CN106715723B - 测定样品中pik3ca突变状态的方法 - Google Patents

测定样品中pik3ca突变状态的方法 Download PDF

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CN106715723B
CN106715723B CN201580052321.9A CN201580052321A CN106715723B CN 106715723 B CN106715723 B CN 106715723B CN 201580052321 A CN201580052321 A CN 201580052321A CN 106715723 B CN106715723 B CN 106715723B
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pik3ca
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CN106715723A (zh
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艾维克雷亚·里安尼多
阿西娜·马克
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CN201580052321.9A 2014-08-07 2015-07-28 测定样品中pik3ca突变状态的方法 Active CN106715723B (zh)

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US201462034231P 2014-08-07 2014-08-07
US62/034,231 2014-08-07
PCT/GR2015/000036 WO2016020710A1 (en) 2014-08-07 2015-07-28 Method of determining pik3ca mutational status in a sample

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Families Citing this family (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES3038392T3 (en) 2010-05-14 2025-10-13 Massachusetts Gen Hospital Neoantigen specific cytotoxic t cells for use in treating cancer
WO2015039024A1 (en) 2013-09-16 2015-03-19 Oregon Health & Science University Bioabsorbable clips and applicator for tissue closure
WO2015077717A1 (en) 2013-11-25 2015-05-28 The Broad Institute Inc. Compositions and methods for diagnosing, evaluating and treating cancer by means of the dna methylation status
US11725237B2 (en) 2013-12-05 2023-08-15 The Broad Institute Inc. Polymorphic gene typing and somatic change detection using sequencing data
JP7060324B2 (ja) 2013-12-20 2022-04-26 ザ・ブロード・インスティテュート・インコーポレイテッド ネオ抗原ワクチンによる併用療法
BR112017009196B1 (pt) 2014-11-03 2021-12-14 Oregon Health & Science University Clipe cirúrgico, conjunto de clipes cirúrgicos para um aplicador de clipe cirúrgico, e aplicador de clipe cirúrgico
US10975442B2 (en) 2014-12-19 2021-04-13 Massachusetts Institute Of Technology Molecular biomarkers for cancer immunotherapy
US10993997B2 (en) 2014-12-19 2021-05-04 The Broad Institute, Inc. Methods for profiling the t cell repertoire
IL302102A (en) 2015-05-20 2023-06-01 Dana Farber Cancer Inst Inc shared neoantigens
US10870891B2 (en) 2017-01-05 2020-12-22 Biodesix, Inc. Diagnostic test system for specific, sensitive and reproducible detection of circulating nucleic acids in whole blood
US11549149B2 (en) 2017-01-24 2023-01-10 The Broad Institute, Inc. Compositions and methods for detecting a mutant variant of a polynucleotide
CA3058807A1 (en) 2017-04-03 2018-10-11 Neon Therapeutics, Inc. Protein antigens and uses thereof
CN107287309A (zh) * 2017-07-06 2017-10-24 罗保君 一种血液循环肿瘤dna点突变检测方法
JP6603827B2 (ja) * 2017-07-26 2019-11-06 積水メディカル株式会社 変異遺伝子検出方法
CN109295175A (zh) * 2017-09-08 2019-02-01 广州健天基因技术有限公司 用于检测人胃肠道间质瘤c-kit基因v559a突变的引物、检测方法及试剂盒
CN109306374A (zh) * 2017-09-08 2019-02-05 广州健天基因技术有限公司 用于检测人类pik3ca基因e545k突变的引物、检测方法及试剂盒
JP7306618B2 (ja) * 2017-09-26 2023-07-11 東ソー株式会社 がんの転移形成能に関与する因子および前記因子を用いたがん患者の予後予測方法
TW201930340A (zh) 2017-12-18 2019-08-01 美商尼恩醫療公司 新抗原及其用途
CN107937490A (zh) * 2018-01-15 2018-04-20 福建农林大学 一种非对称pcr的试纸条检测植物病原菌的方法及试剂盒
CN111684078B (zh) * 2018-02-12 2024-04-19 豪夫迈·罗氏有限公司 通过评价肿瘤遗传异质性来预测对治疗的应答的方法
TW202015719A (zh) 2018-06-19 2020-05-01 美商尼恩醫療公司 新抗原及其用途
CN109666747A (zh) * 2019-02-20 2019-04-23 天津脉络医学检验有限公司 一种引物探针组合物及其应用
US11812966B2 (en) 2020-04-24 2023-11-14 NeuraMedica Inc. Clips, appliers, and cartridges
CN113930500B (zh) * 2020-07-13 2024-11-19 铭炽生物科技(上海)有限公司 人pik3ca基因突变的数字pcr检测方法及应用
CN112951325B (zh) * 2021-02-18 2023-04-21 北京吉因加医学检验实验室有限公司 一种用于癌症检测的探针组合的设计方法及其应用
CN113063835B (zh) * 2021-03-19 2022-08-02 中山大学 一种基于元素标记-电感耦合等离子体质谱检测策略的cfDNA分析方法及其应用
WO2023025218A1 (zh) * 2021-08-25 2023-03-02 南京金斯瑞生物科技有限公司 一种用于检测单碱基突变的引物组和基因芯片方法

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PT3556866T (pt) 2004-03-02 2021-04-30 Univ Johns Hopkins Mutações do gene de pik3ca em cancros humanos
US9556491B2 (en) * 2007-02-26 2017-01-31 John Wayne Cancer Institute Utility of B-RAF DNA mutation in diagnosis and treatment of cancer
US8071338B2 (en) * 2007-08-08 2011-12-06 Roche Molecular Systems, Inc. Suppression of amplification using an oligonucleotide and a polymerase significantly lacking 5′-3′ nuclease activity
GB2453173A (en) 2007-09-28 2009-04-01 Dxs Ltd Polynucleotide primers
US20100041048A1 (en) * 2008-07-31 2010-02-18 The Johns Hopkins University Circulating Mutant DNA to Assess Tumor Dynamics
CN101445832B (zh) 2008-12-23 2011-09-14 广州益善生物技术有限公司 Pik3ca基因突变的检测探针、液相芯片及其检测方法
CN102234685B (zh) * 2010-04-23 2014-08-27 益善生物技术股份有限公司 一种pik3ca基因突变检测液相芯片
CN103459612A (zh) 2010-12-03 2013-12-18 布兰代斯大学 用于检测野生型群体中的核酸突变体的方法和试剂盒
CN102533958A (zh) * 2010-12-28 2012-07-04 苏州科贝生物技术有限公司 一种检测人pik3ca基因突变的方法和试剂盒
EP2663652B1 (en) 2011-01-11 2018-11-14 Roche Diagniostics GmbH High resolution melting analysis as a prescreening tool
WO2012131092A2 (en) * 2011-03-31 2012-10-04 Signature Diagnostics Ag Method and kits for the prediction of response/nonresponse to the treatment with an anti-egfr antibody in patients with colorectal cancer of all uicc stages
EP2705162B1 (en) * 2011-05-04 2018-07-11 Biocept, Inc. Methods for detecting nucleic acid sequence variants
KR20140057343A (ko) * 2011-08-18 2014-05-12 네스텍 소시에테아노님 대립형질 변이 검출을 위한 조성물 및 방법
JP2013081450A (ja) 2011-09-27 2013-05-09 Arkray Inc 多型検出用プローブ、多型検出方法、薬効判定方法及び多型検出用試薬キット
US11261494B2 (en) * 2012-06-21 2022-03-01 The Chinese University Of Hong Kong Method of measuring a fractional concentration of tumor DNA
EP2711432A1 (en) 2012-09-21 2014-03-26 Genomica S.A.U. Method for detection of BRAF and PI3K mutations
CN105378160B (zh) * 2013-08-12 2018-04-17 伯乐生命医学产品有限公司 具有碱基配对的寡聚体的扩增报告子

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PL3198026T3 (pl) 2020-05-18
WO2016020710A1 (en) 2016-02-11
PT3198026T (pt) 2020-01-16
CA2957396C (en) 2021-03-09
HRP20192353T1 (hr) 2020-03-20
IL250392B (en) 2020-03-31
US11279979B2 (en) 2022-03-22
SI3198026T1 (sl) 2020-02-28
DK3198026T3 (da) 2020-01-20
LT3198026T (lt) 2020-01-27
JP2017523810A (ja) 2017-08-24
ES2764423T3 (es) 2020-06-03
CY1122647T1 (el) 2021-03-12
EP3198026B1 (en) 2019-10-09
EP3198026A1 (en) 2017-08-02
CN106715723A (zh) 2017-05-24
SMT202000005T1 (it) 2020-03-13
HUE047958T2 (hu) 2020-05-28
RS59710B1 (sr) 2020-01-31
JP6770957B2 (ja) 2020-10-21
US20170233821A1 (en) 2017-08-17
CA2957396A1 (en) 2016-02-11

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