CN106916837A - 高渗透压甘油蛋白激酶基因RkHog1及其重组表达载体 - Google Patents
高渗透压甘油蛋白激酶基因RkHog1及其重组表达载体 Download PDFInfo
- Publication number
- CN106916837A CN106916837A CN201710207454.XA CN201710207454A CN106916837A CN 106916837 A CN106916837 A CN 106916837A CN 201710207454 A CN201710207454 A CN 201710207454A CN 106916837 A CN106916837 A CN 106916837A
- Authority
- CN
- China
- Prior art keywords
- gene
- rkhog1
- hyperosmosis
- glycerine
- protein kinase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 title claims abstract description 44
- 235000011187 glycerol Nutrition 0.000 title claims abstract description 20
- 108060006633 protein kinase Proteins 0.000 title claims abstract description 15
- 238000003259 recombinant expression Methods 0.000 title claims abstract description 9
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 25
- 125000003729 nucleotide group Chemical group 0.000 claims abstract description 6
- 239000002773 nucleotide Substances 0.000 claims abstract description 5
- 125000003275 alpha amino acid group Chemical group 0.000 claims abstract 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 abstract description 29
- 230000033228 biological regulation Effects 0.000 abstract description 7
- 102000001253 Protein Kinase Human genes 0.000 abstract description 5
- 150000003839 salts Chemical class 0.000 abstract 1
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 27
- 239000013612 plasmid Substances 0.000 description 25
- 238000006243 chemical reaction Methods 0.000 description 22
- 101100395423 Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) hog-1 gene Proteins 0.000 description 19
- 241000894006 Bacteria Species 0.000 description 14
- 241000221523 Rhodotorula toruloides Species 0.000 description 13
- 238000012217 deletion Methods 0.000 description 12
- 230000037430 deletion Effects 0.000 description 12
- ISAKRJDGNUQOIC-UHFFFAOYSA-N Uracil Chemical compound O=C1C=CNC(=O)N1 ISAKRJDGNUQOIC-UHFFFAOYSA-N 0.000 description 10
- 238000012408 PCR amplification Methods 0.000 description 8
- 241000007100 Rhodotorula kratochvilovae Species 0.000 description 8
- 230000001580 bacterial effect Effects 0.000 description 8
- 230000003834 intracellular effect Effects 0.000 description 8
- 230000019491 signal transduction Effects 0.000 description 8
- 241000233866 Fungi Species 0.000 description 7
- 230000035508 accumulation Effects 0.000 description 7
- 238000009825 accumulation Methods 0.000 description 7
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 6
- 238000013459 approach Methods 0.000 description 6
- 230000029087 digestion Effects 0.000 description 6
- 230000014509 gene expression Effects 0.000 description 6
- 102000043136 MAP kinase family Human genes 0.000 description 5
- 108091054455 MAP kinase family Proteins 0.000 description 5
- 229940035893 uracil Drugs 0.000 description 5
- 239000012634 fragment Substances 0.000 description 4
- 235000015097 nutrients Nutrition 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 108091008146 restriction endonucleases Proteins 0.000 description 4
- 239000007787 solid Substances 0.000 description 4
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 230000007613 environmental effect Effects 0.000 description 3
- 239000013613 expression plasmid Substances 0.000 description 3
- 239000013604 expression vector Substances 0.000 description 3
- 239000012530 fluid Substances 0.000 description 3
- 239000008103 glucose Substances 0.000 description 3
- 230000012010 growth Effects 0.000 description 3
- 239000003921 oil Substances 0.000 description 3
- 230000003204 osmotic effect Effects 0.000 description 3
- 238000012216 screening Methods 0.000 description 3
- 238000012163 sequencing technique Methods 0.000 description 3
- 239000011780 sodium chloride Substances 0.000 description 3
- 230000009182 swimming Effects 0.000 description 3
- 230000009466 transformation Effects 0.000 description 3
- 241000222122 Candida albicans Species 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 241000588724 Escherichia coli Species 0.000 description 2
- 241000206602 Eukaryota Species 0.000 description 2
- 102000004232 Mitogen-Activated Protein Kinase Kinases Human genes 0.000 description 2
- 108090000744 Mitogen-Activated Protein Kinase Kinases Proteins 0.000 description 2
- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 2
- 229960000723 ampicillin Drugs 0.000 description 2
- 230000003321 amplification Effects 0.000 description 2
- 238000000137 annealing Methods 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 230000004087 circulation Effects 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000006870 function Effects 0.000 description 2
- 230000002068 genetic effect Effects 0.000 description 2
- 239000004519 grease Substances 0.000 description 2
- 238000003384 imaging method Methods 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000007246 mechanism Effects 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 238000003199 nucleic acid amplification method Methods 0.000 description 2
- 230000001717 pathogenic effect Effects 0.000 description 2
- 108010051242 phenylalanylserine Proteins 0.000 description 2
- 108010053725 prolylvaline Proteins 0.000 description 2
- 241000894007 species Species 0.000 description 2
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 1
- 229920000936 Agarose Polymers 0.000 description 1
- ZVFVBBGVOILKPO-WHFBIAKZSA-N Ala-Gly-Ala Chemical compound C[C@H](N)C(=O)NCC(=O)N[C@@H](C)C(O)=O ZVFVBBGVOILKPO-WHFBIAKZSA-N 0.000 description 1
- RZZMZYZXNJRPOJ-BJDJZHNGSA-N Ala-Ile-Lys Chemical compound CC[C@H](C)[C@@H](C(=O)N[C@@H](CCCCN)C(=O)O)NC(=O)[C@H](C)N RZZMZYZXNJRPOJ-BJDJZHNGSA-N 0.000 description 1
- LDLSENBXQNDTPB-DCAQKATOSA-N Ala-Lys-Arg Chemical compound NCCCC[C@H](NC(=O)[C@@H](N)C)C(=O)N[C@H](C(O)=O)CCCN=C(N)N LDLSENBXQNDTPB-DCAQKATOSA-N 0.000 description 1
- DHBKYZYFEXXUAK-ONGXEEELSA-N Ala-Phe-Gly Chemical compound OC(=O)CNC(=O)[C@@H](NC(=O)[C@@H](N)C)CC1=CC=CC=C1 DHBKYZYFEXXUAK-ONGXEEELSA-N 0.000 description 1
- FEGOCLZUJUFCHP-CIUDSAMLSA-N Ala-Pro-Gln Chemical compound [H]N[C@@H](C)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCC(N)=O)C(O)=O FEGOCLZUJUFCHP-CIUDSAMLSA-N 0.000 description 1
- IORKCNUBHNIMKY-CIUDSAMLSA-N Ala-Pro-Glu Chemical compound C[C@H](N)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCC(O)=O)C(O)=O IORKCNUBHNIMKY-CIUDSAMLSA-N 0.000 description 1
- HCBKAOZYACJUEF-XQXXSGGOSA-N Ala-Thr-Gln Chemical compound N[C@@H](C)C(=O)N[C@@H]([C@H](O)C)C(=O)N[C@@H](CCC(N)=O)C(=O)O HCBKAOZYACJUEF-XQXXSGGOSA-N 0.000 description 1
- BVLPIIBTWIYOML-ZKWXMUAHSA-N Ala-Val-Asp Chemical compound [H]N[C@@H](C)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC(O)=O)C(O)=O BVLPIIBTWIYOML-ZKWXMUAHSA-N 0.000 description 1
- 101100423825 Arabidopsis thaliana SYP61 gene Proteins 0.000 description 1
- QAODJPUKWNNNRP-DCAQKATOSA-N Arg-Glu-Arg Chemical compound NC(N)=NCCC[C@H](N)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCCN=C(N)N)C(O)=O QAODJPUKWNNNRP-DCAQKATOSA-N 0.000 description 1
- YKBHOXLMMPZPHQ-GMOBBJLQSA-N Arg-Ile-Asp Chemical compound [H]N[C@@H](CCCNC(N)=N)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CC(O)=O)C(O)=O YKBHOXLMMPZPHQ-GMOBBJLQSA-N 0.000 description 1
- ZDBWKBCKYJGKGP-DCAQKATOSA-N Arg-Leu-Ala Chemical compound [H]N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C)C(O)=O ZDBWKBCKYJGKGP-DCAQKATOSA-N 0.000 description 1
- QPTAGIPWARILES-AVGNSLFASA-N Asn-Gln-Phe Chemical compound [H]N[C@@H](CC(N)=O)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC1=CC=CC=C1)C(O)=O QPTAGIPWARILES-AVGNSLFASA-N 0.000 description 1
- XVAPVJNJGLWGCS-ACZMJKKPSA-N Asn-Glu-Asn Chemical compound C(CC(=O)O)[C@@H](C(=O)N[C@@H](CC(=O)N)C(=O)O)NC(=O)[C@H](CC(=O)N)N XVAPVJNJGLWGCS-ACZMJKKPSA-N 0.000 description 1
- ZEDBMCPXPIYJLW-XHNCKOQMSA-N Asp-Glu-Pro Chemical compound C1C[C@@H](N(C1)C(=O)[C@H](CCC(=O)O)NC(=O)[C@H](CC(=O)O)N)C(=O)O ZEDBMCPXPIYJLW-XHNCKOQMSA-N 0.000 description 1
- PYXXJFRXIYAESU-PCBIJLKTSA-N Asp-Ile-Phe Chemical compound [H]N[C@@H](CC(O)=O)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CC1=CC=CC=C1)C(O)=O PYXXJFRXIYAESU-PCBIJLKTSA-N 0.000 description 1
- CJUKAWUWBZCTDQ-SRVKXCTJSA-N Asp-Leu-Lys Chemical compound OC(=O)C[C@H](N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(O)=O CJUKAWUWBZCTDQ-SRVKXCTJSA-N 0.000 description 1
- HJZLUGQGJWXJCJ-CIUDSAMLSA-N Asp-Pro-Gln Chemical compound [H]N[C@@H](CC(O)=O)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCC(N)=O)C(O)=O HJZLUGQGJWXJCJ-CIUDSAMLSA-N 0.000 description 1
- RVMXMLSYBTXCAV-VEVYYDQMSA-N Asp-Pro-Thr Chemical compound [H]N[C@@H](CC(O)=O)C(=O)N1CCC[C@H]1C(=O)N[C@@H]([C@@H](C)O)C(O)=O RVMXMLSYBTXCAV-VEVYYDQMSA-N 0.000 description 1
- BOXNGMVEVOGXOJ-UBHSHLNASA-N Asp-Trp-Ser Chemical compound C1=CC=C2C(=C1)C(=CN2)C[C@@H](C(=O)N[C@@H](CO)C(=O)O)NC(=O)[C@H](CC(=O)O)N BOXNGMVEVOGXOJ-UBHSHLNASA-N 0.000 description 1
- 241001225321 Aspergillus fumigatus Species 0.000 description 1
- 241000972773 Aulopiformes Species 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- XABFFGOGKOORCG-CIUDSAMLSA-N Cys-Asp-Leu Chemical compound [H]N[C@@H](CS)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(C)C)C(O)=O XABFFGOGKOORCG-CIUDSAMLSA-N 0.000 description 1
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 description 1
- 108010005054 Deoxyribonuclease BamHI Proteins 0.000 description 1
- 108010042407 Endonucleases Proteins 0.000 description 1
- 102000004533 Endonucleases Human genes 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108020004460 Fungal RNA Proteins 0.000 description 1
- 206010064571 Gene mutation Diseases 0.000 description 1
- RWCBJYUPAUTWJD-NHCYSSNCSA-N Gln-Met-Val Chemical compound [H]N[C@@H](CCC(N)=O)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](C(C)C)C(O)=O RWCBJYUPAUTWJD-NHCYSSNCSA-N 0.000 description 1
- WHVLABLIJYGVEK-QEWYBTABSA-N Gln-Phe-Ile Chemical compound [H]N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC1=CC=CC=C1)C(=O)N[C@@H]([C@@H](C)CC)C(O)=O WHVLABLIJYGVEK-QEWYBTABSA-N 0.000 description 1
- YPFFHGRJCUBXPX-NHCYSSNCSA-N Gln-Pro-Val Chemical compound CC(C)[C@H](NC(=O)[C@@H]1CCCN1C(=O)[C@@H](N)CCC(N)=O)C(O)=O YPFFHGRJCUBXPX-NHCYSSNCSA-N 0.000 description 1
- ATRHMOJQJWPVBQ-DRZSPHRISA-N Glu-Ala-Phe Chemical compound [H]N[C@@H](CCC(O)=O)C(=O)N[C@@H](C)C(=O)N[C@@H](CC1=CC=CC=C1)C(O)=O ATRHMOJQJWPVBQ-DRZSPHRISA-N 0.000 description 1
- VAZZOGXDUQSVQF-NUMRIWBASA-N Glu-Asn-Thr Chemical compound C[C@H]([C@@H](C(=O)O)NC(=O)[C@H](CC(=O)N)NC(=O)[C@H](CCC(=O)O)N)O VAZZOGXDUQSVQF-NUMRIWBASA-N 0.000 description 1
- DSPQRJXOIXHOHK-WDSKDSINSA-N Glu-Asp-Gly Chemical compound OC(=O)CC[C@H](N)C(=O)N[C@@H](CC(O)=O)C(=O)NCC(O)=O DSPQRJXOIXHOHK-WDSKDSINSA-N 0.000 description 1
- IVGJYOOGJLFKQE-AVGNSLFASA-N Glu-Leu-Lys Chemical compound CC(C)C[C@@H](C(=O)N[C@@H](CCCCN)C(=O)O)NC(=O)[C@H](CCC(=O)O)N IVGJYOOGJLFKQE-AVGNSLFASA-N 0.000 description 1
- UUTGYDAKPISJAO-JYJNAYRXSA-N Glu-Tyr-Leu Chemical compound OC(=O)CC[C@H](N)C(=O)N[C@H](C(=O)N[C@@H](CC(C)C)C(O)=O)CC1=CC=C(O)C=C1 UUTGYDAKPISJAO-JYJNAYRXSA-N 0.000 description 1
- FGGKGJHCVMYGCD-UKJIMTQDSA-N Glu-Val-Ile Chemical compound [H]N[C@@H](CCC(O)=O)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H]([C@@H](C)CC)C(O)=O FGGKGJHCVMYGCD-UKJIMTQDSA-N 0.000 description 1
- VXKCPBPQEKKERH-IUCAKERBSA-N Gly-Arg-Pro Chemical compound NC(N)=NCCC[C@H](NC(=O)CN)C(=O)N1CCC[C@H]1C(O)=O VXKCPBPQEKKERH-IUCAKERBSA-N 0.000 description 1
- KKBWDNZXYLGJEY-UHFFFAOYSA-N Gly-Arg-Pro Natural products NCC(=O)NC(CCNC(=N)N)C(=O)N1CCCC1C(=O)O KKBWDNZXYLGJEY-UHFFFAOYSA-N 0.000 description 1
- LEGMTEAZGRRIMY-ZKWXMUAHSA-N Gly-Cys-Ile Chemical compound CC[C@H](C)[C@@H](C(=O)O)NC(=O)[C@H](CS)NC(=O)CN LEGMTEAZGRRIMY-ZKWXMUAHSA-N 0.000 description 1
- PAWIVEIWWYGBAM-YUMQZZPRSA-N Gly-Leu-Ala Chemical compound NCC(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C)C(O)=O PAWIVEIWWYGBAM-YUMQZZPRSA-N 0.000 description 1
- LOEANKRDMMVOGZ-YUMQZZPRSA-N Gly-Lys-Asp Chemical compound NCCCC[C@H](NC(=O)CN)C(=O)N[C@@H](CC(O)=O)C(O)=O LOEANKRDMMVOGZ-YUMQZZPRSA-N 0.000 description 1
- HHRODZSXDXMUHS-LURJTMIESA-N Gly-Met-Gly Chemical compound CSCC[C@H](NC(=O)C[NH3+])C(=O)NCC([O-])=O HHRODZSXDXMUHS-LURJTMIESA-N 0.000 description 1
- BMWFDYIYBAFROD-WPRPVWTQSA-N Gly-Pro-Val Chemical compound CC(C)[C@@H](C(O)=O)NC(=O)[C@@H]1CCCN1C(=O)CN BMWFDYIYBAFROD-WPRPVWTQSA-N 0.000 description 1
- NVTPVQLIZCOJFK-FOHZUACHSA-N Gly-Thr-Asp Chemical compound [H]NCC(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC(O)=O)C(O)=O NVTPVQLIZCOJFK-FOHZUACHSA-N 0.000 description 1
- 102100036669 Glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic Human genes 0.000 description 1
- 101150096276 HOG1 gene Proteins 0.000 description 1
- TVRMJKNELJKNRS-GUBZILKMSA-N His-Glu-Asn Chemical compound C1=C(NC=N1)C[C@@H](C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H](CC(=O)N)C(=O)O)N TVRMJKNELJKNRS-GUBZILKMSA-N 0.000 description 1
- SYIPVNMWBZXKMU-HJPIBITLSA-N His-His-Ile Chemical compound CC[C@H](C)[C@@H](C(=O)O)NC(=O)[C@H](CC1=CN=CN1)NC(=O)[C@H](CC2=CN=CN2)N SYIPVNMWBZXKMU-HJPIBITLSA-N 0.000 description 1
- WJGSTIMGSIWHJX-HVTMNAMFSA-N His-Ile-Gln Chemical compound CC[C@H](C)[C@@H](C(=O)N[C@@H](CCC(=O)N)C(=O)O)NC(=O)[C@H](CC1=CN=CN1)N WJGSTIMGSIWHJX-HVTMNAMFSA-N 0.000 description 1
- 101001072574 Homo sapiens Glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic Proteins 0.000 description 1
- DURWCDDDAWVPOP-JBDRJPRFSA-N Ile-Cys-Ser Chemical compound CC[C@H](C)[C@@H](C(=O)N[C@@H](CS)C(=O)N[C@@H](CO)C(=O)O)N DURWCDDDAWVPOP-JBDRJPRFSA-N 0.000 description 1
- DSDPLOODKXISDT-XUXIUFHCSA-N Ile-Leu-Val Chemical compound CC[C@H](C)[C@H](N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C(C)C)C(O)=O DSDPLOODKXISDT-XUXIUFHCSA-N 0.000 description 1
- WSSGUVAKYCQSCT-XUXIUFHCSA-N Ile-Met-Leu Chemical compound CC[C@H](C)[C@@H](C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CC(C)C)C(=O)O)N WSSGUVAKYCQSCT-XUXIUFHCSA-N 0.000 description 1
- JNLSTRPWUXOORL-MMWGEVLESA-N Ile-Ser-Pro Chemical compound CC[C@H](C)[C@@H](C(=O)N[C@@H](CO)C(=O)N1CCC[C@@H]1C(=O)O)N JNLSTRPWUXOORL-MMWGEVLESA-N 0.000 description 1
- YBHKCXNNNVDYEB-SPOWBLRKSA-N Ile-Trp-Ser Chemical compound CC[C@H](C)[C@@H](C(=O)N[C@@H](CC1=CNC2=CC=CC=C21)C(=O)N[C@@H](CO)C(=O)O)N YBHKCXNNNVDYEB-SPOWBLRKSA-N 0.000 description 1
- DZMWFIRHFFVBHS-ZEWNOJEFSA-N Ile-Tyr-Phe Chemical compound CC[C@H](C)[C@@H](C(=O)N[C@@H](CC1=CC=C(C=C1)O)C(=O)N[C@@H](CC2=CC=CC=C2)C(=O)O)N DZMWFIRHFFVBHS-ZEWNOJEFSA-N 0.000 description 1
- 102100039929 Intraflagellar transport protein 172 homolog Human genes 0.000 description 1
- SITWEMZOJNKJCH-UHFFFAOYSA-N L-alanine-L-arginine Natural products CC(N)C(=O)NC(C(O)=O)CCCNC(N)=N SITWEMZOJNKJCH-UHFFFAOYSA-N 0.000 description 1
- PBCHMHROGNUXMK-DLOVCJGASA-N Leu-Ala-His Chemical compound CC(C)C[C@H](N)C(=O)N[C@@H](C)C(=O)N[C@H](C(O)=O)CC1=CN=CN1 PBCHMHROGNUXMK-DLOVCJGASA-N 0.000 description 1
- VKOAHIRLIUESLU-ULQDDVLXSA-N Leu-Arg-Phe Chemical compound [H]N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC1=CC=CC=C1)C(O)=O VKOAHIRLIUESLU-ULQDDVLXSA-N 0.000 description 1
- CQGSYZCULZMEDE-UHFFFAOYSA-N Leu-Gln-Pro Natural products CC(C)CC(N)C(=O)NC(CCC(N)=O)C(=O)N1CCCC1C(O)=O CQGSYZCULZMEDE-UHFFFAOYSA-N 0.000 description 1
- HFBCHNRFRYLZNV-GUBZILKMSA-N Leu-Glu-Asp Chemical compound [H]N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(O)=O)C(O)=O HFBCHNRFRYLZNV-GUBZILKMSA-N 0.000 description 1
- HQUXQAMSWFIRET-AVGNSLFASA-N Leu-Glu-Lys Chemical compound CC(C)C[C@H](N)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@H](C(O)=O)CCCCN HQUXQAMSWFIRET-AVGNSLFASA-N 0.000 description 1
- HYMLKESRWLZDBR-WEDXCCLWSA-N Leu-Gly-Thr Chemical compound CC(C)C[C@H](N)C(=O)NCC(=O)N[C@@H]([C@@H](C)O)C(O)=O HYMLKESRWLZDBR-WEDXCCLWSA-N 0.000 description 1
- VZBIUJURDLFFOE-IHRRRGAJSA-N Leu-His-Arg Chemical compound [H]N[C@@H](CC(C)C)C(=O)N[C@@H](CC1=CNC=N1)C(=O)N[C@@H](CCCNC(N)=N)C(O)=O VZBIUJURDLFFOE-IHRRRGAJSA-N 0.000 description 1
- LXKNSJLSGPNHSK-KKUMJFAQSA-N Leu-Leu-Lys Chemical compound CC(C)C[C@@H](C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)O)N LXKNSJLSGPNHSK-KKUMJFAQSA-N 0.000 description 1
- IEWBEPKLKUXQBU-VOAKCMCISA-N Leu-Leu-Thr Chemical compound [H]N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H]([C@@H](C)O)C(O)=O IEWBEPKLKUXQBU-VOAKCMCISA-N 0.000 description 1
- UHNQRAFSEBGZFZ-YESZJQIVSA-N Leu-Phe-Pro Chemical compound CC(C)C[C@@H](C(=O)N[C@@H](CC1=CC=CC=C1)C(=O)N2CCC[C@@H]2C(=O)O)N UHNQRAFSEBGZFZ-YESZJQIVSA-N 0.000 description 1
- YRRCOJOXAJNSAX-IHRRRGAJSA-N Leu-Pro-Lys Chemical compound CC(C)C[C@@H](C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCCCN)C(=O)O)N YRRCOJOXAJNSAX-IHRRRGAJSA-N 0.000 description 1
- MVHXGBZUJLWZOH-BJDJZHNGSA-N Leu-Ser-Ile Chemical compound [H]N[C@@H](CC(C)C)C(=O)N[C@@H](CO)C(=O)N[C@@H]([C@@H](C)CC)C(O)=O MVHXGBZUJLWZOH-BJDJZHNGSA-N 0.000 description 1
- VDIARPPNADFEAV-WEDXCCLWSA-N Leu-Thr-Gly Chemical compound CC(C)C[C@H](N)C(=O)N[C@@H]([C@@H](C)O)C(=O)NCC(O)=O VDIARPPNADFEAV-WEDXCCLWSA-N 0.000 description 1
- TUIOUEWKFFVNLH-DCAQKATOSA-N Leu-Val-Cys Chemical compound CC(C)C[C@H](N)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CS)C(O)=O TUIOUEWKFFVNLH-DCAQKATOSA-N 0.000 description 1
- XDPLZVNMYQOFQZ-BJDJZHNGSA-N Lys-Ile-Cys Chemical compound CC[C@H](C)[C@@H](C(=O)N[C@@H](CS)C(=O)O)NC(=O)[C@H](CCCCN)N XDPLZVNMYQOFQZ-BJDJZHNGSA-N 0.000 description 1
- CBNMHRCLYBJIIZ-XUXIUFHCSA-N Lys-Ile-Met Chemical compound CC[C@H](C)[C@@H](C(=O)N[C@@H](CCSC)C(=O)O)NC(=O)[C@H](CCCCN)N CBNMHRCLYBJIIZ-XUXIUFHCSA-N 0.000 description 1
- LECIJRIRMVOFMH-ULQDDVLXSA-N Lys-Pro-Phe Chemical compound NCCCC[C@H](N)C(=O)N1CCC[C@H]1C(=O)N[C@H](C(O)=O)CC1=CC=CC=C1 LECIJRIRMVOFMH-ULQDDVLXSA-N 0.000 description 1
- RMKJOQSYLQQRFN-KKUMJFAQSA-N Lys-Tyr-Asp Chemical compound [H]N[C@@H](CCCCN)C(=O)N[C@@H](CC1=CC=C(O)C=C1)C(=O)N[C@@H](CC(O)=O)C(O)=O RMKJOQSYLQQRFN-KKUMJFAQSA-N 0.000 description 1
- 102000001291 MAP Kinase Kinase Kinase Human genes 0.000 description 1
- 108060006687 MAP kinase kinase kinase Proteins 0.000 description 1
- 241001344131 Magnaporthe grisea Species 0.000 description 1
- VHGIWFGJIHTASW-FXQIFTODSA-N Met-Ala-Asp Chemical compound CSCC[C@H](N)C(=O)N[C@@H](C)C(=O)N[C@H](C(O)=O)CC(O)=O VHGIWFGJIHTASW-FXQIFTODSA-N 0.000 description 1
- HGAJNEWOUHDUMZ-SRVKXCTJSA-N Met-Leu-Glu Chemical compound CSCC[C@H](N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@H](C(O)=O)CCC(O)=O HGAJNEWOUHDUMZ-SRVKXCTJSA-N 0.000 description 1
- CIIJWIAORKTXAH-FJXKBIBVSA-N Met-Thr-Gly Chemical compound CSCC[C@H](N)C(=O)N[C@@H]([C@@H](C)O)C(=O)NCC(O)=O CIIJWIAORKTXAH-FJXKBIBVSA-N 0.000 description 1
- YBAFDPFAUTYYRW-UHFFFAOYSA-N N-L-alpha-glutamyl-L-leucine Natural products CC(C)CC(C(O)=O)NC(=O)C(N)CCC(O)=O YBAFDPFAUTYYRW-UHFFFAOYSA-N 0.000 description 1
- SITLTJHOQZFJGG-UHFFFAOYSA-N N-L-alpha-glutamyl-L-valine Natural products CC(C)C(C(O)=O)NC(=O)C(N)CCC(O)=O SITLTJHOQZFJGG-UHFFFAOYSA-N 0.000 description 1
- CYZBFPYMSJGBRL-DRZSPHRISA-N Phe-Ala-Glu Chemical compound [H]N[C@@H](CC1=CC=CC=C1)C(=O)N[C@@H](C)C(=O)N[C@@H](CCC(O)=O)C(O)=O CYZBFPYMSJGBRL-DRZSPHRISA-N 0.000 description 1
- QPVFUAUFEBPIPT-CDMKHQONSA-N Phe-Gly-Thr Chemical compound [H]N[C@@H](CC1=CC=CC=C1)C(=O)NCC(=O)N[C@@H]([C@@H](C)O)C(O)=O QPVFUAUFEBPIPT-CDMKHQONSA-N 0.000 description 1
- RGMLUHANLDVMPB-ULQDDVLXSA-N Phe-Val-Lys Chemical compound CC(C)[C@@H](C(=O)N[C@@H](CCCCN)C(=O)O)NC(=O)[C@H](CC1=CC=CC=C1)N RGMLUHANLDVMPB-ULQDDVLXSA-N 0.000 description 1
- 108091000080 Phosphotransferase Proteins 0.000 description 1
- QSKCKTUQPICLSO-AVGNSLFASA-N Pro-Arg-Lys Chemical compound C1C[C@H](NC1)C(=O)N[C@@H](CCCN=C(N)N)C(=O)N[C@@H](CCCCN)C(=O)O QSKCKTUQPICLSO-AVGNSLFASA-N 0.000 description 1
- FHZJRBVMLGOHBX-GUBZILKMSA-N Pro-Pro-Asp Chemical compound OC(=O)C[C@H](NC(=O)[C@@H]1CCCN1C(=O)[C@@H]1CCCN1)C(O)=O FHZJRBVMLGOHBX-GUBZILKMSA-N 0.000 description 1
- OWQXAJQZLWHPBH-FXQIFTODSA-N Pro-Ser-Asn Chemical compound [H]N1CCC[C@H]1C(=O)N[C@@H](CO)C(=O)N[C@@H](CC(N)=O)C(O)=O OWQXAJQZLWHPBH-FXQIFTODSA-N 0.000 description 1
- SHTKRJHDMNSKRM-ULQDDVLXSA-N Pro-Tyr-His Chemical compound C1C[C@H](NC1)C(=O)N[C@@H](CC2=CC=C(C=C2)O)C(=O)N[C@@H](CC3=CN=CN3)C(=O)O SHTKRJHDMNSKRM-ULQDDVLXSA-N 0.000 description 1
- 238000012181 QIAquick gel extraction kit Methods 0.000 description 1
- MUPFEKGTMRGPLJ-RMMQSMQOSA-N Raffinose Natural products O(C[C@H]1[C@@H](O)[C@H](O)[C@@H](O)[C@@H](O[C@@]2(CO)[C@H](O)[C@@H](O)[C@@H](CO)O2)O1)[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 MUPFEKGTMRGPLJ-RMMQSMQOSA-N 0.000 description 1
- 101100393304 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GPD1 gene Proteins 0.000 description 1
- WTUJZHKANPDPIN-CIUDSAMLSA-N Ser-Ala-Lys Chemical compound C[C@@H](C(=O)N[C@@H](CCCCN)C(=O)O)NC(=O)[C@H](CO)N WTUJZHKANPDPIN-CIUDSAMLSA-N 0.000 description 1
- NRCJWSGXMAPYQX-LPEHRKFASA-N Ser-Arg-Pro Chemical compound C1C[C@@H](N(C1)C(=O)[C@H](CCCN=C(N)N)NC(=O)[C@H](CO)N)C(=O)O NRCJWSGXMAPYQX-LPEHRKFASA-N 0.000 description 1
- HBTCFCHYALPXME-HTFCKZLJSA-N Ser-Ile-Ile Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H]([C@@H](C)CC)C(O)=O HBTCFCHYALPXME-HTFCKZLJSA-N 0.000 description 1
- YUJLIIRMIAGMCQ-CIUDSAMLSA-N Ser-Leu-Ser Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CO)C(O)=O YUJLIIRMIAGMCQ-CIUDSAMLSA-N 0.000 description 1
- ZSDXEKUKQAKZFE-XAVMHZPKSA-N Ser-Thr-Pro Chemical compound C[C@H]([C@@H](C(=O)N1CCC[C@@H]1C(=O)O)NC(=O)[C@H](CO)N)O ZSDXEKUKQAKZFE-XAVMHZPKSA-N 0.000 description 1
- UNURFMVMXLENAZ-KJEVXHAQSA-N Thr-Arg-Tyr Chemical compound [H]N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC1=CC=C(O)C=C1)C(O)=O UNURFMVMXLENAZ-KJEVXHAQSA-N 0.000 description 1
- LHEZGZQRLDBSRR-WDCWCFNPSA-N Thr-Glu-Leu Chemical compound [H]N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(C)C)C(O)=O LHEZGZQRLDBSRR-WDCWCFNPSA-N 0.000 description 1
- ONNSECRQFSTMCC-XKBZYTNZSA-N Thr-Glu-Ser Chemical compound [H]N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CO)C(O)=O ONNSECRQFSTMCC-XKBZYTNZSA-N 0.000 description 1
- SOUPNXUJAJENFU-SWRJLBSHSA-N Thr-Trp-Gln Chemical compound C[C@H]([C@@H](C(=O)N[C@@H](CC1=CNC2=CC=CC=C21)C(=O)N[C@@H](CCC(=O)N)C(=O)O)N)O SOUPNXUJAJENFU-SWRJLBSHSA-N 0.000 description 1
- BEZTUFWTPVOROW-KJEVXHAQSA-N Thr-Tyr-Arg Chemical compound C[C@H]([C@@H](C(=O)N[C@@H](CC1=CC=C(C=C1)O)C(=O)N[C@@H](CCCN=C(N)N)C(=O)O)N)O BEZTUFWTPVOROW-KJEVXHAQSA-N 0.000 description 1
- RVGVIWNHABGIFH-IHRRRGAJSA-N Tyr-Val-Ser Chemical compound [H]N[C@@H](CC1=CC=C(O)C=C1)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CO)C(O)=O RVGVIWNHABGIFH-IHRRRGAJSA-N 0.000 description 1
- MUPFEKGTMRGPLJ-UHFFFAOYSA-N UNPD196149 Natural products OC1C(O)C(CO)OC1(CO)OC1C(O)C(O)C(O)C(COC2C(C(O)C(O)C(CO)O2)O)O1 MUPFEKGTMRGPLJ-UHFFFAOYSA-N 0.000 description 1
- ZLFHAAGHGQBQQN-AEJSXWLSSA-N Val-Ala-Pro Chemical compound C[C@@H](C(=O)N1CCC[C@@H]1C(=O)O)NC(=O)[C@H](C(C)C)N ZLFHAAGHGQBQQN-AEJSXWLSSA-N 0.000 description 1
- ZLFHAAGHGQBQQN-GUBZILKMSA-N Val-Ala-Pro Natural products CC(C)[C@H](N)C(=O)N[C@@H](C)C(=O)N1CCC[C@H]1C(O)=O ZLFHAAGHGQBQQN-GUBZILKMSA-N 0.000 description 1
- TZVUSFMQWPWHON-NHCYSSNCSA-N Val-Asp-Leu Chemical compound CC(C)C[C@@H](C(=O)O)NC(=O)[C@H](CC(=O)O)NC(=O)[C@H](C(C)C)N TZVUSFMQWPWHON-NHCYSSNCSA-N 0.000 description 1
- PWRITNSESKQTPW-NRPADANISA-N Val-Gln-Ser Chemical compound CC(C)[C@@H](C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H](CO)C(=O)O)N PWRITNSESKQTPW-NRPADANISA-N 0.000 description 1
- WMRWZYSRQUORHJ-YDHLFZDLSA-N Val-Phe-Asp Chemical compound CC(C)[C@@H](C(=O)N[C@@H](CC1=CC=CC=C1)C(=O)N[C@@H](CC(=O)O)C(=O)O)N WMRWZYSRQUORHJ-YDHLFZDLSA-N 0.000 description 1
- YLRAFVVWZRSZQC-DZKIICNBSA-N Val-Phe-Glu Chemical compound CC(C)[C@@H](C(=O)N[C@@H](CC1=CC=CC=C1)C(=O)N[C@@H](CCC(=O)O)C(=O)O)N YLRAFVVWZRSZQC-DZKIICNBSA-N 0.000 description 1
- UVHFONIHVHLDDQ-IFFSRLJSSA-N Val-Thr-Glu Chemical compound C[C@H]([C@@H](C(=O)N[C@@H](CCC(=O)O)C(=O)O)NC(=O)[C@H](C(C)C)N)O UVHFONIHVHLDDQ-IFFSRLJSSA-N 0.000 description 1
- JAIZPWVHPQRYOU-ZJDVBMNYSA-N Val-Thr-Thr Chemical compound C[C@H]([C@@H](C(=O)N[C@@H]([C@@H](C)O)C(=O)O)NC(=O)[C@H](C(C)C)N)O JAIZPWVHPQRYOU-ZJDVBMNYSA-N 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 108010041407 alanylaspartic acid Proteins 0.000 description 1
- 150000001413 amino acids Chemical group 0.000 description 1
- 108010013835 arginine glutamate Proteins 0.000 description 1
- 108010001271 arginyl-glutamyl-arginine Proteins 0.000 description 1
- 108010069205 aspartyl-phenylalanine Proteins 0.000 description 1
- 108010093581 aspartyl-proline Proteins 0.000 description 1
- 108010038633 aspartylglutamate Proteins 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000031018 biological processes and functions Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 238000010805 cDNA synthesis kit Methods 0.000 description 1
- 229940095731 candida albicans Drugs 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 235000019504 cigarettes Nutrition 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 239000002299 complementary DNA Substances 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 230000010429 evolutionary process Effects 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 244000000004 fungal plant pathogen Species 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 108010042598 glutamyl-aspartyl-glycine Proteins 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- VPZXBVLAVMBEQI-UHFFFAOYSA-N glycyl-DL-alpha-alanine Natural products OC(=O)C(C)NC(=O)CN VPZXBVLAVMBEQI-UHFFFAOYSA-N 0.000 description 1
- XBGGUPMXALFZOT-UHFFFAOYSA-N glycyl-L-tyrosine hemihydrate Natural products NCC(=O)NC(C(O)=O)CC1=CC=C(O)C=C1 XBGGUPMXALFZOT-UHFFFAOYSA-N 0.000 description 1
- 108010050848 glycylleucine Proteins 0.000 description 1
- 239000003262 industrial enzyme Substances 0.000 description 1
- 230000008595 infiltration Effects 0.000 description 1
- 238000001764 infiltration Methods 0.000 description 1
- 230000007154 intracellular accumulation Effects 0.000 description 1
- 101150044508 key gene Proteins 0.000 description 1
- 108010034529 leucyl-lysine Proteins 0.000 description 1
- 108010090333 leucyl-lysyl-proline Proteins 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- XIXADJRWDQXREU-UHFFFAOYSA-M lithium acetate Chemical compound [Li+].CC([O-])=O XIXADJRWDQXREU-UHFFFAOYSA-M 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 108010003700 lysyl aspartic acid Proteins 0.000 description 1
- 108010054155 lysyllysine Proteins 0.000 description 1
- 230000010534 mechanism of action Effects 0.000 description 1
- 230000001404 mediated effect Effects 0.000 description 1
- HEBKCHPVOIAQTA-UHFFFAOYSA-N meso ribitol Natural products OCC(O)C(O)C(O)CO HEBKCHPVOIAQTA-UHFFFAOYSA-N 0.000 description 1
- 108010022588 methionyl-lysyl-proline Proteins 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 239000003226 mitogen Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 230000004660 morphological change Effects 0.000 description 1
- 101150078678 osm-1 gene Proteins 0.000 description 1
- 230000037361 pathway Effects 0.000 description 1
- 102000020233 phosphotransferase Human genes 0.000 description 1
- 230000004962 physiological condition Effects 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108010029020 prolylglycine Proteins 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- MUPFEKGTMRGPLJ-ZQSKZDJDSA-N raffinose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO[C@@H]2[C@@H]([C@@H](O)[C@@H](O)[C@@H](CO)O2)O)O1 MUPFEKGTMRGPLJ-ZQSKZDJDSA-N 0.000 description 1
- 230000006798 recombination Effects 0.000 description 1
- 238000005215 recombination Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 230000008844 regulatory mechanism Effects 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 238000010839 reverse transcription Methods 0.000 description 1
- 235000019515 salmon Nutrition 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 230000011664 signaling Effects 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- 239000000600 sorbitol Substances 0.000 description 1
- 150000005846 sugar alcohols Polymers 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 230000035897 transcription Effects 0.000 description 1
- 238000013518 transcription Methods 0.000 description 1
- 238000010361 transduction Methods 0.000 description 1
- 230000026683 transduction Effects 0.000 description 1
- 108010035534 tyrosyl-leucyl-alanine Proteins 0.000 description 1
- 108010003137 tyrosyltyrosine Proteins 0.000 description 1
- 239000013598 vector Substances 0.000 description 1
- 230000001018 virulence Effects 0.000 description 1
- -1 β-carotenoid Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
Abstract
本发明公开了一种高渗透压甘油蛋白激酶基因RkHog1及其重组表达载体，其核苷酸序列如SEQ ID NO：1所示，该基因编码的氨基酸序列如SEQ ID NO：2所示；该基因是酵母中参与耐高渗透压调控的功能基因，有助于酵母在高渗透压和高盐等环境中生长。
Description
技术领域
本发明属于生物技术领域和遗传工程领域，涉及一种高渗透压甘油蛋白激酶基因RkHog1及其重组表达载体。
背景技术
生物在长期的进化过程中形成了精确而敏感的适应机制，对外界环境刺激作出应答反应。有丝分裂原活化蛋白激酶(Mitogen-activated protein kinase，MAPK)是一类普遍存在于真核生物且在进化上保守的蛋白激酶，主要参与传导胞外信号，调节细胞的多种生物学过程。MAPK通常由MAPKK激活，而MAPKK又受到MAPKKK的活化。高渗透压甘油促分裂原激酶信号转导途径HOG-MAPK(high osmohrity glycerol mitogen-activated proteinkinase signaling transduction pathway)是酿酒酵母对外界高渗透压胁迫环境应答的重要途径之一。该途径通过促进甘油积累、阻遏细胞生长及其他生理条件调节来抵抗渗透胁迫刺激。几乎所有真核生物的HOG-MAPK 途径作用均是高渗透压环境中调控胞内生理生化反应以快速合成和/或积累胞内甘油提高胞内渗透压。
HOG信号途径首先发现于酿酒酵母，该信号途径受到外界高渗条件的诱导，激活甘油合成相关酶基因（如 GPD1 和 HOR1）的转录，使胞内积累高浓度的甘油，维持细胞较高的膨压。近年来的研究表明，该信号途径广泛存在于其它丝状真菌，与真菌胞内的膨压调节密切相关。但在不同的真菌中，HOG-MAPK信号途径调节胞内小分子量多元醇的种类不同。如人类病原真菌(Candida albicans) 在高渗环境中，HOG-MAPK主要通过调控甘油的积累提高胞内膨压，抵御不良环境；植物病原真菌(Magnaporthe grisea) 中，当 HOG-MAPK 编码基因 OSM1 被破坏后，菌体在高渗条件下的形态表现异常，胞内阿拉伯糖醇的积累显著降低，而甘油积累却没有明显变化。研究还发现，蛋白激酶Hoglp与病原真菌的致病性有关。烟曲霉(A．fumigatus)OSMl与酵母HOGl基因同源，OSMl基因突变体在高渗条件下毒力下降，菌丝形态改变；白念珠(C．albicans)Hogl突变体菌丝形成受到抑制，突变株对小鼠的致死率显著下降。
近年来，HOG-MAPK途径的重要蛋白激酶Hoglp的编码基因已在多种丝状真菌中被分离
和研究。红冬孢酵母（Rhodosporidium kratochvilovae）作为优良的微生物油脂生产菌，在营养限制条件下能过量积累胞内油脂；并生产胞外多糖、β-类胡萝卜素、工业用酶等，在生物制药产业和环境治理方面中有广泛的应用前景。同时，红冬孢酵母具有生长周期短、利用碳源广泛、油脂积累量多等优点，还能在高渗透压环境中生长，因此，该菌具有适应不同环境的调节机制。然而，有关HOG信号途径与其环境适应性、生长发育、致病性等关系的研究较少。因此，克隆获得高渗透压甘油蛋白激酶基因RkHog1并进行功能研究，分析HOG信号途径在该菌中的作用，明确HOG信号途径的作用机理及其生长适应性的关系是为今后更好地利用及改造相关生物基因表达调控这一重要生物资源提供十分重要的手段，为利用基因工程技术改善微生物及其他高等真核生物的抗逆性改良提供了更加丰富的优良基因材料。
发明内容
本发明的目的是提供一种高渗透压甘油蛋白激酶基因RkHog1，这是从红冬孢酵母（Rhodosporidium kratochvilovae）YM25235中分离得到，该基因核苷酸序列如SEQ ID NO：1所示或该核苷酸序列的片段，或与SEQ ID NO：1互补的核苷酸序列，该基因序列长为1080bp（碱基），该基因编码的氨基酸序列如SEQ ID NO：2所示的多肽或其片段。
本发明另一目的是提供一种含有高渗透压甘油蛋白激酶基因RkHog1的重组表达载体，是将SEQ ID NO：1所示基因直接与不同表达载体（质粒、病毒或运载体）连接所构建的重组载体。
本发明另一目的是提供一种含有高渗透压甘油蛋白激酶基因RkHog1或上述重组表达载体的宿主细胞酿酒酵母BY4742。
具体涉及从红冬孢酵母（Rhodosporidium kratochvilovae）YM25235中克隆的高渗透压甘油蛋白激酶RkHog1基因及将该基因直接与不同载体连接，转入细菌、酵母或其他真菌中，并对其表达特性进行研究，为阐明HOG途径在红冬孢酵母YM25235中的功能奠定了基础。
本发明从红冬孢酵母（Rhodosporidium kratochvilovae）YM25235的染色体基因组中分离得到HOG途径的核心分子Hog1p的基因RkHog1。该基因全长1080bp，研究表明基因RkHog1功能与酿酒酵母Hog1完全相同，且性能更佳。该基因的获得扩大了微生物抗渗透压胁迫研究的基因资源，也为红冬孢酵母HOG信号途径的分子机理奠定基础，为今后更好地利用及改造相关生物基因表达调控这一重要生物资源提供十分重要的手段。该基因是耐高渗透压调控的功能基因，若将耐高渗的功能基因Hog1导入植物，就可以进行耐盐新品种的选育，又如利用 Hog1调控产甘油关键酶基因GPD高效启动子，可用于工业上产油菌株的改造；从而为培养耐高渗透压和高盐环境的生物新品种提供了新的材料和新的思路。
附图说明
图1为本发明的红冬孢酵母YM25235 RkHog1基因PCR扩增图；
图2为利用本发明的红冬孢酵母YM25235 RkHog1基因构建的酿酒酵母表达载体pW3RkHog1结构示意图；
图3为本发明所构建的重组表达质粒pW3RkHog1的酶切分析图；其中：1为DNA Maker；2为空质粒pWXY3.0双酶切对照；3为重组质粒pW3RkHog1双酶切；4为RkHog1双酶切对照；5 为DNA Maker ；
图4 为重组菌株在不同NaCl浓度的固体培养基上的菌落形态；A图为0.5MNaCl浓度，B图为1.0MNaCl浓度；图中从左到右分别为重组质粒pW3RkHog1转化的酿酒酵母Hog1缺失菌株、重组质粒pW3SCHog1转化的酿酒酵母Hog1缺失菌株、空质粒pWXY3.0转化的酿酒酵母Hog1缺失菌株、空质粒pWXY3.0转化的酿酒酵母BY4742野生菌株；
图5 为重组菌株在不同山梨醇浓度的固体培养基上的菌落形态；A图为1.2M浓度，B图为1.8浓度；图中从左到右分别为重组质粒pW3RkHog1转化的酿酒酵母Hog1缺失菌株、重组质粒pW3SCHog1转化的酿酒酵母Hog1缺失菌株、空质粒pWXY3.0转化的酿酒酵母Hog1缺失菌株、空质粒pWXY3.0转化的酿酒酵母BY4742野生菌株。
具体实施方式
下面结合附图和实施例对本发明作进一步详细说明，但本发明保护范围不局限于所述内容，实施例中使用的试剂和方法，如无特殊说明，均采用常规试剂和使用常规方法。
实施例1：红冬孢酵母（Rhodosporidium kratochvilovae）YM25235 RkHog1基因克隆
采用OMEGA试剂盒E.Z.N.A Fungal RNA Kit提取红冬孢酵母YM25235总RNA，反转录试剂盒Takara First Strand cDNA Synthesis Kit合成cDNA。根据红冬孢酵母YM25235的转录组序列设计特异性引物（引物1和引物2）进行PCR扩增。反应所用引物、组份和扩增条件如下：
引物1：RkHog1-F: 5’- CGCAAGCTTATGGCCGACTTCGTGAAG -3’ （SEQ ID NO:3）
引物2：RkHog1-R: 5’- TAATGGATCCTTACTGCTGCGGCGCG -3’ （SEQ ID NO:4）
（AAGCTT为HindⅢ酶切位点，GGATCC为BamHⅠ酶切位点）；
PCR扩增体系如下（50 μL）：
PCR扩增条件：95℃预变性5 min，再用95℃变性30 s，63℃退火15 s，72℃延伸1 min进行30个循环，最后72℃彻底延伸10 min。反应完后取产物2μL，然后在浓度为1%的琼脂糖凝胶中，进行电泳分析，结果如图1所示。经凝胶成像系统成像确认片段大小正确后，用百泰克生物技术有限公司多动能DNA纯化回收试剂盒回收目的片段，然后将PCR扩增得到的目的基因连接到pMD-18T上，连接产物转化至大肠杆菌DH5α感受态细胞中，用含有氨苄青霉素（AMP+）的LB固体平板进行筛选，挑取平板上的转化子进行菌落PCR筛选阳性克隆，然后送去上海生工测序。测序结果显示，获得一段1080 bp长的序列，命名为RkHog1，序列组成如SEQ IDNO:1所示的核苷酸序列。
实施例2：重组表达质粒pW3RKHog1的构建
采用实施例1中cDNA为模板进行PCR扩增，反应所有引物组合、反应组分和扩增条件如下：
引物1：RkHog1-F: 5’- CGCAAGCTTATGGCCGACTTCGTGAAG -3’ （SEQ ID NO:3）
引物2：RkHog1-R:5’- TAATGGATCCTTACTGCTGCGGCGCG -3’ （SEQ ID NO:4）
（AAGCTT为Hind Ⅲ酶切位点，GGATCC为BamHⅠ酶切位点）；
PCR扩增体系如下（50 μL）：
PCR扩增条件：95℃预变性5 min，再用95℃变性30 s，63℃退火15 s，72℃延伸1 min进行30个循环，最后72℃彻底延伸10 min。
将上述PCR获得的基因片段和表达载体pWXY3.0用Hind Ⅲ和BamHⅠ核酸内切酶进行双酶切，用百泰克生物技术有限公司多动能DNA纯化回收试剂盒回收酶切片段，并用T4连接酶在16℃连接过夜，连接产物转化大肠杆菌DH5α，用含有氨苄青霉素(Amp+)的LB固体平板进行筛选，挑取平板上的转化子进行菌落PCR，筛选阳性克隆，同时进一步对转化子进行双酶切验证分析。结果显示，如图3第3泳道所示，用Hind Ⅲ和BamHⅠ双酶切，重组质粒产生两条带，小分子条带与泳道4中该基因的PCR产物大小一致，大分子条带
与泳道2中用相同酶切质粒pWXY3.0后产生的条带大小一致，这表明所构建的重组质粒正确，进一步测序分析也证明这一点。将重组表达质粒命名为pW3RkHog1，该质粒图谱如图2所示。
实施例3：红冬孢酵母（Rhodosporidium kratochvilovae）YM25235RkHog1基因在提高酿酒酵母Hog1缺失菌株中耐高渗的应用
1、 重组质粒pW3RkHog1转化酿酒酵母Hog1缺失菌株BY4742
采用鲑鱼精DNA介导的醋酸锂化学转化法将重组质粒pW3RkHog1转化至酿酒酵母Hog1缺失菌株中，以尿嘧啶营养缺陷型（含2%葡萄糖）的培养基筛选转化子。菌落PCR验证转化子。
2、高渗条件下重组菌株的生长。
挑取空质粒转化的酿酒酵母BY4742野生菌株、空质粒转化的酿酒酵母Hog1缺失菌株、重组质粒pW3SCHog1转化的酿酒酵母Hog1缺失菌株和重组质粒pW3RkHog1转化的酿酒酵母Hog1缺失菌株单克隆于5 mL的尿嘧啶营养缺陷型（含2%葡萄糖）液体培养基中，于30℃，150 rpm培养24 h；分别将上步酵母菌液于4000 rpm下离心5 min收集菌体，并用尿嘧啶营养缺陷型液体培养基洗涤3次，于4000 rpm下离心5 min收集菌体，于尿嘧啶营养缺陷型（含2%半乳糖+2%棉籽糖）液体培养基，并确保其初始OD600值在0.3-0.6之间，在30℃，80 rpm下诱导12 h。分别将诱导后的菌液的OD600值调整为0.8，并同时用血球计数板计数（四个菌株之间的菌数尽量调整一致，以避免较大的误差）。将上步OD600值调整一致的菌液分别十倍稀释至10-1、10-2、10-3、10-4、10-5分别取2 µL点板于尿嘧啶营养缺陷型（含2%葡萄糖）0.5 MNaCl、1.0 M NaCl、1.2 M山梨醇、1.8 M山梨醇平板上，28℃倒置培养48 h。观察四个菌株菌落的差别（见图4和5）。(从左到右为重组质粒pW3RkHog1转化的酿酒酵母Hog1缺失菌株、重组质粒pW3SCHog1转化的酿酒酵母Hog1缺失菌株、空质粒pWXY3.0转化的酿酒酵母Hog1缺失菌株、空质粒pWXY3.0转化的酿酒酵母BY4742野生菌株)
通过RkHog1基因在酿酒酵母Hog1缺失菌株BY4742中互补表达可以看出，重组菌株BY4742- RkHog1相较于对照原始菌株BY4742耐高渗能力显著提高。
序列表
<110> 昆明理工大学
<120> 高渗透压甘油蛋白激酶基因RkHog1及其重组表达载体
<160> 4
<170> PatentIn version 3.3
<210> 1
<211> 1080
<212> DNA
<213> Rhodosporidium kratochvilovae
<400> 1
atggccgact tcgtgaagct cagcatcttt ggaaccgtct tcgaggtcac cacccggtat 60
gtcgacctcc agccggtcgg catgggcgcg ttcggactcg tctgctcggc caaggatgag 120
ctgacaggcc agatggtcgc gatcaagaag atcatgaagc cgttcagcac ccccgtcctc 180
gcaaagcgga cgtaccgcga gctcaagctc ttgaagcaca tccagcacga gaacatcatc 240
tcgctgtccg acatcttcat ctcccccctc gaggacatct actttgtcac cgagctgctc 300
ggtaccgacc tccaccgcct gttgacgagc cggccgctcg agaagcagtt catccagtac 360
ttcctctacc agattctccg tggcttgaag tacgtgcact cggcaggcgt tgtccaccgc 420
gacctcaagc cctccaacat cctcgtcaac gagaactgcg acctcaagat ctgcgacttt 480
ggcctcgcgc gcctcgcaga cccgcagatg accggctacg tctcgacgcg gtactaccgc 540
gcgcctgaga tcatgctcac gtggcagaag tacgacgttg ccgtcgacat ctggtccgcg 600
gggtgcatct tcgccgagat gctggagggc cgcccgctct tcccgggcaa ggaccacgtc 660
aaccagttct cgatcatcac cgagctgctg gggacgccgc ctgacgaggt catccagacc 720
atctgctcgg agaacaccct tcgcttcgtc cagtcgctcc cgaaacgcga gcgcgtcccg 780
ttcgcgcaga agttccgcgg cgcggacccg caagcgctcg acctcctcga gaagatgctc 840
gtgttcgacc cgcgcaagcg catcgacgcg acgcaggcgc tcgcgcacga gtacctcgca 900
ccgtaccacg acccgacgga cgagcccgtc gcgcccgagg cgtttgactg gagcttcaac 960
gacgcggacc tgccggtgga cacgtggaag gtgatgatgt actcggagat cctcgacttc 1020
caccacatcg aggacgggac cgagtcgggc ccggtcgcgg gcgccgcgcc gcagcagtaa 1080
<210> 2
<211> 360
<212> PRT
<213> Rhodosporidium kratochvilovae
<400> 2
Met Ala Asp Phe Val Lys Leu Ser Ile Phe Gly Thr Val Phe Glu Val Thr Thr Arg Tyr
1 10 20
Val Asp Leu Gln Pro Val Gly Met Gly Ala Phe Gly Leu Val Cys Ser Ala Lys Asp Glu
30 40
Leu Thr Gly Gln MET Val Ala Ile Lys Lys Ile MET Lys Pro Phe Ser Thr Pro Val Leu
50 60
Ala Lys Arg Thr Tyr Arg Glu Leu Lys Leu Leu Lys His Ile Gln His Glu Asn Ile Ile
70 80
Ser Leu Ser Asp Ile Phe Ile Ser Pro Leu Glu Asp Ile Tyr Phe Val Thr Glu Leu Leu
90 100
Gly Thr Asp Leu His Arg Leu Leu Thr Ser Arg Pro Leu Glu Lys Gln Phe Ile Gln Tyr
110 120
Phe Leu Tyr Gln Ile Leu Arg Gly Leu Lys Tyr Val His Ser Ala Gly Val Val His Arg
130 140
Asp Leu Lys Pro Ser Asn Ile Leu Val Asn Glu Asn Cys Asp Leu Lys Ile Cys Asp Phe
150 160
Gly Leu Ala Arg Leu Ala Asp Pro Gln Met Thr Gly Tyr Val Ser Thr Arg Tyr Tyr Arg
170 180
Ala Pro Glu Ile Met Leu Thr Trp Gln Lys Tyr Asp Val Ala Val Asp Ile Trp Ser Ala
190 200
Gly Cys Ile Phe Ala Glu Met Leu Glu Gly Arg Pro Leu Phe Pro Gly Lys Asp His Val
210 220
Asn Gln Phe Ser Ile Ile Thr Glu Leu Leu Gly Thr Pro Pro Asp Glu Val Ile Gln Thr
230 240
Ile Cys Ser Glu Asn Thr Leu Arg Phe Val Gln Ser Leu Pro Lys Arg Glu Arg Val Pro
250 260
Phe Ala Gln Lys Phe Arg Gly Ala Asp Pro Gln Ala Leu Asp Leu Leu Glu Lys MET Leu
270 280
Val Phe Asp Pro Arg Lys Arg Ile Asp Ala Thr Gln Ala Leu Ala His Glu Tyr Leu Ala
290 300
Pro Tyr His Asp Pro Thr Asp Glu Pro Val Ala Pro Glu Ala Phe Asp Trp Ser Phe Asn
310 320
Asp Ala Asp Leu Pro Val Asp Thr Trp Lys Val MET MET Tyr Ser Glu Ile Leu Asp Phe
330 340
His His Ile Glu Asp Gly Thr Glu Ser Gly Pro Val Ala Gly Ala Ala Pro Gln Gln ***
350
<210> 3
<211> 27
<212> DNA
<213> 人工序列
<400> 3
cgcaagctta tggccgactt cgtgaag 27
<210> 4
<211> 26
<212> DNA
<213> 人工序列
<400> 4
taatggatcc ttactgctgc ggcgcg 26
Claims (2)
1.一种高渗透压甘油蛋白激酶基因RkHog1，其核苷酸序列如SEQ ID NO：1所示，该基因编码的氨基酸序列如SEQ ID NO：2所示。
2.一种含有权利要求1所述高渗透压甘油蛋白激酶基因RkHog1的重组表达载体。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710207454.XA CN106916837B (zh) | 2017-03-31 | 2017-03-31 | 高渗透压甘油蛋白激酶基因RkHog1及其重组表达载体 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710207454.XA CN106916837B (zh) | 2017-03-31 | 2017-03-31 | 高渗透压甘油蛋白激酶基因RkHog1及其重组表达载体 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106916837A true CN106916837A (zh) | 2017-07-04 |
| CN106916837B CN106916837B (zh) | 2020-05-15 |
Family
ID=59566985
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710207454.XA Active CN106916837B (zh) | 2017-03-31 | 2017-03-31 | 高渗透压甘油蛋白激酶基因RkHog1及其重组表达载体 |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106916837B (zh) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107267529A (zh) * | 2017-07-20 | 2017-10-20 | 昆明理工大学 | 一种锌指蛋白转录因子基因RkMSN4及其应用 |
| CN108624600A (zh) * | 2018-05-22 | 2018-10-09 | 昆明理工大学 | 锌指转录因子基因RkMsn4的用途 |
| CN111454854A (zh) * | 2020-05-02 | 2020-07-28 | 昆明理工大学 | 一株产虾青素的红冬孢酵母基因工程菌株 |
| CN113430215A (zh) * | 2021-06-03 | 2021-09-24 | 昆明理工大学 | 乙酰CoA合成酶基因RKACS1及其应用 |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5545556A (en) * | 1991-05-23 | 1996-08-13 | William Marsh Rice University | Microorganisms and methods for their use |
| CN103131718A (zh) * | 2012-12-28 | 2013-06-05 | 江南大学 | 来源产甘油假丝酵母新型耐高渗功能基因CgHog1的克隆及其应用 |
| US20150307872A1 (en) * | 2014-04-11 | 2015-10-29 | Wisconsin Alumni Research Foundation | Recombinant yeast having enhanced xylose fermentation capabilities and methods of use |
-
2017
- 2017-03-31 CN CN201710207454.XA patent/CN106916837B/zh active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5545556A (en) * | 1991-05-23 | 1996-08-13 | William Marsh Rice University | Microorganisms and methods for their use |
| CN103131718A (zh) * | 2012-12-28 | 2013-06-05 | 江南大学 | 来源产甘油假丝酵母新型耐高渗功能基因CgHog1的克隆及其应用 |
| US20150307872A1 (en) * | 2014-04-11 | 2015-10-29 | Wisconsin Alumni Research Foundation | Recombinant yeast having enhanced xylose fermentation capabilities and methods of use |
Non-Patent Citations (6)
| Title |
|---|
| GENBANK: "登录号：XM_018412906.1", 《GENBANK》 * |
| GENPEPT: "登录号：XP_018268102.1", 《GENPEPT》 * |
| 冯飞等: "细极链格孢菌Hog1 MAPK(酵母)同源基因AtHOG1的克隆与功能分析", 《华北农学报》 * |
| 岳华等: "转酿酒酵母HOG1基因拟南芥植株的获得与检测", 《西北农业学报》 * |
| 张永军等: "球孢白僵菌Hog1 MAPK同源基因BbHog1的克隆及特征分析", 《菌物学报》 * |
| 王晨莹等: "产甘油假丝酵母HOG1 MAPK同源基因CgHOG1的克隆及特征分析", 《微生物学报》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107267529A (zh) * | 2017-07-20 | 2017-10-20 | 昆明理工大学 | 一种锌指蛋白转录因子基因RkMSN4及其应用 |
| CN108624600A (zh) * | 2018-05-22 | 2018-10-09 | 昆明理工大学 | 锌指转录因子基因RkMsn4的用途 |
| CN111454854A (zh) * | 2020-05-02 | 2020-07-28 | 昆明理工大学 | 一株产虾青素的红冬孢酵母基因工程菌株 |
| CN111454854B (zh) * | 2020-05-02 | 2022-05-06 | 昆明理工大学 | 一株产虾青素的红冬孢酵母基因工程菌株 |
| CN113430215A (zh) * | 2021-06-03 | 2021-09-24 | 昆明理工大学 | 乙酰CoA合成酶基因RKACS1及其应用 |
| CN113430215B (zh) * | 2021-06-03 | 2023-04-18 | 昆明理工大学 | 乙酰CoA合成酶基因RKACS1及其应用 |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106916837B (zh) | 2020-05-15 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105358694B (zh) | 来自巴斯德毕赤酵母的酵母启动子 | |
| CN106916837A (zh) | 高渗透压甘油蛋白激酶基因RkHog1及其重组表达载体 | |
| Walker | Yeasts | |
| Schaechter | Eukaryotic microbes | |
| CN110117602A (zh) | 灰树花udp-葡萄糖焦磷酸化酶及其应用 | |
| CN106834336A (zh) | 一种哈茨木霉酸性蛋白酶p6281的异源表达及纯化方法 | |
| CN107904250A (zh) | 一种黄曲霉致病基因rgfC及其应用 | |
| CN109576244A (zh) | 一种新型脂肪酶及其制备与应用 | |
| CN104630258A (zh) | 一种酿酒酵母基因表达系统及其构建与应用 | |
| CN108034667A (zh) | 一种红色红曲霉α-淀粉酶基因、其制备方法及应用 | |
| CN105602934B (zh) | 酿酒酵母染色体的转移方法 | |
| CN103814134A (zh) | 表达agt1的重组酵母 | |
| CN103773746B (zh) | 一种脂肪酶及其突变体 | |
| CN105441334B (zh) | 产灰树花多糖的菌株及其应用 | |
| CN108558992A (zh) | 调控金针菇子实体发育的转录因子pdd1及其编码基因与应用 | |
| CN112390864A (zh) | Mad1蛋白在调控真菌产孢与萌发及植物亚麻酸代谢路径中的应用 | |
| CN109593769A (zh) | 菰黑粉菌冬孢子形成相关基因Itd1及其应用 | |
| CN107488591A (zh) | 一种高酶活、高耐盐的酱油菌株的选育方法及其应用方法 | |
| CN101165172B (zh) | 一种重组甲基营养杆菌及其应用 | |
| JP4903129B2 (ja) | 耐熱性酵母菌株 | |
| CN102533574A (zh) | 一种低产尿素黄酒酵母工程菌及其构建方法 | |
| CN111849790B (zh) | 重组顶头孢霉工程菌及其构建方法和应用 | |
| CN109251941A (zh) | 一种高产琥珀酸的大肠杆菌及其应用 | |
| CN111411122B (zh) | 稻瘟病菌基因MoHXT2在调控植物糖转运功能中的应用 | |
| Maheshwari | Fungal biology in the 21st century |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |