CN106922249A - A kind of germination treatment method of wax of use dilute sulfuric acid to beet Wild related germplasm - Google Patents
A kind of germination treatment method of wax of use dilute sulfuric acid to beet Wild related germplasm Download PDFInfo
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- CN106922249A CN106922249A CN201710245644.0A CN201710245644A CN106922249A CN 106922249 A CN106922249 A CN 106922249A CN 201710245644 A CN201710245644 A CN 201710245644A CN 106922249 A CN106922249 A CN 106922249A
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- beet
- sulfuric acid
- dilute sulfuric
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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Abstract
A kind of germination treatment method of wax of use dilute sulfuric acid to beet Wild related germplasm, it is related to technical field of plant propagation, the technical problem to be solved in the present invention is that beet seed speed of germination is slow, germination percentage is low, complex operation, the reagent cost for using is high, and has pollution, is not easy to the problem of industrialized production.The method includes:(1) seed is gathered；(2) seed is cleaned；(3) seed is processed with dilute sulfuric acid；(4) clear water rinses the treated seed of dilute sulfuric acid；(5) seed after rinsing is placed in germination box by wet gauze parcel, is placed on culture in constant incubator, and period cleans seed, changes clean wet gauze with clear water daily.Continuous through the above way to cultivate 23 weeks, beet seed germinates substantially, then transplants.The method is simple to operate, low cost, efficiently solves the problems, such as that beet seed speed of germination is slow, promotes the breeding research of beet, improves the yield of beet.
Description
Technical field
The present invention relates to technical field of plant propagation, more particularly to a kind of side for promoting beet Wild related germplasm to germinate
Method.
Background technology
Beet (Beta vulgaris), You Ming Chard dishes, biennial herb plant originates in western European and Southern Coast,
It is the main sugar source beyond tropical sugarcane.Sugar beet originates from Mediterranean, China is introduced in 1906, as me
One of the main sugar industry crop in the state north.
The beet of northeast plantation accounts for the 65% of national total area, and this area is spring sowing beet area, and frost-free period is short, accumulated temperature compared with
Few, sunshine is big compared with strong, day and night temperature, and the per unit area yield and sugar content of beet are high, disease is light.Beet be all from head to foot it is precious, beet it is main
Product is sugar, and sugar is people's indispensable nutriment of life, is also the important source material of food industry and medical industry.Except
Outside production sucrose, beet and its byproduct also have extensive exploitation Utilization prospects.The cauline leaf of beet can be managed except carrotene is rich in
Outside the succulence green feed thought, fertilizer also field is also used as, increases the content of organic matter in soil.Beet sugar manufacture industrial by-product
Product put into when being mainly in root tuber 3.5% or so sugar and 7.5% or so non-sugar ingredient and processing and discharge other are non-
Sugar substance.
Beet seed be in biconvex mirror shape, diameter 2-3mm, bronzing is glossy.Seed pericarp is thick and lignifying, hard, inhales
Water is slow, and germination percentage is low, germinates irregular, is unfavorable for full stand and Qi Miao, has considerable influence to production, and the country is to beet seed
The research of germination technology is relatively on the low side, and the germination quality of beet seed, beet kind are improved currently without a kind of effective method
The relatively low problem of son germination quality is needed badly and is resolved.
At present, in the market coordinates the modes such as nutriment to promote seed to sprout using hormone mostly, raising germination percentage, and this
Kind of the most high cost of mode, not environmentally, and cumbersome is not easy to industrialized production.Sprouting to how to improve beet seed
Rate, reduces environmental pollution, and industrialized production does not have reference, therefore urgent need one kind can effectively solve the problem that above mentioned problem now
Beet seed germination method.
The content of the invention
Slow the present invention solves the technical problem of beet seed speed of germination, germination percentage is low, complex operation, uses
Reagent cost is high, is not easy to the problem of industrialized production, and provides a kind of use dilute sulfuric acid to beet Wild related germplasm
Germination treatment method of wax.
To the germination treatment method of wax of beet Wild related germplasm, the method includes following a kind of use dilute sulfuric acid of the invention
Step：
First, collection beet Wild related germplasm, clear water rinses seed；
2nd, the beet seed after flushing is immersed in dilute sulfuric acid, 30~40s of soaking at room temperature；
3rd, the beet seed after dilute sulfuric acid is soaked is put into clear water, is cleaned 5 times repeatedly；
4th, in germination box inner bottom part three layers of wet gauze of tiling, above-mentioned treated beet seed is placed on wet gauze,
Three layers of wet gauze of lid again above beet seed, then will be integrally placed at continuous culture 2~3 weeks in constant incubator, that is, complete institute
Germination treatment of the use dilute sulfuric acid stated to beet Wild related germplasm.
The present invention includes following beneficial effect：
Relative to existing method (such as dioxygen water process), (germination percentage can for the germination percentage for significantly improving beet seed of the invention
Up to 95%, improve 20% or so compared with dioxygen water process), (beet seed is in 2-3 for the germinating time of shortening beet seed
Germinateed substantially when all), and the method low cost, it is more easy to operate, and it is easy to industrialized production.The present invention is clear only with dilute sulfuric acid
Seed is washed, the germination speed of beet seed just can be effectively improved, shortens germinating time, there is provided one kind is environmentally friendly and is easy to industrialization
The technology of big production, greatlys save production cost, with great application value, promotes to sprout with existing use various reagents
Hair is compared, and the method for the present invention does not pollute to environment, decreases the triviality of operation, is a kind of skill of great popularization
Art.
Specific embodiment
Specific embodiment one：A kind of germination treatment of the use dilute sulfuric acid of present embodiment to beet Wild related germplasm
Method, the method is comprised the following steps：
First, collection beet Wild related germplasm, clear water rinses seed；
2nd, the beet seed after flushing is immersed in dilute sulfuric acid, 30~40s of soaking at room temperature；
3rd, the beet seed after dilute sulfuric acid is soaked is put into clear water, is cleaned 5 times repeatedly；
4th, in germination box inner bottom part three layers of wet gauze of tiling, above-mentioned treated beet seed is placed on wet gauze,
Three layers of wet gauze of lid again above beet seed, then will be integrally placed at continuous culture 2~3 weeks in constant incubator, that is, complete institute
Germination treatment of the use dilute sulfuric acid stated to beet Wild related germplasm.
Specific embodiment two：Present embodiment from unlike specific embodiment one：Described dilute sulfuric acid concentration is
≤4mol/L.Other are identical with specific embodiment one.
Specific embodiment three：Present embodiment from unlike specific embodiment one：Described dilute sulfuric acid concentration is
≤3mol/L.Other are identical with specific embodiment one.
Specific embodiment four：Present embodiment from unlike specific embodiment one：Described dilute sulfuric acid concentration is
≤2mol/L.Other are identical with specific embodiment one.
Specific embodiment five：Present embodiment from unlike specific embodiment one：Described dilute sulfuric acid concentration is
≤1mol/L.Other are identical with specific embodiment one.
Specific embodiment six：Present embodiment from unlike specific embodiment one：Described dilute sulfuric acid concentration is
≤0.5mol/L.Other are identical with specific embodiment one.
Specific embodiment seven：Present embodiment from unlike specific embodiment one：Described clear water temperature is
15~20 DEG C.Other are identical with specific embodiment one.
Specific embodiment eight：Present embodiment from unlike specific embodiment one：Described clear water temperature is
18~20 DEG C.Other are identical with specific embodiment one.
Specific embodiment nine：Present embodiment from unlike specific embodiment one：The temperature of constant incubator is
22~25 DEG C, and in the training period, clean wet gauze need to be changed once daily, and rinse a beet seed with clear water.Its
It is identical with specific embodiment one.
Specific embodiment ten：Present embodiment from unlike specific embodiment one：The humidity of described wet gauze
It is advisable to twist not water outlet.Other are identical with specific embodiment one.
Present invention is not limited only to the content of the respective embodiments described above, the group of one of them or several specific embodiments
Contract sample can also realize the purpose of invention.
Verify of the invention have with effect by following examples：
Example 1
To the germination treatment method of wax of beet Wild related germplasm, it is according to following to a kind of use dilute sulfuric acid of the present embodiment
What step was carried out：
(1) selected 100 full beet Wild related germplasm, after being rinsed well through clear water, concentration are immersed in by seed
In for the dilution heat of sulfuric acid of 4moL/L, seed is pulled out immediately after continuing 30s, and rinsed repeatedly with clear water 5 times；
(2) (wet gauze is twisted not water outlet and is advisable with hand, and gauze and germination box need to be in advance in germination cassette bottom layer to spread three layers of wet gauze
Sterilize 20min at 121 DEG C), then by the above-mentioned evenly spaced row of treated seed on wet gauze, on seed again
Three layers of wet gauze of lid；
The above-mentioned germination box for completing wet gauze and seed is put in constant incubator, incubated at 22 DEG C, culture
Period cleans seed once using clear water daily.The quantity of chitting piece is counted respectively when cultivating to 2 weeks, 3 weeks, and calculates germination
Rate, as a result sees attached list 1.
Example 2
To the germination treatment method of wax of beet Wild related germplasm, it is according to following to a kind of use dilute sulfuric acid of the present embodiment
What step was carried out：
(1) selected 100 full beet Wild related germplasm, after being rinsed well through clear water, concentration are immersed in by seed
In for the dilution heat of sulfuric acid of 3moL/L, seed is pulled out immediately after continuing 40s, and rinsed repeatedly with clear water 5 times；
(2) (wet gauze is twisted not water outlet and is advisable with hand, and gauze and germination box need to be in advance in germination cassette bottom layer to spread three layers of wet gauze
Sterilize 20min at 121 DEG C), then by the above-mentioned evenly spaced row of treated seed on wet gauze, on seed again
Three layers of wet gauze of lid；
(3) the above-mentioned germination box for completing wet gauze and seed is put in constant incubator, incubated at 25 DEG C, training
Seed is cleaned during supporting once using clear water daily.The quantity of chitting piece is counted respectively when cultivating to 2 weeks, 3 weeks, and calculates hair
Bud rate, as a result sees attached list 1.
Sprouting of the different disposal condition of table 1 on beet seed influences
| 2 weeks | 3 weeks | |
| The percentage of seedgermination of example 1 | 88% | 97% |
| The percentage of seedgermination of example 2 | 86% | 98% |
Beet seed after the treatment of example 1 and 2 germinateed substantially at 2-3 weeks, and best in quality.
Embodiment 3
To the germination treatment method of wax of beet Wild related germplasm, it is according to following to a kind of use dilute sulfuric acid of the present embodiment
What step was carried out：
(1) selected 100 full beet Wild related germplasm, after being rinsed well through clear water, concentration are immersed in by seed
In for the dilution heat of sulfuric acid of 2moL/L, seed is pulled out immediately after continuing 40s, and rinsed repeatedly with clear water 5 times；
(2) (wet gauze is twisted not water outlet and is advisable with hand, and gauze and germination box need to be in advance in germination cassette bottom layer to spread three layers of wet gauze
Sterilize 20min at 121 DEG C), then by the above-mentioned evenly spaced row of treated seed on wet gauze, on seed again
Three layers of wet gauze of lid；
The above-mentioned germination box for completing wet gauze and seed is put in constant incubator, incubated at 22 DEG C, culture
Period cleans seed once using clear water daily.
Embodiment 4
To the germination treatment method of wax of beet Wild related germplasm, it is according to following to a kind of use dilute sulfuric acid of the present embodiment
What step was carried out：
(1) selected 100 full beet Wild related germplasm, after being rinsed well through clear water, concentration are immersed in by seed
In for the dilution heat of sulfuric acid of 1moL/L, seed is pulled out immediately after continuing 40s, and rinsed repeatedly with clear water 5 times；
(2) (wet gauze is twisted not water outlet and is advisable with hand, and gauze and germination box need to be in advance in germination cassette bottom layer to spread three layers of wet gauze
Sterilize 20min at 121 DEG C), then by the above-mentioned evenly spaced row of treated seed on wet gauze, on seed again
Three layers of wet gauze of lid；
The above-mentioned germination box for completing wet gauze and seed is put in constant incubator, incubated at 22 DEG C, culture
Period cleans seed once using clear water daily.
Embodiment 5
To the germination treatment method of wax of beet Wild related germplasm, it is according to following to a kind of use dilute sulfuric acid of the present embodiment
What step was carried out：
(1) selected 100 full beet Wild related germplasm, after being rinsed well through clear water, concentration are immersed in by seed
In for the dilution heat of sulfuric acid of 0.5moL/L, seed is pulled out immediately after continuing 40s, and rinsed repeatedly with clear water 5 times；
(2) (wet gauze is twisted not water outlet and is advisable with hand, and gauze and germination box need to be in advance in germination cassette bottom layer to spread three layers of wet gauze
Sterilize 20min at 121 DEG C), then by the above-mentioned evenly spaced row of treated seed on wet gauze, on seed again
Three layers of wet gauze of lid；
The above-mentioned germination box for completing wet gauze and seed is put in constant incubator, incubated at 22 DEG C, culture
Period cleans seed once using clear water daily.
The germination rate of the beet seed after the treatment of embodiment 3 to 5 can reach 90% or so after 3 weeks, in 2~3 weeks bases
This is all sprouted.
By above example, the method for the present invention just can provide raising well only with dilution heat of sulfuric acid
Beet seed germination rate, for industrialized production provides basis.And the dilution heat of sulfuric acid treatment beet seed that the present invention is used
The condition optimizing of method is not limited to the content cited by above example, and any mode for not departing from inventive concept can reach
To the purpose of the present invention, belong to protection scope of the present invention.
General principle of the invention and operating process are the foregoing described, the present invention is not limited to the above embodiments, it is above-mentioned
Simply general principle of the invention described in embodiment and specification.The claimed scope of the invention is by appended claim
Book and its equivalent thereof.
Claims (10)
1. a kind of use dilute sulfuric acid to beet Wild related germplasm germination treatment method of wax, it is characterised in that the method include it is following
Step：
First, collection beet Wild related germplasm, clear water rinses seed；
2nd, the beet seed after flushing is immersed in dilute sulfuric acid, 30~40s of soaking at room temperature；
3rd, the beet seed after dilute sulfuric acid is soaked is put into clear water, is cleaned 5 times repeatedly；
4th, in germination box inner bottom part three layers of wet gauze of tiling, above-mentioned treated beet seed is placed on wet gauze, in beet
Three layers of wet gauze of lid again above seed, then will be integrally placed at continuous culture 2~3 weeks in constant incubator, that is, complete described in
Using the dilute sulfuric acid germination treatment sub to beet Wild related germplasm.
2. a kind of use dilute sulfuric acid according to claim 1 is to the germination treatment method of wax of beet Wild related germplasm, and it is special
Levy is that described dilute sulfuric acid concentration is≤4mol/L.
3. a kind of use dilute sulfuric acid according to claim 2 is to the germination treatment method of wax of beet Wild related germplasm, and it is special
Levy is that described dilute sulfuric acid concentration is≤3mol/L.
4. a kind of use dilute sulfuric acid according to claim 3 is to the germination treatment method of wax of beet Wild related germplasm, and it is special
Levy is that described dilute sulfuric acid concentration is≤2mol/L.
5. a kind of use dilute sulfuric acid according to claim 4 is to the germination treatment method of wax of beet Wild related germplasm, and it is special
Levy is that described dilute sulfuric acid concentration is≤1mol/L.
6. a kind of use dilute sulfuric acid according to claim 5 is to the germination treatment method of wax of beet Wild related germplasm, and it is special
Levy is that described dilute sulfuric acid concentration is≤0.5mol/L.
7. a kind of use dilute sulfuric acid according to claim 1 is to the germination treatment method of wax of beet Wild related germplasm, and it is special
Levy is that described clear water temperature is 15~20 DEG C.
8. a kind of use dilute sulfuric acid according to claim 1 is to the germination treatment method of wax of beet Wild related germplasm, and it is special
Levy is that described clear water temperature is 18~20 DEG C.
9. a kind of use dilute sulfuric acid according to claim 1 is to the germination treatment method of wax of beet Wild related germplasm, and it is special
Levy be constant incubator temperature for 22~25 DEG C, and in the training period, clean wet gauze need to be changed once daily, be used in combination
Clear water rinses a beet seed.
10. a kind of use dilute sulfuric acid according to claim 1 is to the germination treatment method of wax of beet Wild related germplasm, and it is special
The humidity for levying the wet gauze described in being is to twist not water outlet to be advisable.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710245644.0A CN106922249A (en) | 2017-04-14 | 2017-04-14 | A kind of germination treatment method of wax of use dilute sulfuric acid to beet Wild related germplasm |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710245644.0A CN106922249A (en) | 2017-04-14 | 2017-04-14 | A kind of germination treatment method of wax of use dilute sulfuric acid to beet Wild related germplasm |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1042817A (en) * | 1989-12-20 | 1990-06-13 | 内蒙古大学 | A kind of seed processing technology that improves the beet yield and quality |
| CN105191540A (en) * | 2015-09-25 | 2015-12-30 | 榕江平阳生态源开发有限责任公司 | Cyclocarya paliurus seed soaking method |
| CN105993286A (en) * | 2016-07-13 | 2016-10-12 | 安徽菲扬农业科技有限公司 | Method for germinating beet seeds |
-
2017
- 2017-04-14 CN CN201710245644.0A patent/CN106922249A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1042817A (en) * | 1989-12-20 | 1990-06-13 | 内蒙古大学 | A kind of seed processing technology that improves the beet yield and quality |
| CN105191540A (en) * | 2015-09-25 | 2015-12-30 | 榕江平阳生态源开发有限责任公司 | Cyclocarya paliurus seed soaking method |
| CN105993286A (en) * | 2016-07-13 | 2016-10-12 | 安徽菲扬农业科技有限公司 | Method for germinating beet seeds |
Non-Patent Citations (1)
| Title |
|---|
| 方元炜译: "感染锈病的糖用甜菜种子的检疫处理", 《种子世界》 * |
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Application publication date: 20170707 |
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