CN106718108A - One kind carries out rapid propagation method using graft technology to cabbage - Google Patents

One kind carries out rapid propagation method using graft technology to cabbage Download PDF

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Publication number
CN106718108A
CN106718108A CN201611272812.7A CN201611272812A CN106718108A CN 106718108 A CN106718108 A CN 106718108A CN 201611272812 A CN201611272812 A CN 201611272812A CN 106718108 A CN106718108 A CN 106718108A
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China
Prior art keywords
cabbage
scion
grafting
axillary bud
stock
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CN201611272812.7A
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CN106718108B (en
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陶鹏
钟新民
李必元
岳智臣
雷娟利
赵彦婷
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Zhejiang Academy of Agricultural Sciences
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Zhejiang Academy of Agricultural Sciences
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G2/00Vegetative propagation
    • A01G2/30Grafting

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  • Life Sciences & Earth Sciences (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Cultivation Of Plants (AREA)

Abstract

Rapid propagation method is carried out to cabbage using graft technology the invention discloses one kind, first choose suitable scion and stock, using the grafting method of bud grafting method, the integrated environment of moisture-heat preservation intense light irradiation is created after grafting, and appropriate managerial is carried out after scion cabbage axillary bud turn is green, finally scion cabbage is taken root and transplanting.Tender flower stalk easily bolting in the present invention, grafting operates on shortening stem easier on rachis than grafting, and tender flower stalk easily forms side a kind of sedge, it is possible to provide more stocks sources.Using bud grafting method, relatively easy, reduction pollution flow is operated, the survival rate of scion can be improved.The cabbage axillary bud strong illumination of white is given after grafting, the axillary bud of white can be induced to form chlorophyll, so as to improve graft survival rate.Easy funnelform device of taking root is made using masking foil, moisturizing environment can be created, it is easy to root induction, and improve survival rate.

Description

One kind carries out rapid propagation method using graft technology to cabbage
Technical field
The present invention relates to vegetable grafting technical field, and in particular to one kind is carried out quickly using graft technology to cabbage The method of breeding.
Background technology
Cabbage (Brassica oleracea L.var.capitata) is Cruciferae Brassica genus annual or two Year raw herbaceous plant, is important vegetable crop.The demand growing to cabbage yield and quality to meet people, changes The economical character of good cabbage is the emphasis of breeding expert's long-term work.At present, sexual hybridization is still improvement and cultivates knot The important channel of cabbage new varieties.But cabbage belongs to typical green body vernalization type plant, the Seed sprouting stage can not Experience low temperature, palpus plant to be planted could sense when growing to a certain size to low temperature, so that bolting is bloomed again by the period of vernalization. Therefore the cabbage vernalization time is more long, and late bolting or not bolting phenomenon are frequently encountered in cabbage breeding.Not bolting If cabbage parent do not carry out specially treated, will be dead under ensuing megathermal climate.Generally to not bolting Cabbage materials carry out tissue cultures and are regenerated in the lab, and tissue-cultured seedling is transplanted into field after September part, are mesh The preceding common method for preserving the important strain of cabbage.But the cabbage in field and field carries out tissue training as explant In supporting, the pollution problem of explant often occurs, explant sterilization need to use poisonous drugs mercuric chloride, and current mercuric chloride has been public security Portion's controlled drug, obtains mercuric chloride very cumbersome.Additionally, requirement of the tissue cultures to operating personnel is higher, need to be in aseptic experiment Under the conditions of carry out.Therefore the quick breeding new method and technique of the simple and easy to do cabbage cost effective of innovation is needed badly.
Grafting (grafting), one of artificial nutrition propagation method of plant is that have callus using after plant injury Function is realized.Two forming layers for hindering face are close to and tighten together during grafting, as a result because of hyperplasia, heal into each other For the entirety that vascular tissue links together.In production practices, grafting has weight to breed improvement and the raising of economic worth Want meaning.Grafting does not allow the vegetables breeding meaning for being also easy to produce seed great some yet.Grafting can keep the excellent of scion variety Benign shape, and the advantageous feature of stock can be utilized, reach enhancing resistance, diseases and insect pests resistance.
The content of the invention
Rapid propagation method is carried out to cabbage using graft technology object of the present invention is to provide one kind, letter Easy row;The axillary bud of less cabbage is grafted onto on stock, then is taken root in suitable time points and is transplanted to field planting, So as to using axillary bud in cabbage it is more the characteristics of grafting carried out to cabbage quickly breed, the knot that solution never bolting is bloomed The preservation problem of the important strain of cabbage.
To achieve the above object, the technical scheme taken of the present invention is:
The selection of S1, scion and stock:The axillary bud of cabbage is selected as scion, it is ensured that sufficient scion comes Source, cabbage includes the axillary bud of 30 or so;The easy bolting of tender flower stalk, and easily point side a kind of sedge, and Root-stock selection tender flower stalk Rachis, it is ensured that sufficient stock source;
Preparation and grafting method before S2, grafting:Before grafting, stock material tender flower stalk is first sowed, until tender flower stalk bolting, standby With;The axillary bud of cabbage is cut into 45 degree of angles with scalpel, stock selects side a kind of sedge of main a kind of sedge or thicker;Removal stock tender flower stalk Flower, cuts 45 degree of breach at angle on suitable rachis, in the otch on axillary bud insertion rachis that will be cut, with sealing Film winds, and is fixed with clip;
S3, the suitable growth conditions of creation:The integrated environment of the constant temperature and humidity of stabilization is created, scion and stock can be promoted Between heal, improve survival rate, and give strong illumination to scion axillary bud, axillary bud can be promoted to change into green by white, axillary bud turns The green committed step for being it and surviving.
S4, growth management:After the axillary bud of cabbage turns green, topdress in time, may advantageously facilitate the growth of axillary bud, to protect Card scion receives the illumination of abundance, need to often pluck the invalid branches and leaves of stock tender flower stalk, the sunlight for preventing it from blocking scion axillary bud, The growth of scion is influenceed, clip, attention control pest and disease damage are removed after surviving;
S5, rooting induction and transplanting:After scion launches 2 blades, enclosed with the masking foil or plastic paper of width 20-30mm A funnel-form is surrounded around grafting part, to being put into matrix in masking foil and watering, after forming root, masking foil is removed, is cut and is connect Fringe is simultaneously transplanted into new seedling alms bowl.
The invention has the advantages that:
First, cabbage has fabulous affinity with tender flower stalk grafting.The cabbage of balling state contains many armpits Bud, can provide the scion source of abundance.And the easy bolting of tender flower stalk, compared to shortening stem, grafting operation is easier, and tender flower stalk is easy Side a kind of sedge is formed, so as to provide more stock sources.Because the axillary bud of cabbage is very small, cleft graft compares in grafting operation It is cumbersome, easily pollution.And bud grafting method is then relatively easy, pollution flow is reduced, substantially increase the survival rate of scion.After grafting The cabbage axillary bud intense light irradiation of white is given, the axillary bud of white can be induced to form chlorophyll, so as to improve survival rate.Use Masking foil makes easy funnelform device of taking root, and can create moisturizing environment, it is easy to root induction, and improves survival rate.
Specific embodiment
In order that objects and advantages of the present invention become more apparent, the present invention is carried out further with reference to embodiments Describe in detail.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, it is not used to limit this hair It is bright.
The embodiment of the invention provides one kind carries out rapid propagation method using graft technology to cabbage, including such as Lower step:
The selection of S1, scion and stock:The axillary bud of cabbage is selected as scion, it is ensured that sufficient scion comes Source, cabbage includes the axillary bud of 30 or so;The easy bolting of tender flower stalk, and easily point side a kind of sedge, and Root-stock selection tender flower stalk Rachis, it is ensured that sufficient stock source;
Preparation and grafting method before S2, grafting:Before grafting, stock material tender flower stalk is first sowed, until tender flower stalk bolting, standby With;The axillary bud of cabbage is cut into 45 degree of angles with scalpel, stock selects side a kind of sedge of main a kind of sedge or thicker;Removal stock tender flower stalk Flower, cuts 45 degree of breach at angle on suitable rachis, in the otch on axillary bud insertion rachis that will be cut, with sealing Film winds, and is fixed with clip;
S3, the suitable growth conditions of creation:The integrated environment of the constant temperature and humidity of stabilization is created, scion and stock can be promoted Between heal, improve survival rate, and give strong illumination to scion axillary bud, axillary bud can be promoted to change into green by white, axillary bud turns The green committed step for being it and surviving.
S4, growth management:After the axillary bud of cabbage turns green, topdress in time, may advantageously facilitate the growth of axillary bud, to protect Card scion receives the illumination of abundance, need to often pluck the invalid branches and leaves of stock tender flower stalk, the sunlight for preventing it from blocking scion axillary bud, The growth of scion is influenceed, clip, attention control pest and disease damage are removed after surviving;
S5, rooting induction and transplanting:After scion launches 2 blades, enclosed with the masking foil or plastic paper of width 20-30mm A funnel-form is surrounded around grafting part, to being put into matrix in masking foil and watering, after forming root, masking foil is removed, is cut and is connect Fringe is simultaneously transplanted into new seedling alms bowl.
The above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art For member, under the premise without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications also should It is considered as protection scope of the present invention.

Claims (1)

1. one kind carries out rapid propagation method using graft technology to cabbage, it is characterised in that comprise the following steps:
The selection of S1, scion and stock:The axillary bud of cabbage is selected as scion, it is ensured that sufficient scion source, one Individual cabbage includes the axillary bud of 30 or so;
Preparation and grafting method before S2, grafting:Before grafting, stock material tender flower stalk is first sowed, until tender flower stalk bolting, standby;With Scalpel cuts the axillary bud of cabbage into 45 degree of angles, and stock selects side a kind of sedge of main a kind of sedge or thicker;The flower of stock tender flower stalk is removed, 45 degree of breach at angle are cut on suitable rachis, in the otch on axillary bud insertion rachis that will be cut, is twined with sealed membrane Around being fixed with clip;
S3, the suitable growth conditions of creation:The integrated environment of the constant temperature and humidity of stabilization is created, and intense light irradiation is given to scion axillary bud Penetrate, axillary bud can be promoted to change into green by white;
S4, growth management:After the axillary bud of cabbage turns green, topdress in time, need to often pluck the invalid branches and leaves of stock tender flower stalk, The sunlight for preventing it from blocking scion axillary bud, influences the growth of scion, and clip, attention control pest and disease damage are removed after surviving;
S5, rooting induction and transplanting:After scion launches 2 blades, masking foil or plastic paper with width 20-30mm are around transferring Socket part position surrounds a funnel-form, and is fixed with clip, to being put into matrix in masking foil and watering, after forming root, removes tinfoil paper Paper, cuts scion and transplants into new seedling alms bowl.
CN201611272812.7A 2016-12-30 2016-12-30 Method for rapidly propagating common head cabbage by using grafting technology Active CN106718108B (en)

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107094511A (en) * 2017-07-04 2017-08-29 西南林业大学 A kind of stem apex gemmule grafting puncture method
CN108260426A (en) * 2018-03-26 2018-07-10 北京农学院 A kind of engrafting method of flower plant red sage
CN108307832A (en) * 2018-03-02 2018-07-24 浙江省农业科学院 A method of it is bloomed using grafting induction cabbage bolting under the conditions of artificial climate
CN112314360A (en) * 2020-10-13 2021-02-05 山东省农业科学院蔬菜花卉研究所 Method for grafting and breeding Chinese cabbage production seeds by using radish young sprout and Chinese cabbage young sprout
CN113950977A (en) * 2021-11-26 2022-01-21 潍坊市农业科学院(山东省农业科学院潍坊市分院) Method for improving Chinese cabbage germplasm creation efficiency

Citations (1)

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Publication number Priority date Publication date Assignee Title
CN105493907A (en) * 2016-01-27 2016-04-20 浙江省农业科学院 Cabbage small stalk grafting generation adding method utilizing tender flower stalk as stock

Patent Citations (1)

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Publication number Priority date Publication date Assignee Title
CN105493907A (en) * 2016-01-27 2016-04-20 浙江省农业科学院 Cabbage small stalk grafting generation adding method utilizing tender flower stalk as stock

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张俊朴著: "《园林植物开发研究》", 30 June 1995, 中国城市出版社 *
高国华等著: "《家庭花卉病虫害防治》", 31 January 2004, 天津科学技术出版社 *

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107094511A (en) * 2017-07-04 2017-08-29 西南林业大学 A kind of stem apex gemmule grafting puncture method
CN107094511B (en) * 2017-07-04 2019-05-28 西南林业大学 A kind of stem apex gemmule grafting puncture method
CN108307832A (en) * 2018-03-02 2018-07-24 浙江省农业科学院 A method of it is bloomed using grafting induction cabbage bolting under the conditions of artificial climate
CN108260426A (en) * 2018-03-26 2018-07-10 北京农学院 A kind of engrafting method of flower plant red sage
CN112314360A (en) * 2020-10-13 2021-02-05 山东省农业科学院蔬菜花卉研究所 Method for grafting and breeding Chinese cabbage production seeds by using radish young sprout and Chinese cabbage young sprout
CN113950977A (en) * 2021-11-26 2022-01-21 潍坊市农业科学院(山东省农业科学院潍坊市分院) Method for improving Chinese cabbage germplasm creation efficiency

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