CN113061064A

CN113061064A - Inorganic fertilizer containing microorganisms and preparation method thereof

Info

Publication number: CN113061064A
Application number: CN202110532284.9A
Authority: CN
Inventors: 董芸; 马航; 万邦隆; 李洪姝; 李恒
Original assignee: Yunnan Yuntianhua Co Ltd
Current assignee: Yunnan Yuntianhua Co Ltd
Priority date: 2021-05-17
Filing date: 2021-05-17
Publication date: 2021-07-02

Abstract

The invention discloses an inorganic fertilizer containing microorganisms and a preparation method thereof, and relates to the technical field of novel fertilizers. Mixing various microbial agents to prepare a compound microbial agent, stabilizing and diluting the compound microbial agent, spraying the diluted compound microbial agent on the surface of inorganic fertilizer particles, continuously rolling the fertilizer particles, naturally cooling to room temperature, and quickly bagging for storage. The preparation method has the advantages of simple process, easy operation, mild condition, low energy consumption and low cost; the limit that more than 20 percent of organic matters and no more than 25 percent of inorganic nutrients are added in the prior microbial fertilizer is broken through. The inorganic fertilizer product containing microorganisms does not contain organic matters, the inorganic nutrients in the compound fertilizer are up to more than 45 percent, and the inorganic nutrients in the diammonium phosphate are up to more than 53 percent; and the effective period of the microbial activity is more than 12 months.

Description

Inorganic fertilizer containing microorganisms and preparation method thereof

Technical Field

The invention relates to the technical field of novel fertilizers, in particular to an inorganic fertilizer containing microorganisms and a preparation method thereof.

Background

Inorganic fertilizer, mineral fertilizer, also called chemical fertilizer, has the characteristics of simple components, high effective components and easy absorption by crops, and is mainly prepared by a chemical synthesis method. Wherein the compound fertilizer and the phosphate fertilizer are widely applied.

The compound fertilizer is a chemical fertilizer containing two or more nutrient elements, has the advantages of high nutrient content, few side ingredients, good physical properties and the like, and plays an important role in balancing fertilization, improving the utilization rate of the fertilizer and promoting the high and stable yield of crops. With the mass popularization of the compound fertilizer, the compound fertilizer applied every year at present reaches the ten million ton grade, and the compound fertilizer containing high-concentration inorganic nutrients (N + P) is applied in large quantity₂O₅+K₂The total O nutrient is more than 45 percent), and brings a lot of problems to the soil while improving the crop yield, such as soil hardening, nutrient imbalance, and excessive harmful heavy metal and harmful germ, and finally leads to the crop quality reduction. Part of the nutrient elements can also flow into the water area along with the rainwater, so that the eutrophication of the water body is caused.

The diammonium phosphate as a representative of phosphate fertilizers is a high-efficiency fertilizer widely suitable for vegetables, fruits, rice and wheat, is a basic phosphate fertilizer which is used in large quantities for a long time in China, and is applied in an amount of over 1000 million tons per year. Like compound fertilizers, the fertilizer can not only ensure high and stable yield of crops, but also bring a lot of problems to soil.

In order to increase the brightness and the roundness of finished product particles in the production process, the qualified compound fertilizer particles after granulation and screening are coated and filmed on the outer surfaces of the particles in a coating cylinder, and the diammonium phosphate particles treat the outer surfaces of the particles in the coating cylinder by using coating oil or wax.

In recent years, with the emphasis on ecological protection and green agriculture, the problem of farmland pollution caused by fertilizer use is receiving more and more attention, people also pay attention to the improvement effect of microorganisms on soil, microbial agents and organic matters are applied in a combined manner, and the microbial agents and the organic matters can participate in the fixation of nitrogen in the soil, the conversion of substances and energy and decompose and release nutrients in the organic matters through the life activities of the microorganisms in the soil, so that the fertility of the soil is improved, the effect of improving the soil is achieved, and certain effects are achieved. However, the organic fertilizer has low inorganic components, is difficult to meet the requirements of crops on nitrogen, phosphorus and potassium, and is limited in quality by the viable count and action strength of beneficial microorganisms in the fertilizer, so that high requirements are provided for raw material treatment, production flow and process of the organic fertilizer, the use cost is improved invisibly, and the organic fertilizer is not economical.

If the microbial agent can be combined with inorganic fertilizer and the stable microbial activity of the inorganic fertilizer is maintained, the requirement of crops on inorganic nutrient components can be met, and the improvement of the soil by the microbes can be realized. In the process of combining the microbial agent with an inorganic fertilizer, the microbial activity and the stability are key factors influencing the application effect, the microbial activity of the existing products is highest within half a year, and then obvious attenuation occurs, but the half-year time cannot meet the requirement of the fertilizer shelf life. Therefore, the development of inorganic fertilizer containing microorganisms with high microbial activity and stability and long retention time and a preparation method thereof are needed.

Disclosure of Invention

The invention aims to provide an inorganic fertilizer containing microorganisms and a preparation method thereof, and solves the problems of complex process, high cost, unstable effective viable count and poor application effect of the existing inorganic fertilizer added with a microbial agent.

In order to solve the technical problems, the invention adopts the following technical scheme: an inorganic fertilizer containing microorganisms and a preparation method thereof are characterized in that: the method comprises the following steps of adding a compound microbial agent into a high-concentration inorganic fertilizer:

(1) preparing a compound microbial agent;

(2) stabilizing and diluting the compound microbial agent;

(3) spraying the diluted compound microbial agent on the surface of inorganic fertilizer particles;

(4) continuously rolling the fertilizer particles, naturally cooling to room temperature, and quickly bagging for storage.

The further technical scheme is that the compound microbial agent is prepared by mixing six microbial agents according to the mass ratio of 1:1:1:1:1 at normal temperature, and the effective viable count of each microbial agent is more than 50 hundred million/g.

The further technical scheme is that a stabilizing agent is added in the mixing process of the composite microbial agent, the stabilizing agent is powdered kaolin, diatomite, light calcium carbonate and powdered calcium oxide which are sieved by a 100-mesh sieve, and the adding amount of the stabilizing agent is 1-20 per mill of the mass of the composite microbial agent.

The further technical scheme is that the compound microbial agent is diluted by 10-12 times by using sterile water at normal temperature.

The further technical proposal is that the inorganic fertilizer is compound fertilizer or diammonium phosphate.

The further technical scheme is that the compound microbial agent is prepared by mixing bacillus subtilis, bacillus amyloliquefaciens, bacillus pumilus, bacillus licheniformis, bacillus mucilaginosus and paenibacillus polymyxa.

The further technical scheme is that the diluted compound microbial agent is sprayed from an inlet of a coating barrel for producing the compound fertilizer, the spraying amount is 1-20 per mill of the mass of the compound fertilizer, and the spraying duration is 1-2 minutes.

The further technical scheme is that the diluted compound microbial agent is sprayed from an inlet of a coating drum for producing diammonium phosphate, the spraying amount is 1-20 per mill of the mass of the diammonium phosphate, and the spraying duration is 2-3 minutes.

The working mechanism is as follows:

bacillus subtilis (Bacillus subtilis) is a species of Bacillus. The bacillus subtilis has stronger protease, amylase and lipase activities, can activate in-vivo zymogen into active enzyme, and can also secrete a series of other enzymes, and if collagenase, glucanase, cellulase and the like can help to decompose starch, polysaccharide and other substances in plants. Bacillus subtilis can decompose tryptophan to form indole by using protein, various sugars and starch. Active substances such as subtilin, polymyxin, nystatin, gramicidin and the like can be generated in the growth process of the bacillus subtilis, and the active substances have obvious inhibiting effect on pathogenic bacteria or conditional pathogenic bacteria of endogenous infection.

Paenibacillus polymyxa (Priest & Collins) is a spore-forming, nitrogen-fixing gram-positive bacterium. The bacillus polymyxa has the characteristics of strong nutrition competition capability, high antibacterial substance production, high growth speed, strong adaptability and the like, can quickly grow into dominant flora, occupies ecological advantages, forms a protective film on the surface of a plant, prevents the infection of pathogenic bacteria, and ensures that the pathogenic bacteria have no living space. The paenibacillus polymyxa can be fixedly planted at the root tip of a plant and form a biological membrane, and the biological membrane is accumulated in the space outside the root vascular column, so that the generated biological membrane can greatly accelerate the process that the plant absorbs nutrient substances. The polymyxa corynebacterium gemmifolium can provide nitrogen sources and phosphate fertilizer nutrients which are difficult to be absorbed by plants for the plants through nitrogen fixation and phosphorus dissolution.

Bacillus mucilaginosus (Bacillus mucilaginosus Krassilnikov) can decompose potassium and silicon in minerals such as feldspar, mica and the like, has the functions of dissolving phosphorus, releasing potassium and fixing nitrogen, and can generate substances which are beneficial to plant absorption and utilization such as organic acid, amino acid, polysaccharide, hormone and the like in the growth and propagation process. After being propagated in soil, the plant growth stimulin and various enzymes can be secreted so as to enhance the resistance of crops to some diseases and inhibit the growth of other pathogenic bacteria.

Bacillus pumilus (Bacillus pumilus) is a bacterium of the genus Bacillus with a fine rod-like shape. The Bacillus pumilus can hydrolyze starch and degrade mannan, xylan, cellulose and the like. The probability of producing active substances by the bacillus pumilus is high, and various enzymes such as heat-resistant xylanase, heat-resistant serine alkaline protease, glutamic acid endopeptidase, bilirubin oxidase, collagenase, alkaline cellulase, lipase, enzyme for degrading chlorpyrifos and the like can be continuously produced.

Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) belongs to gram-positive Bacillus, has obvious degradation effect on butachlor, can generate active substances such as low molecular weight antibiotics, antibacterial proteins or polypeptides and the like, inhibits plant pathogenic bacteria, and can be used as rhizosphere bacteria to promote plant growth. Lipopeptide antibiotics with small molecular weight, good thermal stability, D-amino acid content, protease hydrolysis resistance and organic solvent action are synthesized through a non-ribosome approach, and play a main role in inhibiting plant pathogenic bacteria, fungi, viruses and nematodes in biological control application. In addition to secondary metabolites such as antibiotics, a series of extracellular hydrolases which play an important role in the inhibition of pathogenic bacteria are produced, and resistance of host cells is induced. The bacillus amyloliquefaciens can generate a plurality of physiological active substances and amino acid substances such as gibberellin, indoleacetic acid, cytokinin and the like, promote the growth of plant roots and plants, and enhance the disease resistance of the plants, thereby indirectly reducing the occurrence of diseases. The bacillus amyloliquefaciens can degrade ethyl cellulose to generate reducing sugar, can also degrade residual pesticides in soil, and reduces the content of nitrite.

Bacillus licheniformis (Bacillus licheniformis) is a gram-positive thermophilic bacterium commonly found in soil. The bacteria can regulate dysbacteriosis to improve, can produce anti-active substance, has unique biological oxygen-deprivation action mechanism, and can inhibit growth and reproduction of pathogenic bacteria. The bacillus licheniformis has stronger activities of protease, lipase and amylase and can be used for degrading feathers.

Compared with the prior art, the invention has the beneficial effects that:

(1) the preparation method of the inorganic fertilizer containing the microorganisms has the advantages of simple process, easiness in operation, mild condition, low energy consumption and low cost.

(2) The invention breaks through the limitation that more than 20 percent of organic matters and no more than 25 percent of inorganic nutrients are added in the prior microbial fertilizer. The inorganic fertilizer product containing microorganisms does not contain organic matters, the inorganic nutrients in the compound fertilizer are up to more than 45 percent, and the inorganic nutrients in the diammonium phosphate are up to more than 53 percent.

(3) The inorganic fertilizer product containing the microorganisms has the effective period of the microbial activity of more than 12 months.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.

Example 1

A preparation method of a compound fertilizer containing microorganisms comprises the step of mixing bacillus subtilis, bacillus amyloliquefaciens, bacillus pumilus, bacillus licheniformis, bacillus mucilaginosus and paenibacillus polymyxa with effective viable count of more than 50 hundred million/gram at normal temperature according to the mass ratio of 1:1:1:1:1: 1. In the mixing process, 5 per mill of kaolin powder which is 100 meshes is added as a microbial inoculum stabilizer. After mixing, sterile water was slowly added to the mixture in an amount of 10 times the amount of the mixture to dilute the mixture. And diluting to obtain the compound microbial agent. And then accurately spraying the mixture on the surface of the granular compound fertilizer by a calibrated metering pump, wherein the spraying amount is 3 per mill of the mass of the compound fertilizer. The spraying process lasts for about 2 minutes, then the packaging is carried out quickly after the spraying process is cooled to the normal temperature, and double-layer packaging bags are used for packaging.

Example 2

A preparation method of a compound fertilizer containing microorganisms comprises the step of mixing bacillus subtilis, bacillus amyloliquefaciens, bacillus pumilus, bacillus licheniformis, bacillus mucilaginosus and paenibacillus polymyxa with effective viable count of more than 50 hundred million/gram at normal temperature according to the mass ratio of 1:1:1:1:1: 1. In the mixing process, 10 per mill of diatomite powder which is 100 meshes is added as a microbial inoculum stabilizer. After mixing, sterile water was slowly added to the mixture in an amount of 10 times the amount of the mixture to dilute the mixture. And diluting to obtain the compound microbial agent. And then accurately spraying the mixture on the surface of the granular compound fertilizer by a calibrated metering pump, wherein the spraying amount is 3 per mill of the mass of the compound fertilizer. The spraying process lasts for about 2 minutes, then the packaging is carried out quickly after the spraying process is cooled to the normal temperature, and double-layer packaging bags are used for packaging.

Example 3

A preparation method of a compound fertilizer containing microorganisms comprises the step of mixing bacillus subtilis, bacillus amyloliquefaciens, bacillus pumilus, bacillus licheniformis, bacillus mucilaginosus and paenibacillus polymyxa with effective viable count of more than 50 hundred million/gram at normal temperature according to the mass ratio of 1:1:1:1:1: 1. In the mixing process, 5 per mill of light calcium carbonate powder which is 100 meshes over is added as a microbial inoculum stabilizer. After mixing, sterile water was slowly added to the mixture in an amount of 10 times the amount of the mixture to dilute the mixture. And diluting to obtain the compound microbial agent. And then accurately spraying the mixture on the surface of the granular compound fertilizer by a calibrated metering pump, wherein the spraying amount is 3 per mill of the mass of the compound fertilizer. The spraying process lasts for about 2 minutes, then the packaging is carried out quickly after the spraying process is cooled to the normal temperature, and double-layer packaging bags are used for packaging.

Example 4

A preparation method of a compound fertilizer containing microorganisms comprises the step of mixing bacillus subtilis, bacillus amyloliquefaciens, bacillus pumilus, bacillus licheniformis, bacillus mucilaginosus and paenibacillus polymyxa with effective viable count of more than 50 hundred million/gram at normal temperature according to the mass ratio of 1:1:1:1:1: 1. In the mixing process, 5 per mill of calcium oxide powder which passes through 100 meshes is added as a microbial inoculum stabilizer. After mixing, sterile water was slowly added to the mixture in an amount of 10 times the amount of the mixture to dilute the mixture. And diluting to obtain the compound microbial agent. And then accurately spraying the mixture on the surface of the granular compound fertilizer by a calibrated metering pump, wherein the spraying amount is 5 per mill of the mass of the compound fertilizer. The spraying process lasts for about 1 minute, and then the packaging is carried out rapidly after cooling to the normal temperature, and double-layer packaging bags are used for packaging.

Example 5

A preparation method of a compound fertilizer containing microorganisms comprises the step of mixing bacillus subtilis, bacillus amyloliquefaciens, bacillus pumilus, bacillus licheniformis, bacillus mucilaginosus and paenibacillus polymyxa with effective viable count of more than 50 hundred million/gram at normal temperature according to the mass ratio of 1:1:1:1:1: 1. In the mixing process, 5 per mill of diatomite powder which is 100 meshes is added as a microbial inoculum stabilizer. After mixing, sterile water was slowly added to the mixture in an amount of 11 times the amount of the mixture to dilute the mixture. And diluting to obtain the compound microbial agent. And then accurately spraying the mixture on the surface of the granular compound fertilizer by a calibrated metering pump, wherein the spraying amount is 5 per mill of the mass of the compound fertilizer. The spraying process lasts for about 2-3 minutes, and then the packaging is carried out rapidly after the spraying process is cooled to the normal temperature, and a double-layer packaging bag is used for packaging.

Example 6

A preparation method of a compound fertilizer containing microorganisms comprises the step of mixing bacillus subtilis, bacillus amyloliquefaciens, bacillus pumilus, bacillus licheniformis, bacillus mucilaginosus and paenibacillus polymyxa with effective viable count of more than 50 hundred million/gram at normal temperature according to the mass ratio of 1:1:1:1:1: 1. Sterile water is prepared according to the amount diluted by 11 times, 15 per mill of kaolin which is powder and passes through 100 meshes is added into the sterile water to serve as a microbial inoculum stabilizer, and the mixture is uniformly mixed. Then slowly adding prepared sterile water into the compound bacteria mixture for dilution. And diluting to obtain the compound microbial agent. And then accurately spraying the mixture on the surface of the granular compound fertilizer by a calibrated metering pump, wherein the spraying amount is 5 per mill of the mass of the compound fertilizer. The spraying process lasts for about 2-3 minutes, and then the packaging is carried out rapidly after the spraying process is cooled to the normal temperature, and a double-layer packaging bag is used for packaging.

Example 7

A preparation method of diammonium phosphate containing microorganisms comprises the step of mixing bacillus subtilis, bacillus amyloliquefaciens, bacillus pumilus, bacillus licheniformis, bacillus mucilaginosus and paenibacillus polymyxa with effective viable count of more than 50 hundred million/gram at normal temperature according to the mass ratio of 1:1:1:1:1: 1. 1 per mill of kaolin powder which is 100 meshes is added in the mixing process as a microbial agent stabilizer. After mixing, sterile water was slowly added to the mixture in an amount of 10 times the amount of the mixture to dilute the mixture. And diluting to obtain the compound microbial agent. And then accurately spraying the diammonium phosphate particles onto the surfaces of the granular diammonium phosphate particles by using a calibrated metering pump, wherein the spraying amount is 3 per mill of the mass of the diammonium phosphate. The spraying process lasts for about 2-3 minutes, and then the packaging is carried out rapidly after the spraying process is cooled to the normal temperature, and a double-layer packaging bag is used for packaging.

Example 8

A preparation method of diammonium phosphate containing microorganisms comprises the step of mixing bacillus subtilis, bacillus amyloliquefaciens, bacillus pumilus, bacillus licheniformis, bacillus mucilaginosus and paenibacillus polymyxa with effective viable count of more than 50 hundred million/gram at normal temperature according to the mass ratio of 1:1:1:1:1: 1. 1 per mill of diatomite powder which is 100 meshes is added in the mixing process to be used as a microbial inoculum stabilizer. After mixing, sterile water was slowly added to the mixture in an amount of 10 times the amount of the mixture to dilute the mixture. And diluting to obtain the compound microbial agent. And then accurately spraying the diammonium phosphate particles onto the surfaces of the granular diammonium phosphate particles by using a calibrated metering pump, wherein the spraying amount is 3 per mill of the mass of the diammonium phosphate. The spraying process lasts for about 2-3 minutes, and then the packaging is carried out rapidly after the spraying process is cooled to the normal temperature, and a double-layer packaging bag is used for packaging.

Example 9

A preparation method of diammonium phosphate containing microorganisms comprises the step of mixing bacillus subtilis, bacillus amyloliquefaciens, bacillus pumilus, bacillus licheniformis, bacillus mucilaginosus and paenibacillus polymyxa with effective viable count of more than 50 hundred million/gram at normal temperature according to the mass ratio of 1:1:1:1:1: 1. In the mixing process, 1 per mill of light calcium carbonate powder which is 100 meshes over is added as a microbial inoculum stabilizer. After mixing, sterile water was slowly added to the mixture in an amount of 10 times the amount of the mixture to dilute the mixture. And diluting to obtain the compound microbial agent. And then accurately spraying the diammonium phosphate particles onto the surfaces of the granular diammonium phosphate particles by using a calibrated metering pump, wherein the spraying amount is 3 per mill of the mass of the diammonium phosphate. The spraying process lasts for about 2-3 minutes, and then the packaging is carried out rapidly after the spraying process is cooled to the normal temperature, and a double-layer packaging bag is used for packaging.

Example 10

A preparation method of diammonium phosphate containing microorganisms comprises the step of mixing bacillus subtilis, bacillus amyloliquefaciens, bacillus pumilus, bacillus licheniformis, bacillus mucilaginosus and paenibacillus polymyxa with effective viable count of more than 50 hundred million/gram at normal temperature according to the mass ratio of 1:1:1:1:1: 1. During the mixing process, 1 per mill of calcium oxide powder which passes through 100 meshes is added as a microbial inoculum stabilizer. After mixing, sterile water was slowly added to the mixture in an amount of 10 times the amount of the mixture to dilute the mixture. And diluting to obtain the compound microbial agent. And then accurately spraying the diammonium phosphate particles onto the surfaces of the granular diammonium phosphate particles by using a calibrated metering pump, wherein the spraying amount is 3 per mill of the mass of the diammonium phosphate. The spraying process lasts for about 2-3 minutes, and then the packaging is carried out rapidly after the spraying process is cooled to the normal temperature, and a double-layer packaging bag is used for packaging.

Example 11

A preparation method of diammonium phosphate containing microorganisms comprises the step of mixing bacillus subtilis, bacillus amyloliquefaciens, bacillus pumilus, bacillus licheniformis, bacillus mucilaginosus and paenibacillus polymyxa with effective viable count of more than 50 hundred million/gram at normal temperature according to the mass ratio of 1:1:1:1:1: 1. 3 per mill of diatomite powder which is 100 meshes is added in the mixing process to be used as a microbial inoculum stabilizer. After mixing, sterile water was slowly added to the mixture in an amount of 10 times the amount of the mixture to dilute the mixture. And diluting to obtain the compound microbial agent. And then accurately spraying the diammonium phosphate particles onto the surfaces of the granular diammonium phosphate particles by using a calibrated metering pump, wherein the spraying amount is 3 per mill of the mass of the diammonium phosphate. The spraying process lasts for about 2-3 minutes, and then the packaging is carried out rapidly after the spraying process is cooled to the normal temperature, and a double-layer packaging bag is used for packaging.

Example 12

A preparation method of diammonium phosphate containing microorganisms comprises the step of mixing bacillus subtilis, bacillus amyloliquefaciens, bacillus pumilus, bacillus licheniformis, bacillus mucilaginosus and paenibacillus polymyxa with effective viable count of more than 50 hundred million/gram at normal temperature according to the mass ratio of 1:1:1:1:1: 1. Sterile water is prepared according to the amount of 10 times dilution, 1 thousandth of kaolin which is powder and passes through 100 meshes is added into the sterile water to serve as a microbial inoculum stabilizer, and the mixture is uniformly mixed. Then slowly adding prepared sterile water into the compound bacteria mixture for dilution. And diluting to obtain the compound microbial agent. And then accurately spraying the diammonium phosphate particles onto the surfaces of the granular diammonium phosphate particles by using a calibrated metering pump, wherein the spraying amount is 3 per mill of the mass of the diammonium phosphate. The spraying process lasts for about 2-3 minutes, and then the packaging is carried out rapidly after the spraying process is cooled to the normal temperature, and a double-layer packaging bag is used for packaging.

Example 13

The microbial activity of the two products is regularly detected, the detection results are shown in table 1, and the table 1 shows that the microbial activity of the product is stable and does not obviously decline within at least 12 months.

TABLE 1 detection results of microbial activity of inorganic fertilizer containing microbes in shelf life

Note: the shelf life of the compound fertilizer detection is 14 months;

shelf life at compound fertilizer detection was 20 months.

Simultaneously, the two products are subjected to field tests, which specifically comprise the following steps:

experimental example 1

In order to verify the application effect of the microbial diammonium phosphate-containing fertilizer on northern winter wheat, a field test is designed to research the influence of the microbial diammonium phosphate-containing fertilizer on the agronomic characters and the yield of the winter wheat.

The test position is in Nanjing Zhendong Bao village of Yuanshi county, Hebei province. The soil fertility of the land is medium and uniform, well water irrigation is performed, the production condition is good, and the land is a main production area for producing wheat in Hebei. Soil type: calcareous brown soil, soil texture: the middle soil. The soil nutrient content is as follows: 26.2g/kg of organic matter, 1.08g/kg of total nitrogen, 29mg/kg of available phosphorus, 73mg/kg of quick-acting potassium and 8.1 of PH value.

The fertilizer is common diammonium phosphate (18-46-0), microbial diammonium phosphate (15-39-0), common urea (N-46) and potassium chloride.

The tested crop is wheat, and the variety is Jimai 518.

The experiment designed 2 treatments, 4 replicates, 30m per cell²And arranging protection rows around the test field according to the designed dosage of equal nutrients for each treated fertilizer. The fertilization mode is basal application/topdressing, the application mode adopts a local conventional mode, and the seeding density is according to the local conventional density. The experimental treatment design was as follows:

treatment 1: conventional treatment (bottom application of common diammonium phosphate (18-46-0)25 kg/mu + common urea 16 kg/mu, common potassium chloride 4 kg/mu, topdressing common urea 15 kg/mu)

And (3) treatment 2: bottom application of No. 1 microbial diammonium phosphate containing 29.5 kg/mu and common urea containing 16 kg/mu; common potassium chloride is 4 kg/mu; topdressing of 15 kg/mu of common urea

In the field experiment, soil preparation and fertilization are carried out 10 and 11 days in 2019, diammonium phosphate base fertilizer is applied at one time in each treatment, seeding is carried out 10 and 12 days in 10 months, the seeding quantity per mu is 14 kg, the planting is carried out at equal row spacing, and the row spacing is 15 cm. Watering and fertilizing for 11-19 days, wherein urea applied at the bottom is applied at one time, and herbicide is sprayed for 11-25 days; watering and fertilizing at 3-25 days in 2020, topdressing the variety and the dosage according to the experimental design in each treatment, comprehensively preventing and treating the plant diseases and insect pests for 2 times at 1-8 days in 5-month period, and harvesting at 13-6 months in 2020.

The number of stems per mu, single tiller and secondary rooting of winter wheat are investigated in the wheat growing period, and the investigation results are shown in table 2.

TABLE 2 winter wheat growing period fertility survey table

Treatment of	Single tiller	Secondary root (strip)	Mu Stem number (ten thousand)
				1	2.92	9.65	87.6
2	3.1	10.95	94.15

The ear number per mu, the grain number per ear and the thousand grain weight of the wheat are investigated in the mature period of the wheat, and the investigation results are shown in table 3.

TABLE 3 questionnaire of wheat maturity fertility traits

Treatment of	Mu ear number (average value)	Grain number per ear (average value)	Thousand Kernel weight (mean value)
				1	47.69	22.95	41.08
2	48.48	24.12	41.63

The test cell yield measurement method adopts that each cell selects 3 representative point locations, each point is checked for the single row spike number (converted to the mu spike number) of 1 meter, meanwhile, each point selects 10 spikes for investigation of the spike number and the thousand kernel weight, and the theoretical per mu yield is calculated by 3 investigation point values on average. The results of the yield calculations are shown in Table 4.

Table 4 statistical units of yield: kg/mu

From table 4 it follows that: compared with treatment of 1 mu, the treatment 2 increases the yield by 30.63kg, and the yield is increased by 8.02%.

And (4) test conclusion:

the growth and development of the wheat can be effectively promoted by using the microbial diammonium phosphate-containing winter wheat, and the yield of the wheat is increased. Meanwhile, the spike number and the grain number of each mu are improved, and the thousand grain weight is increased.

Experimental example 2

In order to verify the field application effect of the compound fertilizer containing the microorganisms on vegetables, the experiment is designed to research the influence of the compound fertilizer on the agronomic traits and the yield of the vegetables.

The test site is located in the Stone shoal town of the city-increasing district of Guangzhou city, Guangdong province.

And (3) test crops: lettuce in Italian.

And (3) testing fertilizers: conventional compound fertilizer (15-15-15) and microbial compound fertilizer (15-15-15).

And (3) experimental design: the experiment was set up with 2 treatments in total, for comparison with the field.

Processing one: conventional control group (bottom application compound fertilizer (15-15-15)25 kg/mu, topdressing compound fertilizer (15-15-15)25 kg/mu)

And (5) processing: containing microorganism compound fertilizer (bottom application containing microorganism compound fertilizer (15-15-15)25 kg/mu, topdressing containing microorganism compound fertilizer (15-15-15)25 kg/mu)

The test is carried out by burying base fertilizer in field planting in 12 months in 2018, topdressing in 1 month in 2019 and harvesting in 3 days in 2 months.

After the test, plant samples were obtained from the test field, and the root length, plant height, stem thickness and dry matter content were measured, and the statistical test yields are shown in table 5.

TABLE 5

And (4) test conclusion:

as can be seen from Table 5, the compound fertilizer containing the microorganisms has a certain improvement effect on the agronomic traits in lettuce planting, and the yield can be increased to 6.41% under the condition of equal nutrient application amount.

The above examples of the present invention are only for illustrating the present invention and are not intended to limit the embodiments of the present invention. Variations or modifications in other variations will be apparent to persons skilled in the art based on the foregoing description. It is not necessary to exhaustively enumerate all embodiments herein. Any modification, equivalent replacement, and improvement made within the spirit and principle of the present invention should be included in the protection scope of the claims of the present invention.

Claims

1. A preparation method of inorganic fertilizer containing microorganisms is characterized by comprising the following steps: the method comprises the following steps of adding a compound microbial agent into a high-concentration inorganic fertilizer:

(1) preparing a compound microbial agent;

(2) stabilizing and diluting the compound microbial agent;

2. The method for preparing inorganic fertilizer containing microorganism as claimed in claim 1, wherein: the compound microbial agent is prepared by mixing six microbial agents according to the mass ratio of 1:1:1:1:1:1 at normal temperature, and the effective viable count of each microbial agent is more than 50 hundred million/g.

3. The method for preparing inorganic fertilizer containing microorganism as claimed in claim 2, wherein: the composite microbial agent is added with a stabilizer in the mixing process, wherein the stabilizer is powdered kaolin, diatomite, light calcium carbonate and powdered calcium oxide which are sieved by a 100-mesh sieve, and the adding amount of the stabilizer is 1-20 per mill of the mass of the composite microbial agent.

4. The method for preparing inorganic fertilizer containing microorganism as claimed in claim 1, wherein: the compound microbial agent is diluted by 10-12 times by using sterile water at normal temperature.

5. The method for preparing inorganic fertilizer containing microorganism as claimed in claim 1, wherein: the inorganic fertilizer is a compound fertilizer or diammonium phosphate.

6. The method for preparing inorganic fertilizer containing microorganism as claimed in claim 1, wherein: the compound microbial agent is prepared by mixing bacillus subtilis, bacillus amyloliquefaciens, bacillus pumilus, bacillus licheniformis, bacillus mucilaginosus and paenibacillus polymyxa.

7. The method for preparing inorganic fertilizer containing microorganisms as claimed in claim 5, wherein: the diluted compound microbial agent is sprayed from an inlet of a wrapping drum for producing the compound fertilizer, the spraying amount is 1-20 per mill of the mass of the compound fertilizer, and the spraying duration is 1-2 minutes.

8. The method for preparing inorganic fertilizer containing microorganisms as claimed in claim 5, wherein: the diluted compound microbial agent is sprayed from an inlet of a coating drum for producing diammonium phosphate, the spraying amount is 1-20 per mill of the mass of the diammonium phosphate, and the spraying duration is 2-3 minutes.

9. An inorganic fertilizer containing microorganisms is characterized in that: the inorganic fertilizer containing the microorganism, which is prepared by the preparation method of the inorganic fertilizer containing the microorganism, as claimed in any one of claims 1 to 8.