CN108812671A - A method of rice is improved to rice blast disease resistance using alginic acid oligosaccharides - Google Patents
A method of rice is improved to rice blast disease resistance using alginic acid oligosaccharides Download PDFInfo
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- CN108812671A CN108812671A CN201810878160.4A CN201810878160A CN108812671A CN 108812671 A CN108812671 A CN 108812671A CN 201810878160 A CN201810878160 A CN 201810878160A CN 108812671 A CN108812671 A CN 108812671A
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- rice
- alginic acid
- disease resistance
- acid oligosaccharides
- oligosaccharides
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
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- Life Sciences & Earth Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
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- General Health & Medical Sciences (AREA)
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Abstract
The present invention provides a kind of method using alginic acid oligosaccharides raising rice to rice blast disease resistance.Alginic acid oligosaccharides is sprayed by the rice seedling phase, improves defensive ferment --- the activity of phenylalanine lyase, catalase, peroxidase in rice leaf, and then enhances rice to the disease resistance of rice blast.Using method of the invention, paddy disease-resistant ability is improved, promotes the growth of rice.Meanwhile this method is simple and easy, is conducive to agricultural extension application.
Description
Technical field
It is the invention belongs to field of agricultural production technologies, in particular to a kind of to be improved using alginic acid oligosaccharides sprinkling rice seedling
New method of the rice to rice blast disease resistance.
Background technique
Rice is the important cereal crops in China, in national plantation extensively.And rice blast is that China's rice region endangers most serious
One of rice disease, have different degrees of generation every year, will cause rice yield decline, quality reduces.At present to rice blast
The prevention and treatment of disease is mainly Agro-chemicals control.However in recent years, chemical pesticide is frequently arbitrarily applied without distinction
The problems such as having caused serious ecological environmental pollution and medicament residue is badly in need of developing pollution-free degradable biological pesticide.Oligosaccharides
Have many advantages, such as it is harmless to the mankind and ecological environment, be not likely to produce drug resistance, be biodegradable, it has also become the development of biological pesticide
Direction.Different from chemical pesticide, oligosaccharides acts not only as antibacterial agent and directly kills pathogenic microorganism, can also be planted by induction
Object defence response is to resist plant disease.Therefore, bioactive oligosaccharide can excite the immunocompetence of plant to resist microorganism
Disease infects, and achievees the purpose that the generation for preventing crops microorganism disease.
Alginic acid be formed by beta-D-mannuronic acid and α-L- guluronic acid by β -1,4 glucosides key connection it is linear
Anion polysaccharide, also referred to as algin can be made into water-soluble good alginic acid oligosaccharides by certain method.
Alginic acid abundance, oligosaccharides production cost is low, nontoxic, nuisanceless, fully meets energy-saving and environment-friendly health idea, meets society
Growth requirement.Meanwhile alginic acid oligosaccharides have various biological activity, such as antitumor, neuroprotection, promote plant growth,
It is antibacterial etc., there is huge application value on many fields such as new drug development, health food.The present invention adds in the rice seedling phase
Add alginic acid oligosaccharides to carry out the generation of inducing paddy rice defensive ferment, improve rice to the disease resistance of rice blast, promote paddy growth,
Using meeting food safety and environmental requirement.
Summary of the invention
To solve prior art problem, present invention is primarily aimed at provide a kind of utilization alginic acid oligosaccharides raising rice pair
The method of rice blast disease resistance, this method are to spray alginic acid oligosaccharides in the rice seedling phase to improve defensive ferment in rice leaf
Vigor and disease resistance.
The purpose of the present invention is achieved through the following technical solutions：
A method of rice being improved to rice blast disease resistance using alginic acid oligosaccharides, and the specific operation method is as follows：
Alginic acid oligosaccharides of the present invention is preferably sodium alginate oligosaccharide；
(1) prepared by alginic acid oligosaccharides：1 kilogram of sodium alginate is taken, is dissolved with 100 liters of water, 3% hydrogen peroxide of addition, 80
DEG C, it reacts 90 minutes, is freeze-dried 48 hours, pulverizes.
(2) alginic acid oligosaccharide solution is prepared：The alginic acid oligosaccharides for taking 1 restraint to get ready, is dissolved in the water of certain volume, is made
Solution.
(3) it sprays：Alginic acid oligosaccharide solution of the present invention grows to four leaves wholeheartedly in rice seedling, when about 15-20cm high
Spraying effect is best, and Application is the volume (ml) of alginic acid oligosaccharide solution：Rice area (m2)=100:1~1000:1.
According to technical solution above, in preferred situation, the concentration of the alginic acid oligosaccharides is 0.01%~0.03%.
Beneficial effects of the present invention：
Exciton of the present invention using green non-poisonous alginic acid oligosaccharides as raising paddy disease-resistant ability, at sprinkling
It manages, the Defense Enzyme Activities in the rice seedling of acquisition including phenylalanine lyase, catalase, peroxidase are bright
It is aobvious to be higher than common rice seedling, while the investigation of leaf seasonal febrile diseases and panicle blast is found by late growth stage of rice, disease index
It is decreased obviously, has certain control efficiency to leaf pest and neck fringe pest.The innovation of the invention consists in that the rice seedling phase sprays seaweed
Sour oligosaccharides, using its exciton bioactivity, inducing paddy rice generates defence response, to improve the defence enzyme activity in rice leaf
Power and disease resistance to rice blast, promote the growth of rice.Meanwhile this method is simple and easy, answers conducive to agricultural extension
With.
Specific embodiment
Following nonlimiting examples can with a person of ordinary skill in the art will more fully understand the present invention, but not with
Any mode limits the present invention.
Test involved in the following embodiments of the present invention is as follows：
1. the extraction of enzyme in rice leaf：After sprinkling sodium alginate oligosaccharide solution 3 days, rice leaf is taken, shreds and is placed on
In the mortar of pre-cooling, add suitable Extraction medium, homogenate is ground on ice bath, centrifuged supernatant is enzyme solution to be measured.
2. PAL activity measures：Using L-phenylalanine as substrate, using colorimetric method for determining trans-cinnamic acid
Content, with enzyme amount needed for absorbance increases by 0.01 at the 290nm per hour for an enzyme-activity unit.
3. catalase activity measures：Using hydrogen peroxide as substrate, using colorimetric method for determining content of hydrogen peroxide, with every
Minute enzyme amount needed for absorbance reduction 0.1 at 240nm is an enzyme-activity unit.
4. Peroxidase Activity Determination：Guaiacol colorimetric method, with 0.01 institute of absorbance change at 470nm per minute
Needing enzyme amount is an enzyme-activity unit.
5. the investigation of leaf seasonal febrile diseases：After rice ear sprouting period, 5 points of every cell samplings, every takes 50 plants, carries out severity Scaling
Investigation.
6. the investigation of panicle blast：After rice ear sprouting period, every cell is 50 plants of great-jump-forward investigation parallel, and total fringe is investigated in classification
Several and sick spike number.
7. disease index and control efficiency calculate：
Embodiment 1
(1) prepared by sodium alginate oligosaccharide：1 kilogram of sodium alginate is taken, is dissolved with 100 liters of water, 10 liter of 30% concentration is added
Hydrogen peroxide, 80 DEG C, react 90 minutes, be freeze-dried 48 hours, pulverize.
(2) sodium alginate oligosaccharide solution is prepared：The sodium alginate oligosaccharide for taking 100 restraints to get ready is dissolved in 400 liters of water,
Solution is made.
(3) it sprays：Sodium alginate oligosaccharide solution grows to four leaves wholeheartedly in Dalian experimental plot rice seedling, about 15-
It is sprayed when 20cm high, Application is the volume (ml) of sodium alginate oligosaccharide solution：Rice area (m2)=100:1.
As shown in Table 1, handled by sodium alginate oligosaccharide spray solution, include in rice seedling phenylalanine lyase,
Defense Enzyme Activities including peroxidase, catalase are apparently higher than control, as shown in Table 2, the method treated water
Rice, the disease index of leaf pest and neck fringe pest is significantly lower than control at later stages, and sodium alginate oligosaccharide is to leaf pest and panicle blast
Control efficiency is obvious.
Embodiment 2
(1) prepared by sodium alginate oligosaccharide：1 kilogram of sodium alginate is taken, is dissolved with 100 liters of water, 10 liter of 30% concentration is added
Hydrogen peroxide, 80 DEG C, react 90 minutes, be freeze-dried 48 hours, pulverize.
(2) sodium alginate oligosaccharide solution is prepared：The sodium alginate oligosaccharide for taking 100 restraints to get ready is dissolved in 1000 liters of water,
Solution is made.
(3) it sprays：Sodium alginate oligosaccharide solution grows to four leaves wholeheartedly in Dalian experimental plot rice seedling, about 15-
It is sprayed when 20cm high, Application is the volume (ml) of sodium alginate oligosaccharide solution：Rice area (m2)=1000:1.
As shown in Table 1, handled by sodium alginate oligosaccharide spray solution, include in rice seedling phenylalanine lyase,
Defense Enzyme Activities including peroxidase, catalase are apparently higher than control, as shown in Table 2, the method treated water
Rice, the disease index of leaf pest and neck fringe pest is significantly lower than control at later stages, and sodium alginate oligosaccharide is to leaf pest and panicle blast
Control efficiency is obvious.
Embodiment 3 (blank sample)
Water spray：In Dalian experimental plot, rice seedling grows to four leaves wholeheartedly, carries out when about 15-20cm high at conventional water spray
Reason.
Influence of 1 sodium alginate oligosaccharide of table to Rice Seedling Leaves defensive ferment
Control efficiency of 2 sodium alginate oligosaccharide of table to rice blast
| Testing index | Embodiment 3 | Embodiment 1 | Embodiment 2 |
| Leaf pest disease index | 4.68 | 2.95 | 3.48 |
| Leaf pest control efficiency | - | 36.97% | 25.64% |
| Neck fringe pest disease index | 12.78 | 8.75 | 9.26 |
| Neck fringe pest control efficiency | - | 31.54% | 27.54% |
For any person skilled in the art, without departing from the scope of the technical proposal of the invention, all
Many possible changes and modifications are made to technical solution of the present invention using the technology contents of the disclosure above, or are revised as equivalent
The equivalent embodiment of variation.Therefore, anything that does not depart from the technical scheme of the invention, according to the technical essence of the invention to
Any simple modifications, equivalents, and modifications that upper embodiment is done should all still fall within the range of technical solution of the present invention protection
It is interior.
Claims (5)
1. a kind of improve rice to the method for rice blast disease resistance using alginic acid oligosaccharides, which is characterized in that the rice
Seedling Stage sprays alginic acid oligosaccharide solution.
2. it is according to claim 1 it is a kind of improve rice to the method for rice blast disease resistance using alginic acid oligosaccharides,
It is characterized in that, sprays the volume (ml) of alginic acid oligosaccharide solution：Rice area (m2)=100:1~1000:1.
3. it is according to claim 1 it is a kind of improve rice to the method for rice blast disease resistance using alginic acid oligosaccharides,
It is characterized in that, the alginic acid oligosaccharide solution concentration is 0.01~0.03%.
4. it is according to claim 1 it is a kind of improve rice to the method for rice blast disease resistance using alginic acid oligosaccharides,
It is characterized in that, alginic acid oligosaccharide solution sprinkling when rice seedling grows to 15-20cm high.
5. it is according to claim 1 it is a kind of improve rice to the method for rice blast disease resistance using alginic acid oligosaccharides,
It is characterized in that, the alginic acid oligosaccharide solution is sodium alginate oligosaccharide solution.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109400384A (en) * | 2018-12-19 | 2019-03-01 | 江西省农业科学院植物保护研究所 | Biological organic fertilizer for preventing and treating rice blast and application method thereof |
| CN111990403A (en) * | 2020-09-08 | 2020-11-27 | 北京雷力海洋生物新产业股份有限公司 | Brown algae double-oligoglycoprotein composition, preparation method and application thereof |
| CN112493071A (en) * | 2020-12-01 | 2021-03-16 | 湖南农业大学 | Seedling raising method for rapidly improving cold resistance of mechanical rice transplanting seedlings |
| CN113466132A (en) * | 2021-07-01 | 2021-10-01 | 哈尔滨金融学院 | Hyperspectrum-based rice blast cycle detection equipment |
| CN115428684A (en) * | 2022-10-12 | 2022-12-06 | 云南农业大学 | Method for compensating reduction of blast resistance of OsLOX3 knockout rice strain and verification method and application |
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| CN87107747A (en) * | 1986-10-17 | 1988-06-15 | 明治制菓株式会社 | Plant cultivation method |
| CN105130593A (en) * | 2015-08-06 | 2015-12-09 | 河南省农业科学院植物营养与资源环境研究所 | Sodium alginate oligosaccharide complex and applications of sodium alginate oligosaccharide complex in release of copper toxicity on crops |
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Patent Citations (2)
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| CN87107747A (en) * | 1986-10-17 | 1988-06-15 | 明治制菓株式会社 | Plant cultivation method |
| CN105130593A (en) * | 2015-08-06 | 2015-12-09 | 河南省农业科学院植物营养与资源环境研究所 | Sodium alginate oligosaccharide complex and applications of sodium alginate oligosaccharide complex in release of copper toxicity on crops |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109400384A (en) * | 2018-12-19 | 2019-03-01 | 江西省农业科学院植物保护研究所 | Biological organic fertilizer for preventing and treating rice blast and application method thereof |
| CN109400384B (en) * | 2018-12-19 | 2022-02-25 | 江西省农业科学院植物保护研究所 | Biological organic fertilizer for preventing and treating rice blast and application method thereof |
| CN111990403A (en) * | 2020-09-08 | 2020-11-27 | 北京雷力海洋生物新产业股份有限公司 | Brown algae double-oligoglycoprotein composition, preparation method and application thereof |
| CN111990403B (en) * | 2020-09-08 | 2022-03-25 | 北京雷力海洋生物新产业股份有限公司 | Brown algae double-oligoglycoprotein composition, preparation method and application thereof |
| CN112493071A (en) * | 2020-12-01 | 2021-03-16 | 湖南农业大学 | Seedling raising method for rapidly improving cold resistance of mechanical rice transplanting seedlings |
| CN112493071B (en) * | 2020-12-01 | 2023-10-03 | 湖南农业大学 | Seedling raising method for rapidly improving cold resistance of mechanical transplanted seedlings |
| CN113466132A (en) * | 2021-07-01 | 2021-10-01 | 哈尔滨金融学院 | Hyperspectrum-based rice blast cycle detection equipment |
| CN113466132B (en) * | 2021-07-01 | 2022-04-19 | 哈尔滨金融学院 | Hyperspectrum-based rice blast cycle detection equipment |
| CN115428684A (en) * | 2022-10-12 | 2022-12-06 | 云南农业大学 | Method for compensating reduction of blast resistance of OsLOX3 knockout rice strain and verification method and application |
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Application publication date: 20181116 |