CN106730568A - A kind of preparation method of biological pesticide residue degrading agent - Google Patents
A kind of preparation method of biological pesticide residue degrading agent Download PDFInfo
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- CN106730568A CN106730568A CN201611030298.6A CN201611030298A CN106730568A CN 106730568 A CN106730568 A CN 106730568A CN 201611030298 A CN201611030298 A CN 201611030298A CN 106730568 A CN106730568 A CN 106730568A
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- A—HUMAN NECESSITIES
- A62—LIFE-SAVING; FIRE-FIGHTING
- A62D—CHEMICAL MEANS FOR EXTINGUISHING FIRES OR FOR COMBATING OR PROTECTING AGAINST HARMFUL CHEMICAL AGENTS; CHEMICAL MATERIALS FOR USE IN BREATHING APPARATUS
- A62D3/00—Processes for making harmful chemical substances harmless or less harmful, by effecting a chemical change in the substances
- A62D3/02—Processes for making harmful chemical substances harmless or less harmful, by effecting a chemical change in the substances by biological methods, i.e. processes using enzymes or microorganisms
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
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- A—HUMAN NECESSITIES
- A62—LIFE-SAVING; FIRE-FIGHTING
- A62D—CHEMICAL MEANS FOR EXTINGUISHING FIRES OR FOR COMBATING OR PROTECTING AGAINST HARMFUL CHEMICAL AGENTS; CHEMICAL MATERIALS FOR USE IN BREATHING APPARATUS
- A62D2101/00—Harmful chemical substances made harmless, or less harmful, by effecting chemical change
- A62D2101/04—Pesticides, e.g. insecticides, herbicides, fungicides or nematocides
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Abstract
The present invention relates to a kind of preparation method of biological pesticide residue degrading agent, belong to degradation agent preparing technical field.The present invention is first with homemade compost culture Triticum tauschii, and sprayed insecticide in incubation and use x-ray bombardment, collect the section section Wheat Straw on compost surface, squeezed the juice after it is mixed with water, collect squeezeding juice and residue of squeezing the juice, squeezeding juice is poured into and is fermented in the compost containing Triticum tauschii root, and by fermentate and glucose solution hybrid filtering, collect upper liquid, to squeeze the juice again after residue is carbonized and be soaked in glucose solution, with the material mixing such as upper liquid, sodium alginate after taking-up.Biological pesticide residue degrading agent prepared by the present invention is suitable for the crops such as wheat, paddy rice, soybean, corn, Chinese cabbage and leek, it is effective to agricultural chemicals such as Fenpropathrin, dimehypo, carbofuran, Furadan, dicofol, triazolone and acephatemets, and it is fast to its degradation speed, can obtain within 9~10 days degradable.
Description
Technical field
The present invention relates to a kind of preparation method of biological pesticide residue degrading agent, belong to degradation agent preparing technical field.
Background technology
After some Organochlorine Pesticide Residues contaminated soils, decades will be remained in soil, and residues of pesticides are once dirty
Dye underground water is difficult to be degraded, and residues of pesticides can form a kind of agricultural production Pollution Chain, and animal is once had residual containing agricultural chemicals
The food that stays, water source will in vivo be enriched with more than 40 times;The mankind have eaten the food such as pollution meat and grain, vegetables containing residues of pesticides
Product, residues of pesticides will be accumulated more than 70 years in human body, endanger of future generation even with women pregnancy and lactation.Residues of pesticides are entered
Entering human body approach has two kinds:One be residues of pesticides on crop, it is directly contaminated;Two are made by the enrichment of food chain
With contaminated food indirectly.When toxic pesticides are applied on crops, fruit and vegetables, the agricultural chemicals in crop surface is remained in
Due to fat-soluble strong, it is easy to the wax coat of people's epidermis is oozed, so that being difficult completely to wash it when using.If received with these
The grain of pollution, vegetables make feed, then the agricultural chemicals for remaining causes pollution in will being transferred to meat, newborn class and egg product, finally with
Food enters human body.
At present, degraded for the biodegrading process Main physical of residues of pesticides, chemical degradation and biodegradation, wherein physics side
Method such as ultrasonic technology, ionising radiation, absorption, peeling, washing etc., but because physical method effective object is only limitted to crop surface
Residues of pesticides and technical equipment requirement it is higher so being difficult to promote on a large scale.Dropped using the more photochemistry that has in chemical degradation
Solution, oxidation Decomposition, hydrolysis etc., common chemicals have certain degradation effect to the residues of pesticides of inside plants, but it is certainly
Body and product are likely to cause secondary pollution again;Because existing every technology all has many drawbacks, far from meeting China
Green agricultural product mass produce and environmental protection requirement, so in the market be badly in need of one kind can spread use, act on
Scope wide spectrum, itself environmentally friendly, safe and efficient chemical residual degradation technology and corresponding chemical residual degradation product.Biology drop
Solution is the main method of current degradating agent for residual agricultural chemicals, including using microorganism, digestive enzyme, engineering bacteria etc..Degrading pesticide is residual
The microbe species for staying mainly have bacterium, fungi, actinomyces, algae etc., by secretase through peroxidating, reduction, hydrolysis, ring crack
Solution, condensation, dehalogenation, de- the approach such as complete, methylate and complete enzymatic degradation.Due to most of agricultural chemicals with halogen, amino, nitro and
Other various substituents etc., the type and quantity of these substitution bases often influence microorganism to the degradability of agricultural chemicals;Additionally,
The pesticide degradation bacteria degradation speed that obtains directly is screened from environment slow, or even can not long term survival, Er Qie in this context
Dominant bacteria can not be turned into contaminated environment.
The content of the invention
The technical problems to be solved by the invention:Directly screened from environment for traditional biological pesticide residue degrading agent
The pesticide degradation bacteria degradation speed of acquisition is slow, in addition can not long term survival in this context, influence the problem of degradation effect, this hair
It is bright and to be sprayed insecticide in incubation and use x-ray bombardment first with homemade compost culture Triticum tauschii, treat culture knot
Shu Hou, collects the section section Wheat Straw on compost surface, is squeezed the juice after it is mixed with water, collects squeezeding juice and residue of squeezing the juice, and will squeeze
Juice is poured into and fermented in the compost containing Triticum tauschii root, and fermentate and glucose solution are carried out into hybrid filtering,
Upper liquid is collected, then is soaked in glucose solution after the residue that will squeeze the juice is carbonized, with upper liquid, sodium alginate etc. after taking-up
Material mixing., using Pond Silt as bacterium source, used as main nutrient matrix, manganese nitrate is used as induction mutation of bacterium for peat for the present invention
Agent, mixes with other nutritional ingredients, the strong Triticum tauschii of culture vitality, in incubation, applies deal agricultural chemicals high, is aided with X
Ray, Screening, Mutation is carried out to the microorganism in compost, while increasing Triticum tauschii resistance to medicine characteristic and mutation, obtains decomposing pesticide
Strain and drug-fast Triticum tauschii, reuse the enzyme that the decomposable asymmetric choice net agricultural chemicals in Triticum tauschii is extracted in fermentation, and having in compost
Beneficial bacteria kind, by carrier loaded, is wrapped up carrier using the PGA of biological degradability high, so as to obtain high de-agglomeration effect
The biological pesticide residue degrading agent of rate, high stability, biological pesticide residue degrading agent degradation speed prepared by the present invention is fast, can be
Exist for a long time in required degraded environment, with good degradation effect.
In order to solve the above technical problems, the technical solution adopted by the present invention is:
(1)Count by weight, take Pond Silt, 28~35 parts of peat, 10~15 parts of beans that 60~65 parts of water content are 50%
The dregs of rice, 7~9 parts of titanium dioxide, 4~7 parts of ammonium sulfate, 3~6 parts of urea and 2~4 parts of manganese nitrates, stir, and obtain compost, then
By compost spreading in culture pond, spreading thickness is 28~38cm, then according to every square metre of 7~12g seed, by Triticum tauschii
Seed is seeded in compost, and depth of planting is 5~8cm, and it is 28~34 DEG C to keep the temperature in culture pond, cultivates Triticum tauschii;
(2)After in the Triticum tauschii tiller 6~8 of above-mentioned culture, the Triticum tauschii after tiller is sprayed using agricultural chemicals, every 26
~32h sprays once, and each fountain height is 1.1~1.4 times that agricultural chemicals specifies fountain height to crops, uses frequency within every 30~35 days
Rate is the x-ray bombardment 7~9s of compost of 70~90PHz, culture Triticum tauschii 3~5 months;
(3)After above-mentioned Triticum tauschii culture terminates, the section section Wheat Straw on compost surface is collected, by the stalk of Triticum tauschii and its matter
The deionized water of 2~3 times of amount is squeezed the juice in being put into juice extractor, collects squeezeding juice and residue of squeezing the juice, and residue of squeezing the juice is standby, by squeezeding juice
Pour into the compost containing Triticum tauschii root, after stirring, the compost after stirring is covered with black thin film, then in black
Wheat stalk is covered on film, cladding thickness is 12~16cm, is fermented 5~7 days;
(4)After after above-mentioned fermentation ends, black thin film and wheat stalk are removed, be to mass fraction is added in fermenting mixture
5% glucose solution, stirs, and when fermenting mixture water content is 65~70%, stops adding glucose solution, quiet
Put 1~2 day, fermenting mixture is filtered using gauze, collect filtered fluid, then filtered fluid stands 20 at room temperature~
30min, collects upper liquid;
(5)By step(3)Standby residue of squeezing the juice is put into carbide furnace, 400~500 DEG C of design temperature, and be carbonized 2~3h, collects
Carbide, then carbide is put into pulverizer is crushed, 150 mesh sieves are crossed, sieving particle is collected, carrier is obtained, by weight
Number meter, takes 70~80 parts of above-mentioned upper liquids, 20~25 parts of carriers, 16~19 parts of sodium alginates, 12~14 parts of PGAs and 20
~24 parts of hexafluoroisopropanols, carrier are soaked in the glucose solution that mass fraction is 7~8% and soak 2~3h, are taken out and are carried
Body;
(6)By the above-mentioned upper liquid in the carrier after above-mentioned taking-up and above-mentioned parts by weight, sodium alginate, PGA and hexafluoro
Isopropanol is put into mixer, and 30~50min is stirred with 130r/min, and stirrer for mixing thing then is put into Rotary Evaporators
In, to take out and be depressed into 70~80kPa, design temperature is 30~35 DEG C, and hexafluoroisopropanol is reclaimed in evaporation, then to remaining in Rotary Evaporators
Thing is filtered, and is collected during filter residue is put into air-dry machine and is air-dried, and collects air dry matter, you can obtain biological pesticide residue degrading agent.
Application process of the invention:Biological pesticide residue degrading agent prepared by the present invention is applied to wheat, paddy rice, big
In the crops such as beans, corn, Chinese cabbage and leek, first in mass ratio 1:100~1:150, the present invention is prepared into biological pesticide residual
After staying degradation agent to be stirred with water and be well mixed, obtain diluting degradation agent, then after pesticide spraying 3~5 days, will dilution degraded
Agent is sprayed in farmland, and the amount of sprinkling is 40~45kg/hm2, sprinkling completion.After testing, 2~3 days degradation of pesticide rates reach
To more than 65%, degradation of pesticide rate reaches more than 95% within 5~6 days, and agricultural chemicals obtains degradable within 9~10 days, noresidue, 14~18
Agent of biological pesticide residue degrading is degradable after it.
Compared with other method, Advantageous Effects are the present invention:
(1)Biological pesticide residue degrading agent prepared by the present invention is applied to the agricultures such as wheat, paddy rice, soybean, corn, Chinese cabbage and leek
In crop, the agricultural chemicals remained in crops is greatly reduced;
(2)Biological pesticide residue degrading agent prepared by the present invention is to Fenpropathrin, dimehypo, carbofuran, Furadan, three chlorbensides
The agricultural chemicals such as alcohol, triazolone and acephatemet effectively, and to its degradation speed soon, can be obtained degradable for 9~10 days;
(3)Biological pesticide residue degrading agent preparation process prepared by the present invention is simple, and required raw material obtain simple, low cost
It is honest and clean.
Specific embodiment
Count by weight first, take Pond Silt, 28~35 parts of peat, 10~15 that 60~65 parts of water content are 50%
Part dregs of beans, 7~9 parts of titanium dioxide, 4~7 parts of ammonium sulfate, 3~6 parts of urea and 2~4 parts of manganese nitrates, stir, and must cultivate
Soil, then by compost spreading in culture pond, spreading thickness is 28~38cm, then according to every square metre of 7~12g seed, will
Section section Wheat Seeds are seeded in compost, and depth of planting is 5~8cm, and it is 28~34 DEG C to keep the temperature in culture pond, culture section
Section wheat;After in the Triticum tauschii tiller 6~8 of above-mentioned culture, the Triticum tauschii after tiller is sprayed using agricultural chemicals, every 26~
32h sprays once, and each fountain height is 1.1~1.4 times that agricultural chemicals specifies fountain height to crops, every 30~35 days frequency of use
It is the x-ray bombardment 7~9s of compost of 70~90PHz, culture Triticum tauschii 3~5 months;Terminate in above-mentioned Triticum tauschii culture again
Afterwards, the section section Wheat Straw on compost surface is collected, 2~3 times of deionized water of the stalk of Triticum tauschii and its quality is put into and is squeezed the juice
Squeezed the juice in machine, collect squeezeding juice and residue of squeezing the juice, residue of squeezing the juice is standby, and squeezeding juice is poured into the compost containing Triticum tauschii root
In, after stirring, the compost after stirring is covered with black thin film, then wheat stalk is covered on black thin film, covering is thick
It is 12~16cm to spend, and is fermented 5~7 days;After after above-mentioned fermentation ends, black thin film and wheat stalk are removed, to fermenting mixture
It is middle to add the glucose solution that mass fraction is 5%, stir, when fermenting mixture water content is 65~70%, stop
Glucose solution is added, 1~2 day is stood, fermenting mixture is filtered using gauze, collect filtered fluid, then by filtered fluid
20~30min is stood at room temperature, collects upper liquid;Then standby residue of squeezing the juice is put into carbide furnace, design temperature 400
~500 DEG C, be carbonized 2~3h, collects carbide, then carbide is put into pulverizer is crushed, and crosses 150 mesh sieves, collected
Sieve particle, obtains carrier, counts by weight, takes 70~80 parts of above-mentioned upper liquids, 20~25 parts of carriers, 16~19 parts of alginic acids
Sodium, 12~14 parts of PGAs and 20~24 parts of hexafluoroisopropanols, the glucose that mass fraction is 7~8% is soaked in by carrier
2~3h is soaked in solution, carrier is taken out;Finally by the above-mentioned upper liquid in the carrier after above-mentioned taking-up and above-mentioned parts by weight,
Sodium alginate, PGA and hexafluoroisopropanol are put into mixer, and 30~50min is stirred with 130r/min, then will stirring
Mixture is put into Rotary Evaporators in machine, is taken out and is depressed into 70~80kPa, and design temperature is 30~35 DEG C, and it is different that hexafluoro is reclaimed in evaporation
Propyl alcohol, then residue in Rotary Evaporators is filtered, collect during filter residue is put into air-dry machine and air-dry, collect air dry matter, you can
Obtain biological pesticide residue degrading agent.
Example 1
Count by weight first, take Pond Silt, 35 parts of peat, 15 parts of dregs of beans, 9 parts of titanium dioxides that 65 parts of water content are 50%
Titanium, 7 parts of ammonium sulfate, 6 parts of urea and 4 parts of manganese nitrates, stir, and obtain compost, then compost spreading is spread in culture pond
Thickness is spread for 38cm, then according to every square metre of 12g seed, section section Wheat Seeds is seeded in compost, depth of planting is
8cm, it is 34 DEG C to keep the temperature in culture pond, cultivates Triticum tauschii;After in the Triticum tauschii tiller 8 of above-mentioned culture, agriculture is used
Medicine sprays to the Triticum tauschii after tiller, and per 32h, once, each fountain height is agricultural chemicals to crops regulation fountain height for sprinkling
1.4 times, every 35 days frequency of use are the x-ray bombardment compost 9s of 90PHz, culture Triticum tauschii 5 months;Again in above-mentioned Triticum tauschii
After culture terminates, the section section Wheat Straw on compost surface is collected, 3 times of deionized water of the stalk of Triticum tauschii and its quality is put into
Squeezed the juice in juice extractor, collect squeezeding juice and residue of squeezing the juice, residue of squeezing the juice is standby, and squeezeding juice is poured into the training containing Triticum tauschii root
Support in soil, after stirring, the compost after stirring is covered with black thin film, then wheat stalk is covered on black thin film, cover
Lid thickness is 16cm, is fermented 7 days;After after above-mentioned fermentation ends, black thin film and wheat stalk are removed, added in fermenting mixture
Enter the glucose solution that mass fraction is 5%, stir, when fermenting mixture water content is 70%, stop adding grape
Sugar juice, stands 2 days, and fermenting mixture is filtered using gauze, collects filtered fluid, then filtered fluid is stood at room temperature
30min, collects upper liquid;Then standby residue of squeezing the juice is put into carbide furnace, 500 DEG C of design temperature, be carbonized 3h, collects carbon
Compound, then carbide is put into pulverizer is crushed, 150 mesh sieves are crossed, sieving particle is collected, carrier is obtained, by weight
Meter, takes 80 parts of above-mentioned upper liquids, 25 parts of carriers, 19 parts of sodium alginates, 14 parts of PGAs and 24 parts of hexafluoroisopropanols, by carrier
It is soaked in the glucose solution that mass fraction is 8% and soaks 3h, takes out carrier;Finally by the carrier after above-mentioned taking-up with it is above-mentioned
Above-mentioned upper liquid, sodium alginate, PGA and hexafluoroisopropanol in parts by weight are put into mixer, are stirred with 130r/min
50min is mixed, then stirrer for mixing thing is put into Rotary Evaporators, taken out and be depressed into 80kPa, design temperature is 35 DEG C, evaporation
Hexafluoroisopropanol is reclaimed, then residue in Rotary Evaporators is filtered, collected during filter residue is put into air-dry machine and air-dry, collect wind
Dry, you can obtain biological pesticide residue degrading agent.
Biological pesticide residue degrading agent prepared by the present invention is applied in wheat, first in mass ratio 1:100, this is sent out
It is bright to prepare after the agent of biological pesticide residue degrading is stirred with water and is well mixed, obtain diluting degradation agent, then in agricultural chemicals first cyanogen chrysanthemum
After ester sprays 5 days, dilution degradation agent is sprayed in farmland, the amount of sprinkling is 45kg/hm2, sprinkling completion.After testing, 3
Its Fenpropathrin degradation rate reaches 67%, and Fenpropathrin degradation rate reaches 96% within 5 days, and Fenpropathrin obtains degradable within 10 days,
Noresidue, the agent of biological pesticide residue degrading is degradable after 14 days.
Example 2
Count by weight first, take Pond Silt, 28 parts of peat, 10 parts of dregs of beans, 7 parts of titanium dioxides that 60 parts of water content are 50%
Titanium, 4 parts of ammonium sulfate, 3 parts of urea and 2 parts of manganese nitrates, stir, and obtain compost, then compost spreading is spread in culture pond
Thickness is spread for 28cm, then according to every square metre of 7g seed, section section Wheat Seeds is seeded in compost, depth of planting is 5cm,
It is 28 DEG C to keep the temperature in culture pond, cultivates Triticum tauschii;After in the Triticum tauschii tiller 6 of above-mentioned culture, agricultural chemicals pair is used
Triticum tauschii after tiller is sprayed, and per 26h, once, each fountain height is agricultural chemicals to the 1.1 of crops regulation fountain height for sprinkling
Times, every 30 days frequency of use are the x-ray bombardment compost 7s of 70PHz, culture Triticum tauschii 3 months;Trained in above-mentioned Triticum tauschii again
Support after terminating, collect the section section Wheat Straw on compost surface, the deionized water of 2 times of the stalk of Triticum tauschii and its quality is put into squeezing
Squeezed the juice in juice machine, collect squeezeding juice and residue of squeezing the juice, residue of squeezing the juice is standby, and squeezeding juice is poured into the culture containing Triticum tauschii root
In soil, after stirring, the compost after stirring is covered with black thin film, then wheat stalk is covered on black thin film, covered
Thickness is 12cm, is fermented 5 days;After after above-mentioned fermentation ends, black thin film and wheat stalk are removed, added in fermenting mixture
Mass fraction is 5% glucose solution, is stirred, and when fermenting mixture water content is 65%, stops adding glucose
Solution, stands 1 day, and fermenting mixture is filtered using gauze, collects filtered fluid, then filtered fluid is stood at room temperature
20min, collects upper liquid;Then standby residue of squeezing the juice is put into carbide furnace, 400 DEG C of design temperature, be carbonized 2h, collects carbon
Compound, then carbide is put into pulverizer is crushed, 150 mesh sieves are crossed, sieving particle is collected, carrier is obtained, by weight
Meter, takes 70 parts of above-mentioned upper liquids, 20 parts of carriers, 16 parts of sodium alginates, 12 parts of PGAs and 20 parts of hexafluoroisopropanols, by carrier
It is soaked in the glucose solution that mass fraction is 7% and soaks 2h, takes out carrier;Finally by the carrier after above-mentioned taking-up with it is above-mentioned
Above-mentioned upper liquid, sodium alginate, PGA and hexafluoroisopropanol in parts by weight are put into mixer, are stirred with 130r/min
30min is mixed, then stirrer for mixing thing is put into Rotary Evaporators, taken out and be depressed into 70kPa, design temperature is 30 DEG C, evaporation
Hexafluoroisopropanol is reclaimed, then residue in Rotary Evaporators is filtered, collected during filter residue is put into air-dry machine and air-dry, collect wind
Dry, you can obtain biological pesticide residue degrading agent.
Biological pesticide residue degrading agent prepared by the present invention is applied in leek, first in mass ratio 1:100~1:
150, the present invention is prepared after the agent of biological pesticide residue degrading is stirred with water and is well mixed, obtain diluting degradation agent, then
After the double sprinklings of agricultural insecticide 3 days, dilution degradation agent is sprayed in farmland, the amount of sprinkling is 40kg/hm2, sprinkling completion.
After testing, 2 days dimehypo degradation rates reach 66%, and dimehypo degradation rate reaches 98% within 5 days, and dimehypo obtains degradable within 9 days,
Noresidue, the agent of biological pesticide residue degrading is degradable after 18 days.
Example 3
Count by weight first, take Pond Silt, 30 parts of peat, 12 parts of dregs of beans, 8 parts of titanium dioxides that 62 parts of water content are 50%
Titanium, 6 parts of ammonium sulfate, 5 parts of urea and 3 parts of manganese nitrates, stir, and obtain compost, then compost spreading is spread in culture pond
Thickness is spread for 30cm, then according to every square metre of 10g seed, section section Wheat Seeds is seeded in compost, depth of planting is
7cm, it is 30 DEG C to keep the temperature in culture pond, cultivates Triticum tauschii;After in the Triticum tauschii tiller 7 of above-mentioned culture, agriculture is used
Medicine sprays to the Triticum tauschii after tiller, and per 30h, once, each fountain height is agricultural chemicals to crops regulation fountain height for sprinkling
1.3 times, every 32 days frequency of use are the x-ray bombardment compost 8s of 80PHz, culture Triticum tauschii 4 months;Again in above-mentioned Triticum tauschii
After culture terminates, the section section Wheat Straw on compost surface is collected, 3 times of deionized water of the stalk of Triticum tauschii and its quality is put into
Squeezed the juice in juice extractor, collect squeezeding juice and residue of squeezing the juice, residue of squeezing the juice is standby, and squeezeding juice is poured into the training containing Triticum tauschii root
Support in soil, after stirring, the compost after stirring is covered with black thin film, then wheat stalk is covered on black thin film, cover
Lid thickness is 15cm, is fermented 6 days;After after above-mentioned fermentation ends, black thin film and wheat stalk are removed, added in fermenting mixture
Enter the glucose solution that mass fraction is 5%, stir, when fermenting mixture water content is 66%, stop adding grape
Sugar juice, stands 1 day, and fermenting mixture is filtered using gauze, collects filtered fluid, then filtered fluid is stood at room temperature
25min, collects upper liquid;Then standby residue of squeezing the juice is put into carbide furnace, 450 DEG C of design temperature, be carbonized 3h, collects carbon
Compound, then carbide is put into pulverizer is crushed, 150 mesh sieves are crossed, sieving particle is collected, carrier is obtained, by weight
Meter, takes 75 parts of above-mentioned upper liquids, 22 parts of carriers, 17 parts of sodium alginates, 13 parts of PGAs and 22 parts of hexafluoroisopropanols, by carrier
It is soaked in the glucose solution that mass fraction is 7% and soaks 2h, takes out carrier;Finally by the carrier after above-mentioned taking-up with it is above-mentioned
Above-mentioned upper liquid, sodium alginate, PGA and hexafluoroisopropanol in parts by weight are put into mixer, are stirred with 130r/min
40min is mixed, then stirrer for mixing thing is put into Rotary Evaporators, taken out and be depressed into 75kPa, design temperature is 32 DEG C, evaporation
Hexafluoroisopropanol is reclaimed, then residue in Rotary Evaporators is filtered, collected during filter residue is put into air-dry machine and air-dry, collect wind
Dry, you can obtain biological pesticide residue degrading agent.
Biological pesticide residue degrading agent prepared by the present invention is applied in corn, first in mass ratio 1:120, this is sent out
It is bright to prepare after the agent of biological pesticide residue degrading is stirred with water and is well mixed, obtain diluting degradation agent, then in agricultural chemicals acephatemet
After sprinkling 4 days, dilution degradation agent is sprayed in farmland, the amount of sprinkling is 42kg/hm2, sprinkling completion.After testing, 2 days
Methamidophos degradation rate reaches 68%, and methamidophos degradation rate reaches 98% within 5 days, and acephatemet obtains degradable within 9 days, noresidue, 16
Agent of biological pesticide residue degrading is degradable after it.
Claims (1)
1. a kind of preparation method of biological pesticide residue degrading agent, it is characterised in that specific preparation process is:
(1)Count by weight, take Pond Silt, 28~35 parts of peat, 10~15 parts of beans that 60~65 parts of water content are 50%
The dregs of rice, 7~9 parts of titanium dioxide, 4~7 parts of ammonium sulfate, 3~6 parts of urea and 2~4 parts of manganese nitrates, stir, and obtain compost, then
By compost spreading in culture pond, spreading thickness is 28~38cm, then according to every square metre of 7~12g seed, by Triticum tauschii
Seed is seeded in compost, and depth of planting is 5~8cm, and it is 28~34 DEG C to keep the temperature in culture pond, cultivates Triticum tauschii;
(2)After in the Triticum tauschii tiller 6~8 of above-mentioned culture, the Triticum tauschii after tiller is sprayed using agricultural chemicals, every 26
~32h sprays once, and each fountain height is 1.1~1.4 times that agricultural chemicals specifies fountain height to crops, uses frequency within every 30~35 days
Rate is the x-ray bombardment 7~9s of compost of 70~90PHz, culture Triticum tauschii 3~5 months;
(3)After above-mentioned Triticum tauschii culture terminates, the section section Wheat Straw on compost surface is collected, by the stalk of Triticum tauschii and its matter
The deionized water of 2~3 times of amount is squeezed the juice in being put into juice extractor, collects squeezeding juice and residue of squeezing the juice, and residue of squeezing the juice is standby, by squeezeding juice
Pour into the compost containing Triticum tauschii root, after stirring, the compost after stirring is covered with black thin film, then in black
Wheat stalk is covered on film, cladding thickness is 12~16cm, is fermented 5~7 days;
(4)After after above-mentioned fermentation ends, black thin film and wheat stalk are removed, be to mass fraction is added in fermenting mixture
5% glucose solution, stirs, and when fermenting mixture water content is 65~70%, stops adding glucose solution, quiet
Put 1~2 day, fermenting mixture is filtered using gauze, collect filtered fluid, then filtered fluid stands 20 at room temperature~
30min, collects upper liquid;
(5)By step(3)Standby residue of squeezing the juice is put into carbide furnace, 400~500 DEG C of design temperature, and be carbonized 2~3h, collects
Carbide, then carbide is put into pulverizer is crushed, 150 mesh sieves are crossed, sieving particle is collected, carrier is obtained, by weight
Number meter, takes 70~80 parts of above-mentioned upper liquids, 20~25 parts of carriers, 16~19 parts of sodium alginates, 12~14 parts of PGAs and 20
~24 parts of hexafluoroisopropanols, carrier are soaked in the glucose solution that mass fraction is 7~8% and soak 2~3h, are taken out and are carried
Body;
(6)By the above-mentioned upper liquid in the carrier after above-mentioned taking-up and above-mentioned parts by weight, sodium alginate, PGA and hexafluoro
Isopropanol is put into mixer, and 30~50min is stirred with 130r/min, and stirrer for mixing thing then is put into Rotary Evaporators
In, to take out and be depressed into 70~80kPa, design temperature is 30~35 DEG C, and hexafluoroisopropanol is reclaimed in evaporation, then to remaining in Rotary Evaporators
Thing is filtered, and is collected during filter residue is put into air-dry machine and is air-dried, and collects air dry matter, you can obtain biological pesticide residue degrading agent.
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