CN106699410A - Bio-organic fertilizer for improving soil microbial functional diversity and production method thereof - Google Patents
Bio-organic fertilizer for improving soil microbial functional diversity and production method thereof Download PDFInfo
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- CN106699410A CN106699410A CN201710066163.3A CN201710066163A CN106699410A CN 106699410 A CN106699410 A CN 106699410A CN 201710066163 A CN201710066163 A CN 201710066163A CN 106699410 A CN106699410 A CN 106699410A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F1/00—Fertilisers made from animal corpses, or parts thereof
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Abstract
The invention discloses a bio-organic fertilizer for improving soil microbial functional diversity and a production method thereof, wherein the production method comprises the steps: step one, taking fallow soil, and adjusting the moisture to 40%-60%; step two, spreading 12% of a vinasse organic fertilizer, 4% of an earthworm organic fertilizer, 12% of a drug residue organic fertilizer, 8% of an oil cake organic fertilizer and 8% of a cow manure organic fertilizer based on the fallow soil mass, mixing evenly, and building into a strip pile; step three, fermenting for 1-2 days, and when the strip pile temperature rises to 60-65 DEG C, turning over the pile for a first time; step four, 2-3 days later, when the strip pile temperature rises to 60-65 DEG C again, turning over the pile again; and step five, fermenting for 8-10 days again, and when the temperature rises no longer, completing the preparation of the bio-organic fertilizer. Microorganisms in the fallow soil can be well bred and amplified; when the bio-organic fertilizer is applied in farming soil, the quantity and function of soil microorganisms can be significantly increased, the growth environment of crops is optimized, and then the yield of the crops is increased.
Description
Technical field
The present invention relates to a kind of biological organic fertilizer for improving edaphon functional diversity and preparation method thereof, category
In technical field of biological organic fertilizer.
Background technology
Biological organic fertilizer refer to microorganisms with specific functions with mainly with plant and animal residues (such as feces of livestock and poultry, agricultural crop straw
Deng) it is to originate and have microbial manure and organic fertilizer efficiency concurrently through the class that harmless treatment, the organic materials for becoming thoroughly decomposed are composited
The fertilizer answered.In addition to containing organic matter higher, also containing the microorganism with specific function, this is bio-organic fertilizer product
The substantive characteristics of such product.And contained microorganism should show certain fertilizer effect, such as have and promote soil fertility, system
Make and assist that crops absorb nutrition, the compound of indissoluble is made for thing and the effect such as absorbs in activating soil, or can produce many
Active material and anti-, suppression disease substance are planted, has good stimulation and regulating and controlling effect to the growth of crops, can reduce or reduce crop
The generation of pest and disease damage, and improve quality of agricultural product.
Compared with common organic fertilizer, the production of biological organic fertilizer promotes organic materials rotten except to be added in digest process
Bacteria agent that is ripe, decomposing, to realize that orientation is become thoroughly decomposed, outside the purpose such as deodorant, also needs to add with specific function in the product
Microorganism, to lift the action effect of product.
Research discovery, the soil of long-term use chemical fertilizer is considerably better than in the crop of the grown on soil lain fallow for a long time, wherein
Lie fallow edaphon structure, functional diversity have significant difference, soil resource of lying fallow for a long time is limited can not to be largely used to
Production, therefore, how the microorganism in soil of lying fallow is expanded and then be made biological organic fertilizer by research uses with important reality
Meaning.
The content of the invention
The technical problem to be solved in the present invention is：Offer is a kind of to be had for improving the biology of edaphon functional diversity
Microorganism in soil of lying fallow can be cultivated and be expanded by machine fertilizer and preparation method thereof, the biological organic fertilizer well, application
The quantity and feature of edaphon can be significantly improved in anthropogenic soil, and then the growing environment of crop can be optimized, with
Overcome the deficiencies in the prior art.
Technical scheme：A kind of biological organic fertilizer condiment, calculates by weight, the biological organic fertilizer condiment master
To be made up of lees organic fertilizer, earthworm fertilizer, dregs of a decoction organic fertilizer, oil cake organic fertilizer and cow dung organic fertilizer.
Preferably, calculate by weight, the biological organic fertilizer condiment is main by 12 parts of lees organic fertilizer, earthworm fertilizer
8 parts of 4 parts, 12 parts of dregs of a decoction organic fertilizer, 8 parts of oil cake organic fertilizer and cow dung organic fertilizer are constituted.
A kind of biological organic fertilizer, calculates by weight, and the soil that will lie fallow is with biological organic fertilizer condiment by 100：44 are formulated.
The preparation method of biological organic fertilizer is comprised the following steps：
Step one：Take soil regulation moisture to 40%~60% of lying fallow；
Step 2：Biological organic fertilizer condiment is uniformly sprinkling upon and is lain fallow on soil, bar pile is built up after being well mixed；
Step 3：Fermentation 1~2 day, first time turning is carried out when bar pile temperature is raised to 60~65 DEG C；
Step 4：After spending 2~3 days, turning is carried out again when bar pile temperature is raised to 60~65 DEG C again；
Step 5：Ferment again 8~10 days, the preparation of biological organic fertilizer is completed after temperature no longer rises.
In abovementioned steps, temperature raises the microorganism that explanation lies fallow in soil and is carrying out amount reproduction, if constant temperature
Rising will reduce the quantity of microorganism in biological organic fertilizer, therefore method using turning is lowered the temperature, until expand it is numerous after
Just there is no number change after micro organism quantity stabilization, temperature will not also be raised.
The beneficial effects of the invention are as follows：Biological organic fertilizer of the present invention is by will lie fallow soil and biological organic fertilizer condiment by certain
Proportions are formed, and the microorganism in soil of lying fallow can be cultivated and be expanded well, are applied to energy in anthropogenic soil
The quantity and feature of edaphon are enough significantly improved, optimizes the growing environment of crop, and then the yield of crop can be improved.
The biological organic fertilizer being made up of the present invention reaches national biological organic fertilizer standard, and micro organism quantity and feature are significantly better than state
Family's standard.Tested according to applicant, this biological organic fertilizer parameter is organic matter 56%, N, P2O5、K2O is respectively 3%, 2.18%,
1.29%, total nutrient 6.47%, pH=6.2, moisture is 29%, and induced worm egg death rate and excrement colibacillus group number index meet NY
The requirement of 884-2012.Living bacteria count (CFU) >=0.3 hundred million/gram
In the present invention, lees organic fertilizer and oil cake organic fertilizer are mainly as microorganism nitrogen source in the numerous soil of lying fallow of expansion
Source, dregs of a decoction organic fertilizer and cow dung organic fertilizer are mainly as the source for expanding microbe carbon source in numerous soil of lying fallow, and earthworm fertilizer is main
Expand the active material that numerous Tu Zhong Institute of Micro-biology of lying fallow needs as providing.
Specific embodiment
To make the object, technical solutions and advantages of the present invention clearer, present invention work is further retouched in detail below
State.
First, embodiment
Embodiment 1：
A kind of biological organic fertilizer condiment, calculates by weight, and the biological organic fertilizer condiment is main by lees organic fertilizer, earthworm
Earthworm organic fertilizer, dregs of a decoction organic fertilizer, oil cake organic fertilizer and cow dung organic fertilizer mixing composition.
Embodiment 2：
A kind of biological organic fertilizer condiment, calculates by weight, and the biological organic fertilizer condiment is main by lees organic fertilizer 12
Part, 4 parts of earthworm fertilizer, 12 parts of dregs of a decoction organic fertilizer, 8 parts of oil cake organic fertilizer and 8 parts of compositions of cow dung organic fertilizer.
Embodiment 3：
A kind of biological organic fertilizer condiment, calculates by weight, and the biological organic fertilizer condiment is main by lees organic fertilizer 10
Part, 5 parts of earthworm fertilizer, 4 parts of dregs of a decoction organic fertilizer, 3 parts of oil cake organic fertilizer and 6 parts of compositions of cow dung organic fertilizer.
Embodiment 4：
A kind of biological organic fertilizer condiment, calculates by weight, and the biological organic fertilizer condiment is main by lees organic fertilizer 8
Part, 10 parts of earthworm fertilizer, 7 parts of dregs of a decoction organic fertilizer, 5 parts of oil cake organic fertilizer and 4 parts of compositions of cow dung organic fertilizer.
Embodiment 5：
A kind of preparation method of biological organic fertilizer, comprises the following steps：
Step one：Take after soil of lying fallow goes the removal of impurity and adjust moisture to 40%~60%；
Step 2：Calculate by weight, the soil that will lie fallow is with biological organic fertilizer condiment by 100：44 prepare, will 44 parts of life
Organic fertilizer condiment is uniformly sprinkling upon 100 parts and lies fallow on soil, it is well mixed after build up bar pile；
Step 3：Fermentation 1~2 day, first time turning is carried out when bar pile temperature is raised to 60~65 DEG C；
Step 4：After spending 2~3 days, turning is carried out again when bar pile temperature is raised to 60~65 DEG C again；
Step 5：Ferment again 8~10 days, the preparation of biological organic fertilizer is completed after temperature no longer rises.
Result and analysis：After aforementioned biological organic fertilizer is applied into anthropogenic soil, optimal group (embodiment 2 is matched) and blank
Group, non-optimal group (proportioning of embodiment 3,4) are compared, and quantity and the functional data contrast of edaphon are as follows：
The field experiment of table 1
| Shannon diversity indices | Simpson indexes | McIntosh indexes | |
| Blank soil | 1.97±1.14b | 20.15±4.89b | 650.47±52.23c |
| Non-optimal group | 2.29±1.04ab | 22.71±5.12b | 807.21±45.35b |
| Optimal group | 3.28±0.73a | 46.12±2.63a | 974.56±63.97a |
In addition, in the present invention, foregoing lees organic fertilizer, earthworm fertilizer, cow dung organic fertilizer, oil cake organic fertilizer, the dregs of a decoction
Organic fertilizer nutrient is as shown in table 2.
The various organic fertilizer nutrients of table 2
2nd, design experiment and analysis method
1st, materials and methods
1.1 materials to be tested
For examination soil：The 10 years soil of lying fallow in Guiyang City, Guizhou Province Kaiyang County Hua Li townshiies.
For examination fertilizer：Lees organic fertilizer is provided by Jinsha County tobacco monopoly bureau；Earthworm fertilizer and cow dung organic fertilizer are by expensive
Zhou Jilong Eco Science Technologies Co., Ltd provides；Oil cake organic fertilizer is provided by the south of Guizhou Province Jin Fu Co., Ltds, and dregs of a decoction organic fertilizer is by Guizhou
Tobacco scientific research is saved to provide.
Other materials to be tested：River sand, ECO plates.
For test instrument：Tissue culture bottle, watering can, autoclave (TOMY Autoclave SX-500), water purification machine (excellent general XB100),
Growth cabinet (Sanyo MLR-351H, BOXUN BSG-400), shaking table (HYL-C combined types shaking table), superclean bench (Suzhou
Purification SW-CJ-2F), triangular flask, Omnilog PM high-flux microorganism cell phenotype chip measurement systems.
1.2 test methods
1.2.1 the determination of test index, influence factor
Using Shannon diversity indices, Simpson indexes, McIntosh indexes as test index；Five kinds of selection
Fertilizer is used as influence factor：Lees organic fertilizer, earthworm fertilizer, dregs of a decoction organic fertilizer, oil cake organic fertilizer and cow dung organic fertilizer.
1.2.2 the determination of test process
Experience takes 4 levels and is tested in the characteristics of according to factor and single factor experiment：0%th, 4%, 8%, 12% (table
Show this kind of fertilizer accounting), lie fallow soil add 30%, remainder by sterilizing after yellow sand supply.According to orthogonal test table to examination
The orthogonal arrage tested after optimization is as follows：
Orthogonal table
1.2.3 the setting of experimental condition
The relative water-holding capacity for adjusting test process is 60%；Regulation growth cabinet temperature is 30 DEG C, air humidity is
70%；Cultivation cycle is 30 days.
1.2.4 assay method
At the 30th day, each treatment took 2g soil samples, is put into the sterilized triangular flask equipped with 18mL water, is placed in and shakes
Bed concussion 30min (170 revs/min), be placed in after the completion of concussion super-clean bench standing, take supernatant dilute 50 times, mix, point sample in
On ECO plates, Omnilog PM high-flux microorganism cell phenotype chip measurement systems culture four days is put into, correlation is drawn after four days
Data.
Index analysis method analyze data according to ECO plates, analysis method is as follows：
Diversity index is parsed
1.2.5 data analysing method
The variation that factor causes and variation two parts that error causes are resolved into total variation of data, F statistics are constructed,
Make F inspections, you can whether significantly factor of judgment effect (substitutes into SPSS, calculate into following index)：
Sum of square of deviations is decomposed：Square sum of total departure=each row multiple factor bias quadratic sum+error deviation quadratic sum
The empty row (error) of SST=SS factors+SS
The free degree is decomposed：
The empty row (error) of dfT=df factors+df
Variance：
Construction F statistics：
Row analysis of variance table, makees F inspections.
2nd, result and analysis
2.1 orthogonal experiment datas
Orthogonal experiment initial data (average value)
2.3 data analyses and result
2.3.1Shannon diversity indices
Index：Dependent variable:Shannon diversity indices
| Source | Type III quadratic sum | df | It is square | F | Sig. | Conspicuousness |
| Calibration model | 2.285 | 15 | .152 | 3.684 | .001 | - |
| Intercept | 476.709 | 1 | 476.709 | 11527.935 | .000 | - |
| Lees organic fertilizer | .671 | 3 | .224 | 5.409 | .004 | ** |
| Earthworm fertilizer | .294 | 3 | .098 | 2.371 | .089 | It is not notable |
| Dregs of a decoction organic fertilizer | .175 | 3 | .058 | 1.407 | .259 | It is not notable |
| Oil cake organic fertilizer | .181 | 3 | .060 | 1.459 | .244 | It is not notable |
| Cow dung organic fertilizer | .550 | 3 | .183 | 4.436 | .010 | ** |
| Error | 1.323 | 32 | .041 | - | - | - |
| Amount to | 430.317 | 48 | - | - | - | - |
| The total of correction | 3.608 | 47 | - | - | - | - |
A.R side=.633 (adjustment R side=.461)
* represent notable, * * represent extremely notable
After being analyzed to the result with Shannon diversity indices as index, it can be found that by wherein wine
The sig values of poor organic fertilizer are less than 0.01, and conspicuousness is extremely notable；Cow dung organic fertilizer sig values are less than 0.05, are the level of signifiance；Earthworm
The sig values of earthworm organic fertilizer, dregs of a decoction organic fertilizer and oil cake organic fertilizer are more than 0.05, and conspicuousness is not notable.
Again by contrasting the type III quadratic sum between five kinds of fertilizer, it can be found that the importance ranking between five kinds of fertilizer
For：Lees organic fertilizer>Cow dung organic fertilizer>Earthworm fertilizer>The withered organic fertilizer of medicine oil>Dregs of a decoction organic fertilizer.
After importance ranking determines, it is necessary to determine Ju Ti Pei Fang according to the marginal average of every kind of soil fertility quality：
After five kinds of importance of organic fertilizer have above been analyzed, it is necessary to draw Ju Ti Pei Fang according to data analysis, such as
Under：By the way that five kinds of marginal averages of fertilizer can be drawn after the analysis to orthogonal experiment data, lees organic fertilizer can be drawn accordingly
Optimal proportion is that the optimal proportion of 12%, earthworm fertilizer is that the 4%, optimal proportion of dregs of a decoction organic fertilizer is that 12%, oil cake is organic
The optimal proportion of fertilizer is that the 8%, optimal proportion of cow dung organic fertilizer is 8%, i.e., optimization formula is that lees organic fertilizer 12%, earthworm has
Machine fertilizer 4%, dregs of a decoction organic fertilizer 12%, oil cake organic fertilizer 8%, cow dung organic fertilizer 8%.
2.3.2Simpson index
Index：Simpson indexes
A.R side=.731 (adjustment R side=.605)
* represent notable, * * represent extremely notable
After being analyzed to the result with Simpson indexes as index, it can be found that organic by wherein vinasse
The sig values of fertilizer and cow dung organic fertilizer are less than 0.01, and conspicuousness is extremely notable；The sig values of earthworm fertilizer are less than 0.05, are notable
Level；The sig values of dregs of a decoction organic fertilizer and oil cake organic fertilizer are more than 0.05, and conspicuousness is not notable.
Again by contrasting the type III quadratic sum between five kinds of fertilizer, it can be found that the importance ranking between five kinds of fertilizer
For：Lees organic fertilizer>Cow dung organic fertilizer>Earthworm fertilizer>Dregs of a decoction organic fertilizer>Oil cake organic fertilizer.
After importance ranking determines, it is necessary to determine Ju Ti Pei Fang according to the marginal average of every kind of soil fertility quality：
By the way that five kinds of marginal averages of fertilizer can be drawn after the analysis to orthogonal experiment data, can show that vinasse are organic accordingly
The optimal proportion of fertilizer is that the optimal proportion of 12%, earthworm fertilizer is that the 4%, optimal proportion of dregs of a decoction organic fertilizer is 12%, oil cake
The optimal proportion of organic fertilizer is that the 8%, optimal proportion of cow dung organic fertilizer is 8%, i.e., optimization formula is lees organic fertilizer 12%, earthworm
Earthworm organic fertilizer 4%, dregs of a decoction organic fertilizer 12%, oil cake organic fertilizer 8%, cow dung organic fertilizer 8%.
2.3.13McIntosh index
Index：McIntosh indexes
A.R side=.562 (adjustment R side=.356)
* represent notable, * * represent extremely notable
After being analyzed to the result with McIntosh indexes as index, it can be found that organic by wherein vinasse
The sig values of fertilizer are less than 0.01, and conspicuousness is extremely notable；The sig values of cow dung organic fertilizer are less than 0.05, and conspicuousness is notable；Earthworm
The sig values of organic fertilizer, dregs of a decoction organic fertilizer and oil cake organic fertilizer are more than 0.05, and notable nature and flavor are not notable.
Again by contrasting the type III quadratic sum between five kinds of fertilizer, it can be found that the importance ranking between five kinds of fertilizer
For：Lees organic fertilizer>Cow dung organic fertilizer>Earthworm fertilizer>Oil cake organic fertilizer>Dregs of a decoction organic fertilizer.
After importance ranking determines, it is necessary to determine Ju Ti Pei Fang according to the marginal average of every kind of soil fertility quality：
By the way that five kinds of marginal averages of fertilizer can be drawn after the analysis to orthogonal experiment data, can show that vinasse are organic accordingly
The optimal proportion of fertilizer is that the optimal proportion of 12%, earthworm fertilizer is that the 4%, optimal proportion of dregs of a decoction organic fertilizer is 12%, oil cake
The optimal proportion of organic fertilizer is that the 8%, optimal proportion of cow dung organic fertilizer is 8%, i.e., optimization formula is lees organic fertilizer 12%, earthworm
Earthworm organic fertilizer 4%, dregs of a decoction organic fertilizer 12%, oil cake organic fertilizer 8%, cow dung organic fertilizer 8%.
Above content is to combine specific preferred embodiment further description made for the present invention, it is impossible to assert
Specific implementation of the invention is confined to these explanations.For general technical staff of the technical field of the invention,
On the premise of not departing from present inventive concept, some simple deduction or replace can also be made, should be all considered as belonging to of the invention
Protection domain.
Claims (4)
1. a kind of biological organic fertilizer condiment, it is characterised in that：Calculate by weight, the biological organic fertilizer condiment is main by vinasse
Organic fertilizer, earthworm fertilizer, dregs of a decoction organic fertilizer, oil cake organic fertilizer and cow dung organic fertilizer composition.
2. biological organic fertilizer condiment according to claim 1, it is characterised in that：Calculate by weight, the biological organic
Fertile condiment is mainly had by 12 parts of lees organic fertilizer, 4 parts of earthworm fertilizer, 12 parts of dregs of a decoction organic fertilizer, 8 parts of oil cake organic fertilizer and cow dung
8 parts of compositions of machine fertilizer.
3. a kind of biological organic fertilizer, it is characterised in that：Calculate by weight, will lie fallow soil and the biology described in claim 1 or 2
Organic fertilizer condiment presses 100：44 are formulated.
4. the preparation method of biological organic fertilizer as claimed in claim 3, it is characterised in that：Comprise the following steps：
Step one：Take soil regulation moisture to 40%~60% of lying fallow；
Step 2：Biological organic fertilizer condiment is uniformly sprinkling upon and is lain fallow on soil, bar pile is built up after being well mixed；
Step 3：Fermentation 1~2 day, first time turning is carried out when bar pile temperature is raised to 60~65 DEG C；
Step 4：After spending 2~3 days, turning is carried out again when bar pile temperature is raised to 60~65 DEG C again；
Step 5：Ferment again 8~10 days, the preparation of biological organic fertilizer is completed after temperature no longer rises.
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Cited By (1)
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| CN107445762A (en) * | 2017-08-07 | 2017-12-08 | 南京农业大学 | A kind of soil conditioner and preparation method thereof |
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| CN106699410B (en) | 2020-08-04 |
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Inventor after: Liu Yanxia Inventor after: Yang Xingming Inventor after: Li Xiang Inventor after: Chen Zhiwen Inventor after: Shi Junxiong Inventor after: Sun Guangjun Inventor before: Liu Yanxia Inventor before: Li Xiang Inventor before: Chen Zhiwen Inventor before: Shi Junxiong |
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| CB03 | Change of inventor or designer information |