CN108260426A - A kind of engrafting method of flower plant red sage - Google Patents

A kind of engrafting method of flower plant red sage Download PDF

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CN108260426A
CN108260426A CN201810250237.3A CN201810250237A CN108260426A CN 108260426 A CN108260426 A CN 108260426A CN 201810250237 A CN201810250237 A CN 201810250237A CN 108260426 A CN108260426 A CN 108260426A
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plant
scion
grafting
stock
red sage
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CN108260426B (en
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陈洪伟
孔维
孔维一
刘笑冰
王红利
葛秀秀
刘克锋
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Beijing University of Agriculture
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Beijing University of Agriculture
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Abstract

The invention discloses a kind of engrafting method of flower plant red sage, this method is included using the red sage plant in seedling stage as scion, grafts in Zhu lip plant in seedling stage.The engrafting method of red sage is the distant grafting for salvia in the present invention, and the growth potential and resistance of scion are improved using outstanding stock, and enhances grafting and Zhu's lip inter-species Wide_cross in Plant compatibility, improves the setting percentage of hybridization.

Description

A kind of engrafting method of flower plant red sage
Technical field
The invention belongs to ornamental plant heteroplastic graft technical fields, are related to belonging to the grafting between flower plant not of the same race Method, more particularly to flower plant red sage and Zhu's lip engrafting method.
Background technology
Grafting is a kind of ancient plant asexual multiplication technology, is one kind of the vegetative reproduction in vegetative propagation, is a kind of Ancient artificial propagation plant method.Grafting common engrafting method mainly has scion grafting, bud to connect, wherein scion grafting be divided into as cut-grafting and Cleft grafting.
Grafting is carried out using the function with callus after plant injury, i.e., the branch or bud of a kind of plant, grafting Onto the stem or root of another plant, be connected together two parts is made to grow up to a complete plant.It should make to connect during grafting The forming layer of fringe and stock is combined closely, to ensure that scion survives.The branch or bud connected, is called scion;The plant connect, It is called stock or platform wood.The seedling of 2 to 4 buds of tool is generally selected in scion, becomes the top or top of plant after grafting, and stock is transferred Become the root system part of plant after connecing.
During grafting, make two forming layers for hindering face close and tighten together, as a result because of hyperplasia, be healing each other to The entirety that vascular tissue links together.The principal element for influencing grafting survival is the affinity of scion and stock, secondly It is the management after the technology and grafting grafted.So-called affinity is exactly scion and stock in internal organizational structure, physiology and something lost It catches, it is mutually the same or close, so as to the ability being combined with each other.Affinity is high, high graft survival rate.Conversely, then Survival rate is low.In general, plant affiliation is nearer, then affinity is stronger.
Red sage (Salvia splendens) is also known as that firecracker is red, ivory is red, is Lamiaceae Salvia platymiscium, and inflorescence is repaiied Long, pattern is bright-coloured, and the florescence is again long, adaptable, for the herbage flower most generally cultivated in Chinese city and gardens.Like it is warm and Sunny environment.It can not resist cold, resistance to half is cloudy, avoids frost and snow and high temperature, is afraid of ponding and basic soil.The fruit of red sage is small heavily fortified point Fruit, ellipse, includes black seeds, easy to fall off.
The propagation method generally use sowing of red sage or cuttage breeding, it is more to sow.North China's sowing season Unlimited, remaining area is advisable with spring.The red sage florescence is slower, and spring sowing person is bloomed for 9~October, such as to make advance flowering period or harvesting Seed should be sowed at greenhouse or hotbed by seed at the beginning of 3 months.A small amount of base manure is imposed in seed bed, it is bed surface is smooth and sprinkle profoundly water, water It is sowed after oozing, covers one layer of thin soil, seed is sprouted within after planting 8~10 days.Growth is bloomed for about 100 days, about two months florescence.Cuttage is more It is more suitable with 3~May or 9~October with spray, it is carried out with reference to terminal bud is plucked.
Zhu's lip (Salvia coccinea) is the plant that Lamiaceae Salvia belongs to, and annual herb flowers, leaf length is heart-shaped, Leaf margin has crenation, raceme basidixed.Property happiness it is warm on the sunny side environment, 15 DEG C to 30 DEG C of adaptability temperature, preferably in fertile sandy loam Middle growth.Flower basidixed, pattern enrich, and ground plants 50 centimetres to 60 centimetres of plant height, and plant is plentiful, shrub shape, leaf bottle green, and flower-shape is Horn-like, color is beautiful, and the florescence is early, and heat-resisting and drought-resistant property is good.Zhu's lip landscape application effect is good, available for arrangement flower bed or flower Border, also can group planting among lawn.
The happiness of Zhu's lip warm environment, 15 DEG C to 30 DEG C of adaptability temperature on the sunny side are preferably grown in fertile sandy loam.Ground plants plant height 50 centimetres to 60 centimetres, heat-resisting and drought-resistant property is good.9 weeks to 11 weeks are needed under ideal temperature.Seedling Stage is 4 weeks to 5 weeks, field planting 5 weeks to 6 weeks are needed after upper basin.Zhu's lip generally use seed broadcasts breeding.Potting requirement:Medium requirements are loose ventilative, well-drained Matrix can be matched with+20% perlite of+30% garden mould of 50% turf, it is desirable that the pH value of matrix is that 5.5 to 5.8, EC values are 1.0 to 1.5.Container selection preferably selects 10 centimetres to 12 centimetres of basin device.
Red sage and Zhu's lip belong to two kinds of herbaceous ornamentals that Lamiaceae Salvia belongs to not of the same race.Wherein Zhu's lip plant is whole Body tool fragrance, growth potential, resistance, setting percentage are stronger compared with red sage, by the way that the two grafting can be improved to the growth potential of red sage, and Improve the compatibility of the two distant hybridization.But both plant grafts are inter-species distant grafting, are belonged between not kindred plant Physiological structure and inherent cause difference, cause plant tissue compatibility low, the speed of wound healing between organizing is slow, causes to transfer It is relatively low to be connected into motility rate, grafting difficulty is high.
And flower plant red sage and Zhu's lip distant hybridization have an apparent dysgenesia, and the present invention is by by red sage and Zhu Grafting red sage/Zhu's lip after lip grafting hybridizes again with Zhu's lip, is reduced dysgenesia between the two close to method by physiology Or it eliminates, hence it is evident that improve the distant hybridization obstacle between red sage and Zhu's lip, enhance the compatibility between two kinds of plants, it is remote so as to improve The success rate of edge hybridization, the setting percentage after grafting hybridizes with Zhu's lip significantly improve.
Invention content
The purpose of the present invention is being directed to technological deficiency present in existing red sage cultivating process, a kind of ornamental flower is provided The engrafting and cultivating method of red sage, grafting red sage/Zhu's lip and the stock Zhu lip of the method for the present invention engrafting and cultivating are bloomed simultaneously, Appreciation effect is improved, while improves the growth potential and resistance of scion, the plant leaf chlorophyll content after grafting significantly improves, light It closes rate to be obviously improved so that the Nutrition and Metabolism state of scion is improved;And the pollen morphology of grafting generates variation, Mutual intermolecular hybrid affinity enhancing, enhances grafting and Zhu's lip inter-species Wide_cross in Plant compatibility, improves the knot of hybridization Real rate.
Purpose to realize the present invention, one aspect of the present invention provides a kind of engrafting and cultivating method of red sage, including with seedling The red sage plant of phase is scion, grafts in Zhu lip plant in seedling stage.
Wherein, the seedling stage red sage is to grow to the red sage plant of 5~6 pairs of true leaves;Seedling stage Zhu lip is growth To Zhu lip plant of 5~6 pairs of true leaves.
Particularly, the seedling stage red sage is the red sage do not bloomed;Seedling stage Zhu lip is Zhu's lip do not bloomed.
Especially, the seedling stage red sage is grows to 5~6 pairs of true leaves, and the red sage plant not bloomed;The seedling stage Zhu's lip is grows to 5~6 pairs of true leaves, and Zhu lip plant not bloomed.
Particularly, the red sage plant in the seedling stage is prepared as follows:Red sage is sowed in hole tray first, is treated After red sage grows to 2~3 pairs of true leaves, basin is changed, then culture grows to 5~6 pairs of true leaves;Zhu's lip plant in the seedling stage according to It is prepared by following method:Zhu's lip is sowed first in hole tray, after Zhu's lip grows to 2~3 pairs of true leaves, changes basin, then culture growth To 5~6 pairs of true leaves.
Particularly, it further includes using the red sage plant clip in the seedling stage its top tender stem 1~3 section as scion;By institute Zhu lip plant for stating seedling stage cuts plant upper end, and the lower part 3~4 for retaining Zhu's lip plant stem section is saved, as graft stock.
Wherein, the scion is preferably to retain the red sage top tender stem of 2~3 sections;The stock retains the most upper section of stem section A pair of of functional leaf, rest blade, lateral bud and root turion bud remove.
Particularly, the distance of the functional leaf of final section that the lower end distance of the scion counts from top to bottom is more than 0.5cm, preferably 2~3cm, further preferably 2~2.5cm are still more preferably 2cm.
Especially, the functional leaf of the scion removal 1/3~2/3.
Particularly, retain each functional leaf of scion, and on each functional leaf shear removal 1/3~2/3 face Product preferably removes the blade of 1/2 area.
Particularly, it further includes and the lower part of scion red sage top tender stem is whittled into wedge shape, then carry out the grafting again Processing.
Especially, the length of the wedge shape is 0.5~1.0cm, further preferably preferably 0.7~1.0cm, 1.0cm.
Wherein, Zhu's lip in the seedling stage as stock is grafted in using the red sage plant in seedling stage as scion using cleft graft On plant.
Particularly, scion is inserted into the depth of stock Zhu lip plant as 0.5~1.0cm, preferably 0.7~1.0cm, further Preferably 1.0cm.
Especially, in the cleft graft grafting procedures, the depth of riving of seedling stage Zhu's lip as stock for 0.5~ 1.0cm, preferably 0.7~1.0cm, further preferably 1.0cm.
Another aspect of the present invention provides a kind of engrafting method of flower plant red sage, the step carried out including order below Suddenly:
1) scion selects;
Clip stalwartness plant red sage top tender stem removes 1/3~2/3 functional leaf as scion;
2) Root-stock selection:
The healthy and strong plant Zhu's lip of selection cuts off the upper end of plant, and the lower part 3~4 for retaining Zhu's lip plant stem section is saved, as anvil Wood;
3) grafting is handled:
In the gap of stem section upper end that red sage scion lower end insertion stock is longitudinally rived using cleft graft, and to connect The bast of fringe is aligned with stock bast;Then the engaging portion of scion and stock is snarled using bondage material, package is secured;
4) managing after grafting
After the completion of grafting, water and fertilizer management is carried out, lateral bud and root evil bud that stock is sprouted is trimmed in time, obtains grafting a string Red/Zhu's lip.
Wherein, scion described in step 1) is to retain the top tender stem of 1~3 section.
Particularly, scion described in step 1) is preferably to retain the top tender stem of 2~3 sections.
Especially, the distance of the functional leaf of final section that the lower end distance of the scion counts from top to bottom is more than 0.5cm, preferably 2~3cm, further preferably 2~2.5cm are still more preferably 2cm.
Particularly, the functional leaf of the scion removal 1/3~2/3 refers to the area and functional leaf of the functional leaf of removal The ratio of the original area of piece is 1/3~2/3, preferably 1/2.
Especially, retain each functional leaf of scion, and on each functional leaf shear removal 1/3~2/3 face Product preferably removes the blade of 1/2 area.
Particularly, step 1A is further included), scion lower part is whittled into wedge shape, then carries out the grafting processing again.
Especially, the length of the wedge shape is 0.5~1.0cm, further preferably preferably 0.7~1.0cm, 1.0cm.
Wherein, the functional leaf of scion shear removal described in step 1) 1/2.
Wherein, stock described in step 2) retains a pair of of functional leaf of the most upper section of stem section, rest blade, lateral bud and root turion bud Removal.
Particularly, the lower part 3 of Root-stock selection reservation Zhu's lip plant stem section is saved, as stock.
Wherein, the length of the scion lower end in stock is inserted into described in step 3) as 0.5~1.0cm, preferably 0.7~ 1.0cm, further preferably 1.0cm.
Particularly, red sage scion lower end is whittled into length as 0.5~1.0cm, preferably 0.7~1.0cm, it is further excellent After being selected as the wedge shape of 1.0cm, it is inserted into the gap for the stock longitudinally rived.
Wherein, the gap depth of stem section upper end that stock described in step 3) is longitudinally rived is 0.5~1.0cm, preferably 0.7~1.0cm, further preferably 1.0cm.
Particularly, further included in step 4) and cover grafting with valve bag, and in temperature be 18~25 DEG C, relative humidity It is cultivated under conditions of being 70%~80%.
Especially, the bondage material selection sealed membrane described in step 3), ' Parafilm ' sealed membrane (U.S. preferably Bemis companies produce).
The method of the present invention has the following advantages that:
1st, the present invention using Zhu's lip as stock, the well developed root system growth potential of Zhu's lip is strong, ensure that grafting survive after battalion Support supply.
2nd, the method for the present invention, which greatly improved, belongs to not kindred plant red sage and the grafting success rate of Zhu's lip, grafting survival Rate reaches more than 43%, accelerates the speed of growth of the grafting after grafting, and the plant height of grafting is high, and functional leaf blade area is big, Stem section is thick.
3rd, the photosynthetic capacity of plant Zhu lip is strong, and chlorophyll content in leaf blades is higher than red sage, after scion survives, passes through guarantor It stays stock axillary bud that stock can be made to bloom simultaneously with scion, forms the different colored moulding of homophyletic, increase the ornamental value of flower plant.
4th, the method for the method of the present invention red sage grafting Zhu's lip, easy to operate, survival rate is high, suitable for wide popularization and application.
5th, the method for the present invention is improved the moisturizing of grafting, and use ' Parafilm ' sealed membrane is bondage material, should Material is a kind of thermoplasticity self-sealing film, has good ductility, and stretchable 3~4 times are not broken, has certain adhesion strength, There is fabulous moisture vapor permeability simultaneously, when ensureing normal oxygen, carbon dioxide stream passes, lock moisture.In addition it is grafting Afterwards, it is covered together with basin with valve bag, scattering and disappearing for moisture can be effectively reduced, improve the humidity of the grafting part of grafting, improved Graft survival rate.
Preservative film, silk thread, Grafting clip being used to carry out in grafting binding site from usually, binding is different, and present invention selection has There is the bondage that the sealed membrane of fine air permeability and moisture retention carries out wound to fix, the external world that can effectively reduce grafting plant junction is dirty Dye improves the mutual binding force in engaging portion.
6th, the chlorophyll content for the grafting functional leaf that the method for the present invention obtains is high, and Net Photosynthetic Rate is high, improves grafting The photosynthetic capacity of plant, grafting plant is tall and big, healthy and strong, stem is thick, and flower amount is more, inflorescence is full.
7th, plant stock and scion can bloom simultaneously after the method for the present invention grafting, and ornamental value, novelty are improved, and By later stage full-bloom stage distant hybridization, it is found that grafting plant increases for the recognition capability of stock pollen, significantly improve The setting percentage that red sage hybridizes with Zhu's lip improves the distant hybridization grafting affinity of red sage.
Description of the drawings
Fig. 1 is red sage scion figure of the present invention;
Fig. 2 is red sage scion lower end enlarged drawing of the present invention;
Fig. 3 schemes for grafting survival plant;
Fig. 4 is bloomed figure simultaneously for stock, scion.
Specific embodiment
The invention will now be further described with reference to specific embodiments, the advantages and features of the present invention will be with description and It is apparent.But these embodiments are only exemplary, do not form any restrictions to the scope of the present invention.People in the art Member it should be understood that without departing from the spirit and scope of the invention can to the details of technical solution of the present invention and form into Row modifications or substitutions, but these modifications and replacement are each fallen in protection scope of the present invention.
Tool, material used in following embodiments etc. are commercially available unless otherwise specified.
Plant red sage material is red sage ' prince charming in the embodiment of the present invention ', Zhu's lip material is Zhu's lip ' red cloud ', Two kinds of Breeding growing environments are identical, and selection period is seedling stage (plant before not blooming carries out grafting processing).
The bondage materials'use U.S. Bemis companies ' Parafilm ' sealed membrane of grafting of the present invention.
Embodiment 1
1st, the preparation of stock, scion material
Zhu's lip seed, red sage seed are in the same period sowed in different hole trays respectively, and matrix is covered with vermiculite Surface, wherein using volume ratio as 1:The turf of 1 ratio mixing is cultivation matrix with sand, then is protected with covered rearing with plastic film hole tray It is wet;Basin (purpose is to promote root growth, preferably plant can be promoted integrally to grow) is changed when length is to 2~3 pairs of true leaves, is noted simultaneously Meaning water and fertilizer management (pours weekly one time 1:' Christmas tree ' water soluble composite fertilizer of 1000 ratios dissolving), treat that Zhu's lip, red sage change basin When growing to 5~6 pairs of true leaves, grafted.
Length can be grafted to during 5~6 pairs of true leaves as stock after Zhu's lip changes basin, because removing after top 2 is saved can also stay Lower 3~4 section is of convenient length as stock;Red sage is grown to during 5~6 pairs of true leaves after changing basin and scion can be used as to draw materials, because 5~6 During to true leaf, 2~3 stem segment rugosity of top is suitable.
2nd, stock material is prepared
2A, seedling stage is chosen, Zhu lip stalwartness plant with 5~6 pairs of true leaves cuts off plant upper end, retains Zhu's lip plants stems Section subordinate portion 3 saves (being usually 3~4 sections), is protected as stock, and by a pair of of functional leaf of the most upper section in the stem section portion of reservation It stays, for carrying out photosynthesis, provides Nutrition and Metabolism product for scion and stock itself, rest blade, lateral bud and root turion bud are gone Remove, prevent these separate living tissues from fighting for scion nutrition so that the nutrition of stock can fully be delivered to scion part, improve scion into Motility rate.
2B, longitudinal stock stem section upper end of riving, depth 1.0cm (usually 0.5~1.0cm, preferably 0.7~ 1.0cm), stock material is made, it is spare.
A pair of of the functional leaf for retaining a most upper section for stock in the present invention carries out photosynthesis, but side excessive in order to prevent Bud is sprouted therefore only retains a pair of of functional leaf.
3rd, scion material is prepared
3A, seedling stage is chosen, the red sage stalwartness plant with 5~6 pairs of true leaves, clip retains 2 sections (being usually 1~3 section) Top tender stem, and the distance of functional leaf that saves of distance second for 2cm (be usually 2~3cm) top tender stem as scion, Then shear removal partial function leaf again, the area of the functional leaf of reservation be former function area 1/2 (be usually 1/3~2/3, Preferably 1/2) the i.e. functional leaf of one semi-area of shear removal, that is, by functional leaf blade area shear removal half, such as scheme 1;
Preferably retain each functional leaf of scion, shear removal 1/3~2/3, preferably 1/2 on each functional leaf The blade of area.
Half of cutting off the functional leaves area or so, reduce scion transpiration dehydration, improve grafting success rate and grafting into Motility rate.
Scion retains 1~3 section top tender stem in the present invention, and retaining 1 section growth can be slow, and bud quantity is few, sprouts The small survival rate of probability can reduce;If retaining 3 sections, the mutual spacing of section is small, and easy dehydration if long, survival rate can reduce; Retain 2 to save, the quantity of bud, the length of scion is relatively mild.
3B, it is stem is whittled into length at a pair of (i.e. Section 2) functional leaf lower section 1cm in the bottom of scion tender stem The wedge shape of 1.0cm (being usually 0.5~1.0cm, preferably 0.7~1.0cm), is made scion material, spare, such as Fig. 1,2.
It after the completion of stock, scion material preparation, combines as early as possible, prevents the bevel oxidation of the two, pay attention to the works such as scissor blade The disinfection of tool.
4th, grafting is handled
Scion consistent with stock thickness or slightly thin is chosen, and stock cleavage is inserted into the scion wedge shape lower part cut Point, and the bast of scion is aligned with stock bast, then with the sealed membrane (U.S. for being about 3cm wide about 1.5cm Bemis companies ' Parafilm ' sealed membrane) engaging portion of scion and stock is snarled, package is secured.
The embodiment of the present invention uses ' Parafilm ' sealed membrane as bondage material, the engaging portion of bondage grafting, ' Parafilm ' sealed membrane has good gas permeability and moisture retention, effectively reduces the outside contamination of grafting plant junction, improves The mutual binding force in engaging portion.Other than ' Parafilm ' sealed membrane, other sealed membranes are suitable for the present invention, this field In other existing known bondage materials be suitable for the present invention, such as food fresh keeping membrane can replace to a certain extent.
Red sage scion and Zhu's lip stock should be grafted in seedling stage, and when grafting pays attention to scion and the bast alignment of stock.Anvil Wooden Zhu's lip in seedling stage after, xylem increases in stem, grafting be not easy to survive.Select the progress that xylem is less in Zhu's lip stock stem Grafting.
5th, the culture of grafting
It after the completion of grafting, is sprinkled profoundly water to plant, and grafting is covered with valve bag, keep moisture.Grafting is placed in 18 ~25 DEG C, the culture of humidity 70%~80%, daily early morning opens valve bag turn-over, and removes the lateral bud and root turion of sprouting in time Then bud covers plant again, plant survives within 15 days or so, removes valve bag.
Selection Zhu lip, each 30 plants of red sage carry out grafting processing respectively, count survival rate, statistical result is as shown in table 1.
Embodiment 2
1st, the preparation of stock, scion material
It is same as Example 1.
2nd, stock material is prepared
In addition to step 2A) in retain lip plant stem section subordinate Zhu portion 4 and save as other than stock, remaining and 1 phase of embodiment Together.
3rd, scion material is prepared
3A, seedling stage is chosen, the red sage stalwartness plant with 5~6 pairs of true leaves, clip retains the top tender stem of 3 sections, and The distance of functional leaf apart from Section 3 be the top tender stem of 2.5cm as scion, then shear removal partial function leaf again, guarantor The area of the functional leaf stayed be former function area 1/2 (be usually 1/3~2/3, preferably 1/2) i.e. one semi-area of shear removal Functional leaf, that is, by functional leaf blade area shear removal half;
3B, stem is being whittled into length at 1.5cm below a pair of (i.e. Section 3) functional leaf in the bottom of scion tender stem For the wedge shape of 1.0cm, scion material is made, it is spare.
4th, grafting is handled
It is same as Example 1.
5th, the culture of grafting
It is same as Example 1.
Selection Zhu lip, each 30 plants of red sage carry out grafting processing respectively, count survival rate, statistical result is as shown in table 1.
Embodiment 3
1st, the preparation of stock, scion material
It is same as Example 1.
2nd, stock material is prepared
In addition to step 2A) in retain lip plant stem section subordinate Zhu portion 3 and save as stock, stock stem section of longitudinally riving upper end, Except depth 0.7cm, remaining is same as Example 1.
3rd, scion material is prepared
3A, seedling stage is chosen, the red sage stalwartness plant with 5~6 pairs of true leaves, clip retains the top tender stem of 2 sections, and The distance of functional leaf apart from Section 2 be the top tender stem of 2.0cm as scion, then shear removal partial function leaf again, guarantor The area of the functional leaf stayed be former function area 1/2 (be usually 1/3~2/3, preferably 1/2) i.e. one semi-area of shear removal Functional leaf, that is, by functional leaf blade area shear removal half;
3B, stem is being whittled into length at 1.3cm below a pair of (i.e. Section 2) functional leaf in the bottom of scion tender stem For the wedge shape of 0.7cm, scion material is made, it is spare.
4th, grafting is handled
It is same as Example 1.
5th, the culture of grafting
It is same as Example 1.
Selection Zhu lip, each 30 plants of red sage carry out grafting processing respectively, count survival rate, statistical result is as shown in table 1.
Embodiment 4
1st, the preparation of stock, scion material
It is same as Example 1.
2nd, stock material is prepared
In addition to step 2A) in retain lip plant stem section subordinate Zhu portion 3 and save as stock, stock stem section of longitudinally riving upper end, Except depth 0.5cm, remaining is same as Example 1.
3rd, scion material is prepared
3A, seedling stage is chosen, the red sage stalwartness plant with 5~6 pairs of true leaves, clip retains the top tender stem of 2 sections, and The distance of functional leaf apart from Section 2 be the top tender stem of 2.0cm as scion, then shear removal partial function leaf again, guarantor The area of the functional leaf stayed be former function area 2/3 (be usually 1/3~2/3, preferably 1/2) i.e. 1/3 area of shear removal Functional leaf, that is, by functional leaf blade area shear removal 1/3;
3B, stem is being whittled into length at 1.5cm below a pair of (i.e. Section 2) functional leaf in the bottom of scion tender stem For the wedge shape of 0.5cm, scion material is made, it is spare.
4th, grafting is handled
It is same as Example 1.
5th, the culture of grafting
It is same as Example 1.
Selection Zhu lip, each 30 plants of red sage carry out grafting processing respectively, count survival rate, statistical result is as shown in table 1.
Embodiment 5
1st, the preparation of stock, scion material
It is same as Example 1.
2nd, stock material is prepared
It is same as Example 1.
3rd, scion material is prepared
3A, seedling stage is chosen, the red sage stalwartness plant with 5~6 pairs of true leaves, clip retains the top tender stem of 3 sections, and The distance of functional leaf apart from Section 3 be the top tender stem of 2.0cm as scion, then shear removal partial function leaf again, guarantor The area of the functional leaf stayed is 1/2 (being usually 1/3~2/3) the i.e. functional leaf of 1/2 area of shear removal of former function area, Namely by functional leaf blade area shear removal 1/2;
3B, stem is being whittled into length at 1.0cm below a pair of (i.e. Section 3) functional leaf in the bottom of scion tender stem For the wedge shape of 1.0cm, scion material is made, it is spare.
4th, grafting is handled
It is same as Example 1.
5th, the culture of grafting
It is same as Example 1.
Selection Zhu lip, each 30 plants of red sage carry out grafting processing respectively, count survival rate, statistical result is as shown in table 1.
1 Zhu's lip stock grafting seedling of table and own-rooted tree growth and development overall target test result
Embodiment 6
In addition to surviving after grafting 15 days in step 5) retains the lateral bud of stock in itself, remaining side sprouted in plant Bud is gone in addition, remaining is same as Example 1, removes the lateral bud of stock sprouting to ensure that the nutrition of plant whole is only supplied to guarantor Stay lateral bud and scion, growth period pay attention to removing should not lateral bud and root turion bud, apply primary ' Christmas tree ' Water Soluble Compound weekly Fertilizer.The grafting plant that acquisition scion and stock are bloomed simultaneously, as shown in Figure 4.
Reference examples 1
Red sage own-rooted tree is sowed using in 1 hole tray of embodiment as reference examples 1, water and fertilizer management and the phase of embodiment 1 Together.
Reference examples 2
Zhu's lip own-rooted tree is sowed using in 1 hole tray of embodiment as reference examples 2, water and fertilizer management is identical with embodiment 1.
The growth and development overall target test of 1 red sage grafting of test example
Grafting red sage/Zhu's lip of embodiment 1 and the own-rooted tree red sage of reference examples 1, own-rooted tree Zhu's lip of reference examples 2 The plant height of plant is measured when growing to full-bloom stage;Stem is thick;It is complete that plant top is measured using YMJ~CH intelligence leaf areas measuring system The leaf area of full expansion functional leaf;The inflorescence fresh weight of the complete blooming flower sequence in plant top;
According to《(Li Hesheng chief editors, go out at Higher Education Publishing House for 2000 for plant physiology and biochemistry test principle and technology Version)》Method measure chlorophyll content;
Using the photosynthesis measurement system (Lufthansa scientific instrument Co., Ltd) of CIRAS~3, continuous 5 days at the morning 10 Net Photosynthetic Rate is measured, clamping plant top first segment expansion functional leaf with leaf chamber measures, and every plant repeats three times, is averaged.
Test result is as shown in table 2:
2 Zhu's lip stock grafting seedling of table and red sage, Zhu's lip own-rooted tree growth and development overall target test result
Note:Numerical value represents mean+/-standard error (n=5), and different letters represent not existing together at the same time in table Significant difference (P < 0.05) between reason
Test result shows:Grafting significantly improves the growth and development overall target of red sage, promotes plant chlorophyll Synthesis, improve the photosynthetic rate of plant.Because the chlorophyll content of stock Zhu's lip is high, photosynthetic capacity is strong, by transferring Effect is connect, the merit of stock Zhu's lip influences the growth and development of scion, so as to improve grafting chlorophyll content and light Close ability to function.
The cross experiment of 2 red sage grafting of test example
When grafting red sage/Zhu's lip of example 1 to be performed and the own-rooted tree red sage of reference examples 1 grow to full-bloom stage, choosing 5 little Hua that the inflorescence middle and lower part speed of growth is identical, remaining little Hua is taken to remove.When the corolla for choosing little Hua is isometric with calyx tube Emasculation is entangled with sulfuric acid paper bag, after flower gynoecium stretches out corolla apex bifurcated, is collected fresh male parent (Zhu's lip) pollen, is used tweezer Sub-folder lives filigree, and artificial pollination is carried out to column cap bifurcated tip, and morning pollination repeats primary, bagging note of pollinating primary rear afternoon Pollination date, time are recorded, is placed in ventilation area without shade, after stylet headdress flower hat wilts and comes off, draping bag entangles inflorescence, pollination knot Shu Hou can spray solid plant foliar fertilizer, promote seed maturity, after calyx decoloration is dried, acquisition pollination inflorescence, and threshing, Cross-fertile number is counted, calculates setting percentage, wherein:Setting percentage=(solid number/pollination little Hua numbers) × 100%.
Pollination, solid statistics result of calculation such as table 3.
3 Zhu's lip stock grafting seedling of table and own-rooted tree pollination, setting percentage test result
3 test result of table shows:Grafting Distant crossing siliqua-bearing rate has significantly compared with own-rooted tree Distant crossing siliqua-bearing rate Difference illustrates that the recognition capability of plant pair stock pollen can be improved by grafting processing, can to a certain extent abolish a string Red and Zhu's lip distant hybridization obstacle, so as to improve the setting percentage of red sage and Zhu's lip plant distant hybridization.

Claims (10)

1. a kind of engrafting method of flower plant red sage, which is characterized in that including using the red sage plant in seedling stage as scion, transferring It is connected to Zhu lip plant in seedling stage.
2. engrafting method as described in claim 1, which is characterized in that the seedling stage red sage is to grow to 5~6 pairs of true leaves Red sage plant.
3. engrafting method as claimed in claim 1 or 2, which is characterized in that seedling stage Zhu lip is grows to 5~6 pairs of true leaves Zhu lip plant.
A kind of 4. engrafting method of flower plant red sage, which is characterized in that the step of being carried out including order below:
1) scion selects:
Clip stalwartness plant red sage top tender stem removes 1/3~2/3 functional leaf as scion;
2) Root-stock selection:
The healthy and strong plant Zhu's lip of selection cuts off the upper end of plant, and the lower part 3~4 for retaining Zhu's lip plant stem section is saved, as stock;
3) grafting is handled:
In the gap of stem section upper end that red sage scion lower end insertion stock is longitudinally rived using cleft graft, and cause scion Bast is aligned with stock bast;Then the engaging portion of scion and stock is snarled using bondage material, package is secured;
4) managing after grafting:
After the completion of grafting, water and fertilizer management is carried out, the lateral bud and root evil bud that trimming stock is sprouted in time, acquisition grafting red sage/ Zhu's lip.
5. engrafting method as claimed in claim 4, which is characterized in that scion described in step 1) is to retain the top of 1~3 section Tender stem.
6. engrafting method as described in claim 4 or 5, which is characterized in that the work(of scion shear removal described in step 1) 1/2 It can leaf.
7. engrafting method as described in claim 4 or 5, which is characterized in that stock described in step 2) retains the most upper section of stem section A pair of of functional leaf, rest blade, lateral bud and root turion bud remove.
8. engrafting method as described in claim 4 or 5, which is characterized in that be inserted under the scion in stock described in step 3) The length at end is 0.5~1.0cm, further preferably preferably 0.7~1.0cm, 1.0cm.
9. engrafting method as described in claim 4 or 5, which is characterized in that the stem section that stock described in step 3) is longitudinally rived The gap depth of upper end is 0.5~1.0cm, further preferably preferably 0.7~1.0cm, 1.0cm.
10. engrafting method as described in claim 4 or 5, which is characterized in that further included in step 4) by grafting valve bag It covers, and is cultivated under conditions of temperature is 18~25 DEG C, relative humidity is 70%~80%.
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109429758A (en) * 2018-12-24 2019-03-08 遵义县秋之丰园林绿化有限公司 A kind of screening test method of Mexico Salvia japonica graft stock

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103181291A (en) * 2013-04-02 2013-07-03 镇江瑞繁农艺有限公司 Cultivation and selection method of red sage seeds with high germination rate
CN106561288A (en) * 2015-10-08 2017-04-19 戚友亦 Method for grafting of chrysanthemum with Artemisia lavandulaefolia
CN106613659A (en) * 2016-12-14 2017-05-10 河南红枫种苗股份有限公司 Grafting cultivation method for euonymus golden armors
CN106718108A (en) * 2016-12-30 2017-05-31 浙江省农业科学院 One kind carries out rapid propagation method using graft technology to cabbage

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103181291A (en) * 2013-04-02 2013-07-03 镇江瑞繁农艺有限公司 Cultivation and selection method of red sage seeds with high germination rate
CN106561288A (en) * 2015-10-08 2017-04-19 戚友亦 Method for grafting of chrysanthemum with Artemisia lavandulaefolia
CN106613659A (en) * 2016-12-14 2017-05-10 河南红枫种苗股份有限公司 Grafting cultivation method for euonymus golden armors
CN106718108A (en) * 2016-12-30 2017-05-31 浙江省农业科学院 One kind carries out rapid propagation method using graft technology to cabbage

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
张美玲,陈洪伟,王红利,石爱平,洪培培,刘克锋: "植物远缘杂交育种现状与展望", 《安徽农业科学》 *
花百科小琪: "一串红一花多色怎么嫁接", 《WWW.HUABAIKE.COM/YHJQ/7687.HTML》 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109429758A (en) * 2018-12-24 2019-03-08 遵义县秋之丰园林绿化有限公司 A kind of screening test method of Mexico Salvia japonica graft stock

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