CN102719372A - Dicofol degrading bacterium and soil restoration application - Google Patents
Dicofol degrading bacterium and soil restoration application Download PDFInfo
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- CN102719372A CN102719372A CN2012101405025A CN201210140502A CN102719372A CN 102719372 A CN102719372 A CN 102719372A CN 2012101405025 A CN2012101405025 A CN 2012101405025A CN 201210140502 A CN201210140502 A CN 201210140502A CN 102719372 A CN102719372 A CN 102719372A
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Abstract
The invention discloses a dicofol degrading bacterium and a soil restoration application. The dicofol degrading bacterium provided by the invention is bacterial strain DCF-7 of pseudomonas putida, the bacterium can completely degrade 200mg/L of dicofol in 7days under an aerobic condition, the bacterial strain DCF-7 is sensitive to gentamycin, neomycin and tetracycline, and presents tolerability to 13 types of heavy metals; the dicofol degrading bacterium has the capability for degrading dicofol with high efficiency under actual soil environment, can substantially shorten the timing for degrading dicofol, and has significance and value of practical application.
Description
Technical field
The invention belongs to environmental pollutant biologic treating technique field, be specifically related to the bacterium and the application in soil pollution is repaired thereof of a high-efficiency degradation kelthane.
Background technology
Agricultural chemicals is being brought into play important role as important agricultural material at aspects such as protection agriculture prodn, raising overall productivity in agriculture, the raising the output of promotion stable food and peasant continue to increase income.China is pesticide producing big country, also is to use big country, and year usage quantity all occupies the first in the world with average consumption.Yet the utilization ratio of agricultural chemicals is less than 20% but, and rest part all gets into soil, water body and atmosphere, has formed serious pollution and harm.Statistic data shows that China receives the farmland area of pesticide residual contamination to be about 1,500 ten thousand hectares, accounts for and can plough 10% of area, and nearly half crop pesticide residue exceed standard, and suffer exit loss because of the residual problem of exceeding standard of farming and exceed 8,000,000,000 dollars every year.
Organochlorine pesticide has characteristics such as high residue and high enrichment, is typical persistence organic pollutant in the environment.In case in the entering environment, can grow Distance Transmission and accumulation, get into varying environment once more, the final pollution that forms the whole ecological system in the strange land.China begins to develop organochlorine pesticide from the 1950's; Mainly comprise products such as phenyl-hexachloride, compound 118, Dieldrin-attapulgite mixture, Niran, kelthane and heptachlor; They once brought into play significant role in industrial and agricultural production; But because this compounds has extremely strong inrichment in food chain, be important environmental classes hormone and persistence organic pollutant, major part has stopped producing and using.So far, this pesticide residue still constantly threatening national health, restricts agriculture benign development.
Kelthane (Dicofol), another name Mitigan, chemical name 2,2,2-three chloro-1, two (4-chloro-phenyl-) ethanol of 1-.Belonging to the broad spectrum miticide, is China's miticide agricultural chemicals commonly used at present, can prevent and treat the mite class on the crops such as cotton, fruit tree and vegetables, large usage quantity; Environment degradable is slow, and residual quantity is high, and dispenser still can detect residual after 1 year; Long-term application causes the harmful mite of some kind to develop immunity to drugs, and harm increases the weight of; In the water surrounding, acute toxicity is quite high, and research shows its LC to oyster and rainbow trout
50Value is respectively 15 μ g/L and 120 μ g/L.In addition, increasing research shows that kelthane can produce the endocrine system of animals such as fish, birds and mouse and disturb, and the mankind are also had stronger female hormone effect in environment.
Biological degradation is the main path that kelthane is removed in the environment, at present through technology such as artificial enrichment culture, has separated obtaining many strains kelthane degradation bacteria.Yet efficient degrading bacterial strain is very limited, and most bacterial strains are degradation rate less thaies 88% on the 7th of 100 mg/L kelthanes to concentration.People's results of study such as Osman show that each strains tested is respectively degradation rates on the 7th of 100 mg/L kelthanes
Azotobacter chroococcum80%,
Klebsilense pneumoneae61%,
Pseudomona cepacia73%,
Bacillus subtilis68%,
P. fluorescences74%,
B. polymyxa70%,
Azospirillium barasilense76%,
Trichoderma viride(viride) 84% draw
T. harzianum(trichoderma harziarum) 87%.Every day, degradation rate was followed successively by: 11.4 mg/L, 8.7 mg/L, 10.4 mg/L, 9.7 mg/L; 10.6 mg/L, 10.0 mg/L, 10.9 mg/L; 12.0 mg/L and 12.4 mg/L (Osman K.A., Ibrahim G.H., Askar A.I.; Aba Alkhail A.R.A. Biodegradation kinetics of dicofol by selected microorganisms. Pesticide Biochemistry and Physiology, 2008,91:180-185).In addition, the antibiotics resistance of above-mentioned degradation bacteria and heavy metal tolerance etc. does not all add to be verified, and exists certain environment to use risk.
In sum, screen more efficiently, responsive and can tolerate various heavy to common antibiotics, and it is extremely urgent under actual edatope, to possess the degradation bacteria strains of kelthane degradation capability.
Summary of the invention
The objective of the invention is problem or deficiency, a kind of efficient kelthane degradation bacteria is provided to above-mentioned existing kelthane microbiological deterioration existence.
Another object of the present invention provides the application of above-mentioned bacterial classification in repairing the kelthane contaminated soil.
Above-mentioned technical problem of the present invention is able to implement through following technical scheme:
One strain kelthane degradation bacteria, this bacterium be pseudomonas putida (
Pseudomonas putida) bacterial strain DCF-7, on April 1st, 2012 in the preservation of Chinese common micro-organisms culture presevation administrative center, be numbered CGMCC No. 5949, this bacterium has the ability of degraded kelthane.
The application of a kind of said kelthane degradation bacteria in receiving the reparation of kelthane contaminated soil.
As preferably, said bacterial strain CGMCC No. 5949 is seeded in the basic inorganic salt liquid substratum that contains kelthane 50-200 mg/L, 120 r/min concussion is cultivated more than 48 h, and described basic inorganic salt liquid substratum composition is (g/L): MgCl
20.2, NH
4NO
31, KH
2PO
42, K
2HPO
47.5 NaCl 1, deionized water 1 L.
As preferably, described liquid culture temperature is 25-30 ℃, and the pH of basic inorganic salt liquid substratum is 5.8-7.0.
As preferably, described liquid culture temperature is 30 ℃, and the pH of basic inorganic salt liquid substratum is 7.0.
As preferably, said bacterial strain CGMCC No. 5949 is seeded to 30 ℃ of constant temperature culture obtained the thalline fermented liquid more than 24 hours in the fermention medium, degrade to receiving the kelthane Contaminated soil to spray the thalline fermented liquid; The composition of fermention medium is (g/L): Semen Maydis powder 10, bean cake powder 8, (NH
4)
2SO
46, steeping water 6, MgSO
47H
2O 1, K
2HPO
43H
2O 2, MnSO
40.05 pH 7.0.
Kelthane degradation bacteria provided by the present invention
Pseudomonas putidaDCF-7 is derived near the soil of Tonglu, Zhejiang insecticide factory sewage draining exit, obtains through artificial enrichment culture, pressure screening and separation and purification.This Pseudomonas pseudomonas putida, its phylogeny analysis on Position is as shown in Figure 1.It is characterized in that: Gram-negative, catalase is positive, oxidase negative; V. the P. test is negative, and indole test is negative, and thalli morphology is a rod-short; That polar flagella, bacterium colony are is faint yellow, circular, the edge is neat, smooth moistening, other physiological and biochemical property is as shown in table 1.The GenBank accession number of bacterial strain 16S rDNA sequence is JQ837896, on April 1st, 2012 in the preservation of Chinese common micro-organisms culture presevation administrative center, be numbered CGMCC No. 5949.
Annotate: "+" expression growth or positive reaction; "-" expression is not grown or negative reaction.
The optimum growing condition of this bacterium is: pH 7.0, and temperature is 30 ℃.With 1/5th LB (Luria-Bertani) or industrial fermentation substratum (Semen Maydis powder 10 g/L, bean cake powder 8 g/L, (NH
4)
2SO
46 g/L, steeping water 6 g/L, MgSO
47H
2O 1 g/L, K
2HPO
43H
2O 2 g/L, MnSO
40.05 g/L, pH 7.0) to cultivate after 24 hours, bacterium liquid cell concentration can reach 6-9 * 10
9CFU/mL (CFU, colony-forming unit).
This bacterium is to qingfengmeisu qiong, Xin Meisu and sensitive tetracycline, and the three is all common antibiotics.Can think: when thalline is released in the physical environment, can be because of not resisting (anti-) property of medicine problem to produce superbacteria or between indigenous bacterium, propagating.
This bacterium is to 13 kinds of test metals ion (Li
+, Ag
+, Cu
+, Cu
2+, Hg
2+, Ba
2+, Pb
2+, Zn
2+, Mn
2+, Ni
2+, Fe
2+, Fe
3+And Co
4+) present tolerance in various degree.In view of China's soil pollution present situation---persistence organic pollutant pollution and heavy metal contamination are also deposited, it is very important that the pesticide residual degradation bacterial strain possesses heavy metal tolerance performance.Can think: under the general soil envrionment conditions, this bacterial strain thalline is used repairing effect and is received heavy metal to suppress to influence less.
(volume is the triangular flask of 250 mL to this bacterial strain, and 80 mL liquid minimal mediums are housed, and kelthane concentration is 200 mg/L, and the initial inoculation cell concentration is 1 * 10 in shaking bottle degraded test
7Individual/mL, shaking speed is 150 r/min, and temperature is 30 ℃), can in 7 days, degrade fully and supply examination kelthane (degradation rate is mg/L every days 28.57).Supplying examination tea garden soil kelthane content is under the 50 mg/kg situation, uses this bacterium 84.6% kelthane is degraded, and significantly promotes the kelthane degradation rate, shortens the duration of degrading, and possesses practical application meaning and value.
In sum, the present invention compares with prior art and has following advantage:
Compared with prior art, of the present invention
Pseudomonas putidaThe DCF-7 bacterial strain can be grown as sole carbon source with kelthane under aerobic condition and breed, simultaneously with its quick degraded.Under the pure culture condition, it can be that the kelthane of 200 mg/L was degraded in 7 days fully with concentration.Compare with existing degradation bacteria strains,
Pseudomonas putidaDCF-7 kelthane degradation rate is higher, and degradation rate is the fastest.With
T. harzianum(trichoderma harziarum) is example, and this bacterium kelthane degradation rate on the 7th is that degradation rate side on the 87%, 14 is 95%, can't realize degrading fully; The kelthane degradation rate was 12.4 mg/L on 7th, was merely 43.4% of the DCF-7 degradation rate same period; In addition, compare with bacterium, obviously not enough below the filamentous fungus degradation bacteria exists: 1) growth velocity is slow.The bacterium industrial fermentation is generally between 2-3 days, and the filamentous fungus fermentation then is no less than 4, how between 6-8 day.With regard to the general industry fermentation, the ferment product cell concentration can reach 1-5 * 10
9CFU/mL, fungus solids fermentation spore count then will hang down an one magnitude; 2) the fermentation equipment versatility is poor.Filamentous fungus fermentation solid fermentation bed or the fermentor tanks of adopting, equipment interoperability is poor more.Fermentation using bacteria also can be used for secondary metabolite production with fermentor tank except that producing the thalline, like microbiotic, pigment and VITAMINs etc., universal performance is good; 3) the microbial inoculum application effect is slow relatively.After the bacterium microbial inoculum sprayed, bacterium directly acted on pesticide residue, and the filamentous fungus spore then need be treated can act on behind sprouting, the formation mycelia.
In addition,
Pseudomonas putidaDCF-7 is responsive to common antibiotics; 13 heavy metal species are presented tolerance; And possess the ability of performance kelthane Degradation under actual edatope condition, this bacterial strain is expected in the coenocorrelation reparation of kelthane contaminated soil, play a significant role.
Description of drawings
Fig. 1 is the phylogeny analysis on Position of bacterial strain DCF-7;
Fig. 2 is the kelthane degradation capability test result of bacterial strain DCF-7.
Fig. 3 is the kelthane degraded situation in the bacterial strain DCF-7 edatope, wherein, and: kelthane content in the deactivation soil sample; ■: spray kelthane content in the DCF-7 thalline deactivation soil sample; △: kelthane content in the fresh soil sample; ▲: spray kelthane content in the DCF-7 thalline fresh soil sample.
Embodiment
Through specific embodiment, technical scheme of the present invention is further specified below.Should be appreciated that enforcement of the present invention is not limited to following embodiment, all will fall into protection domain of the present invention any pro forma accommodation and/or the change that the present invention made.
In the present invention, if not refer in particular to, all part, per-cents are weight unit, and all equipment and raw material etc. all can be buied from market or the industry is commonly used.
Embodiment 1: Pseudomonas putidaThe separation of DCF-7 and kelthane degradation capability
Near the collection in worksite Zhejiang Tonglu insecticide factory sewage draining exit soil with 150 mL triangular flask splendid attires, and is taken back the laboratory rapidly.The sample of fetching is placed kelthane selectivity basis inorganic salt liquid substratum acclimation shaking culture.The substratum composition is (g/L): MgCl
20.2, NH
4NO
31, KH
2PO
42, K
2HPO
47.5 NaCl 1, kelthane 0.15 (being dissolved in acetone in advance), and pH 6.8, deionized water 1 L, 115 ℃ of moist heat sterilizations are subsequent use after 30 minutes.Culture condition is: 25 ℃, and 120 r/min shaking culture, the container liquid amount is 1/3 of a vessel volume.After this, every will shaking at a distance from 5 days a bottle static a moment abandoned supernatant, mends the selected liq substratum and the continuation vibration enrichment culture of the fresh configuration of equivalent subsequently.After six weeks, above-mentioned culture is evenly coated on the solid selectivity basis minimal medium (agar that adds 20 grams in every liter of liquid nutrient medium again).Coated flat board is inverted into incubator, cultivated 72 hours for 28 ℃.With aseptic toothpick select single bacterium colony or with inoculating needle line until occur single bacterium colony again row be forwarded on the solid selectivity flat board of fresh configuration; And verifying the kelthane degradation capability of each bacterial strain one by one through vapor-phase chromatography, the result obtains a strain kelthane degradation bacteria DCF-7.
It is that the initial inoculation cell concentration is 1 * 10 in the selectivity basis minimal medium of sole carbon source that bacterial strain DCF-7 is inserted with 200 mg/L kelthanes
7Individual/mL, 30 ℃, 150 r/min shaking culture.Subsequently, whenever at a distance from 1 day to nutrient solution in the residual condition of kelthane test, the result is as shown in Figure 2.Can find out that therefrom bacterial strain DCF-7 can kelthane be that sole carbon source is grown, and realizes the degraded fully to kelthane simultaneously.
Present embodiment explanation separation obtains
Pseudomonas putidaDCF-7 kelthane capable of using carries out growth and breeding as sole carbon source, and has the ability of efficient degradation kelthane.
Embodiment 2: Pseudomonas putidaThe resistance experiment of DCF-7
Adopt the filter paper method, select tsiklomitsin, qingfengmeisu qiong and Xin Meisu to test, big or small as responsive or drug-fast judgment basis with each microbiotic filter paper (30 μ g/ sheet) at the antibacterial circle diameter of cultivating on the degradation bacteria DCF-7 flat board.Test-results shows that antibacterial circle diameter is followed successively by 24,28 and 32 mm, and (sensitive range is: tsiklomitsin medium sensitivity scope 23-25 mm to be moderate, height and extreme sensitivity response respectively; The extremely sensitive scope 26-30 mm of qingfengmeisu qiong; Xin Meisu is sensitive range＞30 mm extremely).
The present embodiment explanation can be because of anti-(anti-) property of medicine problem produces superbacteria, for its practical application from now on provides safety assurance when thalline is released in the physical environment.
Embodiment 3: Pseudomonas putidaHeavy metal minimum inhibition concentration (MIC) test of DCF-7
The MIC test design is carried out (Filali B.K., Taoufik J., Zeroual Y. with reference to people's such as Filali research; Dzairi F.Z., Talbi M., Blaghen M. Waste water bacterial isolates resistant to heavy metals and antibiotics. Current Microbiology; 2000,41:151-156), picking etc. are big, the single bacterium colony of the DCF-7 of activated processing is in culture test tube; 30 ℃, 150 r/min shaking culture 48 hours.Judge the MIC value according to the thalline growing way, establish 3 repetitions for every group, test the tolerance situation of this bacterium respectively 13 metal ion species.The result is as shown in table 2, the multiple tolerance of this bacterial strain tool, and tolerance is strong.
Embodiment 4: Pseudomonas putidaThe test of kelthane in the DCF-7 degraded tea garden soil
Bacterial strain DCF-7 30 ℃ of constant temperature culture in fermention medium were obtained the thalline fermented liquid after 24 hours.The composition of fermention medium is (g/L): Semen Maydis powder 10, bean cake powder 8, (NH
4)
2SO
46, steeping water 6, MgSO
47H
2O 1, K
2HPO
43H
2O 2, MnSO
40.05 pH 7.0.
In sterilized water, add a certain amount of kelthane (kelthane is dissolved among the acetone in advance); Behind the mixing that fully vibrates on the shaking table; Immersion soil to be tried (supplying examination soil is the red soil of pH 5.8); Make soil particle can adsorb kelthane uniformly, kelthane content is 50 mg/kg.Spray the thalline fermented liquid, stir, make that the DCF-7 cell concentration is about 5 * 10 in the soil sample
7Individual/g.Soil water content is controlled at about 60%, 25 ℃ of constant temperature.Simultaneously, with reference to people such as Li Wen research (Li W., Dai Y.; Xue B.B., Li Y.Y., Peng X.; Zhang J.S., Yan Y.C. Biodegradation and detoxification of endosulfan in aqueous medium and soil by
Achromobacter xylosoxidansStrain CS5. Journal of Hazardous Materials, 2009,167:209-216), contrast is set.Subsequently, every 5 g soil samples measuring and calculating kelthane degradation rate of getting at a distance from 7 days.
The result is as shown in Figure 3, supplies to have in the examination soil sample 84.6% kelthane to degrade in 21 days, sprays DCF-7 and can significantly promote the kelthane degradation rate, shorten the duration of degrading, and possesses practical application meaning and value.
Above-described embodiment is a kind of preferable scheme of the present invention, is not that the present invention is done any pro forma restriction, under the prerequisite that does not exceed the technical scheme that claim puts down in writing, also has other variant and remodeling.
Claims (6)
1. a strain kelthane degradation bacteria, this bacterium be pseudomonas putida (
Pseudomonas putida) bacterial strain DCF-7, on April 1st, 2012 in the preservation of Chinese common micro-organisms culture presevation administrative center, be numbered CGMCC No. 5949, this bacterium has the ability of degraded kelthane.
2. the application of the said kelthane degradation bacteria of claim 1 in receiving the reparation of kelthane contaminated soil.
3. the described application of claim 2; It is characterized in that: said bacterial strain CGMCC No. 5949 is seeded in the basic inorganic salt liquid substratum that contains kelthane 50-200 mg/L; 120 r/min concussion is cultivated more than 48 h, and described basic inorganic salt liquid substratum composition is (g/L): MgCl
20.2, NH
4NO
31, KH
2PO
42, K
2HPO
47.5 NaCl 1, deionized water 1 L.
4. the described application of claim 3 is characterized in that: described liquid culture temperature is 25-30 ℃, and the pH of basic inorganic salt liquid substratum is 5.8-7.0.
5. the described application of claim 3 is characterized in that: described liquid culture temperature is 30 ℃, and the pH of basic inorganic salt liquid substratum is 7.0.
6. the described application of claim 2; It is characterized in that: said bacterial strain CGMCC No. 5949 was seeded in the fermention medium 30 ℃ of constant temperature culture more than 24 hours; Obtain the thalline fermented liquid, degrade receiving the kelthane Contaminated soil to spray the thalline fermented liquid; The composition of fermention medium is (g/L): Semen Maydis powder 10, bean cake powder 8, (NH
4)
2SO
46, steeping water 6, MgSO
47H
2O 1, K
2HPO
43H
2O 2, MnSO
40.05 pH 7.0.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106730568A (en) * | 2016-11-22 | 2017-05-31 | 常州思宇知识产权运营有限公司 | A kind of preparation method of biological pesticide residue degrading agent |
| CN109868243A (en) * | 2019-01-30 | 2019-06-11 | 中国环境科学研究院 | Accelerate the microbial bacterial agent of organic pollution of soil reparation, preparation method and application |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1386845A (en) * | 2001-05-17 | 2002-12-25 | 蓝昆元 | Bacterium for degradating residual agricultural chemical and its preparing process |
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2012
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1386845A (en) * | 2001-05-17 | 2002-12-25 | 蓝昆元 | Bacterium for degradating residual agricultural chemical and its preparing process |
Non-Patent Citations (4)
| Title |
|---|
| 《International Journal of Integrative Biology》 20091231 Soumik Sarkar 等 "Biodegradation of Dicofol by Pseudomonas strains isolated from tea rhizosphere microflora" 第164-166页 1-6 第5卷, 第3期 * |
| 《精细化工原料及中间体》 20120305 林燕 "微生物降解有机氯农药研究进展" 第14-17、28页 1-6 , 第3期 * |
| SOUMIK SARKAR 等: ""Biodegradation of Dicofol by Pseudomonas strains isolated from tea rhizosphere microflora"", 《INTERNATIONAL JOURNAL OF INTEGRATIVE BIOLOGY》 * |
| 林燕: ""微生物降解有机氯农药研究进展"", 《精细化工原料及中间体》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106730568A (en) * | 2016-11-22 | 2017-05-31 | 常州思宇知识产权运营有限公司 | A kind of preparation method of biological pesticide residue degrading agent |
| CN109868243A (en) * | 2019-01-30 | 2019-06-11 | 中国环境科学研究院 | Accelerate the microbial bacterial agent of organic pollution of soil reparation, preparation method and application |
| CN109868243B (en) * | 2019-01-30 | 2022-02-01 | 中国环境科学研究院 | Microbial agent for accelerating soil organic matter pollution remediation, preparation method and application thereof |
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