CN113040410B - Method for moisturizing cigar wrapper tobacco leaves - Google Patents
Method for moisturizing cigar wrapper tobacco leaves Download PDFInfo
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- CN113040410B CN113040410B CN201911370016.0A CN201911370016A CN113040410B CN 113040410 B CN113040410 B CN 113040410B CN 201911370016 A CN201911370016 A CN 201911370016A CN 113040410 B CN113040410 B CN 113040410B
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- tobacco leaves
- cigar
- wrapper tobacco
- pullulan
- cigar wrapper
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- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B3/00—Preparing tobacco in the factory
- A24B3/02—Humidifying packed raw tobacco
Abstract
A method for moisturizing cigar wrapper tobacco leaves belongs to the technical field of cigar tobacco leaf preservation. In order to improve the moisturizing effect of the cigar wrapper tobacco leaves, further expand the storage environment range of the cigars in a short time and reduce the irreparable loss of the cigar wrappers caused by the sudden change of environmental conditions, the invention provides a method for moisturizing the cigar wrapper tobacco leaves, which comprises the following steps: spraying or smearing water solution of pullulan on cigar outer wrapper tobacco leaves, then placing the tobacco leaves in an environment with the temperature of 23-27 ℃ and the relative humidity of 50% -60% for 4-6h for moisturizing, wherein the concentration of the water solution of the pullulan is 0.8-1.2g/L. The invention can widen the storage environment range of the cigars in a short time, reduce the irreparable loss of the cigar coat caused by the sudden change of environmental conditions and play an important role in the cigar maintenance.
Description
Technical Field
The invention belongs to the technical field of cigar tobacco preservation, and particularly relates to a method for moisturizing cigar wrapper tobacco.
Background
The cigar is a special tobacco product, generally comprises three parts of a cigar coat (outer wrapper tobacco leaf), a cigar sleeve (inner wrapper tobacco leaf) and a cigar core (core tobacco leaf), wherein the outer wrapper tobacco leaf is the only visible part and is essence of the cigar, so that the cigar not only needs to have typical fragrance matched with the cigar, but also has higher requirements on quality factors such as toughness, tissue structure, oil content, color and the like, particularly, the cigar coat is directly contacted with the environment, and the environmental change directly has important influence on the moisture of the cigar coat. Especially in northern areas, the environmental humidity is low, which is an examination for the quality of cigars and for the eggplant clothes, and the eggplant clothes are easy to burst when the moisture is low. In the maintenance and preservation of the cigars, professional maintenance is provided for the cigars by using professional equipment, which is a very effective method, but the high investment of the professional maintenance equipment is difficult to achieve by general people; in addition, the metal aluminum pipe is a common mode, the cigar humidity is kept as much as possible by utilizing a closed environment, and the moisturizing effect of the mode is not obvious. Therefore, the method which is relatively effective, relatively low in price and simple to operate is found to be of great significance for the moisture preservation of the eggplant clothes.
Disclosure of Invention
In order to improve the moisturizing effect of the cigar wrapper tobacco leaves, further expand the storage environment range of the cigars in a short time and reduce the irreparable loss of the cigar wrappers caused by the sudden change of environmental conditions, the invention provides a method for moisturizing the cigar wrapper tobacco leaves, which comprises the following steps: spraying or coating a water solution of pullulan on cigar outer wrapper tobacco leaves, then putting the tobacco leaves in an environment with the temperature of 23-27 ℃ and the relative humidity of 50-60% for 4-6h for moisturizing, wherein the concentration of the water solution of the pullulan is 0.8-1.2g/L.
Further, before spraying or smearing the water solution of the pullulan, the cigar outer wrapper tobacco leaves are pretreated as follows: placing the cigar wrapper tobacco leaves at the same position in an environment with the temperature of 25-30 ℃ and the relative humidity of 55% -65%, standing for 4-5h, and then spreading the cigar wrapper tobacco leaves.
Further defined, before spraying or painting the aqueous solution of pullulan, the cigar wrapper tobacco leaves are pretreated as follows: placing cigar wrapper tobacco leaves at the same part in an environment with 27 ℃ and 60% relative humidity, standing for 4.5h, and then spreading to spread the cigar wrapper tobacco leaves.
Further limited, the pullulan polysaccharide is prepared by fermenting aureobasidium pullulans (Aureobasidium pullulans) UVMU6-1, and the preservation number of the strain of the aureobasidium pullulans (Aureobasidium pullulans) UVMU6-1 is CGMCC NO.13178.
Further limited, the preparation method of the pullulan comprises the following steps: inoculating Aureobasidium pullulans (Aureobasidium pullulans) UVMU6-1 seeds into a YPD culture medium at 28-32 ℃, culturing at 150-200rpm to obtain first-stage seeds, inoculating the obtained first-stage seeds into a shake flask of the YPD culture medium according to the inoculation proportion of 5-10% of the volume of the fermentation culture medium to culture to obtain second-stage seeds, and then inoculating the second-stage seed liquid into the fermentation culture medium to ferment to obtain the pullulan polysaccharide.
Further defined, the secondary seed liquid OD 600 Is 10-15.
Advantageous effects
The extracellular water-soluble mucopolysaccharide pullulan produced by fermentation of aureobasidium pullulans has good elasticity, gas barrier property, plasticity, viscosity, film-forming property and lower oxygen permeability, is easy to dissolve in water, is non-toxic, harmless, colorless and tasteless, can be widely applied to the fields of medicine, food, light industry, chemical industry, petroleum and the like, and has no bad smell during combustion.
The method disclosed by the invention has the advantages that the pullulan polysaccharide is utilized to moisturize the cigar wrapper tobacco leaves, the method is simple, the storage environment range of the cigar in a short time is further expanded, the irretrievable loss of the cigar wrapper tobacco leaves caused by environmental condition mutation is reduced, and the method is expected to play an important role in cigar maintenance.
Detailed Description
The present invention will be described in further detail with reference to examples.
It will be appreciated by those skilled in the art that the following examples are illustrative of the invention only and should not be taken as limiting the scope of the invention.
Fermentation medium: 3 to 4 percent of glucose, 0.8 to 1.2 percent of yeast powder, 1.5 to 2.5 percent of peptone, 0.4 to 0.6 percent of dipotassium hydrogen phosphate, 0.03 to 0.06 percent of magnesium sulfate, 0.03 to 006 percent of ammonium sulfate and 0.05 to 0.1 percent of sodium chloride, wherein the contents are mass contents.
YPD medium: 0.8-1.2% of yeast powder, 1.5-2.5% of peptone and 1.5-2.5% of glucose, wherein the contents are mass contents.
Aureobasidium pullulans UVMU6-1, which is deposited in China general microbiological culture Collection center in 2016, 10, 24 days, with the address of Beijing City republic of south China quarter, xilu No.1, institute of microbiology, institute of Chinese academy of sciences, strain deposit number: CGMCC NO.13178.
Example 1: a method for moisturizing cigar wrapper tobacco leaves by using pullulan polysaccharide.
The method comprises the following specific steps:
step (1), preparing a cigar wrapper tobacco sample: placing the cigar wrapper tobacco leaves at the same part in a constant temperature and humidity box, setting the conditions to be 25 ℃,55% of relative humidity, standing for 5 hours, after the tobacco leaves are fully uniform, dampening, carrying out sheet spreading treatment, fully and flatly spreading the cigar wrapper tobacco leaves, and ensuring that the water content of the cigar wrapper tobacco leaves is uniform between a treatment group and a control group;
step (2), preparing a humectant for the cigar wrapper tobacco leaves: preparing 0.8g/L aqueous solution of pullulan produced by fermenting aureobasidium pullulans, and fully and uniformly dissolving to obtain a treatment solution; the method for producing the pullulan polysaccharide by fermenting the aureobasidium pullulans comprises the following steps:
inoculating Aureobasidium pullulans UVMU6-1 (CGMCC NO. 13178) seed into YPD culture medium, culturing at 28 deg.C and 150rpm to obtain first-stage seed, inoculating the first-stage seed into YPD culture medium according to 5% of culture medium volume to obtain second-stage seed, and inoculating the second-stage seed (OD) to obtain second-stage seed 600 10) inoculating the solution into a fermentation medium, fermenting at 28 deg.C and 150rpm for 169h to obtain pullulan.
And (3) specifically using the humectant for the cigar wrapper tobacco leaves: specifically, a layer of the humectant prepared in the step (2) is sprayed on the surface of the cigar wrapper tobacco leaves, so that the humectant is uniformly sprayed, and the comparison treatment is water treatment with the same operation.
And (4) verifying the tobacco leaf moisturizing effect of the cigar wrapper: after the cigar wrapper tobacco leaves are sprayed or smeared, the treatment group and the control group are placed in an environment with the temperature of 23 ℃ and the relative humidity of 50%, the water content of the cigar wrapper tobacco leaves between the treatment group and the control group is respectively measured after 4 hours, and the moisturizing effect of the treatment group is verified. The moisture-keeping effect of the cigar wrapper tobacco leaves means that the moisture content of the cigar wrapper tobacco leaves in the treatment group using the humectant is obviously higher than that of the cigar wrapper tobacco leaves in the control group.
Example 2: a method for moisturizing cigar wrapper tobacco leaves by using pullulan polysaccharide.
The method comprises the following specific steps:
step (1), preparing a cigar wrapper tobacco sample: placing the cigar wrapper tobacco leaves at the same part in a constant temperature and humidity box, setting the conditions to be 27 ℃,60% relative humidity, standing for 4.5 hours, after the tobacco leaves are fully and uniformly moistened, carrying out spreading treatment, fully and flatly spreading the cigar wrapper tobacco leaves, and ensuring that the water content of the cigar wrapper tobacco leaves is uniform between a treatment group and a control group;
step (2), preparing a humectant for the cigar wrapper tobacco leaves: preparing 1.0g/L solution of pullulan produced by fermenting aureobasidium pullulans, and fully and uniformly dissolving to obtain a treatment solution; the method for producing the pullulan polysaccharide by fermenting the aureobasidium pullulans comprises the following specific steps:
inoculating Aureobasidium pullulans UVMU6-1 (CGMCC NO. 13178) seed into YPD culture medium at 30 deg.C and 175rpm to obtain first-stage seed, inoculating the obtained first-stage seed into YPD culture medium according to 7.5% of culture medium volume to obtain second-stage seed, and inoculating second-stage seed solution (OD) 600 12) inoculating into a fermentation medium, fermenting at 28 deg.C and 150rpm for 20h to obtain pullulan.
And (3) specifically using the humectant for the cigar wrapper tobacco leaves: specifically, the humectant prepared in the step (2) is smeared on the surface of the cigar wrapper tobacco leaves, so that the humectant is uniformly smeared, and the contrast treatment is water treatment in the same operation.
And (4) verifying the moisture-keeping effect of the cigar wrapper tobacco leaves: after the cigar wrapper tobacco leaves are sprayed or smeared, the treatment group and the control group are placed in an environment with the temperature of 25 ℃ and the relative humidity of 55 percent, the water content of the cigar wrapper tobacco leaves between the treatment group and the control group is respectively measured after 5 hours, and the moisturizing effect of the treatment group is verified. The moisture-keeping effect of the cigar wrapper tobacco leaves means that the moisture content of the cigar wrapper tobacco leaves in the treatment group using the humectant is obviously higher than that of the cigar wrapper tobacco leaves in the control group.
Example 3: a method for moisturizing cigar wrapper tobacco leaves by using pullulan polysaccharide.
The method comprises the following specific steps:
step (1), preparing a cigar wrapper tobacco sample: placing the cigar wrapper tobacco leaves at the same part in a constant temperature and humidity box, setting the conditions to be 30 ℃,65% relative humidity, standing for 4 hours, regaining moisture after the tobacco leaves are fully uniform, carrying out spreading treatment, fully and flatly spreading the cigar wrapper tobacco leaves, and ensuring that the water content of the cigar wrapper tobacco leaves is uniform between a treatment group and a control group;
step (2), preparing a humectant for the cigar wrapper tobacco leaves: preparing 1.2g/L solution of pullulan produced by fermenting aureobasidium pullulans, and fully and uniformly dissolving to obtain a treatment solution; the method for producing the pullulan polysaccharide by fermenting the aureobasidium pullulans comprises the following steps:
inoculating aureobasidium pullulans UVMU6-1 (CGMCC NO. 13178) seeds into a YPD culture medium at 32 ℃, culturing at 200rpm to obtain first-stage seeds, inoculating the obtained first-stage seeds into the YPD culture medium according to 10% of the volume of the culture medium to obtain second-stage seeds, and then inoculating (OD) second-stage seed liquid to obtain 600 15) fermenting the obtained product into a fermentation medium at 28 ℃,150rpm and 24h to obtain the pullulan polysaccharide.
And (3) specifically using the humectant for the cigar wrapper tobacco leaves: specifically, a layer of the humectant prepared in the step (2) is sprayed on the surface of the cigar wrapper tobacco leaves, so that the humectant is uniformly sprayed, and the comparison treatment is water treatment with the same operation.
And (4) verifying the moisture-keeping effect of the cigar wrapper tobacco leaves: after smearing the cigar wrapper tobacco leaves, placing the treatment group and the comparison group in an environment with the temperature of 27 ℃ and the relative humidity of 60%, respectively measuring the water content of the cigar wrapper tobacco leaves between the treatment group and the comparison group after 6h, and verifying the moisturizing effect of the treatment group. The moisture-keeping effect of the cigar wrapper tobacco leaves means that the moisture content of the cigar wrapper tobacco leaves in the treatment group using the humectant is obviously higher than that of the cigar wrapper tobacco leaves in the control group.
The water content of the cigar wrapper tobacco leaves after being moisturized by the method is detected, and the results are shown in the following table:
TABLE 1 Water content test results
| Group of | Water content (%) |
| Example 1 | 16.72±2.73％ |
| Control of example 1 | 16.19±1.54％ |
| Example 2 | 19.63±3.42％ |
| Control of example 2 | 16.72±1.33％ |
| Example 3 | 21.03±3.78％ |
| Control of example 3 | 19.61±3.22％ |
The results show that: under experimental conditions, the water content of the tobacco leaves in the cigar wrapper of the embodiment is higher than that of the tobacco leaves in the cigar wrapper of the control group by using the pullulan, so that the pullulan has a certain moisturizing effect on the cigar wrapper tobacco leaves.
Although the present invention has been described with reference to the preferred embodiments, it should be understood that various changes and modifications can be made therein by those skilled in the art without departing from the spirit and scope of the invention as defined in the appended claims.
Claims (5)
1. A method for moisturizing cigar wrapper tobacco leaves is characterized by comprising the following steps: spraying or coating a water solution of pullulan on the pretreated cigar outer wrapper tobacco leaves, and then putting the tobacco leaves in an environment with the temperature of 25-27 ℃ and the relative humidity of 55-60% for 5-6h for moisturizing, wherein the concentration of the water solution of the pullulan is 1.0-1.2 g/L; the pretreatment is to place the cigar wrapper tobacco leaves at the same part in an environment with the temperature of 25-30 ℃ and the relative humidity of 55-65%, stand for 4-5h, and then unfold the cigar wrapper tobacco leaves.
2. The method of claim 1, wherein the pretreatment comprises leaving the same portion of the cigar wrapper tobacco leaves at 27 ℃ under 60% relative humidity for 4.5 hours, and then spreading the cigar wrapper tobacco leaves.
3. The method according to claim 1, wherein the pullulan is prepared from Aureobasidium pullulans (Aureobasidium pullulans) (A), (B), or (C)Aureobasidium pullulans)UVMU6-1 is prepared by fermentation, and the Aureobasidium pullulans (A), (B) and (C)Aureobasidium pullulans)The preservation number of the UVMU6-1 strain is CGMCC NO.13178.
4. The method according to claim 3, wherein the pullulan is prepared by a process comprisingThe method comprises the following steps: aureobasidium pullulans (A), (B), (C)Aureobasidium pullulans)Inoculating UVMU6-1 seeds into YPD culture medium at 28-32 deg.C, culturing at 150-200rpm to obtain first-stage seeds, inoculating the obtained first-stage seeds into shaking flask of YPD culture medium according to inoculation ratio of 5-10% of fermentation culture medium volume, culturing to obtain second-stage seeds, and inoculating the second-stage seed liquid into fermentation culture medium for fermentation to obtain pullulan.
5. The method of claim 4, wherein the secondary seed fluid OD is 600 Is 10-15.
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Citations (8)
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2019
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| CN107373743A (en) * | 2017-08-21 | 2017-11-24 | 安徽皖南烟叶有限责任公司 | A kind of junior tobacco leaf conditioning device and moisture regain method |
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