CN106754683A - 一种人脐带/脂肪间充质干细胞的无分化扩增抗衰老培养基 - Google Patents
一种人脐带/脂肪间充质干细胞的无分化扩增抗衰老培养基 Download PDFInfo
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Abstract
Description
培养基-1 | 培养基-2 | 培养基-3 | 培养基-4 | |
第1天细胞数 | 40万 | 40万 | 40万 | 40万 |
第14天细胞数 | 1.8×108 | 1.6×108 | 2.5×108 | 1.2×108 |
细胞最大um | 18.7±2.6 | 18.9±2.5 | 17.8±1.9 | 19.7±2.9 |
细胞存活率 | 95.5% | 93.9% | 98.7% | 90.1% |
阳性标志物 | 原代 | 第4代 | 第8代 | 第12代 |
CD29 | 99.3 | 99.6 | 99.5 | 99.3 |
CD44 | 99.1 | 99.4 | 99.1 | 99.2 |
CD73 | 98.7 | 99.1 | 99.1 | 98.3 |
CD90 | 99.0 | 99.2 | 98.6 | 98.7 |
HLA-ABC | 99.5 | 99.3 | 99.2 | 99.0 |
CD166 | 99.2 | 99.7 | 99.0 | 99.2 |
PDGFR | 96.4 | 96.6 | 96.8 | 97.1 |
阴性标志物 | 原代 | 第4代 | 第8代 | 第12代 |
CD33 | 0.3 | 0.5 | 0.6 | 0.4 |
CD34 | 1.7 | 0.6 | 0.8 | 0.7 |
CD38 | 0.7 | 0.4 | 0.6 | 0.5 |
CD31 | 1.2 | 0.7 | 0.8 | 0.6 |
CD41 | 0.5 | 0.8 | 0.4 | 0.5 |
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