfume,
Cigarette flavor quality is lifted, increases the cigarette flavor property sent out thoroughly, soft fine and smooth flue gas reduces excitant, improves throat's comfortableness, cover
Miscellaneous gas is covered, improves pleasant impression
The present invention provides a kind of method that immobilization acetic acid bacteria prepares cigarette Fructus Rubi extract, it is characterised in that specific bag
Include following steps：
(1) raspberry dry fruit is taken, the water of 2~6 times of its weight is added, 12-24h, the refluxing extraction at 95~100 DEG C is soaked
2~3 times, each extraction time is 5~8 hours, and room temperature is cooled to after having extracted, and is filtrated to get extract solution, and merging is repeatedly carried
Liquid is taken, Fructus Rubi extract A is obtained；
(2) acetic acid bacteria is inoculated into broth bouillon, cultivated under conditions of 37 DEG C of temperature, speed of agitator are 50rpm
48 hours, take nutrient solution in being centrifuged under 8000rpm, the wet thallus after centrifugation is standby；
(3) polyvinyl alcohol-Immobilization in Sodium Alginate of acetic acid bacteria：Using polyvinyl alcohol water solution and sodium alginate aqueous solution
The mixed solution of the polyvinyl alcohol containing mass percent 6%~10% and the sodium alginate of mass percent 1%~3% is prepared,
Then the acetic acid bacteria wet thallus prepared in the step of the 2~5% of total solution volume are added into mixed solution (2), is configured to mix
Liquid is closed, the mixed liquor prepared is added dropwise to CaCl by the syringe needle in 1~5mm apertures with peristaltic pump2Mass percent
For in 1~3% boric acid saturated solution, then bead is taken out and adds saturation Na by crosslinking 1h formation beads2SO4Handed in solution
Join 3-5h, obtain PVA-SA immobilization acetic acid bacteria beads, finally using aseptic water washing, dry for standby；
(4) by the PVA-SA immobilization acetic acid bacteria beads of drying in Fructus Rubi extract A and step (3) according to mass ratio 5
~10:1 mixing, temperature be 30~37 DEG C, speed of agitator be 1~5rpm under conditions of ferment 2~4d, be subsequently placed in 4 DEG C it is cold
7~14d is stood in cabinet；
(5) step (4) fermentation after fermentation liquid is filtered, obtains clarified broth, and zymotic fluid is freeze-dried, its is cold
Lyophilized dry feed liquid thickness is 1~2 centimetre, and vacuum is 10Pa, and drying time is 6~12h；
(6) zymotic fluid after step (5) is freeze-dried, adds 80~95% alcoholic solutions of 2~4 times of its quality, sinks
Form sediment 24~48 hours, it is 0.1MPa to filter to take supernatant in pressure, and temperature is to be concentrated into relative density under conditions of 45 DEG C to be
1.10~1.15, obtain Fructus Rubi extract.
The acetic acid bacteria used in the present invention is acetobacter aceti (Acetobacter aceti) PDH2.05, in 2016 8
It is preserved within 29th Wuhan University's China typical culture collection center the moon, preserving number is CCTCC NO：M 2016436.
The immobilized microorganism technology that the present invention is used has that fermentation process efficiency high, separation of solid and liquid be easy, mithridatism is strong
The advantages of, the deficiency of traditional biological technique can be made up.
The present invention, using immobilization acetic acid bacteria fermentation Fructus Rubi extract, obtains a kind of quality using raspberry as raw material
Excellent cigarette Fructus Rubi extract, the extract has abundant cigarette perfume in cigarette, increase raspberry feature note, soft thin
The effects such as greasy flue gas, increase flue gas conglobation property, increase flue gas concentration, reduction excitant, improvement throat comfortableness, moreover it is possible to assign volume
The sour fragrant breeze lattice of cigarette characteristic.Compared with existing raspberry medicinal extract and Fructus Rubi extract, the Fructus Rubi extract drops in cigarette
Low sharp property and the effect of soft fine and smooth flue gas are more apparent, while also assigning the sour fragrant breeze lattice of cigarette characteristic；Sent out with suspension acetic acid bacteria
Ferment is compared, and Fructus Rubi extract organic acid content prepared by this method is added in 4.32%~9.65%, cigarette effect assessment,
Oral cavity pleasant impression is more comfortable, and fragrance more enriches.This method instead of the traditional extractive technique of Fructus Rubi extract, have updated life
Thing fermentation preparation technology so that it applicability can increase in cigarette, and the preparation to cigarette Fructus Rubi extract provides one
Bar new way.
Embodiment
With reference to embodiment to further detailed description of the present invention.
The acetic acid bacteria used in the present invention is acetobacter aceti (Acetobacter aceti) PDH2.05, in 2016 8
It is preserved within 29th Wuhan University's China typical culture collection center the moon, preserving number is CCTCC NO：M 2016436.
PVA-SA (polyvinyl alcohol-sodium alginate) immobilization acetic acid bacteria bead in following examples of the present invention be according to
Lower method is obtained using polyvinyl alcohol (PVA) and sodium alginate (SA) Dichlorodiphenyl Acetate being fixed of bacterium：
(1) acetic acid bacteria is inoculated into broth bouillon, cultivated under conditions of 37 DEG C of temperature, speed of agitator are 50rpm
48 hours, take nutrient solution in being centrifuged under 8000rpm, the wet thallus after centrifugation is standby；Broth bouillon is conventional in the above method
Broth medium, its Ju Ti Pei Fang be (every liter)：Peptone 10g, beef extract powder 3g, sodium chloride 5g, final pH 7.3 ±
0.1；
(2) polyvinyl alcohol-Immobilization in Sodium Alginate of acetic acid bacteria：Using polyvinyl alcohol water solution and sodium alginate aqueous solution
The mixed solution of the polyvinyl alcohol of the 6%-10% containing mass percent and mass percent 1%-3% sodium alginate is prepared, so
The acetic acid bacteria wet thallus prepared in the step of adding the 2-5% of total solution volume in backward mixed solution (2), is configured to mixing
Liquid, CaCl is added dropwise to peristaltic pump by the syringe needle in 1-5mm apertures by the mixed liquor prepared2Mass percent be 1-
In 3% boric acid saturated solution, then bead is taken out and adds saturation Na by crosslinking 1h formation beads2SO43- is crosslinked in solution
5h, obtains PVA-SA immobilization acetic acid bacteria beads, finally using aseptic water washing, dry for standby；It is described to contain 1-3%CaCl2's
The amount and saturation Na of boric acid saturated solution2SO4The amount of solution has no particular/special requirement, contains 1-3%CaCl2Boric acid saturated solution
Only need to that mixed liquor can be made to produce precipitation, meet the immobilization requirement of acetic acid bacteria, Na2SO4Solution is only needed to bead
It is completely soaked and just may be used；The boric acid saturated solution and saturation Na2SO4Solution is saturated aqueous solution.
Embodiment 1, a kind of method that immobilization acetic acid bacteria prepares cigarette Fructus Rubi extract, comprises the following steps：
(1) water that its weight adds 5 times is added into raspberry dry fruit, refluxing extraction 2 times at 100 DEG C are extracted every time
Time is 5 hours, and room temperature is cooled to after having extracted, and is filtrated to get extract solution, merges extract solution twice, obtains raspberry extraction
Thing A, the condition extracted twice is identical；
(2) the PVA-SA immobilization acetic acid bacteria beads for preparing Fructus Rubi extract A and the above method are according to mass ratio 5:1
Mixing, is 30 DEG C in temperature, speed of agitator is the 4d that fermented under conditions of 5rpm, is subsequently placed in 4 DEG C of refrigerators and preserves 10d；
(3) step (2) fermentation after fermentation liquid is filtered, obtains clarified broth, and the zymotic fluid after filtering is freezed
Dry, feed liquid thickness is 1~2 centimetre, and vacuum is 10Pa, and drying time is 8h；
(4) zymotic fluid after step (3) is freeze-dried, adds 95% alcoholic solution of 3 times of its quality, and precipitation 24 is small
When, it is 0.1MPa to filter to take supernatant in pressure, and temperature is that to be concentrated into relative density under conditions of 45 DEG C be 1.15, obtains raspberry
Extract.
The Fructus Rubi extract prepared in embodiment 1 be added in cigarette enrich cigarette perfume, increase raspberry feature note and
Sour perfume note, soft fine and smooth flue gas increases flue gas conglobation property, increases flue gas concentration, reduces excitant, improves throat's comfortableness.
Embodiment 2, a kind of method that immobilization acetic acid bacteria prepares cigarette Fructus Rubi extract, comprises the following steps：
(1) raspberry dry fruit is taken, the water of 6 times of its weight is added, refluxing extraction 3 times at 95 DEG C, each extraction time is 7
Hour, room temperature is cooled to after having extracted, and extract solution is filtrated to get, merge No. three extract solutions, obtain Fructus Rubi extract A；Three
The condition of secondary extraction is identical；
(2) the PVA-SA immobilization acetic acid bacteria beads that will be prepared in Fructus Rubi extract A and the fixation above method prepared
According to mass ratio 8:1 mixing, is 35 DEG C in temperature, speed of agitator is the 3d that fermented under conditions of 1rpm, is subsequently placed in 4 DEG C of refrigerators
Preserve 8d；
(3) step (2) fermentation after fermentation liquid is filtered, obtains clarified broth, and the zymotic fluid after filtering is freezed
Dry, feed liquid thickness is 1~2 centimetre, and vacuum is 10Pa, and drying time is 10h；
(4) by dried zymotic fluid in step (3), the alcoholic solution of concentration 80% of 4 times of its quality is added, precipitation 48 is small
When, it is 0.1MPa to filter to take supernatant in pressure, and temperature is that to be concentrated into relative density under conditions of 45 DEG C be 1.10, obtains raspberry
Extract.
The Fructus Rubi extract prepared in embodiment 2 be added in cigarette enrich cigarette perfume, increase raspberry feature note and
Sour perfume note, soft fine and smooth flue gas increases flue gas conglobation property, increases flue gas concentration, reduces excitant, improves throat's comfortableness.
Embodiment 3, a kind of method that immobilization acetic acid bacteria prepares cigarette Fructus Rubi extract, comprises the following steps：
(1) raspberry is taken, the water of 3 times of its weight is added, refluxing extraction 3 times at 95 DEG C, each extraction time is 8 small
When, room temperature is cooled to after having extracted, and extract solution is filtrated to get, merge No. three extract solutions, obtain Fructus Rubi extract A；Three times
Extraction conditions are identical；
(2) the PVA-SA immobilization acetic acid bacteria beads for preparing Fructus Rubi extract A and the above method are according to mass ratio 10:
1 mixing, is 37 DEG C in temperature, speed of agitator is the 2d that fermented under conditions of 4rpm, is subsequently placed in 4 DEG C of refrigerators and preserves 7d；
(3) step (2) fermentation after fermentation liquid is filtered, obtains clarified broth, and the zymotic fluid after filtering is freezed
Dry, feed liquid thickness is 1~2 centimetre, and vacuum is 10Pa, and drying time is 6h；
(4) by step (3) dried zymotic fluid, the alcoholic solution of concentration 95% of 2 times of its quality is added, precipitation 48 is small
When, it is 0.1MPa to filter to take supernatant in pressure, and temperature is that to be concentrated into relative density under conditions of 45 DEG C be 1.15, obtains raspberry
Extract.
The Fructus Rubi extract prepared in embodiment 3 be added in cigarette enrich cigarette perfume, increase raspberry feature note and
Sour perfume note, soft fine and smooth flue gas increases flue gas conglobation property, increases flue gas concentration, reduces excitant, improves throat's comfortableness.
Contrast experiment, the yellow spring that the chamomile extract that applicant prepares the present invention is prepared with existing traditional extraction process
Chrysanthemum extract, the chamomile extract prepared that fermented using suspension acetic acid bacteria are added in pipe tobacco according to normal perfuming mode, are made
Into cigarette, and smoked panel test, its effect of smokeing panel test is as follows：
The sensory evaluation effect that raspberry prepared by three kinds of different process is extracted in cigarette compares
It can be seen that to come from above-mentioned contrast experiment, the chamomile extract for preparing is added in cigarette effect more in the present invention
It is good, and found according to specific experiment analysis, compared with suspension acetic acid bacteria ferments, Fructus Rubi extract prepared by this method is organic
Acid content is added in 4.32%~9.65%, cigarette effect assessment, and oral cavity pleasant impression is more comfortable, and fragrance more enriches.
Claims (2)
1. a kind of method that immobilization acetic acid bacteria prepares cigarette Fructus Rubi extract, it is characterised in that specifically include following steps：
(1) raspberry dry fruit is taken, the water of 2~6 times of its weight is added, 12-24h, the refluxing extraction 2~3 at 95~100 DEG C is soaked
Secondary, each extraction time is 5~8 hours, and room temperature is cooled to after having extracted, and is filtrated to get extract solution, merges multiple extract solution,
Obtain Fructus Rubi extract A；
(2) acetic acid bacteria is inoculated into broth bouillon, culture 48 is small under conditions of 37 DEG C of temperature, speed of agitator are 50rpm
When, take nutrient solution in being centrifuged under 8000rpm, the wet thallus after centrifugation is standby；
(3) polyvinyl alcohol-Immobilization in Sodium Alginate of acetic acid bacteria：Prepared using polyvinyl alcohol water solution and sodium alginate aqueous solution
The mixed solution of the sodium alginate of polyvinyl alcohol and mass percent 1%~3% containing mass percent 6%~10%, then
The acetic acid bacteria wet thallus prepared in the step of the 2~5% of total solution volume are added into mixed solution (2), is configured to mixed liquor,
The mixed liquor prepared is added dropwise to CaCl by the syringe needle in 1~5mm apertures with peristaltic pump2Mass percent for 1~
In 3% boric acid saturated solution, then bead is taken out and adds saturation Na by crosslinking 1h formation beads2SO43- is crosslinked in solution
5h, obtains PVA-SA immobilization acetic acid bacteria beads, finally using aseptic water washing, dry for standby；
(4) by the Fructus Rubi extract A prepared in step (1) and the PVA-SA immobilization acetic acid bacteria beads of drying in step (3)
According to mass ratio 5~10:1 mixing, is 30~37 DEG C in temperature, and speed of agitator is 2~4d of fermentation under conditions of 1~5rpm, so
After be placed in 4 DEG C of refrigerators 7~14d of preservation；
(5) filtering fermentation liquor after step (4) fermentation refrigeration is taken, clarified broth is obtained, and zymotic fluid is freeze-dried, its
The feed liquid thickness of freeze-drying is 1~2 centimetre, and vacuum is 10Pa, and drying time is 6~12h；
(6) zymotic fluid after step (5) freeze-drying is taken, 80~95% alcoholic solutions of 2~4 times of its quality, precipitation 24 are added
~48 hours, it was 0.1MPa to filter to take supernatant in pressure, temperature be concentrated under conditions of 45 DEG C relative density be 1.10~
1.15, obtain Fructus Rubi extract.
2. the method that a kind of immobilization acetic acid bacteria according to claim 1 prepares cigarette Fructus Rubi extract, its feature exists
In：Acetic acid bacteria in the step (2) is acetobacter aceti (Acetobacter aceti) PDH2.05, and deposit number is
CCTCC NO：M 2016436.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610899646.7A CN106957724A (en) | 2016-10-13 | 2016-10-13 | A kind of method that immobilization acetic acid bacteria prepares cigarette Fructus Rubi extract |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610899646.7A CN106957724A (en) | 2016-10-13 | 2016-10-13 | A kind of method that immobilization acetic acid bacteria prepares cigarette Fructus Rubi extract |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106957724A true CN106957724A (en) | 2017-07-18 |
Family
ID=59481496
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610899646.7A Pending CN106957724A (en) | 2016-10-13 | 2016-10-13 | A kind of method that immobilization acetic acid bacteria prepares cigarette Fructus Rubi extract |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106957724A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114854594A (en) * | 2022-05-06 | 2022-08-05 | 江苏科技大学 | Preparation method of edible vinegar composite direct-vat-set strain protective agent |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR930001381B1 (en) * | 1990-09-01 | 1993-02-27 | 오뚜기식품 주식회사 | Process for the preparation of vinegar by immobilised microorganism |
| CN102987545A (en) * | 2012-12-18 | 2013-03-27 | 湖北中烟工业有限责任公司 | Preparation method of raspberry fermenting substance for cigarette |
-
2016
- 2016-10-13 CN CN201610899646.7A patent/CN106957724A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR930001381B1 (en) * | 1990-09-01 | 1993-02-27 | 오뚜기식품 주식회사 | Process for the preparation of vinegar by immobilised microorganism |
| CN102987545A (en) * | 2012-12-18 | 2013-03-27 | 湖北中烟工业有限责任公司 | Preparation method of raspberry fermenting substance for cigarette |
Non-Patent Citations (1)
| Title |
|---|
| 王克明: "PVA固定化细胞发酵灵芝醋酸饮料的研究", 《中国酿造》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114854594A (en) * | 2022-05-06 | 2022-08-05 | 江苏科技大学 | Preparation method of edible vinegar composite direct-vat-set strain protective agent |
| CN114854594B (en) * | 2022-05-06 | 2023-09-22 | 江苏科技大学 | Preparation method of edible vinegar composite direct-vat-set strain protective agent |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102406231B (en) | Fermented rose extract for cigarettes and preparation method thereof | |
| CN107604011B (en) | Method for improving anaerobic fermentation gas production by using carbon quantum dots as accelerant | |
| CN106497906A (en) | A kind of preparation method of cigarette chamomile extract | |
| CN102119631A (en) | Grifola frondosa strain for producing polysaccharide with composite raw material of rice bran and wheat bran | |
| CN104388496A (en) | Method for producing N-acetylglucosamine by degrading chitin with enzyme method | |
| CN102796716A (en) | Method for preparing tannase | |
| CN107509915B (en) | Method for reducing cyanide content of cassava residue | |
| CN103589759B (en) | A kind of waste molasses and the two carbon source through fermentation of sewer oil produce the method for sophorolipid | |
| CN106957839A (en) | A kind of method that immobilized yeast prepares cigarette Flos Rosae Rugosae extract | |
| CN106957837A (en) | A kind of method that fixing lactic acid bacteria prepares cigarette Semen Persicae extract | |
| CN106957724A (en) | A kind of method that immobilization acetic acid bacteria prepares cigarette Fructus Rubi extract | |
| CN106957861A (en) | A kind of method that immobilized yeast prepares cigarette violet extract | |
| CN102154370B (en) | Method for preparing flavor for elsholtzia kachinensis prain tobacco through multi-strain composite fermentation | |
| CN106957866A (en) | A kind of immobilized yeast prepares method of the cigarette with red bayberry extract | |
| CN106957873A (en) | A kind of fixing lactic acid bacteria prepares method of the cigarette with Acacia extract | |
| CN106957872A (en) | A kind of method that fixing lactic acid bacteria prepares cigarette Semen Armeniacae Amarum extract | |
| CN107674886A (en) | A kind of immobilization acetic acid bacteria prepares method of the cigarette with windproof root extract | |
| CN106957838A (en) | A kind of method that immobilization acetic acid bacteria prepares cigarette iris extract | |
| CN105733962B (en) | A kind of trichoderma aureoviride HLMM-04 bacterial strain and the method for preparing tobacco aromaticss | |
| CN107201383A (en) | It is a kind of to improve the D-ALPHA-Hydroxypropionic acid production method that D-ALPHA-Hydroxypropionic acid produces intensity | |
| CN107668768A (en) | A kind of method that fixing lactic acid bacteria prepares cigarette angelica extract | |
| CN106957863A (en) | A kind of method that immobilized yeast prepares cigarette black cloth woods extract | |
| CN106957865A (en) | A kind of method that immobilized yeast prepares cigarette apricot extract | |
| CN106957862A (en) | A kind of immobilized yeast prepares method of the cigarette with flores aurantii extract | |
| CN106957871A (en) | A kind of immobilization acetic acid bacteria prepares method of the cigarette with bay extract |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170718 |