JP6770957B2 - 試料中のpik3ca突然変異状態を決定する方法 - Google Patents

試料中のpik3ca突然変異状態を決定する方法 Download PDF

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JP6770957B2
JP6770957B2 JP2017526770A JP2017526770A JP6770957B2 JP 6770957 B2 JP6770957 B2 JP 6770957B2 JP 2017526770 A JP2017526770 A JP 2017526770A JP 2017526770 A JP2017526770 A JP 2017526770A JP 6770957 B2 JP6770957 B2 JP 6770957B2
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リアニドウ エヴリクレイア
リアニドウ エヴリクレイア
マルコウ アティナ
マルコウ アティナ
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JP2017526770A 2014-08-07 2015-07-28 試料中のpik3ca突然変異状態を決定する方法 Active JP6770957B2 (ja)

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US201462034231P 2014-08-07 2014-08-07
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PCT/GR2015/000036 WO2016020710A1 (en) 2014-08-07 2015-07-28 Method of determining pik3ca mutational status in a sample

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Families Citing this family (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102315754B1 (ko) 2010-05-14 2021-10-22 더 제너럴 하스피톨 코포레이션 종양 특이적 신생항원을 확인하는 조성물 및 방법
WO2015039024A1 (en) 2013-09-16 2015-03-19 Oregon Health & Science University Bioabsorbable clips and applicator for tissue closure
WO2015077717A1 (en) 2013-11-25 2015-05-28 The Broad Institute Inc. Compositions and methods for diagnosing, evaluating and treating cancer by means of the dna methylation status
US11725237B2 (en) 2013-12-05 2023-08-15 The Broad Institute Inc. Polymorphic gene typing and somatic change detection using sequencing data
EP3082853A2 (en) 2013-12-20 2016-10-26 The Broad Institute, Inc. Combination therapy with neoantigen vaccine
CA2965986C (en) 2014-11-03 2019-05-07 Oregon Health & Science University Clips and applicator for tissue closure
WO2016100977A1 (en) 2014-12-19 2016-06-23 The Broad Institute Inc. Methods for profiling the t-cel- receptor repertoire
WO2016100975A1 (en) 2014-12-19 2016-06-23 Massachsetts Institute Ot Technology Molecular biomarkers for cancer immunotherapy
CR20200476A (es) 2015-05-20 2020-12-02 Dana Farber Cancer Inst Inc ANTÍGENOS COMPARTIDOS (Divisional 2017-0584)
EP3358020B1 (en) * 2017-01-05 2021-05-19 Biodesix, Inc. Diagnostic test system for specific, sensitive and reproducible detection of circulating nucleic acids in whole blood
WO2018140391A1 (en) * 2017-01-24 2018-08-02 The Broad Institute, Inc. Compositions and methods for detecting a mutant variant of a polynucleotide
JP7457642B2 (ja) 2017-04-03 2024-03-28 ビオンテック ユーエス インコーポレイテッド タンパク質抗原およびその使用
CN107287309A (zh) * 2017-07-06 2017-10-24 罗保君 一种血液循环肿瘤dna点突变检测方法
KR102662580B1 (ko) * 2017-07-26 2024-05-03 세키스이 메디칼 가부시키가이샤 변이 유전자 검출 방법
CN109295175A (zh) * 2017-09-08 2019-02-01 广州健天基因技术有限公司 用于检测人胃肠道间质瘤c-kit基因v559a突变的引物、检测方法及试剂盒
CN109306374A (zh) * 2017-09-08 2019-02-05 广州健天基因技术有限公司 用于检测人类pik3ca基因e545k突变的引物、检测方法及试剂盒
JP7306618B2 (ja) * 2017-09-26 2023-07-11 東ソー株式会社 がんの転移形成能に関与する因子および前記因子を用いたがん患者の予後予測方法
TW201930340A (zh) 2017-12-18 2019-08-01 美商尼恩醫療公司 新抗原及其用途
CN107937490A (zh) * 2018-01-15 2018-04-20 福建农林大学 一种非对称pcr的试纸条检测植物病原菌的方法及试剂盒
EP3752643B1 (en) * 2018-02-12 2024-01-17 F. Hoffmann-La Roche AG Method of predicting response to therapy by assessing tumor genetic heterogeneity
CA3103983A1 (en) 2018-06-19 2019-12-26 Biontech Us Inc. Neoantigens and uses thereof
CN109666747A (zh) * 2019-02-20 2019-04-23 天津脉络医学检验有限公司 一种引物探针组合物及其应用
US11812966B2 (en) 2020-04-24 2023-11-14 NeuraMedica Inc. Clips, appliers, and cartridges
CN113930500B (zh) * 2020-07-13 2024-11-19 铭炽生物科技(上海)有限公司 人pik3ca基因突变的数字pcr检测方法及应用
CN112951325B (zh) * 2021-02-18 2023-04-21 北京吉因加医学检验实验室有限公司 一种用于癌症检测的探针组合的设计方法及其应用
CN113063835B (zh) * 2021-03-19 2022-08-02 中山大学 一种基于元素标记-电感耦合等离子体质谱检测策略的cfDNA分析方法及其应用
CN117858956A (zh) * 2021-08-25 2024-04-09 南京金斯瑞生物科技有限公司 一种用于检测单碱基突变的引物组和基因芯片方法

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2537633T3 (es) 2004-03-02 2015-06-10 The Johns Hopkins University Mutaciones del gen de PIK3CA en cánceres humanos
US9556491B2 (en) * 2007-02-26 2017-01-31 John Wayne Cancer Institute Utility of B-RAF DNA mutation in diagnosis and treatment of cancer
US8071338B2 (en) 2007-08-08 2011-12-06 Roche Molecular Systems, Inc. Suppression of amplification using an oligonucleotide and a polymerase significantly lacking 5′-3′ nuclease activity
GB2453173A (en) 2007-09-28 2009-04-01 Dxs Ltd Polynucleotide primers
US20100041048A1 (en) * 2008-07-31 2010-02-18 The Johns Hopkins University Circulating Mutant DNA to Assess Tumor Dynamics
CN101445832B (zh) * 2008-12-23 2011-09-14 广州益善生物技术有限公司 Pik3ca基因突变的检测探针、液相芯片及其检测方法
CN102234685B (zh) 2010-04-23 2014-08-27 益善生物技术股份有限公司 一种pik3ca基因突变检测液相芯片
CN103459612A (zh) 2010-12-03 2013-12-18 布兰代斯大学 用于检测野生型群体中的核酸突变体的方法和试剂盒
CN102533958A (zh) 2010-12-28 2012-07-04 苏州科贝生物技术有限公司 一种检测人pik3ca基因突变的方法和试剂盒
WO2012095378A1 (en) 2011-01-11 2012-07-19 Roche Diagnostics Gmbh High resolution melting analysis as a prescreening tool
WO2012131092A2 (en) * 2011-03-31 2012-10-04 Signature Diagnostics Ag Method and kits for the prediction of response/nonresponse to the treatment with an anti-egfr antibody in patients with colorectal cancer of all uicc stages
WO2012151560A2 (en) * 2011-05-04 2012-11-08 Biocept, Inc. Methods for detecting nucleic acid sequence variants
SG2014010276A (en) 2011-08-18 2014-03-28 Nestec Sa Compositions and methods for detecting allelic variants
JP2013081450A (ja) 2011-09-27 2013-05-09 Arkray Inc 多型検出用プローブ、多型検出方法、薬効判定方法及び多型検出用試薬キット
US11261494B2 (en) * 2012-06-21 2022-03-01 The Chinese University Of Hong Kong Method of measuring a fractional concentration of tumor DNA
EP2711432A1 (en) 2012-09-21 2014-03-26 Genomica S.A.U. Method for detection of BRAF and PI3K mutations
CN105378160B (zh) * 2013-08-12 2018-04-17 伯乐生命医学产品有限公司 具有碱基配对的寡聚体的扩增报告子

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DK3198026T3 (da) 2020-01-20
IL250392A0 (en) 2017-03-30
ES2764423T3 (es) 2020-06-03
CN106715723B (zh) 2021-03-30
RS59710B1 (sr) 2020-01-31
EP3198026B1 (en) 2019-10-09
JP2017523810A (ja) 2017-08-24
HRP20192353T1 (hr) 2020-03-20
US20170233821A1 (en) 2017-08-17
CY1122647T1 (el) 2021-03-12
EP3198026A1 (en) 2017-08-02
WO2016020710A1 (en) 2016-02-11
CA2957396C (en) 2021-03-09
SI3198026T1 (sl) 2020-02-28
PL3198026T3 (pl) 2020-05-18
US11279979B2 (en) 2022-03-22
SMT202000005T1 (it) 2020-03-13
CA2957396A1 (en) 2016-02-11

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